Spg004048 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg004048
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCYSTM domain-containing protein
Locationscaffold4: 1565948 .. 1567187 (-)
RNA-Seq ExpressionSpg004048
SyntenySpg004048
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAAAAAACCATTTATTTCTTGAGCTTTCTCATAAAGTTTAGTGATTTAAATACACCCTTTGTTCTTCTTCTTCTTTCTTCTCTTGCATTGTTCAAGCTTGTGTTGGAAGAAGATATGAGTGACCCCAAATATGCCTATCCCTACCCAGCTCCTCAAGGTATTTTTTGCGTATCATCTATCGTTAATGTCTTAACCGTTGAGTTATGTTCAAATTGATTTCTCTATTTGAGTTTTCGTTTGATTTGGTCTTCTGGGTGTTGTGACTTTTTTTCACAGGAGGTTATTATCAAGGACCGCCGGTGATGGCACCGCCGCAGTACGCCGCGCCGCCGCCCAAAAGACAGCCAGGCTTCTTGGAGGGATGGTATGTCAAAGCTTATTCTTTTATGTTGATAAGAATGGAACTTATATCAAAAGTGAAGGCTAGAATTGAGGGTGTTGGGAAAATAAAACATGGAACAATTAGGAGTAGATGAAATAATCTAAGGTATATCAAAAGAAAAACGACACCGATATTTATACTGGTTCATCAATTGTAAAACCATGTCTAATTTATGCTAACATTCTAGATTTATAGACACTCTCTTCACACTTCTCCCCTCTATCTCTTTCTCACACACAAGCCCTTAGAAAATAGGTCGTTATTATAAAGAATATCACGTGCGGGCGTCTAGGCGCTACGAATAGAGCATTCTGCCATCAAATTGGCGCCTCGACACCCTCCTCTCGAGCTCTTAAACATTTCAAAGCAACAGCGCGGGGCGCCTAGGGGCTTCGAAATTTGAATCACACACCTTTTTTCTTTTGTCATCTCTGTTTTTGCCTTCATATGCCTAACAAAAGGTTTAAATTTTTGTGTTGCAGCCTTGCAGCCCTGTGCTGCTGCTGTCTCCTTGATGAGTGTTGCTGTGACCCTTCCATTATCTTTGTTGCTTGATCTGAGCTTTGATTTTGCCTTACACTTCTCTTTTTCTTTGGGAGTGGTTTGATTTTGTGTAAACTTGGCAAGAAGATTAAATGGTTGTATAGAATAATAAAGCTTTGTTTTCTGTTGTAAAGTATAGAGTTTACACATTTGATGGTAACTTTATGGGTTAGCGGAGTTACAAATTTAGTCCATAAACTTTTGATTATATTGTTGCGTTTATTTAGGTTTTAAATTCGAATTATGATTCTATATGTATCTGTTGTTAACCTCTTGTTGTAAGCTAAATGATAATATAA

mRNA sequence

ATGAGTGACCCCAAATATGCCTATCCCTACCCAGCTCCTCAAGGAGGTTATTATCAAGGACCGCCGGTGATGGCACCGCCGCAGTACGCCGCGCCGCCGCCCAAAAGACAGCCAGGCTTCTTGGAGGGATGCCTTGCAGCCCTGTGCTGCTGCTGTCTCCTTGATGAGTGTTGCTGTGACCCTTCCATTATCTTTGTTGCTTGA

Coding sequence (CDS)

ATGAGTGACCCCAAATATGCCTATCCCTACCCAGCTCCTCAAGGAGGTTATTATCAAGGACCGCCGGTGATGGCACCGCCGCAGTACGCCGCGCCGCCGCCCAAAAGACAGCCAGGCTTCTTGGAGGGATGCCTTGCAGCCCTGTGCTGCTGCTGTCTCCTTGATGAGTGTTGCTGTGACCCTTCCATTATCTTTGTTGCTTGA

Protein sequence

MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCDPSIIFVA
Homology
BLAST of Spg004048 vs. NCBI nr
Match: KAG6571885.1 (hypothetical protein SDJN03_28613, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011569.1 hypothetical protein SDJN02_26475 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 137.5 bits (345), Expect = 4.2e-29
Identity = 62/66 (93.94%), Postives = 63/66 (95.45%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYAYPYP P GGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD
Sbjct: 1  MSDPKYAYPYPPPPGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60

Query: 61 PSIIFV 67
          PSI+ V
Sbjct: 61 PSILLV 66

BLAST of Spg004048 vs. NCBI nr
Match: KAF9609677.1 (hypothetical protein IFM89_017856 [Coptis chinensis])

HSP 1 Score: 134.8 bits (338), Expect = 2.7e-28
Identity = 61/66 (92.42%), Postives = 63/66 (95.45%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          M+DPKYAYPYP P  GYYQGPPVMAPPQYAAPPP+RQPGFLEGCLAALCCCCLLDECCCD
Sbjct: 1  MNDPKYAYPYPYPAQGYYQGPPVMAPPQYAAPPPRRQPGFLEGCLAALCCCCLLDECCCD 60

Query: 61 PSIIFV 67
          PSIIFV
Sbjct: 61 PSIIFV 66

BLAST of Spg004048 vs. NCBI nr
Match: EEF35140.1 (conserved hypothetical protein [Ricinus communis])

HSP 1 Score: 131.0 bits (328), Expect = 4.0e-27
Identity = 61/67 (91.04%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYAYPYPA   GYYQGPPVMAPPQYAAPPP+RQPGFLEGCLAALCCCCL+DECCCD
Sbjct: 1  MSDPKYAYPYPAQ--GYYQGPPVMAPPQYAAPPPRRQPGFLEGCLAALCCCCLIDECCCD 60

Query: 61 PSIIFVA 68
          PSIIFV+
Sbjct: 61 PSIIFVS 65

BLAST of Spg004048 vs. NCBI nr
Match: XP_010254642.1 (PREDICTED: uncharacterized protein LOC104595564 [Nelumbo nucifera])

HSP 1 Score: 130.6 bits (327), Expect = 5.2e-27
Identity = 61/67 (91.04%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1   MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
           MSDPKYAYPYPA   GYYQGPPVMAPPQYAAPPP+R+PGFLEGCLAALCCCCLLDECCCD
Sbjct: 92  MSDPKYAYPYPAQ--GYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLLDECCCD 151

Query: 61  PSIIFVA 68
           PSIIFV+
Sbjct: 152 PSIIFVS 156

BLAST of Spg004048 vs. NCBI nr
Match: DAD40851.1 (TPA_asm: hypothetical protein HUJ06_015174 [Nelumbo nucifera])

HSP 1 Score: 130.6 bits (327), Expect = 5.2e-27
Identity = 61/67 (91.04%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYAYPYPA   GYYQGPPVMAPPQYAAPPP+R+PGFLEGCLAALCCCCLLDECCCD
Sbjct: 1  MSDPKYAYPYPAQ--GYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLLDECCCD 60

Query: 61 PSIIFVA 68
          PSIIFV+
Sbjct: 61 PSIIFVS 65

BLAST of Spg004048 vs. ExPASy Swiss-Prot
Match: Q8S8M0 (Cysteine-rich and transmembrane domain-containing protein WIH2 OS=Arabidopsis thaliana OX=3702 GN=WIH2 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.2e-07
Identity = 35/60 (58.33%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 4  PKYAYPYPA-PQGGY-YQGPPVMAPPQYAAPPPKRQ----PGFLEGCLAALCCCCLLDEC 58
          P+  YP    PQ GY  QG P    PQY  PP  +Q    PGFLEGCLAALCCCCLLD C
Sbjct: 38 PQQGYPPQGYPQQGYPQQGYPPPYAPQYPPPPQHQQQQSSPGFLEGCLAALCCCCLLDAC 97

BLAST of Spg004048 vs. ExPASy Swiss-Prot
Match: Q9FJW3 (Cysteine-rich and transmembrane domain-containing protein WIH1 OS=Arabidopsis thaliana OX=3702 GN=WIH1 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.1e-07
Identity = 36/63 (57.14%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 4  PKYAYP---YPA---PQGGYYQGPPVMA--PPQYA-APPPKRQPGFLEGCLAALCCCCLL 58
          PK  YP   YP    P  GY QG P     PPQY+ AP  K+  G LEGCLAALCCCCLL
Sbjct: 19 PKDGYPPAGYPPAGYPPPGYAQGYPAQGYPPPQYSQAPQQKQNAGMLEGCLAALCCCCLL 78

BLAST of Spg004048 vs. ExPASy Swiss-Prot
Match: Q8LCL8 (Cysteine-rich and transmembrane domain-containing protein B OS=Arabidopsis thaliana OX=3702 GN=At3g57160 PE=3 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 31/62 (50.00%), Postives = 35/62 (56.45%), Query Frame = 0

Query: 4   PKYAYP---YPAPQGGYYQGPPVMAPPQYAAPPPKRQ-----PGFLEGCLAALCCCCLLD 58
           P   YP   YP PQG   QG P    PQ   PP ++Q     PG LEGC+AALCC C+LD
Sbjct: 40  PPQGYPQQGYP-PQGYPPQGYPEQGYPQQGYPPQQQQQQKHSPGMLEGCIAALCCYCVLD 99

BLAST of Spg004048 vs. ExPASy TrEMBL
Match: B9SMJ5 (CYSTM domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_0230320 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.9e-27
Identity = 61/67 (91.04%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYAYPYPA   GYYQGPPVMAPPQYAAPPP+RQPGFLEGCLAALCCCCL+DECCCD
Sbjct: 1  MSDPKYAYPYPAQ--GYYQGPPVMAPPQYAAPPPRRQPGFLEGCLAALCCCCLIDECCCD 60

Query: 61 PSIIFVA 68
          PSIIFV+
Sbjct: 61 PSIIFVS 65

BLAST of Spg004048 vs. ExPASy TrEMBL
Match: A0A1U8A0R0 (uncharacterized protein LOC104595564 OS=Nelumbo nucifera OX=4432 GN=LOC104595564 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.5e-27
Identity = 61/67 (91.04%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1   MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
           MSDPKYAYPYPA   GYYQGPPVMAPPQYAAPPP+R+PGFLEGCLAALCCCCLLDECCCD
Sbjct: 92  MSDPKYAYPYPAQ--GYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLLDECCCD 151

Query: 61  PSIIFVA 68
           PSIIFV+
Sbjct: 152 PSIIFVS 156

BLAST of Spg004048 vs. ExPASy TrEMBL
Match: A0A314Y8T5 (CYSTM domain-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_05607 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.3e-27
Identity = 58/66 (87.88%), Postives = 63/66 (95.45%), Query Frame = 0

Query: 1  MSDPKYAYPYPAP-QGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCC 60
          MSDPKY YPYPA  QGGYYQGPPVMAPPQYAAPPP+R+PGFLEGCLAALCCCCL+DECCC
Sbjct: 1  MSDPKYGYPYPAQGQGGYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCC 60

Query: 61 DPSIIF 66
          DPS++F
Sbjct: 61 DPSVLF 66

BLAST of Spg004048 vs. ExPASy TrEMBL
Match: A0A2P5FH35 (Cysteine-rich transmembrane CYSTM domain containing protein OS=Trema orientale OX=63057 GN=TorRG33x02_071570 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.3e-27
Identity = 60/67 (89.55%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYAYPYPA   GYYQGPPVMAPPQYAAPPP+R+PGFLEGCLAALCCCCLLDECCCD
Sbjct: 1  MSDPKYAYPYPAQ--GYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLLDECCCD 60

Query: 61 PSIIFVA 68
          PSIIF++
Sbjct: 61 PSIIFIS 65

BLAST of Spg004048 vs. ExPASy TrEMBL
Match: A0A2C9UQL9 (CYSTM domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_13G104200 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.3e-27
Identity = 60/67 (89.55%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQPGFLEGCLAALCCCCLLDECCCD 60
          MSDPKYAYPYPA   GYYQGPPVMAPPQYAAPPP+R+PGFLEGCLAALCCCCL+DECCCD
Sbjct: 1  MSDPKYAYPYPAQ--GYYQGPPVMAPPQYAAPPPRREPGFLEGCLAALCCCCLIDECCCD 60

Query: 61 PSIIFVA 68
          PSIIFV+
Sbjct: 61 PSIIFVS 65

BLAST of Spg004048 vs. TAIR 10
Match: AT4G33660.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 94.4 bits (233), Expect = 3.8e-20
Identity = 51/74 (68.92%), Postives = 54/74 (72.97%), Query Frame = 0

Query: 1  MSDPKYAYPYPAPQGGYYQG--PPVMAPPQY--------AAPPPKRQPGFLEGCLAALCC 60
          MSDPKYAYPYPAP G Y QG  PPV  PPQY          PPP R+ GFLEG LAALCC
Sbjct: 1  MSDPKYAYPYPAP-GNYPQGPPPPVGVPPQYYPPPPPPPPPPPPPRKVGFLEGLLAALCC 60

Query: 61 CCLLDECCCDPSII 65
          CCL+DECCCDP+II
Sbjct: 61 CCLVDECCCDPTII 73

BLAST of Spg004048 vs. TAIR 10
Match: AT2G41420.1 (proline-rich family protein )

HSP 1 Score: 56.6 bits (135), Expect = 8.8e-09
Identity = 35/60 (58.33%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 4  PKYAYPYPA-PQGGY-YQGPPVMAPPQYAAPPPKRQ----PGFLEGCLAALCCCCLLDEC 58
          P+  YP    PQ GY  QG P    PQY  PP  +Q    PGFLEGCLAALCCCCLLD C
Sbjct: 38 PQQGYPPQGYPQQGYPQQGYPPPYAPQYPPPPQHQQQQSSPGFLEGCLAALCCCCLLDAC 97

BLAST of Spg004048 vs. TAIR 10
Match: AT5G67600.1 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G49845.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 55.8 bits (133), Expect = 1.5e-08
Identity = 36/63 (57.14%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 4  PKYAYP---YPA---PQGGYYQGPPVMA--PPQYA-APPPKRQPGFLEGCLAALCCCCLL 58
          PK  YP   YP    P  GY QG P     PPQY+ AP  K+  G LEGCLAALCCCCLL
Sbjct: 19 PKDGYPPAGYPPAGYPPPGYAQGYPAQGYPPPQYSQAPQQKQNAGMLEGCLAALCCCCLL 78

BLAST of Spg004048 vs. TAIR 10
Match: AT3G49845.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: root; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 5.4e-06
Identity = 29/60 (48.33%), Postives = 32/60 (53.33%), Query Frame = 0

Query: 4   PKYAYPYPAPQGGYYQGPPVMAPPQYAAPPPKRQP------GFLEGCLAALCCCCLLDEC 58
           P   YP   P    YQGPP   PP Y   PPK +       GF+EGCLA LCCC LL+ C
Sbjct: 67  PPPQYPQGHPPQYPYQGPP---PPHYGQAPPKNKKDKKDSGGFMEGCLAMLCCCVLLEAC 123

BLAST of Spg004048 vs. TAIR 10
Match: AT1G12810.1 (proline-rich family protein )

HSP 1 Score: 42.7 bits (99), Expect = 1.3e-04
Identity = 32/85 (37.65%), Postives = 35/85 (41.18%), Query Frame = 0

Query: 8   YPYPAPQGGY-----------YQGPPVMA--PPQYAAPPPKRQP---------------- 59
           YP P P GGY           YQG       P Q+  PPP   P                
Sbjct: 44  YPPPQPYGGYPPPSSRPYEGGYQGYFAGGGYPHQHHGPPPPPPPQNYDHCHHDHHHYQDS 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6571885.14.2e-2993.94hypothetical protein SDJN03_28613, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAF9609677.12.7e-2892.42hypothetical protein IFM89_017856 [Coptis chinensis][more]
EEF35140.14.0e-2791.04conserved hypothetical protein [Ricinus communis][more]
XP_010254642.15.2e-2791.04PREDICTED: uncharacterized protein LOC104595564 [Nelumbo nucifera][more]
DAD40851.15.2e-2791.04TPA_asm: hypothetical protein HUJ06_015174 [Nelumbo nucifera][more]
Match NameE-valueIdentityDescription
Q8S8M01.2e-0758.33Cysteine-rich and transmembrane domain-containing protein WIH2 OS=Arabidopsis th... [more]
Q9FJW32.1e-0757.14Cysteine-rich and transmembrane domain-containing protein WIH1 OS=Arabidopsis th... [more]
Q8LCL81.3e-0450.00Cysteine-rich and transmembrane domain-containing protein B OS=Arabidopsis thali... [more]
Match NameE-valueIdentityDescription
B9SMJ51.9e-2791.04CYSTM domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_0230320 PE=3... [more]
A0A1U8A0R02.5e-2791.04uncharacterized protein LOC104595564 OS=Nelumbo nucifera OX=4432 GN=LOC104595564... [more]
A0A314Y8T53.3e-2787.88CYSTM domain-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN... [more]
A0A2P5FH353.3e-2789.55Cysteine-rich transmembrane CYSTM domain containing protein OS=Trema orientale O... [more]
A0A2C9UQL94.3e-2789.55CYSTM domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_13G104200 ... [more]
Match NameE-valueIdentityDescription
AT4G33660.13.8e-2068.92unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -... [more]
AT2G41420.18.8e-0958.33proline-rich family protein [more]
AT5G67600.11.5e-0857.14unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 22 plant structures;... [more]
AT3G49845.15.4e-0648.33unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G12810.11.3e-0437.65proline-rich family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR028144Cysteine-rich transmembrane CYSTM domainPFAMPF12734CYSTMcoord: 12..58
e-value: 4.2E-15
score: 55.8
NoneNo IPR availablePANTHERPTHR31568:SF105CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN A-LIKEcoord: 1..66
IPR044850Cysteine-rich and transmembrane domain-containing protein WIH1/2-likePANTHERPTHR31568RCG49325, ISOFORM CRA_Acoord: 1..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg004048.1Spg004048.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane