Spg003696 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003696 vs. NCBI nr
Match: XP_038877416.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X2 [Benincasa hispida])

HSP 1 Score: 626.7 bits (1615), Expect = 1.3e-175
Identity = 304/370 (82.16%), Postives = 318/370 (85.95%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MENPT+ D NPKFLALNCIDLSSSDV++SVSLLK                          
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLK-------------------------- 87

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGITEEFM EVFAQSKRLFSLPLSDKMKLLRNEKH
Sbjct: 88  ------------QACLDTGFFYAVNHGITEEFMDEVFAQSKRLFSLPLSDKMKLLRNEKH 147

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTPILDELLDPDNQIRGDYKEGYYIG+EEK PG+  DGERPFYGPNIWPAEDLLPGW+
Sbjct: 148 RGYTPILDELLDPDNQIRGDYKEGYYIGIEEKNPGDDSDGERPFYGPNIWPAEDLLPGWR 207

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           +IMEKFH QALEVAR IARLIALAL LENDFFDK+E LGEPIA+LRLLHYEGQ+SEPAKG
Sbjct: 208 KIMEKFHTQALEVARAIARLIALALDLENDFFDKAETLGEPIAILRLLHYEGQVSEPAKG 267

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW+YVAPLKG FVVNLGDMLERWSN
Sbjct: 268 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWQYVAPLKGTFVVNLGDMLERWSN 327

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
           CIFKSTLHRVLVNGQDRYSIAYFVEP+HDCLVECLPTC S+KNPPKFPPI C TYLSQRY
Sbjct: 328 CIFKSTLHRVLVNGQDRYSIAYFVEPNHDCLVECLPTCKSDKNPPKFPPIRCRTYLSQRY 359

Query: 361 KETHVDLNTY 371
           KETHVDL+TY
Sbjct: 388 KETHVDLSTY 359

BLAST of Spg003696 vs. NCBI nr
Match: XP_023545999.1 (2-oxoglutarate-dependent dioxygenase mpl2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.1 bits (1603), Expect = 3.2e-174
Identity = 303/374 (81.02%), Postives = 317/374 (84.76%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T  DPNP F ALNCIDLSSSD+H SVSLLK                          
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGI EEFM EVFAQSKRLFSLPLSDKMKLLRNEKH
Sbjct: 61  ------------QACLDTGFFYAVNHGIPEEFMDEVFAQSKRLFSLPLSDKMKLLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTPILDELLDPDNQIRGDYKEGYYIG+EEKIPG+   GE PFYGPNIWPAEDLLPGW+
Sbjct: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDSGGEMPFYGPNIWPAEDLLPGWR 180

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           +IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG
Sbjct: 181 KIMEKFHREALEVARAIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           IFGAGAHTDYGLITLLATDEN GLQICKDK+AKPQIWEYVAPLKGAFVVNLGDMLERWSN
Sbjct: 241 IFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
           C+F+STLHRVL NGQDRYSIAYFVEPSHDCLVECLPTC S++NPPKFPPILC TYLSQRY
Sbjct: 301 CVFRSTLHRVLGNGQDRYSIAYFVEPSHDCLVECLPTCKSDENPPKFPPILCKTYLSQRY 336

Query: 361 KETHVDLNTYNNED 375
           KETHVDL+TY + +
Sbjct: 361 KETHVDLDTYKSSN 336

BLAST of Spg003696 vs. NCBI nr
Match: XP_038877418.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X4 [Benincasa hispida])

HSP 1 Score: 622.1 bits (1603), Expect = 3.2e-174
Identity = 304/371 (81.94%), Postives = 318/371 (85.71%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MENPT+ D NPKFLALNCIDLSSSDV++SVSLLK                          
Sbjct: 1   MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGITEEFM EVFAQSKRLFSLPLSDKMKLLRNEKH
Sbjct: 61  ------------QACLDTGFFYAVNHGITEEFMDEVFAQSKRLFSLPLSDKMKLLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGW 180
           RGYTPILDELLDPDNQIR GDYKEGYYIG+EEK PG+  DGERPFYGPNIWPAEDLLPGW
Sbjct: 121 RGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKNPGDDSDGERPFYGPNIWPAEDLLPGW 180

Query: 181 KEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK 240
           ++IMEKFH QALEVAR IARLIALAL LENDFFDK+E LGEPIA+LRLLHYEGQ+SEPAK
Sbjct: 181 RKIMEKFHTQALEVARAIARLIALALDLENDFFDKAETLGEPIAILRLLHYEGQVSEPAK 240

Query: 241 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWS 300
           GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW+YVAPLKG FVVNLGDMLERWS
Sbjct: 241 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWQYVAPLKGTFVVNLGDMLERWS 300

Query: 301 NCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQR 360
           NCIFKSTLHRVLVNGQDRYSIAYFVEP+HDCLVECLPTC S+KNPPKFPPI C TYLSQR
Sbjct: 301 NCIFKSTLHRVLVNGQDRYSIAYFVEPNHDCLVECLPTCKSDKNPPKFPPIRCRTYLSQR 333

Query: 361 YKETHVDLNTY 371
           YKETHVDL+TY
Sbjct: 361 YKETHVDLSTY 333

BLAST of Spg003696 vs. NCBI nr
Match: XP_038877415.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X1 [Benincasa hispida])

HSP 1 Score: 622.1 bits (1603), Expect = 3.2e-174
Identity = 304/371 (81.94%), Postives = 318/371 (85.71%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MENPT+ D NPKFLALNCIDLSSSDV++SVSLLK                          
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLK-------------------------- 87

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGITEEFM EVFAQSKRLFSLPLSDKMKLLRNEKH
Sbjct: 88  ------------QACLDTGFFYAVNHGITEEFMDEVFAQSKRLFSLPLSDKMKLLRNEKH 147

Query: 121 RGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGW 180
           RGYTPILDELLDPDNQIR GDYKEGYYIG+EEK PG+  DGERPFYGPNIWPAEDLLPGW
Sbjct: 148 RGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKNPGDDSDGERPFYGPNIWPAEDLLPGW 207

Query: 181 KEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK 240
           ++IMEKFH QALEVAR IARLIALAL LENDFFDK+E LGEPIA+LRLLHYEGQ+SEPAK
Sbjct: 208 RKIMEKFHTQALEVARAIARLIALALDLENDFFDKAETLGEPIAILRLLHYEGQVSEPAK 267

Query: 241 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWS 300
           GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW+YVAPLKG FVVNLGDMLERWS
Sbjct: 268 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWQYVAPLKGTFVVNLGDMLERWS 327

Query: 301 NCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQR 360
           NCIFKSTLHRVLVNGQDRYSIAYFVEP+HDCLVECLPTC S+KNPPKFPPI C TYLSQR
Sbjct: 328 NCIFKSTLHRVLVNGQDRYSIAYFVEPNHDCLVECLPTCKSDKNPPKFPPIRCRTYLSQR 360

Query: 361 YKETHVDLNTY 371
           YKETHVDL+TY
Sbjct: 388 YKETHVDLSTY 360

BLAST of Spg003696 vs. NCBI nr
Match: KAG7030452.1 (encD [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620.2 bits (1598), Expect = 1.2e-173
Identity = 301/374 (80.48%), Postives = 317/374 (84.76%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T  DPNP F ALNCIDLSSSD+H SVSLLK                          
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGI EEFM EVFAQSKRLFSLPLSDKMKLLRNEKH
Sbjct: 61  ------------QACLDTGFFYAVNHGIPEEFMDEVFAQSKRLFSLPLSDKMKLLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTPILDELLDPDNQIRGDYKEGYYIG+EEKIPG+   GE PFYGPNIWPAEDLLPGW+
Sbjct: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDSGGEMPFYGPNIWPAEDLLPGWR 180

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           +IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEG+ISEPAKG
Sbjct: 181 KIMEKFHREALEVARAIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGKISEPAKG 240

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           IFGAGAHTDYGLITLLATDEN GLQICKDK+AKPQ+WEYVAPLKGAFVVNLGDMLERWSN
Sbjct: 241 IFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVWEYVAPLKGAFVVNLGDMLERWSN 300

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
           C+F+STLHRVL NGQDRYSIAYFVEPSHDCLVECLPTC S++NPPKFPPILC TYLSQRY
Sbjct: 301 CVFRSTLHRVLGNGQDRYSIAYFVEPSHDCLVECLPTCKSDENPPKFPPILCKTYLSQRY 336

Query: 361 KETHVDLNTYNNED 375
           KETHVDL+TY + +
Sbjct: 361 KETHVDLDTYKSSN 336

BLAST of Spg003696 vs. ExPASy Swiss-Prot
Match: C8VK14 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=hxnY PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.9e-44
Identity = 115/310 (37.10%), Postives = 166/310 (53.55%), Query Frame = 0

Query: 73  QACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLR--NEKHRGYTPILDEL 132
           ++C   GFF    H I  E    V   +KR F+LPL +KM + +  N  +RGY  +  ++
Sbjct: 31  ESCEYNGFFQITGHRIPRELQVRVMDAAKRFFALPLEEKMAIDKNLNSFNRGYELLRSQM 90

Query: 133 LDPDNQIRGDYKEGYYIGMEEKIPGEHP--DGERPFYGPNIWPAEDLLPGWKEIME---K 192
           L+       + KEG YIG  E+I  +HP     R   GPN WPA   +P  +E  E   +
Sbjct: 91  LEVGT--APELKEGLYIG--EEIGADHPYYINGRLNSGPNQWPA--TVPDAQEFRETSME 150

Query: 193 FHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQ-ISEPAKGIFGA 252
           ++    E+A+ +  ++AL L +E  FFD     G  +A +R+LHY  Q   E  K   G 
Sbjct: 151 YYHAVYELAKDVLAVLALTLDVEESFFDPLTEGG--VATMRMLHYPSQPKDEDEKLNRGI 210

Query: 253 GAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFK 312
           GAHTD+G ITLL  DE  GLQ+    DA    W  V P+ GA+VVNLGD++ R +N  +K
Sbjct: 211 GAHTDFGCITLLLQDEVDGLQVL---DAPSGQWLDVQPVLGAYVVNLGDLMMRMANDRYK 270

Query: 313 STLHRVL-VNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRYKET 372
           S +HRV+  +G++RYSI +F   + D + +CLP C      PK+PPI     +   YK++
Sbjct: 271 SNIHRVINKSGRERYSIPFFFSGNPDHVCKCLPNCCKAGEQPKYPPITVEDMVRGAYKQS 329

Query: 373 HVDLNTYNNE 374
           +     Y  E
Sbjct: 331 YGRAEAYKKE 329

BLAST of Spg003696 vs. ExPASy Swiss-Prot
Match: Q1ERI0 (2-oxoglutarate-dependent dioxygenase mpl2 OS=Monascus purpureus OX=5098 GN=mpl2 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.5e-37
Identity = 94/261 (36.02%), Postives = 142/261 (54.41%), Query Frame = 0

Query: 74  ACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDP 133
           AC  TGFF  + HGI+      VFA + + F+LP SD     RN   RGY P+  +  + 
Sbjct: 38  ACTSTGFFQLLGHGISPALQQSVFAAAAKFFALP-SDVKSRCRNVGFRGYDPMASQSYEL 97

Query: 134 DNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLP--GWKEIMEKFHRQAL 193
              +  D KEG+  G +  +       +R F G N WP  +LLP   ++  +E++++  L
Sbjct: 98  G--VLPDLKEGFIAGKDIPLDDPRVASQRFFMGQNAWPPSELLPEANFRRPIEEYYQAML 157

Query: 194 EVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHY-EGQISEPAKG-IFGAGAHTD 253
           ++  V+  L+A  L      FD+ +   +P   LRLLHY      + AKG   G+ AHTD
Sbjct: 158 KLCWVVLDLVAATLPYGPHVFDEFKE-NDPACPLRLLHYPPAPAPDVAKGRQLGSSAHTD 217

Query: 254 YGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHR 313
           +G ITLL  D++ GL++   +D +   W  V P K A+VVNLGDM+ R +   +KS++HR
Sbjct: 218 FGAITLLLQDDHSGLEV---QDCETGEWIGVPPNKDAYVVNLGDMMSRITRGHYKSSIHR 277

Query: 314 VL-VNGQDRYSIAYFVEPSHD 330
           V+  N  DRYS+ +F + + D
Sbjct: 278 VINQNLTDRYSVVFFFDGNLD 291

BLAST of Spg003696 vs. ExPASy Swiss-Prot
Match: A0A159BP93 (2-oxoglutarate-dependent dioxygenase citB OS=Monascus ruber OX=89489 GN=citB PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.5e-37
Identity = 94/261 (36.02%), Postives = 142/261 (54.41%), Query Frame = 0

Query: 74  ACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDP 133
           AC  TGFF  + HGI+      VFA + + F+LP SD     RN   RGY P+  +  + 
Sbjct: 38  ACTSTGFFQLLGHGISPALQQSVFAAAAKFFALP-SDVKSRCRNVGFRGYDPMASQSYEL 97

Query: 134 DNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLP--GWKEIMEKFHRQAL 193
              +  D KEG+  G +  +       +R F G N WP  +LLP   ++  +E++++  L
Sbjct: 98  G--VLPDLKEGFIAGKDIPLDDPRVASQRFFMGQNAWPPSELLPEANFRRPIEEYYQAML 157

Query: 194 EVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHY-EGQISEPAKG-IFGAGAHTD 253
           ++  V+  L+A  L      FD+ +   +P   LRLLHY      + AKG   G+ AHTD
Sbjct: 158 KLCWVVLDLVAATLPYGPHVFDEFKE-NDPACPLRLLHYPPAPAPDVAKGRQLGSSAHTD 217

Query: 254 YGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHR 313
           +G ITLL  D++ GL++   +D +   W  V P K A+VVNLGDM+ R +   +KS++HR
Sbjct: 218 FGAITLLLQDDHSGLEV---QDCETGEWIGVPPNKDAYVVNLGDMMSRITRGHYKSSIHR 277

Query: 314 VL-VNGQDRYSIAYFVEPSHD 330
           V+  N  DRYS+ +F + + D
Sbjct: 278 VINQNLTDRYSVVFFFDGNLD 291

BLAST of Spg003696 vs. ExPASy Swiss-Prot
Match: Q6K332 (2-oxoglutarate-dependent dioxygenase 33 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD33 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.6e-35
Identity = 99/295 (33.56%), Postives = 152/295 (51.53%), Query Frame = 0

Query: 74  ACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEK--HRGYTPILDELL 133
           AC + GFFY   HGI +  M +V   +++ F LP  +K+K+    +  +RGY  + + + 
Sbjct: 41  ACREAGFFYVKGHGIADSLMKQVRDVTQKFFQLPYEEKLKIKMTPQSGYRGYQRVGENIT 100

Query: 134 --DPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQ 193
              PD     D     Y  +E   PG++ D  +P  G N+WP       +  ++E +   
Sbjct: 101 KGKPDMHEAID----CYTPIE---PGKYGDLAKPMVGSNLWPKYP--SNFDVLLENYISL 160

Query: 194 ALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHY--EGQISEPAKGIFGAGAH 253
             +++R I R IALALG   D F+     G+P  V RL+ Y     I E  +   G GAH
Sbjct: 161 LRDLSRKIMRGIALALGAPVDAFE-GTTAGDPFWVCRLIGYPVSTDIPEEQRTDTGCGAH 220

Query: 254 TDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTL 313
           TDYGL+TL+  D+++     +++  +   W Y  P+ G FV N+GDML+ WSN I++ TL
Sbjct: 221 TDYGLLTLVNQDDDICALEVRNQSGE---WIYAKPIPGTFVCNIGDMLKVWSNGIYQPTL 280

Query: 314 HRVLVNGQDRY--SIAYFVEPSHDCLVECLPTCTSEK-NPPKFPPILCSTYLSQR 360
           HRV VN   RY  S+A+F E + D  VE +  C        K+  ++   +L Q+
Sbjct: 281 HRV-VNNSPRYRVSVAFFYESNFDAAVEPVEFCRERTGGVAKYEKVVYGEHLVQK 321

BLAST of Spg003696 vs. ExPASy Swiss-Prot
Match: A0A5C1RDG7 (2-oxoglutarate-dependent dioxygenase OS=Didymella fabae OX=372025 GN=orf3 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.7e-35
Identity = 87/265 (32.83%), Postives = 141/265 (53.21%), Query Frame = 0

Query: 73  QACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDK--MKLLRNEKHRGYTPILDEL 132
           +AC  TGFF    HG++++ + ++FA S + F+LP   K  +   +N   RGY  I  ++
Sbjct: 37  KACTSTGFFQLTGHGLSQDVVEDIFAASAKFFALPKDVKQGLNFTKNLGFRGYELIGAQV 96

Query: 133 LDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLP--GWKEIMEKFHR 192
            + D  +  D KEG+  G++          +R F G N+WP  D+LP   ++E +EK+++
Sbjct: 97  YESD--VLPDLKEGFLAGIDLPFEDMRVQNKRFFAGQNVWPPPDVLPYAEFREPVEKYYK 156

Query: 193 QALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKGI--FGAGA 252
             +++   +  L+A  L    D FD+ +   EP   LRLLHY    +  A      G+ A
Sbjct: 157 SIMQLCFTVMDLVAATLPYGPDVFDEWKS-HEPACPLRLLHYPPTPAHVAGKTRQLGSSA 216

Query: 253 HTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKST 312
           HTD+G +TLL  D + GL++          W  V P  GAFVVN+ DM+   +   +KS+
Sbjct: 217 HTDFGALTLLLQDSHEGLEVLNHDTGD---WVLVPPKPGAFVVNIADMMTMVTVGEYKSS 276

Query: 313 LHRVLVNG--QDRYSIAYFVEPSHD 330
            HRV+     +DRYS+ +F++ + D
Sbjct: 277 KHRVINRNETEDRYSVVFFMDGNVD 295

BLAST of Spg003696 vs. ExPASy TrEMBL
Match: A0A6J1G3R7 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.9e-173
Identity = 300/374 (80.21%), Postives = 316/374 (84.49%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T  DP+P F ALNCIDLSSSD+H SVSLLK                          
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGI EEFM EVFAQSKRLFSLPLSDKMKLLRNEKH
Sbjct: 61  ------------QACLDTGFFYAVNHGIPEEFMDEVFAQSKRLFSLPLSDKMKLLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTPILDELLDPDNQIRGDYKEGYYIG+EEKIPG+   GE PFYGPNIWPAEDLLPGW+
Sbjct: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDSGGEMPFYGPNIWPAEDLLPGWR 180

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           +IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG
Sbjct: 181 KIMEKFHREALEVARAIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           IFGAGAHTDYGLITLLATDEN GLQICKDK+AKPQ+WEYVAPLKGAFVVNLGDMLERWSN
Sbjct: 241 IFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVWEYVAPLKGAFVVNLGDMLERWSN 300

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
           C+F+STLHRVL NGQDRYSIAYFVEP HDCLVECLPTC S++NPPKFPPILC TYLSQRY
Sbjct: 301 CVFRSTLHRVLGNGQDRYSIAYFVEPRHDCLVECLPTCKSDENPPKFPPILCKTYLSQRY 336

Query: 361 KETHVDLNTYNNED 375
           KETHVDL+TY + +
Sbjct: 361 KETHVDLDTYKSSN 336

BLAST of Spg003696 vs. ExPASy TrEMBL
Match: A0A6J1G3P3 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 7.2e-172
Identity = 300/375 (80.00%), Postives = 316/375 (84.27%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T  DP+P F ALNCIDLSSSD+H SVSLLK                          
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGI EEFM EVFAQSKRLFSLPLSDKMKLLRNEKH
Sbjct: 61  ------------QACLDTGFFYAVNHGIPEEFMDEVFAQSKRLFSLPLSDKMKLLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGW 180
           RGYTPILDELLDPDNQIR GDYKEGYYIG+EEKIPG+   GE PFYGPNIWPAEDLLPGW
Sbjct: 121 RGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKIPGDDSGGEMPFYGPNIWPAEDLLPGW 180

Query: 181 KEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK 240
           ++IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK
Sbjct: 181 RKIMEKFHREALEVARAIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK 240

Query: 241 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWS 300
           GIFGAGAHTDYGLITLLATDEN GLQICKDK+AKPQ+WEYVAPLKGAFVVNLGDMLERWS
Sbjct: 241 GIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVWEYVAPLKGAFVVNLGDMLERWS 300

Query: 301 NCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQR 360
           NC+F+STLHRVL NGQDRYSIAYFVEP HDCLVECLPTC S++NPPKFPPILC TYLSQR
Sbjct: 301 NCVFRSTLHRVLGNGQDRYSIAYFVEPRHDCLVECLPTCKSDENPPKFPPILCKTYLSQR 337

Query: 361 YKETHVDLNTYNNED 375
           YKETHVDL+TY + +
Sbjct: 361 YKETHVDLDTYKSSN 337

BLAST of Spg003696 vs. ExPASy TrEMBL
Match: A0A6J1KDA3 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 9.4e-172
Identity = 299/372 (80.38%), Postives = 314/372 (84.41%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T  DPNP F ALNCIDLSSSD+H SVSLLK                          
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGI EEFM EVFAQSKRLFSLPL DKMKLLRNEKH
Sbjct: 61  ------------QACLDTGFFYAVNHGIPEEFMDEVFAQSKRLFSLPLCDKMKLLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTPILDELLDP+NQIRGDYKEGYYIG+EEK PG+   GE PFYGPNIWPAEDLLPGW+
Sbjct: 121 RGYTPILDELLDPENQIRGDYKEGYYIGIEEKNPGDDSGGEMPFYGPNIWPAEDLLPGWR 180

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           +IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG
Sbjct: 181 KIMEKFHREALEVARAIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           IFGAGAHTDYGLITLLATDEN GLQICKDKDAKP+IWEYVAPLKGAFVVNLGDMLERWSN
Sbjct: 241 IFGAGAHTDYGLITLLATDENFGLQICKDKDAKPRIWEYVAPLKGAFVVNLGDMLERWSN 300

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
           C+F+STLHRVL NGQDRYSIAYFVEPS+DCLVECLPTC S++NPPKFPPILC TYLSQRY
Sbjct: 301 CVFRSTLHRVLGNGQDRYSIAYFVEPSNDCLVECLPTCKSDENPPKFPPILCKTYLSQRY 334

Query: 361 KETHVDLNTYNN 373
           KETHVDL+TY +
Sbjct: 361 KETHVDLDTYKS 334

BLAST of Spg003696 vs. ExPASy TrEMBL
Match: A0A6J1KFK8 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.3e-170
Identity = 299/373 (80.16%), Postives = 314/373 (84.18%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T  DPNP F ALNCIDLSSSD+H SVSLLK                          
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFYAVNHGI EEFM EVFAQSKRLFSLPL DKMKLLRNEKH
Sbjct: 61  ------------QACLDTGFFYAVNHGIPEEFMDEVFAQSKRLFSLPLCDKMKLLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGW 180
           RGYTPILDELLDP+NQIR GDYKEGYYIG+EEK PG+   GE PFYGPNIWPAEDLLPGW
Sbjct: 121 RGYTPILDELLDPENQIRVGDYKEGYYIGIEEKNPGDDSGGEMPFYGPNIWPAEDLLPGW 180

Query: 181 KEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK 240
           ++IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK
Sbjct: 181 RKIMEKFHREALEVARAIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK 240

Query: 241 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWS 300
           GIFGAGAHTDYGLITLLATDEN GLQICKDKDAKP+IWEYVAPLKGAFVVNLGDMLERWS
Sbjct: 241 GIFGAGAHTDYGLITLLATDENFGLQICKDKDAKPRIWEYVAPLKGAFVVNLGDMLERWS 300

Query: 301 NCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQR 360
           NC+F+STLHRVL NGQDRYSIAYFVEPS+DCLVECLPTC S++NPPKFPPILC TYLSQR
Sbjct: 301 NCVFRSTLHRVLGNGQDRYSIAYFVEPSNDCLVECLPTCKSDENPPKFPPILCKTYLSQR 335

Query: 361 YKETHVDLNTYNN 373
           YKETHVDL+TY +
Sbjct: 361 YKETHVDLDTYKS 335

BLAST of Spg003696 vs. ExPASy TrEMBL
Match: A0A0A0LIR5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000940 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 5.1e-170
Identity = 293/370 (79.19%), Postives = 311/370 (84.05%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MENPT+ DPNP+FLALNCIDLSSSDV+ SVSLLK                          
Sbjct: 1   MENPTENDPNPEFLALNCIDLSSSDVNLSVSLLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLDTGFFY VNHGITEEFM EVFAQSKRLF LPLSDKMKLLRNE H
Sbjct: 61  ------------QACLDTGFFYVVNHGITEEFMDEVFAQSKRLFDLPLSDKMKLLRNENH 120

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTPILDELLDP+NQIRGDYKEGYYIG+EE  PG+  DG+RPFYGPN+WPAED+LP W 
Sbjct: 121 RGYTPILDELLDPENQIRGDYKEGYYIGIEETNPGDGSDGKRPFYGPNVWPAEDMLPDWS 180

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           +IMEKFH QALEVARV+ARLIALALGLE +FFDKSEMLG+P A LRLLHYEGQISEPAKG
Sbjct: 181 KIMEKFHAQALEVARVVARLIALALGLEKEFFDKSEMLGKPTATLRLLHYEGQISEPAKG 240

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQ+W+YVAPLKGAFVVNLGDMLERWSN
Sbjct: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQVWQYVAPLKGAFVVNLGDMLERWSN 300

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
           CIFKSTLHRVLV+GQDRYSIAYFVEP+ DCLVECLPTC S+KNPPKFPPI C TYLSQRY
Sbjct: 301 CIFKSTLHRVLVSGQDRYSIAYFVEPNQDCLVECLPTCKSDKNPPKFPPIRCGTYLSQRY 332

Query: 361 KETHVDLNTY 371
           KETHVDL TY
Sbjct: 361 KETHVDLETY 332

BLAST of Spg003696 vs. TAIR 10
Match: AT1G35190.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 466.1 bits (1198), Expect = 2.7e-131
Identity = 223/373 (59.79%), Postives = 278/373 (74.53%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T +    K  +LNCIDL++ D++ SV  LK                          
Sbjct: 1   MENHTTM----KVSSLNCIDLANDDLNHSVVSLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLD GFFY +NHGI+EEFM +VF QSK+LF+LPL +KMK+LRNEKH
Sbjct: 61  ------------QACLDCGFFYVINHGISEEFMDDVFEQSKKLFALPLEEKMKVLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTP+LDELLDP NQI GD+KEGYYIG+E  +P + P  ++PFYGPN WP  D+LPGW+
Sbjct: 121 RGYTPVLDELLDPKNQINGDHKEGYYIGIE--VPKDDPHWDKPFYGPNPWPDADVLPGWR 180

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           E MEK+H++AL V+  IARL+ALAL L+  +FD++EMLG+PIA +RLL Y+G IS+P+KG
Sbjct: 181 ETMEKYHQEALRVSMAIARLLALALDLDVGYFDRTEMLGKPIATMRLLRYQG-ISDPSKG 240

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           I+  GAH+D+G++TLLATD  +GLQICKDK+A PQ WEYV P+KGAF+VNLGDMLERWSN
Sbjct: 241 IYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWEYVPPIKGAFIVNLGDMLERWSN 300

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
             FKSTLHRVL NGQ+RYSI +FVEP+HDCLVECLPTC SE   PK+PPI CSTYL+QRY
Sbjct: 301 GFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESELPKYPPIKCSTYLTQRY 328

Query: 361 KETHVDLNTYNNE 374
           +ETH +L+ Y+ +
Sbjct: 361 EETHANLSIYHQQ 328

BLAST of Spg003696 vs. TAIR 10
Match: AT1G35190.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.7e-120
Identity = 211/373 (56.57%), Postives = 261/373 (69.97%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEVE 60
           MEN T +    K  +LNCIDL++ D++ SV  LK                          
Sbjct: 1   MENHTTM----KVSSLNCIDLANDDLNHSVVSLK-------------------------- 60

Query: 61  LLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKH 120
                       QACLD GFFY +NHGI+EEFM +VF QSK+LF+LPL +KMK+LRNEKH
Sbjct: 61  ------------QACLDCGFFYVINHGISEEFMDDVFEQSKKLFALPLEEKMKVLRNEKH 120

Query: 121 RGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWK 180
           RGYTP+LDELLDP NQI GD+KEGYYIG+E  +P + P  ++PFYGPN WP  D+LPGW+
Sbjct: 121 RGYTPVLDELLDPKNQINGDHKEGYYIGIE--VPKDDPHWDKPFYGPNPWPDADVLPGWR 180

Query: 181 EIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKG 240
           E MEK+H++AL                       +EMLG+PIA +RLL Y+G IS+P+KG
Sbjct: 181 ETMEKYHQEAL-----------------------TEMLGKPIATMRLLRYQG-ISDPSKG 240

Query: 241 IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSN 300
           I+  GAH+D+G++TLLATD  +GLQICKDK+A PQ WEYV P+KGAF+VNLGDMLERWSN
Sbjct: 241 IYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWEYVPPIKGAFIVNLGDMLERWSN 300

Query: 301 CIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQRY 360
             FKSTLHRVL NGQ+RYSI +FVEP+HDCLVECLPTC SE   PK+PPI CSTYL+QRY
Sbjct: 301 GFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESELPKYPPIKCSTYLTQRY 305

Query: 361 KETHVDLNTYNNE 374
           +ETH +L+ Y+ +
Sbjct: 361 EETHANLSIYHQQ 305

BLAST of Spg003696 vs. TAIR 10
Match: AT3G46490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 424.9 bits (1091), Expect = 7.0e-119
Identity = 204/368 (55.43%), Postives = 258/368 (70.11%), Query Frame = 0

Query: 1   MENPTKIDPN-PKFLALNCIDLSSSDVHQSVSLLKQVICNMMIVLVDFQFRFVFCVEIEV 60
           MEN T+ + +  K  +L CIDL +SD+HQS  LLK                         
Sbjct: 1   MENKTEEEVSIIKVSSLTCIDLDNSDLHQSAVLLK------------------------- 60

Query: 61  ELLFGDWRSFLFFQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEK 120
                        QACLD+GFFY +NHGI+EE   E F  SK+ F+LPL +KMK+LRNEK
Sbjct: 61  -------------QACLDSGFFYVINHGISEELKDEAFEHSKKFFALPLEEKMKVLRNEK 120

Query: 121 HRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGW 180
           +RGY P  D LLDP+NQ+RGDYKEG+ IG E    G H D  +PF+ PNIWP  D+LPGW
Sbjct: 121 YRGYAPFHDSLLDPENQVRGDYKEGFTIGFEGSKDGPHWD--KPFHSPNIWPNPDVLPGW 180

Query: 181 KEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAK 240
           +E MEK++++AL V + IA+++ALAL L+ D+F+  EMLG PIA + L HYEG+ S+P+K
Sbjct: 181 RETMEKYYQEALRVCKSIAKIMALALDLDVDYFNTPEMLGNPIADMVLFHYEGK-SDPSK 240

Query: 241 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWS 300
           GI+  GAH+D+G+++LLATD  +GLQICKDKD KPQ WEY   +KGA++VNLGD+LERWS
Sbjct: 241 GIYACGAHSDFGMMSLLATDGVMGLQICKDKDVKPQKWEYTPSIKGAYIVNLGDLLERWS 300

Query: 301 NCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPILCSTYLSQR 360
           N  FKSTLHRVL NGQDRYSI +F++PSHDC++ECLPTC SE N PK+P I CSTY+SQR
Sbjct: 301 NGYFKSTLHRVLGNGQDRYSIPFFLKPSHDCIIECLPTCQSENNLPKYPAIKCSTYISQR 327

Query: 361 YKETHVDL 368
           YK  H  L
Sbjct: 361 YKAVHAHL 327

BLAST of Spg003696 vs. TAIR 10
Match: AT3G46480.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 372.9 bits (956), Expect = 3.1e-103
Identity = 171/272 (62.87%), Postives = 215/272 (79.04%), Query Frame = 0

Query: 99  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 158
           QSK+ F+LPL +KMK+LRNEKHRGY+P+LD++LDP+NQ+ GDYKE ++IG+E        
Sbjct: 36  QSKKFFALPLEEKMKVLRNEKHRGYSPVLDQILDPENQVDGDYKESFFIGIEV------- 95

Query: 159 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 218
                           +LPGW+  MEK+H++AL V + IARL+ALAL L+ ++FDK EML
Sbjct: 96  ----------------VLPGWRATMEKYHQEALRVCKAIARLLALALDLDTNYFDKPEML 155

Query: 219 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 278
           G PIAV+RLL YEG +S+P KGIFG GAH+DYG++TLLATD   GLQICKDKD KP+ WE
Sbjct: 156 GNPIAVMRLLRYEG-MSDPLKGIFGCGAHSDYGMLTLLATDSVTGLQICKDKDVKPRKWE 215

Query: 279 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTC 338
           YV  +KGA++VNLGD+LERWSN IFKSTLHRVL NGQDRYSI +F+EPSHDCLVECLPTC
Sbjct: 216 YVPSIKGAYIVNLGDLLERWSNGIFKSTLHRVLGNGQDRYSIPFFIEPSHDCLVECLPTC 275

Query: 339 TSEKNPPKFPPILCSTYLSQRYKETHVDLNTY 371
            SE N PK+P I CST+L+QRY+++HV+L+ Y
Sbjct: 276 QSENNLPKYPAIKCSTFLTQRYQQSHVNLSIY 283

BLAST of Spg003696 vs. TAIR 10
Match: AT3G46500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 4.4e-97
Identity = 155/259 (59.85%), Postives = 206/259 (79.54%), Query Frame = 0

Query: 112 MKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWP 171
           MK+L+NEKH+GY+P+L ++   DNQI GDYKE ++IG+E          + PF   NIWP
Sbjct: 1   MKVLKNEKHQGYSPVLSQI--SDNQIHGDYKESFFIGIE-------GSNDTPFCRANIWP 60

Query: 172 AEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYE 231
             D+L GW+  MEK+H++AL V + IAR++ALAL ++ D+FD  EMLG P+  +RLLHYE
Sbjct: 61  NPDVLSGWQATMEKYHQEALRVCKAIARVLALALNVDGDYFDTPEMLGNPLTFMRLLHYE 120

Query: 232 GQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNL 291
           G +S+P+KGI+G G H+D+G++TLL TD  +GLQICKD+D KP+ WEY+  +KGA++VN+
Sbjct: 121 G-MSDPSKGIYGCGPHSDFGMMTLLGTDSVMGLQICKDRDVKPRKWEYILSIKGAYIVNI 180

Query: 292 GDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCTSEKNPPKFPPIL 351
           GD+LERWSN IFKSTLHRVL NGQDRYSIA+F++PSHDC+VECLPTC SE NPPK+P I 
Sbjct: 181 GDLLERWSNGIFKSTLHRVLGNGQDRYSIAFFLQPSHDCIVECLPTCQSENNPPKYPAIK 240

Query: 352 CSTYLSQRYKETHVDLNTY 371
           CSTYL+QRY+++ VDL+ Y
Sbjct: 241 CSTYLTQRYQDSQVDLSIY 249