Spg003820 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003820
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	calmodulin-like protein 7
Location	scaffold4: 47387403 .. 47388083 (+)
RNA-Seq Expression	Spg003820
Synteny	Spg003820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTCCCCATCCTCAATCTCCTCTTACTGGCCGTTTTGTTCGTTGCCGGCTTGCTCAATTTCCTCTTCTATTTCCCCACCAAGAGATTCTCCTCCTGGCTCCAATCCCTAAAACCCACCTCCCAAATCCCCCAACATTTCAAATCCTCCCCCGCCATCGCCGCCTCTCCCCCGCCGCCGCCCGCCGACGCCAAGGCGGAGATGAAGAGGGTCTTCGGCACGTTCGACAAGAACAGCGACGGGTTCATAACGAAGAAGGAGCTGATGGAGTCGCTGAAGAACATCAGAATGTTGGTGACAGAGGGGGAGGCAGAGGAGATGTTCAGAGGGGTGGATGAGAATGGAGATGGGCTGATTGATTTTGAGGAGTTCTGTGTTTTGGGGGAGAAATTGGAGATGAGGAGAGGGGTTGGAGAGGATGGAGATGAGGAAGATGAAGAAAAGGAAGTGCAATTGAGAGAGGCTTTTGGAGTGTTTGATAAGGATGAAGATGGGCTGATCACTGTTGAGGAATTGAGTTTGGTGCTGAATTGTTTGGGATTGAAGGAAGGGAAGAAGGTTGAGAATTGCAAGGAGATGATAAGGAAAGTGGATTTGGATGGAGATGGGATGGTGAATTTTGATGAGTTCAAGAGGATGATGAGAAGTGGAGGAGGTCTTGTTTGGGCAAAATCTTGA

mRNA sequence

ATGGAACTCCCCATCCTCAATCTCCTCTTACTGGCCGTTTTGTTCGTTGCCGGCTTGCTCAATTTCCTCTTCTATTTCCCCACCAAGAGATTCTCCTCCTGGCTCCAATCCCTAAAACCCACCTCCCAAATCCCCCAACATTTCAAATCCTCCCCCGCCATCGCCGCCTCTCCCCCGCCGCCGCCCGCCGACGCCAAGGCGGAGATGAAGAGGGTCTTCGGCACGTTCGACAAGAACAGCGACGGGTTCATAACGAAGAAGGAGCTGATGGAGTCGCTGAAGAACATCAGAATGTTGGTGACAGAGGGGGAGGCAGAGGAGATGTTCAGAGGGGTGGATGAGAATGGAGATGGGCTGATTGATTTTGAGGAGTTCTGTGTTTTGGGGGAGAAATTGGAGATGAGGAGAGGGGTTGGAGAGGATGGAGATGAGGAAGATGAAGAAAAGGAAGTGCAATTGAGAGAGGCTTTTGGAGTGTTTGATAAGGATGAAGATGGGCTGATCACTGTTGAGGAATTGAGTTTGGTGCTGAATTGTTTGGGATTGAAGGAAGGGAAGAAGGTTGAGAATTGCAAGGAGATGATAAGGAAAGTGGATTTGGATGGAGATGGGATGGTGAATTTTGATGAGTTCAAGAGGATGATGAGAAGTGGAGGAGGTCTTGTTTGGGCAAAATCTTGA

Coding sequence (CDS)

ATGGAACTCCCCATCCTCAATCTCCTCTTACTGGCCGTTTTGTTCGTTGCCGGCTTGCTCAATTTCCTCTTCTATTTCCCCACCAAGAGATTCTCCTCCTGGCTCCAATCCCTAAAACCCACCTCCCAAATCCCCCAACATTTCAAATCCTCCCCCGCCATCGCCGCCTCTCCCCCGCCGCCGCCCGCCGACGCCAAGGCGGAGATGAAGAGGGTCTTCGGCACGTTCGACAAGAACAGCGACGGGTTCATAACGAAGAAGGAGCTGATGGAGTCGCTGAAGAACATCAGAATGTTGGTGACAGAGGGGGAGGCAGAGGAGATGTTCAGAGGGGTGGATGAGAATGGAGATGGGCTGATTGATTTTGAGGAGTTCTGTGTTTTGGGGGAGAAATTGGAGATGAGGAGAGGGGTTGGAGAGGATGGAGATGAGGAAGATGAAGAAAAGGAAGTGCAATTGAGAGAGGCTTTTGGAGTGTTTGATAAGGATGAAGATGGGCTGATCACTGTTGAGGAATTGAGTTTGGTGCTGAATTGTTTGGGATTGAAGGAAGGGAAGAAGGTTGAGAATTGCAAGGAGATGATAAGGAAAGTGGATTTGGATGGAGATGGGATGGTGAATTTTGATGAGTTCAAGAGGATGATGAGAAGTGGAGGAGGTCTTGTTTGGGCAAAATCTTGA

Protein sequence

MELPILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPPPPADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEFCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVWAKS

Homology

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BLAST of Spg003820 vs. NCBI nr
Match: XP_038889113.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 306.6 bits (784), Expect = 1.8e-79
Identity = 163/223 (73.09%), Postives = 186/223 (83.41%), Query Frame = 0


Query: 4   PILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPPPPA 63
           PI+N LLL VL VAG +NFL YFPTKRFS+W QS+KP+ QI  HFKS  ++   PPPPP+
Sbjct: 3   PIMNFLLLVVLVVAGFVNFLLYFPTKRFSAWFQSIKPSPQI-SHFKSL-SLQPPPPPPPS 62

Query: 64  DAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFE 123
               E+K+VFGTFDKN+DGFITKKELMESLKN+RML+TE +AEEM +GVDENGDGLIDFE
Sbjct: 63  PPSPELKKVFGTFDKNNDGFITKKELMESLKNMRMLITEKDAEEMLKGVDENGDGLIDFE 122

Query: 124 EFCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLK 183
           EFCVLGEKL M  G  +    EDEE E +LREAFGVFDKD+DG+I+VEELSLVL  LG+ 
Sbjct: 123 EFCVLGEKLVMGFGEKKKICVEDEEDEDELREAFGVFDKDKDGMISVEELSLVLCSLGMN 182

Query: 184 EGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVWAKS 227
           EGKKVENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV A+S
Sbjct: 183 EGKKVENCKEMIKKVDLDGDGMVNFDEFKRMMRSGVSLVLAQS 223

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BLAST of Spg003820 vs. NCBI nr
Match: XP_023545557.1 (calmodulin-like protein 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.7 bits (748), Expect = 2.6e-75
Identity = 156/221 (70.59%), Postives = 184/221 (83.26%), Query Frame = 0


Query: 5   ILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPP--PPP 64
           ++NLL LAVLFVAG +NFL YFPTKRF++W QS+KP+ Q   HFK +    A PP  P P
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQ-SVHFKPT----AVPPLSPAP 63

Query: 65  ADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDF 124
              K+EMK+VFGTFDKN+DGFITKKELMESLKN+ M++TE EAEEM +GVDENGDGLIDF
Sbjct: 64  EKKKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDF 123

Query: 125 EEFCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGL 184
           EEFCVLGEKL M   V +   E+++E E +LREAFGVFDKD+DG+I+VEEL LVL+CLG+
Sbjct: 124 EEFCVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGM 183

Query: 185 KEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
            EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 NEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 219

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BLAST of Spg003820 vs. NCBI nr
Match: KAG6599380.1 (Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 291.2 bits (744), Expect = 7.7e-75
Identity = 157/226 (69.47%), Postives = 186/226 (82.30%), Query Frame = 0


Query: 5   ILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPP--PPP 64
           ++NLL LAVLFVAG +NFL YFPTKRF++W QS+KP+ Q   HFK +    A PP  P P
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQ-SVHFKPT----AVPPLSPAP 63

Query: 65  ADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDF 124
              K+EMK+VFGTFDKN DGFITKKELMESLKN+ M++TE EAEEM +GVDENGDGLIDF
Sbjct: 64  EKKKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDF 123

Query: 125 EEFCVLGEKL-----EMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVL 184
           EEFCVLGEKL     E +R V ++ ++EDE+   +LREAFGVFDKD+DG+I+VEEL LVL
Sbjct: 124 EEFCVLGEKLVMGFEEKQRRVEDEDEDEDED---ELREAFGVFDKDKDGMISVEELGLVL 183

Query: 185 NCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           +CLG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 SCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 221

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BLAST of Spg003820 vs. NCBI nr
Match: XP_022946838.1 (calmodulin-like protein 7 [Cucurbita moschata])

HSP 1 Score: 290.8 bits (743), Expect = 1.0e-74
Identity = 157/226 (69.47%), Postives = 186/226 (82.30%), Query Frame = 0


Query: 5   ILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPP--PPP 64
           ++NLL LAVLFVAG +NFL YFPTKRF++W QS+KP+ Q   HFK +    A PP  P P
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQ-SVHFKPT----AVPPLSPAP 63

Query: 65  ADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDF 124
              K+EMK+VFGTFDKN DGFITKKELMESLKN+ M++TE EAEEM +GVDENGDGLIDF
Sbjct: 64  EKKKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDF 123

Query: 125 EEFCVLGEKL-----EMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVL 184
           EEFCVLGEKL     E +R V ++ ++EDE+   +LREAFGVFDKD+DG+I+VEEL LVL
Sbjct: 124 EEFCVLGEKLVMGFEEKQRRVEDEDEDEDED---ELREAFGVFDKDKDGVISVEELGLVL 183

Query: 185 NCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           +CLG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 SCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 221

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BLAST of Spg003820 vs. NCBI nr
Match: XP_022999398.1 (calmodulin-like protein 7 [Cucurbita maxima])

HSP 1 Score: 290.4 bits (742), Expect = 1.3e-74
Identity = 154/219 (70.32%), Postives = 180/219 (82.19%), Query Frame = 0


Query: 5   ILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPPPPAD 64
           ++NLL LAVLFVAG +NFL YFPTKRF++W QS+KP+ Q   HFK  P     P P P  
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQ-SVHFK--PTAVPPPSPAPEK 63

Query: 65  AKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEE 124
            K+EMK+VFGTFDKN+DGFITKKELMESLKN+ M++TE EAEEM +GVDENGDGLIDFEE
Sbjct: 64  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 123

Query: 125 FCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
           FCVLGEKL +  G  E     ++E E +LREAFGVFDKD+DG+I+VEEL LVL+CLG+ E
Sbjct: 124 FCVLGEKLVV--GFEEKQRRIEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNE 183

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           GKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 217

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BLAST of Spg003820 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.8e-34
Identity = 76/152 (50.00%), Postives = 108/152 (71.05%), Query Frame = 0


Query: 68  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEFCV 127
           E+KRVF  FDKN DG IT KEL E+L+++ + + + E  +M   +D NGDG +D +EF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 128 LGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKK 187
           L + +          DEEDEE+E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK 
Sbjct: 65  LYKTIM---------DEEDEEEE-DMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKT 124

Query: 188 VENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           +++CK+MI+KVD+DGDG VN+ EF++MM+ GG
Sbjct: 125 LDDCKKMIKKVDVDGDGRVNYKEFRQMMKGGG 146

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BLAST of Spg003820 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 5.2e-34
Identity = 77/154 (50.00%), Postives = 107/154 (69.48%), Query Frame = 0


Query: 66  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEF 125
           +AE+ R+F  FD+N DG ITK+EL +SL+N+ + + + +  +M   +D NGDG +D EEF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 126 CVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEG 185
             L + +          +E DEE++  +REAF VFD++ DG ITVEEL  VL  LGLK+G
Sbjct: 63  GGLYQTIM---------EERDEEED--MREAFNVFDQNRDGFITVEELRSVLASLGLKQG 122

Query: 186 KKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           + +E+CK MI KVD+DGDGMVNF EFK+MM+ GG
Sbjct: 123 RTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGGG 145

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BLAST of Spg003820 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.0e-33
Identity = 76/154 (49.35%), Postives = 103/154 (66.88%), Query Frame = 0


Query: 66  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEF 125
           + E+ RVF  FDKN DG I K EL +  K++ ++V E E  EM   +D NGDG +D +EF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 126 CVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEG 185
             L +++            E++E+E  +REAF VFD++ DG IT EEL  VL  +GLK+G
Sbjct: 63  GSLYQEM-----------VEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQG 122

Query: 186 KKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           + +E+CK+MI KVD+DGDGMVNF EFK+MMR GG
Sbjct: 123 RTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGGG 145

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BLAST of Spg003820 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-32
Identity = 72/151 (47.68%), Postives = 104/151 (68.87%), Query Frame = 0


Query: 68  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEFCV 127
           E+ RVF  FDK+ DG IT KEL ES KN+ +++ E E  ++ + +D NGDG +D EEF  
Sbjct: 5   ELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGE 64

Query: 128 LGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKK 187
           L + + +        ++EDE  E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK 
Sbjct: 65  LYKTIMV--------EDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKT 124

Query: 188 VENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
           +E C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 125 LEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

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BLAST of Spg003820 vs. ExPASy Swiss-Prot
Match: Q9LX27 (Calmodulin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=CML4 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.8e-32
Identity = 90/201 (44.78%), Postives = 124/201 (61.69%), Query Frame = 0


Query: 22  FLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPPPPADAKAE----MKRVFGTFD 81
           FL Y     F+S+L  L P  ++   F  S  I    PPPP +++ E    +KRVF  FD
Sbjct: 5   FLLY---NLFNSFLLCLVP-KKLRVFFPPSWYIDDKNPPPPDESETESPVDLKRVFQMFD 64

Query: 82  KNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEFCVLGEKLEMRRG 141
           KN DG ITK+EL +SL+N+ + + + +  +M + +D NGDG +D  EF    E L     
Sbjct: 65  KNGDGRITKEELNDSLENLGIFMPDKDLIQMIQKMDANGDGCVDINEF----ESLY---- 124

Query: 142 VGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKKVENCKEMIRK 201
               G   +E++E  +R+AF VFD+D DG ITVEEL+ V+  LGLK+GK +E CKEMI +
Sbjct: 125 ----GSIVEEKEEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTLECCKEMIMQ 184

Query: 202 VDLDGDGMVNFDEFKRMMRSG 219
           VD DGDG VN+ EF +MM+SG
Sbjct: 185 VDEDGDGRVNYKEFLQMMKSG 189

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BLAST of Spg003820 vs. ExPASy TrEMBL
Match: A0A6J1G4Z1 (calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 4.9e-75
Identity = 157/226 (69.47%), Postives = 186/226 (82.30%), Query Frame = 0


Query: 5   ILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPP--PPP 64
           ++NLL LAVLFVAG +NFL YFPTKRF++W QS+KP+ Q   HFK +    A PP  P P
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQ-SVHFKPT----AVPPLSPAP 63

Query: 65  ADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDF 124
              K+EMK+VFGTFDKN DGFITKKELMESLKN+ M++TE EAEEM +GVDENGDGLIDF
Sbjct: 64  EKKKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDF 123

Query: 125 EEFCVLGEKL-----EMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVL 184
           EEFCVLGEKL     E +R V ++ ++EDE+   +LREAFGVFDKD+DG+I+VEEL LVL
Sbjct: 124 EEFCVLGEKLVMGFEEKQRRVEDEDEDEDED---ELREAFGVFDKDKDGVISVEELGLVL 183

Query: 185 NCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           +CLG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 SCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 221

[Close]

BLAST of Spg003820 vs. ExPASy TrEMBL
Match: A0A6J1KJL5 (calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 6.4e-75
Identity = 154/219 (70.32%), Postives = 180/219 (82.19%), Query Frame = 0


Query: 5   ILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPPPPAD 64
           ++NLL LAVLFVAG +NFL YFPTKRF++W QS+KP+ Q   HFK  P     P P P  
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQ-SVHFK--PTAVPPPSPAPEK 63

Query: 65  AKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEE 124
            K+EMK+VFGTFDKN+DGFITKKELMESLKN+ M++TE EAEEM +GVDENGDGLIDFEE
Sbjct: 64  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 123

Query: 125 FCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
           FCVLGEKL +  G  E     ++E E +LREAFGVFDKD+DG+I+VEEL LVL+CLG+ E
Sbjct: 124 FCVLGEKLVV--GFEEKQRRIEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNE 183

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           GKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 217

[Close]

BLAST of Spg003820 vs. ExPASy TrEMBL
Match: A0A0A0LL19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 2.0e-73
Identity = 154/222 (69.37%), Postives = 180/222 (81.08%), Query Frame = 0


Query: 4   PILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPP--- 63
           PI N LLL+VLFVAG +NFL YFP+KRFS+W QS+KP+SQI  HFKS+P +   PPP   
Sbjct: 3   PIFNFLLLSVLFVAGFINFLLYFPSKRFSAWFQSIKPSSQI-THFKSTP-LQPPPPPSPS 62

Query: 64  ----PPADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENG 123
               PP  +  EMK+VFGTFDKN DGFITKKELMESLK++RM++TE +AEEM +GVDENG
Sbjct: 63  PSPSPPPPSAMEMKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKGVDENG 122

Query: 124 DGLIDFEEFCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLV 183
           DGLIDFEEFCVLG KL M  G  E+     E++E +L++AFGVFDKD DGLI+VEELSLV
Sbjct: 123 DGLIDFEEFCVLGGKLMM--GFEENKKTSVEDEEDELKDAFGVFDKDSDGLISVEELSLV 182

Query: 184 LNCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
           L  LG+ EGK VENCKEMIRKVDLDGDGMVNFDEFK+MMR+G
Sbjct: 183 LCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNG 220

[Close]

BLAST of Spg003820 vs. ExPASy TrEMBL
Match: A0A1S3C5X4 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 3.5e-73
Identity = 153/219 (69.86%), Postives = 177/219 (80.82%), Query Frame = 0


Query: 4   PILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSS----PAIAASPP 63
           PI N LLL+VLFVAG +NFL YFPTKRF++W QS+KP+SQIP HFKS+    P     PP
Sbjct: 3   PIFNFLLLSVLFVAGFVNFLLYFPTKRFTAWFQSIKPSSQIP-HFKSTPLQPPPPPPPPP 62

Query: 64  PPPADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGL 123
           PPP  +  E+K+VFGTFDKN DGFITKKELMESLK++RM++TE +AEEM + VDENGDGL
Sbjct: 63  PPPPPSAMELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDGL 122

Query: 124 IDFEEFCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNC 183
           IDFEEFCVLGEKL M  G  E+      + E  L++AFGVFDKD DGLI+VEELSLVL  
Sbjct: 123 IDFEEFCVLGEKLLM--GFEENKKTSVGDDEEGLKDAFGVFDKDSDGLISVEELSLVLCS 182

Query: 184 LGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
           LG+ EGK VENCKEMIRKVDLDGDGMVNFDEFK+MMR+G
Sbjct: 183 LGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNG 218

[Close]

BLAST of Spg003820 vs. ExPASy TrEMBL
Match: A0A6J1CMI5 (calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 5.1e-64
Identity = 138/215 (64.19%), Postives = 171/215 (79.53%), Query Frame = 0


Query: 5   ILNLLLLAVLFVAGLLNFLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPPPPAD 64
           ++N+LLLAVLFVAGL+NFL YFPTKRFS+WL+   PT        ++ A A +PP PP  
Sbjct: 69  MMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQ-SPTK------PAAVAAAVAPPQPP-- 128

Query: 65  AKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEE 124
              E++++F TFDKN+DG+IT+KEL ESL+N    + E EAEE+F GVDENGDGLIDFEE
Sbjct: 129 --TELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEE 188

Query: 125 FCVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
           FCVL EKL       ++ +EE+E+ + +LREAFGVFDKD+DG+I+VEEL LVL+CLGLKE
Sbjct: 189 FCVLAEKLR------KEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKE 248

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           GK  ENC+EMIRKVDLDGDGMVNF+EFKRMMR+GG
Sbjct: 249 GKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNGG 263

[Close]

BLAST of Spg003820 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 147.5 bits (371), Expect = 1.3e-35
Identity = 76/152 (50.00%), Postives = 108/152 (71.05%), Query Frame = 0


Query: 68  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEFCV 127
           E+KRVF  FDKN DG IT KEL E+L+++ + + + E  +M   +D NGDG +D +EF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 128 LGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKK 187
           L + +          DEEDEE+E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK 
Sbjct: 65  LYKTIM---------DEEDEEEE-DMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKT 124

Query: 188 VENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           +++CK+MI+KVD+DGDG VN+ EF++MM+ GG
Sbjct: 125 LDDCKKMIKKVDVDGDGRVNYKEFRQMMKGGG 146

[Close]

BLAST of Spg003820 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 146.0 bits (367), Expect = 3.7e-35
Identity = 77/154 (50.00%), Postives = 107/154 (69.48%), Query Frame = 0


Query: 66  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEF 125
           +AE+ R+F  FD+N DG ITK+EL +SL+N+ + + + +  +M   +D NGDG +D EEF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 126 CVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEG 185
             L + +          +E DEE++  +REAF VFD++ DG ITVEEL  VL  LGLK+G
Sbjct: 63  GGLYQTIM---------EERDEEED--MREAFNVFDQNRDGFITVEELRSVLASLGLKQG 122

Query: 186 KKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           + +E+CK MI KVD+DGDGMVNF EFK+MM+ GG
Sbjct: 123 RTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGGG 145

[Close]

BLAST of Spg003820 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-34
Identity = 76/154 (49.35%), Postives = 103/154 (66.88%), Query Frame = 0


Query: 66  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEF 125
           + E+ RVF  FDKN DG I K EL +  K++ ++V E E  EM   +D NGDG +D +EF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 126 CVLGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEG 185
             L +++            E++E+E  +REAF VFD++ DG IT EEL  VL  +GLK+G
Sbjct: 63  GSLYQEM-----------VEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQG 122

Query: 186 KKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           + +E+CK+MI KVD+DGDGMVNF EFK+MMR GG
Sbjct: 123 RTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGGG 145

[Close]

BLAST of Spg003820 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 141.4 bits (355), Expect = 9.2e-34
Identity = 72/151 (47.68%), Postives = 104/151 (68.87%), Query Frame = 0


Query: 68  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEFCV 127
           E+ RVF  FDK+ DG IT KEL ES KN+ +++ E E  ++ + +D NGDG +D EEF  
Sbjct: 5   ELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGE 64

Query: 128 LGEKLEMRRGVGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKK 187
           L + + +        ++EDE  E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK 
Sbjct: 65  LYKTIMV--------EDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKT 124

Query: 188 VENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
           +E C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 125 LEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of Spg003820 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.8 bits (351), Expect = 2.7e-33
Identity = 90/201 (44.78%), Postives = 124/201 (61.69%), Query Frame = 0


Query: 22  FLFYFPTKRFSSWLQSLKPTSQIPQHFKSSPAIAASPPPPPADAKAE----MKRVFGTFD 81
           FL Y     F+S+L  L P  ++   F  S  I    PPPP +++ E    +KRVF  FD
Sbjct: 5   FLLY---NLFNSFLLCLVP-KKLRVFFPPSWYIDDKNPPPPDESETESPVDLKRVFQMFD 64

Query: 82  KNSDGFITKKELMESLKNIRMLVTEGEAEEMFRGVDENGDGLIDFEEFCVLGEKLEMRRG 141
           KN DG ITK+EL +SL+N+ + + + +  +M + +D NGDG +D  EF    E L     
Sbjct: 65  KNGDGRITKEELNDSLENLGIFMPDKDLIQMIQKMDANGDGCVDINEF----ESLY---- 124

Query: 142 VGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKKVENCKEMIRK 201
               G   +E++E  +R+AF VFD+D DG ITVEEL+ V+  LGLK+GK +E CKEMI +
Sbjct: 125 ----GSIVEEKEEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTLECCKEMIMQ 184

Query: 202 VDLDGDGMVNFDEFKRMMRSG 219
           VD DGDG VN+ EF +MM+SG
Sbjct: 185 VDEDGDGRVNYKEFLQMMKSG 189

The following BLAST results are available for this feature:

BLAST of Spg003820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889113.1	1.8e-79	73.09	calmodulin-like protein 3 [Benincasa hispida]	[more]
XP_023545557.1	2.6e-75	70.59	calmodulin-like protein 7 [Cucurbita pepo subsp. pepo]	[more]
KAG6599380.1	7.7e-75	69.47	Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022946838.1	1.0e-74	69.47	calmodulin-like protein 7 [Cucurbita moschata]	[more]
XP_022999398.1	1.3e-74	70.32	calmodulin-like protein 7 [Cucurbita maxima]	[more]

BLAST of Spg003820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNE7	1.8e-34	50.00	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SRR7	5.2e-34	50.00	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9SU00	2.0e-33	49.35	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9ZR02	1.3e-32	47.68	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]
Q9LX27	3.8e-32	44.78	Calmodulin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=CML4 PE=2 SV=1	[more]

BLAST of Spg003820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4Z1	4.9e-75	69.47	calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=...	[more]
A0A6J1KJL5	6.4e-75	70.32	calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1	[more]
A0A0A0LL19	2.0e-73	69.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1	[more]
A0A1S3C5X4	3.5e-73	69.86	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1	[more]
A0A6J1CMI5	5.1e-64	64.19	calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV...	[more]

BLAST of Spg003820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05990.1	1.3e-35	50.00	EF hand calcium-binding protein family	[more]
AT3G07490.1	3.7e-35	50.00	ARF-GAP domain 11	[more]
AT4G12860.1	1.4e-34	49.35	EF hand calcium-binding protein family	[more]
AT4G03290.1	9.2e-34	47.68	EF hand calcium-binding protein family	[more]
AT3G59440.1	2.7e-33	44.78	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 152..180 e-value: 0.0058 score: 25.8 coord: 68..96 e-value: 8.5E-4 score: 28.6 coord: 190..218 e-value: 3.3E-6 score: 36.6 coord: 104..132 e-value: 1.1E-5 score: 34.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 152..216 e-value: 1.5E-12 score: 47.8 coord: 68..126 e-value: 1.8E-12 score: 47.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 64..99 score: 12.588623
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 148..183 score: 13.843916
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 100..135 score: 12.811786
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 186..221 score: 14.931837
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 152..216 e-value: 1.66161E-16 score: 69.1137
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 68..126 e-value: 2.98834E-15 score: 66.0321
None	No IPR available	GENE3D	1.10.238.10		coord: 29..135 e-value: 4.3E-19 score: 71.2
None	No IPR available	GENE3D	1.10.238.10		coord: 149..217 e-value: 1.2E-20 score: 75.5
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 6..222
None	No IPR available	PANTHER	PTHR10891:SF796	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 6..222
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 113..125
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 77..89
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 199..211
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 161..173
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 53..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003820.1	Spg003820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding