Spg003499 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003499
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING/U-box superfamily protein
Location	scaffold4: 46952651 .. 46956708 (-)
RNA-Seq Expression	Spg003499
Synteny	Spg003499

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTAATTTTGAGAGTCATCAAAGAGGCACCTGTAAATGTTGCAGGAAGCTTCTTCCCTCCTTCCTTTTTCTTATTTTCTCTCACTACTTTTTTATTTTGTTTAGAGAGAGAAGGAGAGGGAATGATTGAGAAAATCGTTTTGTTATTGTACGTTTTACTCTCACCTTCAATCGCAGGGACCCGCTTTTGTCTGTTACCTTCATCACTCCCCAGCTTCGTTGGCCTTCAATCTTTATCCTTATTTTAATTCCTTCCACTCCCATTTCCCCCACTTTCTTCTTCCTCTTCTTCATTTTTCTGCTTCTGGGTTTCTTCTTTATTTCCTCCAAATGACAGATTGAGTTGTAATTGCTTCTGGGTTTCGGTTAATTTTGATGAAACTGAGTTGAAATCGGTCTGGATTTGAGAATTTTACTGGGTTTTCTTTCTTGTGAAACTCCAGTGATTCTGAGTTGAAATCAGTGAGTGTTTGAGAATCTTACAAGTTTTTCTCTGTTCTTCTTCACTTTTCTGAATTTGAGGAACTTCACTGATTCTGAGTTGAAATCGGTGTGGATTTGATCATTTTTGCAACATGGGTTCTGATTCGTATGCTGAAACTGAGAAACTTGATTGATTTTGAGTCGAAATCCTCTTGGATTCAAGTTTTCACCATTCTCTCTCTGTATTGCTCTGTTTTTCTGCAACTGGGTTTTTCATTCTGAATTGAAATCTTTGTGGGTTAGTTTTTGTGATTTTGAGTAAAAAAACTCCAATCCTCTTCATCTTTTTTCCTGATTTCGTTGAACAATGGCCGGAATGTTGCCCGGAGTTGAATGCGCCAGACGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATTGGGATCCACAAGAAGACCGTCCTTCTGTCTGTACACAAGCAACCATGATTTTCACCTCACTTCATCTTCTTCACAGGTATTAAAAAAAAAATCTAGAAATTCCATCTATATCCTTCGAATTTTCTGTTTGTTTCCTGGGAAAATCCTGATGAATTTTTTGTTAAAAGAAGCAGCGGAGCTCTTCGAATCAAGCTTACCAGAATGAGAAACTTGGTGAAATTGCAAGAGAAGCTAAGGAAAGGTTGGATGAGAGGTTGAGGACGCACAGAAAACCTGAAACTCCGAGGTAATTTCTTCGTGAACCACCTTATTTCTTCCGAATTTTATTCATTGTTCTTCGTATCTAATTTTTTTATTAGATTTTATTAAAATTTAGTCATGAATTCTAGCTCGTATGAATTGAGTCACATTTGGGATGGGAAAATTCGAACTTTTGATCGATCGTAGATGTCAATTACCATTGAGCTATACTCGCTTTGGCGAATTTTATGTTTTTTTTTTTTTTTTTTTTGAAAGATGGCGAATTTTATGTTTATTGAAACTATAAATTTTCAGTTTTGTATTCGGTGGGTTTGCGAATTTTTTAAAAAAATGTTGAATAAGTAAATGATTTATTAGACACAGAATTAAAAGTTGGAGGATTTATTCGAGAAAGTTTAAAAACATATCAGATACTTTTTGAAGTTGAAGGTATTATCGAAAGCTTCGAAGACCTATTTGAACTTTTTAAAGTTCAAGGAATAAATTGACACAATCCTAAAAGTTAAGTGATTAAACATATAATTTAACATTTTTATTATTTTTTCCCAGCTAAAATGTCTTTTCTAGTCATTGTTCCCATATATTTTCAAAGGTCTAAATTTAGACCATATACTTTTTGTAAAATTTATAATTGTTCGCGGTGTTATTTTATTGTTAACTTTTCAAAAAAAAAAAAAAAATAGTAGTCTCTATTGGTCTTTTTTCTTTAAATTTTGTAAATATGTTTTTTTTTTTGCTTGAAAATTATTATTGTTATGTAACAAATGTCGATAATAATTAACTTCAAATTAATGGTAGGAATTAATTTTAAGATTGATGGAAAATCCAAGGATCAAATTTAAACATAAAAAAGTATCAAAATATCGTATCAAGGTGTTCAAATATGTAAAAATAAAATGACAATTTTTTTTTTTAGAGGTAAAATGATAATTTTAAGATGGGGATTCAAACTCAAGACCTTAGTTATAAGTACATATTTTATGTCAGCTGAGTTATATTCTGGTTGGCGAATAAACAATAATTGACCAATCAATTGAAGACAAATCATATAGAAGTTGACACGTGACTTACCCGTATTGAGTTATATGCTTACAAGTTACAATAATTGAACTCAAATTTATTGAATCTGAAGAGACTTGATTAGAATAGTTTGGTTGGTTTTCATTTATAACATTGCCAACCTCTATTATATAGTTTTAATTGAATAGGAATTTAAAAAGAAATTGAATATGATTAGTACTTCTAATCTATTTAGTCCTAAAATTTATAAAATTTTCATTTAGTCCTTCAATTTGTAACTGGTTTTTCATTTTTGCTCCTTTTAGTTTTCATAATTTTGATTTTAGCTCACTAATTTTCACGATATTTTATGTAGTCTCTCGCATTAATATCTTACTTCTTTTAACTTTACAATGTTTTTTTAAGTCTACCGTTTTAAGCATATGCCATTAATCAAGAGATTAAGGTTCAATTCTTCTTTCTATAGCTTGTTGACCTAAAAAAAAAATCTTTTTCTGACTTTCTAATTTCCTATATTTTACTTTTATTTTCTTTTAGTCCTCCTACCCTTAAATGCTTTGTTTAATTATTGAACTTTACGCTTAAAACAAAACTATTTTAACGGTTGCATTCATTTTCATTCAATTATTGAACATAAATCAAAATTTAAAATTTAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCTTAGCCTACTACAAAATGTGAATTGAAATCTATTTATTTAATTGATGAATAAACAGAAACAAGGCCTATATATATATATATATTTTTTTTTTTTTTTGAAACAAGGCCTATATTAAGTTTCCGTCAAATAATTAAAAACAAGAATTTTACAACAAAAATAAATATTTTTTTATAGATAATAATAACAAAGAAAGGAAAAGATACATAGAAATATTTTTCAAAAAAAAAAAAAAGATACATAGAAATATGAAAAGAACAAAATTATGAATATCAAACCTTTTAAAATCTGATCGATATTCTAAAATTATATTACAAAACAAAGACAAACTTTAGGTACACAAAATCAAGGTCAAATCACATTATTTGGTTATCCATATGGTTTGAGCAATGGTTTTGATTCAGTCCTCTTAGTTTACATAATTTGAATCTAATTTTAGTTTAGTGGCTAAGGTTTTAAAATTTTCAATTTGATCTCTATAGTTTAAATAAATCTCACAATTAAATCGTACCTAAATTATCTTTATCCACTTAAAAAGTCAACTCACTTCCTTTTATTATTTTTTTTTCAACTAACTCACAGTTACAAACGAGCCATAAGAATTCGACTAAGACGATCAAATCAAAACTCTTAAAACTATAAATACGAAGTTGAAACTATGTGCAAACAGACGAAAACCACGATCAAATTTAAATATCTTTTTTCTTTTTGTTTGTACAATTATAGGAAAAATAGTAGTGAAAAAATAGGGTTGAAGGGATCATCGGAGAAAACAAAGGGGAAAGAAGAAACAACAACAAAGAAGAGGTTCAGTTGGGGGAAGCTAATGAAATGGAAGGCCTCAGAGCAAGAAGAGTGCGCCGTTTGTCTCGAAGGGTTCAGAGCTGGCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCTCCTGCTTGGTTCCCTGGCTCCGAGCCAATTCCCATTGCCCTTGCTGCAGATTCCCCATTCCCTCTCAATAACAAGACTCTCTTCCCTTTTTTTTTTTTTTACTCTTTATGTAAACGAGAAATTATTCGAGGGGATAAAATAATGTGGTTAACGGTTTTGTACTGTTAGATGTATGTGAGAAATAACATTATTGTAGTTTAACCGTGGATTATTATTGAAGGGTTTATAGTTGTCGCATTAAATAATTTCTCTCTCTTTGTGTT

mRNA sequence

ATGGCCGGAATGTTGCCCGGAGTTGAATGCGCCAGACGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATTGGGATCCACAAGAAGACCGTCCTTCTGTCTGTACACAAGCAACCATGATTTTCACCTCACTTCATCTTCTTCACAGAAGCAGCGGAGCTCTTCGAATCAAGCTTACCAGAATGAGAAACTTGGTGAAATTGCAAGAGAAGCTAAGGAAAGGTTGGATGAGAGGTTGAGGACGCACAGAAAACCTGAAACTCCGAGGAAAAATAGTAGTGAAAAAATAGGGTTGAAGGGATCATCGGAGAAAACAAAGGGGAAAGAAGAAACAACAACAAAGAAGAGGTTCAGTTGGGGGAAGCTAATGAAATGGAAGGCCTCAGAGCAAGAAGAGTGCGCCGTTTGTCTCGAAGGGTTCAGAGCTGGCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCTCCTGCTTGGTTCCCTGGCTCCGAGCCAATTCCCATTGCCCTTGCTGCAGATTCCCCATTCCCTCTCAATAA

Coding sequence (CDS)

ATGGCCGGAATGTTGCCCGGAGTTGAATGCGCCAGACGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATTGGGATCCACAAGAAGACCGTCCTTCTGTCTGTACACAAGCAACCATGATTTTCACCTCACTTCATCTTCTTCACAGAAGCAGCGGAGCTCTTCGAATCAAGCTTACCAGAATGAGAAACTTGGTGAAATTGCAAGAGAAGCTAAGGAAAGGTTGGATGAGAGGTTGAGGACGCACAGAAAACCTGAAACTCCGAGGAAAAATAGTAGTGAAAAAATAGGGTTGAAGGGATCATCGGAGAAAACAAAGGGGAAAGAAGAAACAACAACAAAGAAGAGGTTCAGTTGGGGGAAGCTAATGAAATGGAAGGCCTCAGAGCAAGAAGAGTGCGCCGTTTGTCTCGAAGGGTTCAGAGCTGGCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCTCCTGCTTGGTTCCCTGGCTCCGAGCCAATTCCCATTGCCCTTGCTGCAGATTCCCCATTCCCTCTCAATAA

Protein sequence

MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSSQKQRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKGSSEKTKGKEETTTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPIPSQ

Homology

[Close]

BLAST of Spg003499 vs. NCBI nr
Match: KAG7030344.1 (putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321.6 bits (823), Expect = 4.4e-84
Identity = 164/191 (85.86%), Postives = 173/191 (90.58%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSS-QKQ 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAKLGSTRRPSFCLYTSN DFH+TSSSS  +Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKGS---SEKTKGK 120
           RSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPE PRK S E  GLK S    EK KGK
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE--GLKNSLEKKEKAKGK 120

Query: 121 EETTTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANS 180
           EE+ TKKRF+WGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHS+CLVPWL+ N+
Sbjct: 121 EESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNA 180

Query: 181 HCPCCRFPIPS 188
           HCPCCRFP PS
Sbjct: 181 HCPCCRFPFPS 189

[Close]

BLAST of Spg003499 vs. NCBI nr
Match: XP_038891102.1 (probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida])

HSP 1 Score: 317.4 bits (812), Expect = 8.4e-83
Identity = 164/197 (83.25%), Postives = 174/197 (88.32%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSSQKQR 60
           MAGMLPGVECARRRRFHQSI SSDSPSTAAK GSTRRPSFCLYTSN DFH TSSSS + R
Sbjct: 1   MAGMLPGVECARRRRFHQSIASSDSPSTAAKSGSTRRPSFCLYTSNRDFHSTSSSSSQHR 60

Query: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPE-TPRKNSSEKIGLKGSSEK------- 120
           SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPE + RK+ +EK+G KGSSEK       
Sbjct: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENSRRKSENEKVGWKGSSEKNGEEKER 120

Query: 121 TKGKEET--TTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVP 180
           TKGKEE+   TKKRF+WGKLMKW+ASEQEEC VCLE FR GEPLVHLPCAHKFHS+CLVP
Sbjct: 121 TKGKEESIIITKKRFNWGKLMKWRASEQEECTVCLERFRVGEPLVHLPCAHKFHSTCLVP 180

Query: 181 WLRANSHCPCCRFPIPS 188
           WL+ANSHCPCCRFPIPS
Sbjct: 181 WLQANSHCPCCRFPIPS 197

[Close]

BLAST of Spg003499 vs. NCBI nr
Match: XP_023545449.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 314.3 bits (804), Expect = 7.1e-82
Identity = 160/189 (84.66%), Postives = 168/189 (88.89%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLT--SSSSQK 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSN DFH+T  SSSS +
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60

Query: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKGSSEKTKGKEE 120
           QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPE PRK S E     GS      KEE
Sbjct: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE-----GS------KEE 120

Query: 121 TTTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHC 180
           + TKKRF+WGKLMKWKASEQEEC +CLEGFRAGEPLVHLPCAHKFHS+CLVPWL+AN+HC
Sbjct: 121 SITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVPWLQANAHC 178

Query: 181 PCCRFPIPS 188
           PCCRFP PS
Sbjct: 181 PCCRFPFPS 178

[Close]

BLAST of Spg003499 vs. NCBI nr
Match: XP_023538036.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 312.4 bits (799), Expect = 2.7e-81
Identity = 162/189 (85.71%), Postives = 171/189 (90.48%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSSQKQR 60
           MAGMLPGVECARRRRFHQSITSSDS STAAKLGSTRR SFCLYTSN DFHLTSSSSQ QR
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQ-QR 60

Query: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPR-KNSSEKIGLKGSSEKTKGKEET 120
            SSN AYQNEKLG+ AREAKERLDERLRTHRKPE  R K+SSE++GLK   EK KGKEET
Sbjct: 61  CSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLK--KEKAKGKEET 120

Query: 121 TTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCP 180
           TTKKRFSWGKLMKWKASEQEEC VCLE FRA EPLVHLPCAHKFHS+CLVPWL+AN+HCP
Sbjct: 121 TTKKRFSWGKLMKWKASEQEECTVCLERFRAVEPLVHLPCAHKFHSTCLVPWLQANAHCP 180

Query: 181 CCRFPIPSQ 189
           CCR+PI +Q
Sbjct: 181 CCRYPILAQ 186

[Close]

BLAST of Spg003499 vs. NCBI nr
Match: XP_022946687.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata])

HSP 1 Score: 310.5 bits (794), Expect = 1.0e-80
Identity = 157/188 (83.51%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSS-QKQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAKLGSTRRPSFCLYTSN DFH+TSSSS  +Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKGSSEKTKGKEET 120
           RSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPE PRK S E             KEE+
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE-----------GWKEES 120

Query: 121 TTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCP 180
            TKKRF+WGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHS+CLVPWL+ N+HCP
Sbjct: 121 ITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCP 177

Query: 181 CCRFPIPS 188
           CCRFP PS
Sbjct: 181 CCRFPFPS 177

[Close]

BLAST of Spg003499 vs. ExPASy Swiss-Prot
Match: Q852U6 (Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RHY1A PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 30/53 (56.60%), Postives = 36/53 (67.92%), Query Frame = 0


Query: 136 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPIPSQ 189
           SE  +C++CLE F  G+ L+ LPC H FHSSCL PWLRA   CPCCR  I  +
Sbjct: 198 SEMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAIAKE 250

[Close]

BLAST of Spg003499 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.2e-11
Identity = 32/83 (38.55%), Postives = 45/83 (54.22%), Query Frame = 0


Query: 106 LKGSSEKTKGKEETTTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHS 165
           L+  +E    +  T   K+ +   L   K  E   C+VCL+    G     +PC HKFHS
Sbjct: 164 LQHLAESDPSRNGTPPAKKEAVEALPTVKIEEVVSCSVCLDDLEVGSQAKQMPCEHKFHS 223

Query: 166 SCLVPWLRANSHCPCCRFPIPSQ 189
           SC++PWL  +S CP CRF +PS+
Sbjct: 224 SCILPWLELHSSCPVCRFELPSE 246

[Close]

BLAST of Spg003499 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.9e-11
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 136 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPIPS 188
           SE  +CAVC++ F  G  +  +PC H FH  CL+PWL  ++ CP CRF +P+
Sbjct: 211 SEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of Spg003499 vs. ExPASy Swiss-Prot
Match: Q9C1X4 (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP32A8.03c PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.8e-10
Identity = 22/49 (44.90%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 137 EQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPI 186
           E+ EC +C+E F+  + ++ LPC H FH +C+ PWLR N  C  CR P+
Sbjct: 392 EEGECTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPV 440

[Close]

BLAST of Spg003499 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.7e-09
Identity = 22/52 (42.31%), Postives = 32/52 (61.54%), Query Frame = 0


Query: 136 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPIPS 188
           SE  +CAVC++ F  G     +PC H +H  CL+PWL  ++ CP CR  +P+
Sbjct: 219 SEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPT 270

[Close]

BLAST of Spg003499 vs. ExPASy TrEMBL
Match: A0A6J1G4D6 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC111450678 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 4.9e-81
Identity = 157/188 (83.51%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSS-QKQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAKLGSTRRPSFCLYTSN DFH+TSSSS  +Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKGSSEKTKGKEET 120
           RSSS+QAYQNEKLGEIAREAKERLDERLRTHRKPE PRK S E             KEE+
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE-----------GWKEES 120

Query: 121 TTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCP 180
            TKKRF+WGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHS+CLVPWL+ N+HCP
Sbjct: 121 ITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCP 177

Query: 181 CCRFPIPS 188
           CCRFP PS
Sbjct: 181 CCRFPFPS 177

[Close]

BLAST of Spg003499 vs. ExPASy TrEMBL
Match: A0A6J1HQI5 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 9.3e-80
Identity = 159/189 (84.13%), Postives = 168/189 (88.89%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSSQKQR 60
           MAGMLPGVECARRRRFHQSITSS+S STAAKLGSTRR SFCLYTSN DFHLTSSSSQ QR
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQ-QR 60

Query: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPR-KNSSEKIGLKGSSEKTKGKEET 120
            SSN AYQNEKLG+ AREAKERLDERLRTHRKPE  R K+SSE++ LK   E  KGKEET
Sbjct: 61  CSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLK--KENVKGKEET 120

Query: 121 TTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCP 180
           TTKKRFSWGKLMKWKASEQEEC VCLE FRA EPLVHLPCAHKFHS CLVPWL+AN+HCP
Sbjct: 121 TTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCP 180

Query: 181 CCRFPIPSQ 189
           CCR+PI +Q
Sbjct: 181 CCRYPILAQ 186

[Close]

BLAST of Spg003499 vs. ExPASy TrEMBL
Match: A0A6J1FHS8 (E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444265 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.1e-79
Identity = 158/189 (83.60%), Postives = 169/189 (89.42%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSSQKQR 60
           MAGMLPGVECARRRRFHQSITSSDS STAAKLGSTRR SFCLY SN DFHLTSSSSQ QR
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHLTSSSSQ-QR 60

Query: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPR-KNSSEKIGLKGSSEKTKGKEET 120
            SSN AYQ+EKLG+ AREAKERLDERLRTHRKPE  R K+SSE++GLK   EK KGKEET
Sbjct: 61  CSSNHAYQDEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLK--KEKAKGKEET 120

Query: 121 TTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCP 180
           TTKKRF WGKLMKWKASEQEEC VCLE FRA EPLVHLPCAHKFHS+CLVPWL+AN++CP
Sbjct: 121 TTKKRFGWGKLMKWKASEQEECTVCLEKFRATEPLVHLPCAHKFHSACLVPWLQANAYCP 180

Query: 181 CCRFPIPSQ 189
           CCR+PI +Q
Sbjct: 181 CCRYPILAQ 186

[Close]

BLAST of Spg003499 vs. ExPASy TrEMBL
Match: A0A6J1KK21 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita maxima OX=3661 GN=LOC111493891 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.1e-77
Identity = 153/186 (82.26%), Postives = 163/186 (87.63%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSS---Q 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSN DFH+TSSSS    
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSRS 60

Query: 61  KQRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKGSSEKTKGKE 120
           +QRSSSNQAYQ EKLGEIAREAKERLDERL++HRKP+ PRK S E     GS      KE
Sbjct: 61  QQRSSSNQAYQTEKLGEIAREAKERLDERLKSHRKPQNPRKKSGE-----GS------KE 120

Query: 121 ETTTKKRFSWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSH 180
           E+ TKKRF+WGKLMKWKASEQ+ECAVCLEGFRAGEPLVHLPCAHKFHS+C VPWL+AN H
Sbjct: 121 ESITKKRFTWGKLMKWKASEQQECAVCLEGFRAGEPLVHLPCAHKFHSACFVPWLQANHH 175

Query: 181 CPCCRF 184
           CPCCRF
Sbjct: 181 CPCCRF 175

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BLAST of Spg003499 vs. ExPASy TrEMBL
Match: A0A0A0LG47 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.8e-75
Identity = 157/202 (77.72%), Postives = 169/202 (83.66%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNHDFHLTSSSSQKQR 60
           MAGMLPGVECARRRRFHQSI SSDSP TA K GSTRRPSFCLYTSN DFHL SSSS +QR
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPETPR-KNSSEKIGLKGSSEK------- 120
           S +NQAYQN+KLGEIAREAKERLDERLRTHRKPE  R KN++E++  K S EK       
Sbjct: 61  SLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKKNNNEEVKGKESREKNNGEKKE 120

Query: 121 ---TKGKEETTTKKRFSWG-KLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCL 180
              TKGKEE  TKKRFSWG KLMKWKASEQEEC+VCLE FR  EPL+HLPCAHKFHS+CL
Sbjct: 121 MKNTKGKEE-ETKKRFSWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTCL 180

Query: 181 VPWLRANSHCPCCRFPI--PSQ 189
           VPWL+AN+HCPCCRFPI  PSQ
Sbjct: 181 VPWLQANAHCPCCRFPIAVPSQ 201

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BLAST of Spg003499 vs. TAIR 10
Match: AT2G15580.1 (RING/U-box superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-36
Identity = 99/199 (49.75%), Postives = 127/199 (63.82%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNHDFHLTSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+NH+ H  +  
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESH-QAHV 60

Query: 61  SQKQRSSSNQAY---QNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKGSSEK 120
           S  +RS  N++Y    +EKL   A+EAK+RL++RLR    P   R+N  +K G K    K
Sbjct: 61  SFSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRI---PPRTRQNGKDK-GNKLEQGK 120

Query: 121 TKGKEETTTK---KRFSWGKLMKW---KASEQEECAVCLEGFRAGEPLVHLPCAHKFHSS 180
            K   +  T+    + S G+LM+W   +  EQ++CA+CL+ F+ GE LVHLPCAHKFHS 
Sbjct: 121 GKPLGDLPTEVVGLKKSRGRLMEWFKRRVREQQDCAICLDRFKKGETLVHLPCAHKFHSI 180

Query: 181 CLVPWLRANSHCPCCRFPI 186
           CL+PWL  N +CP CR  I
Sbjct: 181 CLLPWLDTNVYCPYCRTDI 194

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BLAST of Spg003499 vs. TAIR 10
Match: AT2G15580.2 (RING/U-box superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-15
Identity = 58/116 (50.00%), Postives = 74/116 (63.79%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNHDFHLTSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+NH+ H  +  
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESH-QAHV 60

Query: 61  SQKQRSSSNQAY---QNEKLGEIAREAKERLDERLRTHRKPETPRKNSSEKIGLKG 109
           S  +RS  N++Y    +EKL   A+EAK+RL++RLR       PR +S + +  KG
Sbjct: 61  SFSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRI-----PPRTSSGKMVKTKG 110

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BLAST of Spg003499 vs. TAIR 10
Match: AT1G49850.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.3e-14
Identity = 30/53 (56.60%), Postives = 36/53 (67.92%), Query Frame = 0


Query: 136 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPIPSQ 189
           SE  +C++CLE F  G+ L+ LPC H FHSSCL PWLRA   CPCCR  I  +
Sbjct: 198 SEMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAIAKE 250

[Close]

BLAST of Spg003499 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 6.3e-12
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 136 SEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPIPS 188
           SE  +CAVC++ F  G  +  +PC H FH  CL+PWL  ++ CP CRF +P+
Sbjct: 211 SEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of Spg003499 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 8.2e-12
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 0


Query: 134 KASEQEECAVCLEGFRAGEPLVHLPCAHKFHSSCLVPWLRANSHCPCCRFPIP 187
           K SE  +C++CL+ F  G     +PC HKFH  C+VPWL  +S CP CR+ +P
Sbjct: 234 KISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYELP 286

The following BLAST results are available for this feature:

BLAST of Spg003499 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030344.1	4.4e-84	85.86	putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subs...	[more]
XP_038891102.1	8.4e-83	83.25	probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida]	[more]
XP_023545449.1	7.1e-82	84.66	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo]	[more]
XP_023538036.1	2.7e-81	85.71	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo]	[more]
XP_022946687.1	1.0e-80	83.51	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata]	[more]

BLAST of Spg003499 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q852U6	3.3e-13	56.60	Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q6AVN2	5.2e-11	38.55	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	8.9e-11	46.15	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9C1X4	9.8e-10	44.90	Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra...	[more]
P0CH30	3.7e-09	42.31	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]

BLAST of Spg003499 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4D6	4.9e-81	83.51	probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1HQI5	9.3e-80	84.13	probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=366...	[more]
A0A6J1FHS8	2.1e-79	83.60	E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1KK21	1.1e-77	82.26	probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0LG47	1.8e-75	77.72	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 P...	[more]

BLAST of Spg003499 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15580.1	1.6e-36	49.75	RING/U-box superfamily protein	[more]
AT2G15580.2	2.7e-15	50.00	RING/U-box superfamily protein	[more]
AT1G49850.1	2.3e-14	56.60	RING/U-box superfamily protein	[more]
AT3G19950.1	6.3e-12	46.15	RING/U-box superfamily protein	[more]
AT4G26400.2	8.2e-12	47.17	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 141..181 e-value: 2.9E-5 score: 33.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 140..182 e-value: 8.5E-12 score: 45.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 141..182 score: 12.371254
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 114..187 e-value: 1.5E-18 score: 67.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 20..35
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 73..120
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 16..35
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 52..120
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 52..70
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 1..182
None	No IPR available	PANTHER	PTHR22765:SF204	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..182
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 140..182 e-value: 3.31113E-18 score: 72.3345
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 129..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003499.1	Spg003499.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
molecular_function	GO:0061630	ubiquitin protein ligase activity