Spg003832 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003832
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionlysine-specific demethylase JMJ25
Locationscaffold4: 44457954 .. 44460981 (+)
RNA-Seq ExpressionSpg003832
SyntenySpg003832
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGATCAAAATGGATTCACATTGCAGGTATATTTATTGGTTCATACTCATTCAGTGAAGCCTAAAGATGCTCAGGGGATTGATATTGAGTGCACAGAAAATGCCGCTTTGAAATCTGTGGCGAATGAGTTGCATAGTGATGAAGAACCGTGTTCTAGTGACGGGAGATCAGCAGACCTATTAGTTCATGGTCATGGGTTGCAGGATGAGCACGATGCTAGGGGTGAAGCAGAGACAGAAGTCACAGTGTTAAGTGACAAGATGGAATCTAATAGCGCTGATGAGCAAGCTGCTAACTCCAAAATGTCAAATAGAGATATCTTTAAAAAGACTTCTTCGACAGTTGTTTGGGATGTCTATCGGCGGATGGATGTTCCCAAATTGACTGAGTATTTAAGACTACATTGGAAGGAATTTGAGAAGTCTGTCAACATAAACGATGATCTAGTAAGTTATTCCTCCCTCCCTCCCACACTTTTAAATAATGGGGAAATAGTTTGTAAATTATTTCACTTGCGATTTCCTTGAGTATCAGGTTTTGCGGCCTCTTTATGATGGAGAATTGTGCCTTGATGGACATCATAAAAGGAAATTGAAAGATGAATTCGGTAAACCTCGTCTGATTTTCTTAAGCTTTCATTGTGTATATTAGAGAGAAAATGCTATTTGTACAGTCTTTGCTTAAATTTCCATAGAGAATGTTTACAATAAGGAGCAAGTGGGTTTAGTTTTAAGGGGAATGGAGATAAATTATGGATGTTGGTCATTTCTTGCATAAACCACGTAGATAACTTCTCAAGAGTGTATTTTCTGAACTTGAGCTAGTCACACTCCTTTTTTTTTTTTTTTTCTTTCCGTCTTCTTCTCTCTCTGCTTTGCACAGAAATGAAATTGAACAAAAGTTTCCTCCTATGCATCTTAATTCCTCTCCTATAAATTCTTTACAAATGCTTGTCTCTTTCAGGAGTAGAGCCTTGGACATTTGAGCAGCGTTTGGGTGAAGCTGTTTTTATCCCTTCAGGTTGCCCTTTTCAAGTCATGAATCTTCAGGTTGTTCGTCTGTTTCTTTACATTTTGATTGATGACAGTTATTCTGATCAAAACATTTATCAACTGCCTCTCCACAAATGAGGCCAAACCACCCAACAAATAAGAAGCAAATGTGCAATGATGCCTATCTTACCTGGTGAAATTTTGAATACTAAAGTTTAACTTCCCTATTGGTAAATAAAGGAACTAAATTCTTGAAGTTTTCTAAACTTGATGACTTGTAGAATAAGGGTGAGTTTGGAATGCCGTTTGAAAAAAGTGCTCTGATGTGAAACACTTATTTTGTAAGTAATGGAAGAAAACTAATTAAATTCTTAATCTAAGAGCACTTTAAGTGCTTTTATCTGTTTTTAAAATTTCTCAAACTACTTTTAATTATAGAAAATTTGGGAAAGTGATTTTAAAGAGTTAAAAGCATTATCTAAAATGTAAACCAAACACTACCAATTTAAAAATAAACACCTTTAATCCTCCTAAGCACTTTAAAAGACATGCCTAACACATATTATTTCAGTAACCACATCTTGATTCTTGAGCTCATAGTTCGACTTAATTATTTTTCCTTGAGCAAACACATTTTTTTGTTGGACATGGGAATGGATTTAAACTTATTTATTTGTTTTCCTAAGAAGTTTGATTCTTTTGGTTCTTTTGGAACGTACGGTACCATTTCTATTAATTGAATCAATATTCATATATAGTGTGTTCTCTTCCAATCAATATTCATATATACTGGACAGTGAATATCGAAGTCCAGAAGTTTGTGATGGAAGACATTTTCCCTAAAATTTGATTTGCTAACCATGTTACAATAGGCTTCTTGCTTTCCAAATTGCCTCCCAGGCTATCTCAACAGTCGACAGGCAATTTGGAATTGCTAGGCGATATTTACTTCTGATAACTCATGGTAATTTGATTGCTCGTCACAGAAGTAGAGTTATCAAAATGTGCTATTTTACTCTTTAGATGTGTAAAGTAGAATTTGATTCTAGGTTGTTTTATCATCATATTTCTGGGCAATAGGCAGCAGGAGTTATGTTGTTGGTCTTCTTATACTTTGTTTACTCTTTGCAGTCCAATGTTCAATTGGGTCTTGATTTCTTATCTCCTGAAAGTGTTGGTGAGGCTGCAAGAATGGCTGCTGATATCAGGTGTCTTCCTAATGATCATGAAGCAAAACTGCAAGTACTGGAGGTCAGAGACAAGGACCGTTTTGAATTTAATTTGTTTAGTTTTAATCTCATTTGGTCTATATATATTTTTTAATATCCGTGAGTGTTCAGAACAACTTACATGCATTGATGTATCTTGATTAATCTCACGAACCGGTTGCTTGGTCTGGTGTAGGTTGGAAAAATCTCACTTTATGCAGCAAGCTCAGTTATCAAAGAAGTACAGAAATTGGTGCTAGATCCAAAGTGAGATGCTCTGTCCTTCTAACTTTTGTTGTTTTTGTTGTCAAATCCTTGATCAGTATGAAAGAAGAAACTGTTAAGAGAGATCCTAATGATGTTTGATTCCTCGTTAGGTTCTATGTTTCTCTTTAGCCGTGATGAAGATTTGTTGTAATTATACTTTAGATCTTATTTTACTTGATTGGAACCTCTTTCTTTAGTTGGATTGGATTTGCTTCTGTGAGCTTGATTTTAAGGGTTTGGTTTTTTGGATACCCTTGTATTCTTTTATTTCTTTTTCTATGAGAGTTTGCGTCTCTATACCAAAAAAAAAAAAAAAAAAGAACTGTTTAAATAGAAAACGATTTTGGATTAAACTTAAATGCTCATTTTTCTCATGAACATGAAATAAACTCATTATATATTTGCGTTATTGTTCTTCACATCAACTTGAAGCACTTATGTTTTCCAATTCTCCCAGATTAAGTGAAGAGCTTGGAATCGGGGACCCTAATTTGACTGCTGCTGTTTCTGAAAACTTAGAGGAGGTGACAAAGCGAAGGCAGATAAGTTGTGCTTAG

mRNA sequence

ATGATGGATCAAAATGGATTCACATTGCAGGTATATTTATTGGTTCATACTCATTCAGTGAAGCCTAAAGATGCTCAGGGGATTGATATTGAGTGCACAGAAAATGCCGCTTTGAAATCTGTGGCGAATGAGTTGCATAGTGATGAAGAACCGTGTTCTAGTGACGGGAGATCAGCAGACCTATTAGTTCATGGTCATGGGTTGCAGGATGAGCACGATGCTAGGGGTGAAGCAGAGACAGAAGTCACAGTGTTAAGTGACAAGATGGAATCTAATAGCGCTGATGAGCAAGCTGCTAACTCCAAAATGTCAAATAGAGATATCTTTAAAAAGACTTCTTCGACAGTTGTTTGGGATGTCTATCGGCGGATGGATGTTCCCAAATTGACTGAGTATTTAAGACTACATTGGAAGGAATTTGAGAAGTCTGTCAACATAAACGATGATCTAGTTTTGCGGCCTCTTTATGATGGAGAATTGTGCCTTGATGGACATCATAAAAGGAAATTGAAAGATGAATTCGGAGTAGAGCCTTGGACATTTGAGCAGCGTTTGGGTGAAGCTGTTTTTATCCCTTCAGGTTGCCCTTTTCAAGTCATGAATCTTCAGTCCAATGTTCAATTGGGTCTTGATTTCTTATCTCCTGAAAGTGTTGGTGAGGCTGCAAGAATGGCTGCTGATATCAGGTGTCTTCCTAATGATCATGAAGCAAAACTGCAAGTACTGGAGGTTGGAAAAATCTCACTTTATGCAGCAAGCTCAGTTATCAAAGAAGTACAGAAATTGGTGCTAGATCCAAAATTAAGTGAAGAGCTTGGAATCGGGGACCCTAATTTGACTGCTGCTGTTTCTGAAAACTTAGAGGAGGTGACAAAGCGAAGGCAGATAAGTTGTGCTTAG

Coding sequence (CDS)

ATGATGGATCAAAATGGATTCACATTGCAGGTATATTTATTGGTTCATACTCATTCAGTGAAGCCTAAAGATGCTCAGGGGATTGATATTGAGTGCACAGAAAATGCCGCTTTGAAATCTGTGGCGAATGAGTTGCATAGTGATGAAGAACCGTGTTCTAGTGACGGGAGATCAGCAGACCTATTAGTTCATGGTCATGGGTTGCAGGATGAGCACGATGCTAGGGGTGAAGCAGAGACAGAAGTCACAGTGTTAAGTGACAAGATGGAATCTAATAGCGCTGATGAGCAAGCTGCTAACTCCAAAATGTCAAATAGAGATATCTTTAAAAAGACTTCTTCGACAGTTGTTTGGGATGTCTATCGGCGGATGGATGTTCCCAAATTGACTGAGTATTTAAGACTACATTGGAAGGAATTTGAGAAGTCTGTCAACATAAACGATGATCTAGTTTTGCGGCCTCTTTATGATGGAGAATTGTGCCTTGATGGACATCATAAAAGGAAATTGAAAGATGAATTCGGAGTAGAGCCTTGGACATTTGAGCAGCGTTTGGGTGAAGCTGTTTTTATCCCTTCAGGTTGCCCTTTTCAAGTCATGAATCTTCAGTCCAATGTTCAATTGGGTCTTGATTTCTTATCTCCTGAAAGTGTTGGTGAGGCTGCAAGAATGGCTGCTGATATCAGGTGTCTTCCTAATGATCATGAAGCAAAACTGCAAGTACTGGAGGTTGGAAAAATCTCACTTTATGCAGCAAGCTCAGTTATCAAAGAAGTACAGAAATTGGTGCTAGATCCAAAATTAAGTGAAGAGCTTGGAATCGGGGACCCTAATTTGACTGCTGCTGTTTCTGAAAACTTAGAGGAGGTGACAAAGCGAAGGCAGATAAGTTGTGCTTAG

Protein sequence

MMDQNGFTLQVYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQDEHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLTEYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVFIPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLYAASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA
Homology
BLAST of Spg003832 vs. NCBI nr
Match: XP_023537534.1 (lysine-specific demethylase JMJ25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511.1 bits (1315), Expect = 6.3e-141
Identity = 255/289 (88.24%), Postives = 267/289 (92.39%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVHTHSVKPK+AQGIDIECTEN  +KSVANELHSDEE CS DGRSADLLVHGH  QD
Sbjct: 656 VYLLVHTHSVKPKEAQGIDIECTENTTVKSVANELHSDEELCSGDGRSADLLVHGHASQD 715

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A GEAETE TVLS K ESN ADEQ  NSKMS+RDIFK + S VVWDVYRR DVPKLT
Sbjct: 716 EQEAMGEAETEGTVLSQKKESNCADEQTDNSKMSDRDIFKNSYSAVVWDVYRRKDVPKLT 775

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHW+EF KSVNIN+DL++RPLYDG L LDGHHKRKLKDEFGVEPWTFEQRLGEAVF
Sbjct: 776 EYLRLHWEEFGKSVNINNDLIMRPLYDGALYLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 835

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMA DIRCLPNDHEAKLQVLEVGKISLY
Sbjct: 836 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAVDIRCLPNDHEAKLQVLEVGKISLY 895

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLS+ELG+GDPNLTAAVSENLEE+TKRRQISCA
Sbjct: 896 AASSVIKEVQKLVLDPKLSDELGVGDPNLTAAVSENLEEMTKRRQISCA 944

BLAST of Spg003832 vs. NCBI nr
Match: KAG7021099.1 (Lysine-specific demethylase JMJ25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 508.4 bits (1308), Expect = 4.1e-140
Identity = 254/289 (87.89%), Postives = 266/289 (92.04%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVHTHSVKPK+AQGIDIECTEN  +KSVANELHSDEE CS DGRSADLLVHGH  QD
Sbjct: 654 VYLLVHTHSVKPKEAQGIDIECTENTTVKSVANELHSDEELCSGDGRSADLLVHGHASQD 713

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A GEAETE TVLS K ESN ADEQ  NSKMS+RDIFK + S VVWDVYRR DVPKLT
Sbjct: 714 EQEAMGEAETEGTVLSQKKESNCADEQTDNSKMSDRDIFKNSYSAVVWDVYRRKDVPKLT 773

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHW+EF KSVNIN+DL++RPLYDG L LDGHHK KLKDEFGVEPWTFEQRLGEAVF
Sbjct: 774 EYLRLHWEEFGKSVNINNDLIMRPLYDGALYLDGHHKGKLKDEFGVEPWTFEQRLGEAVF 833

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMA DIRCLPNDHEAKLQVLEVGKISLY
Sbjct: 834 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAVDIRCLPNDHEAKLQVLEVGKISLY 893

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLS+ELG+GDPNLTAAVSENLEE+TKRRQISCA
Sbjct: 894 AASSVIKEVQKLVLDPKLSDELGVGDPNLTAAVSENLEEMTKRRQISCA 942

BLAST of Spg003832 vs. NCBI nr
Match: XP_022937904.1 (lysine-specific demethylase JMJ25 [Cucurbita moschata])

HSP 1 Score: 505.8 bits (1301), Expect = 2.6e-139
Identity = 253/289 (87.54%), Postives = 265/289 (91.70%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVHTHSVKPK+AQGIDIECTEN  +KSVANELHSDEE CS DGRSADLLVHGH  QD
Sbjct: 654 VYLLVHTHSVKPKEAQGIDIECTENTTVKSVANELHSDEELCSGDGRSADLLVHGHASQD 713

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A  EAETE TVLS K ESN ADEQ  NSKMS+RDIFK + S VVWDVYRR DVPKLT
Sbjct: 714 EQEAMDEAETEGTVLSQKKESNCADEQTDNSKMSDRDIFKNSYSAVVWDVYRRKDVPKLT 773

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHW+EF KSVNIN+DL++RPLYDG L LDGHHK KLKDEFGVEPWTFEQRLGEAVF
Sbjct: 774 EYLRLHWEEFGKSVNINNDLIMRPLYDGALYLDGHHKGKLKDEFGVEPWTFEQRLGEAVF 833

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMA DIRCLPNDHEAKLQVLEVGKISLY
Sbjct: 834 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAVDIRCLPNDHEAKLQVLEVGKISLY 893

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLS+ELG+GDPNLTAAVSENLEE+TKRRQISCA
Sbjct: 894 AASSVIKEVQKLVLDPKLSDELGVGDPNLTAAVSENLEEMTKRRQISCA 942

BLAST of Spg003832 vs. NCBI nr
Match: KAG6586256.1 (Lysine-specific demethylase JMJ25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 504.6 bits (1298), Expect = 5.9e-139
Identity = 252/289 (87.20%), Postives = 265/289 (91.70%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVHTHSVKPK+AQGIDIECTEN  +KSVANELHSDEE CS DGRSADLLVHGH  QD
Sbjct: 654 VYLLVHTHSVKPKEAQGIDIECTENTTVKSVANELHSDEELCSGDGRSADLLVHGHASQD 713

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A  EAETE TVLS K ESN ADEQ  NSKMS+RDIFK + S VVWDVYRR DVPKLT
Sbjct: 714 EQEAMDEAETEGTVLSQKKESNCADEQTDNSKMSDRDIFKNSYSAVVWDVYRRKDVPKLT 773

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHW+EF KSVNIN+DL++RPLYDG L LDGHHK KLKDEFG+EPWTFEQRLGEAVF
Sbjct: 774 EYLRLHWEEFGKSVNINNDLIMRPLYDGALYLDGHHKGKLKDEFGLEPWTFEQRLGEAVF 833

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMA DIRCLPNDHEAKLQVLEVGKISLY
Sbjct: 834 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAVDIRCLPNDHEAKLQVLEVGKISLY 893

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLS+ELG+GDPNLTAAVSENLEE+TKRRQISCA
Sbjct: 894 AASSVIKEVQKLVLDPKLSDELGVGDPNLTAAVSENLEEMTKRRQISCA 942

BLAST of Spg003832 vs. NCBI nr
Match: XP_022965568.1 (lysine-specific demethylase JMJ25 [Cucurbita maxima])

HSP 1 Score: 500.7 bits (1288), Expect = 8.5e-138
Identity = 250/289 (86.51%), Postives = 264/289 (91.35%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVHTHSVKPK+AQGIDIECTEN  +K VANELHSDEE CS DGRSADLLVHGH  QD
Sbjct: 653 VYLLVHTHSVKPKEAQGIDIECTENTTVKYVANELHSDEELCSGDGRSADLLVHGHASQD 712

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A GEAETE TVLS K ESN ADEQ  NSKMS+RDIFK + S VVWDVYR+ DVPKLT
Sbjct: 713 EQEAMGEAETEGTVLSQKKESNCADEQTDNSKMSDRDIFKNSYSAVVWDVYRQKDVPKLT 772

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHW+EF KSVNIN+DL++RPLYDG L LDGHHK KLKDEFGVEPWTFEQRLGEAVF
Sbjct: 773 EYLRLHWEEFGKSVNINNDLIMRPLYDGALYLDGHHKGKLKDEFGVEPWTFEQRLGEAVF 832

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMA DIRCLPNDHEAKLQVLEVGKISLY
Sbjct: 833 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAVDIRCLPNDHEAKLQVLEVGKISLY 892

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLS+ELG+GDPNLTA+VSENLEE+TK RQISCA
Sbjct: 893 AASSVIKEVQKLVLDPKLSDELGVGDPNLTASVSENLEEMTKGRQISCA 941

BLAST of Spg003832 vs. ExPASy Swiss-Prot
Match: Q9SSE9 (Lysine-specific demethylase JMJ25 OS=Arabidopsis thaliana OX=3702 GN=JMJ25 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 9.4e-31
Identity = 65/160 (40.62%), Postives = 98/160 (61.25%), Query Frame = 0

Query: 100 NSKMSNRDIFKKTSSTVVWDVYRRMDVPKLTEYLRLHWKEFEKSVNINDDLVLRPLYDGE 159
           NS+   +++  +T    +WD++RR D+PKL  Y+  H KEF          V+ P++D  
Sbjct: 733 NSRQQVQNV--ETDDGALWDIFRREDIPKLESYIEKHHKEFRHLYCCPVSQVVHPIHDQN 792

Query: 160 LCLDGHHKRKLKDEFGVEPWTFEQRLGEAVFIPSGCPFQVMNLQSNVQLGLDFLSPESVG 219
             L  +H  KLK+E+G+EPWTF Q+LG+AV IP GCP QV NL+S  ++ LDF+SPE+V 
Sbjct: 793 FYLTRYHIMKLKEEYGIEPWTFNQKLGDAVLIPVGCPHQVRNLKSCNKVALDFVSPENVS 852

Query: 220 EAARMAADIRCLPNDHEAKLQVLEVGKISLYAASSVIKEV 260
           E  R+    R LP +H AK   L V K+ ++A    ++++
Sbjct: 853 ECLRLTKQYRLLPPNHFAKEDKLGVKKMIVHAVDKALRDL 890

BLAST of Spg003832 vs. ExPASy Swiss-Prot
Match: Q7LBC6 (Lysine-specific demethylase 3B OS=Homo sapiens OX=9606 GN=KDM3B PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 8.5e-16
Identity = 55/161 (34.16%), Postives = 82/161 (50.93%), Query Frame = 0

Query: 87   DKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLTEYLRLHWKEFEKSVNI 146
            D+    + DE  A+     R    K     +W +Y   D  K+ E LR   +E  +    
Sbjct: 1583 DEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPP 1642

Query: 147  NDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVFIPSGCPFQVMNLQSNV 206
            + D    P++D    LD   +++L +E+GV+ W   Q LG+AVFIP+G P QV NL S +
Sbjct: 1643 DHD----PIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCI 1702

Query: 207  QLGLDFLSPESVGEAARMAADIRCLPN---DHEAKLQVLEV 245
            ++  DF+SPE V    R+  + R L N   +HE KLQV  +
Sbjct: 1703 KVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNI 1739

BLAST of Spg003832 vs. ExPASy Swiss-Prot
Match: Q6ZPY7 (Lysine-specific demethylase 3B OS=Mus musculus OX=10090 GN=Kdm3b PE=1 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 1.5e-15
Identity = 55/161 (34.16%), Postives = 82/161 (50.93%), Query Frame = 0

Query: 87   DKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLTEYLRLHWKEFEKSVNI 146
            D+    + DE  A+     R    K     +W +Y   D  K+ E LR   +E  +    
Sbjct: 1384 DEEVLKTIDEGDADEVTKQRIHDGKEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPP 1443

Query: 147  NDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVFIPSGCPFQVMNLQSNV 206
            + D    P++D    LD   +++L +E+GV+ W   Q LG+AVFIP+G P QV NL S +
Sbjct: 1444 DHD----PIHDQSWYLDQILRKRLFEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCI 1503

Query: 207  QLGLDFLSPESVGEAARMAADIRCLPN---DHEAKLQVLEV 245
            ++  DF+SPE V    R+  + R L N   +HE KLQV  +
Sbjct: 1504 KVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNI 1540

BLAST of Spg003832 vs. ExPASy Swiss-Prot
Match: Q5ZIX8 (Lysine-specific demethylase 3A OS=Gallus gallus OX=9031 GN=KDM3A PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.2e-15
Identity = 54/173 (31.21%), Postives = 88/173 (50.87%), Query Frame = 0

Query: 75   RGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLTEYLR 134
            +G+A+ E  VL   ++   +DE        +R+         +W +Y   D  K+ E+L+
Sbjct: 1141 KGQADQEEEVLK-TIQDGDSDELTIKRFTESRE-----KPGALWHIYAAKDTEKIREFLK 1200

Query: 135  LHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVFIPSG 194
               +E  +   ++ D    P++D    LD   +++L  E+GV+ W   Q LG+ VFIP+G
Sbjct: 1201 KVAEEQGQENPVDHD----PIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDVVFIPAG 1260

Query: 195  CPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPN---DHEAKLQVLEV 245
             P QV NL S +++  DF+SPE V     +  + R L +   +HE KLQV  V
Sbjct: 1261 APHQVHNLYSCIKVAEDFVSPEHVKHCFWLTQEFRYLSHTHTNHEDKLQVKNV 1303

BLAST of Spg003832 vs. ExPASy Swiss-Prot
Match: Q9Y4C1 (Lysine-specific demethylase 3A OS=Homo sapiens OX=9606 GN=KDM3A PE=1 SV=4)

HSP 1 Score: 82.4 bits (202), Expect = 9.4e-15
Identity = 56/173 (32.37%), Postives = 85/173 (49.13%), Query Frame = 0

Query: 75   RGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLTEYLR 134
            +G+ E E  VL   ++   +DE         R I  K     +W +Y   D  K+ E+L+
Sbjct: 1137 KGQCEQEEEVLK-TIQDGDSDELTI-----KRFIEGKEKPGALWHIYAAKDTEKIREFLK 1196

Query: 135  LHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVFIPSG 194
               +E  +    + D    P++D    LD   +++L  E+GV+ W   Q LG+ VFIP+G
Sbjct: 1197 KVSEEQGQENPADHD----PIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDVVFIPAG 1256

Query: 195  CPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLP---NDHEAKLQVLEV 245
             P QV NL S +++  DF+SPE V     +  + R L     +HE KLQV  V
Sbjct: 1257 APHQVHNLYSCIKVAEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNV 1299

BLAST of Spg003832 vs. ExPASy TrEMBL
Match: A0A6J1FCJ0 (lysine-specific demethylase JMJ25 OS=Cucurbita moschata OX=3662 GN=LOC111444156 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-139
Identity = 253/289 (87.54%), Postives = 265/289 (91.70%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVHTHSVKPK+AQGIDIECTEN  +KSVANELHSDEE CS DGRSADLLVHGH  QD
Sbjct: 654 VYLLVHTHSVKPKEAQGIDIECTENTTVKSVANELHSDEELCSGDGRSADLLVHGHASQD 713

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A  EAETE TVLS K ESN ADEQ  NSKMS+RDIFK + S VVWDVYRR DVPKLT
Sbjct: 714 EQEAMDEAETEGTVLSQKKESNCADEQTDNSKMSDRDIFKNSYSAVVWDVYRRKDVPKLT 773

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHW+EF KSVNIN+DL++RPLYDG L LDGHHK KLKDEFGVEPWTFEQRLGEAVF
Sbjct: 774 EYLRLHWEEFGKSVNINNDLIMRPLYDGALYLDGHHKGKLKDEFGVEPWTFEQRLGEAVF 833

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMA DIRCLPNDHEAKLQVLEVGKISLY
Sbjct: 834 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAVDIRCLPNDHEAKLQVLEVGKISLY 893

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLS+ELG+GDPNLTAAVSENLEE+TKRRQISCA
Sbjct: 894 AASSVIKEVQKLVLDPKLSDELGVGDPNLTAAVSENLEEMTKRRQISCA 942

BLAST of Spg003832 vs. ExPASy TrEMBL
Match: A0A6J1HPD4 (lysine-specific demethylase JMJ25 OS=Cucurbita maxima OX=3661 GN=LOC111465429 PE=4 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.1e-138
Identity = 250/289 (86.51%), Postives = 264/289 (91.35%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVHTHSVKPK+AQGIDIECTEN  +K VANELHSDEE CS DGRSADLLVHGH  QD
Sbjct: 653 VYLLVHTHSVKPKEAQGIDIECTENTTVKYVANELHSDEELCSGDGRSADLLVHGHASQD 712

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A GEAETE TVLS K ESN ADEQ  NSKMS+RDIFK + S VVWDVYR+ DVPKLT
Sbjct: 713 EQEAMGEAETEGTVLSQKKESNCADEQTDNSKMSDRDIFKNSYSAVVWDVYRQKDVPKLT 772

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHW+EF KSVNIN+DL++RPLYDG L LDGHHK KLKDEFGVEPWTFEQRLGEAVF
Sbjct: 773 EYLRLHWEEFGKSVNINNDLIMRPLYDGALYLDGHHKGKLKDEFGVEPWTFEQRLGEAVF 832

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMA DIRCLPNDHEAKLQVLEVGKISLY
Sbjct: 833 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAVDIRCLPNDHEAKLQVLEVGKISLY 892

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLS+ELG+GDPNLTA+VSENLEE+TK RQISCA
Sbjct: 893 AASSVIKEVQKLVLDPKLSDELGVGDPNLTASVSENLEEMTKGRQISCA 941

BLAST of Spg003832 vs. ExPASy TrEMBL
Match: A0A0A0LIT5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G030050 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.7e-136
Identity = 245/289 (84.78%), Postives = 265/289 (91.70%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVH+H VKPKDAQGIDIEC ENA +KSV NELHSDEE CS DGRSAD++VHGHGLQD
Sbjct: 615 VYLLVHSHLVKPKDAQGIDIECMENANVKSVVNELHSDEELCSGDGRSADIVVHGHGLQD 674

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           EH+AR EAETEV +L  KMESNS DEQAANSKMS+ D+ +K SS V+WDV+RR DVPKLT
Sbjct: 675 EHEARNEAETEVEMLGQKMESNSVDEQAANSKMSDMDVSEK-SSAVIWDVFRRKDVPKLT 734

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHWKEF K VNINDDL+LRPLYDG L LDGHHK KLK +FGVEPWTFEQRLGEAVF
Sbjct: 735 EYLRLHWKEFRKPVNINDDLILRPLYDGALYLDGHHKGKLKHDFGVEPWTFEQRLGEAVF 794

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           +PSGCPFQV+NLQSNVQLGLDFLSPESVGEAARMAA++RCLPNDHEAKLQVLEVGKISLY
Sbjct: 795 VPSGCPFQVVNLQSNVQLGLDFLSPESVGEAARMAAEVRCLPNDHEAKLQVLEVGKISLY 854

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLSEELG+GDPNLTAAVSENLE +TK+ QISCA
Sbjct: 855 AASSVIKEVQKLVLDPKLSEELGVGDPNLTAAVSENLENMTKQSQISCA 902

BLAST of Spg003832 vs. ExPASy TrEMBL
Match: A0A5A7U3J7 (Lysine-specific demethylase JMJ25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00870 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.8e-133
Identity = 241/289 (83.39%), Postives = 263/289 (91.00%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVH+H VKPKDAQGIDIECTENA +KSV NELHSD+E CS DGRSADL+VHG GL+D
Sbjct: 649 VYLLVHSHLVKPKDAQGIDIECTENANVKSVVNELHSDKELCSGDGRSADLVVHGQGLKD 708

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A  EAETEV +L  KMESN   EQAANSKMS+ D+ +K SS V+WDV+RR DVPKLT
Sbjct: 709 EREAMSEAETEVEMLGQKMESNIVVEQAANSKMSDMDVSEK-SSAVIWDVFRRKDVPKLT 768

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHWKEF K VNINDDL++RPL+DG L LDGHHK KLKD+FGVEPWTFEQRLGEAVF
Sbjct: 769 EYLRLHWKEFRKPVNINDDLIMRPLFDGALYLDGHHKGKLKDDFGVEPWTFEQRLGEAVF 828

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           +PSGCPFQV+NLQSNVQLGLDFLSPESVGEAARMAAD+RCLPNDHEAKLQVLEVGKISLY
Sbjct: 829 VPSGCPFQVVNLQSNVQLGLDFLSPESVGEAARMAADVRCLPNDHEAKLQVLEVGKISLY 888

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLSEELG+GDPNLTAAVSENLE +TK+RQISCA
Sbjct: 889 AASSVIKEVQKLVLDPKLSEELGVGDPNLTAAVSENLENMTKQRQISCA 936

BLAST of Spg003832 vs. ExPASy TrEMBL
Match: A0A5D3CQW7 (Lysine-specific demethylase JMJ25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00620 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.8e-133
Identity = 240/289 (83.04%), Postives = 263/289 (91.00%), Query Frame = 0

Query: 11  VYLLVHTHSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADLLVHGHGLQD 70
           VYLLVH+H VKPKDAQGIDIECTENA +KSV NELHSD+E CS DGRSADL+VHG GL+D
Sbjct: 649 VYLLVHSHLVKPKDAQGIDIECTENANVKSVVNELHSDKELCSGDGRSADLVVHGQGLKD 708

Query: 71  EHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVYRRMDVPKLT 130
           E +A  EAETEV +L  KMESN   EQAANSKMS+ ++ +K SS V+WDV+RR DVPKLT
Sbjct: 709 EREAMSEAETEVEMLGQKMESNIVVEQAANSKMSDMNVSEK-SSAVIWDVFRRKDVPKLT 768

Query: 131 EYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTFEQRLGEAVF 190
           EYLRLHWKEF K VNINDDL++RPL+DG L LDGHHK KLKD+FGVEPWTFEQRLGEAVF
Sbjct: 769 EYLRLHWKEFRKPVNINDDLIMRPLFDGALYLDGHHKGKLKDDFGVEPWTFEQRLGEAVF 828

Query: 191 IPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQVLEVGKISLY 250
           +PSGCPFQV+NLQSNVQLGLDFLSPESVGEAARMAAD+RCLPNDHEAKLQVLEVGKISLY
Sbjct: 829 VPSGCPFQVVNLQSNVQLGLDFLSPESVGEAARMAADVRCLPNDHEAKLQVLEVGKISLY 888

Query: 251 AASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKRRQISCA 300
           AASSVIKEVQKLVLDPKLSEELG+GDPNLTAAVSENLE +TK+RQISCA
Sbjct: 889 AASSVIKEVQKLVLDPKLSEELGVGDPNLTAAVSENLENMTKQRQISCA 936

BLAST of Spg003832 vs. TAIR 10
Match: AT1G09060.1 (Zinc finger, RING-type;Transcription factor jumonji/aspartyl beta-hydroxylase )

HSP 1 Score: 261.5 bits (667), Expect = 8.1e-70
Identity = 151/298 (50.67%), Postives = 189/298 (63.42%), Query Frame = 0

Query: 11  VYLLVHT---------HSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADL 70
           VYLLVHT            KP   +  D + +EN +L S   +L   E            
Sbjct: 658 VYLLVHTSEETTFERVRKTKPVPEEP-DQKMSENESLLSPEQKLRDGE------------ 717

Query: 71  LVHGHGLQDEHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVY 130
            +H   L +    + E E  +TV  + +  N  + +++ +           +    WDV+
Sbjct: 718 -LHDLSLGEASMEKNEPELALTVNPENLTENGDNMESSCT--------SSCAGGAQWDVF 777

Query: 131 RRMDVPKLTEYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTF 190
           RR DVPKL+ YL+   + F+K  NI  D V RPLY+G L L+ HHKR+L+DEFGVEPWTF
Sbjct: 778 RRQDVPKLSGYLQ---RTFQKPDNIQTDFVSRPLYEG-LFLNEHHKRQLRDEFGVEPWTF 837

Query: 191 EQRLGEAVFIPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQV 250
           EQ  GEA+FIP+GCPFQ+ NLQSN+Q+ LDFL PESVGE+AR+A +IRCLPNDHEAKLQ+
Sbjct: 838 EQHRGEAIFIPAGCPFQITNLQSNIQVALDFLCPESVGESARLAEEIRCLPNDHEAKLQI 897

Query: 251 LEVGKISLYAASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKR-RQISC 299
           LE+GKISLYAASS IKEVQKLVLDPK   ELG  D NLT AVS NL+E TKR +Q SC
Sbjct: 898 LEIGKISLYAASSAIKEVQKLVLDPKFGAELGFEDSNLTKAVSHNLDEATKRPQQNSC 929

BLAST of Spg003832 vs. TAIR 10
Match: AT1G09060.2 (Zinc finger, RING-type;Transcription factor jumonji/aspartyl beta-hydroxylase )

HSP 1 Score: 261.5 bits (667), Expect = 8.1e-70
Identity = 151/298 (50.67%), Postives = 189/298 (63.42%), Query Frame = 0

Query: 11  VYLLVHT---------HSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADL 70
           VYLLVHT            KP   +  D + +EN +L S   +L   E            
Sbjct: 658 VYLLVHTSEETTFERVRKTKPVPEEP-DQKMSENESLLSPEQKLRDGE------------ 717

Query: 71  LVHGHGLQDEHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVY 130
            +H   L +    + E E  +TV  + +  N  + +++ +           +    WDV+
Sbjct: 718 -LHDLSLGEASMEKNEPELALTVNPENLTENGDNMESSCT--------SSCAGGAQWDVF 777

Query: 131 RRMDVPKLTEYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTF 190
           RR DVPKL+ YL+   + F+K  NI  D V RPLY+G L L+ HHKR+L+DEFGVEPWTF
Sbjct: 778 RRQDVPKLSGYLQ---RTFQKPDNIQTDFVSRPLYEG-LFLNEHHKRQLRDEFGVEPWTF 837

Query: 191 EQRLGEAVFIPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQV 250
           EQ  GEA+FIP+GCPFQ+ NLQSN+Q+ LDFL PESVGE+AR+A +IRCLPNDHEAKLQ+
Sbjct: 838 EQHRGEAIFIPAGCPFQITNLQSNIQVALDFLCPESVGESARLAEEIRCLPNDHEAKLQI 897

Query: 251 LEVGKISLYAASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKR-RQISC 299
           LE+GKISLYAASS IKEVQKLVLDPK   ELG  D NLT AVS NL+E TKR +Q SC
Sbjct: 898 LEIGKISLYAASSAIKEVQKLVLDPKFGAELGFEDSNLTKAVSHNLDEATKRPQQNSC 929

BLAST of Spg003832 vs. TAIR 10
Match: AT1G09060.3 (Zinc finger, RING-type;Transcription factor jumonji/aspartyl beta-hydroxylase )

HSP 1 Score: 261.5 bits (667), Expect = 8.1e-70
Identity = 151/298 (50.67%), Postives = 189/298 (63.42%), Query Frame = 0

Query: 11  VYLLVHT---------HSVKPKDAQGIDIECTENAALKSVANELHSDEEPCSSDGRSADL 70
           VYLLVHT            KP   +  D + +EN +L S   +L   E            
Sbjct: 672 VYLLVHTSEETTFERVRKTKPVPEEP-DQKMSENESLLSPEQKLRDGE------------ 731

Query: 71  LVHGHGLQDEHDARGEAETEVTVLSDKMESNSADEQAANSKMSNRDIFKKTSSTVVWDVY 130
            +H   L +    + E E  +TV  + +  N  + +++ +           +    WDV+
Sbjct: 732 -LHDLSLGEASMEKNEPELALTVNPENLTENGDNMESSCT--------SSCAGGAQWDVF 791

Query: 131 RRMDVPKLTEYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRKLKDEFGVEPWTF 190
           RR DVPKL+ YL+   + F+K  NI  D V RPLY+G L L+ HHKR+L+DEFGVEPWTF
Sbjct: 792 RRQDVPKLSGYLQ---RTFQKPDNIQTDFVSRPLYEG-LFLNEHHKRQLRDEFGVEPWTF 851

Query: 191 EQRLGEAVFIPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIRCLPNDHEAKLQV 250
           EQ  GEA+FIP+GCPFQ+ NLQSN+Q+ LDFL PESVGE+AR+A +IRCLPNDHEAKLQ+
Sbjct: 852 EQHRGEAIFIPAGCPFQITNLQSNIQVALDFLCPESVGESARLAEEIRCLPNDHEAKLQI 911

Query: 251 LEVGKISLYAASSVIKEVQKLVLDPKLSEELGIGDPNLTAAVSENLEEVTKR-RQISC 299
           LE+GKISLYAASS IKEVQKLVLDPK   ELG  D NLT AVS NL+E TKR +Q SC
Sbjct: 912 LEIGKISLYAASSAIKEVQKLVLDPKFGAELGFEDSNLTKAVSHNLDEATKRPQQNSC 943

BLAST of Spg003832 vs. TAIR 10
Match: AT4G00990.1 (Transcription factor jumonji (jmjC) domain-containing protein )

HSP 1 Score: 160.2 bits (404), Expect = 2.5e-39
Identity = 77/161 (47.83%), Postives = 107/161 (66.46%), Query Frame = 0

Query: 110 KKTSSTVVWDVYRRMDVPKLTEYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDGHHKRK 169
           K      VWD++RR DVPKL ++L+ H  EF    N   + V+ P++D  + L    K++
Sbjct: 679 KAVHGGAVWDIFRREDVPKLIQFLKRHEHEFRHFNNEPLESVIHPIHDQTMFLSDSQKKQ 738

Query: 170 LKDEFGVEPWTFEQRLGEAVFIPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARMAADIR 229
           LK+EF +EPWTFEQ LGEAVFIP+GCP QV N QS +++ LDF++PESV E  R+  + R
Sbjct: 739 LKEEFDIEPWTFEQHLGEAVFIPAGCPHQVRNRQSCIKVALDFVAPESVEECLRLTQEFR 798

Query: 230 CLPNDHEAKLQVLEVGKISLYAASSVIKEVQKLVLDPKLSE 271
            LP DH +    LE+ KI+LYAASS I+EV+ L+   + S+
Sbjct: 799 RLPKDHSSSEDKLELKKIALYAASSAIREVKGLMQSSRRSD 839

BLAST of Spg003832 vs. TAIR 10
Match: AT1G11950.1 (Transcription factor jumonji (jmjC) domain-containing protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.3e-39
Identity = 88/221 (39.82%), Postives = 129/221 (58.37%), Query Frame = 0

Query: 45  LHSDEEPCSSDGRSADLLVHGHGLQDEHDARGEAETEVTVLSDKMESNSADEQAANSKMS 104
           +H+ E   S + RSA        L+ +H  + E E +        E N  +E+     +S
Sbjct: 669 MHTAEVTLSEEQRSAIA-----DLKQKHKQQNEKELQ--------EQNGLEEEEV---VS 728

Query: 105 NRDIFKKTSSTVVWDVYRRMDVPKLTEYLRLHWKEFEKSVNINDDLVLRPLYDGELCLDG 164
           +  +    +S  +WD+++R DVPKL EYLR H  EF  +       V  P++D    L  
Sbjct: 729 DEIVVYDETSGALWDIFKREDVPKLEEYLRKHCIEFRHTYCSRVTKVYHPIHDQSYFLTV 788

Query: 165 HHKRKLKDEFGVEPWTFEQRLGEAVFIPSGCPFQVMNLQSNVQLGLDFLSPESVGEAARM 224
            HKRKLK EFG+EPWTF Q+LGEAVFIP+GCP QV NL+S  ++ +DF+SPE++ E  R+
Sbjct: 789 EHKRKLKAEFGIEPWTFVQKLGEAVFIPAGCPHQVRNLKSCTKVAVDFVSPENIDECLRL 848

Query: 225 AADIRCLPNDHEAKLQVLEVGKISLYAASSVIKEVQKLVLD 266
             + R LP +H+A+   LE+ K+ +YA    +KEV+ L+LD
Sbjct: 849 TDEFRQLPKNHKAREDKLEIKKMVIYAVEQALKEVETLLLD 873

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023537534.16.3e-14188.24lysine-specific demethylase JMJ25 [Cucurbita pepo subsp. pepo][more]
KAG7021099.14.1e-14087.89Lysine-specific demethylase JMJ25, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022937904.12.6e-13987.54lysine-specific demethylase JMJ25 [Cucurbita moschata][more]
KAG6586256.15.9e-13987.20Lysine-specific demethylase JMJ25, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022965568.18.5e-13886.51lysine-specific demethylase JMJ25 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SSE99.4e-3140.63Lysine-specific demethylase JMJ25 OS=Arabidopsis thaliana OX=3702 GN=JMJ25 PE=1 ... [more]
Q7LBC68.5e-1634.16Lysine-specific demethylase 3B OS=Homo sapiens OX=9606 GN=KDM3B PE=1 SV=2[more]
Q6ZPY71.5e-1534.16Lysine-specific demethylase 3B OS=Mus musculus OX=10090 GN=Kdm3b PE=1 SV=2[more]
Q5ZIX84.2e-1531.21Lysine-specific demethylase 3A OS=Gallus gallus OX=9031 GN=KDM3A PE=2 SV=1[more]
Q9Y4C19.4e-1532.37Lysine-specific demethylase 3A OS=Homo sapiens OX=9606 GN=KDM3A PE=1 SV=4[more]
Match NameE-valueIdentityDescription
A0A6J1FCJ01.3e-13987.54lysine-specific demethylase JMJ25 OS=Cucurbita moschata OX=3662 GN=LOC111444156 ... [more]
A0A6J1HPD44.1e-13886.51lysine-specific demethylase JMJ25 OS=Cucurbita maxima OX=3661 GN=LOC111465429 PE... [more]
A0A0A0LIT51.7e-13684.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G030050 PE=4 SV=1[more]
A0A5A7U3J71.8e-13383.39Lysine-specific demethylase JMJ25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3CQW76.8e-13383.04Lysine-specific demethylase JMJ25 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G09060.18.1e-7050.67Zinc finger, RING-type;Transcription factor jumonji/aspartyl beta-hydroxylase [more]
AT1G09060.28.1e-7050.67Zinc finger, RING-type;Transcription factor jumonji/aspartyl beta-hydroxylase [more]
AT1G09060.38.1e-7050.67Zinc finger, RING-type;Transcription factor jumonji/aspartyl beta-hydroxylase [more]
AT4G00990.12.5e-3947.83Transcription factor jumonji (jmjC) domain-containing protein [more]
AT1G11950.14.3e-3939.82Transcription factor jumonji (jmjC) domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003347JmjC domainSMARTSM00558cupin_9coord: 6..229
e-value: 1.4E-8
score: 44.6
IPR003347JmjC domainPFAMPF02373JmjCcoord: 115..208
e-value: 6.4E-9
score: 36.3
IPR003347JmjC domainPROSITEPS51184JMJCcoord: 1..229
score: 12.518909
NoneNo IPR availableGENE3D2.60.120.650Cupincoord: 10..238
e-value: 1.1E-44
score: 154.5
NoneNo IPR availablePANTHERPTHR12549:SF17JMJC DOMAIN PROTEIN JMJ24coord: 11..288
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 118..231
IPR045109Histone demethylase JHDM2-likePANTHERPTHR12549JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEINcoord: 11..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003832.1Spg003832.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033169 histone H3-K9 demethylation
biological_process GO:0032259 methylation
cellular_component GO:0005634 nucleus
molecular_function GO:0032454 histone H3-methyl-lysine-9 demethylase activity
molecular_function GO:0008168 methyltransferase activity