Spg004009 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg004009 vs. NCBI nr
Match: XP_011659040.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 3428.6 bits (8889), Expect = 0.0e+00
Identity = 1773/1965 (90.23%), Postives = 1793/1965 (91.25%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPKQ+GPSPLPAYEPAFDWENERSM FGQRIPETPV                       
Sbjct: 121  NVPKQIGPSPLPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDSHH SGSSKLNF  GKE SGNGS+ H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSHHLSGSSKLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA+
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAI 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECLILFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1836

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1836

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of Spg004009 vs. NCBI nr
Match: XP_011659041.1 (guanine nucleotide exchange factor SPIKE 1 isoform X2 [Cucumis sativus] >KAE8646107.1 hypothetical protein Csa_015554 [Cucumis sativus])

HSP 1 Score: 3422.1 bits (8872), Expect = 0.0e+00
Identity = 1772/1965 (90.18%), Postives = 1792/1965 (91.20%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPK +GPSPLPAYEPAFDWENERSM FGQRIPETPV                       
Sbjct: 121  NVPK-IGPSPLPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDSHH SGSSKLNF  GKE SGNGS+ H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSHHLSGSSKLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA+
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAI 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECLILFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1835

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1835

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1835

BLAST of Spg004009 vs. NCBI nr
Match: XP_038888190.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 3414.8 bits (8853), Expect = 0.0e+00
Identity = 1770/1965 (90.08%), Postives = 1790/1965 (91.09%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETI PVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETISPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPKQ+GPSPLPAYEPAFDWENERSMIFGQRIPETPV                       
Sbjct: 121  NVPKQIGPSPLPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITG+SSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGTSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR VDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPVDSTFKKHPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SD HH SGSSKL+ S  KE SGNGS PH NAD+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDRHHVSGSSKLSCSVVKEFSGNGSSPHENADTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQ VLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQPVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA+
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAI 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGD PGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDVPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAF+ ALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFHNALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS+LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1836

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1836

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of Spg004009 vs. NCBI nr
Match: TYJ96782.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 3412.9 bits (8848), Expect = 0.0e+00
Identity = 1767/1965 (89.92%), Postives = 1786/1965 (90.89%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPKQ+GPSPLPAYEPAFDWENERSM FGQRIPETP                        
Sbjct: 121  NVPKQIGPSPLPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDS H S SSK NF  GKE SGNGS  H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSQHLSCSSKSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA 
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAT 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECL+LFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLVLFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1836

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1836

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of Spg004009 vs. NCBI nr
Match: XP_008447100.1 (PREDICTED: guanine nucleotide exchange factor SPIKE 1 isoform X1 [Cucumis melo])

HSP 1 Score: 3410.5 bits (8842), Expect = 0.0e+00
Identity = 1766/1965 (89.87%), Postives = 1786/1965 (90.89%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWP+LNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPYLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPKQ+GPSPLPAYEPAFDWENERSM FGQRIPETP                        
Sbjct: 121  NVPKQIGPSPLPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDS H S SSK NF  GKE SGNGS  H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSQHLSCSSKSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA 
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAT 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECL+LFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLVLFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1836

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1836

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of Spg004009 vs. ExPASy Swiss-Prot
Match: Q8SAB7 (Guanine nucleotide exchange factor SPIKE 1 OS=Arabidopsis thaliana OX=3702 GN=SPK1 PE=1 SV=1)

HSP 1 Score: 2798.8 bits (7254), Expect = 0.0e+00
Identity = 1460/1948 (74.95%), Postives = 1610/1948 (82.65%), Query Frame = 0

Query: 21   EENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQNQIYEGPDTDIETGNYAFQH 80
            +ENLEQWPHLN+LVQCY T+WVKD NKYGHYE I P SFQ QI+EGPDTD ET       
Sbjct: 28   DENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTET------- 87

Query: 81   GIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLSNVPKQLGPSPLPAYEPAFDW 140
               E+RL  AR      T E+DV S SGRP +   DP  S   K  G  PLPAYEPAFDW
Sbjct: 88   ---EIRLASARSA----TIEEDVASISGRPFS---DPGSS---KHFGQPPLPAYEPAFDW 147

Query: 141  ENERSMIFGQRIPETPV---------------------------------SQERREKLSE 200
            ENER+MIFGQR PE+P                                  +QER+EKLSE
Sbjct: 148  ENERAMIFGQRTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSE 207

Query: 201  DFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASVYSRKEPV 260
            DF+F+I P EMQD K+S E RG+FYL+APSASVCL IQLEK ATEEGGVT+SVYSRKEPV
Sbjct: 208  DFYFQIQPTEMQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPV 267

Query: 261  HLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSASPSSPLAPSITGSSSHE 320
            HL EREKQKLQVWS+IMPY+ESFAWA+V LFDN+    +  SASPSSPLAPS+T SSSH+
Sbjct: 268  HLTEREKQKLQVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHD 327

Query: 321  GVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPVKGVLRLE 380
            GV+EP  K+T DGK GYS GSSVVVEISNLNKVKE Y+E+++QDPK KVHKPVKGVLRLE
Sbjct: 328  GVYEPIAKITSDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLE 387

Query: 381  IEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNGSDSHHHSGSSKLNFSDG 440
            IEKH+  H D E++SE+GS+I+DS+D  DRL D T  K P++ S     +G SK N  D 
Sbjct: 388  IEKHRNGHGDFEDLSENGSIINDSLDPTDRLSDLTLMKCPSSSSGG-PRNGCSKWNSEDA 447

Query: 441  KELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLYVYPLTVSLSRKRNLFIR 500
            K++S N +   G  D N   +HAFDF    RNEPFL LFHCLYVYP+ V+LSRKRN FIR
Sbjct: 448  KDVSRNLTSSCGTPDLNC--YHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIR 507

Query: 501  IELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLPATWTPKH 560
            +ELR+DD+D R+QPLEA+YP E G SLQKW HTQVAVGAR A YHDEIK+SLPATWTP H
Sbjct: 508  VELRKDDTDIRKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSH 567

Query: 561  HLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASSDGRGGFAWVRLTECPGE 620
            HLLFTFF+VD+Q KLEAP+PV +GYASLPLST+                           
Sbjct: 568  HLLFTFFHVDLQTKLEAPRPVVVGYASLPLSTY--------------------------- 627

Query: 621  CLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLRSEISLPVMRELVPHYLQ 680
                       +H+                            RS+ISLPVMRELVPHYLQ
Sbjct: 628  -----------IHS----------------------------RSDISLPVMRELVPHYLQ 687

Query: 681  DTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAI 740
            ++ +ERLDYLEDGKNIFKLRLRLCSSLYP NER+RDF LEYDRHTL+T PPWGSELL+AI
Sbjct: 688  ESTKERLDYLEDGKNIFKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAI 747

Query: 741  NSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERNH 800
            NSLK+VDSTALLQFL+PILNMLLHLIGNGGETLQVAAFRAMV+I+TRVQQ S +D +RN 
Sbjct: 748  NSLKHVDSTALLQFLYPILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNR 807

Query: 801  FLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIV 860
            FLV YVDY+FDDFGG QPPVYPGL+TVWGSLARSKAKGYRVGPVYDDVL+MAWFFLELIV
Sbjct: 808  FLVTYVDYSFDDFGGNQPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIV 867

Query: 861  KSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIPPMQLKEGVFRCIMQLYD 920
            KSMALE+ RL+ H+LP G                     ED+PPMQLKE VFRCIMQL+D
Sbjct: 868  KSMALEQARLYDHNLPTG---------------------EDVPPMQLKESVFRCIMQLFD 927

Query: 921  CLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLH 980
            CLLTEVHERCKKGLSLAKRLNSSLAFFCYDLL IIEP QV++LVSLY+DKFSGVCQSVLH
Sbjct: 928  CLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEPCQVYELVSLYMDKFSGVCQSVLH 987

Query: 981  DCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARVLVVL 1040
            +CKLTFLQII DHDLFVEMPGRDPSDRNYLSS+LIQELFL+ DHD+LPLRAK AR+LV+L
Sbjct: 988  ECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQELFLSLDHDELPLRAKGARILVIL 1047

Query: 1041 LCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAVEKREVLIVILQIVRNLD 1100
            LCKHEFDARYQK EDKLYIAQLYFP +GQILDEMPVFYNLNA EKREVLI +LQIVRNLD
Sbjct: 1048 LCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVFYNLNATEKREVLIGVLQIVRNLD 1107

Query: 1101 DSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYS 1160
            D+SLVKAWQQSIARTRL+FKLMEECLILFEH+K AD +L G++SR P  V +G GSPKYS
Sbjct: 1108 DTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADSILGGNNSRGP--VSEGAGSPKYS 1167

Query: 1161 DRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQRVNSQLSSPNQPYSLRE 1220
            +RLSPAINNYLSEASRQE R+           +GTPDNGYLWQRVNSQL+SP+QPYSLRE
Sbjct: 1168 ERLSPAINNYLSEASRQEVRL-----------EGTPDNGYLWQRVNSQLASPSQPYSLRE 1227

Query: 1221 ALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQVLEITEKFSLMASSHSI 1280
            ALAQAQSSRIGASAQALRESLHP+LRQKLELWEEN+SA VSLQVLEITE FS MA+SH+I
Sbjct: 1228 ALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEITENFSSMAASHNI 1287

Query: 1281 ATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMARENDRFLKQVTFH 1340
            ATDYGKLDCIT+I  SFFS+NQ LAF+KA FP+FN +FDLHGATLMARENDRFLKQ+ FH
Sbjct: 1288 ATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMARENDRFLKQIAFH 1347

Query: 1341 LLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLIITLSELMSDVQVTQMKAN 1400
            LLRLAV+RNDS+RKRAV GLQILV+SS  +FMQTARLR +L ITLSELMSDVQVT MK++
Sbjct: 1348 LLRLAVYRNDSVRKRAVIGLQILVKSSL-YFMQTARLRALLTITLSELMSDVQVTHMKSD 1407

Query: 1401 GSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASADNRWSWSELKYLS 1460
             +LEESGEA+RL++SL +MADE+KS +LL ECGLP++ L+IIPE   +NRWSW+E+K+LS
Sbjct: 1408 NTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTENRWSWAEVKHLS 1467

Query: 1461 DSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSMDRYAAAEGFYKLAMAFA 1520
            DSL+LALDASL HALL                      SVM+MDRYAAAE FYKL MAFA
Sbjct: 1468 DSLVLALDASLGHALL---------------------GSVMAMDRYAAAESFYKLGMAFA 1527

Query: 1521 PVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRI 1580
            PVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVA V+MQALVARNDGVWS+DHV+ALR+I
Sbjct: 1528 PVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKI 1587

Query: 1581 CPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPV 1640
            CPMVS E T+EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPV
Sbjct: 1588 CPMVSGEFTTEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPV 1647

Query: 1641 YKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYR 1700
            YKSR+AYGQLAKCHTLLTNIYESIL+QES+PIPF DATYYRVGFYGEKFGKLDRKEYVYR
Sbjct: 1648 YKSRKAYGQLAKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYR 1707

Query: 1701 EPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQITAVDPVIED 1760
            EPRDVRLGDIMEKLSH+YESRMD +H LHIIPDSRQVKAE+LQ GVCYLQITAVD V+ED
Sbjct: 1708 EPRDVRLGDIMEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMED 1767

Query: 1761 EDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRSVLQTEGSFPA 1820
            EDLGSRRERI SLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRR+VLQTEGSFPA
Sbjct: 1768 EDLGSRRERIFSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPA 1827

Query: 1821 LVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSLQRILQGS 1880
            LVNRLLVTKSESLEFSPVENAIGMIETRT ALRNELEEPRSS+GD LPRLQSLQRILQGS
Sbjct: 1828 LVNRLLVTKSESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGS 1830

Query: 1881 VAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQ 1936
            VAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQ
Sbjct: 1888 VAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQ 1830

BLAST of Spg004009 vs. ExPASy Swiss-Prot
Match: Q8BIK4 (Dedicator of cytokinesis protein 9 OS=Mus musculus OX=10090 GN=Dock9 PE=1 SV=2)

HSP 1 Score: 304.7 bits (779), Expect = 7.6e-81
Identity = 374/1585 (23.60%), Postives = 646/1585 (40.76%), Query Frame = 0

Query: 435  IMMRNEPFLQLFHCLYVYPLTV------SLSRKRNLFIRIELREDDSDPRRQPLEAMYPV 494
            I    +P+    + LYVYP  +      S ++ RN+ I IE ++ D +   QPL+ +Y  
Sbjct: 638  IPKHTQPYTVYSNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEED-SQPLKCIYGR 697

Query: 495  ELGASLQKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPV 554
              G    + A   V    +   ++DEIK+ LPA    +HHLLFTFF+V      +     
Sbjct: 698  PGGPVFTRSALAAVLHHQQNPEFYDEIKIELPAQLHERHHLLFTFFHVSCDNSTKG---- 757

Query: 555  PIGYASLPLSTHAQRTRVRASSDGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASL 614
                           T+ + + + + GF+W+ L +  G  L S   +  P+ A +    L
Sbjct: 758  --------------STKKKDAVETQVGFSWLPLLK-DGRVLTSEQHI--PVSANLPSGYL 817

Query: 615  GRRLFIASRLRAIKGLVASRLRSEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRL 674
            G +     R                          HY        + ++E GK + K+  
Sbjct: 818  GYQELGMGR--------------------------HY-----GPEVKWVEGGKPLLKIST 877

Query: 675  RLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNM 734
             L S++Y  ++ + +FF +Y + T   +   GSEL++ + SL  ++   ++ FL  ILN 
Sbjct: 878  HLVSTVYTQDQHLHNFF-QYCQKTESGAQASGSELVKYLKSLHAMEGHVMIAFLPTILNQ 937

Query: 735  LLHLIGNG-GETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPV 794
            L  ++     E + V   R ++++V +  +E  E       L +YV +A+      +P V
Sbjct: 938  LFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLES-----HLRSYVKFAYK----AEPYV 997

Query: 795  YPGLSTVWGSLARSKAKGYRVGPVY---DDVLAMAWFFLELIVKSMALEKTRLFYHSLPL 854
                 TV   L +S     +    +   + +L  +WFF ++++KSMA        H +  
Sbjct: 998  ASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMA-------QHLIEN 1057

Query: 855  GGFKVLCSFMFCVTSVSFDLYCEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLA 914
               K+L +  F                          +  + + L+  + ++ +     +
Sbjct: 1058 NKVKLLRNQRF-------------------PASYHHAVETVVNMLMPHITQKFRDNPEAS 1117

Query: 915  KRLNSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFV 974
            K  N SLA F     + ++   VF  ++ Y+  F+      L + K  FL+++C+H+ ++
Sbjct: 1118 KNANHSLAVFIKRCFTFMDRGFVFKQINNYISCFAPGDPKTLFEYKFEFLRVVCNHEHYI 1177

Query: 975  EM-------PGR--------------DPSDRN-YLSSVLIQELFLTWDHDDLPLRAKAAR 1034
             +        GR              D   RN +L  +L++E+  T   +   +R  A  
Sbjct: 1178 PLNLPMPFGKGRIQRYQDLQLDYSLTDEFCRNHFLVGLLLREVG-TALQEFREVRVIAIS 1237

Query: 1035 VLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQIL---------DEMPVFYNLNAVEKR 1094
            +L  LL KH FD RY     +  IA LY PL G ++         D  P   N  ++ K 
Sbjct: 1238 MLKNLLIKHSFDDRYNSRSHQARIATLYLPLFGLLIENVQRINVRDVSPFPVNPGSIVKD 1297

Query: 1095 EVLIV------ILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLM 1154
            E L V      +     N  D SL K    +I+     +      +    +      ++ 
Sbjct: 1298 EALAVPAGNPLMTPQKGNTLDHSLHKDLLGAISGIASPYTASTPNINSVRNADSRGSLIS 1357

Query: 1155 GSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASR-QEFRVGTFLRDPLCVAQGTPDNG 1214
              S  S         +     + S  + N +    +  +  + + L   L V +   D+ 
Sbjct: 1358 TDSGNSLPDRNPEKSNSLDKQQQSGMLGNSVVRCDKLDQSEIKSLLMCFLYVLKSMSDDA 1417

Query: 1215 YL--WQRVNS---------------QLSSPNQPYSLREALAQAQSSRIGASAQALR---- 1274
                W + ++               Q     + Y  R  +  A+  ++G+   ++     
Sbjct: 1418 LFTYWNKASTAELMDFFTISEVCLHQFQYMGKRYIARTGMMHARLQQLGSLDNSVTFNHS 1477

Query: 1275 --ESLHPVLRQKLELWEENLSAAVSLQVLEITEKFSLMASSHSIATDYGK---LDCITSI 1334
               S   V+ Q   L E N++  V L  L+    F+L A  + +  D+G    +  +  +
Sbjct: 1478 YGHSEADVVHQ--SLLEANIATEVCLTALDTLSLFTL-AFKNQLLADHGHNPLMKKVFDV 1537

Query: 1335 FMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIR 1394
            ++ F  K+Q     K +F    S+     +       D     + + +L+    +  SIR
Sbjct: 1538 YLCFLQKHQSEMALKNVFTALRSLIYKFPSAFYEGRAD-MCASLCYEVLKCCNSKLSSIR 1597

Query: 1395 KRAVTGLQILVRSSFCHFMQTA--RLRVMLIITLSELMSDVQVTQMKANGSLEESGEAQR 1454
              A   L  L+R++F +  + +  R  + +II++S+L++DV    +   G+        R
Sbjct: 1598 TEASQLLYFLMRNNFDYTGKKSFVRTHLQVIISVSQLIADV----VGIGGT--------R 1657

Query: 1455 LRKSLEDMADESKSSDLLNECGLPENALVIIPEASADNRWSWSELKYLSD---SLLLALD 1514
             ++SL           ++N C    N+  II   S       S++K L+    ++L+A  
Sbjct: 1658 FQQSL----------SIINNCA---NSDRIIKHTSFS-----SDVKDLTKRIRTVLMATA 1717

Query: 1515 ASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSMDRYAAAEGFYKLAMAFAPVPDLHIM 1574
               EH                              D     +  Y LA ++A  P+L   
Sbjct: 1718 QMKEH----------------------------ENDPEMLVDLQYSLAKSYASTPELRKT 1777

Query: 1575 WLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMVSSEI 1634
            WL  +   H +    +EAA C V V A+V + L  +      R   TA R I P     I
Sbjct: 1778 WLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRKG---MFRQGCTAFRVITP----NI 1837

Query: 1635 TSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYG 1694
              EAS  E  G        D  ++ L+       +AE Y   A I +L+IP+Y+ RR + 
Sbjct: 1838 DEEASMMEDVGMQDVHFNEDVLMELLEQCADGLWKAERYELIADIYKLIIPIYEKRRDFE 1897

Query: 1695 QLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEK---------------FGKLD 1754
            +LA  +  L   Y  + E   S       TY+RV F+G+                F   D
Sbjct: 1898 RLAHLYDTLHRAYSKVTEVMHSGRRLL-GTYFRVAFFGQAAQYQFTDSETDVEGFFEDED 1957

Query: 1755 RKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQITA 1814
             KEY+Y+EP+   L +I ++L  +Y  +  GS  + +I DS +V  ++L     Y+Q+T 
Sbjct: 1958 GKEYIYKEPKLTPLSEISQRLLKLYSDKF-GSENVKMIQDSGKVNPKDLDSKFAYIQVTH 2017

Query: 1815 VDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRSVLQ 1874
            V P  ++++L  RR       T   R     RF+F+ PFT+ GK QGG+E+Q KRR++L 
Sbjct: 2018 VTPFFDEKELQERR-------TEFERCHNIRRFMFEMPFTQTGKRQGGVEEQCKRRTILT 2047

Query: 1875 TEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSL 1926
                FP +  R+ V      + +P+E AI  +  + A LR       S+E D +     L
Sbjct: 2078 AIHCFPYVKKRIPVMYQHHTDLNPIEVAIDEMSKKVAELRQLCS---SAEVDMI----KL 2047

BLAST of Spg004009 vs. ExPASy Swiss-Prot
Match: Q9BZ29 (Dedicator of cytokinesis protein 9 OS=Homo sapiens OX=9606 GN=DOCK9 PE=1 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 4.1e-79
Identity = 370/1611 (22.97%), Postives = 649/1611 (40.29%), Query Frame = 0

Query: 435  IMMRNEPFLQLFHCLYVYPLTV------SLSRKRNLFIRIELREDDSDPRRQPLEAMYPV 494
            I    +P+    + LYVYP  +      S ++ RN+ I IE ++ D +   QPL+ +Y  
Sbjct: 629  IPKHTQPYTIYTNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEED-SQPLKCIYGR 688

Query: 495  ELGASLQKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPV 554
              G    + A   V    +   ++DEIK+ LP     KHHLL TFF+V            
Sbjct: 689  PGGPVFTRSAFAAVLHHHQNPEFYDEIKIELPTQLHEKHHLLLTFFHVSCD--------- 748

Query: 555  PIGYASLPLSTHAQRTRVRASSDGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASL 614
                     ++    T+ R   + + G++W+ L +                         
Sbjct: 749  ---------NSSKGSTKKRDVVETQVGYSWLPLLK------------------------- 808

Query: 615  GRRLFIASRLRAIKGLVASRLRSEISLPVMRELVPHYL--QDTNRER-----LDYLEDGK 674
                          G V +   SE  +PV   L   YL  Q+    R     + +++ GK
Sbjct: 809  -------------DGRVVT---SEQHIPVSANLPSGYLGYQELGMGRHYGPEIKWVDGGK 868

Query: 675  NIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQF 734
             + K+   L S++Y  ++ + +FF +Y + T   +   G+EL++ + SL  ++   ++ F
Sbjct: 869  PLLKISTHLVSTVYTQDQHLHNFF-QYCQKTESGAQALGNELVKYLKSLHAMEGHVMIAF 928

Query: 735  LHPILNMLLHLIGNG-GETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDF 794
            L  ILN L  ++     E + V   R ++++V +  +E  E       L +YV YA+   
Sbjct: 929  LPTILNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLES-----HLRSYVKYAYK-- 988

Query: 795  GGRQPPVYPGLSTVWGSLARSKAKGYRVGPVY---DDVLAMAWFFLELIVKSMALEKTRL 854
               +P V     TV   L +S     +    +   + +L  +WFF ++++KSMA      
Sbjct: 989  --AEPYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMA------ 1048

Query: 855  FYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIPPMQLKEGVFRCIMQLYDCLLTEVHERC 914
              H +     K+L +  F                          +  + + L+  + ++ 
Sbjct: 1049 -QHLIENSKVKLLRNQRF-------------------PASYHHAVETVVNMLMPHITQKF 1108

Query: 915  KKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQII 974
            +     +K  N SLA F     + ++   VF  ++ Y+  F+      L + K  FL+++
Sbjct: 1109 RDNPEASKNANHSLAVFIKRCFTFMDRGFVFKQINNYISCFAPGDPKTLFEYKFEFLRVV 1168

Query: 975  CDHDLFVEM-------PGR--------------DPSDRN-YLSSVLIQELFLTWDHDDLP 1034
            C+H+ ++ +        GR              D   RN +L  +L++E+  T   +   
Sbjct: 1169 CNHEHYIPLNLPMPFGKGRIQRYQDLQLDYSLTDEFCRNHFLVGLLLREVG-TALQEFRE 1228

Query: 1035 LRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAVEKREV 1094
            +R  A  VL  LL KH FD RY     +  IA LY PL G +++      N+  +  R+V
Sbjct: 1229 VRLIAISVLKNLLIKHSFDDRYASRSHQARIATLYLPLFGLLIE------NVQRINVRDV 1288

Query: 1095 LIVILQIVRNLDDSS--------LVKAWQQSIARTRLFFKLMEECLIL---FEHRKPADG 1154
                +     + D S        LV   + S     L   L+     +   +    P   
Sbjct: 1289 SPFPVNAGMTVKDESLALPAVNPLVTPQKGSTLDNSLHKDLLGAISGIASPYTTSTPNIN 1348

Query: 1155 MLMGSSSR-SPAAVGDGPGSPKYSDRLSPAINNYLSEAS----------RQEFRVGTFLR 1214
             +  + SR S  +   G   P+ +   S +++ +   ++            +  + + L 
Sbjct: 1349 SVRNADSRGSLISTDSGNSLPERNSEKSNSLDKHQQSSTLGNSVVRCDKLDQSEIKSLLM 1408

Query: 1215 DPLCVAQGTPDNGYL--WQRVNS---------------QLSSPNQPYSLREALAQAQSSR 1274
              L + +   D+     W + ++               Q     + Y  R  +  A+  +
Sbjct: 1409 CFLYILKSMSDDALFTYWNKASTSELMDFFTISEVCLHQFQYMGKRYIARTGMMHARLQQ 1468

Query: 1275 IGASAQALR------ESLHPVLRQKLELWEENLSAAVSLQVLEITEKFSLMASSHSIATD 1334
            +G+   +L        S   VL Q   L E N++  V L  L+    F+L A  + +  D
Sbjct: 1469 LGSLDNSLTFNHSYGHSDADVLHQ--SLLEANIATEVCLTALDTLSLFTL-AFKNQLLAD 1528

Query: 1335 YGK---LDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMARENDRFLKQVTFH 1394
            +G    +  +  +++ F  K+Q     K +F    S+     +T      D     + + 
Sbjct: 1529 HGHNPLMKKVFDVYLCFLQKHQSETALKNVFTALRSLIYKFPSTFYEGRAD-MCAALCYE 1588

Query: 1395 LLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTA--RLRVMLIITLSELMSDVQVTQMK 1454
            +L+    +  SIR  A   L  L+R++F +  + +  R  + +II++S+L++DV    + 
Sbjct: 1589 ILKCCNSKLSSIRTEASQLLYFLMRNNFDYTGKKSFVRTHLQVIISVSQLIADV----VG 1648

Query: 1455 ANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASADNRWSWSELKY 1514
              G+        R ++SL           ++N C                          
Sbjct: 1649 IGGT--------RFQQSL----------SIINNCA------------------------- 1708

Query: 1515 LSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSMDRYAAAEGFYKLAMA 1574
             SD L+     S +   L K++          V +   +      D     +  Y LA +
Sbjct: 1709 NSDRLIKHTSFSSDVKDLTKRIR--------TVLMATAQMKEHENDPEMLVDLQYSLAKS 1768

Query: 1575 FAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWS-----RDH 1634
            +A  P+L   WL  +   H +    +EAA C V V A+V + L  +    W        H
Sbjct: 1769 YASTPELRKTWLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRKEAVQWEPPLLPHSH 1828

Query: 1635 VTALRR-----------ICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFS 1694
               LRR              +++  I  EAS  E  G        D  ++ L+       
Sbjct: 1829 SACLRRSRGGVFRQGCTAFRVITPNIDEEASMMEDVGMQDVHFNEDVLMELLEQCADGLW 1888

Query: 1695 QAELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRV 1754
            +AE Y   A I +L+IP+Y+ RR + +LA  +  L   Y  + E   S       TY+RV
Sbjct: 1889 KAERYELIADIYKLIIPIYEKRRDFERLAHLYDTLHRAYSKVTEVMHSGRRLL-GTYFRV 1948

Query: 1755 GFYGEK---------------FGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHT 1814
             F+G+                F   D KEY+Y+EP+   L +I ++L  +Y  +  GS  
Sbjct: 1949 AFFGQAAQYQFTDSETDVEGFFEDEDGKEYIYKEPKLTPLSEISQRLLKLYSDKF-GSEN 2008

Query: 1815 LHIIPDSRQVKAEELQPGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFL 1874
            + +I DS +V  ++L     Y+Q+T V P  ++++L  R+       T   R+    RF+
Sbjct: 2009 VKMIQDSGKVNPKDLDSKYAYIQVTHVIPFFDEKELQERK-------TEFERSHNIRRFM 2061

Query: 1875 FDTPFTKNGKTQGGLEDQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIET 1926
            F+ PFT+ GK QGG+E+Q KRR++L     FP +  R+ V      + +P+E AI  +  
Sbjct: 2069 FEMPFTQTGKRQGGVEEQCKRRTILTAIHCFPYVKKRIPVMYQHHTDLNPIEVAIDEMSK 2061

BLAST of Spg004009 vs. ExPASy Swiss-Prot
Match: Q5JSL3 (Dedicator of cytokinesis protein 11 OS=Homo sapiens OX=9606 GN=DOCK11 PE=1 SV=2)

HSP 1 Score: 292.4 bits (747), Expect = 3.9e-77
Identity = 363/1593 (22.79%), Postives = 663/1593 (41.62%), Query Frame = 0

Query: 441  PFLQLFHCLYVYPLTV------SLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASL 500
            PF    + LYVYPL +      + ++ RN+ + +E R+ D +     L+ +Y    G+  
Sbjct: 635  PFTIYKNHLYVYPLQLKYDSQKTFAKARNIAVCVEFRDSD-ESDASALKCIYGKPAGSVF 694

Query: 501  QKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYAS 560
               A+  V+   +   ++DEIK+ LP     KHHLLFTF++V  +   +           
Sbjct: 695  TTNAYAVVSHHNQNPEFYDEIKIELPIHLHQKHHLLFTFYHVSCEINTKG---------- 754

Query: 561  LPLSTHAQRTRVRASSDGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFI 620
                     T+ + + +   GFAWV L       L+ G           R+ +       
Sbjct: 755  --------TTKKQDTVETPVGFAWVPL-------LKDG-----------RIITF------ 814

Query: 621  ASRLRAIKGLVASRLRSEISLPVMRELVPHYLQDTNRER-------LDYLEDGKNIFKLR 680
                             E  LPV   L P YL   + E        + +++  K + K++
Sbjct: 815  -----------------EQQLPVSANLPPGYLNLNDAESRRQCNVDIKWVDGAKPLLKIK 874

Query: 681  LRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILN 740
              L S++Y  +  +  FF  + +     S     EL++ +  L  ++   ++QFL  IL 
Sbjct: 875  SHLESTIYTQDLHVHKFF-HHCQLIQSGSKEVPGELIKYLKCLHAMEIQVMIQFLPVILM 934

Query: 741  MLLHLIGN--GGETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQP 800
             L  ++ N    + + +     +++IV++  +E  +      +L +++ Y+F       P
Sbjct: 935  QLFRVLTNMTHEDDVPINCTMVLLHIVSKCHEEGLDS-----YLRSFIKYSFRPEKPSAP 994

Query: 801  P---VYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIVKSMA---LEKTRLFY 860
                ++  L+T   ++ +  A    +    + +L  +WFF E+I KSMA   LE+ ++  
Sbjct: 995  QAQLIHETLATTMIAILKQSADFLSI----NKLLKYSWFFFEIIAKSMATYLLEENKI-- 1054

Query: 861  HSLPLGGFKVLCSFMFCVTSVSFDLYCEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKK 920
              LP G                           +  E     +  L   ++  V  R  +
Sbjct: 1055 -KLPRG--------------------------QRFPETYHHVLHSLLLAIIPHVTIRYAE 1114

Query: 921  GLSLAKRLNSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICD 980
                ++ +N SLA F    L++++   +F+L++ Y+  FS     VL + K  FLQ IC+
Sbjct: 1115 IPDESRNVNYSLASFLKRCLTLMDRGFIFNLINDYISGFSPKDPKVLAEYKFEFLQTICN 1174

Query: 981  HDLFVEMP----------------------GRDPSDRNYLSSVLIQELFLTWDHDDLPLR 1040
            H+ ++ +                         +    ++L  +L++E  +    D+  +R
Sbjct: 1175 HEHYIPLNLPMAFAKPKLQRVQDSNLEYSLSDEYCKHHFLVGLLLRETSIAL-QDNYEIR 1234

Query: 1041 AKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAVEKREVLI 1100
              A  V+  LL KH FD RYQ    +  IAQLY P +G +L+      N+  +  R+ L 
Sbjct: 1235 YTAISVIKNLLIKHAFDTRYQHKNQQAKIAQLYLPFVGLLLE------NIQRLAGRDTLY 1294

Query: 1101 VILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEH-----RKPADGMLMGSSSR 1160
                +  +         +     R  L     +     F++     R+ + G L+   + 
Sbjct: 1295 SCAAMPNSASRDEFPCGFTSPANRGSLSTD-KDTAYGSFQNGHGIKREDSRGSLIPEGAT 1354

Query: 1161 S-PAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYL--W 1220
              P     G  + + S R S +  N L      ++ + + L   L + +   ++  L  W
Sbjct: 1355 GFPDQGNTGENTRQSSTRSSVSQYNRL-----DQYEIRSLLMCYLYIVKMISEDTLLTYW 1414

Query: 1221 QRVNSQ----------LSSPNQPYSLREALAQAQSSRIGASAQALRES-LHPVLRQKLEL 1280
             +V+ Q          +   +  Y  +  +A+   + +       R+S   P LR +  +
Sbjct: 1415 NKVSPQELINILILLEVCLFHFRYMGKRNIARVHDAWLSKHFGIDRKSQTMPALRNRSGV 1474

Query: 1281 WEENLSAAVSLQVLEITEKFSLMASSHSIATD---YGKLDCITSIFMSFFSKNQPLAFYK 1340
             +  L    SL+       F+L  SS +   D      L+  T+  +S  +    ++F+ 
Sbjct: 1475 MQARLQHLSSLE-----SSFTLNHSSTTTEADIFHQALLEGNTATEVS-LTVLDTISFFT 1534

Query: 1341 ALF-----------PVFNSVFDLHGATLMARENDRFLKQV-------------------- 1400
              F           P+   VFD+H A L   +++  LK V                    
Sbjct: 1535 QCFKTQLLNNDGHNPLMKKVFDIHLAFLKNGQSEVSLKHVFASLRAFISKFPSAFFKGRV 1594

Query: 1401 ------TFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLR--VMLIITLSELM 1460
                   + +L+    +  S R  A   L +L+R++F +  +   LR  + +II +S+L+
Sbjct: 1595 NMCAAFCYEVLKCCTSKISSTRNEASALLYLLMRNNFEYTKRKTFLRTHLQIIIAVSQLI 1654

Query: 1461 SDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASADN 1520
            +DV +    + GS        R ++SL  + + + S   +     P              
Sbjct: 1655 ADVAL----SGGS--------RFQESLFIINNFANSDRPMKATAFPAEV----------- 1714

Query: 1521 RWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSMDRYAAA 1580
                 +L     ++L+A     EH                       K   M +D     
Sbjct: 1715 ----KDLTKRIRTVLMATAQMKEH----------------------EKDPEMLIDLQ--- 1774

Query: 1581 EGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVW 1640
               Y LA ++A  P+L   WL  +   H +   ++EAA C V VAA+V + L  +     
Sbjct: 1775 ---YSLAKSYASTPELRKTWLDSMAKIHVKNGDFSEAAMCYVHVAALVAEFLHRKK---L 1834

Query: 1641 SRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHF 1700
              +  +A ++I P +  E   +  A  ++ + + ++ ++     L+       +AE Y  
Sbjct: 1835 FPNGCSAFKKITPNIDEEGAMKEDAGMMDVHYSEEVLLE----LLEQCVDGLWKAERYEI 1894

Query: 1701 CASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEK- 1760
             + I +L++P+Y+ RR + +L + +  L   Y  ILE   +       T++RV FYG+  
Sbjct: 1895 ISEISKLIVPIYEKRREFEKLTQVYRTLHGAYTKILEVMHTKKRLL-GTFFRVAFYGQSF 1954

Query: 1761 FGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCY 1820
            F + D KEY+Y+EP+   L +I  +L  +Y  +  G+  + II DS +V A+EL P   +
Sbjct: 1955 FEEEDGKEYIYKEPKLTGLSEISLRLVKLYGEKF-GTENVKIIQDSDKVNAKELDPKYAH 2014

Query: 1821 LQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKR 1880
            +Q+T V P  +D++L  R+       T   R     RF+F+ P+T +GK QG +E+Q KR
Sbjct: 2015 IQVTYVKPYFDDKELTERK-------TEFERNHNISRFVFEAPYTLSGKKQGCIEEQCKR 2031

Query: 1881 RSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLP 1929
            R++L T  SFP +  R+ +   + +   P++ A   I+ +TA L+       S++ D + 
Sbjct: 2075 RTILTTSNSFPYVKKRIPINCEQQINLKPIDVATDEIKDKTAELQKLCS---STDVDMI- 2031

BLAST of Spg004009 vs. ExPASy Swiss-Prot
Match: A2AF47 (Dedicator of cytokinesis protein 11 OS=Mus musculus OX=10090 GN=Dock11 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 3.9e-77
Identity = 374/1595 (23.45%), Postives = 661/1595 (41.44%), Query Frame = 0

Query: 441  PFLQLFHCLYVYPLTV------SLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASL 500
            PF    + LYVYPL +      S ++ RN+ + +E R+ D +     L+ +Y    G+  
Sbjct: 635  PFTIYKNHLYVYPLQLKYDSQKSFAKARNIAVCVEFRDSD-ESDASALKCIYGKPAGSVF 694

Query: 501  QKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYAS 560
               A+  V+   +   ++DEIK+ LP     KHHLLFTF++V  +   +           
Sbjct: 695  TTNAYAVVSHHNQNPEFYDEIKIELPIHLHQKHHLLFTFYHVSCEINTKG---------- 754

Query: 561  LPLSTHAQRTRVRASSDGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFI 620
                     T+ + + +   GFAWV L       L+ G           R+ +L      
Sbjct: 755  --------TTKKQDTVETPVGFAWVPL-------LKDG-----------RVITL------ 814

Query: 621  ASRLRAIKGLVASRLRSEISLPVMRELVPHYLQDTNRER-------LDYLEDGKNIFKLR 680
                             E  LPV   L P YL   + E        + +++  K + K++
Sbjct: 815  -----------------EQQLPVSANLPPGYLNVNDAESRRQSNADIKWVDGAKPLLKIK 874

Query: 681  LRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILN 740
              L S++Y  +  +  FF  + +     S     EL++ +  L  ++   ++QFL  IL 
Sbjct: 875  THLESTIYTQDLHVHKFF-HHCQLIQSGSKEVPGELIKYLKCLHAMEIQVMIQFLPVILM 934

Query: 741  MLLHLIGN--GGETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQP 800
             L  ++ N    + + +     +++IV++  +E  E      +L +++ Y+F      +P
Sbjct: 935  QLFRVLTNMTHEDDVPINCTMVLLHIVSKCHEEGLES-----YLRSFIKYSFRP---EKP 994

Query: 801  P------VYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIVKSMA---LEKTR 860
                   ++  L+T   ++ +  A    +    + +L  +WFF E+I KSMA   LE+ +
Sbjct: 995  STLQAQLIHETLATTMIAILKQSADFLAI----NKLLKYSWFFFEIIAKSMATYLLEENK 1054

Query: 861  LFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIPPMQLKEGVFRCIMQLYDCLLTEVHER 920
            +    LP G       F      V   L    IP + ++                E+ + 
Sbjct: 1055 I---KLPRGQ-----RFPEAYHHVLHSLLLAIIPHVTIR--------------YAEIPDE 1114

Query: 921  CKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQI 980
             + G       N SLA F    L++++   VF+L++ Y+  FS     VL + K  FLQ 
Sbjct: 1115 SRNG-------NYSLASFLKRCLTLMDRGFVFNLINDYISGFSPKDPKVLAEYKFEFLQT 1174

Query: 981  ICDHDLFVEMP----------------------GRDPSDRNYLSSVLIQELFLTWDHDDL 1040
            IC+H+ ++ +                         +    ++L  +L++E  +    D+ 
Sbjct: 1175 ICNHEHYIPLNLPMAFAKPKLQRVQDSNLEYSLSDEYCKHHFLVGLLLRETSIAL-QDNY 1234

Query: 1041 PLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAVEKRE 1100
             +R  A  V+  LL KH FD RYQ    +  IAQLY P +G +L+      N+  +  R+
Sbjct: 1235 EIRYTAISVIKNLLIKHAFDTRYQHKNQQAKIAQLYLPFVGLLLE------NIQRLAGRD 1294

Query: 1101 VLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEH-----RKPADGMLMGS 1160
             L     +  +         +     R  L     +     F++     R+ + G L+  
Sbjct: 1295 TLYSCAAMPSSASRDEFPCGFVSPTNRGSLASD-KDTAYGSFQNGHGIKREDSRGSLIPE 1354

Query: 1161 SSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYL- 1220
                 A     PGS   + R S + ++        ++ +   L   L + +   ++  L 
Sbjct: 1355 G----ATGFPDPGSTSENTRQSSSRSSVSQYNRLDQYEIRNLLMCYLYIVKMISEDTLLT 1414

Query: 1221 -WQRVNSQ----------LSSPNQPYSLREALAQAQSSRIGASAQALRES-LHPVLRQKL 1280
             W +V+ Q          +   +  Y  +  +A+   + +       R+S   P LR + 
Sbjct: 1415 YWNKVSPQELINILVLLEVCLFHFRYMGKRNIARVHDAWLSKHFGIDRKSQTMPALRNRS 1474

Query: 1281 ELWEENLSAAVSLQVLEITEKFSLMASSHSIATDY-------GKLDCITSI----FMSFF 1340
             + +  L    SL+       F+L  SS +   D        G      S+     +SFF
Sbjct: 1475 GVMQARLQHLSSLE-----SSFTLNHSSATTEADIFHQALLEGNTATEVSLTVLDTISFF 1534

Query: 1341 S---KNQPLAFYKALFPVFNSVFDLHGATLMARENDRFLKQV------------------ 1400
            +   KNQ L       P+   VFD+H A L   +++  LK V                  
Sbjct: 1535 TQCFKNQ-LLNNDGHNPLMKKVFDIHLAFLKNGQSEVSLKHVFASLRSFISKFPSAFFKG 1594

Query: 1401 --------TFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLR--VMLIITLSE 1460
                     + +L+    +  S R  A   L +L+R++F +  +   LR  + +II +S+
Sbjct: 1595 RVNMCAAFCYEVLKCCTSKISSTRNEASALLYLLMRNNFEYTKRKTFLRTHLQIIIAVSQ 1654

Query: 1461 LMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASA 1520
            L++DV +    + GS        R ++SL  + + + S   +     P            
Sbjct: 1655 LIADVAL----SGGS--------RFQESLFIINNFANSDRPMKATAFPTEV--------- 1714

Query: 1521 DNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSMDRYA 1580
                   +L     ++L+A     EH                       K   M +D   
Sbjct: 1715 ------KDLTKRIRTVLMATAQMKEH----------------------EKDPEMLIDLQ- 1774

Query: 1581 AAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDG 1640
                 Y LA ++A  P+L   WL  +   H +   ++EAA C V VAA+V + L  +   
Sbjct: 1775 -----YSLAKSYASTPELRKTWLDSMAKIHIKNGDFSEAAMCYVHVAALVAEFLHRKK-- 1834

Query: 1641 VWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELY 1700
                   +A ++I P +  E   +  A  ++ + + ++ ++     L+       +AE Y
Sbjct: 1835 -LFPSGCSAFKKITPNIDEEGAMKEDAGMMDVHYSEEVLLE----LLEQCVDGLWKAERY 1894

Query: 1701 HFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGE 1760
               + I +L+IP+Y+ RR + +L + +  L   Y  ILE   +       T++RV FYG+
Sbjct: 1895 EVISEISKLIIPIYEKRREFEKLTQVYRTLHGAYTKILEVMHTKKRLL-GTFFRVAFYGQ 1954

Query: 1761 K-FGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGV 1820
              F + D KEY+Y+EP+   L +I  +L  +Y  +  G+  + II DS +V A+EL P  
Sbjct: 1955 SFFEEEDGKEYIYKEPKLTGLSEISLRLVKLYGEKF-GTENVKIIQDSDKVNAKELDPKF 2014

Query: 1821 CYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQW 1880
             ++Q+T V P  +D++L  R+       T   R    +RF+F+ P+T +GK QG +E+Q 
Sbjct: 2015 AHIQVTYVKPYFDDKELTERK-------TEFERNHNINRFVFEAPYTLSGKKQGCIEEQC 2031

Query: 1881 KRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQ 1929
            KRR++L T  SFP +  R+ +   + +   P++ A   I+ +TA L        S + D 
Sbjct: 2075 KRRTILTTSNSFPYVKKRIPINCEQQVNLKPIDVATDEIKDKTAELHKLCS---SVDVDM 2031

BLAST of Spg004009 vs. ExPASy TrEMBL
Match: A0A0A0K3K4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G238980 PE=3 SV=1)

HSP 1 Score: 3428.6 bits (8889), Expect = 0.0e+00
Identity = 1773/1965 (90.23%), Postives = 1793/1965 (91.25%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPKQ+GPSPLPAYEPAFDWENERSM FGQRIPETPV                       
Sbjct: 121  NVPKQIGPSPLPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDSHH SGSSKLNF  GKE SGNGS+ H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSHHLSGSSKLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA+
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAI 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECLILFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1836

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1836

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of Spg004009 vs. ExPASy TrEMBL
Match: A0A5D3BAB8 (Guanine nucleotide exchange factor SPIKE 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00800 PE=3 SV=1)

HSP 1 Score: 3412.9 bits (8848), Expect = 0.0e+00
Identity = 1767/1965 (89.92%), Postives = 1786/1965 (90.89%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPKQ+GPSPLPAYEPAFDWENERSM FGQRIPETP                        
Sbjct: 121  NVPKQIGPSPLPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDS H S SSK NF  GKE SGNGS  H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSQHLSCSSKSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA 
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAT 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECL+LFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLVLFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1836

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1836

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of Spg004009 vs. ExPASy TrEMBL
Match: A0A1S3BGL5 (guanine nucleotide exchange factor SPIKE 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489635 PE=3 SV=1)

HSP 1 Score: 3410.5 bits (8842), Expect = 0.0e+00
Identity = 1766/1965 (89.87%), Postives = 1786/1965 (90.89%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWP+LNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPYLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPKQ+GPSPLPAYEPAFDWENERSM FGQRIPETP                        
Sbjct: 121  NVPKQIGPSPLPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDS H S SSK NF  GKE SGNGS  H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSQHLSCSSKSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA 
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAT 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECL+LFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLVLFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1836

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1836

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1836

BLAST of Spg004009 vs. ExPASy TrEMBL
Match: A0A5A7U5I0 (Guanine nucleotide exchange factor SPIKE 1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold511G00690 PE=3 SV=1)

HSP 1 Score: 3406.3 bits (8831), Expect = 0.0e+00
Identity = 1766/1965 (89.87%), Postives = 1785/1965 (90.84%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPK +GPSPLPAYEPAFDWENERSM FGQRIPETP                        
Sbjct: 121  NVPK-IGPSPLPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDS H S SSK NF  GKE SGNGS  H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSQHLSCSSKSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA 
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAT 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECL+LFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLVLFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1835

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1835

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1835

BLAST of Spg004009 vs. ExPASy TrEMBL
Match: A0A1S3BH88 (guanine nucleotide exchange factor SPIKE 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489635 PE=3 SV=1)

HSP 1 Score: 3404.0 bits (8825), Expect = 0.0e+00
Identity = 1765/1965 (89.82%), Postives = 1785/1965 (90.84%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKWHNKFEENLEQWP+LNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPYLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETGNYAFQHGIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLS 120
            NQIYEGPDTDIET          EMRLTYARRTKPDDTTEDDVPSTSGRPE+TTYDPLLS
Sbjct: 61   NQIYEGPDTDIET----------EMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLS 120

Query: 121  NVPKQLGPSPLPAYEPAFDWENERSMIFGQRIPETPV----------------------- 180
            NVPK +GPSPLPAYEPAFDWENERSM FGQRIPETP                        
Sbjct: 121  NVPK-IGPSPLPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPF 180

Query: 181  -------SQERREKLSEDFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240
                   ++ERREKLSEDFHFRI PKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA
Sbjct: 181  YGTICLYNRERREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHA 240

Query: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSA 300
            TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPY+ESFAWAIVSLFDNSTGAASAGSA
Sbjct: 241  TEEGGVTASVYSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSA 300

Query: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360
            SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ
Sbjct: 301  SPSSPLAPSITGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQ 360

Query: 361  DPKYKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNG 420
            DPK+KVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKK PNNG
Sbjct: 361  DPKHKVHKPVKGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNG 420

Query: 421  SDSHHHSGSSKLNFSDGKELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLY 480
            SDS H S SSK NF  GKE SGNGS  H N D+NADDFHAFDFR+MMRNEPFLQLFHCLY
Sbjct: 421  SDSQHLSCSSKSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLY 480

Query: 481  VYPLTVSLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVAC 540
            VYPLTVSLSRKRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVAC
Sbjct: 481  VYPLTVSLSRKRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVAC 540

Query: 541  YHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASS 600
            YHDEIKLSLPATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQ        
Sbjct: 541  YHDEIKLSLPATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQ-------- 600

Query: 601  DGRGGFAWVRLTECPGECLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLR 660
                                                                      LR
Sbjct: 601  ----------------------------------------------------------LR 660

Query: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720
            SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR
Sbjct: 661  SEISLPVMRELVPHYLQDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDR 720

Query: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780
            HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN
Sbjct: 721  HTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVN 780

Query: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840
            IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP
Sbjct: 781  IVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGP 840

Query: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIP 900
            VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG                     EDIP
Sbjct: 841  VYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLG---------------------EDIP 900

Query: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960
            PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL
Sbjct: 901  PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDL 960

Query: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020
            VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD
Sbjct: 961  VSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWD 1020

Query: 1021 HDDLPLRAKAARVLVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAV 1080
            HDDLPLRAKAAR+LVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNA 
Sbjct: 1021 HDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAT 1080

Query: 1081 EKREVLIVILQIVRNLDDSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSS 1140
            EKREVLIVILQIVRNLDD+SLVKAWQQSIARTRLFFKLMEECL+LFEHRKPADG+LMGSS
Sbjct: 1081 EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLVLFEHRKPADGVLMGSS 1140

Query: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQ 1200
            SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR            QGTPDNGYLWQ
Sbjct: 1141 SRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR-----------PQGTPDNGYLWQ 1200

Query: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260
            RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ
Sbjct: 1201 RVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQ 1260

Query: 1261 VLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320
            VLEITEKFS MASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA
Sbjct: 1261 VLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGA 1320

Query: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380
            TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII
Sbjct: 1321 TLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLII 1380

Query: 1381 TLSELMSDVQVTQMKANGSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIP 1440
            TLSELMSDVQVTQMKANG+LEESGEAQRLRKSLEDMADESKSS LLNECGLPENALVIIP
Sbjct: 1381 TLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNECGLPENALVIIP 1440

Query: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSM 1500
            EASADNRWSWSELKYLSDSLLLALDASLEHALL                     ASVMSM
Sbjct: 1441 EASADNRWSWSELKYLSDSLLLALDASLEHALL---------------------ASVMSM 1500

Query: 1501 DRYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560
            DRYAAAEGFYKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA
Sbjct: 1501 DRYAAAEGFYKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVA 1560

Query: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620
            RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ
Sbjct: 1561 RNDGVWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQ 1620

Query: 1621 AELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680
            AELYHFCASILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG
Sbjct: 1621 AELYHFCASILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVG 1680

Query: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740
            FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ
Sbjct: 1681 FYGEKFGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQ 1740

Query: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLE 1800
            PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNG+TQGGLE
Sbjct: 1741 PGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLE 1800

Query: 1801 DQWKRRSVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1860
            DQWKRR+VLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE
Sbjct: 1801 DQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSE 1835

Query: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1920
            GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV
Sbjct: 1861 GDQLPRLQSLQRILQGSVAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAV 1835

Query: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1936
            CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL
Sbjct: 1921 CKRAIRVHFRLIGEEDQEFHTQLVNGFQSLTAELSHYIPAILSEL 1835

BLAST of Spg004009 vs. TAIR 10
Match: AT4G16340.1 (guanyl-nucleotide exchange factors;GTPase binding;GTP binding )

HSP 1 Score: 2798.8 bits (7254), Expect = 0.0e+00
Identity = 1460/1948 (74.95%), Postives = 1610/1948 (82.65%), Query Frame = 0

Query: 21   EENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQNQIYEGPDTDIETGNYAFQH 80
            +ENLEQWPHLN+LVQCY T+WVKD NKYGHYE I P SFQ QI+EGPDTD ET       
Sbjct: 28   DENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTET------- 87

Query: 81   GIIEMRLTYARRTKPDDTTEDDVPSTSGRPEATTYDPLLSNVPKQLGPSPLPAYEPAFDW 140
               E+RL  AR      T E+DV S SGRP +   DP  S   K  G  PLPAYEPAFDW
Sbjct: 88   ---EIRLASARSA----TIEEDVASISGRPFS---DPGSS---KHFGQPPLPAYEPAFDW 147

Query: 141  ENERSMIFGQRIPETPV---------------------------------SQERREKLSE 200
            ENER+MIFGQR PE+P                                  +QER+EKLSE
Sbjct: 148  ENERAMIFGQRTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSE 207

Query: 201  DFHFRIVPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASVYSRKEPV 260
            DF+F+I P EMQD K+S E RG+FYL+APSASVCL IQLEK ATEEGGVT+SVYSRKEPV
Sbjct: 208  DFYFQIQPTEMQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPV 267

Query: 261  HLNEREKQKLQVWSQIMPYKESFAWAIVSLFDNSTGAASAGSASPSSPLAPSITGSSSHE 320
            HL EREKQKLQVWS+IMPY+ESFAWA+V LFDN+    +  SASPSSPLAPS+T SSSH+
Sbjct: 268  HLTEREKQKLQVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHD 327

Query: 321  GVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKYKVHKPVKGVLRLE 380
            GV+EP  K+T DGK GYS GSSVVVEISNLNKVKE Y+E+++QDPK KVHKPVKGVLRLE
Sbjct: 328  GVYEPIAKITSDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLE 387

Query: 381  IEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKLPNNGSDSHHHSGSSKLNFSDG 440
            IEKH+  H D E++SE+GS+I+DS+D  DRL D T  K P++ S     +G SK N  D 
Sbjct: 388  IEKHRNGHGDFEDLSENGSIINDSLDPTDRLSDLTLMKCPSSSSGG-PRNGCSKWNSEDA 447

Query: 441  KELSGNGSYPHGNADSNADDFHAFDFRIMMRNEPFLQLFHCLYVYPLTVSLSRKRNLFIR 500
            K++S N +   G  D N   +HAFDF    RNEPFL LFHCLYVYP+ V+LSRKRN FIR
Sbjct: 448  KDVSRNLTSSCGTPDLNC--YHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIR 507

Query: 501  IELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLPATWTPKH 560
            +ELR+DD+D R+QPLEA+YP E G SLQKW HTQVAVGAR A YHDEIK+SLPATWTP H
Sbjct: 508  VELRKDDTDIRKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSH 567

Query: 561  HLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQRTRVRASSDGRGGFAWVRLTECPGE 620
            HLLFTFF+VD+Q KLEAP+PV +GYASLPLST+                           
Sbjct: 568  HLLFTFFHVDLQTKLEAPRPVVVGYASLPLSTY--------------------------- 627

Query: 621  CLQSGARLGAPLHAWVRLASLGRRLFIASRLRAIKGLVASRLRSEISLPVMRELVPHYLQ 680
                       +H+                            RS+ISLPVMRELVPHYLQ
Sbjct: 628  -----------IHS----------------------------RSDISLPVMRELVPHYLQ 687

Query: 681  DTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAI 740
            ++ +ERLDYLEDGKNIFKLRLRLCSSLYP NER+RDF LEYDRHTL+T PPWGSELL+AI
Sbjct: 688  ESTKERLDYLEDGKNIFKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAI 747

Query: 741  NSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERNH 800
            NSLK+VDSTALLQFL+PILNMLLHLIGNGGETLQVAAFRAMV+I+TRVQQ S +D +RN 
Sbjct: 748  NSLKHVDSTALLQFLYPILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNR 807

Query: 801  FLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIV 860
            FLV YVDY+FDDFGG QPPVYPGL+TVWGSLARSKAKGYRVGPVYDDVL+MAWFFLELIV
Sbjct: 808  FLVTYVDYSFDDFGGNQPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIV 867

Query: 861  KSMALEKTRLFYHSLPLGGFKVLCSFMFCVTSVSFDLYCEDIPPMQLKEGVFRCIMQLYD 920
            KSMALE+ RL+ H+LP G                     ED+PPMQLKE VFRCIMQL+D
Sbjct: 868  KSMALEQARLYDHNLPTG---------------------EDVPPMQLKESVFRCIMQLFD 927

Query: 921  CLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLH 980
            CLLTEVHERCKKGLSLAKRLNSSLAFFCYDLL IIEP QV++LVSLY+DKFSGVCQSVLH
Sbjct: 928  CLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEPCQVYELVSLYMDKFSGVCQSVLH 987

Query: 981  DCKLTFLQIICDHDLFVEMPGRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARVLVVL 1040
            +CKLTFLQII DHDLFVEMPGRDPSDRNYLSS+LIQELFL+ DHD+LPLRAK AR+LV+L
Sbjct: 988  ECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQELFLSLDHDELPLRAKGARILVIL 1047

Query: 1041 LCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYNLNAVEKREVLIVILQIVRNLD 1100
            LCKHEFDARYQK EDKLYIAQLYFP +GQILDEMPVFYNLNA EKREVLI +LQIVRNLD
Sbjct: 1048 LCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVFYNLNATEKREVLIGVLQIVRNLD 1107

Query: 1101 DSSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYS 1160
            D+SLVKAWQQSIARTRL+FKLMEECLILFEH+K AD +L G++SR P  V +G GSPKYS
Sbjct: 1108 DTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADSILGGNNSRGP--VSEGAGSPKYS 1167

Query: 1161 DRLSPAINNYLSEASRQEFRVGTFLRDPLCVAQGTPDNGYLWQRVNSQLSSPNQPYSLRE 1220
            +RLSPAINNYLSEASRQE R+           +GTPDNGYLWQRVNSQL+SP+QPYSLRE
Sbjct: 1168 ERLSPAINNYLSEASRQEVRL-----------EGTPDNGYLWQRVNSQLASPSQPYSLRE 1227

Query: 1221 ALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQVLEITEKFSLMASSHSI 1280
            ALAQAQSSRIGASAQALRESLHP+LRQKLELWEEN+SA VSLQVLEITE FS MA+SH+I
Sbjct: 1228 ALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEITENFSSMAASHNI 1287

Query: 1281 ATDYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMARENDRFLKQVTFH 1340
            ATDYGKLDCIT+I  SFFS+NQ LAF+KA FP+FN +FDLHGATLMARENDRFLKQ+ FH
Sbjct: 1288 ATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMARENDRFLKQIAFH 1347

Query: 1341 LLRLAVFRNDSIRKRAVTGLQILVRSSFCHFMQTARLRVMLIITLSELMSDVQVTQMKAN 1400
            LLRLAV+RNDS+RKRAV GLQILV+SS  +FMQTARLR +L ITLSELMSDVQVT MK++
Sbjct: 1348 LLRLAVYRNDSVRKRAVIGLQILVKSSL-YFMQTARLRALLTITLSELMSDVQVTHMKSD 1407

Query: 1401 GSLEESGEAQRLRKSLEDMADESKSSDLLNECGLPENALVIIPEASADNRWSWSELKYLS 1460
             +LEESGEA+RL++SL +MADE+KS +LL ECGLP++ L+IIPE   +NRWSW+E+K+LS
Sbjct: 1408 NTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTENRWSWAEVKHLS 1467

Query: 1461 DSLLLALDASLEHALLVKKLSWSAIIFLFNVALTMRKASVMSMDRYAAAEGFYKLAMAFA 1520
            DSL+LALDASL HALL                      SVM+MDRYAAAE FYKL MAFA
Sbjct: 1468 DSLVLALDASLGHALL---------------------GSVMAMDRYAAAESFYKLGMAFA 1527

Query: 1521 PVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRI 1580
            PVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVA V+MQALVARNDGVWS+DHV+ALR+I
Sbjct: 1528 PVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKI 1587

Query: 1581 CPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPV 1640
            CPMVS E T+EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPV
Sbjct: 1588 CPMVSGEFTTEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPV 1647

Query: 1641 YKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYR 1700
            YKSR+AYGQLAKCHTLLTNIYESIL+QES+PIPF DATYYRVGFYGEKFGKLDRKEYVYR
Sbjct: 1648 YKSRKAYGQLAKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYR 1707

Query: 1701 EPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQITAVDPVIED 1760
            EPRDVRLGDIMEKLSH+YESRMD +H LHIIPDSRQVKAE+LQ GVCYLQITAVD V+ED
Sbjct: 1708 EPRDVRLGDIMEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMED 1767

Query: 1761 EDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRSVLQTEGSFPA 1820
            EDLGSRRERI SLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRR+VLQTEGSFPA
Sbjct: 1768 EDLGSRRERIFSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPA 1827

Query: 1821 LVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSLQRILQGS 1880
            LVNRLLVTKSESLEFSPVENAIGMIETRT ALRNELEEPRSS+GD LPRLQSLQRILQGS
Sbjct: 1828 LVNRLLVTKSESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGS 1830

Query: 1881 VAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQ 1936
            VAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQ
Sbjct: 1888 VAVQVNSGVLSVCTAFLSGEPATRLRSQELQQLIAALLEFMAVCKRAIRVHFRLIGEEDQ 1830