Spg003558 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003558
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	acidic mammalian chitinase-like
Location	scaffold4: 47136600 .. 47137667 (-)
RNA-Seq Expression	Spg003558
Synteny	Spg003558

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAAGCTGTGAGGGGTGGTTATTGGTTGGCGAGCACCAACGCCATACTTTCTCCTGGAATGATCGATGCTAAGCTTTTCAGCCATCTGTTTGTTGGTTACGCTGGTGTTGACGAGAACACTTATGAAGTGACCATAGAGTCCTCAGAAAGTATCCTAATCAATCAGTTTACTCCAACGGTGAAAGGAAAAAATGGTGAGGTGAAAACTATTCTGTCCATTGCAGGCAGAAGTGATGTATTCGCTAAAATTGCTGAGCAGCCTACAAATCGAGATGTCTTCATCACATCCTCCATTGCTGCGGCCAGGGCTGGGCAGTTTGATGGCCTGGATCTTCAGTGGTTATACCCATCTTCTGCAGATGAAATGAACAACTTCAGATCTCTTATTAGTTCTTGGAAAGAAGGTGTTACCAGAGAAGCTAGGAGTTCTAACCGTCCACCCTTACTCCTTGTTGCCACAGTCTCCAACTTACCATATCTTCAAAACAACCTTCAGTATCCAGTTGCTGCAATAAAAGTAAATCTGAATTGGGTGAACCTGGTTTCTTATGACTTTTACACCCCAACTTCCTCGAGGATTTCTACTGGACCATCCTCAGCATTCCAAAATTCAAATGCACCACAAAATTCTGCGCTTTTCGGAATTAAGGATTGGATGACCAAAGGCCTTACCCATCTGCCTCGTAAGCAGATTGTATTTGGAATTCCTTTCCACGGCTGGGCTTGGAAACTTTTCAACGCCAACCAACACCAAGTGTTTTCTAGAGCCGATGGAGCAGCTAGAGGAGAGAGTAGTTATCAAATCTCACAAGATGGCAAAATCTACTACTCCGATGTGAAAAAGTTCATAGCCACTAAAAATGCACCGGAAGAGACGGACGCCGCATATCAAATTGCCTACACTTATTCTGGTCCAACCTGGATTGTGTACGAAACTGGAGACACCATTGCCAAAAAGATTCTTTCCGCAAAGTCTGAAAACGTGCTTGGTTACTTTGCCTTCAACATTGCAGCCGATGATGATGTTTTCACTCTTGCCAGTGCTGCCAACGCCAGTTGGTGA

mRNA sequence

ATGGCAGAAGCTGTGAGGGGTGGTTATTGGTTGGCGAGCACCAACGCCATACTTTCTCCTGGAATGATCGATGCTAAGCTTTTCAGCCATCTGTTTGTTGGTTACGCTGGTGTTGACGAGAACACTTATGAAGTGACCATAGAGTCCTCAGAAAGTATCCTAATCAATCAGTTTACTCCAACGGTGAAAGGAAAAAATGGTGAGGTGAAAACTATTCTGTCCATTGCAGGCAGAAGTGATGTATTCGCTAAAATTGCTGAGCAGCCTACAAATCGAGATGTCTTCATCACATCCTCCATTGCTGCGGCCAGGGCTGGGCAGTTTGATGGCCTGGATCTTCAGTGGTTATACCCATCTTCTGCAGATGAAATGAACAACTTCAGATCTCTTATTAGTTCTTGGAAAGAAGGTGTTACCAGAGAAGCTAGGAGTTCTAACCGTCCACCCTTACTCCTTGTTGCCACAGTCTCCAACTTACCATATCTTCAAAACAACCTTCAGTATCCAGTTGCTGCAATAAAAGTAAATCTGAATTGGGTGAACCTGGTTTCTTATGACTTTTACACCCCAACTTCCTCGAGGATTTCTACTGGACCATCCTCAGCATTCCAAAATTCAAATGCACCACAAAATTCTGCGCTTTTCGGAATTAAGGATTGGATGACCAAAGGCCTTACCCATCTGCCTCGTAAGCAGATTGTATTTGGAATTCCTTTCCACGGCTGGGCTTGGAAACTTTTCAACGCCAACCAACACCAAGTGTTTTCTAGAGCCGATGGAGCAGCTAGAGGAGAGAGTAGTTATCAAATCTCACAAGATGGCAAAATCTACTACTCCGATGTGAAAAAGTTCATAGCCACTAAAAATGCACCGGAAGAGACGGACGCCGCATATCAAATTGCCTACACTTATTCTGGTCCAACCTGGATTGTGTACGAAACTGGAGACACCATTGCCAAAAAGATTCTTTCCGCAAAGTCTGAAAACGTGCTTGGTTACTTTGCCTTCAACATTGCAGCCGATGATGATGTTTTCACTCTTGCCAGTGCTGCCAACGCCAGTTGGTGA

Coding sequence (CDS)

ATGGCAGAAGCTGTGAGGGGTGGTTATTGGTTGGCGAGCACCAACGCCATACTTTCTCCTGGAATGATCGATGCTAAGCTTTTCAGCCATCTGTTTGTTGGTTACGCTGGTGTTGACGAGAACACTTATGAAGTGACCATAGAGTCCTCAGAAAGTATCCTAATCAATCAGTTTACTCCAACGGTGAAAGGAAAAAATGGTGAGGTGAAAACTATTCTGTCCATTGCAGGCAGAAGTGATGTATTCGCTAAAATTGCTGAGCAGCCTACAAATCGAGATGTCTTCATCACATCCTCCATTGCTGCGGCCAGGGCTGGGCAGTTTGATGGCCTGGATCTTCAGTGGTTATACCCATCTTCTGCAGATGAAATGAACAACTTCAGATCTCTTATTAGTTCTTGGAAAGAAGGTGTTACCAGAGAAGCTAGGAGTTCTAACCGTCCACCCTTACTCCTTGTTGCCACAGTCTCCAACTTACCATATCTTCAAAACAACCTTCAGTATCCAGTTGCTGCAATAAAAGTAAATCTGAATTGGGTGAACCTGGTTTCTTATGACTTTTACACCCCAACTTCCTCGAGGATTTCTACTGGACCATCCTCAGCATTCCAAAATTCAAATGCACCACAAAATTCTGCGCTTTTCGGAATTAAGGATTGGATGACCAAAGGCCTTACCCATCTGCCTCGTAAGCAGATTGTATTTGGAATTCCTTTCCACGGCTGGGCTTGGAAACTTTTCAACGCCAACCAACACCAAGTGTTTTCTAGAGCCGATGGAGCAGCTAGAGGAGAGAGTAGTTATCAAATCTCACAAGATGGCAAAATCTACTACTCCGATGTGAAAAAGTTCATAGCCACTAAAAATGCACCGGAAGAGACGGACGCCGCATATCAAATTGCCTACACTTATTCTGGTCCAACCTGGATTGTGTACGAAACTGGAGACACCATTGCCAAAAAGATTCTTTCCGCAAAGTCTGAAAACGTGCTTGGTTACTTTGCCTTCAACATTGCAGCCGATGATGATGTTTTCACTCTTGCCAGTGCTGCCAACGCCAGTTGGTGA

Protein sequence

MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWVNLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETDAAYQIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW

Homology

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BLAST of Spg003558 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.7 bits (1075), Expect = 5.0e-113
Identity = 215/356 (60.39%), Postives = 264/356 (74.16%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           + E V  GYWLAST+  L    IDA LF+HLF GYA ++ +TY+VTI+ S+ IL+NQF+P
Sbjct: 13  VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSP 72

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TV+  N +VKT+LSIA  + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 73  TVRLSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 132

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
            +EM NF++LI SW + V  +A S + P LLLVATVSNLPYLQ+N+QYP  AI   L+WV
Sbjct: 133 QEEMGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 192

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP S+R +TGPSSAF N  A   SA FGI+ W  + L +LP K+IVFGIPFH
Sbjct: 193 NLLSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLPHKKIVFGIPFH 252

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKLFN++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 253 GWAWKLFNSHQHTLFAKADGAATGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 312

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AYTY   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 313 VAYTYFETTWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 364

[Close]

BLAST of Spg003558 vs. NCBI nr
Match: XP_023545402.1 (acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.8 bits (1070), Expect = 1.9e-112
Identity = 214/352 (60.80%), Postives = 262/352 (74.43%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           V  GYWLAST+  L    IDA LF+HLF GYA ++ +TY+VTI+ S+ IL+NQF+PTV+ 
Sbjct: 2   VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVRL 61

Query: 65  KNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSADEM 124
            N +VKT+LSIA  + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS +EM
Sbjct: 62  SNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEEM 121

Query: 125 NNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWVNLVS 184
            NF++LI SW + V  +A S + P LLLVATVSNLPYLQ+N+QYP  AI   L+WVNL+S
Sbjct: 122 GNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 181

Query: 185 YDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFHGWAW 244
           YDFYTP S+R +TGPSSAF N  A   SA FGI+ W  + L +LP K+IVFGIPFHGWAW
Sbjct: 182 YDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLPHKKIVFGIPFHGWAW 241

Query: 245 KLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQIAYT 304
           KLFN++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y +AYT
Sbjct: 242 KLFNSHQHTLFAKADGAATGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYT 301

Query: 305 YSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           Y   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 302 YFETTWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 349

[Close]

BLAST of Spg003558 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 415.6 bits (1067), Expect = 4.3e-112
Identity = 215/356 (60.39%), Postives = 262/356 (73.60%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           + E V  GYWLAST+  L    IDA LF+HLF GYA ++  TY+VT++ S+ ILI+QF+ 
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSS 66

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TVK  N EVKT+LSIA  + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
            D+M+NF++LI SW + V  +A S + P LLLVATVSNLPYLQ+N+QYP  AI   L+WV
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 186

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP SSR +TGPSSAF N  A   SA FGI+ W  + L +LP K+IVFGIPFH
Sbjct: 187 NLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLPHKKIVFGIPFH 246

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKLFN++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 247 GWAWKLFNSHQHTLFAKADGAATGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 306

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AY Y   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 307 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Spg003558 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 415.2 bits (1066), Expect = 5.6e-112
Identity = 215/356 (60.39%), Postives = 263/356 (73.88%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           + E V  GYWLAST+  L    IDA LF+HLF GYA ++ +TY+VTI+ S+ ILI+QF+ 
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILIDQFSS 66

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TVK  N EVKT+LSIA  + +FA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
            D+M+NF++LI SW + V  +A S + P LLLVATVSNLPYLQ+N+QYP  AI   L+WV
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 186

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP SSR +TGPSSAF N  A   SA FGI+ W  + L +LP K+IVFGIPFH
Sbjct: 187 NLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLPHKKIVFGIPFH 246

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKLFN++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 247 GWAWKLFNSHQHTLFAKADGAATGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 306

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AY Y   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 307 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Spg003558 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414.5 bits (1064), Expect = 9.5e-112
Identity = 215/356 (60.39%), Postives = 263/356 (73.88%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           + E V  GYWLAST+  L    IDA LF+HLF GYA ++ +TY+VTI+ S  ILI+QF+ 
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSS 66

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TVK  N EVKT+LSIA  + +FA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
            D+M+NF++LI SW + V  +A S + P LLLVATVSNLPYLQ+N+QYP  AI   L+WV
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 186

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP SSR +TGPSSAF N  A   SA FGI+ W+ + L +LP K+IVFGIPFH
Sbjct: 187 NLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWV-QSLHNLPHKKIVFGIPFH 246

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKLFN++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 247 GWAWKLFNSHQHTLFAKADGAATGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 306

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AY Y   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 307 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Spg003558 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.9e-65
Identity = 144/357 (40.34%), Postives = 207/357 (57.98%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           V+  YW  ++   ++   ID+ LF+HLF  +A ++  T +VT+ S+     + FT TV+ 
Sbjct: 28  VKASYWFPASEFPVTD--IDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 65  KNGEVKTILSIAG---RSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSA 124
           +N  VKT+LSI G       +A +A  PT+R  FI SSI  AR+  F GLDL W YPSSA
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 125 DEMNNFRSLISSWKEGVTREARSSNRPPLLLVATV--SNLPYLQNNLQYPVAAIKVNLNW 184
            EM NF +L+  W+  V  EA SS +P LLL A V  SN  Y   ++ YPV+A+  +L+W
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 185 VNLVSYDFYTPTSSRISTGPSSAFQNSNA-PQNSALFGIKDWMTKGLTHLPRKQIVFGIP 244
           VNL++YDFY P  SR++  P++ F  SNA P   A  G + W+  G   LP K+ V G P
Sbjct: 208 VNLMAYDFYGPGWSRVTGPPAALFDPSNAGPSGDA--GTRSWIQAG---LPAKKAVLGFP 267

Query: 245 FHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETDAAY 304
           ++G+AW+L NAN H  ++   GAA       IS DG I Y  ++KFI    A    ++  
Sbjct: 268 YYGYAWRLTNANSHSYYAPTTGAA-------ISPDGSIGYGQIRKFIVDNGATTVYNSTV 327

Query: 305 QIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
              Y Y+G  WI Y+   +I  K+  AK   +LGYF++++ ADD+   L+ AA+ +W
Sbjct: 328 VGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

[Close]

BLAST of Spg003558 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 8.5e-47
Identity = 120/358 (33.52%), Postives = 186/358 (51.96%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           V+G YWL   N    P  +D  LF+H+F  +   +  TY++ I SS+ + +N FT T K 
Sbjct: 35  VKGIYWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKT 94

Query: 65  KNGEVKTILSIAG---RSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSA 124
           K+    T+ SI G    S + A IA  P     FI S+I  AR   FDG+DL W +P + 
Sbjct: 95  KSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNT 154

Query: 125 DEMNNFRSLISSWKEGVTREARSSNRPPLLLVATV---SNLPYLQNNLQYPVAAIKVNLN 184
            EMN+   ++  W++ ++ E  ++ RPPLLL A V    N         YPV +I  NL+
Sbjct: 155 KEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLD 214

Query: 185 WVNLVSYDFYTPTSSRISTGPSSAFQ-NSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGI 244
           WVN++SY+   P S++ +  PS  F   SN    S + G+  W+  G+  +P K +V G+
Sbjct: 215 WVNVMSYELRGPRSNK-TGAPSGTFDPKSNV---SVVSGLLSWIHSGV--VPEK-LVMGM 274

Query: 245 PFHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETDAA 304
           P +G +WKL + N H + + + G+  G        +G + Y  V  F   K+A  E D  
Sbjct: 275 PLYGKSWKLRDPNVHGIGAPSVGSGPG-------VNGLMAYFQVLDFNRQKSAKVEYDVD 334

Query: 305 YQIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
               Y+YSG TWI Y+   T++ K+  A++  + GYF +    D   + +A+ A+ +W
Sbjct: 335 TASVYSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKAW 376

[Close]

BLAST of Spg003558 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 182.6 bits (462), Expect = 7.9e-45
Identity = 120/359 (33.43%), Postives = 181/359 (50.42%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILI----NQFTP 64
           V+G YW+   N +  P  ID  LF+H+F  +   ++ TY++  E  +S  +      FT 
Sbjct: 37  VKGIYWI--ENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTTFTN 96

Query: 65  TVKGKNGEVKTILSIAG---RSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLY 124
           T K K   + T+LSI G    S +FA IA  PT R  FI S+I  AR   FDG+D  W +
Sbjct: 97  TFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDWEF 156

Query: 125 PSSADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATV---SNLPYLQNNLQYPVAAIK 184
           P++  EMN+   L+  W+  ++ E  S++RPPLLL A V    N         YPV +I 
Sbjct: 157 PTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDSIN 216

Query: 185 VNLNWVNLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIV 244
            NL+WVN++SYD    + S ++  PS  F + +    S + G+  W+  G+   P K IV
Sbjct: 217 KNLDWVNVMSYDL-RGSGSNVTGAPSGMFDSKS--NVSVVSGLFSWIRGGVA--PEK-IV 276

Query: 245 FGIPFHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEET 304
            G+P +G +WKL + N H + +   G   G        DG + Y  V  F     A    
Sbjct: 277 MGMPLYGKSWKLQDPNVHGIGAPNVGPGPG-------VDGGMAYFQVVDFNKQMGAKVVY 336

Query: 305 DAAYQIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANA 354
           D      Y+YSG TWI Y+   T++ K+  A++  + GYF +    D   + +++A+ A
Sbjct: 337 DKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFWAAGYDTSDWKVSTASKA 380

[Close]

BLAST of Spg003558 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 8.5e-39
Identity = 114/361 (31.58%), Postives = 184/361 (50.97%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           VR  YW A ++   S   ID   F+H++  YA V        +E +ES    ++ P   G
Sbjct: 55  VRAAYWPAWSD--FSTSSIDTNYFTHIY--YAFVQPAPESFNLEITES--YKKWAPKYDG 114

Query: 65  KNG---EVKTILSIAG---RSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYP 124
            +     V T+LSI G    + +F+++A    NR  FI S+I  AR  +F+GLDL W +P
Sbjct: 115 IHNIRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWP 174

Query: 125 SSADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATV---SNLPYLQNN-LQYPVAAIK 184
               +M+N   L+  W + +  EA +S +  LLL + V   S +  + N    YPV AI+
Sbjct: 175 GDEKDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIR 234

Query: 185 VNLNWVNLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIV 244
             L+W + + +D+    ++   TG ++A  + N+   S  +GI  W+  G   +P +++V
Sbjct: 235 KYLDWASPMCFDYNGAWAN--ETGFNAALYDPNS-NISTKYGIGSWIGSG---VPAEKLV 294

Query: 245 FGIPFHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEET 304
            G+P +G AW+L + N H V ++A G A       +  DG + Y ++  F     A    
Sbjct: 295 MGLPLYGRAWELKDPNDHGVGAKAVGPA-------VDTDGSMDYDEILVFNKDTGAKVVY 354

Query: 305 DAAYQIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANAS 356
           D      Y+YSG TWI Y+ G +I KK+  A+S  + GYF + I  D D +T++  A+ +
Sbjct: 355 DEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKD-WTISKQASNA 395

[Close]

BLAST of Spg003558 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.1e-37
Identity = 107/359 (29.81%), Postives = 179/359 (49.86%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           VR  YW A  +   SP +ID   F+H+ + +   +  ++++ I  S       F   ++ 
Sbjct: 40  VRSAYWPAGDD--FSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 65  KNGEVKTILSIAG---RSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSA 124
           ++  VKT+LSI G    S +F++IA    NR++FI S+I  AR  +FDG+DL W +P + 
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 125 DEMNNFRSLISSWKEGVTREARSSNRPPLLLVATV-----SNLPYLQNNLQYPVAAIKVN 184
            +M N   L   W   +  EA+   +P LLL + V       L        YP  AI   
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 185 LNWVNLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFG 244
           L+W + + +D++    +  +T  ++A  +S + + S  FG+  W+  G+   P K +V G
Sbjct: 220 LDWASPMCFDYHGTWDN--NTDFNAALYDSKS-EISTNFGLHSWIKSGVR--PEK-LVMG 279

Query: 245 IPFHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETDA 304
           +  +G AW+L + N + V + A G A          DG + Y+++ KF     A    D 
Sbjct: 280 LALYGRAWELKDPNVNGVGAEAVGPA-------TDTDGSMNYNEILKFNKQSGANVVYDK 339

Query: 305 AYQIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
                Y+Y+G TWI Y+ G +I  K+  AKS  + GYF + +  D D ++++  A+ +W
Sbjct: 340 VAISFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKD-WSISKQASNAW 382

[Close]

BLAST of Spg003558 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 2.1e-112
Identity = 215/356 (60.39%), Postives = 262/356 (73.60%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           + E V  GYWLAST+  L    IDA LF+HLF GYA ++  TY+VT++ S+ ILI+QF+ 
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSS 66

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TVK  N EVKT+LSIA  + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
            D+M+NF++LI SW + V  +A S + P LLLVATVSNLPYLQ+N+QYP  AI   L+WV
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 186

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP SSR +TGPSSAF N  A   SA FGI+ W  + L +LP K+IVFGIPFH
Sbjct: 187 NLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLPHKKIVFGIPFH 246

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKLFN++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 247 GWAWKLFNSHQHTLFAKADGAATGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 306

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AY Y   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 307 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Spg003558 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.5e-110
Identity = 212/356 (59.55%), Postives = 260/356 (73.03%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           + E V  GYWLAST+  L    IDA LF+HLF GYA ++ +TY+VT++ S+ ILI+QF+ 
Sbjct: 7   VTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSS 66

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TVK  N  VKT+LSIAG + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
            D+M NF++LI SW + V  +A S + P LLLVATVSNLPYLQNN+QYP  AI  NL+WV
Sbjct: 127 QDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLDWV 186

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP S R +TGPSSAF N  A   SA FGI+ W  + L +L  K+IVFGIPFH
Sbjct: 187 NLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSW-AQSLNNLSHKKIVFGIPFH 246

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKL N++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 247 GWAWKLANSHQHALFAKADGAASGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 306

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AY Y   TWI +E+  T+A KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 307 VAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Spg003558 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.5e-110
Identity = 211/356 (59.27%), Postives = 260/356 (73.03%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           + E V  GYWLAST+  L    IDA LF+HLF GYA ++ +TY+VT++ S+ ILI+QF+ 
Sbjct: 7   VTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSS 66

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TVK  N +VKT+LSIAG + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
            D+M NF++LI SW + V  +A S + P LLLVATVSNLPYLQNN+QYP  AI   L+WV
Sbjct: 127 QDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWV 186

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP S R +TGPSSAF N  A   SA FGI+ W  + L +L  K+IVFGIPFH
Sbjct: 187 NLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLSHKKIVFGIPFH 246

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKL N++QH +F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 247 GWAWKLANSHQHALFAKADGAASGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 306

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AY Y   TWI +E+  T+A KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 307 VAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Spg003558 vs. ExPASy TrEMBL
Match: A0A6J1G4J9 (acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.5e-110
Identity = 214/356 (60.11%), Postives = 258/356 (72.47%), Query Frame = 0


Query: 1   MAEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTP 60
           MAE V  GYWLA T+  L    IDA LF+HLFVGYA +D  T++VT++ S+ ILINQF+ 
Sbjct: 1   MAEFVNAGYWLAGTDDTLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSS 60

Query: 61  TVKGKNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSS 120
           TVK  N +VKT+LSIA  + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS
Sbjct: 61  TVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSS 120

Query: 121 ADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWV 180
             EM NF++LI SW + V  +A   + P LLLVATVSNLPYLQ N++YP  AI   L+WV
Sbjct: 121 QYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWV 180

Query: 181 NLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 240
           NL+SYDFYTP SS+ +TGPSSAF N  A   SA FGI+ W  + L +LP K+IVFGIPFH
Sbjct: 181 NLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLPHKKIVFGIPFH 240

Query: 241 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQ 300
           GWAWKLFN++QH  F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y 
Sbjct: 241 GWAWKLFNSHQHAPFAKADGAASGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 300

Query: 301 IAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           +AY Y   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 301 VAYAYYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 352

[Close]

BLAST of Spg003558 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.2e-109
Identity = 213/352 (60.51%), Postives = 256/352 (72.73%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           V  GYWLASTN  L    IDA LF+HLFVGYA +D  T++VT++ S+ ILINQF+ TVK 
Sbjct: 11  VNAGYWLASTNDRLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSSTVKL 70

Query: 65  KNGEVKTILSIAGRSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSADEM 124
            N +VKT+LSIA  + VFA +A  PTNRD FI SSIAAAR G FDGLDLQWLYPSS  EM
Sbjct: 71  SNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSSQYEM 130

Query: 125 NNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWVNLVS 184
            NF++LI SW + V  +A   + P LLLVATVSNLPYLQ N++YP  AI   L+WVNL+S
Sbjct: 131 GNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWVNLLS 190

Query: 185 YDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFHGWAW 244
           YDFYTP SS+ +TGPSSAF N  A   SA FGI+ W  + L +LP K+IVFGIPFHGWAW
Sbjct: 191 YDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSW-AQSLHNLPHKKIVFGIPFHGWAW 250

Query: 245 KLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNA-PEETDAAYQIAYT 304
           KLFN++QH  F++ADGAA GE    I   G+I+YSDV+KFI    A  E+ D +Y +AY 
Sbjct: 251 KLFNSHQHAPFAKADGAASGE---YIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 310

Query: 305 YSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
           Y   TWI YE+  TIA KI  AK++ VLGYFAFNIAADD   TLA+AA ++W
Sbjct: 311 YYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Spg003558 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 249.2 bits (635), Expect = 4.9e-66
Identity = 144/357 (40.34%), Postives = 207/357 (57.98%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           V+  YW  ++   ++   ID+ LF+HLF  +A ++  T +VT+ S+     + FT TV+ 
Sbjct: 28  VKASYWFPASEFPVTD--IDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 65  KNGEVKTILSIAG---RSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSA 124
           +N  VKT+LSI G       +A +A  PT+R  FI SSI  AR+  F GLDL W YPSSA
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 125 DEMNNFRSLISSWKEGVTREARSSNRPPLLLVATV--SNLPYLQNNLQYPVAAIKVNLNW 184
            EM NF +L+  W+  V  EA SS +P LLL A V  SN  Y   ++ YPV+A+  +L+W
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 185 VNLVSYDFYTPTSSRISTGPSSAFQNSNA-PQNSALFGIKDWMTKGLTHLPRKQIVFGIP 244
           VNL++YDFY P  SR++  P++ F  SNA P   A  G + W+  G   LP K+ V G P
Sbjct: 208 VNLMAYDFYGPGWSRVTGPPAALFDPSNAGPSGDA--GTRSWIQAG---LPAKKAVLGFP 267

Query: 245 FHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETDAAY 304
           ++G+AW+L NAN H  ++   GAA       IS DG I Y  ++KFI    A    ++  
Sbjct: 268 YYGYAWRLTNANSHSYYAPTTGAA-------ISPDGSIGYGQIRKFIVDNGATTVYNSTV 327

Query: 305 QIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAANASW 356
              Y Y+G  WI Y+   +I  K+  AK   +LGYF++++ ADD+   L+ AA+ +W
Sbjct: 328 VGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

[Close]

BLAST of Spg003558 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-60
Identity = 133/343 (38.78%), Postives = 193/343 (56.27%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           V+  YW A + + L+   ID+ LF+HLF  +A ++  TY+V + S      + FT TV+ 
Sbjct: 26  VKATYWFAESESPLA--QIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 65  KNGEVKTILSIAG---RSDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSA 124
           +N  VKT+LSI G    +  FA +A  PT+R +FI+SSI  AR+  F GLDL W YPS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 125 DEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWVN 184
            EM+NF  L+  W+  V  EARSS +P LLL A V    Y   ++ +PV A+  +L+WVN
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWVN 205

Query: 185 LVSYDFYTPTSSRISTGPSSAFQN-SNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFH 244
           LV+YDFY   SSR++  P+  +   +  P   A  G++ W   G   LP K+ V G P +
Sbjct: 206 LVAYDFYESGSSRVTCSPAPLYDPITTGPSGDA--GVRAWTQAG---LPAKKAVLGFPLY 265

Query: 245 GWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETDAAYQI 304
           G+AW L +A  H  ++ + G A       IS DG I Y  +++FI    A    ++    
Sbjct: 266 GYAWCLTDAKNHNYYANSSGPA-------ISPDGSIGYDQIRRFIVDNKATMVYNSNLVQ 325

Query: 305 AYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDD 344
            Y Y+  TWI Y+   +I  K+  AK   +LGYF+++I ADD+
Sbjct: 326 NYCYAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

[Close]

BLAST of Spg003558 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 231.1 bits (588), Expect = 1.4e-60
Identity = 133/340 (39.12%), Postives = 185/340 (54.41%), Query Frame = 0


Query: 5   VRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPTVKG 64
           V+  YW+         G ID+  F+HLF  +A VD +T+EVTI ++ S  ++ FT TVK 
Sbjct: 14  VKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTVKD 73

Query: 65  KNGEVKTILSIAGR---SDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYPSSA 124
           KN +V+T+LSI G+     V A +A    NR  FI SSI  AR   F GLDL W YPS+ 
Sbjct: 74  KNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSND 133

Query: 125 DEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAIKVNLNWVN 184
            EM NF  L+  W+  V  E+  +N+ PLLL A V   P      +YPV AI  NL++VN
Sbjct: 134 VEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYYGE-EYPVQAIADNLDFVN 193

Query: 185 LVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVFGIPFHG 244
           +++YDFY P  S ++  P++ F  SN    S   G+  W+      LP K+ V G  + G
Sbjct: 194 IMAYDFYGPGWSPVTGPPAALFDPSNPAGRSGDSGLSKWLE---AKLPAKKAVLGFSYCG 253

Query: 245 WAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETDAAYQIA 304
           WAW L +A  +   +  DGAA       IS DG I Y+ ++ +I    A    D A    
Sbjct: 254 WAWTLEDAENNGYDAATDGAA-------ISSDGSITYAKIRNYIIDNGAATFHDPAVIGF 313

Query: 305 YTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAAD 342
           Y Y G TWI Y+   +I  K+  AK + +LGYF++++ AD
Sbjct: 314 YCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

[Close]

BLAST of Spg003558 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 230.7 bits (587), Expect = 1.8e-60
Identity = 141/363 (38.84%), Postives = 201/363 (55.37%), Query Frame = 0


Query: 5   VRGGYWL----ASTNAILSPG----MIDAKLFSHLFVGYAGVDENTYEVTIESSESILIN 64
           V+  YW     + +   LS G     ID+ LF+HLF  +A VD +T+EVTI ++ S   +
Sbjct: 14  VKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQFS 73

Query: 65  QFTPTVKGKNGEVKTILSIAGR---SDVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDL 124
            FT TVK KN +V+T+LSI G+     V A +A    NR  FI SSI  AR   F GLDL
Sbjct: 74  SFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDL 133

Query: 125 QWLYPSSADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQYPVAAI 184
            W YPS+  EM NF  L+  W+  V  E+  +N+ PLLL A V   P   + ++YPV AI
Sbjct: 134 AWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQ-YDGVEYPVKAI 193

Query: 185 KVNLNWVNLVSYDFYTPTSSRISTGPSSAFQN-SNAPQNSALFGIKDWMTKGLTHLPRKQ 244
             NL++VN+++YDFY P  S ++  P++ F + SN    S   G++ W+ +    LP K+
Sbjct: 194 ADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLDE--AKLPPKK 253

Query: 245 IVFGIPFHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPE 304
            V G P+ GWAW L +A  +   +  DGAA       IS DG I Y+ ++ +I    A  
Sbjct: 254 AVLGFPYCGWAWTLEDAENNGYDAATDGAA-------ISPDGSITYAKIRNYIVDNGAAT 313

Query: 305 ETDAAYQIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDDVFTLASAAN 356
             D A    Y Y G TWI Y+   +I  K+  AK   +LGYF++++ AD +   L+ AA+
Sbjct: 314 FHDPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNC-GLSRAAS 365

[Close]

BLAST of Spg003558 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 220.3 bits (560), Expect = 2.4e-57
Identity = 124/348 (35.63%), Postives = 194/348 (55.75%), Query Frame = 0


Query: 2   AEAVRGGYWLASTNAILSPGMIDAKLFSHLFVGYAGVDENTYEVTIESSESILINQFTPT 61
           ++ V+  YW  +T+   +   ID+ LF+HLF  +A ++  +YE+TI +      + FT T
Sbjct: 4   SDVVKASYWFPATDFPATD--IDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTET 63

Query: 62  VKGKNGEVKTILSIAGRS---DVFAKIAEQPTNRDVFITSSIAAARAGQFDGLDLQWLYP 121
           V+ +N  VKT+LSI G +   D FA +A  P +R  FI S+I  AR+  F GLDL W YP
Sbjct: 64  VQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYP 123

Query: 122 SSADEMNNFRSLISSWKEGVTREARSSNRPPLLLVATVSNLPYLQNNLQ---YPVAAIKV 181
            + +EM +F  L+  W+  V  E+ SS    L+L A V    Y  +N Q   YPV AI  
Sbjct: 124 RNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLAISN 183

Query: 182 NLNWVNLVSYDFYTPTSSRISTGPSSAFQNSNAPQNSALFGIKDWMTKGLTHLPRKQIVF 241
           +L+W+NL++YDFY P  S ++  P+S +  ++     +  G++DW   G   LP K+ V 
Sbjct: 184 SLDWINLMAYDFYGPGWSTVTGPPASLYLPTDGRSGDS--GVRDWTEAG---LPAKKAVL 243

Query: 242 GIPFHGWAWKLFNANQHQVFSRADGAARGESSYQISQDGKIYYSDVKKFIATKNAPEETD 301
           G P++GWAW L + + +   +   G A       IS DG+I Y  ++ +I    A +  D
Sbjct: 244 GFPYYGWAWTLADPDVNGYDANTTGPA-------ISDDGEISYRQLQTWIVDNGATKVHD 303

Query: 302 AAYQIAYTYSGPTWIVYETGDTIAKKILSAKSENVLGYFAFNIAADDD 344
                 Y Y+G TWI Y++  +I  K++ AK + +LGYF++++  DD+
Sbjct: 304 DMMVGDYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

The following BLAST results are available for this feature:

BLAST of Spg003558 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023521369.1	5.0e-113	60.39	acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023545402.1	1.9e-112	60.80	acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo]	[more]
XP_022946077.1	4.3e-112	60.39	acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm...	[more]
KAG6599362.1	5.6e-112	60.39	Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1...	[more]
KAG7030350.1	9.5e-112	60.39	Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Spg003558 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	6.9e-65	40.34	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
A0A072UR65	8.5e-47	33.52	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]
U5N4E3	7.9e-45	33.43	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]
A0A1B1J8Z2	8.5e-39	31.58	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]
A0A072VEP0	2.1e-37	29.81	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]

BLAST of Spg003558 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G2P4	2.1e-112	60.39	acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE...	[more]
A0A6J1KFT3	1.5e-110	59.55	chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1	[more]
A0A6J1KB82	2.5e-110	59.27	chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1	[more]
A0A6J1G4J9	2.5e-110	60.11	acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1G4H5	1.2e-109	60.51	chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ...	[more]

BLAST of Spg003558 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19810.1	4.9e-66	40.34	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19820.1	1.1e-60	38.78	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	1.4e-60	39.12	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19760.1	1.8e-60	38.84	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	2.4e-57	35.63	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 4..341 e-value: 2.0E-50 score: 183.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 23..342 e-value: 3.8E-72 score: 245.4
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 3..351
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 3..351
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 239..312 e-value: 3.8E-72 score: 245.4
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 8..341 e-value: 4.7E-50 score: 171.1
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 4..355 score: 42.119537
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 8..348

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003558.1	Spg003558.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding