Spg003561 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003561
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAmino_oxidase domain-containing protein
Locationscaffold4: 48912752 .. 48914155 (-)
RNA-Seq ExpressionSpg003561
SyntenySpg003561
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGAGAGAATAGAGATGGGTGCCACGTGGATCCACGGCATCGGCGGCAGCCCAATTCATAAAATCGCCGATCAAATCAATGCCCTTCACTCCGACCAGCCGTGGGAGTGCATGGACGGCTACTCCGATCACCCCACCTCTGTCGCAGAAGGCGGCGTCGAGCTCCCTCCGGCCACCTTCGACTCCATTTCCACTCTCTTCAAAAACCTCATGGATTTCGCTCAGGGAAAAATCTCCGGCGACTCCAACATTCCCCGGCAAGCAAATTCCGACAACCGCAGCATCGGAGGTTTTCTCCGGCGAGGGCTCGATTTGTACTGGCAGTCTAGAAATGGCGAAATCGAGGTTAATGGGTGTCAAGAATGGACCAAAAAATCGCTCGAGGAAGCCATTTTCGCCATGTTTGAAAACACCCAAAGAACTTACACCTCCGCCGGCGAGCTCTCGACGTTGGATTTCATTTCAGAAAGTGAATACCAAATGTTTCCCGGCGAGGAAATCACCATCGCCAAAGGCTATTTCAGCGTAATCCAATCAATCGCCTCTGTTCTTCCTCCCGGGTTAGTCCAATTGGGCAAAAAAGTCACCAAAATCGAATGGCATCCCGAACCAGACGGCCGGAATATTCCAACGCCGGTGAGACTGCATTTCTCCGACGGGTCCGACATGTCGGCCGATCACGTAATCGTCACCGTTTCACTCGGCGTTCTCAAAGCTGCAACTCAGCAAGATTCCGGTATGTTCAATCCGCCGTTGCCGTCGTTCAAAACAGAGGCGATTTCCCGGCTCGGATTTGGCGTCGTCAACAAGCTGTTTCTCCAGCTAGCTCCGGCCGCCGCCGACGGCGAAGATTTGGAGAGTAGAAACAAATTTCCTGGCCTGAATTTCGTTTTTCACAGGCCGGATTCGGAATTCCGGCGGAAGAAAATCCCATGGTGGATGAGAAGAACCACATCTCTCCGGCCAATCTACCAAAATTCGCCGATCTTACTATCTTGGTTCGCCGGAGAAGAAGCTCTTCATCTCGAGAAACTGAACGACGAGGAAATCATCAATGGAGTTTCGACAACCATCAGCAACTTCCTGATTCCAAATTCAGAATCAGATTGGAACAACGGGGAAAGAAACGTGAAATTGACAAACGTGTTGAAGAGCCAATGGGGCAGCGATCCTCTGTTTCTGGGGTCGTACTCGTACGTTGCGGTGGGATCGAGCGGCGAAGATTTGGACGCCATGGCAGAGCCATTGCCAAGAACAGAGGAAGGTTCAGAAGCTCCATTGCTTCAGATTCTGTTTGCAGGTGAAGCAACTCACAGAACCCATTATTCCACTACACATGGAGCTTATTTCAGTGGCCTTAGAGAGGCCAATAGATTGCTTCATCATTACAATTGCATTTGA

mRNA sequence

ATGGGCGAGAGAATAGAGATGGGTGCCACGTGGATCCACGGCATCGGCGGCAGCCCAATTCATAAAATCGCCGATCAAATCAATGCCCTTCACTCCGACCAGCCGTGGGAGTGCATGGACGGCTACTCCGATCACCCCACCTCTGTCGCAGAAGGCGGCGTCGAGCTCCCTCCGGCCACCTTCGACTCCATTTCCACTCTCTTCAAAAACCTCATGGATTTCGCTCAGGGAAAAATCTCCGGCGACTCCAACATTCCCCGGCAAGCAAATTCCGACAACCGCAGCATCGGAGGTTTTCTCCGGCGAGGGCTCGATTTGTACTGGCAGTCTAGAAATGGCGAAATCGAGGTTAATGGGTGTCAAGAATGGACCAAAAAATCGCTCGAGGAAGCCATTTTCGCCATGTTTGAAAACACCCAAAGAACTTACACCTCCGCCGGCGAGCTCTCGACGTTGGATTTCATTTCAGAAAGTGAATACCAAATGTTTCCCGGCGAGGAAATCACCATCGCCAAAGGCTATTTCAGCGTAATCCAATCAATCGCCTCTGTTCTTCCTCCCGGGTTAGTCCAATTGGGCAAAAAAGTCACCAAAATCGAATGGCATCCCGAACCAGACGGCCGGAATATTCCAACGCCGGTGAGACTGCATTTCTCCGACGGGTCCGACATGTCGGCCGATCACGTAATCGTCACCGTTTCACTCGGCGTTCTCAAAGCTGCAACTCAGCAAGATTCCGGTATGTTCAATCCGCCGTTGCCGTCGTTCAAAACAGAGGCGATTTCCCGGCTCGGATTTGGCGTCGTCAACAAGCTGTTTCTCCAGCTAGCTCCGGCCGCCGCCGACGGCGAAGATTTGGAGAGTAGAAACAAATTTCCTGGCCTGAATTTCGTTTTTCACAGGCCGGATTCGGAATTCCGGCGGAAGAAAATCCCATGGTGGATGAGAAGAACCACATCTCTCCGGCCAATCTACCAAAATTCGCCGATCTTACTATCTTGGTTCGCCGGAGAAGAAGCTCTTCATCTCGAGAAACTGAACGACGAGGAAATCATCAATGGAGTTTCGACAACCATCAGCAACTTCCTGATTCCAAATTCAGAATCAGATTGGAACAACGGGGAAAGAAACGTGAAATTGACAAACGTGTTGAAGAGCCAATGGGGCAGCGATCCTCTGTTTCTGGGGTCGTACTCGTACGTTGCGGTGGGATCGAGCGGCGAAGATTTGGACGCCATGGCAGAGCCATTGCCAAGAACAGAGGAAGGTTCAGAAGCTCCATTGCTTCAGATTCTGTTTGCAGGTGAAGCAACTCACAGAACCCATTATTCCACTACACATGGAGCTTATTTCAGTGGCCTTAGAGAGGCCAATAGATTGCTTCATCATTACAATTGCATTTGA

Coding sequence (CDS)

ATGGGCGAGAGAATAGAGATGGGTGCCACGTGGATCCACGGCATCGGCGGCAGCCCAATTCATAAAATCGCCGATCAAATCAATGCCCTTCACTCCGACCAGCCGTGGGAGTGCATGGACGGCTACTCCGATCACCCCACCTCTGTCGCAGAAGGCGGCGTCGAGCTCCCTCCGGCCACCTTCGACTCCATTTCCACTCTCTTCAAAAACCTCATGGATTTCGCTCAGGGAAAAATCTCCGGCGACTCCAACATTCCCCGGCAAGCAAATTCCGACAACCGCAGCATCGGAGGTTTTCTCCGGCGAGGGCTCGATTTGTACTGGCAGTCTAGAAATGGCGAAATCGAGGTTAATGGGTGTCAAGAATGGACCAAAAAATCGCTCGAGGAAGCCATTTTCGCCATGTTTGAAAACACCCAAAGAACTTACACCTCCGCCGGCGAGCTCTCGACGTTGGATTTCATTTCAGAAAGTGAATACCAAATGTTTCCCGGCGAGGAAATCACCATCGCCAAAGGCTATTTCAGCGTAATCCAATCAATCGCCTCTGTTCTTCCTCCCGGGTTAGTCCAATTGGGCAAAAAAGTCACCAAAATCGAATGGCATCCCGAACCAGACGGCCGGAATATTCCAACGCCGGTGAGACTGCATTTCTCCGACGGGTCCGACATGTCGGCCGATCACGTAATCGTCACCGTTTCACTCGGCGTTCTCAAAGCTGCAACTCAGCAAGATTCCGGTATGTTCAATCCGCCGTTGCCGTCGTTCAAAACAGAGGCGATTTCCCGGCTCGGATTTGGCGTCGTCAACAAGCTGTTTCTCCAGCTAGCTCCGGCCGCCGCCGACGGCGAAGATTTGGAGAGTAGAAACAAATTTCCTGGCCTGAATTTCGTTTTTCACAGGCCGGATTCGGAATTCCGGCGGAAGAAAATCCCATGGTGGATGAGAAGAACCACATCTCTCCGGCCAATCTACCAAAATTCGCCGATCTTACTATCTTGGTTCGCCGGAGAAGAAGCTCTTCATCTCGAGAAACTGAACGACGAGGAAATCATCAATGGAGTTTCGACAACCATCAGCAACTTCCTGATTCCAAATTCAGAATCAGATTGGAACAACGGGGAAAGAAACGTGAAATTGACAAACGTGTTGAAGAGCCAATGGGGCAGCGATCCTCTGTTTCTGGGGTCGTACTCGTACGTTGCGGTGGGATCGAGCGGCGAAGATTTGGACGCCATGGCAGAGCCATTGCCAAGAACAGAGGAAGGTTCAGAAGCTCCATTGCTTCAGATTCTGTTTGCAGGTGAAGCAACTCACAGAACCCATTATTCCACTACACATGGAGCTTATTTCAGTGGCCTTAGAGAGGCCAATAGATTGCTTCATCATTACAATTGCATTTGA

Protein sequence

MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPATFDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGCQEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQSIASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFHRPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTISNFLIPNSESDWNNGERNVKLTNVLKSQWGSDPLFLGSYSYVAVGSSGEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNCI
Homology
BLAST of Spg003561 vs. NCBI nr
Match: XP_022999455.1 (probable polyamine oxidase 5 [Cucurbita maxima])

HSP 1 Score: 785.8 bits (2028), Expect = 2.1e-223
Identity = 389/481 (80.87%), Postives = 423/481 (87.94%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPIH+IAD+I AL+SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 86  MGERIEMGATWIHGIGGSPIHEIADRIGALNSDQPWECMDGYSGQSTTVAEGGVELSPAT 145

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
            D ISTLFKNLMDFAQGKI+G+SNI  Q N D  S+G FLR+GL+ YW SRNG  EVNGC
Sbjct: 146 VDPISTLFKNLMDFAQGKIAGESNILHQGNYDKLSVGAFLRQGLESYWLSRNGVTEVNGC 205

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
            EW++K+LEEAIFAM+EN QRTYTSAGELSTLDF++ESEYQMFPGEEITIAKGY SVI+S
Sbjct: 206 GEWSQKALEEAIFAMYENNQRTYTSAGELSTLDFVAESEYQMFPGEEITIAKGYLSVIES 265

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEW+PEP+ RNIPTPV LHF+DGS  SADHVIVTVSLGVLKA
Sbjct: 266 IASVLPPGLVQLGKKVTKIEWNPEPESRNIPTPVTLHFADGSHTSADHVIVTVSLGVLKA 325

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            T++DS +FNPPLPSFKTEAISRLGFGVVNKLFL+L+  A DG++ +S NKFP LNFVFH
Sbjct: 326 GTREDSALFNPPLPSFKTEAISRLGFGVVNKLFLRLSQPANDGDNPKSNNKFPCLNFVFH 385

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDSEFRRKKIPWWMR+ T+L+PIYQNS ILLSWFAGEEAL LEKL DEEIINGVSTTIS
Sbjct: 386 QPDSEFRRKKIPWWMRKITTLQPIYQNSSILLSWFAGEEALQLEKLKDEEIINGVSTTIS 445

Query: 361 NFLIP-----NSESD---WNNGERN------VKLTNVLKSQWGSDPLFLGSYSYVAVGSS 420
           NFLIP     NSESD   W+NG RN       +L  VLKSQWGSDPLF GSYSYVAVGSS
Sbjct: 446 NFLIPRSQNENSESDSNGWSNGHRNRNDTVEFQLNQVLKSQWGSDPLFQGSYSYVAVGSS 505

Query: 421 GEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 468
           GEDLDAMAEPLPRTEE  +APLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC
Sbjct: 506 GEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 565

BLAST of Spg003561 vs. NCBI nr
Match: XP_008459345.1 (PREDICTED: probable polyamine oxidase 5 [Cucumis melo] >KAA0061493.1 putative polyamine oxidase 5 [Cucumis melo var. makuwa] >TYK10780.1 putative polyamine oxidase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 782.3 bits (2019), Expect = 2.3e-222
Identity = 390/479 (81.42%), Postives = 423/479 (88.31%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPI+KIADQI AL SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 55  MGERIEMGATWIHGIGGSPIYKIADQIGALQSDQPWECMDGYSGQSTTVAEGGVELSPAT 114

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
              ISTLF+ LMDFAQGKI+GDSNI +QAN D RSIG FLR+GLD YW S+NGE E+NGC
Sbjct: 115 VKPISTLFQKLMDFAQGKITGDSNILQQANYDKRSIGDFLRQGLDSYWVSKNGETELNGC 174

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
           +EW++KSLEEA+FAM+EN QRTYTSAG+LSTLDFISESEYQMFPGEEITIAKGY SVI+S
Sbjct: 175 KEWSQKSLEEAVFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIES 234

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEWHPE D RNIPTPV LHF+DGS +SADHVIVTVSLGVLKA
Sbjct: 235 IASVLPPGLVQLGKKVTKIEWHPELDPRNIPTPVTLHFADGSHISADHVIVTVSLGVLKA 294

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            TQ  S +F+PPLPSFKTEAISRLGFGVVNKLFL+LAP   +G DL+  ++FP L FVFH
Sbjct: 295 GTQPVSPLFHPPLPSFKTEAISRLGFGVVNKLFLRLAPVTDNGHDLKRTHQFPCLKFVFH 354

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDS+FRRKKIPWWMR+TT+LRPIYQNS ILLSW AGEEALHLEKL DEEIINGVSTTIS
Sbjct: 355 QPDSKFRRKKIPWWMRKTTTLRPIYQNSSILLSWLAGEEALHLEKLKDEEIINGVSTTIS 414

Query: 361 NFLI-----PNSESDWN-----NGERNVK--LTNVLKSQWGSDPLFLGSYSYVAVGSSGE 420
           NFLI      NSESD N     NG RN++   + VLKSQWGSDPLFLGSYSYVAVGSSGE
Sbjct: 415 NFLIQKSESENSESDSNCCNKMNGHRNIEFSFSQVLKSQWGSDPLFLGSYSYVAVGSSGE 474

Query: 421 DLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNCI 468
           DLDAMAEPLPRTEE S+APLLQILFAGEATHRTHYSTTHGAY+SGLREANRLLHHYNCI
Sbjct: 475 DLDAMAEPLPRTEESSKAPLLQILFAGEATHRTHYSTTHGAYYSGLREANRLLHHYNCI 533

BLAST of Spg003561 vs. NCBI nr
Match: XP_038889398.1 (probable polyamine oxidase 5 [Benincasa hispida])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 385/483 (79.71%), Postives = 422/483 (87.37%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSP++KIAD+I ALHSDQPWECMDGYS   T+VAEGGV+L PAT
Sbjct: 101 MGERIEMGATWIHGIGGSPLYKIADEIGALHSDQPWECMDGYSGQSTTVAEGGVDLSPAT 160

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
            D IS LF+NLMDFAQGKI+GDSNI +Q+N D  S+G FLRRGLD YW S+NG+ E+NGC
Sbjct: 161 VDPISALFQNLMDFAQGKITGDSNIFQQSNYDKLSVGDFLRRGLDSYWLSKNGQTELNGC 220

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
           +EW++KSLEEAIFAM+EN QRTYTSAG+LSTLDFISESEYQMFPGEEITIAKGY SVI+S
Sbjct: 221 KEWSQKSLEEAIFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIES 280

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEWHPEP+ RNIPTPV LHF+DGS +SADHVI+TVSLGVLKA
Sbjct: 281 IASVLPPGLVQLGKKVTKIEWHPEPNPRNIPTPVTLHFADGSHISADHVIITVSLGVLKA 340

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            TQ DS +F+PPLPSFK +AISRLGFGVVNKLFL+LAP   +G+DL+  ++FP LNFVFH
Sbjct: 341 GTQPDSALFHPPLPSFKRQAISRLGFGVVNKLFLRLAPVTDNGQDLKRTHQFPCLNFVFH 400

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDSEFRRKKIPWWMRRTT+LRPIYQNS ILLSW AGEEALHLEKL D+EIINGVSTTI 
Sbjct: 401 QPDSEFRRKKIPWWMRRTTTLRPIYQNSSILLSWLAGEEALHLEKLKDDEIINGVSTTIR 460

Query: 361 NFLI-----PNSESDWN-----NGERNV------KLTNVLKSQWGSDPLFLGSYSYVAVG 420
           NFLI      N ESD       NG RN         + VLKSQWGSDPLFLGSYSYVAVG
Sbjct: 461 NFLIHKSQNENLESDSTCCNNINGHRNTNENVEFSFSQVLKSQWGSDPLFLGSYSYVAVG 520

Query: 421 SSGEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHY 468
           SSGEDLDAMAEPLPRTEE S+APLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHY
Sbjct: 521 SSGEDLDAMAEPLPRTEESSKAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHY 580

BLAST of Spg003561 vs. NCBI nr
Match: KAG6599509.1 (putative polyamine oxidase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 779.6 bits (2012), Expect = 1.5e-221
Identity = 385/481 (80.04%), Postives = 422/481 (87.73%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPI++IAD+I AL+SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 55  MGERIEMGATWIHGIGGSPIYEIADRIGALNSDQPWECMDGYSGQSTTVAEGGVELSPAT 114

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
            D ISTLFKNLMDFAQGKI+G+SN  RQ N D  S+G FLRRGL+ YW S+NG  EVNGC
Sbjct: 115 VDPISTLFKNLMDFAQGKIAGESNFLRQGNYDKLSVGAFLRRGLESYWLSKNGVTEVNGC 174

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
            EW++K+LEEAIFAM+EN QRTYTSAGELSTLDF++ESEYQMFPGEEITIAKGY SVI+S
Sbjct: 175 GEWSQKALEEAIFAMYENNQRTYTSAGELSTLDFVAESEYQMFPGEEITIAKGYLSVIES 234

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEW+PE + RNIPTPV LHF+DGS  SADHVIVTVSLGVLKA
Sbjct: 235 IASVLPPGLVQLGKKVTKIEWNPESESRNIPTPVTLHFADGSHTSADHVIVTVSLGVLKA 294

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            T++DS +FNPPLPSFKTEAISRLGFGVVNKLFL+L+P   DG++ +S NKFP LNFVFH
Sbjct: 295 GTREDSALFNPPLPSFKTEAISRLGFGVVNKLFLRLSPPTNDGDNPKSINKFPCLNFVFH 354

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDSEFRRK+IPWWMR+TT+L+PIYQNS ILLSWFAGEEAL LEKL D+EIINGVSTTIS
Sbjct: 355 QPDSEFRRKRIPWWMRKTTTLQPIYQNSSILLSWFAGEEALQLEKLKDDEIINGVSTTIS 414

Query: 361 NFLIP-----NSESD---WNNGERN------VKLTNVLKSQWGSDPLFLGSYSYVAVGSS 420
           NFLIP     NSESD   W+NG RN         + VLKSQWGSDPLF GSYSYVAVGSS
Sbjct: 415 NFLIPRSQNENSESDSNGWSNGHRNQNDTVEFHFSQVLKSQWGSDPLFQGSYSYVAVGSS 474

Query: 421 GEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 468
           GEDLDAMAEPLPRTEE  +APLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC
Sbjct: 475 GEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 534

BLAST of Spg003561 vs. NCBI nr
Match: XP_022945998.1 (probable polyamine oxidase 5 [Cucurbita moschata] >XP_022945999.1 probable polyamine oxidase 5 [Cucurbita moschata] >KAG7030485.1 putative polyamine oxidase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 778.9 bits (2010), Expect = 2.5e-221
Identity = 384/481 (79.83%), Postives = 423/481 (87.94%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPI++IAD+I AL+SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 55  MGERIEMGATWIHGIGGSPIYEIADRIGALNSDQPWECMDGYSGQSTTVAEGGVELSPAT 114

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
            D ISTLFKNLMDFAQGKI+G+SN  R+ N D  S+G FLRRGL+ YW S+NG  EVNGC
Sbjct: 115 VDPISTLFKNLMDFAQGKIAGESNFLRRGNYDKLSVGAFLRRGLESYWLSKNGVTEVNGC 174

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
            EW++K+LEEAIFAM+EN QRTYTSAGELSTLDF++ESEYQMFPGEEITIAKGY SVI+S
Sbjct: 175 GEWSQKALEEAIFAMYENNQRTYTSAGELSTLDFVAESEYQMFPGEEITIAKGYLSVIES 234

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEW+PE + RNIPTPV LHF+DGS  SADHVIVTVSLGVLKA
Sbjct: 235 IASVLPPGLVQLGKKVTKIEWNPESESRNIPTPVTLHFADGSHTSADHVIVTVSLGVLKA 294

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            T++DS +FNPPLPSFKTEAISRLGFGVVNKLFL+L+P   DG++ +S NKFP LNFVFH
Sbjct: 295 GTREDSALFNPPLPSFKTEAISRLGFGVVNKLFLRLSPPTNDGDNPKSINKFPCLNFVFH 354

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDSEFRRK+IPWWMR+TT+L+PIYQNS ILLSWFAGEEAL LEKL D+EIINGVSTTIS
Sbjct: 355 QPDSEFRRKRIPWWMRKTTTLQPIYQNSSILLSWFAGEEALQLEKLKDDEIINGVSTTIS 414

Query: 361 NFLIP-----NSESD---WNNGERN------VKLTNVLKSQWGSDPLFLGSYSYVAVGSS 420
           NFLIP     NSESD   W+NG+RN         + VLKSQWGSDPLF GSYSYVAVGSS
Sbjct: 415 NFLIPRSQNENSESDSNGWSNGQRNQNDTVEFHFSQVLKSQWGSDPLFQGSYSYVAVGSS 474

Query: 421 GEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 468
           GEDLDAMAEPLPRTEE  +APLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC
Sbjct: 475 GEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 534

BLAST of Spg003561 vs. ExPASy Swiss-Prot
Match: Q9SU79 (Probable polyamine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PAO5 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.8e-153
Identity = 286/497 (57.55%), Postives = 347/497 (69.82%), Query Frame = 0

Query: 3   ERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPATFD 62
           E+IEMGATWIHGIGGSP+++IA +  +L SD+PWECMD   D   + AEGG E+ P+  +
Sbjct: 54  EKIEMGATWIHGIGGSPVYRIAKETGSLVSDEPWECMDSTIDKAKTFAEGGFEIEPSIVE 113

Query: 63  SISTLFKNLMDFAQGK--ISGDSNIPRQAN-----------SDNRSIGGFLRRGLDLYWQ 122
           SIS LF  LM+ AQGK     D+++ R A+             + S+G FL+ G D YW 
Sbjct: 114 SISGLFTALMELAQGKEISQSDADLSRLAHIYETATRVCSKGSSTSVGSFLKSGFDAYWD 173

Query: 123 --SRNGEIEVNGCQEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEE 182
             S  GE  V G  +W++KSLEEAIF MF NTQRTYTSA ELSTLDF +ESEYQMFPGEE
Sbjct: 174 SISNGGEEGVKGYGKWSRKSLEEAIFTMFSNTQRTYTSADELSTLDFAAESEYQMFPGEE 233

Query: 183 ITIAKGYFSVIQSIASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSAD 242
           ITIAKGY SVI  +ASVLP G++QL +KVTKIEW            V+LHFSDGS + AD
Sbjct: 234 ITIAKGYLSVIHHLASVLPQGVIQLNRKVTKIEWQ--------SNEVKLHFSDGSVVFAD 293

Query: 243 HVIVTVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLE 302
           HVIVTVSLGVLKA  + D+ +F+PPLP FK++AI RLG+GVVNKLF+++           
Sbjct: 294 HVIVTVSLGVLKAGIETDAELFSPPLPDFKSDAIRRLGYGVVNKLFVEM----------- 353

Query: 303 SRNKFPGLNFVFHRPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLN 362
           S+ KFP L  VF R DSEFR  KIPWWMRRT ++ PI+ NS +LLSWFAG+EAL LEKL 
Sbjct: 354 SQRKFPSLQLVFDREDSEFRFVKIPWWMRRTATITPIHSNSKVLLSWFAGKEALELEKLT 413

Query: 363 DEEIINGVSTTIS---------NFLIPNSESDWNNGERNVKLTNVLKSQWGSDPLFLGSY 422
           DEEI + V TTIS         +   P +    N+ +  +K+T VLKS+WGSDPLF GSY
Sbjct: 414 DEEIKDAVMTTISCLTGKEVKNDTAKPLTNGSLNDDDEAMKITKVLKSKWGSDPLFRGSY 473

Query: 423 SYVAVGSSGEDLDAMAEPLPRT---------EEGSEAPLLQILFAGEATHRTHYSTTHGA 467
           SYVAVGSSG+DLDAMAEPLP+           + ++   LQ++FAGEATHRTHYSTTHGA
Sbjct: 474 SYVAVGSSGDDLDAMAEPLPKINKKVGQVNGHDQAKVHELQVMFAGEATHRTHYSTTHGA 531

BLAST of Spg003561 vs. ExPASy Swiss-Prot
Match: Q5NAI7 (Polyamine oxidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAO1 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 216/485 (44.54%), Postives = 300/485 (61.86%), Query Frame = 0

Query: 2   GERIEMGATWIHGIGGSPIHKIADQINALHSDQ----PWECMDGYSDHPTSVAEGGVELP 61
           G R+EMGATW+ G+ GSP++ +A    AL  ++    P+E MDG+ D   +VAEGG  + 
Sbjct: 54  GHRVEMGATWVQGVVGSPVYALARDAGALGEEEGRGLPYERMDGFPDRVLTVAEGGEVVD 113

Query: 62  PATF-DSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIE 121
             T    I  L++ +M+ A+   +G              +  +LRRGL  Y  +R+    
Sbjct: 114 ADTVAGPIEELYRGMMEAARAGEAGGGG----------GVEEYLRRGLRAYQAARSAGGG 173

Query: 122 VNGCQEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFS 181
             G +E   + ++EA+ AM  N +RT TSA +L  LD  +E EY+ FPGE +TI  GY  
Sbjct: 174 GGGGKE--LEEVDEALLAMHINRERTDTSADDLGDLDLTAEGEYRDFPGEHVTIPGGYSR 233

Query: 182 VIQSIASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGS-DMSADHVIVTVSL 241
           V++ +A+ LPPG V+LG ++ +++W          TPVRLHF+DG+  ++ADHVI+TVSL
Sbjct: 234 VVERLAAALPPGTVRLGLRLRRLKWG--------GTPVRLHFADGAPPLTADHVILTVSL 293

Query: 242 GVLKAA--TQQDSGM------FNPPLPSFKTEAISRLGFGVVNKLFLQL-APAAADGEDL 301
           GVLKA+   +  +G+      F+PPLP FK EA++RLGFGVVNKLF+++ A A ++ ED+
Sbjct: 294 GVLKASLGNKDTAGVGAAAIAFDPPLPPFKREAVARLGFGVVNKLFMEVEAVAPSEPEDV 353

Query: 302 ----ESRNKFPGLNFVFHRPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALH 361
                +   FP L+  F    S     KIPWWMR T S+ P++  S + L+WFAG EA H
Sbjct: 354 AGVQPAAAGFPFLHMAFRGHVS-----KIPWWMRGTESICPVHAGSTVALAWFAGREAAH 413

Query: 362 LEKLNDEEIINGVSTTISNFLIPNSESDWNNGERNVKLTNVLKSQWGSDPLFLGSYSYVA 421
           LE L D+++I G   T+ +FL   +   W       ++  + +S W +DPLFLGSYSYVA
Sbjct: 414 LESLPDDDVIRGAHATLDSFL--PAAPRW-------RVRRIKRSGWATDPLFLGSYSYVA 473

Query: 422 VGSSGEDLDAMAEPLPRTEEGS---EAPLLQILFAGEATHRTHYSTTHGAYFSGLREANR 465
           VGSSG+DLD MAEPLPR  + +     P  ++LFAGEATHRTHYSTTH AY SG+REANR
Sbjct: 474 VGSSGDDLDRMAEPLPRGPDAAADERPPSPRLLFAGEATHRTHYSTTHAAYLSGVREANR 504

BLAST of Spg003561 vs. ExPASy Swiss-Prot
Match: Q99K82 (Spermine oxidase OS=Mus musculus OX=10090 GN=Smox PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.6e-53
Identity = 164/504 (32.54%), Postives = 234/504 (46.43%), Query Frame = 0

Query: 6   EMGATWIHGIGGSPIHKIADQINALHSDQPWECMDG----YSDHPTS--VAEGGVELPPA 65
           E+GATWIHG  G+PI+++A+    L      E   G    YS +  +  +   G  +P  
Sbjct: 75  ELGATWIHGSHGNPIYQLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNRGCRIPKD 134

Query: 66  TFDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNG 125
             +  S L+  + +  Q         P  A S N S+G F R       + RN   +   
Sbjct: 135 VVEEFSDLYNEVYNMTQEFFRHGK--PVNAESQN-SVGVFTRE------KVRNRIRDDPD 194

Query: 126 CQEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQ 185
             E TK+ L+ A+   +   +   +S+  +  +   +  E+   PG    I  G+  V++
Sbjct: 195 DTEATKR-LKLAMIQQYLKVESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVE 254

Query: 186 SIASVLPPGLVQLGKKVTKIEW-----HP-----EPDG---------------------- 245
            +A  +PP ++QLGK V  I W     HP     EP G                      
Sbjct: 255 LLAEGIPPHVIQLGKPVRCIHWDQASAHPRGPEIEPRGEGDHNHDTGEGGQSGENPQQGR 314

Query: 246 --RNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLG 305
              + P PV +   D   + ADHVIVTVSLGVLK   +Q +  F P LP+ K  AI RLG
Sbjct: 315 WDEDEPWPVVVECEDCEVIPADHVIVTVSLGVLK---RQYTSFFRPCLPTEKVAAIHRLG 374

Query: 306 FGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFHRPDSEFRRKKIP-WWMRRTTSLRPI 365
            G  +K+FL+        E+     +   L FV+            P  W R+      +
Sbjct: 375 IGTTDKIFLEF-------EEPFWGPECNSLQFVWEDEAESCTLTYPPELWYRKICGFDVL 434

Query: 366 Y---QNSPILLSWFAGEEALHLEKLNDEEIINGVSTTISNFLIPNSESDWNNGERNV-KL 425
           Y   +   +L  W  GEEAL +E+ +DE +    +  +  F           G  N+ K 
Sbjct: 435 YPPERYGHVLSGWICGEEALVMERCDDEAVAEICTEMLRQF----------TGNPNIPKP 494

Query: 426 TNVLKSQWGSDPLFLGSYSYVAVGSSGEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHR 465
             +L+S WGS+P F GSYSY  VGSSG D++ +A+PLP TE    AP +Q+LF+GEATHR
Sbjct: 495 RRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAP-MQVLFSGEATHR 547

BLAST of Spg003561 vs. ExPASy Swiss-Prot
Match: Q9NWM0 (Spermine oxidase OS=Homo sapiens OX=9606 GN=SMOX PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.5e-51
Identity = 163/505 (32.28%), Postives = 231/505 (45.74%), Query Frame = 0

Query: 6   EMGATWIHGIGGSPIHKIADQINALHSDQPWECMDG----YSDHPTS--VAEGGVELPPA 65
           E+GATWIHG  G+PI+ +A+    L      E   G    YS +  +  +   G  +P  
Sbjct: 75  ELGATWIHGSHGNPIYHLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNHGRRIPKD 134

Query: 66  TFDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNG 125
             +  S L+  + +  Q     D   P  A S N S+G F R       + RN  I  + 
Sbjct: 135 VVEEFSDLYNEVYNLTQEFFRHDK--PVNAESQN-SVGVFTRE------EVRN-RIRNDP 194

Query: 126 CQEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQ 185
                 K L+ A+   +   +   +S+  +  +   +  E+   PG    I  G+  V++
Sbjct: 195 DDPEATKRLKLAMIQQYLKVESCESSSHSMDEVSLSAFGEWTEIPGAHHIIPSGFMRVVE 254

Query: 186 SIASVLPPGLVQLGKKVTKIEWHP----------EPDG---RNIPT-------------- 245
            +A  +P  ++QLGK V  I W            EP G    N  T              
Sbjct: 255 LLAEGIPAHVIQLGKPVRCIHWDQASARPRGPEIEPRGEGDHNHDTGEGGQGGEEPRGGR 314

Query: 246 -------PVRLHFSDGSDMSADHVIVTVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLG 305
                   V +   D   + ADHVIVTVSLGVLK   +Q +  F P LP+ K  AI RLG
Sbjct: 315 WDEDEQWSVVVECEDCELIPADHVIVTVSLGVLK---RQYTSFFRPGLPTEKVAAIHRLG 374

Query: 306 FGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFHRPDSEFRRKKIP--WWMRRTTSLRP 365
            G  +K+FL+        E+     +   L FV+   ++E      P   W R+      
Sbjct: 375 IGTTDKIFLEF-------EEPFWGPECNSLQFVW-EDEAESHTLTYPPELWYRKICGFDV 434

Query: 366 IY---QNSPILLSWFAGEEALHLEKLNDEEIINGVSTTISNFLIPNSESDWNNGERNV-K 425
           +Y   +   +L  W  GEEAL +EK +DE +    +  +  F           G  N+ K
Sbjct: 435 LYPPERYGHVLSGWICGEEALVMEKCDDEAVAEICTEMLRQF----------TGNPNIPK 494

Query: 426 LTNVLKSQWGSDPLFLGSYSYVAVGSSGEDLDAMAEPLPRTEEGSEAPLLQILFAGEATH 465
              +L+S WGS+P F GSYSY  VGSSG D++ +A+PLP TE    AP +Q+LF+GEATH
Sbjct: 495 PRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAP-MQVLFSGEATH 547

BLAST of Spg003561 vs. ExPASy Swiss-Prot
Match: Q8C0L6 (Peroxisomal N(1)-acetyl-spermine/spermidine oxidase OS=Mus musculus OX=10090 GN=Paox PE=1 SV=3)

HSP 1 Score: 198.7 bits (504), Expect = 1.4e-49
Identity = 153/476 (32.14%), Postives = 226/476 (47.48%), Query Frame = 0

Query: 2   GERIEMGATWIHGIG-GSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGG-VELPPA 61
           G  +E+GA WIHG    +P+ ++A +   L   +        S+    V  GG V LP  
Sbjct: 53  GGVVELGAHWIHGPSQDNPVFQLAAEFGLLGEKE-------LSEENQLVDTGGHVALPSM 112

Query: 62  TFDSIST-----LFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGE 121
            + S  T     L   +     G I        ++ +   S+G FL++ +     S   +
Sbjct: 113 IWSSSGTSVSLELMTEMARLFYGLIERTREFLNESETPMASVGEFLKKEISQQVASWTED 172

Query: 122 IEVNGCQEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGY 181
            E     +  K+ L  AI   F N +   +    +  +      EY + PG +  +A GY
Sbjct: 173 DE-----DTRKRKL--AILNTFFNIECCVSGTHSMDLVALAPFGEYTVLPGLDCILAGGY 232

Query: 182 FSVIQSIASVLPPGLVQLGKKVTKIEWHPEPDGRNIP---TPVRLHFSDGSDMSADHVIV 241
             +   I + LP   V   K V  I W+        P    PV +   DG+ + A HVIV
Sbjct: 233 QGLTDRILASLPKDTVAFDKPVKTIHWNGSFQEAAFPGETFPVLVECEDGARLPAHHVIV 292

Query: 242 TVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNK 301
           TV LG LK   +     F PPLP+ K EAI +LGFG  NK+FL+      +    E   +
Sbjct: 293 TVPLGFLK---EHQDTFFEPPLPAKKAEAIKKLGFGTNNKIFLEF-----EEPFWEPDCQ 352

Query: 302 FPGLNFVFHRPDSEFRRKKIPWWMRRTTS--LRPIYQNSPILLSWFAGEEALHLEKLNDE 361
           F  + +    P  +        W ++     ++P +++S +L  + AG E+  +E L+DE
Sbjct: 353 FIQVVWEDTSPLQDTALSLQDTWFKKLIGFLVQPSFESSHVLCGFIAGLESEFMETLSDE 412

Query: 362 EIINGVSTTI----SNFLIPNSESDWNNGERNVKLTNVLKSQWGSDPLFLGSYSYVAVGS 421
           E++  ++  +     N  +P ++S             V +SQW S P   GSYSYVAVGS
Sbjct: 413 EVLLSLTQVLRRVTGNPQLPAAKS-------------VRRSQWHSAPYTRGSYSYVAVGS 472

Query: 422 SGEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLL 462
           +G+DLD MA+PLP    G++   LQ+LFAGEATHRT YSTTHGA  SG REA+RL+
Sbjct: 473 TGDDLDLMAQPLPEDGTGTQ---LQVLFAGEATHRTFYSTTHGALLSGWREADRLV 490

BLAST of Spg003561 vs. ExPASy TrEMBL
Match: A0A6J1KFF8 (probable polyamine oxidase 5 OS=Cucurbita maxima OX=3661 GN=LOC111493815 PE=4 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 1.0e-223
Identity = 389/481 (80.87%), Postives = 423/481 (87.94%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPIH+IAD+I AL+SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 86  MGERIEMGATWIHGIGGSPIHEIADRIGALNSDQPWECMDGYSGQSTTVAEGGVELSPAT 145

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
            D ISTLFKNLMDFAQGKI+G+SNI  Q N D  S+G FLR+GL+ YW SRNG  EVNGC
Sbjct: 146 VDPISTLFKNLMDFAQGKIAGESNILHQGNYDKLSVGAFLRQGLESYWLSRNGVTEVNGC 205

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
            EW++K+LEEAIFAM+EN QRTYTSAGELSTLDF++ESEYQMFPGEEITIAKGY SVI+S
Sbjct: 206 GEWSQKALEEAIFAMYENNQRTYTSAGELSTLDFVAESEYQMFPGEEITIAKGYLSVIES 265

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEW+PEP+ RNIPTPV LHF+DGS  SADHVIVTVSLGVLKA
Sbjct: 266 IASVLPPGLVQLGKKVTKIEWNPEPESRNIPTPVTLHFADGSHTSADHVIVTVSLGVLKA 325

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            T++DS +FNPPLPSFKTEAISRLGFGVVNKLFL+L+  A DG++ +S NKFP LNFVFH
Sbjct: 326 GTREDSALFNPPLPSFKTEAISRLGFGVVNKLFLRLSQPANDGDNPKSNNKFPCLNFVFH 385

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDSEFRRKKIPWWMR+ T+L+PIYQNS ILLSWFAGEEAL LEKL DEEIINGVSTTIS
Sbjct: 386 QPDSEFRRKKIPWWMRKITTLQPIYQNSSILLSWFAGEEALQLEKLKDEEIINGVSTTIS 445

Query: 361 NFLIP-----NSESD---WNNGERN------VKLTNVLKSQWGSDPLFLGSYSYVAVGSS 420
           NFLIP     NSESD   W+NG RN       +L  VLKSQWGSDPLF GSYSYVAVGSS
Sbjct: 446 NFLIPRSQNENSESDSNGWSNGHRNRNDTVEFQLNQVLKSQWGSDPLFQGSYSYVAVGSS 505

Query: 421 GEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 468
           GEDLDAMAEPLPRTEE  +APLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC
Sbjct: 506 GEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 565

BLAST of Spg003561 vs. ExPASy TrEMBL
Match: A0A5A7UZX3 (Putative polyamine oxidase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00930 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.1e-222
Identity = 390/479 (81.42%), Postives = 423/479 (88.31%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPI+KIADQI AL SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 55  MGERIEMGATWIHGIGGSPIYKIADQIGALQSDQPWECMDGYSGQSTTVAEGGVELSPAT 114

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
              ISTLF+ LMDFAQGKI+GDSNI +QAN D RSIG FLR+GLD YW S+NGE E+NGC
Sbjct: 115 VKPISTLFQKLMDFAQGKITGDSNILQQANYDKRSIGDFLRQGLDSYWVSKNGETELNGC 174

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
           +EW++KSLEEA+FAM+EN QRTYTSAG+LSTLDFISESEYQMFPGEEITIAKGY SVI+S
Sbjct: 175 KEWSQKSLEEAVFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIES 234

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEWHPE D RNIPTPV LHF+DGS +SADHVIVTVSLGVLKA
Sbjct: 235 IASVLPPGLVQLGKKVTKIEWHPELDPRNIPTPVTLHFADGSHISADHVIVTVSLGVLKA 294

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            TQ  S +F+PPLPSFKTEAISRLGFGVVNKLFL+LAP   +G DL+  ++FP L FVFH
Sbjct: 295 GTQPVSPLFHPPLPSFKTEAISRLGFGVVNKLFLRLAPVTDNGHDLKRTHQFPCLKFVFH 354

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDS+FRRKKIPWWMR+TT+LRPIYQNS ILLSW AGEEALHLEKL DEEIINGVSTTIS
Sbjct: 355 QPDSKFRRKKIPWWMRKTTTLRPIYQNSSILLSWLAGEEALHLEKLKDEEIINGVSTTIS 414

Query: 361 NFLI-----PNSESDWN-----NGERNVK--LTNVLKSQWGSDPLFLGSYSYVAVGSSGE 420
           NFLI      NSESD N     NG RN++   + VLKSQWGSDPLFLGSYSYVAVGSSGE
Sbjct: 415 NFLIQKSESENSESDSNCCNKMNGHRNIEFSFSQVLKSQWGSDPLFLGSYSYVAVGSSGE 474

Query: 421 DLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNCI 468
           DLDAMAEPLPRTEE S+APLLQILFAGEATHRTHYSTTHGAY+SGLREANRLLHHYNCI
Sbjct: 475 DLDAMAEPLPRTEESSKAPLLQILFAGEATHRTHYSTTHGAYYSGLREANRLLHHYNCI 533

BLAST of Spg003561 vs. ExPASy TrEMBL
Match: A0A1S3CB61 (probable polyamine oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103498503 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.1e-222
Identity = 390/479 (81.42%), Postives = 423/479 (88.31%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPI+KIADQI AL SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 55  MGERIEMGATWIHGIGGSPIYKIADQIGALQSDQPWECMDGYSGQSTTVAEGGVELSPAT 114

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
              ISTLF+ LMDFAQGKI+GDSNI +QAN D RSIG FLR+GLD YW S+NGE E+NGC
Sbjct: 115 VKPISTLFQKLMDFAQGKITGDSNILQQANYDKRSIGDFLRQGLDSYWVSKNGETELNGC 174

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
           +EW++KSLEEA+FAM+EN QRTYTSAG+LSTLDFISESEYQMFPGEEITIAKGY SVI+S
Sbjct: 175 KEWSQKSLEEAVFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIES 234

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEWHPE D RNIPTPV LHF+DGS +SADHVIVTVSLGVLKA
Sbjct: 235 IASVLPPGLVQLGKKVTKIEWHPELDPRNIPTPVTLHFADGSHISADHVIVTVSLGVLKA 294

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            TQ  S +F+PPLPSFKTEAISRLGFGVVNKLFL+LAP   +G DL+  ++FP L FVFH
Sbjct: 295 GTQPVSPLFHPPLPSFKTEAISRLGFGVVNKLFLRLAPVTDNGHDLKRTHQFPCLKFVFH 354

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDS+FRRKKIPWWMR+TT+LRPIYQNS ILLSW AGEEALHLEKL DEEIINGVSTTIS
Sbjct: 355 QPDSKFRRKKIPWWMRKTTTLRPIYQNSSILLSWLAGEEALHLEKLKDEEIINGVSTTIS 414

Query: 361 NFLI-----PNSESDWN-----NGERNVK--LTNVLKSQWGSDPLFLGSYSYVAVGSSGE 420
           NFLI      NSESD N     NG RN++   + VLKSQWGSDPLFLGSYSYVAVGSSGE
Sbjct: 415 NFLIQKSESENSESDSNCCNKMNGHRNIEFSFSQVLKSQWGSDPLFLGSYSYVAVGSSGE 474

Query: 421 DLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNCI 468
           DLDAMAEPLPRTEE S+APLLQILFAGEATHRTHYSTTHGAY+SGLREANRLLHHYNCI
Sbjct: 475 DLDAMAEPLPRTEESSKAPLLQILFAGEATHRTHYSTTHGAYYSGLREANRLLHHYNCI 533

BLAST of Spg003561 vs. ExPASy TrEMBL
Match: A0A6J1G2I5 (probable polyamine oxidase 5 OS=Cucurbita moschata OX=3662 GN=LOC111450217 PE=4 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.2e-221
Identity = 384/481 (79.83%), Postives = 423/481 (87.94%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPI++IAD+I AL+SDQPWECMDGYS   T+VAEGGVEL PAT
Sbjct: 55  MGERIEMGATWIHGIGGSPIYEIADRIGALNSDQPWECMDGYSGQSTTVAEGGVELSPAT 114

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
            D ISTLFKNLMDFAQGKI+G+SN  R+ N D  S+G FLRRGL+ YW S+NG  EVNGC
Sbjct: 115 VDPISTLFKNLMDFAQGKIAGESNFLRRGNYDKLSVGAFLRRGLESYWLSKNGVTEVNGC 174

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
            EW++K+LEEAIFAM+EN QRTYTSAGELSTLDF++ESEYQMFPGEEITIAKGY SVI+S
Sbjct: 175 GEWSQKALEEAIFAMYENNQRTYTSAGELSTLDFVAESEYQMFPGEEITIAKGYLSVIES 234

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEW+PE + RNIPTPV LHF+DGS  SADHVIVTVSLGVLKA
Sbjct: 235 IASVLPPGLVQLGKKVTKIEWNPESESRNIPTPVTLHFADGSHTSADHVIVTVSLGVLKA 294

Query: 241 ATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFH 300
            T++DS +FNPPLPSFKTEAISRLGFGVVNKLFL+L+P   DG++ +S NKFP LNFVFH
Sbjct: 295 GTREDSALFNPPLPSFKTEAISRLGFGVVNKLFLRLSPPTNDGDNPKSINKFPCLNFVFH 354

Query: 301 RPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTIS 360
           +PDSEFRRK+IPWWMR+TT+L+PIYQNS ILLSWFAGEEAL LEKL D+EIINGVSTTIS
Sbjct: 355 QPDSEFRRKRIPWWMRKTTTLQPIYQNSSILLSWFAGEEALQLEKLKDDEIINGVSTTIS 414

Query: 361 NFLIP-----NSESD---WNNGERN------VKLTNVLKSQWGSDPLFLGSYSYVAVGSS 420
           NFLIP     NSESD   W+NG+RN         + VLKSQWGSDPLF GSYSYVAVGSS
Sbjct: 415 NFLIPRSQNENSESDSNGWSNGQRNQNDTVEFHFSQVLKSQWGSDPLFQGSYSYVAVGSS 474

Query: 421 GEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 468
           GEDLDAMAEPLPRTEE  +APLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC
Sbjct: 475 GEDLDAMAEPLPRTEESPKAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 534

BLAST of Spg003561 vs. ExPASy TrEMBL
Match: A0A0A0LFR4 (Amino_oxidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000460 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.7e-220
Identity = 386/480 (80.42%), Postives = 424/480 (88.33%), Query Frame = 0

Query: 1   MGERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPAT 60
           MGERIEMGATWIHGIGGSPI+KIA+QI ALHSDQ WECMDGYS   T+VAEGG+EL PAT
Sbjct: 55  MGERIEMGATWIHGIGGSPIYKIAEQIGALHSDQSWECMDGYSGQSTTVAEGGIELSPAT 114

Query: 61  FDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGC 120
            D ISTLF+ LMDFAQGKI+GDS+I +QAN D RSIG FL++G+D YW S+NGE EVNGC
Sbjct: 115 VDPISTLFQKLMDFAQGKITGDSDILQQANYDKRSIGEFLQQGIDSYWVSKNGETEVNGC 174

Query: 121 QEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQS 180
           +EW++KSLEEAIFAM+EN QRTYTSAG+LSTLDFISESEYQMFPGEEITIAKGY SVI+S
Sbjct: 175 KEWSQKSLEEAIFAMYENNQRTYTSAGDLSTLDFISESEYQMFPGEEITIAKGYLSVIES 234

Query: 181 IASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKA 240
           IASVLPPGLVQLGKKVTKIEWHPE D  NIPTPV LHF+DGS +SADHVIVTVSLGVLKA
Sbjct: 235 IASVLPPGLVQLGKKVTKIEWHPELDPPNIPTPVTLHFADGSHISADHVIVTVSLGVLKA 294

Query: 241 ATQQD--SGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFV 300
            TQ D  S +F+PPLPSFKTEAISRLGFGVVNKLFL+LAP   +G +L+  ++FP LNFV
Sbjct: 295 GTQPDSPSPLFHPPLPSFKTEAISRLGFGVVNKLFLRLAPVTENGLNLKRTHQFPCLNFV 354

Query: 301 FHRPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTT 360
           FH+PDS+FRRKKIPWWMR+TTSLRPIYQNS +LLSW AGEEALHLEKL D+EIINGVSTT
Sbjct: 355 FHQPDSKFRRKKIPWWMRKTTSLRPIYQNSSLLLSWLAGEEALHLEKLKDDEIINGVSTT 414

Query: 361 ISNFLI-----PNSESDWN-----NGERNVK--LTNVLKSQWGSDPLFLGSYSYVAVGSS 420
           ISNFLI      NSESD N     NG RN++   + VLKSQWGSDPLFLGSYSYVAVGSS
Sbjct: 415 ISNFLIQKSESENSESDSNCCNKMNGHRNIEFSFSQVLKSQWGSDPLFLGSYSYVAVGSS 474

Query: 421 GEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 467
           GEDLDAMAEPLPRTEE S++PLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC
Sbjct: 475 GEDLDAMAEPLPRTEESSKSPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLLHHYNC 534

BLAST of Spg003561 vs. TAIR 10
Match: AT4G29720.1 (polyamine oxidase 5 )

HSP 1 Score: 543.9 bits (1400), Expect = 1.3e-154
Identity = 286/497 (57.55%), Postives = 347/497 (69.82%), Query Frame = 0

Query: 3   ERIEMGATWIHGIGGSPIHKIADQINALHSDQPWECMDGYSDHPTSVAEGGVELPPATFD 62
           E+IEMGATWIHGIGGSP+++IA +  +L SD+PWECMD   D   + AEGG E+ P+  +
Sbjct: 54  EKIEMGATWIHGIGGSPVYRIAKETGSLVSDEPWECMDSTIDKAKTFAEGGFEIEPSIVE 113

Query: 63  SISTLFKNLMDFAQGK--ISGDSNIPRQAN-----------SDNRSIGGFLRRGLDLYWQ 122
           SIS LF  LM+ AQGK     D+++ R A+             + S+G FL+ G D YW 
Sbjct: 114 SISGLFTALMELAQGKEISQSDADLSRLAHIYETATRVCSKGSSTSVGSFLKSGFDAYWD 173

Query: 123 --SRNGEIEVNGCQEWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEE 182
             S  GE  V G  +W++KSLEEAIF MF NTQRTYTSA ELSTLDF +ESEYQMFPGEE
Sbjct: 174 SISNGGEEGVKGYGKWSRKSLEEAIFTMFSNTQRTYTSADELSTLDFAAESEYQMFPGEE 233

Query: 183 ITIAKGYFSVIQSIASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSAD 242
           ITIAKGY SVI  +ASVLP G++QL +KVTKIEW            V+LHFSDGS + AD
Sbjct: 234 ITIAKGYLSVIHHLASVLPQGVIQLNRKVTKIEWQ--------SNEVKLHFSDGSVVFAD 293

Query: 243 HVIVTVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLE 302
           HVIVTVSLGVLKA  + D+ +F+PPLP FK++AI RLG+GVVNKLF+++           
Sbjct: 294 HVIVTVSLGVLKAGIETDAELFSPPLPDFKSDAIRRLGYGVVNKLFVEM----------- 353

Query: 303 SRNKFPGLNFVFHRPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLN 362
           S+ KFP L  VF R DSEFR  KIPWWMRRT ++ PI+ NS +LLSWFAG+EAL LEKL 
Sbjct: 354 SQRKFPSLQLVFDREDSEFRFVKIPWWMRRTATITPIHSNSKVLLSWFAGKEALELEKLT 413

Query: 363 DEEIINGVSTTIS---------NFLIPNSESDWNNGERNVKLTNVLKSQWGSDPLFLGSY 422
           DEEI + V TTIS         +   P +    N+ +  +K+T VLKS+WGSDPLF GSY
Sbjct: 414 DEEIKDAVMTTISCLTGKEVKNDTAKPLTNGSLNDDDEAMKITKVLKSKWGSDPLFRGSY 473

Query: 423 SYVAVGSSGEDLDAMAEPLPRT---------EEGSEAPLLQILFAGEATHRTHYSTTHGA 467
           SYVAVGSSG+DLDAMAEPLP+           + ++   LQ++FAGEATHRTHYSTTHGA
Sbjct: 474 SYVAVGSSGDDLDAMAEPLPKINKKVGQVNGHDQAKVHELQVMFAGEATHRTHYSTTHGA 531

BLAST of Spg003561 vs. TAIR 10
Match: AT3G13682.1 (LSD1-like2 )

HSP 1 Score: 121.3 bits (303), Expect = 2.0e-27
Identity = 110/330 (33.33%), Postives = 162/330 (49.09%), Query Frame = 0

Query: 143 YTSAGELSTLD---FISESEYQMFPGEEITIAKGYFSVIQSIASVLPPGLVQLGKKVTKI 202
           Y +AG LS L    +  +  Y+M  G+   +A G + +I ++A  LP   +  GK V  I
Sbjct: 322 YANAGCLSNLSAAYWDQDDPYEM-GGDHCFLAGGNWRLINALAEGLP---IIYGKSVDTI 381

Query: 203 EWHPEPDGRNIPTPVRLHFSDGSDM-SADHVIVTVSLGVLKAATQQDSGMFNPPLPSFKT 262
           ++    DG        +    GS +  AD ++ TV LGVLK    + S  F P LP  K 
Sbjct: 382 KY---GDG-------GVEVISGSQIFQADMILCTVPLGVLK----KRSIKFEPELPRRKQ 441

Query: 263 EAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFHRPDSEFRRKKIPWWMRRT 322
            AI RLGFG++NK+ + L P+   G++L++   F  LN       S  R +   ++   T
Sbjct: 442 AAIDRLGFGLLNKVAM-LFPSVFWGDELDT---FGCLN-----ESSINRGEFFLFYAYHT 501

Query: 323 TSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTISNFLIPNSESDWNNGERNV 382
            S        P L++  AGE A   E      +++ V   +              G + V
Sbjct: 502 VS------GGPALVALVAGEAAQRFECTEPSVLLHRVLKKLRGIY----------GPKGV 561

Query: 383 KLTNVLK---SQWGSDPLFLGSYSYVAVGSSGEDLDAMAEPLPRTEEGSEAPLLQILFAG 442
            + + ++   ++WGSDPL  GSYS+V VGSSG D D +AE +            ++ FAG
Sbjct: 562 VVPDPIQTVCTRWGSDPLSYGSYSHVRVGSSGVDYDILAESVSN----------RLFFAG 598

Query: 443 EATHRTHYSTTHGAYFSGLREANRLLHHYN 466
           EAT R H +T HGAY SGLREA+++LH  N
Sbjct: 622 EATTRQHPATMHGAYLSGLREASKILHVAN 598

BLAST of Spg003561 vs. TAIR 10
Match: AT4G16310.1 (LSD1-like 3 )

HSP 1 Score: 117.9 bits (294), Expect = 2.2e-26
Identity = 92/319 (28.84%), Postives = 148/319 (46.39%), Query Frame = 0

Query: 148  ELSTLDFISESEYQMFPGEEITIAKGYFSVIQSIASVLPPGLVQLGKKVTKIEWHPEPDG 207
            E+S   +  +  Y  F G    I  GY  V++S+A  L    + L K V+ + +  +   
Sbjct: 829  EVSLPHWNQDEFYGGFGGPHAMIKGGYSRVVESLAEGLD---IHLNKIVSDVSYVSDVSA 888

Query: 208  R-NIPTPVRLHFSDGSDMSADHVIVTVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLGF 267
              N    VR+  S+G +   D V+VTV LG LKA T +    F+PPLP +K  +I +LGF
Sbjct: 889  MDNSKHKVRVSTSNGCEYLGDAVLVTVPLGCLKAETIK----FSPPLPDWKYASIKQLGF 948

Query: 268  GVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFHRPDSEFRRKKIPWWMRRTTSLRPIYQ 327
            GV+NK+ L+      D    +S + F          +++ R +   +W  + T       
Sbjct: 949  GVLNKVVLEFPTVFWD----DSVDYFGAT-----AEETDLRGECFMFWNVKKT------V 1008

Query: 328  NSPILLSWFAGEEALHLEKLNDEEIINGVSTTISNF----LIPNSESDWNNGERNVKLTN 387
             +P+L++   G+ A      +  E +N     +       L+P+  +             
Sbjct: 1009 GAPVLIALVVGKAAFEYTNKSKSEHVNHAMMVLRKLFGGDLVPDPVAS------------ 1068

Query: 388  VLKSQWGSDPLFLGSYSYVAVGSSGEDLDAMAEPLPRTEEGSEAPLLQILFAGEATHRTH 447
             + + WG+DP   G+YSYVA+G+SGED D +  P+             + FAGEAT + H
Sbjct: 1069 -VVTDWGTDPYSYGAYSYVAIGASGEDYDVLGRPVQNC----------LFFAGEATCKEH 1102

Query: 448  YSTTHGAYFSGLREANRLL 462
              T  GA  +G+REA R++
Sbjct: 1129 PDTVGGAMMTGVREAVRII 1102

BLAST of Spg003561 vs. TAIR 10
Match: AT1G62830.1 (LSD1-like 1 )

HSP 1 Score: 111.7 bits (278), Expect = 1.6e-24
Identity = 124/458 (27.07%), Postives = 198/458 (43.23%), Query Frame = 0

Query: 6   EMGATWIHGIGGSPIHKIADQIN-ALHSDQPWECMDGYSDHPTSVAEGGVELPPATFDS- 65
           ++G + + GI G+P+  +A Q+   LH  +           P  +  G  EL  A+ DS 
Sbjct: 320 DVGGSVLTGINGNPLGVLARQLGLPLHKVR--------DICPLYLPNG--ELADASVDSK 379

Query: 66  ISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNGEIEVNGCQEW 125
           I   F  L+D           + +    +N+S+   L   L+ +       +     ++ 
Sbjct: 380 IEASFNKLLDRV-------CKLRQSMIEENKSVDVPLGEALETF------RLVYGVAEDQ 439

Query: 126 TKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEITIAKGYFSVIQSIAS 185
            ++ L +   A  E    T    G LS   +  +  Y+M  G+   I  G    + ++A 
Sbjct: 440 QERMLLDWHLANLEYANATL--LGNLSMAYWDQDDPYEM-GGDHCFIPGGNEIFVHALAE 499

Query: 186 VLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHVIVTVSLGVLKAATQ 245
            LP   +  G  V  I +     G N      L ++   +   D  + TV LGVLK  + 
Sbjct: 500 NLP---IFYGSTVESIRY-----GSN----GVLVYTGNKEFHCDMALCTVPLGVLKKGSI 559

Query: 246 QDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESRNKFPGLNFVFHRPD 305
           +    F P LP  K EAI RLGFG++NK+ + L P    GE++++  +           D
Sbjct: 560 E----FYPELPHKKKEAIQRLGFGLLNKVAM-LFPCNFWGEEIDTFGRL--------TED 619

Query: 306 SEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDEEIINGVSTTISNFL 365
              R +   ++   + S        P+L++  AG+ A   E L+  + +  V   +    
Sbjct: 620 PSTRGEFFLFYSYSSVS------GGPLLVALVAGDAAERFETLSPTDSVKRVLQILRGIY 679

Query: 366 IPNSESDWNNGERNVKLTNVLKSQWGSDPLFLGSYSYVAVGSSGEDLDAMAEPLPRTEEG 425
            P        G         L S+WG D    GSYSYVAVGSSG+D D +AE +    +G
Sbjct: 680 HP-------KGIVVPDPVQALCSRWGQDKFSYGSYSYVAVGSSGDDYDILAESV---GDG 704

Query: 426 SEAPLLQILFAGEATHRTHYSTTHGAYFSGLREANRLL 462
                 ++ FAGEAT+R + +T HGA+ SG+REA  +L
Sbjct: 740 ------RVFFAGEATNRQYPATMHGAFLSGMREAANIL 704

BLAST of Spg003561 vs. TAIR 10
Match: AT1G65840.1 (polyamine oxidase 4 )

HSP 1 Score: 96.7 bits (239), Expect = 5.4e-20
Identity = 121/465 (26.02%), Postives = 192/465 (41.29%), Query Frame = 0

Query: 5   IEMGATWIHGIGG-SPIHKIADQI----------NALHSDQPWECMDGYSDHPTSVAEGG 64
           ++MGA+W+HG+   +P+  I  ++          +++  D   E    +  H       G
Sbjct: 77  VDMGASWLHGVSDENPLAPIIRRLGLTLYRTSGDDSILYDHDLESYGLFDMH-------G 136

Query: 65  VELPPATFDSISTLFKNLMDFAQGKISGDSNIPRQANSDNRSIGGFLRRGLDLYWQSRNG 124
            ++PP     +   FK +++        ++   R   +++ S+   +   LD     RN 
Sbjct: 137 NKIPPQLVTKVGDAFKRILE--------ETEKIRDETANDMSVLQGISIVLD-----RNP 196

Query: 125 EIEVNGCQ----EWTKKSLEEAIFAMFENTQRTYTSAGELSTLDFISESEYQMFPGEEIT 184
           E+   G      +W    + EA FA+  N          L +L    + E     G    
Sbjct: 197 ELRQEGMAYEVLQWYLCRM-EAWFAVDAN----------LISLKCWDQDE--CLSGGHGL 256

Query: 185 IAKGYFSVIQSIASVLPPGLVQLGKKVTKIEWHPEPDGRNIPTPVRLHFSDGSDMSADHV 244
           + +GY  VI++IA  L    ++L  +VTK+        R     V +    G++  AD V
Sbjct: 257 MVQGYEPVIRTIAKDLD---IRLNHRVTKVV-------RTSNNKVIVAVEGGTNFVADAV 316

Query: 245 IVTVSLGVLKAATQQDSGMFNPPLPSFKTEAISRLGFGVVNKLFLQLAPAAADGEDLESR 304
           I+TV +GVLKA   Q    F P LP +KT AIS LG G  NK+ L+             R
Sbjct: 317 IITVPIGVLKANLIQ----FEPELPQWKTSAISGLGVGNENKIALRF-----------DR 376

Query: 305 NKFPGLNFVFHRPDSEFRRKKIPWWMRRTTSLRPIYQNSPILLSWFAGEEALHLEKLNDE 364
             +P + F+     + +         + T          P+L+   AG  A  LEKL+DE
Sbjct: 377 AFWPNVEFLGMVAPTSYACGYFLNLHKAT--------GHPVLVYMAAGNLAQDLEKLSDE 436

Query: 365 EIINGVSTTISNFLIPNSESDWNNGERNVKLTNVLKSQWGSDPLFLGSYSYVAVGSSGED 424
              N V   +   + P++                L ++WG+DP  LG Y+Y  VG   ED
Sbjct: 437 ATANFVMLQLKK-MFPDAPDP----------AQYLVTRWGTDPNTLGCYAYDVVGMP-ED 453

Query: 425 LDAMAEPLPRTEEGSEAPLLQILFAGEATHRTHYSTTHGAYFSGL 455
           L       PR  E    P+  I F GEA +  H  + HGA+ +G+
Sbjct: 497 L------YPRLGE----PVDNIFFGGEAVNVEHQGSAHGAFLAGV 453

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022999455.12.1e-22380.87probable polyamine oxidase 5 [Cucurbita maxima][more]
XP_008459345.12.3e-22281.42PREDICTED: probable polyamine oxidase 5 [Cucumis melo] >KAA0061493.1 putative po... [more]
XP_038889398.11.1e-22179.71probable polyamine oxidase 5 [Benincasa hispida][more]
KAG6599509.11.5e-22180.04putative polyamine oxidase 5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022945998.12.5e-22179.83probable polyamine oxidase 5 [Cucurbita moschata] >XP_022945999.1 probable polya... [more]
Match NameE-valueIdentityDescription
Q9SU791.8e-15357.55Probable polyamine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PAO5 PE=1 SV=1[more]
Q5NAI71.1e-10244.54Polyamine oxidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAO1 PE=1 SV=1[more]
Q99K823.6e-5332.54Spermine oxidase OS=Mus musculus OX=10090 GN=Smox PE=1 SV=1[more]
Q9NWM07.5e-5132.28Spermine oxidase OS=Homo sapiens OX=9606 GN=SMOX PE=1 SV=1[more]
Q8C0L61.4e-4932.14Peroxisomal N(1)-acetyl-spermine/spermidine oxidase OS=Mus musculus OX=10090 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1KFF81.0e-22380.87probable polyamine oxidase 5 OS=Cucurbita maxima OX=3661 GN=LOC111493815 PE=4 SV... [more]
A0A5A7UZX31.1e-22281.42Putative polyamine oxidase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CB611.1e-22281.42probable polyamine oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103498503 PE=4 SV=1[more]
A0A6J1G2I51.2e-22179.83probable polyamine oxidase 5 OS=Cucurbita moschata OX=3662 GN=LOC111450217 PE=4 ... [more]
A0A0A0LFR46.7e-22080.42Amino_oxidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G0004... [more]
Match NameE-valueIdentityDescription
AT4G29720.11.3e-15457.55polyamine oxidase 5 [more]
AT3G13682.12.0e-2733.33LSD1-like2 [more]
AT4G16310.12.2e-2628.84LSD1-like 3 [more]
AT1G62830.11.6e-2427.07LSD1-like 1 [more]
AT1G65840.15.4e-2026.02polyamine oxidase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.660.10coord: 38..406
e-value: 1.5E-67
score: 231.1
NoneNo IPR availablePANTHERPTHR10742:SF374POLYAMINE OXIDASE 5-RELATEDcoord: 2..467
NoneNo IPR availablePANTHERPTHR10742FLAVIN MONOAMINE OXIDASEcoord: 2..467
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 265..400
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 2..459
e-value: 1.5E-67
score: 231.1
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 2..464
IPR002937Amine oxidasePFAMPF01593Amino_oxidasecoord: 2..461
e-value: 1.7E-71
score: 241.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003561.1Spg003561.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048510 regulation of timing of transition from vegetative to reproductive phase
biological_process GO:0046208 spermine catabolic process
biological_process GO:1903602 thermospermine catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0052903 N1-acetylspermine:oxygen oxidoreductase (3-acetamidopropanal-forming) activity
molecular_function GO:0052894 norspermine:oxygen oxidoreductase activity
molecular_function GO:0046592 polyamine oxidase activity
molecular_function GO:0052901 spermine:oxygen oxidoreductase (spermidine-forming) activity
molecular_function GO:1990534 thermospermine oxidase activity
molecular_function GO:0016491 oxidoreductase activity