Spg003758 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003758
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	kunitz trypsin inhibitor 1-like
Location	scaffold4: 45084021 .. 45085277 (+)
RNA-Seq Expression	Spg003758
Synteny	Spg003758

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGTAACATCTAAACTTCATCTATACCTCTTGACTTTCTTGTCCAACTGCAAATCTTAACCATTTTGTCATCAAATCTTTCCTAAGAGGTTCCCTTATGGAACTACAAAAAATATATAATATATTTTTTTAAGTAATAAAAGCCTTAAGGTACAAGTAATGGAGAAAATCAGATAAATTAAAATTACAAAATGGACTTTTGGTCATTTTAATCTCCTTCAAGCTATTGTCAAAACAAAAAAAAAAAAAAAAAAAAAAAAAATCCTTCAAGCTGGCTATAAATGGCTCTTCACATTTGCATCCCAAACCATCATTTTTCAGACAAATTAAATTTCTAGTTGAAGAATAGAAAATGATGTTGCATAAGCCCCTTGCCATCTTCAGCTGCCTATGCATCTTCATGGCCATATCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGATCTCTACAGCGCGGCGTCGAGTACTTCATCAAGCCCGCCATCACCGATGTTGCTGGCAATCTCACCCTAGTAACTCGCAAAGGTGATCCGTGCCCGTTCCACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTATATTAAACTATTACGATTAAACTCTAGAACGAAAAATTTAAATGGATACGTTATGATATATTGAAATTTTGGACTTTTGTATGTATTCTTAACAATAGGTTTCGCAGTCACCTTCACACCCTATATAGAAGGTGAAGATACGATTAGAGAAGGGAGAGATTTGAAGGTCGTGTTTCAGGCGTTCACAACTTGCATAACGTCGACTGAATGGAGAGTAGGGGAGACGGAGCCTACCACCGGAAGGAGGTTTGTGGAGACCGGGAGCGACGACACTGCCACCGGGTACTTCAGGATCGACAAGAGCGACGGAATTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTATTGGCTTTGGATGCCTCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTAAGCCAAGAAAAGAACTTGATTTTATCTTGCAAGAGAAGATTTGTGCAATTTGTTGCAATTTGCACCAAATTAAATTAATTAAATAAAACTCTTGTAAAGGTTTTACTTTATGTTATTTATTTTTATGATTAATGTGATTTAGAAATTTGGATTGTCATCGAATATGTATTTTGTGTGTGTTTTTGTTAAGTGAATCCAATTATTT

mRNA sequence

ATGATGTTGCATAAGCCCCTTGCCATCTTCAGCTGCCTATGCATCTTCATGGCCATATCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGATCTCTACAGCGCGGCGTCGAGTACTTCATCAAGCCCGCCATCACCGATGTTGCTGGCAATCTCACCCTAGTAACTCGCAAAGGTGATCCGTGCCCGTTCCACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTTTCGCAGTCACCTTCACACCCTATATAGAAGGTGAAGATACGATTAGAGAAGGGAGAGATTTGAAGGTCGTGTTTCAGGCGTTCACAACTTGCATAACGTCGACTGAATGGAGAGTAGGGGAGACGGAGCCTACCACCGGAAGGAGGTTTGTGGAGACCGGGAGCGACGACACTGCCACCGGGTACTTCAGGATCGACAAGAGCGACGGAATTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTATTGGCTTTGGATGCCTCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTAA

Coding sequence (CDS)

ATGATGTTGCATAAGCCCCTTGCCATCTTCAGCTGCCTATGCATCTTCATGGCCATATCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGATCTCTACAGCGCGGCGTCGAGTACTTCATCAAGCCCGCCATCACCGATGTTGCTGGCAATCTCACCCTAGTAACTCGCAAAGGTGATCCGTGCCCGTTCCACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTTTCGCAGTCACCTTCACACCCTATATAGAAGGTGAAGATACGATTAGAGAAGGGAGAGATTTGAAGGTCGTGTTTCAGGCGTTCACAACTTGCATAACGTCGACTGAATGGAGAGTAGGGGAGACGGAGCCTACCACCGGAAGGAGGTTTGTGGAGACCGGGAGCGACGACACTGCCACCGGGTACTTCAGGATCGACAAGAGCGACGGAATTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTATTGGCTTTGGATGCCTCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTAA

Protein sequence

MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWRVGETEPTTGRRFVETGSDDTATGYFRIDKSDGIYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDASAFPFEFVRA

Homology

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BLAST of Spg003758 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 360.9 bits (925), Expect = 7.0e-96
Identity = 173/199 (86.93%), Postives = 182/199 (91.46%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MM HK LAIFS LC FMAISS AQ DGPPVLDTEGR LQR VEYFIKPAITDVAGNLTL+
Sbjct: 2   MMFHKSLAIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61

Query: 61  TRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPF+VGQVPLLSQDIGF VTFTPY+ GEDTIREGRDLKVVFQAFT CITST+W+
Sbjct: 62  TRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQWK 121

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGE +P TGRRFV+ G+DDTATGYFRIDKSD G+YNIGWCPSDVPIRGRPRCGSAGILIE
Sbjct: 122 VGEADPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIE 181

Query: 181 KGLRLLALDASAFPFEFVR 199
           KGLRLLALD SAFPFEFVR
Sbjct: 182 KGLRLLALDGSAFPFEFVR 200

[Close]

BLAST of Spg003758 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 340.1 bits (871), Expect = 1.3e-89
Identity = 166/200 (83.00%), Postives = 177/200 (88.50%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LC+ MA+SS AQS  PPVLDTEGR LQ  VEYFIKPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPFHVGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGET+PTTGRRFVETG DDTATGYFRID+ + G+YNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGLRLLALDASAFPFEFVRA 200
           KGLRLLALD SAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Spg003758 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 339.0 bits (868), Expect = 2.8e-89
Identity = 167/200 (83.50%), Postives = 177/200 (88.50%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LC+ MA+SS AQS  PPVLDTEGR LQ  VEYFIKPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAITDVAGNLTLL 60

Query: 61  TRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPFHVGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGET+PTTGRRFVETG +DTATGYFRID+ + GIYNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGLRLLALDASAFPFEFVRA 200
           KGLRLLALD SAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Spg003758 vs. NCBI nr
Match: XP_022150807.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 338.6 bits (867), Expect = 3.7e-89
Identity = 163/200 (81.50%), Postives = 179/200 (89.50%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           M+L KPLAIFSCL +FMAI STAQS  PPVLDT+GR LQRGVEYFIKPA+TDVAGNLTL+
Sbjct: 3   MLLQKPLAIFSCLFLFMAIYSTAQS--PPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 62

Query: 61  TRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGDPCPFHVGQVPL S DIG+ +    ++ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 63  TRKGDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWR 122

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGETEPTTGRR V TG+DDTATG+FRIDKSD G+YN+GWCPSDVPIRGRPRCG AGIL+E
Sbjct: 123 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 182

Query: 181 KGLRLLALDASAFPFEFVRA 200
           KG+RLLALD SAFPFEFVRA
Sbjct: 183 KGVRLLALDGSAFPFEFVRA 200

[Close]

BLAST of Spg003758 vs. NCBI nr
Match: KAG7021121.1 (hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.6 bits (867), Expect = 3.7e-89
Identity = 165/199 (82.91%), Postives = 176/199 (88.44%), Query Frame = 0


Query: 2   MLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVT 61
           ML K L  FS LC+ MA+SS AQS  PPVLDTEGR LQ  VEYFIKPA+TDVAGNLTL+T
Sbjct: 1   MLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLT 60

Query: 62  RKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWRV 121
           RKGD CPFHVGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WRV
Sbjct: 61  RKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRV 120

Query: 122 GETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIEK 181
           GET+PTTGRRFVETG DDTATGYFRID+ + G+YNIGWCPSDVPIRGRPRCG AGILIEK
Sbjct: 121 GETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEK 180

Query: 182 GLRLLALDASAFPFEFVRA 200
           GLRLLALD SAFPFEFVRA
Sbjct: 181 GLRLLALDGSAFPFEFVRA 197

[Close]

BLAST of Spg003758 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 6.2e-39
Identity = 88/175 (50.29%), Postives = 114/175 (65.14%), Query Frame = 0


Query: 30  VLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGDPCPFHVGQVPLLSQDIGFAVTFTP 89
           V+DT G  ++   +YFI+PAIT   G+LTLVTR  + CPF+VG  P   Q  GFAV  +P
Sbjct: 27  VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNVGLDPDAPQ--GFAVLLSP 86

Query: 90  YIEG--EDTIREGRDLKVVFQAFTTCITSTEWRVGETEPTTGRRFVETGSDDTATG---- 149
           ++    ED +R GRDL+V+FQA T+C  STEWR+GE + TTGRRF+ TG DD+  G    
Sbjct: 87  FVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITGRDDSTVGSYGN 146

Query: 150 YFRI--DKSDGIYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDASAFPFEF 197
           +FRI    S GI+NI WCP++V    +  CG+ GI+ E G  LLALD SA P  F
Sbjct: 147 FFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGSALPVAF 197

[Close]

BLAST of Spg003758 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.9e-33
Identity = 82/195 (42.05%), Postives = 113/195 (57.95%), Query Frame = 0


Query: 9   IFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGDPCP 68
           I + + + MA +S AQ     V+DT G  ++   EYFI+PAIT   G  TLVT  G PCP
Sbjct: 10  ILAHVWLLMATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-PCP 69

Query: 69  FHVGQVPLLSQDIGFAVTFTPYI--EGEDTIREGRDLKVVFQAFTTCITSTEWRVGETEP 128
            HVG +      +G AV FTP+     +D +R  RDL+V F   T+C  ST+WR+GE + 
Sbjct: 70  LHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDA 129

Query: 129 TTGRRFVETGSDDTA---TGYFRI--DKSDGIYNIGWCPSDVPIRGRPRCGSAGILIEKG 188
           T+GRR + TG D+ A     +FRI   ++ G YNI WCP++     + +CG+ G++ E G
Sbjct: 130 TSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENG 189

Query: 189 LRLLALDASAFPFEF 197
             LLALD  A P  F
Sbjct: 190 KNLLALDGDALPVVF 198

[Close]

BLAST of Spg003758 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.9e-33
Identity = 79/200 (39.50%), Postives = 116/200 (58.00%), Query Frame = 0


Query: 5   KPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKG 64
           + L I + +C+F+  ++ AQ     VLDT G  ++   EYFI+P IT+  G  T+V+R  
Sbjct: 8   RTLIILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-N 67

Query: 65  DPCPFHVGQVPLLSQDIGFAVTFTPYIEGE--DTIREGRDLKVVFQAFTTCITSTEWRVG 124
           + CP HVG + L     G  V FTP+      D +R  RDL++ FQA ++C+ STEWR+G
Sbjct: 68  ESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLG 127

Query: 125 ETEPTTGRRFVETGSDDTATG----YFRIDKS--DGIYNIGWCPSDVPIRGRPRCGSAGI 184
           E +  +GRR + TG+D    G    +FRI ++  +G+YNI WCP++V    +  CG+  +
Sbjct: 128 EKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDM 187

Query: 185 LIEKGLRLLALDASAFPFEF 197
           L E G  LLALD    P  F
Sbjct: 188 LNENGKILLALDGGPLPLVF 201

[Close]

BLAST of Spg003758 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 94.4 bits (233), Expect = 1.6e-18
Identity = 67/190 (35.26%), Postives = 93/190 (48.95%), Query Frame = 0


Query: 21  STAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTL--VTRKGD-PCPFHVGQVPLL 80
           S A S   PVLD +G  L+ G  Y+I P + D  G LT+   T  G   CP  V Q    
Sbjct: 27  SAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQT-RK 86

Query: 81  SQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTC--ITSTEWRVGETEPTTGRRFVET 140
             D    + F P    ED +R   DL + F AF  C   +ST WR+ + + +TG+ FV  
Sbjct: 87  EVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTI 146

Query: 141 G------SDDTATGYFRIDK--SDGIYNIGWCPSDVPIRGRPRCGSAGILI-EKGLRLLA 197
           G        +T + +F+I++    G Y + +CP+ V    + +CG  GI I +KG R LA
Sbjct: 147 GGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT-VCGSCKVKCGDVGIYIDQKGRRRLA 206

[Close]

BLAST of Spg003758 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 1.4e-17
Identity = 66/180 (36.67%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 3   LHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTR 62
           L  PL + S L    AIS +  +  PPV DTEG  L     Y++ PA     G LT+  R
Sbjct: 4   LRLPLILLSLL----AISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPR 63

Query: 63  KGDPCPFHVGQVPLLSQDIGFAVTFTPY----IEGEDTIREGRDLKVVFQAFTTCITSTE 122
              PCP  V Q     +  GF V FTP+       + TIR   D+++ F A T C+ STE
Sbjct: 64  V-LPCPLLVAQ-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTE 123

Query: 123 WRVGETEPTTGRRFVETG-----SDDTATGYFRIDKSDGIYNIGWCPSDVPIRGRPRCGS 174
           W VG+ EP TG R V TG     S       FR++K  G Y +  C       G  R G+
Sbjct: 124 WHVGD-EPLTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLVSCRDSCQDLGVSRDGA 176

[Close]

BLAST of Spg003758 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.2e-90
Identity = 166/200 (83.00%), Postives = 177/200 (88.50%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LC+ MA+SS AQS  PPVLDTEGR LQ  VEYFIKPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPFHVGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGET+PTTGRRFVETG DDTATGYFRID+ + G+YNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGLRLLALDASAFPFEFVRA 200
           KGLRLLALD SAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Spg003758 vs. ExPASy TrEMBL
Match: A0A6J1DBS1 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.8e-89
Identity = 163/200 (81.50%), Postives = 179/200 (89.50%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           M+L KPLAIFSCL +FMAI STAQS  PPVLDT+GR LQRGVEYFIKPA+TDVAGNLTL+
Sbjct: 3   MLLQKPLAIFSCLFLFMAIYSTAQS--PPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 62

Query: 61  TRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGDPCPFHVGQVPL S DIG+ +    ++ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 63  TRKGDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWR 122

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGETEPTTGRR V TG+DDTATG+FRIDKSD G+YN+GWCPSDVPIRGRPRCG AGIL+E
Sbjct: 123 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 182

Query: 181 KGLRLLALDASAFPFEFVRA 200
           KG+RLLALD SAFPFEFVRA
Sbjct: 183 KGVRLLALDGSAFPFEFVRA 200

[Close]

BLAST of Spg003758 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.4e-88
Identity = 159/200 (79.50%), Postives = 176/200 (88.00%), Query Frame = 0


Query: 1   MMLHKPL-AIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTL 60
           M  HK L A+FS LC FMAI STAQ DGPPVLDTEGR LQR VEYFIKPAITDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  VTRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEW 120
           +TRKGD CPF+VGQVPLLSQ+IGF VT TPY+EG+DTIREGRDLK VFQAFT CIT T+W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILI 180
           +VGE +P +GRRFV+ G+D+TATGYFRIDKS+ G+YNIGWCPSDVP +GRPRCGSAGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDASAFPFEFVR 199
           EKG+R LALD  AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of Spg003758 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.4e-86
Identity = 162/200 (81.00%), Postives = 172/200 (86.00%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCIFMAISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LC+ MA SS AQS  PPVLD EGR L+  VEYFIKPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLL 60

Query: 61  TRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPFHVGQVPL SQ+IGF VTFTPY+  ED IREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGE +PTTGRRFVETG DDTATGYFRID+ + G+YNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGLRLLALDASAFPFEFVRA 200
           KGLRLLALD SAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Spg003758 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.2e-85
Identity = 159/201 (79.10%), Postives = 172/201 (85.57%), Query Frame = 0


Query: 1   MMLHKPL-AIFSCLCIFMAISSTA-QSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLT 60
           M  HK L +IFS LC FMAI STA Q DGPPVLDTEGR LQR  EYFIKPAITDVAGNLT
Sbjct: 2   MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 61

Query: 61  LVTRKGDPCPFHVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTE 120
           L+TRKGD CPF+VGQVPLLSQ+ GFAV+  PY EGEDTIREGRDLK VFQ FT CIT T+
Sbjct: 62  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 121

Query: 121 WRVGETEPTTGRRFVETGSDDTATGYFRIDKSD-GIYNIGWCPSDVPIRGRPRCGSAGIL 180
           W+VGE +P TGRRFV+ G D+TATGYFRIDKSD G+YNIGWCPSDVPI+GRPRCGSAGIL
Sbjct: 122 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 181

Query: 181 IEKGLRLLALDASAFPFEFVR 199
           IEKG+R LALD  AFPFEFVR
Sbjct: 182 IEKGVRFLALDGPAFPFEFVR 202

[Close]

BLAST of Spg003758 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 81.3 bits (199), Expect = 9.9e-16
Identity = 58/179 (32.40%), Postives = 83/179 (46.37%), Query Frame = 0


Query: 30  VLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGDPCPFHVGQVPLLSQDIGFAVTFTP 89
           V+D +G ++     Y++ P I    G LTL  R G PCP+ + Q      D G  V F+ 
Sbjct: 30  VVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ-ESSEVDEGIPVKFSN 89

Query: 90  YIEGEDTIREGRDLKVVFQ-AFTTCITSTEWRVGETEPTTGRRFVETG------SDDTAT 149
           +      + E ++L +      T CI ST WRVGE +    + FV  G        D+  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 150 GYFRIDKS-DGIYNIGWCPSDVPIRGRPRCGSAGILI-EKGLRLLALDASAFPFEFVRA 200
            +F+I+KS +  Y   +CP      G P+C   GI I E G+R LAL    F   F +A
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKA 205

[Close]

BLAST of Spg003758 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.4e-14
Identity = 62/201 (30.85%), Postives = 91/201 (45.27%), Query Frame = 0


Query: 7   LAIFSCLCIFMAISS-TAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGD 66
           L IF  L +F++    T ++   PV D  G+SL  GV Y+I P I    G LT+   K +
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 67  PCPFHVGQVPL-LSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWRVGET 126
            CP  V Q    +SQ  G  V F+PY +   TI    D+ + F        ++ W +   
Sbjct: 65  TCPTSVIQDQFEVSQ--GLPVKFSPY-DKSRTIPVSTDVNIKFS------PTSIWELANF 124

Query: 127 EPTTGRRFVET------GSDDTATGYFRIDKSDGIYNIGWCPSDVPIRGRPRCGSAGILI 186
           + TT + F+ T          T   +F+IDK +  Y I +CP+ V    +  C   G+ +
Sbjct: 125 DETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPT-VCNFCKVICRDVGVFV 184

Query: 187 EKGLRLLALDASAFPFEFVRA 200
           + G R LAL        F RA
Sbjct: 185 QDGKRRLALSDVPLKVMFKRA 195

The following BLAST results are available for this feature:

BLAST of Spg003758 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888505.1	7.0e-96	86.93	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]
XP_022938134.1	1.3e-89	83.00	alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata]	[more]
XP_023521354.1	2.8e-89	83.50	alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381...	[more]
XP_022150807.1	3.7e-89	81.50	miraculin-like [Momordica charantia]	[more]
KAG7021121.1	3.7e-89	82.91	hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Spg003758 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	6.2e-39	50.29	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	3.9e-33	42.05	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV1	3.9e-33	39.50	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
P13087	1.6e-18	35.26	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P29421	1.4e-17	36.67	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of Spg003758 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FD70	6.2e-90	83.00	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1DBS1	1.8e-89	81.50	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1	[more]
A0A0A0LGB4	4.4e-88	79.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A6J1HLP6	1.4e-86	81.00	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]
A0A1S3C0M7	1.2e-85	79.10	endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO...	[more]

BLAST of Spg003758 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	9.9e-16	32.40	kunitz trypsin inhibitor 1	[more]
AT1G17860.1	1.4e-14	30.85	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 29..199 e-value: 1.2E-25 score: 101.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 30..197 e-value: 1.7E-37 score: 129.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 7..199
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 30..46
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 29..198 e-value: 1.43382E-36 score: 123.606
None	No IPR available	GENE3D	2.80.10.50		coord: 27..199 e-value: 1.6E-40 score: 140.6
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 7..199
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003758.1	Spg003758.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0050896	response to stimulus
molecular_function	GO:0004866	endopeptidase inhibitor activity