Spg003579 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003579
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pseudouridine synthase family protein
Location	scaffold4: 44005853 .. 44008844 (+)
RNA-Seq Expression	Spg003579
Synteny	Spg003579

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAAACTCGAGTTCAAGGATTCTATTTGGAACAATACTTGGTTACATATCAGTCCTATCCCTACCTATCAGTGTGTAGCTAAGTATTTTTCTTCCCTTTGCCAAAAAGAAAAACTTGAAATTAAAAAATAAAAATAAAAAAAAATTAAAGACTTTTATATAATTCAGTTTTAGGACCAAAAAAGCTTTAAATAACAAAAATGGCCCTCTTCGTCGCACTTCATCCCACTTTCTCCATTCCCCACAAAACCTTCACCAAATTCCCAAAACCCTTTTGTTTCCATAGATTCAAACCAGTCTGCAATAGCATGGCGGACCCATCAGAACCGGCGACCAGAAACTGCCCAACCGCCGGAGACAATTACCCGGTGCCCCTATCGCCTCCATTGCAGGCCATATCCAAGAACTTGGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAGCGATGTCATATACGAGGATGAATGGCTCATTGCCGTCAATAAGCCTCAAGGGGTCTACTGTGAGAGCGTTTTGGCCTCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGTGACCGGAGTGCAGGTACTAGAGCTCTCTCTGCCTATGTGGGTTATTTGCTTAGCTTGTGAGTTTGCCATTAAGAGCAACTGGGTTGTGTTGGATTTTGGTATGAGAATTTTTTTTTAGACAAATTCAGAATTTAACATGAACTTGGGAAGGTGAATTCAATTTTACCCTAAATTTTGTATTTGATAAGCGAGTGGGAATGGTGCAGGTGCAACTTTTAACTTGCTTTCATTTGAACAAGTGTAAAGAGGTATTATCGTGTGCTTTGCTTTCAATGGATTAGAAAATATCGACGAAGCTGCAAAGAAAGAAGTTATCAGGGCTGATTTTTTTTTTTTTTTTTGGGTTAAAGTTGATGAGTTTTGAAAGATTTATTCCATATAGATCTCGTGCATTGATTTTCTGAATCATACTTTTGTGCATTGATCTGTAACTTGGTTTCTCTTGAAATTAGCTGAAATTAGTGGGTAAATGTAATGGAAGTCTATCAAAAGTTTAAAATTGTAATACTTATCTATTAGTAAGGTCCAATTTGACGAAATTTAAAGTTTGGAGCAACAATTGACTCACCTTGCTAAGGCTCCATTTGGGATTGATCTTGCACGTGATAAAAGTGATTACAGGGGAATTCCTTTTTTTTTTTTTTTAATTTTATGTCTAGTAAAACTGATTATTAAATAATCTTAAGCAAATCACTTTTAAGCTCTTCCCTTTTTTGTTTTTCATGAAACTCCTCGTCTATTGGTTTTTCCAAGAGAGACACAAAAATCCATCTCAAGTCTTTTTAAAACAATCATCTCAACAAGAGTTTATAAAGAACCTTTTAGGTTATCTCATCAATTTAGATGGTATTGTAGTTTTCTGGGGTGCTCTATGATATCATGATAGGTGGGGATGCTTGGGGAGTTTGATCATTAGACAGTTGATATAAAAATGGTTTAGCTGTATCCTGGTTGTTGAAAGCATTTGACAGTCCATGCTTGAATGTGTCTGAAGTGTTAAGCTTTTGTCTAAAACGATATGATTATTCTATTACTATTGATTTATTGGGTTGTTCTCTGCTTGGATTCTTCATATGCAGAGATCAAGATCAGTGTGCCAGAGCTTCATCTCGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTGTGTGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCAAAGTTCGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAGACCTATTTTGAAGTGTTGTCTGTAAATGGACAAAGCACCATGGAGGAACTTCCAGAATCTAGGAAAGATGAAGAAGAAACTATTGTAGTCCAAACAAAATCTCTGATAGATATTGATGCTCAGAAGGATGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAGTATGAGGGTGTTACAGAGTGGAACGGAAAAATTTGTGATGGCCATGAGCTTCATGCTGAAAGCTTGTATTTAGCACACCCTGTTACAGGTATTCCTCTCAAACTGCAGGCCCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAACAAGTGAATTCTCCATCATCCTTGAAACCTATGCCTTAACAAGATCATTGGCACGGTTAAGTGAGAACCAAATATATCTCTTATTATACGAAGAGCCTTGGTGTTGAAGAATGTCGATAAAGCAAAATGGGACAATGTTCAATCAAATTCGTACAAGTGTGACTACAGACAGTTTTCGCATCATTGGCTTCTTGTCTGCATTCTCTCATCTACACTTGATATGTCTGCTCCTTATTGAAGCAGAGATTTGAATGAGCTCTTGCTAGCACAACAGTCTCTATGAAAATATCAACGAAGTCATAAACCGACCAACTTGCAGAATTTCGCCTGAAAATTTGAGAGAATGGGATGCTCACAACAGGTTCTGGAAATGAAAGAAGAAGCATGCATGTCAAATGCTGATAAGTTTGTGCAAAGCCCTTTGAAAATGCATCTTCTGCAGCCATTTATTGATAGAATTGATGTACAATGCTGAAGAAAAATCTATGGTTAGCATGTTATTGGTTTATGTTGATGAGGTTCAATTGTTTTTTCTGTCTCACTCCCTCGTTTGTGCAGCTTAATGATCTAAATATGACAAACAATTTGTAATGATGTCCATGCGGCTATAAACTATAAATAGAAGAATGAGAGCGTGTTGAGTATTTGATTTTTGTTG

mRNA sequence

ATGGCCCTCTTCGTCGCACTTCATCCCACTTTCTCCATTCCCCACAAAACCTTCACCAAATTCCCAAAACCCTTTTGTTTCCATAGATTCAAACCAGTCTGCAATAGCATGGCGGACCCATCAGAACCGGCGACCAGAAACTGCCCAACCGCCGGAGACAATTACCCGGTGCCCCTATCGCCTCCATTGCAGGCCATATCCAAGAACTTGGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAGCGATGTCATATACGAGGATGAATGGCTCATTGCCGTCAATAAGCCTCAAGGGGTCTACTGTGAGAGCGTTTTGGCCTCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGTGACCGGAGTGCAGAGATCAAGATCAGTGTGCCAGAGCTTCATCTCGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTGTGTGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCAAAGTTCGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAGACCTATTTTGAAGTGTTGTCTGTAAATGGACAAAGCACCATGGAGGAACTTCCAGAATCTAGGAAAGATGAAGAAGAAACTATTGTAGTCCAAACAAAATCTCTGATAGATATTGATGCTCAGAAGGATGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAGTATGAGGGTGTTACAGAGTGGAACGGAAAAATTTGTGATGGCCATGAGCTTCATGCTGAAAGCTTGTATTTAGCACACCCTGTTACAGGTATTCCTCTCAAACTGCAGGCCCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAACAAGTGAATTCTCCATCATCCTTGAAACCTATGCCTTAA

Coding sequence (CDS)

ATGGCCCTCTTCGTCGCACTTCATCCCACTTTCTCCATTCCCCACAAAACCTTCACCAAATTCCCAAAACCCTTTTGTTTCCATAGATTCAAACCAGTCTGCAATAGCATGGCGGACCCATCAGAACCGGCGACCAGAAACTGCCCAACCGCCGGAGACAATTACCCGGTGCCCCTATCGCCTCCATTGCAGGCCATATCCAAGAACTTGGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAGCGATGTCATATACGAGGATGAATGGCTCATTGCCGTCAATAAGCCTCAAGGGGTCTACTGTGAGAGCGTTTTGGCCTCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGTGACCGGAGTGCAGAGATCAAGATCAGTGTGCCAGAGCTTCATCTCGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTGTGTGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCAAAGTTCGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAGACCTATTTTGAAGTGTTGTCTGTAAATGGACAAAGCACCATGGAGGAACTTCCAGAATCTAGGAAAGATGAAGAAGAAACTATTGTAGTCCAAACAAAATCTCTGATAGATATTGATGCTCAGAAGGATGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAGTATGAGGGTGTTACAGAGTGGAACGGAAAAATTTGTGATGGCCATGAGCTTCATGCTGAAAGCTTGTATTTAGCACACCCTGTTACAGGTATTCCTCTCAAACTGCAGGCCCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAACAAGTGAATTCTCCATCATCCTTGAAACCTATGCCTTAA

Protein sequence

MALFVALHPTFSIPHKTFTKFPKPFCFHRFKPVCNSMADPSEPATRNCPTAGDNYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWASQALQPQQQVNSPSSLKPMP

Homology

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BLAST of Spg003579 vs. NCBI nr
Match: KAG7021056.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620.2 bits (1598), Expect = 1.2e-173
Identity = 322/373 (86.33%), Postives = 335/373 (89.81%), Query Frame = 0


Query: 1   MALFVALHPTFSIPHKTFTKFPKPFCFHRFKPVCNSMADPSEPATRNCPTAGDNYPVPLS 60
           MAL V + P FSIPHK+F KFPKPF FHRF+PV NSMAD S+P T      G+NYPVPLS
Sbjct: 6   MALVVTIQPPFSIPHKSFAKFPKPFRFHRFRPVTNSMADSSKPVT------GENYPVPLS 65

Query: 61  PPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESVLASVPRL 120
           PPL AISKNLELARAMAA SKSSLF+LSA DVIYEDEWLIAVNKPQGVYCESVLASVPR 
Sbjct: 66  PPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQGVYCESVLASVPRF 125

Query: 121 LGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY 180
           LGDSAMAG +S EIKIS+PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY
Sbjct: 126 LGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY 185

Query: 181 IALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNG 240
           IA CVGSAPKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSVNG
Sbjct: 186 IAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNG 245

Query: 241 QSTMEELPESRKD----EEETIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYL 300
           Q+T EELPESRKD    EEETIV Q+KSLIDIDA+KDEILIRARPRSGRTHQIRLH QYL
Sbjct: 246 QNTKEELPESRKDEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYL 305

Query: 301 GIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWASQALQPQQ 360
           GIPI GDVKYEGVTEWNGKI D HELHAESLY  HPVTGIPLKLQAPLPSWA+QALQPQQ
Sbjct: 306 GIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQPQQ 365

Query: 361 Q-VNSPSSLKPMP 369
           Q VNS +SLKPMP
Sbjct: 366 QEVNSAASLKPMP 372

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BLAST of Spg003579 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 614.0 bits (1582), Expect = 8.5e-172
Identity = 312/371 (84.10%), Postives = 332/371 (89.49%), Query Frame = 0


Query: 1   MALFVALHPTFSIPHKTFTKFP--KPFCFHRFKPVCNSMADPSEPATRNCPTAGDNYPVP 60
           MALF++LHP FSI  KTFT+FP  K FCFHRFK V NSM D SEP T NCP+  +NYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESVLASVP 120
           LSPPL AISKNLELARAM ASSKSSL+ALSA+DVIYEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180
           RLLGDSA AG     IK S+PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 121 RLLGDSANAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180

Query: 181 SYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240
           SYIA CVG++PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV
Sbjct: 181 SYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240

Query: 241 NGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGI 300
           NG++TMEEL + R+ EEETIVV TKSL+DID+ KDEILIRARPRSGRTHQIRLHCQYLGI
Sbjct: 241 NGKNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGI 300

Query: 301 PIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWASQALQPQQ-Q 360
           PIRGDVKYEGVTEWN KI D HELHAESLY  HPVTGIPLKLQAPLPSWASQALQPQQ +
Sbjct: 301 PIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHE 360

Query: 361 VNSPSSLKPMP 369
           VNSP S K MP
Sbjct: 361 VNSPQSFKTMP 366

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BLAST of Spg003579 vs. NCBI nr
Match: KAG6586224.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.5 bits (1578), Expect = 2.5e-171
Identity = 319/375 (85.07%), Postives = 334/375 (89.07%), Query Frame = 0


Query: 1   MALFVALHPTFSIPHKTFTKFPKPFCFHRFKPVCNSMADPSEPATRNCPTAGDNYPVPLS 60
           MAL V L   FSIP+K+F KFPKPF FHRF+PV NSMAD S+P T      G+NYPVPLS
Sbjct: 1   MALVVTLQAPFSIPNKSFAKFPKPFRFHRFRPVTNSMADSSKPVT------GENYPVPLS 60

Query: 61  PPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESVLASVPRL 120
           PPL AISKNLELARAMAA SKSSLF+LSA DV+YEDEWLIAVNKPQGVYCESVLASVP  
Sbjct: 61  PPLPAISKNLELARAMAACSKSSLFSLSACDVVYEDEWLIAVNKPQGVYCESVLASVPCF 120

Query: 121 LGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY 180
           LGDSAMAG++S EIKIS+PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY
Sbjct: 121 LGDSAMAGEQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY 180

Query: 181 IALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNG 240
           IA CVGSAPKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSVNG
Sbjct: 181 IAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNG 240

Query: 241 QSTMEELPESRKD------EEETIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQ 300
           Q+T EELPESRKD      EEETIV Q+KSLIDIDA+KDEILIRARPRSGRTHQIRLH Q
Sbjct: 241 QNTKEELPESRKDEEEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQ 300

Query: 301 YLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWASQALQP 360
           YLGIPI GDVKYEGVTEWNGKI D HELHAESLY  HPVTGIPLKLQAPLPSWA+QALQP
Sbjct: 301 YLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQP 360

Query: 361 QQQ-VNSPSSLKPMP 369
           QQQ VNS +SLKPMP
Sbjct: 361 QQQEVNSAASLKPMP 369

[Close]

BLAST of Spg003579 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 610.5 bits (1573), Expect = 9.3e-171
Identity = 309/362 (85.36%), Postives = 326/362 (90.06%), Query Frame = 0


Query: 1   MALFVALHPTFSIPHKTFTKFPKPFCFHRFKPVCNSMADPSEPATRNCPTAGDNYPVPLS 60
           MAL ++L PTFS PHKTFT+FPKPFC  RFK +  SMAD SEP T NCP+  +NYPVPLS
Sbjct: 1   MALLISLQPTFSTPHKTFTEFPKPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVPLS 60

Query: 61  PPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESVLASVPRL 120
           PPL AISKNLELARAMAASSKSSLFALSA+DVIYEDEWLIAVNKPQG+YCESVLA+VPRL
Sbjct: 61  PPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVPRL 120

Query: 121 LGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY 180
           L DSAMAG     IK S+PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSKSY
Sbjct: 121 LSDSAMAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSY 180

Query: 181 IALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNG 240
           IA CVGSAPKWKKIN+KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLS+NG
Sbjct: 181 IAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSING 240

Query: 241 QSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGIPI 300
           Q+ MEE  +  KD EET+VVQTKSL+DID QKDEILIRARPRSGRTHQIRLHCQYLGIPI
Sbjct: 241 QNAMEEFQKFGKD-EETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPI 300

Query: 301 RGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWASQALQPQQQ-VN 360
           RGDVKYEGVTEWNGKI D HELHAESLY  HP+TGIPLKLQAPLPSWASQA QPQQQ VN
Sbjct: 301 RGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVN 356

Query: 361 SP 362
           SP
Sbjct: 361 SP 356

[Close]

BLAST of Spg003579 vs. NCBI nr
Match: XP_022156345.1 (RNA pseudouridine synthase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 580.5 bits (1495), Expect = 1.0e-161
Identity = 295/330 (89.39%), Postives = 307/330 (93.03%), Query Frame = 0


Query: 37  MADPSEPATRNCPTAGDNYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYED 96
           MADPS P T N P  G NYP+PLSPPL AISKNLELARAMAASSKSSLFALSA+DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 97  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMV 156
           EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG++S  I+IS+PELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 157 ITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 216
           ITKSHKVASKLVKAFTDH VSKSYIA CVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 217 GRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEIL 276
           GRSLPGGSVVRDMETYFEVLSVNGQSTM+ELPE RKDEEETIVVQ+KSLIDIDA KDEIL
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 277 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGI 336
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG I D HELHAESL+  HPVTGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 337 PLKLQAPLPSWASQALQPQQQ-VNSPSSLK 366
           PLKLQAPLPSWASQAL  QQQ V+SPS+LK
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLK 330

[Close]

BLAST of Spg003579 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 3.8e-98
Identity = 185/298 (62.08%), Postives = 223/298 (74.83%), Query Frame = 0


Query: 54  NYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESV 113
           NYP P+S P   ISK++EL RAM ASSKSSLF L+  D++YEDE+L+AVNKP+GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 114 LASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTD 173
           L S P+++ DS+              E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+
Sbjct: 99  LRSAPQIVLDSS-------------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTE 158

Query: 174 HKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYF 233
           HK+ KSYIALC+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET F
Sbjct: 159 HKIRKSYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTF 218

Query: 234 EVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDE--ILIRARPRSGRTHQIRL 293
           EV+SVN  S   E  E  +D    IV + +  +      D+  +++RA PRSGRTHQIRL
Sbjct: 219 EVVSVN--SVKNESCE-LEDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRL 278

Query: 294 HCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWAS 350
           HCQYLGIPIRGDVKY GV EWNG+  +GHELHAE L L HPVTG  + ++APLP WA+
Sbjct: 279 HCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of Spg003579 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.4e-97
Identity = 182/304 (59.87%), Postives = 225/304 (74.01%), Query Frame = 0


Query: 51  AGDNYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYC 110
           A   YPVP+SPP  A SK++EL RAM AS++S+  A S++ V++EDEWL  V+KP GVYC
Sbjct: 47  ATGEYPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVYC 106

Query: 111 ESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKA 170
           +++L+++P     +A  GD + +     P LHLANRLDRDTSG+MVITK +KVA KLVKA
Sbjct: 107 DALLSALP---CSAATLGDEATK-----PNLHLANRLDRDTSGLMVITKCNKVAGKLVKA 166

Query: 171 FTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDME 230
           FT+HKV K+Y+ALC+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGSVVRDM 
Sbjct: 167 FTEHKVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMS 226

Query: 231 TYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDE----ILIRARPRSGRT 290
           T FEVL +NG+    E      DE E+I VQ K+  D+ +  DE    IL+RA P+SGRT
Sbjct: 227 TRFEVLGINGKGQFREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRT 286

Query: 291 HQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPS 350
           HQIRLHCQYLG PIRGDVKY GV EWNG   DGH LHAESL   HPVTG+P+  ++PLPS
Sbjct: 287 HQIRLHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPS 339

[Close]

BLAST of Spg003579 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.6e-17
Identity = 82/264 (31.06%), Postives = 124/264 (46.97%), Query Frame = 0


Query: 92  VIYEDEWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDT 151
           +IYED+ LIA+NKP  +  +    S   L  DSA+       +     +  L +RLD++T
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSAL-----KYLNYQGADFKLVHRLDKET 150

Query: 152 SGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVY 211
           SG+++I K++  + KL  AF +  V K Y A+  G   K   I VKS  G+SK    R++
Sbjct: 151 SGLLLIAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSNIGKSK---GRMF 210

Query: 212 AAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQ 271
              D+      G +     TY+++L                          KSL      
Sbjct: 211 KITDIDSD--NGKLA---ITYYKLL--------------------------KSL-----N 270

Query: 272 KDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHE---LHAESLY 331
            +  LI   P +GR HQ+RLH Q LG PI GD KY      N ++    +   LHA ++Y
Sbjct: 271 NNLFLIEFMPVTGRMHQLRLHAQLLGCPILGDDKY-----GNKEVMPYSKYMFLHANNIY 302

Query: 332 LAHPVTGIPLKLQAPLPSWASQAL 353
           L+  + G  +KL+A LP + ++ L
Sbjct: 331 LSESIVGKEIKLEAKLPFYFTRRL 302

[Close]

BLAST of Spg003579 vs. ExPASy Swiss-Prot
Match: Q8PHN2 (Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. citri (strain 306) OX=190486 GN=rluD PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.1e-16
Identity = 72/266 (27.07%), Postives = 116/266 (43.61%), Query Frame = 0


Query: 92  VIYEDEWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDT 151
           V+YEDE ++ ++KP G+       +  R L ++ +  D +     SVP   + +RLD+DT
Sbjct: 92  VLYEDEQVLVIDKPAGLVVHPGAGNPDRTLVNALLFRDPAL---ASVPRAGVVHRLDKDT 151

Query: 152 SGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVK-SGHGRSKFGVWRV 211
           SGVMV+ ++ +  + LV+  +  +V + Y+A+ VG+       N     H R +  +   
Sbjct: 152 SGVMVVARTVQAQTALVEQLSAREVHRQYLAVVVGALVSGGTANAPIDRHPRDRLKM--- 211

Query: 212 YAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDA 271
            A  D G         RD  T++ +                                 + 
Sbjct: 212 -AVRDDG---------RDAVTHYRLR--------------------------------ER 271

Query: 272 QKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDG--HE------- 331
            +    +  R  +GRTHQIR+H  +L  PI GD  Y G  +      D   HE       
Sbjct: 272 FRAHTALECRLETGRTHQIRVHMAHLKSPIVGDPLYGGALKLPKGATDELVHELRTFKRQ 309

Query: 332 -LHAESLYLAHPVTGIPLKLQAPLPS 347
            LHAE+L   HPV+G P++  AP+P+
Sbjct: 332 ALHAETLEFLHPVSGEPVRASAPVPA 309

[Close]

BLAST of Spg003579 vs. ExPASy Swiss-Prot
Match: Q9ZDR7 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.8e-16
Identity = 82/264 (31.06%), Postives = 124/264 (46.97%), Query Frame = 0


Query: 92  VIYEDEWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDT 151
           +IYED+ LIA+NKP  +  +    S   L  DSA+       +     +  L +RLD++T
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSAL-----KYLNYQGADFKLVHRLDKET 150

Query: 152 SGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVY 211
           SG+++I K++  + KL  AF +  V K Y A+  G  P      VKS  G+SK    R+ 
Sbjct: 151 SGLLLIAKNYLSSVKLHNAFKEKLVIKKYFAITYG-RPVKNVGTVKSNIGKSK---RRLS 210

Query: 212 AAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQ 271
             A++  S  G   +    TY+++L                          KSL      
Sbjct: 211 KIANID-SDDGKLAI----TYYKLL--------------------------KSL-----N 270

Query: 272 KDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHE---LHAESLY 331
            +  LI   P +GR HQ+RLH Q LG PI GD KY      N +I    +   LHA ++Y
Sbjct: 271 NNLFLIEFMPVTGRMHQLRLHAQLLGCPILGDDKY-----GNKEIMPYSKYMFLHANNIY 302

Query: 332 LAHPVTGIPLKLQAPLPSWASQAL 353
           L+  + G  +KL+A LP + ++ +
Sbjct: 331 LSETIVGKEIKLEAKLPFYFTRRI 302

[Close]

BLAST of Spg003579 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.1e-172
Identity = 312/371 (84.10%), Postives = 332/371 (89.49%), Query Frame = 0


Query: 1   MALFVALHPTFSIPHKTFTKFP--KPFCFHRFKPVCNSMADPSEPATRNCPTAGDNYPVP 60
           MALF++LHP FSI  KTFT+FP  K FCFHRFK V NSM D SEP T NCP+  +NYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESVLASVP 120
           LSPPL AISKNLELARAM ASSKSSL+ALSA+DVIYEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180
           RLLGDSA AG     IK S+PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 121 RLLGDSANAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180

Query: 181 SYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240
           SYIA CVG++PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV
Sbjct: 181 SYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240

Query: 241 NGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGI 300
           NG++TMEEL + R+ EEETIVV TKSL+DID+ KDEILIRARPRSGRTHQIRLHCQYLGI
Sbjct: 241 NGKNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGI 300

Query: 301 PIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWASQALQPQQ-Q 360
           PIRGDVKYEGVTEWN KI D HELHAESLY  HPVTGIPLKLQAPLPSWASQALQPQQ +
Sbjct: 301 PIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHE 360

Query: 361 VNSPSSLKPMP 369
           VNSP S K MP
Sbjct: 361 VNSPQSFKTMP 366

[Close]

BLAST of Spg003579 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 5.0e-162
Identity = 295/330 (89.39%), Postives = 307/330 (93.03%), Query Frame = 0


Query: 37  MADPSEPATRNCPTAGDNYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYED 96
           MADPS P T N P  G NYP+PLSPPL AISKNLELARAMAASSKSSLFALSA+DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 97  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMV 156
           EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG++S  I+IS+PELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 157 ITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 216
           ITKSHKVASKLVKAFTDH VSKSYIA CVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 217 GRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEIL 276
           GRSLPGGSVVRDMETYFEVLSVNGQSTM+ELPE RKDEEETIVVQ+KSLIDIDA KDEIL
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 277 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGI 336
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG I D HELHAESL+  HPVTGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 337 PLKLQAPLPSWASQALQPQQQ-VNSPSSLK 366
           PLKLQAPLPSWASQAL  QQQ V+SPS+LK
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLK 330

[Close]

BLAST of Spg003579 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.8e-160
Identity = 296/340 (87.06%), Postives = 308/340 (90.59%), Query Frame = 0


Query: 37  MADPSEPATRNCPTAGDNYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYED 96
           MADPS P T N P  G NYP+PLSPPL AISKNLELARAMAASSKSSLFALSA+DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 97  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRS----------AEIKISVPELHLANR 156
           EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG++S          A I+IS+PELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 157 LDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFG 216
           LDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIA CVGSAPKWKKINVKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 217 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLI 276
           VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTM+ELPE RKDEEETIVVQ+KSLI
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 277 DIDAQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAES 336
           DIDA KDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG I D HELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 337 LYLAHPVTGIPLKLQAPLPSWASQALQPQQQ-VNSPSSLK 366
           L+  HPVTGIPLKLQAPLPSWASQAL  QQQ V+SPS+LK
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLK 340

[Close]

BLAST of Spg003579 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.7e-158
Identity = 285/333 (85.59%), Postives = 305/333 (91.59%), Query Frame = 0


Query: 37  MADPSEPATRNCPTAGDNYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYED 96
           M D SEP T NCP+ G+NYPVPLSPPL AISKNLELARAMAASSKSSLFALSA+DVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 97  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMV 156
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AG     IK ++PELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG-----IKTNLPELHLANRLDRDTSGVMV 120

Query: 157 ITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 216
           ITKSHKVASKLVKAFTDH VSKSYIA CVG++PKWKKI+VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 180

Query: 217 GRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEIL 276
           GRSLPGGS+VRDMETYFEVLSVNG++TMEEL + RK+EEETIVV TKSL+DID+QKDE+L
Sbjct: 181 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 240

Query: 277 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGI 336
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK  D HELHAESLY  HP+TGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 300

Query: 337 PLKLQAPLPSWASQALQPQQQ-VNSPSSLKPMP 369
           PLKLQAPLPSWASQALQPQQQ VNSP S K MP
Sbjct: 301 PLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of Spg003579 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.7e-158
Identity = 285/333 (85.59%), Postives = 305/333 (91.59%), Query Frame = 0


Query: 37  MADPSEPATRNCPTAGDNYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYED 96
           M D SEP T NCP+ G+NYPVPLSPPL AISKNLELARAMAASSKSSLFALSA+DVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 97  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMV 156
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AG     IK ++PELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG-----IKTNLPELHLANRLDRDTSGVMV 120

Query: 157 ITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 216
           ITKSHKVASKLVKAFTDH VSKSYIA CVG++PKWKKI+VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 180

Query: 217 GRSLPGGSVVRDMETYFEVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDEIL 276
           GRSLPGGS+VRDMETYFEVLSVNG++TMEEL + RK+EEETIVV TKSL+DID+QKDE+L
Sbjct: 181 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 240

Query: 277 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGI 336
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK  D HELHAESLY  HP+TGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 300

Query: 337 PLKLQAPLPSWASQALQPQQQ-VNSPSSLKPMP 369
           PLKLQAPLPSWASQALQPQQQ VNSP S K MP
Sbjct: 301 PLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of Spg003579 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.7e-99
Identity = 185/298 (62.08%), Postives = 223/298 (74.83%), Query Frame = 0


Query: 54  NYPVPLSPPLQAISKNLELARAMAASSKSSLFALSASDVIYEDEWLIAVNKPQGVYCESV 113
           NYP P+S P   ISK++EL RAM ASSKSSLF L+  D++YEDE+L+AVNKP+GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 114 LASVPRLLGDSAMAGDRSAEIKISVPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTD 173
           L S P+++ DS+              E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+
Sbjct: 99  LRSAPQIVLDSS-------------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTE 158

Query: 174 HKVSKSYIALCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYF 233
           HK+ KSYIALC+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET F
Sbjct: 159 HKIRKSYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTF 218

Query: 234 EVLSVNGQSTMEELPESRKDEEETIVVQTKSLIDIDAQKDE--ILIRARPRSGRTHQIRL 293
           EV+SVN  S   E  E  +D    IV + +  +      D+  +++RA PRSGRTHQIRL
Sbjct: 219 EVVSVN--SVKNESCE-LEDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRL 278

Query: 294 HCQYLGIPIRGDVKYEGVTEWNGKICDGHELHAESLYLAHPVTGIPLKLQAPLPSWAS 350
           HCQYLGIPIRGDVKY GV EWNG+  +GHELHAE L L HPVTG  + ++APLP WA+
Sbjct: 279 HCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of Spg003579 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.7e-11
Identity = 74/304 (24.34%), Postives = 112/304 (36.84%), Query Frame = 0


Query: 91  DVIYEDEWLIAVNK------------PQGVYCESVL--ASVPRLLGDSAMAGDRSAEIKI 150
           D++YED+ ++ VNK            P G     +L   S+P +   ++   D S E   
Sbjct: 149 DIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETF 208

Query: 151 SVPE--------------LHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIA 210
           S  E                + +RLD+ T+G++V+ K     + L + F  H + + Y++
Sbjct: 209 SDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVS 268

Query: 211 LCVG-SAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQ 270
           L  G  +P   +I +  G   S     R+  AA     +PGG              V G 
Sbjct: 269 LTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-----IPGG--------------VRGG 328

Query: 271 STMEELPESRKDEEETIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGIPIR 330
                                  +I+  A     L+  R  +GRTHQIR H +Y+G+P+ 
Sbjct: 329 RARH-------------AASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLL 388

Query: 331 GDVKYEGVTEWNGKI-------------------CDGHELHAESLYLAHPVTGIPLKLQA 347
           GD  Y G       +                    D   LHA  L   HP TG  +K   
Sbjct: 389 GDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSC 416

[Close]

BLAST of Spg003579 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.7e-09
Identity = 60/230 (26.09%), Postives = 98/230 (42.61%), Query Frame = 0


Query: 91  DVIYEDEWLIAVNKPQGVYC--------ESVLASVPRLLG-DSAMAGDRSAEIKISVPEL 150
           +V+YED+ LIA+NKP G+           +VL  +    G + +  G R +   + V   
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPV--- 157

Query: 151 HLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHG 210
              +RL R TSG+++  K+    +KL   F +           VGS      ++ + G G
Sbjct: 158 ---HRLGRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGTG 217

Query: 211 RSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV-----NGQSTMEELPESRKDEEE 270
           R           + + R+L  G V  D     + + V       Q      PE +    +
Sbjct: 218 RK---------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSK 277

Query: 271 TIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 307
             V++       D +K+  L++   +SGR HQIR+H  Y+G P+ GD  Y
Sbjct: 278 VFVLER------DREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]

BLAST of Spg003579 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-07
Identity = 57/224 (25.45%), Postives = 95/224 (42.41%), Query Frame = 0


Query: 91  DVIYEDEWLIAVNKPQGVYC--------ESVLASVPRLLG-DSAMAGDRSAEIKISVPEL 150
           +V+YED+ LIA+NKP G+           +VL  +    G + +  G R +   + V   
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPV--- 157

Query: 151 HLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHG 210
              +RL R TSG+++  K+    +KL   F +           VGS      ++ + G G
Sbjct: 158 ---HRLGRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGTG 217

Query: 211 RSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV-----NGQSTMEELPESRKDEEE 270
           R           + + R+L  G V  D     + + V       Q      PE +    +
Sbjct: 218 RK---------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSK 277

Query: 271 TIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGIPI 301
             V++       D +K+  L++   +SGR HQIR+H  Y+G P+
Sbjct: 278 VFVLER------DREKNCSLVKVEIQSGRPHQIRIHLAYMGHPL 289

[Close]

BLAST of Spg003579 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-07
Identity = 57/224 (25.45%), Postives = 95/224 (42.41%), Query Frame = 0


Query: 91  DVIYEDEWLIAVNKPQGVYC--------ESVLASVPRLLG-DSAMAGDRSAEIKISVPEL 150
           +V+YED+ LIA+NKP G+           +VL  +    G + +  G R +   + V   
Sbjct: 128 EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPV--- 187

Query: 151 HLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIALCVGSAPKWKKINVKSGHG 210
              +RL R TSG+++  K+    +KL   F +           VGS      ++ + G G
Sbjct: 188 ---HRLGRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGTG 247

Query: 211 RSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV-----NGQSTMEELPESRKDEEE 270
           R           + + R+L  G V  D     + + V       Q      PE +    +
Sbjct: 248 RK---------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSK 307

Query: 271 TIVVQTKSLIDIDAQKDEILIRARPRSGRTHQIRLHCQYLGIPI 301
             V++       D +K+  L++   +SGR HQIR+H  Y+G P+
Sbjct: 308 VFVLER------DREKNCSLVKVEIQSGRPHQIRIHLAYMGHPL 319

The following BLAST results are available for this feature:

BLAST of Spg003579 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021056.1	1.2e-173	86.33	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
XP_004144814.3	8.5e-172	84.10	RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ...	[more]
KAG6586224.1	2.5e-171	85.07	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038889468.1	9.3e-171	85.36	RNA pseudouridine synthase 1 [Benincasa hispida]	[more]
XP_022156345.1	1.0e-161	89.39	RNA pseudouridine synthase 1 isoform X2 [Momordica charantia]	[more]

BLAST of Spg003579 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XA65	3.8e-98	62.08	RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S...	[more]
Q2QNM3	2.4e-97	59.87	RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05...	[more]
Q68XB2	5.6e-17	31.06	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC...	[more]
Q8PHN2	2.1e-16	27.07	Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. c...	[more]
Q9ZDR7	2.8e-16	31.06	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai...	[more]

BLAST of Spg003579 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJY6	4.1e-172	84.10	PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03...	[more]
A0A6J1DQ12	5.0e-162	89.39	RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1DT56	2.8e-160	87.06	RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5A7TX25	5.7e-158	85.59	RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BW87	5.7e-158	85.59	RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1	[more]

BLAST of Spg003579 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56345.1	2.7e-99	62.08	Pseudouridine synthase family protein	[more]
AT1G76050.2	2.7e-11	24.34	Pseudouridine synthase family protein	[more]
AT3G52260.2	5.7e-09	26.09	Pseudouridine synthase family protein	[more]
AT3G52260.1	1.4e-07	25.45	Pseudouridine synthase family protein	[more]
AT3G52260.3	1.4e-07	25.45	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 87..345 e-value: 9.7E-45 score: 154.8
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 49..355
None	No IPR available	PANTHER	PTHR21600:SF47	RNA PSEUDOURIDINE SYNTHASE 1	coord: 49..355
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 99..294 e-value: 5.3E-23 score: 81.9
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 99..329 e-value: 7.45069E-42 score: 142.862
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 144..158
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 90..353

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003579.1	Spg003579.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding