Spg004104 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg004104
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold4: 1043588 .. 1044655 (+)
RNA-Seq ExpressionSpg004104
SyntenySpg004104
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTCCTCTCCAGAAAGCTCCTCTCCGACGACGACAACCTCAAGAAATTCTGCGATGATTACTGCGACCGTTTCCCTTCCGATTCTTGCCCTTCCGGCTGCCTTGAAAACTGCCCAAAACAATGTCCATATTTGTCGATTCCAATCCCACCGCCCAATTACGACGATCTCAATCCCAATTCCCCATCAAAATCGCCTCACAAACTCTCCACTTTGCTCACCGTCACCATCGCCCTTCTCTCCTTCACCTTTCTCCTCGTCCTCTGCTACGCCGTCTACGTCCGTTTCTACTCCGGCCGCCGCAGGCGCCGCCGTCGCGGCCCTGATGCCCCGCCGGAGGCCACCCACCAGCAGGAGTTTCTCGACGAAGAACACGGCCCGGTTCTGGACCACCCCATCTGGTACATCCGCACCATCGGCCTCCCGCCGGCGGTCATCGACGCCATCGCCGTCTGCAAGTTCAAATCCGGCGAGGGATTGATCGACGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCAAGAGGATGAGACTCTGAGGCTTTTGCCCAAGTGCAGCCACGCTTTTCATCTCCCCTGCATCGACACTTGGCTTCGATCCCACACAAACTGCCCCATGTGCCGTGCTCCGGTGGTGGCTCAATCCGCCGCCGTCGCCCCTCCGCCTCCGGTGGCGGCCTCTGAAGAAACCCAGATCGGGGATTTGCAAAGGGAAGTGGGAGATGAGGCCAATTTGGGGGAAAGTTCTCAGACAAACGAAGAACAAGAACAAGAACGAGATGAAATTCATTTGCCCGATGATGATTCAGAGGAAAGAGTTGGTGATTTTCAGCCCATGAGAAGATCCGTGTCTCTAGATTCTTTATCAGCTCTTCGAATCAGCTCTGTTTTCGCTGGTTTTAATGAAAAAACGGATTGTGAAGCTGAAATCGAGCAGAGAAATGGGAATGGATCGAAGAAATCATGGCAGAGGAGAGGGAATTGCTCTGCTGCTATGAACAGGTCAGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGCTCCACTCAGAGAAGTAATCACAGTTGA

mRNA sequence

ATGGATTTCCTCTCCAGAAAGCTCCTCTCCGACGACGACAACCTCAAGAAATTCTGCGATGATTACTGCGACCGTTTCCCTTCCGATTCTTGCCCTTCCGGCTGCCTTGAAAACTGCCCAAAACAATGTCCATATTTGTCGATTCCAATCCCACCGCCCAATTACGACGATCTCAATCCCAATTCCCCATCAAAATCGCCTCACAAACTCTCCACTTTGCTCACCGTCACCATCGCCCTTCTCTCCTTCACCTTTCTCCTCGTCCTCTGCTACGCCGTCTACGTCCGTTTCTACTCCGGCCGCCGCAGGCGCCGCCGTCGCGGCCCTGATGCCCCGCCGGAGGCCACCCACCAGCAGGAGTTTCTCGACGAAGAACACGGCCCGGTTCTGGACCACCCCATCTGGTACATCCGCACCATCGGCCTCCCGCCGGCGGTCATCGACGCCATCGCCGTCTGCAAGTTCAAATCCGGCGAGGGATTGATCGACGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCAAGAGGATGAGACTCTGAGGCTTTTGCCCAAGTGCAGCCACGCTTTTCATCTCCCCTGCATCGACACTTGGCTTCGATCCCACACAAACTGCCCCATGTGCCGTGCTCCGGTGGTGGCTCAATCCGCCGCCGTCGCCCCTCCGCCTCCGGTGGCGGCCTCTGAAGAAACCCAGATCGGGGATTTGCAAAGGGAAGTGGGAGATGAGGCCAATTTGGGGGAAAGTTCTCAGACAAACGAAGAACAAGAACAAGAACGAGATGAAATTCATTTGCCCGATGATGATTCAGAGGAAAGAGTTGGTGATTTTCAGCCCATGAGAAGATCCGTGTCTCTAGATTCTTTATCAGCTCTTCGAATCAGCTCTGTTTTCGCTGGTTTTAATGAAAAAACGGATTGTGAAGCTGAAATCGAGCAGAGAAATGGGAATGGATCGAAGAAATCATGGCAGAGGAGAGGGAATTGCTCTGCTGCTATGAACAGGTCAGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGCTCCACTCAGAGAAGTAATCACAGTTGA

Coding sequence (CDS)

ATGGATTTCCTCTCCAGAAAGCTCCTCTCCGACGACGACAACCTCAAGAAATTCTGCGATGATTACTGCGACCGTTTCCCTTCCGATTCTTGCCCTTCCGGCTGCCTTGAAAACTGCCCAAAACAATGTCCATATTTGTCGATTCCAATCCCACCGCCCAATTACGACGATCTCAATCCCAATTCCCCATCAAAATCGCCTCACAAACTCTCCACTTTGCTCACCGTCACCATCGCCCTTCTCTCCTTCACCTTTCTCCTCGTCCTCTGCTACGCCGTCTACGTCCGTTTCTACTCCGGCCGCCGCAGGCGCCGCCGTCGCGGCCCTGATGCCCCGCCGGAGGCCACCCACCAGCAGGAGTTTCTCGACGAAGAACACGGCCCGGTTCTGGACCACCCCATCTGGTACATCCGCACCATCGGCCTCCCGCCGGCGGTCATCGACGCCATCGCCGTCTGCAAGTTCAAATCCGGCGAGGGATTGATCGACGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCAAGAGGATGAGACTCTGAGGCTTTTGCCCAAGTGCAGCCACGCTTTTCATCTCCCCTGCATCGACACTTGGCTTCGATCCCACACAAACTGCCCCATGTGCCGTGCTCCGGTGGTGGCTCAATCCGCCGCCGTCGCCCCTCCGCCTCCGGTGGCGGCCTCTGAAGAAACCCAGATCGGGGATTTGCAAAGGGAAGTGGGAGATGAGGCCAATTTGGGGGAAAGTTCTCAGACAAACGAAGAACAAGAACAAGAACGAGATGAAATTCATTTGCCCGATGATGATTCAGAGGAAAGAGTTGGTGATTTTCAGCCCATGAGAAGATCCGTGTCTCTAGATTCTTTATCAGCTCTTCGAATCAGCTCTGTTTTCGCTGGTTTTAATGAAAAAACGGATTGTGAAGCTGAAATCGAGCAGAGAAATGGGAATGGATCGAAGAAATCATGGCAGAGGAGAGGGAATTGCTCTGCTGCTATGAACAGGTCAGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGCTCCACTCAGAGAAGTAATCACAGTTGA

Protein sequence

MDFLSRKLLSDDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTNEEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSVFAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCSAAMNRSVSCSGKFLMRSSTQRSNHS
Homology
BLAST of Spg004104 vs. NCBI nr
Match: XP_008455131.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0031432.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK06883.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 477.2 bits (1227), Expect = 1.2e-130
Identity = 266/359 (74.09%), Postives = 286/359 (79.67%), Query Frame = 0

Query: 1   MDFL-SRKLLS---DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYD 60
           MDFL SRKLLS   +DD+LKK C D C  + S SCPS CLE C   C Y+ IPIPPP+ D
Sbjct: 1   MDFLSSRKLLSSDDEDDDLKKLCKD-CFDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDAD 60

Query: 61  DLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT 120
           D+N  S +KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR R     PPE  
Sbjct: 61  DINQTSSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETH 120

Query: 121 HQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQE 180
           HQ EFLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+E
Sbjct: 121 HQHEFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEE 180

Query: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ-SAAVAPPPPVAASEETQIGD 240
           DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ SAA A PPP     ETQIG+
Sbjct: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGE 240

Query: 241 LQREVGDEANLGESSQTNE---EQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
           LQRE GD ANLGESS  NE   EQ+ E +E H  D+D EER  D QP+RRSVSLDSLSAL
Sbjct: 241 LQREEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSAL 300

Query: 301 RISSVFAGFNEKTDCEAEIEQRNGNGSKKSWQRRG-NCSAAMNRSVSCSGKFLMRSSTQ 351
           RISSVFA  NEK D +AE  QR G   KKS Q RG + +++MNRSVSCSGKFLMRSSTQ
Sbjct: 301 RISSVFANVNEKLDSDAETIQRKG---KKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQ 354

BLAST of Spg004104 vs. NCBI nr
Match: XP_038888113.1 (E3 ubiquitin-protein ligase RING1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 473.8 bits (1218), Expect = 1.3e-129
Identity = 259/353 (73.37%), Postives = 284/353 (80.45%), Query Frame = 0

Query: 1   MDFLS-RKLLSDD-DNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF+S RKLLSDD D+++KFC D C  + + SC S C+E CPK C Y+S+ IPPP     
Sbjct: 1   MDFISTRKLLSDDEDDIEKFCKD-CHEYLTGSCSSDCIEKCPKLC-YISMSIPPP----- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT-H 120
               PSKSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR RR PD PP  T H
Sbjct: 61  ----PSKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRR-PDPPPPVTHH 120

Query: 121 QQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQED 180
           Q +FLDEE GPVLDHPIWYIRT+GLPPAVI+AIAVCKFKSGEGLIDGTECSVCLSEF+ED
Sbjct: 121 QHDFLDEEQGPVLDHPIWYIRTVGLPPAVIEAIAVCKFKSGEGLIDGTECSVCLSEFEED 180

Query: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQ 240
           ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ  A A PPP     ETQ+ + Q
Sbjct: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQPTAAASPPPPQPPAETQMREWQ 240

Query: 241 REVGDEANLGESSQTNEEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSV 300
           R  GD ANLGESS  NEEQEQE+ EIH P++D EE     QP+RRS+SLDSLSALRISSV
Sbjct: 241 RGEGDGANLGESSTRNEEQEQEQVEIHFPEEDLEE----IQPVRRSISLDSLSALRISSV 300

Query: 301 FAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCSAAMNRSVSCSGKFLMRSSTQ 351
           FA  NEK D EAE  QRNGN SKKS+Q RGN S+AMNRSVSCSGKFLMR S +
Sbjct: 301 FANVNEKMDSEAESMQRNGNESKKSYQGRGNSSSAMNRSVSCSGKFLMRRSAE 337

BLAST of Spg004104 vs. NCBI nr
Match: XP_004137067.1 (RING-H2 finger protein ATL54 [Cucumis sativus] >KGN43773.1 hypothetical protein Csa_017129 [Cucumis sativus])

HSP 1 Score: 468.0 bits (1203), Expect = 7.2e-128
Identity = 264/361 (73.13%), Postives = 283/361 (78.39%), Query Frame = 0

Query: 1   MDFL-SRKLLS-DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF  SRKLLS DDD+ K+FC D  D F SDSC S CLE C   C  ++IPI PP+ D +
Sbjct: 1   MDFFSSRKLLSEDDDDHKEFCKDCRDYF-SDSCSSDCLEKCRDYCYNINIPIRPPDADHI 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
           NP S  KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFY+GRRRR R     PPE  HQ
Sbjct: 61  NPTSSIKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYAGRRRRIRLPDPPPPETHHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
            +FLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+EDE
Sbjct: 121 HDFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS-AAVAPPPPVAASEETQIGDLQ 240
           TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS AA A PP +    ETQIG+LQ
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAAAASPPQLRPPAETQIGELQ 240

Query: 241 REVGDEANLGESSQTNEE--QEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRIS 300
           RE GD ANLGESS   EE  +EQE  E H  D+D EER  D QPMRRSVSLDSLSALRIS
Sbjct: 241 REEGDGANLGESSARTEEEQEEQEEQETHFADEDLEERSSDIQPMRRSVSLDSLSALRIS 300

Query: 301 SVFAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCS-AAMNRSVSCSGKFLMRSSTQRSNH 356
           S FA  NEK D EAE  QRNG   KKS Q RG+ S ++MNRSVSCS KFLMRSSTQ +  
Sbjct: 301 SDFANVNEKMDSEAETIQRNG---KKSSQGRGHSSTSSMNRSVSCSAKFLMRSSTQINTE 357

BLAST of Spg004104 vs. NCBI nr
Match: XP_038888112.1 (E3 ubiquitin-protein ligase RING1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 449.9 bits (1156), Expect = 2.0e-122
Identity = 246/337 (73.00%), Postives = 270/337 (80.12%), Query Frame = 0

Query: 1   MDFLS-RKLLSDD-DNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF+S RKLLSDD D+++KFC D C  + + SC S C+E CPK C Y+S+ IPPP     
Sbjct: 1   MDFISTRKLLSDDEDDIEKFCKD-CHEYLTGSCSSDCIEKCPKLC-YISMSIPPP----- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT-H 120
               PSKSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR RR PD PP  T H
Sbjct: 61  ----PSKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRR-PDPPPPVTHH 120

Query: 121 QQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQED 180
           Q +FLDEE GPVLDHPIWYIRT+GLPPAVI+AIAVCKFKSGEGLIDGTECSVCLSEF+ED
Sbjct: 121 QHDFLDEEQGPVLDHPIWYIRTVGLPPAVIEAIAVCKFKSGEGLIDGTECSVCLSEFEED 180

Query: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQ 240
           ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ  A A PPP     ETQ+ + Q
Sbjct: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQPTAAASPPPPQPPAETQMREWQ 240

Query: 241 REVGDEANLGESSQTNEEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSV 300
           R  GD ANLGESS  NEEQEQE+ EIH P++D EE     QP+RRS+SLDSLSALRISSV
Sbjct: 241 RGEGDGANLGESSTRNEEQEQEQVEIHFPEEDLEE----IQPVRRSISLDSLSALRISSV 300

Query: 301 FAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCSAAMN 335
           FA  NEK D EAE  QRNGN SKKS+Q RGN S+AMN
Sbjct: 301 FANVNEKMDSEAESMQRNGNESKKSYQGRGNSSSAMN 321

BLAST of Spg004104 vs. NCBI nr
Match: KAG7022535.1 (RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.7 bits (1140), Expect = 1.5e-120
Identity = 250/361 (69.25%), Postives = 271/361 (75.07%), Query Frame = 0

Query: 1   MDFLSRKLLS--DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDFLSRKLLS  DDD+LKK C   C  +  DSCPS CLE CP  C   SI IPPP YD  
Sbjct: 1   MDFLSRKLLSDDDDDDLKKLCLKTCPNYSPDSCPSNCLEKCPNLCSRGSISIPPPYYD-- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
                 KSPHKLS  +T TIA LSFTFLLVLCY VYVRFYSGRRRR +R PD PP  THQ
Sbjct: 61  ------KSPHKLSNWVTATIAFLSFTFLLVLCYTVYVRFYSGRRRRHQR-PDPPPPTTHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
           +EFLDEEHGPVLDHPIWYIRTIGLPPAVI+AIA+CKFK+GEGLIDGTECSVCLSEF+EDE
Sbjct: 121 EEFLDEEHGPVLDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVA-----PPPPVAASEETQI 240
           TLRLLPKCSHAFHLPCIDTWL+SHTNCPMCRAPVVAQS AV      PPPP+  SEETQ+
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQM 240

Query: 241 GDLQREVGDEANLGESSQTNEEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALR 300
            DLQREVG+ +N GESS  NEEQ QE+DEI              QP+RRSVSLDSLSALR
Sbjct: 241 RDLQREVGNGSNSGESSLRNEEQ-QEQDEI--------------QPVRRSVSLDSLSALR 300

Query: 301 ISSVFAGFNEKTDCEAEIEQRNGNG-SKKSWQRRGNCSAAMNRSVSCSGKFLMRSSTQRS 354
           I S            A+I  RNGN   KKS Q R N SA MNRSVSCSG+F+MR+STQ S
Sbjct: 301 IISDL----------ADIGMRNGNDLKKKSCQERVNSSAVMNRSVSCSGEFVMRNSTQSS 327

BLAST of Spg004104 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 6.5e-31
Identity = 100/259 (38.61%), Postives = 138/259 (53.28%), Query Frame = 0

Query: 35  CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALL-SFTFLLVLCYAV 94
           CL+    +  +   P PPP         P   P +LS LL  +I ++ S    L L + +
Sbjct: 12  CLQFKDLRFCFRQYPPPPP---------PPPPPRELSLLLPTSICVVGSIILFLFLVFFL 71

Query: 95  YVRFYSGRRRRRRRGPDAPPEATHQQEFLDE-EHGPVLD-HPIWYIRTIGLPPAVIDAIA 154
           Y+      ++RR       P  T+QQE  DE E     D H +W I T+GL  + I++I 
Sbjct: 72  YLHI---TQQRRISAASVTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSIT 131

Query: 155 VCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAP 214
           V  FK GEG+IDGTECSVCL+EF+EDE+LRLLPKCSHAFHL CIDTWL SH NCP+CRAP
Sbjct: 132 VVGFKKGEGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAP 191

Query: 215 VVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTNEEQEQERDEIHLPDDDSE 274
           V+     +  PP     +ET   +   +   E++     + +  + +    I LP   S+
Sbjct: 192 VL----LITEPP----HQET---ETNHQPDSESSNDLRGRQDSSRSRRNHNIFLPRAQSD 247

Query: 275 ER----VGDFQPMRRSVSL 287
                  G  + +RRS S+
Sbjct: 252 LANYCGSGRVENVRRSFSI 247

BLAST of Spg004104 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.2e-30
Identity = 122/368 (33.15%), Postives = 177/368 (48.10%), Query Frame = 0

Query: 29  DSCPSG-CLENCPKQCPYLSIPIPPPNY---DDLNPNSPSKSPHKLSTLLTVTIALLSFT 88
           + C  G C   CP+ C YL  P PPP++   DD + +S S SP     LL   I +L+  
Sbjct: 15  NDCSQGICNIYCPQWC-YLIFPPPPPSFFLDDDSSSSSSSFSP-----LLIALIGILTSA 74

Query: 89  FLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQQEFLDEEH-GPVLDHPIWYIRT--IG 148
            +LV  Y + +  Y  R  +           T   E L+  H G         I T   G
Sbjct: 75  LILVSYYTL-ISKYCHRHHQ-----------TSSSETLNLNHNGEGFFSSTQRISTNGDG 134

Query: 149 LPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRS 208
           L  ++I +I V K+KSG+G +DG++CSVCLSEF+E+E+LRLLPKC+HAFHLPCIDTWL+S
Sbjct: 135 LNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKS 194

Query: 209 HTNCPMCRAPVVA------------------------QSAAVAPPPPVAASEETQIGDLQ 268
           H+NCP+CRA V                          Q+ +V+      A  E+Q GD  
Sbjct: 195 HSNCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDF- 254

Query: 269 REVGDEANLGESSQTNEEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSV 328
               D   + E  +        R E+ LP++  E +  D  P+RRSVSL+S   + I+ V
Sbjct: 255 ----DSVVVIEDLEIGSRNSDARSELQLPEERRETKDEDSLPIRRSVSLNSGVVVSIADV 314

Query: 329 FAGFNEKT-------DCEAEIEQRNGNGS----KKSWQRRGNCSAAMNRSVSCSGKFLMR 355
                ++          +   E  +G+G      ++ QR G  S    RS+S +G+F+  
Sbjct: 315 LREIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLS-TGRFIFS 358

BLAST of Spg004104 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 4.2e-30
Identity = 110/345 (31.88%), Postives = 168/345 (48.70%), Query Frame = 0

Query: 31  CPSGCLENCPKQCPYLSI--------------PIPPPNYDDLN-PNSPSKSPHKLSTLLT 90
           C   C  NC  +  Y +I              P P P+   +  P+  S S     +++T
Sbjct: 28  CDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISIIT 87

Query: 91  VTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRG----PDAPPEATHQQEFLDEEHGPVLD 150
           +T A+L+   +L+  + +  +F+S    R  +G     +   +   ++EF D E    +D
Sbjct: 88  ITGAVLA---ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ---VD 147

Query: 151 HPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFH 210
           HPIW IRT GL  ++I++I +C +K G+GLI+ T+C VCL+EF+EDE+LRLLPKC+HAFH
Sbjct: 148 HPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFH 207

Query: 211 LPCIDTWLRSHTNCPMCRAPVV-------AQSAAVAPPPPVAASEETQIGDLQREVGDEA 270
           + CIDTWL SHTNCP+CRA +          S  V   P  + S     G  + + G+  
Sbjct: 208 ISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGSHLENDGVDEEDHGEIE 267

Query: 271 NLGESSQTNEEQEQERDEIHLPDDD-------SEERVGDFQPMRRSVSLDSLSAL----- 330
           N  +S     +    R EI+  D D       ++ERV   +        DS+++L     
Sbjct: 268 NRVDSDFKESDDSDIRIEIYRFDSDGDGSETETKERVRVLKECMDPNGGDSVNSLSHTKT 327

Query: 331 RISSV-FAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCSAAMNRS 337
            + SV F G + +   E E  + NG       +  G  S  + RS
Sbjct: 328 HVESVDFPGKSCENQSE-EFTRHNGEDEASCSEENGGGSNQLRRS 365

BLAST of Spg004104 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 3.7e-26
Identity = 88/260 (33.85%), Postives = 121/260 (46.54%), Query Frame = 0

Query: 27  PSDSCPSG-CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTF 86
           P+  C  G C   CP+ C Y   P PPP +D    ++   S    S L+   I +L+  F
Sbjct: 13  PTRDCSQGLCSMYCPQWC-YFIFP-PPPPFDVAGTSADDSSGPVFSPLVIAIIGVLASAF 72

Query: 87  LLVLCYAVYVRFYSGRRRRRRR-----------GPDAPPEATHQQEFLDEEHGPVLDHPI 146
           LLV  Y    ++       R R           G  A     H        HG    H  
Sbjct: 73  LLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRH-------GHGQSRSHES 132

Query: 147 WYIR-TIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLP 206
           W +    GL   +I+ I VCK++ G+G +  T+CSVCL EF + E+LRLLP+CSHAFH  
Sbjct: 133 WNVSPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQ 192

Query: 207 CIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTN 266
           CIDTWL+SH+NCP+CRA +   +  +A P P   +     GD   EV     +       
Sbjct: 193 CIDTWLKSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGDNTHEV--VVVMDGLENLC 252

Query: 267 EEQEQERDEIHLPDDDSEER 274
           EEQ++        DDD + +
Sbjct: 253 EEQQEAVSRASTADDDHDAK 261

BLAST of Spg004104 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 3.5e-24
Identity = 95/275 (34.55%), Postives = 135/275 (49.09%), Query Frame = 0

Query: 31  CPSG-CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVL 90
           C  G C   CP+ C Y+  P PP  Y D   +S S      S LL   I +L+  F+LV 
Sbjct: 20  CSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSD---FSPLLIALIGILASAFILVS 79

Query: 91  CYAVYVRFYSGRRRRRRRGPDA------PPEATHQQEFLDEEHGPVLDHPIWYIRTI--- 150
            Y + +  Y  RRR       A        + T Q    +  +G    +     +TI   
Sbjct: 80  YYTL-ISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPN-----QTIGGG 139

Query: 151 ---GLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDT 210
              GL  ++I +I V K++  +G ++ ++CSVCLSEFQE+E+LRLLPKC+HAFH+PCIDT
Sbjct: 140 GGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDT 199

Query: 211 WLRSHTNCPMCRAPVVAQSAA----VAPPPPVAASEETQIGDLQREVGDEANLGESSQTN 270
           WL+SH+NCP+CRA +V  SA     +     V  +     GD    V    NL   +  +
Sbjct: 200 WLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVV---VNLDLENSRS 259

Query: 271 EEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDS 289
             +         P +  + R G+    RRS SL+S
Sbjct: 260 RNETVNEGSTPKPPEMQDSRDGE---ERRSASLNS 278

BLAST of Spg004104 vs. ExPASy TrEMBL
Match: A0A5D3C4M8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001880 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.8e-131
Identity = 266/359 (74.09%), Postives = 286/359 (79.67%), Query Frame = 0

Query: 1   MDFL-SRKLLS---DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYD 60
           MDFL SRKLLS   +DD+LKK C D C  + S SCPS CLE C   C Y+ IPIPPP+ D
Sbjct: 1   MDFLSSRKLLSSDDEDDDLKKLCKD-CFDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDAD 60

Query: 61  DLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT 120
           D+N  S +KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR R     PPE  
Sbjct: 61  DINQTSSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETH 120

Query: 121 HQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQE 180
           HQ EFLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+E
Sbjct: 121 HQHEFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEE 180

Query: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ-SAAVAPPPPVAASEETQIGD 240
           DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ SAA A PPP     ETQIG+
Sbjct: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGE 240

Query: 241 LQREVGDEANLGESSQTNE---EQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
           LQRE GD ANLGESS  NE   EQ+ E +E H  D+D EER  D QP+RRSVSLDSLSAL
Sbjct: 241 LQREEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSAL 300

Query: 301 RISSVFAGFNEKTDCEAEIEQRNGNGSKKSWQRRG-NCSAAMNRSVSCSGKFLMRSSTQ 351
           RISSVFA  NEK D +AE  QR G   KKS Q RG + +++MNRSVSCSGKFLMRSSTQ
Sbjct: 301 RISSVFANVNEKLDSDAETIQRKG---KKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQ 354

BLAST of Spg004104 vs. ExPASy TrEMBL
Match: A0A1S3BZS6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495376 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.8e-131
Identity = 266/359 (74.09%), Postives = 286/359 (79.67%), Query Frame = 0

Query: 1   MDFL-SRKLLS---DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYD 60
           MDFL SRKLLS   +DD+LKK C D C  + S SCPS CLE C   C Y+ IPIPPP+ D
Sbjct: 1   MDFLSSRKLLSSDDEDDDLKKLCKD-CFDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDAD 60

Query: 61  DLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT 120
           D+N  S +KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR R     PPE  
Sbjct: 61  DINQTSSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETH 120

Query: 121 HQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQE 180
           HQ EFLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+E
Sbjct: 121 HQHEFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEE 180

Query: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ-SAAVAPPPPVAASEETQIGD 240
           DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ SAA A PPP     ETQIG+
Sbjct: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGE 240

Query: 241 LQREVGDEANLGESSQTNE---EQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
           LQRE GD ANLGESS  NE   EQ+ E +E H  D+D EER  D QP+RRSVSLDSLSAL
Sbjct: 241 LQREEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSAL 300

Query: 301 RISSVFAGFNEKTDCEAEIEQRNGNGSKKSWQRRG-NCSAAMNRSVSCSGKFLMRSSTQ 351
           RISSVFA  NEK D +AE  QR G   KKS Q RG + +++MNRSVSCSGKFLMRSSTQ
Sbjct: 301 RISSVFANVNEKLDSDAETIQRKG---KKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQ 354

BLAST of Spg004104 vs. ExPASy TrEMBL
Match: A0A0A0K2P1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G066300 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 3.5e-128
Identity = 264/361 (73.13%), Postives = 283/361 (78.39%), Query Frame = 0

Query: 1   MDFL-SRKLLS-DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF  SRKLLS DDD+ K+FC D  D F SDSC S CLE C   C  ++IPI PP+ D +
Sbjct: 1   MDFFSSRKLLSEDDDDHKEFCKDCRDYF-SDSCSSDCLEKCRDYCYNINIPIRPPDADHI 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
           NP S  KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFY+GRRRR R     PPE  HQ
Sbjct: 61  NPTSSIKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYAGRRRRIRLPDPPPPETHHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
            +FLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+EDE
Sbjct: 121 HDFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS-AAVAPPPPVAASEETQIGDLQ 240
           TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS AA A PP +    ETQIG+LQ
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAAAASPPQLRPPAETQIGELQ 240

Query: 241 REVGDEANLGESSQTNEE--QEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRIS 300
           RE GD ANLGESS   EE  +EQE  E H  D+D EER  D QPMRRSVSLDSLSALRIS
Sbjct: 241 REEGDGANLGESSARTEEEQEEQEEQETHFADEDLEERSSDIQPMRRSVSLDSLSALRIS 300

Query: 301 SVFAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCS-AAMNRSVSCSGKFLMRSSTQRSNH 356
           S FA  NEK D EAE  QRNG   KKS Q RG+ S ++MNRSVSCS KFLMRSSTQ +  
Sbjct: 301 SDFANVNEKMDSEAETIQRNG---KKSSQGRGHSSTSSMNRSVSCSAKFLMRSSTQINTE 357

BLAST of Spg004104 vs. ExPASy TrEMBL
Match: A0A6J1EI25 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434557 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 3.9e-119
Identity = 249/363 (68.60%), Postives = 271/363 (74.66%), Query Frame = 0

Query: 1   MDFLSRKLLS--DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDFLSRKLLS  DDD+LKK C   C     DSCPS CLE CP  C   SI IPPP YD  
Sbjct: 1   MDFLSRKLLSDDDDDDLKKLCLKTCPNNSPDSCPSDCLEKCPNLCSRGSISIPPPYYD-- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
                 KSPHKLS  +T TIA LSFTFLLV+CY VYVRFYSGRRRR +R PD PP  THQ
Sbjct: 61  ------KSPHKLSNWVTATIAFLSFTFLLVICYTVYVRFYSGRRRRHQR-PDPPPPTTHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
           +EFLDEEHGPVLDHPIWYIRTIGLPPAVI+AIA+CKFK+GEGLIDGTECSVCLSEF+EDE
Sbjct: 121 EEFLDEEHGPVLDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVA-----PPPPVAASEETQI 240
           TLRLLPKCSHAFHLPCIDTWL+SHTNCPMCRAPVVAQS AV      PPPP+  SEETQ+
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQM 240

Query: 241 GDLQREVGDEANLGESSQTNEEQ--EQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSA 300
            DLQREVG+ +N GESS  NEEQ  +QE+DEI              Q +RRSVSLDSLSA
Sbjct: 241 RDLQREVGNGSNSGESSLRNEEQQEQQEQDEI--------------QAVRRSVSLDSLSA 300

Query: 301 LRISSVFAGFNEKTDCEAEIEQRNGNG-SKKSWQRRGNCSAAMNRSVSCSGKFLMRSSTQ 354
           LRI S            A+I  RNGN   KKS Q R N SAAMNRSVSCSG+F+MR+STQ
Sbjct: 301 LRIISDL----------ADIGMRNGNDLKKKSSQERVNSSAAMNRSVSCSGEFVMRNSTQ 330

BLAST of Spg004104 vs. ExPASy TrEMBL
Match: A0A6J1JNI4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111486075 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.8e-117
Identity = 246/362 (67.96%), Postives = 267/362 (73.76%), Query Frame = 0

Query: 1   MDFLSRKLL---SDDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDD 60
           MDFLSRKLL    DDD+ K+ C   C     D+CPS CLE CP  C    I IPPP YD 
Sbjct: 1   MDFLSRKLLPDDDDDDDFKELCLKTCPNNSPDNCPSDCLEKCPNLCSRGPISIPPPYYD- 60

Query: 61  LNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATH 120
                  KSPHKL   +T TIALLSFTFLLVLCY VYVRFYSGRRRRR+R PD PP  TH
Sbjct: 61  -------KSPHKLPNWVTATIALLSFTFLLVLCYTVYVRFYSGRRRRRQR-PDPPPPTTH 120

Query: 121 QQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQED 180
           Q+EFLDEEHGPVLDHPIWYIRTIGLPPAVI+AIA+CKFK+GEGLIDGTECSVCLSEF+ED
Sbjct: 121 QEEFLDEEHGPVLDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEED 180

Query: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVA-----PPPPVAASEETQ 240
           ETLRLLPKCSHAFHLPCIDTWL+SHTNCPMCRAPVVAQS AV      PPPP+  SEETQ
Sbjct: 181 ETLRLLPKCSHAFHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQ 240

Query: 241 IGDLQREVGDEANLGESSQTNEEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
           + DLQ EVG+ +N GESS  NEEQ QE+DEI              Q +RRSVSLDSLSAL
Sbjct: 241 MRDLQMEVGNGSNSGESSLRNEEQ-QEQDEI--------------QAVRRSVSLDSLSAL 300

Query: 301 RISSVFAGFNEKTDCEAEIEQRNGNG-SKKSWQRRGNCSAAMNRSVSCSGKFLMRSSTQR 354
           RI S            AEI  RNGN   KKS Q R N SAAMNRSVSCSG+F+MR+STQ 
Sbjct: 301 RIISDL----------AEIGMRNGNDLKKKSCQERVNSSAAMNRSVSCSGEFVMRNSTQS 328

BLAST of Spg004104 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 3.7e-45
Identity = 133/350 (38.00%), Postives = 185/350 (52.86%), Query Frame = 0

Query: 1   MDFLSRKLLSDDDNLKKFCDDYC------DRFPSDSCPSGCLENCPKQCPYLSIPIPPPN 60
           M FL R LL D    + F D  C      +      C S CL+ CP  C  + I      
Sbjct: 54  MRFLHRFLLEDMPK-ETFSDSLCKNNCIQELKTLGYCSSYCLDECPSICEIVVI------ 113

Query: 61  YDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPE 120
              L+P +       L  +L ++ + +  T  L+    +  +FY  RRR          E
Sbjct: 114 -SGLSPPAEIHHDDNLKRILIISASSIITTLFLLTLLVLCFKFYYRRRRSITTSRRWSME 173

Query: 121 ATHQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF 180
                +F D     ++DHPIW+IRTIGL P VI +I VC++   +G+++GT+CSVCLSEF
Sbjct: 174 EARNWDF-DGPAPVIVDHPIWHIRTIGLNPTVISSIKVCQYSKKDGVVEGTDCSVCLSEF 233

Query: 181 QEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIG 240
           +E+ETLRLLPKC HAFHL CIDTWLRSHTNCP+CRAP+V  +  +         EE  + 
Sbjct: 234 EEEETLRLLPKCKHAFHLYCIDTWLRSHTNCPLCRAPIVEANTMI--DDHSEGLEEISV- 293

Query: 241 DLQREVGDEANLGESSQTNEEQEQERDEI--HLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
            +  E GD+        T+EE E+ERD    ++  D  E +    Q +RRSVSLDSLS L
Sbjct: 294 MIPEENGDD--------TDEEIEEERDGFVSNISRDHGESQQ---QRVRRSVSLDSLSGL 353

Query: 301 RISSVFAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCSAAMNRSVSCSGK 343
           R+S V  G  ++     ++++ N  GS +        S+ + RS+S SGK
Sbjct: 354 RVSEVVVGREKE-----KLKRGNNIGSGR--------SSLLKRSISYSGK 367

BLAST of Spg004104 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 4.6e-32
Identity = 100/259 (38.61%), Postives = 138/259 (53.28%), Query Frame = 0

Query: 35  CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALL-SFTFLLVLCYAV 94
           CL+    +  +   P PPP         P   P +LS LL  +I ++ S    L L + +
Sbjct: 12  CLQFKDLRFCFRQYPPPPP---------PPPPPRELSLLLPTSICVVGSIILFLFLVFFL 71

Query: 95  YVRFYSGRRRRRRRGPDAPPEATHQQEFLDE-EHGPVLD-HPIWYIRTIGLPPAVIDAIA 154
           Y+      ++RR       P  T+QQE  DE E     D H +W I T+GL  + I++I 
Sbjct: 72  YLHI---TQQRRISAASVTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSIT 131

Query: 155 VCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAP 214
           V  FK GEG+IDGTECSVCL+EF+EDE+LRLLPKCSHAFHL CIDTWL SH NCP+CRAP
Sbjct: 132 VVGFKKGEGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAP 191

Query: 215 VVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTNEEQEQERDEIHLPDDDSE 274
           V+     +  PP     +ET   +   +   E++     + +  + +    I LP   S+
Sbjct: 192 VL----LITEPP----HQET---ETNHQPDSESSNDLRGRQDSSRSRRNHNIFLPRAQSD 247

Query: 275 ER----VGDFQPMRRSVSL 287
                  G  + +RRS S+
Sbjct: 252 LANYCGSGRVENVRRSFSI 247

BLAST of Spg004104 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 134.0 bits (336), Expect = 2.3e-31
Identity = 122/368 (33.15%), Postives = 177/368 (48.10%), Query Frame = 0

Query: 29  DSCPSG-CLENCPKQCPYLSIPIPPPNY---DDLNPNSPSKSPHKLSTLLTVTIALLSFT 88
           + C  G C   CP+ C YL  P PPP++   DD + +S S SP     LL   I +L+  
Sbjct: 15  NDCSQGICNIYCPQWC-YLIFPPPPPSFFLDDDSSSSSSSFSP-----LLIALIGILTSA 74

Query: 89  FLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQQEFLDEEH-GPVLDHPIWYIRT--IG 148
            +LV  Y + +  Y  R  +           T   E L+  H G         I T   G
Sbjct: 75  LILVSYYTL-ISKYCHRHHQ-----------TSSSETLNLNHNGEGFFSSTQRISTNGDG 134

Query: 149 LPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRS 208
           L  ++I +I V K+KSG+G +DG++CSVCLSEF+E+E+LRLLPKC+HAFHLPCIDTWL+S
Sbjct: 135 LNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKS 194

Query: 209 HTNCPMCRAPVVA------------------------QSAAVAPPPPVAASEETQIGDLQ 268
           H+NCP+CRA V                          Q+ +V+      A  E+Q GD  
Sbjct: 195 HSNCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDF- 254

Query: 269 REVGDEANLGESSQTNEEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSV 328
               D   + E  +        R E+ LP++  E +  D  P+RRSVSL+S   + I+ V
Sbjct: 255 ----DSVVVIEDLEIGSRNSDARSELQLPEERRETKDEDSLPIRRSVSLNSGVVVSIADV 314

Query: 329 FAGFNEKT-------DCEAEIEQRNGNGS----KKSWQRRGNCSAAMNRSVSCSGKFLMR 355
                ++          +   E  +G+G      ++ QR G  S    RS+S +G+F+  
Sbjct: 315 LREIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLS-TGRFIFS 358

BLAST of Spg004104 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 3.0e-31
Identity = 110/345 (31.88%), Postives = 168/345 (48.70%), Query Frame = 0

Query: 31  CPSGCLENCPKQCPYLSI--------------PIPPPNYDDLN-PNSPSKSPHKLSTLLT 90
           C   C  NC  +  Y +I              P P P+   +  P+  S S     +++T
Sbjct: 28  CDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISIIT 87

Query: 91  VTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRG----PDAPPEATHQQEFLDEEHGPVLD 150
           +T A+L+   +L+  + +  +F+S    R  +G     +   +   ++EF D E    +D
Sbjct: 88  ITGAVLA---ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ---VD 147

Query: 151 HPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFH 210
           HPIW IRT GL  ++I++I +C +K G+GLI+ T+C VCL+EF+EDE+LRLLPKC+HAFH
Sbjct: 148 HPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFH 207

Query: 211 LPCIDTWLRSHTNCPMCRAPVV-------AQSAAVAPPPPVAASEETQIGDLQREVGDEA 270
           + CIDTWL SHTNCP+CRA +          S  V   P  + S     G  + + G+  
Sbjct: 208 ISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGSHLENDGVDEEDHGEIE 267

Query: 271 NLGESSQTNEEQEQERDEIHLPDDD-------SEERVGDFQPMRRSVSLDSLSAL----- 330
           N  +S     +    R EI+  D D       ++ERV   +        DS+++L     
Sbjct: 268 NRVDSDFKESDDSDIRIEIYRFDSDGDGSETETKERVRVLKECMDPNGGDSVNSLSHTKT 327

Query: 331 RISSV-FAGFNEKTDCEAEIEQRNGNGSKKSWQRRGNCSAAMNRS 337
            + SV F G + +   E E  + NG       +  G  S  + RS
Sbjct: 328 HVESVDFPGKSCENQSE-EFTRHNGEDEASCSEENGGGSNQLRRS 365

BLAST of Spg004104 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 2.5e-25
Identity = 95/275 (34.55%), Postives = 135/275 (49.09%), Query Frame = 0

Query: 31  CPSG-CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVL 90
           C  G C   CP+ C Y+  P PP  Y D   +S S      S LL   I +L+  F+LV 
Sbjct: 20  CSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSD---FSPLLIALIGILASAFILVS 79

Query: 91  CYAVYVRFYSGRRRRRRRGPDA------PPEATHQQEFLDEEHGPVLDHPIWYIRTI--- 150
            Y + +  Y  RRR       A        + T Q    +  +G    +     +TI   
Sbjct: 80  YYTL-ISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPN-----QTIGGG 139

Query: 151 ---GLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDT 210
              GL  ++I +I V K++  +G ++ ++CSVCLSEFQE+E+LRLLPKC+HAFH+PCIDT
Sbjct: 140 GGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDT 199

Query: 211 WLRSHTNCPMCRAPVVAQSAA----VAPPPPVAASEETQIGDLQREVGDEANLGESSQTN 270
           WL+SH+NCP+CRA +V  SA     +     V  +     GD    V    NL   +  +
Sbjct: 200 WLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVV---VNLDLENSRS 259

Query: 271 EEQEQERDEIHLPDDDSEERVGDFQPMRRSVSLDS 289
             +         P +  + R G+    RRS SL+S
Sbjct: 260 RNETVNEGSTPKPPEMQDSRDGE---ERRSASLNS 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455131.11.2e-13074.09PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0031432.1 E... [more]
XP_038888113.11.3e-12973.37E3 ubiquitin-protein ligase RING1-like isoform X2 [Benincasa hispida][more]
XP_004137067.17.2e-12873.13RING-H2 finger protein ATL54 [Cucumis sativus] >KGN43773.1 hypothetical protein ... [more]
XP_038888112.12.0e-12273.00E3 ubiquitin-protein ligase RING1-like isoform X1 [Benincasa hispida][more]
KAG7022535.11.5e-12069.25RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
Q9LX936.5e-3138.61E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ... [more]
Q9LF643.2e-3033.15RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q8LFY84.2e-3031.88RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Q7XLY83.7e-2633.85E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SRQ83.5e-2434.55RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3C4M85.8e-13174.09RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BZS65.8e-13174.09RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495376 PE=3 ... [more]
A0A0A0K2P13.5e-12873.13RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G066300 PE... [more]
A0A6J1EI253.9e-11968.60RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434557... [more]
A0A6J1JNI42.8e-11767.96RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111486075 P... [more]
Match NameE-valueIdentityDescription
AT4G33565.13.7e-4538.00RING/U-box superfamily protein [more]
AT5G10380.14.6e-3238.61RING/U-box superfamily protein [more]
AT5G17600.12.3e-3133.15RING/U-box superfamily protein [more]
AT1G72220.13.0e-3131.88RING/U-box superfamily protein [more]
AT3G03550.12.5e-2534.55RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 167..208
e-value: 1.8E-7
score: 40.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 166..209
e-value: 4.3E-13
score: 49.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 167..209
score: 12.872856
NoneNo IPR availableSMARTSM01197FANCL_C_2coord: 163..216
e-value: 0.0084
score: -1.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 243..279
NoneNo IPR availablePANTHERPTHR45676:SF126RING-H2 FINGER PROTEIN ATL54coord: 28..346
NoneNo IPR availableCDDcd16461RING-H2_EL5_likecoord: 166..209
e-value: 1.82994E-15
score: 67.7203
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 161..215
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 144..218
e-value: 1.3E-21
score: 77.6
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 28..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg004104.1Spg004104.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane