Spg003755 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003755
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold4: 46618046 .. 46620458 (-)
RNA-Seq Expression	Spg003755
Synteny	Spg003755

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCGTTTCATCAATGGCGGTTCCGAAGGGGAAATACAGCAAGTTGCAGGGCAAGAAATGGTCCACATTCCAGCTTTCCAAGATGATTATGGCCCTCGTTTTGGTACTTGGGTTTTCCATGCTTCTCGCTCTCCGATTCTTCTCTCCTCCTGAAAGTTCTCATCGGAATCTACCCGACCGTCTCGCCTCCGTCCAGCATAGAGCCGTTCAAAGGTACTCCGGCAATGTTTCTGATTGTTTGAACTTGTTAGCGGAATCAAGTGACTTTGATTTTGATTTTGGTACTTTTTCTTTTTTTGAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCATGGGAGCCTAGAGCTTTCGTTTATCACAATTTCTTGGTAAGTTTTTAGAATTGTGGTTTCTTTTTGGCTTTTTGTTTCATTTTGTTATGTTTTGTTTGAAATGTTGTCTGATTTGGGAATGCTGTTGGACAGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAGCCCCACATGGTGAAATCAACTGTGGTTGATAGCAAAACGGGCAAGAGTGTAGATAGCAGGTCTGTATCTGTCATTTTCATGTTTGTCTCTTATAGAGCTTGACAGTTATGGCTATGAGTATGAACTAAATTTGGTGGGGCTTACACTTCACACATCTATAGTGGTTTTTTGGAAAAATTCTCAAAGAGAGAGGTTAGTTTTGAAATACTCTTATCTTCTAAAGATGATTTATCTGCAAATAGTCTTTTTGATCAATTCTCTCTGAAAAAAAACATATTTGTGGGAGATAAATCCCAAAAGTGGCTAAAGCCAAAACAGGAAAAGAAAAAAAAATGAAGCTGAAGTTTGGTTGATTCGAAGTATTACTTTTTTTTTTTTTTTTTTTTAGTTAAAAAACATTTGGGGTAGGGGATTCGAACCTTTGACCTCTTGGTCAAGATACGTGCCAATTTCCGCTTAGTTATGCTCGCTTTGGCTTGATTTGAAGTATACTTGTTAGTTGGTTCGGTTTTTTAAACTCCAAAGACTGAAAATAGCCAGTTCAAATAGATTTTGTTGGTTTGATTGGTTTGAGTGTTAGATGGCTAGTTTGGTCTGGTCGGGTCCGTTTTTTAAGAAATCGATTTGGTAACAGACTCACCATAATAAAATGGCAATGTGCAAAGTATAAAAGAAGGGTATAACAATACAATCACAATAAACAAGGCAAACTTATATTTATTTGCTTACAATGTCATGTCCACAGGGTGCGCACCAGTTCTGGAATGTTTCTGAATAGAGGGCATGACAAAATCATCAGTAACATTGAGAAAAGAATAGCAGATTTCACATTCATTCCTATAGGTAAAGATTTCCGAAACTTACCTATTATTACCTTGGCTAATTCCTCATGCATTTTGTAACAATTCCTATCTATAGGGCTTGTTTGGTAGTACTTCTCAAAACAGTTTTTGAAAACATGTCTGATTCACTGTTTTACAAAAGTTGTTTTTTATCTGTTAGTCTTGTCAAGTGGTACAAGATAGTGATTTCATTGTATTCAATAACATCAATCTTTCAAGGCATTCAATTATAGTTCTGTTGAATGGTTCTTAAAAAACAGAGTTGCAACAGAACATAATTCTCATACAAAATTTGGTATCAGACTAGTCAAATATTGATGAAAAATTAGCTAAATTTCATGTGGGTTTGTTCTACATTCTTGTTTTAAGGCCCAAACCAAACAGGGCTTTGGTGAACAACTCATTCATTTGATTGTTGATGCTTTGTTGATGTCACTGACATTCTTGGTCTTTACACATTTTGGTATTAATGTTGATATTCACACATTCAAACAGATCATGAAGAATGTGTGTTTGTTCTCCATTCTACTTTTGAGGTTCAAACCAAACAGGGATTTATCAAACACTATTCATCTTCTTGTTGATGTCACTGACATTCTTGTTCTTAACACATTTTGATATTAATGTTGATATTCACTATTCCAAACAGAACATGGAGAAGGACTTCAAATTCTGCACTATGAAGTTGGGCAAAAGTATGATGCTCACTACGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTCCTCATGTATCTGTAAGCTTTGACATCTCTCAAAACTCATCTACCCCTCTCTCTAGTTCTCGCTCTGAATTGCTACCGATACGAATGCAGGTCGGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCTGCTGCTCAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTGTCTGAATGTGGTAAAGGTGGACTCTCGGTAAAACCAAAGATGGGAGATGCTTTATTGTTCTGGAGCATGAATCCTGATACTACCTTAGATCCTACAAGTTTGCATGGTTAG

mRNA sequence

ATGTTCGTTTCATCAATGGCGGTTCCGAAGGGGAAATACAGCAAGTTGCAGGGCAAGAAATGGTCCACATTCCAGCTTTCCAAGATGATTATGGCCCTCGTTTTGGTACTTGGGTTTTCCATGCTTCTCGCTCTCCGATTCTTCTCTCCTCCTGAAAGTTCTCATCGGAATCTACCCGACCGTCTCGCCTCCGTCCAGCATAGAGCCGTTCAAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCATGGGAGCCTAGAGCTTTCGTTTATCACAATTTCTTGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAGCCCCACATGGTGAAATCAACTGTGGTTGATAGCAAAACGGGCAAGAGTGTAGATAGCAGGGTGCGCACCAGTTCTGGAATGTTTCTGAATAGAGGGCATGACAAAATCATCAGTAACATTGAGAAAAGAATAGCAGATTTCACATTCATTCCTATAGAACATGGAGAAGGACTTCAAATTCTGCACTATGAAGTTGGGCAAAAGTATGATGCTCACTACGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTCCTCATGTATCTGTCGGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCTGCTGCTCAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTGTCTGAATGTGGTAAAGGTGGACTCTCGGTAAAACCAAAGATGGGAGATGCTTTATTGTTCTGGAGCATGAATCCTGATACTACCTTAGATCCTACAAGTTTGCATGGTTAG

Coding sequence (CDS)

ATGTTCGTTTCATCAATGGCGGTTCCGAAGGGGAAATACAGCAAGTTGCAGGGCAAGAAATGGTCCACATTCCAGCTTTCCAAGATGATTATGGCCCTCGTTTTGGTACTTGGGTTTTCCATGCTTCTCGCTCTCCGATTCTTCTCTCCTCCTGAAAGTTCTCATCGGAATCTACCCGACCGTCTCGCCTCCGTCCAGCATAGAGCCGTTCAAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCATGGGAGCCTAGAGCTTTCGTTTATCACAATTTCTTGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAGCCCCACATGGTGAAATCAACTGTGGTTGATAGCAAAACGGGCAAGAGTGTAGATAGCAGGGTGCGCACCAGTTCTGGAATGTTTCTGAATAGAGGGCATGACAAAATCATCAGTAACATTGAGAAAAGAATAGCAGATTTCACATTCATTCCTATAGAACATGGAGAAGGACTTCAAATTCTGCACTATGAAGTTGGGCAAAAGTATGATGCTCACTACGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTCCTCATGTATCTGTCGGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCTGCTGCTCAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTGTCTGAATGTGGTAAAGGTGGACTCTCGGTAAAACCAAAGATGGGAGATGCTTTATTGTTCTGGAGCATGAATCCTGATACTACCTTAGATCCTACAAGTTTGCATGGTTAG

Protein sequence

MFVSSMAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASVQHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTGKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMNPDTTLDPTSLHG

Homology

[Close]

BLAST of Spg003755 vs. NCBI nr
Match: XP_038889689.1 (probable prolyl 4-hydroxylase 3 [Benincasa hispida])

HSP 1 Score: 514.6 bits (1324), Expect = 5.1e-142
Identity = 248/266 (93.23%), Postives = 256/266 (96.24%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MA+ KGKY+K+QGKKWSTF+LSKMIMALVL LGF MLLALRFFSPPE+SHRNLP  LASV
Sbjct: 1   MALSKGKYTKIQGKKWSTFELSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHHLASV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +H AV+SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RHSAVESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKSVDSRVRTSSGMFLNRG DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAARGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 266

[Close]

BLAST of Spg003755 vs. NCBI nr
Match: XP_008456388.1 (PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo])

HSP 1 Score: 499.2 bits (1284), Expect = 2.2e-137
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MAV KGKY KLQGKKWSTFQLSKMIMALVL LGF ML+ALRFFSPPE+SH  LP    SV
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP----SV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +  A QSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKSVDSRVRTSSGMFLNRG DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 262

[Close]

BLAST of Spg003755 vs. NCBI nr
Match: KAA0063574.1 (putative prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo var. makuwa] >TYJ97902.1 putative prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 499.2 bits (1284), Expect = 2.2e-137
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MAV KGKY KLQGKKWSTFQLSKMIMALVL LGF ML+ALRFFSPPE+SH  LP    SV
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP----SV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +  A QSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKSVDSRVRTSSGMFLNRG DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 262

[Close]

BLAST of Spg003755 vs. NCBI nr
Match: XP_008456383.1 (PREDICTED: probable prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo])

HSP 1 Score: 499.2 bits (1284), Expect = 2.2e-137
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MAV KGKY KLQGKKWSTFQLSKMIMALVL LGF ML+ALRFFSPPE+SH  LP    SV
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP----SV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +  A QSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKSVDSRVRTSSGMFLNRG DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 262

[Close]

BLAST of Spg003755 vs. NCBI nr
Match: XP_011648735.2 (probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical protein Csa_019368 [Cucumis sativus])

HSP 1 Score: 492.7 bits (1267), Expect = 2.1e-135
Identity = 240/266 (90.23%), Postives = 248/266 (93.23%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MA+ KGKY KLQG+KWSTFQLSKMIMALVL LGF ML+ALRFFSPPE+SH     R +SV
Sbjct: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHH----RFSSV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +H A  SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           G+SVDSRVRTSSGMFLNRG DKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 262

[Close]

BLAST of Spg003755 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.1e-106
Identity = 190/263 (72.24%), Postives = 214/263 (81.37%), Query Frame = 0


Query: 10  KGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASVQHRA 69
           K ++S+ Q +KWST  L  + M  +L +   MLLA   FS P ++  + P  L+  +  A
Sbjct: 3   KLRHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAA 62

Query: 70  VQ-SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTGKS 129
            + S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHMVKSTVVDS+TGKS
Sbjct: 63  TERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKS 122

Query: 130 VDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYF 189
            DSRVRTSSG FL RG DKII  IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYDYF
Sbjct: 123 KDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYF 182

Query: 190 VDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVKPK 249
           VDE+N K GGQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVKP+
Sbjct: 183 VDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPR 242

Query: 250 MGDALLFWSMNPDTTLDPTSLHG 272
           MGDALLFWSM PD TLDPTSLHG
Sbjct: 243 MGDALLFWSMRPDATLDPTSLHG 264

[Close]

BLAST of Spg003755 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-97
Identity = 175/268 (65.30%), Postives = 207/268 (77.24%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFS-PPESSHRNLPDRLAS 65
           MA P+      +    ST   + +IM+  ++L   +LLA    S P  ++  +  + L S
Sbjct: 2   MARPRNHRPSARKSSHSTLVFAVLIMSTFVIL---ILLAFGILSVPSNNAGSSKANDLTS 61

Query: 66  VQHRAVQSDGL-GKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDS 125
           +  + +Q  G    + ++WVE ISWEPRA VYHNFL+KEEC YLI LAKPHM KSTVVD 
Sbjct: 62  IVRKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDE 121

Query: 126 KTGKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 185
           KTGKS DSRVRTSSG FL RG DK I  IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ 
Sbjct: 122 KTGKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEP 181

Query: 186 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGL 245
           HYDYF+DEYN + GGQR+AT+LMYLSDVEEGGETVFPAA+GN+S+VPWWNELSECGKGGL
Sbjct: 182 HYDYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGL 241

Query: 246 SVKPKMGDALLFWSMNPDTTLDPTSLHG 272
           SVKPKMGDALLFWSM PD TLDP+SLHG
Sbjct: 242 SVKPKMGDALLFWSMTPDATLDPSSLHG 266

[Close]

BLAST of Spg003755 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 2.8e-90
Identity = 161/265 (60.75%), Postives = 200/265 (75.47%), Query Frame = 0


Query: 12  KYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPP-----ESSHRNLPDRLASVQ 71
           K  +L+ K   +F      + ++++    +L+ L  FS P      S   +L   + ++Q
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQ 63

Query: 72  HRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTG 131
            R    D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M+KS VVD KTG
Sbjct: 64  ERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTG 123

Query: 132 KSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD 191
           KS+DSRVRTSSG FLNRGHD+I+  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ H+D
Sbjct: 124 KSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHD 183

Query: 192 YFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVK 251
           YF DE+N++KGGQR+AT+LMYLSDV+EGGETVFPAA+GN S VPWW+ELS+CGK GLSV 
Sbjct: 184 YFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVL 243

Query: 252 PKMGDALLFWSMNPDTTLDPTSLHG 272
           PK  DALLFWSM PD +LDP+SLHG
Sbjct: 244 PKKRDALLFWSMKPDASLDPSSLHG 268

[Close]

BLAST of Spg003755 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 6.8e-89
Identity = 161/262 (61.45%), Postives = 204/262 (77.86%), Query Frame = 0


Query: 12  KYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESS-HRNLPDRLASVQHRAV 71
           +Y   +    ST   + +I+ LV++L   +LL L   S P ++ + +  + L ++  ++ 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVIL---ILLGLGILSLPNANRNSSKTNDLTNIVRKSE 69

Query: 72  QSDGLGK-RGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTGKSV 131
            S G  +  G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP MVKSTVVD KTG S 
Sbjct: 70  TSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSK 129

Query: 132 DSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 191
           DSRVRTSSG FL RGHD+++  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ HYDYF+
Sbjct: 130 DSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFL 189

Query: 192 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVKPKM 251
           DE+N K GGQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GLSV PK 
Sbjct: 190 DEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKK 249

Query: 252 GDALLFWSMNPDTTLDPTSLHG 272
            DALLFW+M PD +LDP+SLHG
Sbjct: 250 RDALLFWNMRPDASLDPSSLHG 268

[Close]

BLAST of Spg003755 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 8.4e-55
Identity = 101/186 (54.30%), Postives = 140/186 (75.27%), Query Frame = 0


Query: 86  ISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTGKSVDSRVRTSSGMFLNRGH 145
           +SW PR F+Y  FLS EEC + I LAK  + KS V D+ +G+SV+S VRTSSGMFL++  
Sbjct: 59  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 118

Query: 146 DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLL 205
           D I+SN+E ++A +TF+P E+GE +QILHYE GQKY+ H+DYF D+ N++ GG R+AT+L
Sbjct: 119 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 178

Query: 206 MYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMNPDTTLD 265
           MYLS+VE+GGETVFP  +G  + +   +  +EC K G +VKP+ GDALLF++++P+ T D
Sbjct: 179 MYLSNVEKGGETVFPMWKGKATQLK-DDSWTECAKQGYAVKPRKGDALLFFNLHPNATTD 238

Query: 266 PTSLHG 272
             SLHG
Sbjct: 239 SNSLHG 243

[Close]

BLAST of Spg003755 vs. ExPASy TrEMBL
Match: A0A5D3BDQ0 (Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001180 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.1e-137
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MAV KGKY KLQGKKWSTFQLSKMIMALVL LGF ML+ALRFFSPPE+SH  LP    SV
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP----SV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +  A QSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKSVDSRVRTSSGMFLNRG DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 262

[Close]

BLAST of Spg003755 vs. ExPASy TrEMBL
Match: A0A1S3C2P6 (probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.1e-137
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MAV KGKY KLQGKKWSTFQLSKMIMALVL LGF ML+ALRFFSPPE+SH  LP    SV
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP----SV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +  A QSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKSVDSRVRTSSGMFLNRG DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 262

[Close]

BLAST of Spg003755 vs. ExPASy TrEMBL
Match: A0A1S3C367 (probable prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.1e-137
Identity = 245/266 (92.11%), Postives = 249/266 (93.61%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MAV KGKY KLQGKKWSTFQLSKMIMALVL LGF ML+ALRFFSPPE+SH  LP    SV
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLP----SV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           +  A QSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKSVDSRVRTSSGMFLNRG DKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD TLDPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHG 262

[Close]

BLAST of Spg003755 vs. ExPASy TrEMBL
Match: A0A6J1CNS9 (probable prolyl 4-hydroxylase 3 OS=Momordica charantia OX=3673 GN=LOC111013165 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.0e-132
Identity = 233/268 (86.94%), Postives = 251/268 (93.66%), Query Frame = 0


Query: 6   MAVPKGKYSKL--QGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLA 65
           MAV KGKY K+   G+KWST +LSK+IMALVL LGFSMLLALRFFSPPESS  NLPDRLA
Sbjct: 1   MAVSKGKYGKINFHGRKWSTLELSKIIMALVLALGFSMLLALRFFSPPESSDPNLPDRLA 60

Query: 66  SVQHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDS 125
           SV+ +AV+S+GLGKRG+QWVE ISWEPRAF+YHNFLSKEECLYLISLAKP MVKSTV+DS
Sbjct: 61  SVRRKAVESEGLGKRGEQWVEVISWEPRAFIYHNFLSKEECLYLISLAKPRMVKSTVIDS 120

Query: 126 KTGKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 185
           +TGKS+DSRVRTSSGMFL+RG D+II NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA
Sbjct: 121 ETGKSMDSRVRTSSGMFLSRGQDRIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 180

Query: 186 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGL 245
           H+DYFVDEYNIKKG QRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGL
Sbjct: 181 HHDYFVDEYNIKKGSQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 240

Query: 246 SVKPKMGDALLFWSMNPDTTLDPTSLHG 272
           SVKPKMGDALLFWSM PD +LDPTSLHG
Sbjct: 241 SVKPKMGDALLFWSMKPDASLDPTSLHG 268

[Close]

BLAST of Spg003755 vs. ExPASy TrEMBL
Match: A0A6J1FBR3 (probable prolyl 4-hydroxylase 3 OS=Cucurbita moschata OX=3662 GN=LOC111444174 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 5.2e-132
Identity = 229/266 (86.09%), Postives = 246/266 (92.48%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASV 65
           MAV KGKY K Q +KWSTF+LSK+IMA +L LG SML+A RFFSPPESSH NL  R+ASV
Sbjct: 1   MAVSKGKYVKFQARKWSTFKLSKIIMAFLLALGVSMLIAFRFFSPPESSHSNLLHRVASV 60

Query: 66  QHRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKT 125
           QHRAV SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHM KSTVVD+KT
Sbjct: 61  QHRAVHSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMAKSTVVDNKT 120

Query: 126 GKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GKS+DSRVRTSSGMFL RG +KI+SNIEKRIADFTFIP+EHGE LQILHYEVGQKYDAH+
Sbjct: 121 GKSIDSRVRTSSGMFLKRGQNKIVSNIEKRIADFTFIPVEHGEALQILHYEVGQKYDAHH 180

Query: 186 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSV 245
           DYF DE+NIK+GGQRMATLLMYLSDVEEGGETVFPAA+GNFSS+P WNELSECGKGGLSV
Sbjct: 181 DYFADEFNIKQGGQRMATLLMYLSDVEEGGETVFPAAEGNFSSLPGWNELSECGKGGLSV 240

Query: 246 KPKMGDALLFWSMNPDTTLDPTSLHG 272
           KPKMGDALLFWSM PD T+DPTSLHG
Sbjct: 241 KPKMGDALLFWSMKPDNTVDPTSLHG 266

[Close]

BLAST of Spg003755 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 386.7 bits (992), Expect = 1.5e-107
Identity = 190/263 (72.24%), Postives = 214/263 (81.37%), Query Frame = 0


Query: 10  KGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESSHRNLPDRLASVQHRA 69
           K ++S+ Q +KWST  L  + M  +L +   MLLA   FS P ++  + P  L+  +  A
Sbjct: 3   KLRHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAA 62

Query: 70  VQ-SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTGKS 129
            + S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHMVKSTVVDS+TGKS
Sbjct: 63  TERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKS 122

Query: 130 VDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYF 189
            DSRVRTSSG FL RG DKII  IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYDYF
Sbjct: 123 KDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYF 182

Query: 190 VDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVKPK 249
           VDE+N K GGQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVKP+
Sbjct: 183 VDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPR 242

Query: 250 MGDALLFWSMNPDTTLDPTSLHG 272
           MGDALLFWSM PD TLDPTSLHG
Sbjct: 243 MGDALLFWSMRPDATLDPTSLHG 264

[Close]

BLAST of Spg003755 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 9.8e-99
Identity = 175/268 (65.30%), Postives = 207/268 (77.24%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFS-PPESSHRNLPDRLAS 65
           MA P+      +    ST   + +IM+  ++L   +LLA    S P  ++  +  + L S
Sbjct: 2   MARPRNHRPSARKSSHSTLVFAVLIMSTFVIL---ILLAFGILSVPSNNAGSSKANDLTS 61

Query: 66  VQHRAVQSDGL-GKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDS 125
           +  + +Q  G    + ++WVE ISWEPRA VYHNFL+KEEC YLI LAKPHM KSTVVD 
Sbjct: 62  IVRKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDE 121

Query: 126 KTGKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 185
           KTGKS DSRVRTSSG FL RG DK I  IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ 
Sbjct: 122 KTGKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEP 181

Query: 186 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGL 245
           HYDYF+DEYN + GGQR+AT+LMYLSDVEEGGETVFPAA+GN+S+VPWWNELSECGKGGL
Sbjct: 182 HYDYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGL 241

Query: 246 SVKPKMGDALLFWSMNPDTTLDPTSLHG 272
           SVKPKMGDALLFWSM PD TLDP+SLHG
Sbjct: 242 SVKPKMGDALLFWSMTPDATLDPSSLHG 266

[Close]

BLAST of Spg003755 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.0e-91
Identity = 161/265 (60.75%), Postives = 200/265 (75.47%), Query Frame = 0


Query: 12  KYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPP-----ESSHRNLPDRLASVQ 71
           K  +L+ K   +F      + ++++    +L+ L  FS P      S   +L   + ++Q
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQ 63

Query: 72  HRAVQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTG 131
            R    D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M+KS VVD KTG
Sbjct: 64  ERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTG 123

Query: 132 KSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD 191
           KS+DSRVRTSSG FLNRGHD+I+  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ H+D
Sbjct: 124 KSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHD 183

Query: 192 YFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVK 251
           YF DE+N++KGGQR+AT+LMYLSDV+EGGETVFPAA+GN S VPWW+ELS+CGK GLSV 
Sbjct: 184 YFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVL 243

Query: 252 PKMGDALLFWSMNPDTTLDPTSLHG 272
           PK  DALLFWSM PD +LDP+SLHG
Sbjct: 244 PKKRDALLFWSMKPDASLDPSSLHG 268

[Close]

BLAST of Spg003755 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 328.6 bits (841), Expect = 4.9e-90
Identity = 161/262 (61.45%), Postives = 204/262 (77.86%), Query Frame = 0


Query: 12  KYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFSPPESS-HRNLPDRLASVQHRAV 71
           +Y   +    ST   + +I+ LV++L   +LL L   S P ++ + +  + L ++  ++ 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVIL---ILLGLGILSLPNANRNSSKTNDLTNIVRKSE 69

Query: 72  QSDGLGK-RGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDSKTGKSV 131
            S G  +  G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP MVKSTVVD KTG S 
Sbjct: 70  TSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSK 129

Query: 132 DSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 191
           DSRVRTSSG FL RGHD+++  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ HYDYF+
Sbjct: 130 DSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFL 189

Query: 192 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGGLSVKPKM 251
           DE+N K GGQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GLSV PK 
Sbjct: 190 DEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKK 249

Query: 252 GDALLFWSMNPDTTLDPTSLHG 272
            DALLFW+M PD +LDP+SLHG
Sbjct: 250 RDALLFWNMRPDASLDPSSLHG 268

[Close]

BLAST of Spg003755 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 293.9 bits (751), Expect = 1.3e-79
Identity = 148/239 (61.92%), Postives = 178/239 (74.48%), Query Frame = 0


Query: 6   MAVPKGKYSKLQGKKWSTFQLSKMIMALVLVLGFSMLLALRFFS-PPESSHRNLPDRLAS 65
           MA P+      +    ST   + +IM+  ++L   +LLA    S P  ++  +  + L S
Sbjct: 2   MARPRNHRPSARKSSHSTLVFAVLIMSTFVIL---ILLAFGILSVPSNNAGSSKANDLTS 61

Query: 66  VQHRAVQSDGL-GKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMVKSTVVDS 125
           +  + +Q  G    + ++WVE ISWEPRA VYHNFL  EEC YLI LAKPHM KSTVVD 
Sbjct: 62  IVRKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDE 121

Query: 126 KTGKSVDSRVRTSSGMFLNRGHDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 185
           KTGKS DSRVRTSSG FL RG DK I  IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ 
Sbjct: 122 KTGKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEP 181

Query: 186 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAQGNFSSVPWWNELSECGKGG 243
           HYDYF+DEYN + GGQR+AT+LMYLSDVEEGGETVFPAA+GN+S+VPWWNELSECGKGG
Sbjct: 182 HYDYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:

BLAST of Spg003755 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889689.1	5.1e-142	93.23	probable prolyl 4-hydroxylase 3 [Benincasa hispida]	[more]
XP_008456388.1	2.2e-137	92.11	PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo]	[more]
KAA0063574.1	2.2e-137	92.11	putative prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo var. makuwa] >TYJ97902....	[more]
XP_008456383.1	2.2e-137	92.11	PREDICTED: probable prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo]	[more]
XP_011648735.2	2.1e-135	90.23	probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical pro...	[more]

BLAST of Spg003755 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LN20	2.1e-106	72.24	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
F4JZ24	1.4e-97	65.30	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
F4JNU8	2.8e-90	60.75	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
Q24JN5	6.8e-89	61.45	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
Q8L970	8.4e-55	54.30	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]

BLAST of Spg003755 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BDQ0	1.1e-137	92.11	Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3C2P6	1.1e-137	92.11	probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034963...	[more]
A0A1S3C367	1.1e-137	92.11	probable prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034963...	[more]
A0A6J1CNS9	1.0e-132	86.94	probable prolyl 4-hydroxylase 3 OS=Momordica charantia OX=3673 GN=LOC111013165 P...	[more]
A0A6J1FBR3	5.2e-132	86.09	probable prolyl 4-hydroxylase 3 OS=Cucurbita moschata OX=3662 GN=LOC111444174 PE...	[more]

BLAST of Spg003755 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20270.1	1.5e-107	72.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.1	9.8e-99	65.30	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	2.0e-91	60.75	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	4.9e-90	61.45	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	1.3e-79	61.92	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 90..271 e-value: 2.6E-41 score: 153.2
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 170..259 e-value: 4.5E-13 score: 49.9
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 83..271 e-value: 3.5E-68 score: 231.4
None	No IPR available	PANTHER	PTHR10869:SF169	PROLYL 4-HYDROXYLASE 3-RELATED	coord: 9..271
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 9..271
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 166..271 score: 9.136835

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003755.1	Spg003755.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen