Spg003475 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003475 vs. NCBI nr
Match: KAG6586420.1 (putative ADP-ribosylation factor GTPase-activating protein AGD14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1258.4 bits (3255), Expect = 0.0e+00
Identity = 647/752 (86.04%), Postives = 669/752 (88.96%), Query Frame = 0

Query: 23  LNSSRLAYMANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSG 82
           LN  +LAYMANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSG
Sbjct: 11  LNKKKLAYMANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSG 70

Query: 83  IHREFTHRVKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDF 142
           IHREFTHRVKSISMAKFTSQEV ALQEGGNQRA+EIYFKELDPQRHSFPDSSNVMRLRDF
Sbjct: 71  IHREFTHRVKSISMAKFTSQEVSALQEGGNQRAREIYFKELDPQRHSFPDSSNVMRLRDF 130

Query: 143 IKHVYVDRRYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRR 202
           IKHVYVDRRYSGDKNFDKPPRVK                          GEKEDY ENRR
Sbjct: 131 IKHVYVDRRYSGDKNFDKPPRVK-------------------------SGEKEDYNENRR 190

Query: 203 TDSYQGGSRSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDW 262
           TD+Y+GGSRSPPY+DR YNE+SSPGGRNFD RRSPGSDHEN QFGDF  SP+RP+VVNDW
Sbjct: 191 TDTYRGGSRSPPYEDRHYNERSSPGGRNFDGRRSPGSDHENRQFGDF-MSPSRPEVVNDW 250

Query: 263 RREDRFGNGKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVI 322
           RREDRFGNGKRVED R SDG+SKIGGRSPE+PNDLDA+S PMVRPVRDILGEN+SPLRVI
Sbjct: 251 RREDRFGNGKRVEDARSSDGDSKIGGRSPEQPNDLDASSSPMVRPVRDILGENVSPLRVI 310

Query: 323 EPPKSSGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVT 382
           EPPK SG SK AD+SVHKQRTASSSSLGSISE+VVETKLEPSGSLIDFDAEP+PI SAV 
Sbjct: 311 EPPKISGGSKAADSSVHKQRTASSSSLGSISESVVETKLEPSGSLIDFDAEPEPIASAVP 370

Query: 383 QPPQNSAPQSVTHPVNSNNDNNWASFDVPSQAP-----PAPANVGTLESVLSQLSVSASV 442
           QPPQ+SAPQSVTH VNSNNDNNWASFDVP   P     PAPANVGTLESVLSQLSVSASV
Sbjct: 371 QPPQSSAPQSVTHAVNSNNDNNWASFDVPQAPPAPAPAPAPANVGTLESVLSQLSVSASV 430

Query: 443 PGVSGSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTF 502
           PGVSGSHGA GAVPTAPV NM ML  GFDPSFGSGGNT MPPPFAGGAPSAAPG GLSTF
Sbjct: 431 PGVSGSHGAVGAVPTAPVSNMMMLPCGFDPSFGSGGNTLMPPPFAGGAPSAAPGTGLSTF 490

Query: 503 PPSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNPAAH- 562
           PPSGQW NMQ QTHS FPGGN+QP GQQFPPSIDRSINQPWNASLSAN QGP SNPAAH 
Sbjct: 491 PPSGQWSNMQSQTHSLFPGGNSQPGGQQFPPSIDRSINQPWNASLSANSQGPLSNPAAHA 550

Query: 563 -APQDFSSPAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPM 622
            APQDFSSPAQG ASGVPQTSAPEVKP+GRKELPADLF LNYSSYPAPVPGWH++PPRPM
Sbjct: 551 PAPQDFSSPAQGTASGVPQTSAPEVKPSGRKELPADLFALNYSSYPAPVPGWHTIPPRPM 610

Query: 623 GYVMPYNSAMPVPSFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYG 682
           GYVMPYNS MP+PSFPQ S STNPFDLSSEQSQFPSMAPLQNA PSGQP SNL  TPGYG
Sbjct: 611 GYVMPYNSPMPMPSFPQPSISTNPFDLSSEQSQFPSMAPLQNALPSGQPGSNLIRTPGYG 670

Query: 683 NPASTWIPHQPPTNLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGAT 742
           NPAS WIP QPP+NLS LPPQASYPSTM SRPPYMGQQVASNVQPYRHPGLGNYGNEG  
Sbjct: 671 NPASAWIPQQPPSNLSHLPPQASYPSTMHSRPPYMGQQVASNVQPYRHPGLGNYGNEGVA 730

Query: 743 HGYTDMSQQTAGRFSAPATPNPFSSTGGNPFG 768
           HGYTDMSQQTAGRF APATPNPFSSTGGNPFG
Sbjct: 731 HGYTDMSQQTAGRFPAPATPNPFSSTGGNPFG 736

BLAST of Spg003475 vs. NCBI nr
Match: XP_038890018.1 (probable ADP-ribosylation factor GTPase-activating protein AGD14 isoform X2 [Benincasa hispida])

HSP 1 Score: 1250.7 bits (3235), Expect = 0.0e+00
Identity = 636/738 (86.18%), Postives = 662/738 (89.70%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRAKEIYFKELDPQRHSFPDSSNV+RLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAKEIYFKELDPQRHSFPDSSNVVRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFDKPPRVK                          GEKED+YE RRTD+Y GGS
Sbjct: 121 RYSGDKNFDKPPRVK-------------------------SGEKEDFYETRRTDTYAGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DRRYNE+SSPGGRNFDERRSPGSDHEN QFGDFR+SPAR +VVNDWRREDRF N
Sbjct: 181 RSPPYEDRRYNERSSPGGRNFDERRSPGSDHENRQFGDFRKSPARSEVVNDWRREDRFEN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVEDGRLSDG+SKIGGRSPERPNDLDA+SPP VRPVRDILGEN+SPLRVIEPPKSSGT
Sbjct: 241 GKRVEDGRLSDGDSKIGGRSPERPNDLDASSPPTVRPVRDILGENVSPLRVIEPPKSSGT 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           SKV D+SVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEP+PITSAV QPPQ+SAP
Sbjct: 301 SKVPDSSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPEPITSAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAPPAPANVGTLESVLSQLSVSASVPGVSGSHGATGAV 450
           Q +TH VNS +DNNWASFDV  QAPPAPANVGTLESVLSQLSVS SVPGVSG H A GA+
Sbjct: 361 QPITHTVNSTSDNNWASFDVAPQAPPAPANVGTLESVLSQLSVSGSVPGVSGLHAAAGAI 420

Query: 451 PTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPNMQLQT 510
           P APVGNMT+L TGFDPSFGSGGNTHMPPPF+GGAPSAAPGAGLSTFPPSGQW NMQ QT
Sbjct: 421 PNAPVGNMTILPTGFDPSFGSGGNTHMPPPFSGGAPSAAPGAGLSTFPPSGQWSNMQSQT 480

Query: 511 HSSFPGGNTQPVGQQFPPSIDRSINQ-PWNASLSANPQGPQSNPAAHAPQDFSSPAQGIA 570
           HS FPGGN+QPVGQQFPPSIDRSIN  PWNA  S N QGP SNPAAHAPQDFS+P+Q I 
Sbjct: 481 HSLFPGGNSQPVGQQFPPSIDRSINHAPWNA--SQNSQGPLSNPAAHAPQDFSTPSQAIT 540

Query: 571 SGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNSAMPVPS 630
           SGVPQTSAPEVKP+GRKELPADLFTLNYSSYPAPVPGWH+VPPRPM Y MPYNSAMPVPS
Sbjct: 541 SGVPQTSAPEVKPSGRKELPADLFTLNYSSYPAPVPGWHTVPPRPMVYAMPYNSAMPVPS 600

Query: 631 FPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIPHQPPTN 690
           FPQSSTSTNPFDLSSEQ QFPSMA LQNA PSGQP SNL H PGYGNP STW+PHQPP+N
Sbjct: 601 FPQSSTSTNPFDLSSEQPQFPSMASLQNALPSGQPTSNLIHNPGYGNPTSTWMPHQPPSN 660

Query: 691 LSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQQTAGRF 750
           L QLPPQ  YPSTMPSRPPYMGQ VASNVQPYRHPGL NYGNEGA + YTDMSQQT GRF
Sbjct: 661 LLQLPPQGPYPSTMPSRPPYMGQHVASNVQPYRHPGLSNYGNEGAANAYTDMSQQTGGRF 711

Query: 751 SAPATPNPFSSTGGNPFG 768
           SAPA PNPFSSTGGNPFG
Sbjct: 721 SAPADPNPFSSTGGNPFG 711

BLAST of Spg003475 vs. NCBI nr
Match: KAG7021276.1 (putative ADP-ribosylation factor GTPase-activating protein AGD14 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 642/744 (86.29%), Postives = 663/744 (89.11%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRA+EIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAREIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFDKPPRVK                          GEKEDY ENRRTD+Y+GGS
Sbjct: 121 RYSGDKNFDKPPRVK-------------------------SGEKEDYNENRRTDTYRGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DR YNE+SSPGGRNFD RRSPGSDHEN QFGDF  SP+RP+VVNDWRREDRFGN
Sbjct: 181 RSPPYEDRHYNERSSPGGRNFDGRRSPGSDHENRQFGDF-MSPSRPEVVNDWRREDRFGN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVED R SDG+SKIGGRSPE+PNDLDA+S PMVRPVRDILGEN+SPLRVIEPPK SG 
Sbjct: 241 GKRVEDARSSDGDSKIGGRSPEQPNDLDASSSPMVRPVRDILGENVSPLRVIEPPKISGG 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           SK AD+SVHKQRTASSSSLGSISE+VVETKLEPSGSLIDFDAEP+PI SAV QPPQ+SAP
Sbjct: 301 SKAADSSVHKQRTASSSSLGSISESVVETKLEPSGSLIDFDAEPEPIASAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAP-----PAPANVGTLESVLSQLSVSASVPGVSGSHG 450
           QSVTH VNSNNDNNWASFDVP   P     PAPANVGTLESVLSQLSVSASVPGVSGSHG
Sbjct: 361 QSVTHAVNSNNDNNWASFDVPQAPPAPAPAPAPANVGTLESVLSQLSVSASVPGVSGSHG 420

Query: 451 ATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPN 510
           A GAVPTAPV NM ML  GFDPSFGSGGNT MPPPFAGGAPSAAPG GLSTFPPSGQW N
Sbjct: 421 AVGAVPTAPVSNMMMLPCGFDPSFGSGGNTLMPPPFAGGAPSAAPGTGLSTFPPSGQWSN 480

Query: 511 MQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNPAAH--APQDFSS 570
           MQ QTHS FPGGN+QP GQQFPPSIDRSINQPWNASLSAN QGP SNPAAH  APQDFSS
Sbjct: 481 MQSQTHSLFPGGNSQPGGQQFPPSIDRSINQPWNASLSANSQGPLSNPAAHAPAPQDFSS 540

Query: 571 PAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNS 630
           PAQG ASGVPQTSAPEVKP+GRKELPADLF LNYSSYPAPVPGWH++PPRPMGYVMPYNS
Sbjct: 541 PAQGTASGVPQTSAPEVKPSGRKELPADLFALNYSSYPAPVPGWHTIPPRPMGYVMPYNS 600

Query: 631 AMPVPSFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIP 690
            MP+PSFPQ S STNPFDLSSEQSQFPSMAPLQNA PSGQP SNL  TPGYGNPAS WIP
Sbjct: 601 PMPMPSFPQPSISTNPFDLSSEQSQFPSMAPLQNALPSGQPGSNLIRTPGYGNPASAWIP 660

Query: 691 HQPPTNLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQ 750
            QPP+NLS LPPQASYPSTM SRPPYMGQQVASNVQPYRHPGLGNYGNEG  HGYTDMSQ
Sbjct: 661 QQPPSNLSHLPPQASYPSTMHSRPPYMGQQVASNVQPYRHPGLGNYGNEGVAHGYTDMSQ 718

Query: 751 QTAGRFSAPATPNPFSSTGGNPFG 768
           QTAGRF APATPNPFSSTGGNPFG
Sbjct: 721 QTAGRFPAPATPNPFSSTGGNPFG 718

BLAST of Spg003475 vs. NCBI nr
Match: XP_022965600.1 (probable ADP-ribosylation factor GTPase-activating protein AGD14 [Cucurbita maxima] >XP_022965601.1 probable ADP-ribosylation factor GTPase-activating protein AGD14 [Cucurbita maxima])

HSP 1 Score: 1247.3 bits (3226), Expect = 0.0e+00
Identity = 641/744 (86.16%), Postives = 665/744 (89.38%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRA+EIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAREIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFDKPPRVK                          GEKEDY ENRRTD+Y+GGS
Sbjct: 121 RYSGDKNFDKPPRVK-------------------------SGEKEDYNENRRTDTYRGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DRRYNE+SSPGGRNFD RRSPGSDHEN QFGDF  SP+RP+VVNDWRREDRFGN
Sbjct: 181 RSPPYEDRRYNERSSPGGRNFDGRRSPGSDHENRQFGDF-MSPSRPEVVNDWRREDRFGN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVED R SDG+SKIGGRSPE+PNDLDA+SPPMVRPVRDILGEN+SPLRVIEPPK SG 
Sbjct: 241 GKRVEDARSSDGDSKIGGRSPEQPNDLDASSPPMVRPVRDILGENVSPLRVIEPPKISGG 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           SK AD+SVHKQRTASSSSLGSISE+VVETKLEPSGSLIDFDAEP+PI SAV QPPQ+SAP
Sbjct: 301 SKAADSSVHKQRTASSSSLGSISESVVETKLEPSGSLIDFDAEPEPIASAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAP-----PAPANVGTLESVLSQLSVSASVPGVSGSHG 450
           QSVTH VN+N+DNNWASFDVP   P     PAPANVGTLESVLSQLSVSASVPGVSGSHG
Sbjct: 361 QSVTHAVNTNSDNNWASFDVPQAPPAPASAPAPANVGTLESVLSQLSVSASVPGVSGSHG 420

Query: 451 ATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPN 510
           A GAVPTAPV NMTML +GFDPSFGSGGNT MPPPFAGGAPSAAPG GLSTFPPSGQW N
Sbjct: 421 AVGAVPTAPVSNMTMLPSGFDPSFGSGGNTLMPPPFAGGAPSAAPGTGLSTFPPSGQWSN 480

Query: 511 MQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNPAAHA--PQDFSS 570
           MQ QTHS FPGGN+QP  QQFPPSIDRSINQPWNASLSAN QGP SNPAAHA  PQDFSS
Sbjct: 481 MQSQTHSLFPGGNSQPGSQQFPPSIDRSINQPWNASLSANSQGPLSNPAAHAPPPQDFSS 540

Query: 571 PAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNS 630
           PAQG ASGVPQTSA EVKP+GRKELPADLF LNYSSYPAPVPGWH++PPRPMGYVMPYNS
Sbjct: 541 PAQGTASGVPQTSALEVKPSGRKELPADLFALNYSSYPAPVPGWHTIPPRPMGYVMPYNS 600

Query: 631 AMPVPSFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIP 690
            MP+PSFPQ S STNPFDLSSEQSQFPSMAPLQNA PSGQP SNL  TPGYGNPAS WIP
Sbjct: 601 PMPMPSFPQPSISTNPFDLSSEQSQFPSMAPLQNALPSGQPGSNLVRTPGYGNPASAWIP 660

Query: 691 HQPPTNLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQ 750
            QPP+NLS LPPQASYPSTM SRPPYMGQQVASNVQPYRHPGLGNYGNEG  HGYTDMSQ
Sbjct: 661 QQPPSNLSHLPPQASYPSTMHSRPPYMGQQVASNVQPYRHPGLGNYGNEGVAHGYTDMSQ 718

Query: 751 QTAGRFSAPATPNPFSSTGGNPFG 768
           QTAGRF APATPNPFSSTGGNPFG
Sbjct: 721 QTAGRFPAPATPNPFSSTGGNPFG 718

BLAST of Spg003475 vs. NCBI nr
Match: XP_038890016.1 (probable ADP-ribosylation factor GTPase-activating protein AGD14 isoform X1 [Benincasa hispida])

HSP 1 Score: 1246.1 bits (3223), Expect = 0.0e+00
Identity = 636/739 (86.06%), Postives = 662/739 (89.58%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDS-SNVMRLRDFIKHVYVD 150
           VKSISMAKFTSQEV ALQEGGNQRAKEIYFKELDPQRHSFPDS SNV+RLRDFIKHVYVD
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAKEIYFKELDPQRHSFPDSRSNVVRLRDFIKHVYVD 120

Query: 151 RRYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGG 210
           RRYSGDKNFDKPPRVK                          GEKED+YE RRTD+Y GG
Sbjct: 121 RRYSGDKNFDKPPRVK-------------------------SGEKEDFYETRRTDTYAGG 180

Query: 211 SRSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFG 270
           SRSPPY+DRRYNE+SSPGGRNFDERRSPGSDHEN QFGDFR+SPAR +VVNDWRREDRF 
Sbjct: 181 SRSPPYEDRRYNERSSPGGRNFDERRSPGSDHENRQFGDFRKSPARSEVVNDWRREDRFE 240

Query: 271 NGKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSG 330
           NGKRVEDGRLSDG+SKIGGRSPERPNDLDA+SPP VRPVRDILGEN+SPLRVIEPPKSSG
Sbjct: 241 NGKRVEDGRLSDGDSKIGGRSPERPNDLDASSPPTVRPVRDILGENVSPLRVIEPPKSSG 300

Query: 331 TSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSA 390
           TSKV D+SVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEP+PITSAV QPPQ+SA
Sbjct: 301 TSKVPDSSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPEPITSAVPQPPQSSA 360

Query: 391 PQSVTHPVNSNNDNNWASFDVPSQAPPAPANVGTLESVLSQLSVSASVPGVSGSHGATGA 450
           PQ +TH VNS +DNNWASFDV  QAPPAPANVGTLESVLSQLSVS SVPGVSG H A GA
Sbjct: 361 PQPITHTVNSTSDNNWASFDVAPQAPPAPANVGTLESVLSQLSVSGSVPGVSGLHAAAGA 420

Query: 451 VPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPNMQLQ 510
           +P APVGNMT+L TGFDPSFGSGGNTHMPPPF+GGAPSAAPGAGLSTFPPSGQW NMQ Q
Sbjct: 421 IPNAPVGNMTILPTGFDPSFGSGGNTHMPPPFSGGAPSAAPGAGLSTFPPSGQWSNMQSQ 480

Query: 511 THSSFPGGNTQPVGQQFPPSIDRSINQ-PWNASLSANPQGPQSNPAAHAPQDFSSPAQGI 570
           THS FPGGN+QPVGQQFPPSIDRSIN  PWNA  S N QGP SNPAAHAPQDFS+P+Q I
Sbjct: 481 THSLFPGGNSQPVGQQFPPSIDRSINHAPWNA--SQNSQGPLSNPAAHAPQDFSTPSQAI 540

Query: 571 ASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNSAMPVP 630
            SGVPQTSAPEVKP+GRKELPADLFTLNYSSYPAPVPGWH+VPPRPM Y MPYNSAMPVP
Sbjct: 541 TSGVPQTSAPEVKPSGRKELPADLFTLNYSSYPAPVPGWHTVPPRPMVYAMPYNSAMPVP 600

Query: 631 SFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIPHQPPT 690
           SFPQSSTSTNPFDLSSEQ QFPSMA LQNA PSGQP SNL H PGYGNP STW+PHQPP+
Sbjct: 601 SFPQSSTSTNPFDLSSEQPQFPSMASLQNALPSGQPTSNLIHNPGYGNPTSTWMPHQPPS 660

Query: 691 NLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQQTAGR 750
           NL QLPPQ  YPSTMPSRPPYMGQ VASNVQPYRHPGL NYGNEGA + YTDMSQQT GR
Sbjct: 661 NLLQLPPQGPYPSTMPSRPPYMGQHVASNVQPYRHPGLSNYGNEGAANAYTDMSQQTGGR 712

Query: 751 FSAPATPNPFSSTGGNPFG 768
           FSAPA PNPFSSTGGNPFG
Sbjct: 721 FSAPADPNPFSSTGGNPFG 712

BLAST of Spg003475 vs. ExPASy Swiss-Prot
Match: Q8RXE7 (Probable ADP-ribosylation factor GTPase-activating protein AGD14 OS=Arabidopsis thaliana OX=3702 GN=AGD14 PE=1 SV=2)

HSP 1 Score: 163.7 bits (413), Expect = 8.3e-39
Identity = 211/722 (29.22%), Postives = 296/722 (41.00%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           M    +E+E+NE+IIRGL+KL  NRRCINCNSLGPQYVCT FWTFVC  CSGIHREFTHR
Sbjct: 1   MMGSKREEERNEKIIRGLMKLPPNRRCINCNSLGPQYVCTTFWTFVCMACSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKS+SM+KFTS+EV  LQ GGNQRA+EIY K  D QR   P++SN  R+R+FIK+VYV +
Sbjct: 61  VKSVSMSKFTSKEVEVLQNGGNQRAREIYLKNWDHQRQRLPENSNAERVREFIKNVYVQK 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           +Y+G  + DKP +                         +S+         RR +SY   S
Sbjct: 121 KYAGANDADKPSK-------------------------DSQDHVSSEDMTRRANSYHSYS 180

Query: 211 RSPPYD----DRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRRED 270
           +SPPYD    +RRY +   P G      +S      + +   F  SP R    +D   ED
Sbjct: 181 QSPPYDYQYEERRYGK--IPLGFT---GKSASVKGLHAKASSFVYSPGR---FSDHMFED 240

Query: 271 RFGN---GKRVEDGRLSDGESKIGG--RSPERPNDLDAASPPMVRPVRDILGENISPLRV 330
           +F N     R  D  +S          +SP    + +  SP            N  P   
Sbjct: 241 QFSNEDSAPRASDYSVSSAGDPFRSDIQSPNFQQEAEFRSPQFQH-------SNAPPSEN 300

Query: 331 IEPPKSSGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAV 390
           + P +              QRT SS S+ S+  N +  K   SG L +  +E    T + 
Sbjct: 301 LFPGR------------QHQRTTSSGSVRSVDSNFMSIKSYTSGGLGEAVSESRQNTGSQ 360

Query: 391 TQPPQNSAP---QSVTHPVNSNNDNNWASFDVPSQAPPAPANVGTLESVLSQLSVSASVP 450
                N  P   +S   P++         F +P    P   +V T +  ++  S   ++ 
Sbjct: 361 QGKTSNHVPLVAESTKAPID--------LFQLP--GAPVAQSVDTFQPSIAPRSPPVNLQ 420

Query: 451 GVSGSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFP 510
               ++  T A   A  GN+    T   PS  S         F    P AA    + T P
Sbjct: 421 QAPQTYSFTPANSFA--GNLGQQPTS-RPSELSAPKNEGWASFDNPMP-AAKSTNVITSP 480

Query: 511 PSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSIDR---SINQPWNASLS------------ 570
              Q   ++L+         +  +   +P ++D+   SI  PW   LS            
Sbjct: 481 GDFQ---LELKIEEILQPSTSMQL-PPYPSTVDQHALSIPSPWQEDLSNVLKDVVDNPQP 540

Query: 571 --ANPQGPQSNPAAHAPQDFSSPAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYP 630
             A P   ++NP   + ++      G ++    T    ++    +EL  D       +  
Sbjct: 541 WNAFPDSIEANP-LDSSRNIHQQVDGASTSSYNTDHQHLESQVLEELSND------GTQT 600

Query: 631 APVPGWHSVPPRPMGYVMPYNSAMPVPSFPQSSTSTNPFDLS-----SEQSQFPSMAPLQ 690
             +P   S    P    M  + +           S NPFDL           F  M+ LQ
Sbjct: 601 TRIPAGSSAFGFPGNIGMAPSYSEEAWQHVNEQKSANPFDLPYDSEFDSNDMFLDMSSLQ 633

Query: 691 NAFPSGQPASNLTHTP-GYGNPASTWIPHQPPTNLSQLPPQASYPSTMPSRPPYMGQQVA 718
            A P  Q       TP  + N  S     QP      +P     P+       YM  Q +
Sbjct: 661 GALPDIQ-------TPQAFLNGVS-----QPWLAADSVPSYLPAPAVAQGGLAYMAGQAS 633

BLAST of Spg003475 vs. ExPASy Swiss-Prot
Match: Q2TA45 (Arf-GAP domain and FG repeat-containing protein 1 OS=Bos taurus OX=9913 GN=AGFG1 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 2.1e-10
Identity = 154/626 (24.60%), Postives = 237/626 (37.86%), Query Frame = 0

Query: 36  KEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFT--HRVKS 95
           K++EK+ +++R +  L  NR+C +C+  GP YV     +FVCT+CSG  R     HRVKS
Sbjct: 8   KQEEKHLKMLRDMTGLPHNRKCFDCDQRGPTYVNMTVGSFVCTSCSGSLRGLNPPHRVKS 67

Query: 96  ISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDRRYS 155
           ISM  FT QE+  LQ+ GN+  K+I+    D +  + PD  +  ++++F++  Y  +R+ 
Sbjct: 68  ISMTTFTQQEIEFLQKHGNEVCKQIWLGLFDDRSSAIPDFRDPQKVKEFLQEKYEKKRW- 127

Query: 156 GDKNFDKPPRVKPVRCP----KFFSANFSKMYLKLKAVMNSEGEKED-YYENRRTDSYQ- 215
               +  P + K V          SA+ +    ++K + +  G+     + N+ T S   
Sbjct: 128 ----YVPPEQAKVVASVHASISGSSASSTSSTPEVKPLKSLLGDSAPALHLNKGTPSQSP 187

Query: 216 --GGSRSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENW-QFGDFRRSPARPDVVNDWRR 275
             G S++   + ++++  S  G   F       +   N+  F  F    A+     D+  
Sbjct: 188 VVGRSQAQQQEKKQFDLLSDLGSDIFAAPAPQSTATANFANFAHFNSHAAQNSANADFAN 247

Query: 276 EDRFGNGKRVEDGRLSDGESKIGG-----RSPERPNDLDAASPPMVRPVRDILGENISPL 335
            D FG          S G S  GG      S  +P     ++  +          N +  
Sbjct: 248 FDAFGQ---------SSGSSNFGGFPTASHSSFQPQTTGGSAGSV----------NANFA 307

Query: 336 RVIEPPKSSGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITS 395
                PKSS        +    +TAS+ S  S ++  ++T  +   +L    A  D I S
Sbjct: 308 HFDNFPKSSSADFGTFNTSQSHQTASAVSKVSANKAGLQT-TDKYAAL----ANLDNIFS 367

Query: 396 AVTQPPQNSA-PQSVTHPVNSNNDNNWASFDVPSQAPPAP---ANVGTLESVLSQLSVSA 455
           A     Q S    +   PV S          VPSQ+  +    A +  L+SV S  + S+
Sbjct: 368 AGQGGDQGSGFGTTGKAPVGS-------VVSVPSQSSASSDKYAALAELDSVFSSAATSS 427

Query: 456 SVPGVSGSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPF-AGGAPSAAPGAGL 515
           +    S S+ ++    T PVG     Q          G T    PF A   PS A     
Sbjct: 428 NA-YTSTSNASSNVFGTVPVGASAQTQPASSSVPAPFGATPSTNPFVAAAGPSVASSTNP 487

Query: 516 STFPPSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNPA 575
                 G           S P G   P     P S   S  QP   + +A PQ       
Sbjct: 488 FQTNARGATAATFGTASMSMPAGFGTPAPYSLPTSFSGSFQQPAFPAQAAFPQ------- 547

Query: 576 AHAPQDFSSPAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRP 635
                 FS    G        + P V P G+      +     SS               
Sbjct: 548 ---QTAFSQQPNGAGFAAFGQTKPVVTPFGQ------VGAAGVSS--------------- 561

Query: 636 MGYVMPYNSAMPVPSFPQSSTSTNPF 641
                P+ +  P   FP  S+STNPF
Sbjct: 608 ----NPFMTGAPTGQFPTGSSSTNPF 561

BLAST of Spg003475 vs. ExPASy Swiss-Prot
Match: Q4KLH5 (Arf-GAP domain and FG repeat-containing protein 1 OS=Rattus norvegicus OX=10116 GN=Agfg1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.8e-10
Identity = 152/625 (24.32%), Postives = 236/625 (37.76%), Query Frame = 0

Query: 36  KEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFT--HRVKS 95
           K++EK+ +++R +  L  NR+C +C+  GP YV     +FVCT+CSG  R     HRVKS
Sbjct: 8   KQEEKHLKMLRDMTGLPHNRKCFDCDQRGPTYVNMTVGSFVCTSCSGSLRGLNPPHRVKS 67

Query: 96  ISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDRRYS 155
           ISM  FT QE+  LQ+ GN+  K+I+    D +  + PD  +  ++++F++  Y  +R+ 
Sbjct: 68  ISMTTFTQQEIEFLQKHGNEVCKQIWLGLFDDRSSAIPDFRDPQKVKEFLQEKYEKKRW- 127

Query: 156 GDKNFDKPPRVKPVRCP----KFFSANFSKMYLKLKAVMNSEGEKED-YYENRRTDSYQ- 215
               +  P + K V          SA+ +    ++K + +  GE     + N+ T +   
Sbjct: 128 ----YVPPEQAKVVASVHASISGSSASSTSSTPEVKPLKSLLGESAPALHLNKGTPTQSP 187

Query: 216 --GGSRSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENW-QFGDFRRSPARPDVVNDWRR 275
             G S+    + ++++  S  G   F       +   N+  F  F    A+     ++  
Sbjct: 188 VVGRSQGQQQEKKQFDLLSDLGSDIFAAPAPQSTATANFANFAHFNSHAAQNSANAEFAN 247

Query: 276 EDRFGNGKRVEDGRLSDGESKIGG-----RSPERPNDLDAASPPMVRPVRDILGENISPL 335
            D FG          S G S  GG      SP +P     ++  +          N +  
Sbjct: 248 FDAFGQ---------SSGSSNFGGFPTASHSPFQPQTTGGSAGSV----------NANFA 307

Query: 336 RVIEPPKSSGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITS 395
                PKSS     + ++    +TAS+ S  S ++  ++T  +   +L    A  D I S
Sbjct: 308 HFDNFPKSSSADFGSFSTSQSHQTASTVSKVSTNKAGLQT-ADKYAAL----ANLDNIFS 367

Query: 396 AVTQPPQNSA-PQSVTHPVNSNNDNNWASFDVPSQAPPAP---ANVGTLESVLSQLSVSA 455
           A     Q S    +   PV S          VPS +  +    A +  L+SV S  + S 
Sbjct: 368 AGQGGDQGSGFGTTGKAPVGS-------VVSVPSHSSASSDKYAALAELDSVFSSAATSN 427

Query: 456 SVPGVSGSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLS 515
           +    S S+ ++    T PVG     Q         G      P  A   PSAA      
Sbjct: 428 NA-YTSTSNASSSVFGTVPVGASPQTQPASSGPAPFGATPSTNPFVAATGPSAASSTNPF 487

Query: 516 TFPPSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNPAA 575
                G           S P G   P     P S   S  QP   + +A PQ        
Sbjct: 488 QTNARGATAATFGTASMSMPAGFGTPAQYSLPTSFSGSFQQPPFPAQAAFPQ-------- 547

Query: 576 HAPQDFSSPAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPM 635
                FS    G        + P V P G+      +     SS                
Sbjct: 548 --QTAFSQQPNGAGFATFGQTKPVVTPFGQ------VAAAGVSS---------------- 560

Query: 636 GYVMPYNSAMPVPSFPQSSTSTNPF 641
               P+ +  P   FP  S+STNPF
Sbjct: 608 ---NPFMTGAPTGQFPTGSSSTNPF 560

BLAST of Spg003475 vs. ExPASy Swiss-Prot
Match: P52594 (Arf-GAP domain and FG repeat-containing protein 1 OS=Homo sapiens OX=9606 GN=AGFG1 PE=1 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 3.6e-10
Identity = 153/627 (24.40%), Postives = 235/627 (37.48%), Query Frame = 0

Query: 36  KEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFT--HRVKS 95
           K++EK+ +++R +  L  NR+C +C+  GP YV     +FVCT+CSG  R     HRVKS
Sbjct: 8   KQEEKHLKMLRDMTGLPHNRKCFDCDQRGPTYVNMTVGSFVCTSCSGSLRGLNPPHRVKS 67

Query: 96  ISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDRRYS 155
           ISM  FT QE+  LQ+ GN+  K+I+    D +  + PD  +  ++++F++  Y  +R+ 
Sbjct: 68  ISMTTFTQQEIEFLQKHGNEVCKQIWLGLFDDRSSAIPDFRDPQKVKEFLQEKYEKKRW- 127

Query: 156 GDKNFDKPPRVKPVRCP----KFFSANFSKMYLKLKAVMNSEGEK-EDYYENRRTDSYQ- 215
               +  P + K V          SA+ +    ++K + +  G+     + N+ T S   
Sbjct: 128 ----YVPPEQAKVVASVHASISGSSASSTSSTPEVKPLKSLLGDSAPTLHLNKGTPSQSP 187

Query: 216 --GGSRSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENW-QFGDFRRSPARPDVVNDWRR 275
             G S+    + ++++  S  G   F       +   N+  F  F    A+     D+  
Sbjct: 188 VVGRSQGQQQEKKQFDLLSDLGSDIFAAPAPQSTATANFANFAHFNSHAAQNSANADFAN 247

Query: 276 EDRFGNGKRVEDGRLSDGESKIGG-----RSPERPNDLDAASPPMVRPVRDILGENISPL 335
            D FG          S G S  GG      SP +P     ++  +          N +  
Sbjct: 248 FDAFGQ---------SSGSSNFGGFPTASHSPFQPQTTGGSAASV----------NANFA 307

Query: 336 RVIEPPKSSGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITS 395
                PKSS        +    +TAS+ S  S ++  ++T  +   +L    A  D I S
Sbjct: 308 HFDNFPKSSSADFGTFNTSQSHQTASAVSKVSTNKAGLQT-ADKYAAL----ANLDNIFS 367

Query: 396 AVTQPPQNSA-PQSVTHPVNSNNDNNWASFDVPSQAPPAP---ANVGTLESVLSQLSVS- 455
           A     Q S    +   PV S          VPSQ+  +    A +  L+SV S  + S 
Sbjct: 368 AGQGGDQGSGFGTTGKAPVGS-------VVSVPSQSSASSDKYAALAELDSVFSSAATSS 427

Query: 456 -ASVPGVSGSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAG 515
            A     + S    G VP           +     FG+  +T+  P  A   PS A    
Sbjct: 428 NAYTSTSNASSNVFGTVPVVASAQTQPASSSVPAPFGATPSTN--PFVAAAGPSVASSTN 487

Query: 516 LSTFPPSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNP 575
                  G           S P G   P     P S   S  QP   + +A PQ      
Sbjct: 488 PFQTNARGATAATFGTASMSMPTGFGTPAPYSLPTSFSGSFQQPAFPAQAAFPQ------ 547

Query: 576 AAHAPQDFSSPAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPR 635
                  FS    G        + P V P G+      +     SS              
Sbjct: 548 ----QTAFSQQPNGAGFAAFGQTKPVVTPFGQ------VAAAGVSS-------------- 561

Query: 636 PMGYVMPYNSAMPVPSFPQSSTSTNPF 641
                 P+ +  P   FP  S+STNPF
Sbjct: 608 -----NPFMTGAPTGQFPTGSSSTNPF 561

BLAST of Spg003475 vs. ExPASy Swiss-Prot
Match: Q8K2K6 (Arf-GAP domain and FG repeat-containing protein 1 OS=Mus musculus OX=10090 GN=Agfg1 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 8.1e-10
Identity = 151/585 (25.81%), Postives = 231/585 (39.49%), Query Frame = 0

Query: 36  KEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFT--HRVKS 95
           K++EK+ +++R +  L  NR+C +C+  GP YV     +FVCT+CSG  R     HRVKS
Sbjct: 8   KQEEKHLKMLRDMTGLPHNRKCFDCDQRGPTYVNMTVGSFVCTSCSGSLRGLNPPHRVKS 67

Query: 96  ISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDRRYS 155
           ISM  FT QE+  LQ+ GN+  K+I+    D +  + PD  +  ++++F++  Y  +R+ 
Sbjct: 68  ISMTTFTQQEIEFLQKHGNEVCKQIWLGLFDDRSSAIPDFRDPQKVKEFLQEKYEKKRW- 127

Query: 156 GDKNFDKPPRVKPVRCP----KFFSANFSKMYLKLKAVMNSEGEKED-YYENRRTDSYQ- 215
               +  P + K V          SA+ +    ++K + +  GE     + N+ T S   
Sbjct: 128 ----YVPPEQAKVVASVHASISGSSASSTSSTPEVKPLKSLLGESAPALHLNKGTPSQSP 187

Query: 216 --GGSRSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENW-QFGDFRRSPARPDVVNDWRR 275
             G S+    + ++++  S  G   F       +   N+  F  F    A+     D+  
Sbjct: 188 VVGRSQGQQQEKKQFDLLSDLGSDIFAAPAPQSTATANFANFAHFNSHAAQNSANADFAN 247

Query: 276 EDRFGNGKRVEDGRLSDGESKIGG-----RSPERPNDLDAASPPMVRPVRDILGENISPL 335
            D FG          S G S  GG      S  +P     ++  +          N +  
Sbjct: 248 FDAFGQ---------SSGSSNFGGFPTASHSSFQPQTTGGSAGSV----------NANFA 307

Query: 336 RVIEPPKSSGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITS 395
                PKSS       ++    +TAS+ S  S ++  ++T  +   +L    A  D I S
Sbjct: 308 HFDNFPKSSSADFGTFSTSQSHQTASTVSKVSTNKAGLQT-ADKYAAL----ANLDNIFS 367

Query: 396 AVTQPPQNSA-PQSVTHPVNSNNDNNWASFDVPSQAPPAP---ANVGTLESVLSQLSVS- 455
           A     Q S    +   PV S          VPS +  +    A +  L+SV S  + S 
Sbjct: 368 AGQGGDQGSGFGTTGKAPVGS-------VVSVPSHSSASSDKYAALAELDSVFSSAATSS 427

Query: 456 -ASVPGVSGSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAG 515
            A  P  + S    G VP           +G  P FG+  +T+  P  A   PSAA    
Sbjct: 428 NAYTPTSNASSSVFGTVPVGASAQTQPASSGPAP-FGATPSTN--PFVAATGPSAASSTN 487

Query: 516 LSTFPPSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQ----GP 575
                  G           S P G   P     P S   S  QP   + +A PQ      
Sbjct: 488 PFQTNARGATAATFGTASMSMPAGFGTPAQYSLPTSFSGSFQQPAFPAQAAFPQQTAFSQ 547

Query: 576 QSNPAAHAPQDFSSP---------AQGIASGVPQTSAPEVK-PTG 585
           Q N A  A    + P         A G++S    T AP  + PTG
Sbjct: 548 QPNGAGFATFGQTKPVVTPFGQVAAAGVSSNPFMTGAPTGQLPTG 553

BLAST of Spg003475 vs. ExPASy TrEMBL
Match: A0A6J1HM50 (probable ADP-ribosylation factor GTPase-activating protein AGD14 OS=Cucurbita maxima OX=3661 GN=LOC111465450 PE=4 SV=1)

HSP 1 Score: 1247.3 bits (3226), Expect = 0.0e+00
Identity = 641/744 (86.16%), Postives = 665/744 (89.38%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRA+EIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAREIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFDKPPRVK                          GEKEDY ENRRTD+Y+GGS
Sbjct: 121 RYSGDKNFDKPPRVK-------------------------SGEKEDYNENRRTDTYRGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DRRYNE+SSPGGRNFD RRSPGSDHEN QFGDF  SP+RP+VVNDWRREDRFGN
Sbjct: 181 RSPPYEDRRYNERSSPGGRNFDGRRSPGSDHENRQFGDF-MSPSRPEVVNDWRREDRFGN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVED R SDG+SKIGGRSPE+PNDLDA+SPPMVRPVRDILGEN+SPLRVIEPPK SG 
Sbjct: 241 GKRVEDARSSDGDSKIGGRSPEQPNDLDASSPPMVRPVRDILGENVSPLRVIEPPKISGG 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           SK AD+SVHKQRTASSSSLGSISE+VVETKLEPSGSLIDFDAEP+PI SAV QPPQ+SAP
Sbjct: 301 SKAADSSVHKQRTASSSSLGSISESVVETKLEPSGSLIDFDAEPEPIASAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAP-----PAPANVGTLESVLSQLSVSASVPGVSGSHG 450
           QSVTH VN+N+DNNWASFDVP   P     PAPANVGTLESVLSQLSVSASVPGVSGSHG
Sbjct: 361 QSVTHAVNTNSDNNWASFDVPQAPPAPASAPAPANVGTLESVLSQLSVSASVPGVSGSHG 420

Query: 451 ATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPN 510
           A GAVPTAPV NMTML +GFDPSFGSGGNT MPPPFAGGAPSAAPG GLSTFPPSGQW N
Sbjct: 421 AVGAVPTAPVSNMTMLPSGFDPSFGSGGNTLMPPPFAGGAPSAAPGTGLSTFPPSGQWSN 480

Query: 511 MQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNPAAHA--PQDFSS 570
           MQ QTHS FPGGN+QP  QQFPPSIDRSINQPWNASLSAN QGP SNPAAHA  PQDFSS
Sbjct: 481 MQSQTHSLFPGGNSQPGSQQFPPSIDRSINQPWNASLSANSQGPLSNPAAHAPPPQDFSS 540

Query: 571 PAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNS 630
           PAQG ASGVPQTSA EVKP+GRKELPADLF LNYSSYPAPVPGWH++PPRPMGYVMPYNS
Sbjct: 541 PAQGTASGVPQTSALEVKPSGRKELPADLFALNYSSYPAPVPGWHTIPPRPMGYVMPYNS 600

Query: 631 AMPVPSFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIP 690
            MP+PSFPQ S STNPFDLSSEQSQFPSMAPLQNA PSGQP SNL  TPGYGNPAS WIP
Sbjct: 601 PMPMPSFPQPSISTNPFDLSSEQSQFPSMAPLQNALPSGQPGSNLVRTPGYGNPASAWIP 660

Query: 691 HQPPTNLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQ 750
            QPP+NLS LPPQASYPSTM SRPPYMGQQVASNVQPYRHPGLGNYGNEG  HGYTDMSQ
Sbjct: 661 QQPPSNLSHLPPQASYPSTMHSRPPYMGQQVASNVQPYRHPGLGNYGNEGVAHGYTDMSQ 718

Query: 751 QTAGRFSAPATPNPFSSTGGNPFG 768
           QTAGRF APATPNPFSSTGGNPFG
Sbjct: 721 QTAGRFPAPATPNPFSSTGGNPFG 718

BLAST of Spg003475 vs. ExPASy TrEMBL
Match: A0A6J1FB64 (probable ADP-ribosylation factor GTPase-activating protein AGD14 OS=Cucurbita moschata OX=3662 GN=LOC111443849 PE=4 SV=1)

HSP 1 Score: 1239.2 bits (3205), Expect = 0.0e+00
Identity = 640/744 (86.02%), Postives = 662/744 (88.98%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRA+EIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAREIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFDKPPRVK                          GEKEDY ENRRTD+Y+GGS
Sbjct: 121 RYSGDKNFDKPPRVK-------------------------SGEKEDYNENRRTDTYRGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DRRYNE+SSPGGRNFD RRSPGSDHEN QFGDF  SP RP+VVNDWRREDRFGN
Sbjct: 181 RSPPYEDRRYNERSSPGGRNFDGRRSPGSDHENRQFGDF-MSP-RPEVVNDWRREDRFGN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVED R SDG+SKIGGRSPE+PNDLDA+SPPMVRPVRDILGEN+SPLRVIEPPK SG 
Sbjct: 241 GKRVEDARSSDGDSKIGGRSPEQPNDLDASSPPMVRPVRDILGENVSPLRVIEPPKISGG 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           SK AD+SVHKQRTASSSSLGSISE+VVETKLEPSGSLIDFDAEP+PI SAV QPPQ+SAP
Sbjct: 301 SKAADSSVHKQRTASSSSLGSISESVVETKLEPSGSLIDFDAEPEPIASAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAP-----PAPANVGTLESVLSQLSVSASVPGVSGSHG 450
           QSVT+ VNSNNDNNWASFDVP   P     PAPANVGTLESVLSQLSVSASVPG+SGSHG
Sbjct: 361 QSVTYAVNSNNDNNWASFDVPQAPPAPAPAPAPANVGTLESVLSQLSVSASVPGISGSHG 420

Query: 451 ATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPN 510
           A GAVPTAPV NM ML +GFDPSFGSGGNT MPPPFAGGAPSAAPG GLSTFPPSGQW N
Sbjct: 421 AVGAVPTAPVSNMMMLPSGFDPSFGSGGNTLMPPPFAGGAPSAAPGTGLSTFPPSGQWSN 480

Query: 511 MQLQTHSSFPGGNTQPVGQQFPPSIDRSINQPWNASLSANPQGPQSNPAAH--APQDFSS 570
           MQ QTHS FPGGN+QP GQQFPPSIDRSINQPWNASLSAN QGP SNPAAH  APQDFSS
Sbjct: 481 MQSQTHSLFPGGNSQPGGQQFPPSIDRSINQPWNASLSANSQGPLSNPAAHAPAPQDFSS 540

Query: 571 PAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNS 630
           PAQG ASGVPQTSAPEVKP+GRKELPADLF LNYSSYPAPVPGWH+VPPRPMGYVMPYNS
Sbjct: 541 PAQGTASGVPQTSAPEVKPSGRKELPADLFALNYSSYPAPVPGWHTVPPRPMGYVMPYNS 600

Query: 631 AMPVPSFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIP 690
            MP+PSFPQ S STNPFDLSSEQSQFPSMAPLQNA PSGQP SNL  TPGYGNPAS WIP
Sbjct: 601 PMPMPSFPQPSISTNPFDLSSEQSQFPSMAPLQNALPSGQPGSNLIRTPGYGNPASAWIP 660

Query: 691 HQPPTNLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQ 750
            QPP+NLS LPPQASYPSTM SRPPYM QQV SNVQPYRHPGLG YGNEG  HGYTDMSQ
Sbjct: 661 QQPPSNLSHLPPQASYPSTMHSRPPYMRQQVVSNVQPYRHPGLGTYGNEGVAHGYTDMSQ 717

Query: 751 QTAGRFSAPATPNPFSSTGGNPFG 768
           QTAGRF APATPNPFSSTGGNPFG
Sbjct: 721 QTAGRFPAPATPNPFSSTGGNPFG 717

BLAST of Spg003475 vs. ExPASy TrEMBL
Match: A0A0A0LKU9 (Arf-GAP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G005280 PE=4 SV=1)

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 627/738 (84.96%), Postives = 657/738 (89.02%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFD+PPRVK                          GEKED YE RR D+YQGGS
Sbjct: 121 RYSGDKNFDRPPRVK-------------------------SGEKEDTYETRRADTYQGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DRRYNE+SSPGGRNFDERRSPGSDHEN QFGDFR+SPAR +VVNDWRREDRFGN
Sbjct: 181 RSPPYEDRRYNERSSPGGRNFDERRSPGSDHENRQFGDFRKSPARSEVVNDWRREDRFGN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVEDGRLSDG+SKIGGRSP+RPNDLD +SPPMVRPVRDILGEN+SPLRVIEPPKSSGT
Sbjct: 241 GKRVEDGRLSDGDSKIGGRSPDRPNDLDVSSPPMVRPVRDILGENVSPLRVIEPPKSSGT 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           +KVAD+SVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEP+PI SAV QPPQ+SAP
Sbjct: 301 TKVADSSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPEPIASAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAPPAPANVGTLESVLSQLSVSASVPGVSGSHGATGAV 450
           Q VTHPVN+  DNNWASFDV   APPAPANVGTLESVLSQLSVS SVPGVSGSHGA GAV
Sbjct: 361 QPVTHPVNTTGDNNWASFDVTPHAPPAPANVGTLESVLSQLSVSGSVPGVSGSHGAAGAV 420

Query: 451 PTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPNMQLQT 510
           P APVG+MTML TGFDPSFGSGGN HM PPF+GGAPSA PGAGLSTFPPSGQW N+Q QT
Sbjct: 421 PNAPVGSMTMLPTGFDPSFGSGGNAHMSPPFSGGAPSAGPGAGLSTFPPSGQWSNVQSQT 480

Query: 511 HSSFPGGNTQPVGQQFPPSIDRSINQ-PWNASLSANPQGPQSNPAAHAPQDFSSPAQGIA 570
           HS FPGGN QP GQQFPPS+DR+IN  PWNA  S N QGP SNPAAHAPQDFS+P+Q + 
Sbjct: 481 HSLFPGGNPQPAGQQFPPSMDRTINHVPWNA--SHNSQGPLSNPAAHAPQDFSTPSQALP 540

Query: 571 SGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNSAMPVPS 630
           SGVPQTSAPEVKP+GRKELPADLFT NYSSYPAPVPGWH+VP RP+ Y MPYN AMPVPS
Sbjct: 541 SGVPQTSAPEVKPSGRKELPADLFTFNYSSYPAPVPGWHTVPQRPVVYAMPYNPAMPVPS 600

Query: 631 FPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIPHQPPTN 690
           FPQSSTSTNPFDLSSEQ QFPSM PLQN  PSGQPA NL H PGYGNP STW+PHQPP+N
Sbjct: 601 FPQSSTSTNPFDLSSEQPQFPSMGPLQNTLPSGQPAPNLIHNPGYGNPTSTWMPHQPPSN 660

Query: 691 LSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQQTAGRF 750
           LSQLPPQ  Y  TMPSRPPYMGQQ+ASNVQPYRHPGLGNYGNEGAT  YTDMSQQTAGRF
Sbjct: 661 LSQLPPQGPYQPTMPSRPPYMGQQIASNVQPYRHPGLGNYGNEGATGVYTDMSQQTAGRF 711

Query: 751 SAPATPNPFSSTGGNPFG 768
           SAP+TPNPFSSTGGNPFG
Sbjct: 721 SAPSTPNPFSSTGGNPFG 711

BLAST of Spg003475 vs. ExPASy TrEMBL
Match: A0A1S3C7A1 (probable ADP-ribosylation factor GTPase-activating protein AGD14 OS=Cucumis melo OX=3656 GN=LOC103497698 PE=4 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 630/739 (85.25%), Postives = 659/739 (89.17%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFD+PPRVK                          GEKED YE R+ D+YQGGS
Sbjct: 121 RYSGDKNFDRPPRVK-------------------------SGEKEDTYEIRKADTYQGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DRRYNE+SSPGGRNFDERRSPGSDHEN QFGDFR+SPAR +VVNDWRREDRFGN
Sbjct: 181 RSPPYEDRRYNERSSPGGRNFDERRSPGSDHENRQFGDFRKSPARSEVVNDWRREDRFGN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVEDGRLSDG+SKIGGRSP+RPNDLD +SPPMVRPVRDILGEN+SPLRVIEPPKSSGT
Sbjct: 241 GKRVEDGRLSDGDSKIGGRSPDRPNDLDVSSPPMVRPVRDILGENVSPLRVIEPPKSSGT 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           +KVAD SVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEP+PI SAV QPPQ+SAP
Sbjct: 301 TKVADGSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPEPIASAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAPPAPANVGTLESVLSQLSVSASVPGVSGSHG-ATGA 450
           Q +TH VNS +DNNWASFDV   APPAPANVGTLESVLSQLSVS SVPGVSGSHG A GA
Sbjct: 361 QPITHTVNSTSDNNWASFDVTPHAPPAPANVGTLESVLSQLSVSGSVPGVSGSHGAAAGA 420

Query: 451 VPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPNMQLQ 510
           VP APVGNMTML TGFDPSFGSGGN HM PPF+GGAPSA PG GLSTFPPSGQW N+Q Q
Sbjct: 421 VPNAPVGNMTMLPTGFDPSFGSGGNAHMSPPFSGGAPSAGPGTGLSTFPPSGQWSNVQSQ 480

Query: 511 THSSFPGGNTQPVGQQFPPSIDRSINQ-PWNASLSANPQGPQSNPAAHAPQDFSSPAQGI 570
           THS FPGGN QP GQQFPPS+DR+IN  PWNA  S N QGP SNPAAHAPQDFS+PAQ I
Sbjct: 481 THSLFPGGNPQPAGQQFPPSMDRTINHVPWNA--SHNSQGPLSNPAAHAPQDFSTPAQAI 540

Query: 571 ASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNSAMPVP 630
            SGVPQTSAPEVKP+GRKELPADLFTLNYSSYPAPVPGWH+VPPRPM Y MPYNSAMP+P
Sbjct: 541 PSGVPQTSAPEVKPSGRKELPADLFTLNYSSYPAPVPGWHTVPPRPMVYAMPYNSAMPMP 600

Query: 631 SFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIPHQPPT 690
           SFPQSSTSTNPFDLSSEQ QFPSMAPLQNA PSGQPA NL H PGYGNP STW+PHQPP+
Sbjct: 601 SFPQSSTSTNPFDLSSEQPQFPSMAPLQNALPSGQPAPNLIHNPGYGNPTSTWMPHQPPS 660

Query: 691 NLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQQTAGR 750
           N+ QLPPQ  Y  T+PSRPPYMGQQVASNVQPYRHPGLGNYGNEGAT  YTDMSQQTAGR
Sbjct: 661 NIPQLPPQGPYQPTLPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATSVYTDMSQQTAGR 712

Query: 751 FSAPATPNPFSSTGGNPFG 768
           F+APATPNPFSSTGGNPFG
Sbjct: 721 FTAPATPNPFSSTGGNPFG 712

BLAST of Spg003475 vs. ExPASy TrEMBL
Match: A0A5D3CJW4 (Putative ADP-ribosylation factor GTPase-activating protein AGD14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00150 PE=4 SV=1)

HSP 1 Score: 1233.4 bits (3190), Expect = 0.0e+00
Identity = 629/739 (85.12%), Postives = 658/739 (89.04%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR
Sbjct: 1   MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV ALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR
Sbjct: 61  VKSISMAKFTSQEVSALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RYSGDKNFD+PPRVK                          GEKED YE R+ D+YQGGS
Sbjct: 121 RYSGDKNFDRPPRVK-------------------------SGEKEDTYETRKADTYQGGS 180

Query: 211 RSPPYDDRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDRFGN 270
           RSPPY+DRRYNE+SSPGGRNFDERRSPGSDHEN QFGDFR+SPAR +VVNDWRREDRFGN
Sbjct: 181 RSPPYEDRRYNERSSPGGRNFDERRSPGSDHENRQFGDFRKSPARSEVVNDWRREDRFGN 240

Query: 271 GKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKSSGT 330
           GKRVEDGRLSDG+SKIGGRSP+RPNDLD +SPPMVRPVRDILGEN+SPLRVIEPPKSSGT
Sbjct: 241 GKRVEDGRLSDGDSKIGGRSPDRPNDLDVSSPPMVRPVRDILGENVSPLRVIEPPKSSGT 300

Query: 331 SKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPDPITSAVTQPPQNSAP 390
           +KVAD SVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEP+PI SAV QPPQ+SAP
Sbjct: 301 TKVADGSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAEPEPIASAVPQPPQSSAP 360

Query: 391 QSVTHPVNSNNDNNWASFDVPSQAPPAPANVGTLESVLSQLSVSASVPGVSGSHG-ATGA 450
           Q +TH VNS +DNNWASFDV   APPA ANVGTLESVLSQLSVS SVPGVSGSHG A GA
Sbjct: 361 QPITHTVNSTSDNNWASFDVTPHAPPAHANVGTLESVLSQLSVSGSVPGVSGSHGAAAGA 420

Query: 451 VPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSGQWPNMQLQ 510
           VP APVGNMTML TGFDPSFGSGGN HM PPF+GGAPSA PG GLSTFPPSGQW N+Q Q
Sbjct: 421 VPNAPVGNMTMLPTGFDPSFGSGGNAHMSPPFSGGAPSAGPGTGLSTFPPSGQWSNVQSQ 480

Query: 511 THSSFPGGNTQPVGQQFPPSIDRSINQ-PWNASLSANPQGPQSNPAAHAPQDFSSPAQGI 570
           THS FPGGN QP GQQFPPS+DR+IN  PWNA  S N QGP SNPAAHAPQDFS+PAQ I
Sbjct: 481 THSLFPGGNPQPAGQQFPPSMDRTINHVPWNA--SHNSQGPLSNPAAHAPQDFSTPAQAI 540

Query: 571 ASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVMPYNSAMPVP 630
            SGVPQTSAPEVKP+GRKELPADLFTLNYSSYPAPVPGWH+VPPRPM Y MPYNSAMP+P
Sbjct: 541 PSGVPQTSAPEVKPSGRKELPADLFTLNYSSYPAPVPGWHTVPPRPMVYAMPYNSAMPMP 600

Query: 631 SFPQSSTSTNPFDLSSEQSQFPSMAPLQNAFPSGQPASNLTHTPGYGNPASTWIPHQPPT 690
           SFPQSSTSTNPFDLSSEQ QFPSMAPLQNA PSGQPA NL H PGYGNP STW+PHQPP+
Sbjct: 601 SFPQSSTSTNPFDLSSEQPQFPSMAPLQNALPSGQPAPNLIHNPGYGNPTSTWMPHQPPS 660

Query: 691 NLSQLPPQASYPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATHGYTDMSQQTAGR 750
           N+ QLPPQ  Y  T+PSRPPYMGQQVASNVQPYRHPGLGNYGNEGAT  YTDMSQQTAGR
Sbjct: 661 NIPQLPPQGPYQPTLPSRPPYMGQQVASNVQPYRHPGLGNYGNEGATSVYTDMSQQTAGR 712

Query: 751 FSAPATPNPFSSTGGNPFG 768
           F+APATPNPFSSTGGNPFG
Sbjct: 721 FTAPATPNPFSSTGGNPFG 712

BLAST of Spg003475 vs. TAIR 10
Match: AT4G13350.1 (NSP (nuclear shuttle protein)-interacting GTPase )

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-127
Identity = 328/758 (43.27%), Postives = 397/758 (52.37%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MA RVKEDEKNE+IIR LLKL EN+RCINCNSLGPQYVCT FWTFVCT CSGIHREFTHR
Sbjct: 1   MAGRVKEDEKNEKIIRSLLKLPENKRCINCNSLGPQYVCTTFWTFVCTNCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV AL+EGGNQ AK+IYFK LD QR S PD SNV RLRDFI+HVYV++
Sbjct: 61  VKSISMAKFTSQEVTALKEGGNQHAKDIYFKGLDQQRQSVPDGSNVERLRDFIRHVYVNK 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RY+ +KN DK P                                    E R +     GS
Sbjct: 121 RYTNEKNDDKSPS-----------------------------------ETRSS----SGS 180

Query: 211 RSPPYD---DRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDR 270
           RSPPY+   DRRY ++SSPGG      RSPG +  +    + R+SPARP+++NDWRREDR
Sbjct: 181 RSPPYEDGYDRRYGDRSSPGG------RSPGFETGSRNAVNNRKSPARPEILNDWRREDR 240

Query: 271 FGNGKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKS 330
           FG  K  E+          G +SPE+  DL +ASPP+ RPVR+ILG+++ PLRV EPPK 
Sbjct: 241 FGGRKTSEE----------GSQSPEQVKDLGSASPPVARPVREILGDSVIPLRVGEPPKP 300

Query: 331 SGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAE-PDPITSAVTQPPQ 390
              S+  D S H +   S SSL S +E   E KLE + SLIDFD +   P  S   Q P 
Sbjct: 301 P-VSRNTDASAHAKSGTSLSSLMSTNEKPPEVKLETALSLIDFDTDFETPAPSVAIQAPL 360

Query: 391 NSAPQSVTHPVNSNNDNNWASFDVPSQAP-----PAPANVGTLESVLSQLSVSASVPGVS 450
           ++  Q    P  S+ND NWASFD    AP       P +  TL+S+LSQL+V++SVPG  
Sbjct: 361 STTSQPAPQPTTSSND-NWASFDAAPSAPSLNVSQPPPSGNTLDSLLSQLAVTSSVPG-- 420

Query: 451 GSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSG 510
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 511 QWPNMQLQTHSSFPGGNTQPVGQQFPP-SIDRSINQPWNASLSANPQGPQSNPAAHAPQD 570
                Q  T S+ P        Q F P   + S  QPWN +L++N Q   S P+      
Sbjct: 481 -----QASTPSNGPVNLGHSTSQIFAPFQNEHSSEQPWNTALASNVQRSMSAPSL----- 540

Query: 571 FSSPAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVM- 630
              P QG+ SG  Q+S  EVKP+GR ELPADLF +NY SY APVPGW + PP  M Y M 
Sbjct: 541 --QPLQGVPSGGLQSS--EVKPSGRSELPADLFAVNYPSYHAPVPGWQAGPPHAMHYGMQ 600

Query: 631 PYNSAMPVPSFPQSSTSTNPFDLS-------SEQSQFPSMAPLQNAFPSGQPASNLTHTP 690
            YN+ +P  + PQ   S NPFD S         ++ FPSMAPLQ A              
Sbjct: 601 QYNNPVPYQNVPQPGKSMNPFDFSPGPPSQTQTENMFPSMAPLQGAL------------- 602

Query: 691 GYGNPASTWIPHQPPTNLSQLPPQAS-YPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGN 750
               P S  +P Q   N   +P Q S +PS MP  P YM  Q+  ++ P     +G+   
Sbjct: 661 ----PPSGMMPSQGVHNQFNIPSQGSAHPSAMP--PRYMPSQIPGSMPPSNVNPIGDIN- 602

Query: 751 EGATHGYTDMSQQTAGRFSAPATP-NPFS-STGGNPFG 768
              T   T  + Q  G   A A P NP S  +GGNPFG
Sbjct: 721 ---TSYDTQQTYQNFGSSFAAAVPLNPPSFQSGGNPFG 602

BLAST of Spg003475 vs. TAIR 10
Match: AT4G13350.2 (NSP (nuclear shuttle protein)-interacting GTPase )

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-127
Identity = 328/758 (43.27%), Postives = 397/758 (52.37%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           MA RVKEDEKNE+IIR LLKL EN+RCINCNSLGPQYVCT FWTFVCT CSGIHREFTHR
Sbjct: 1   MAGRVKEDEKNEKIIRSLLKLPENKRCINCNSLGPQYVCTTFWTFVCTNCSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKSISMAKFTSQEV AL+EGGNQ AK+IYFK LD QR S PD SNV RLRDFI+HVYV++
Sbjct: 61  VKSISMAKFTSQEVTALKEGGNQHAKDIYFKGLDQQRQSVPDGSNVERLRDFIRHVYVNK 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           RY+ +KN DK P                                    E R +     GS
Sbjct: 121 RYTNEKNDDKSPS-----------------------------------ETRSS----SGS 180

Query: 211 RSPPYD---DRRYNEKSSPGGRNFDERRSPGSDHENWQFGDFRRSPARPDVVNDWRREDR 270
           RSPPY+   DRRY ++SSPGG      RSPG +  +    + R+SPARP+++NDWRREDR
Sbjct: 181 RSPPYEDGYDRRYGDRSSPGG------RSPGFETGSRNAVNNRKSPARPEILNDWRREDR 240

Query: 271 FGNGKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPPKS 330
           FG  K  E+          G +SPE+  DL +ASPP+ RPVR+ILG+++ PLRV EPPK 
Sbjct: 241 FGGRKTSEE----------GSQSPEQVKDLGSASPPVARPVREILGDSVIPLRVGEPPKP 300

Query: 331 SGTSKVADTSVHKQRTASSSSLGSISENVVETKLEPSGSLIDFDAE-PDPITSAVTQPPQ 390
              S+  D S H +   S SSL S +E   E KLE + SLIDFD +   P  S   Q P 
Sbjct: 301 P-VSRNTDASAHAKSGTSLSSLMSTNEKPPEVKLETALSLIDFDTDFETPAPSVAIQAPL 360

Query: 391 NSAPQSVTHPVNSNNDNNWASFDVPSQAP-----PAPANVGTLESVLSQLSVSASVPGVS 450
           ++  Q    P  S+ND NWASFD    AP       P +  TL+S+LSQL+V++SVPG  
Sbjct: 361 STTSQPAPQPTTSSND-NWASFDAAPSAPSLNVSQPPPSGNTLDSLLSQLAVTSSVPG-- 420

Query: 451 GSHGATGAVPTAPVGNMTMLQTGFDPSFGSGGNTHMPPPFAGGAPSAAPGAGLSTFPPSG 510
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 511 QWPNMQLQTHSSFPGGNTQPVGQQFPP-SIDRSINQPWNASLSANPQGPQSNPAAHAPQD 570
                Q  T S+ P        Q F P   + S  QPWN +L++N Q   S P+      
Sbjct: 481 -----QASTPSNGPVNLGHSTSQIFAPFQNEHSSEQPWNTALASNVQRSMSAPSL----- 540

Query: 571 FSSPAQGIASGVPQTSAPEVKPTGRKELPADLFTLNYSSYPAPVPGWHSVPPRPMGYVM- 630
              P QG+ SG  Q+S  EVKP+GR ELPADLF +NY SY APVPGW + PP  M Y M 
Sbjct: 541 --QPLQGVPSGGLQSS--EVKPSGRSELPADLFAVNYPSYHAPVPGWQAGPPHAMHYGMQ 600

Query: 631 PYNSAMPVPSFPQSSTSTNPFDLS-------SEQSQFPSMAPLQNAFPSGQPASNLTHTP 690
            YN+ +P  + PQ   S NPFD S         ++ FPSMAPLQ A              
Sbjct: 601 QYNNPVPYQNVPQPGKSMNPFDFSPGPPSQTQTENMFPSMAPLQGAL------------- 602

Query: 691 GYGNPASTWIPHQPPTNLSQLPPQAS-YPSTMPSRPPYMGQQVASNVQPYRHPGLGNYGN 750
               P S  +P Q   N   +P Q S +PS MP  P YM  Q+  ++ P     +G+   
Sbjct: 661 ----PPSGMMPSQGVHNQFNIPSQGSAHPSAMP--PRYMPSQIPGSMPPSNVNPIGDIN- 602

Query: 751 EGATHGYTDMSQQTAGRFSAPATP-NPFS-STGGNPFG 768
              T   T  + Q  G   A A P NP S  +GGNPFG
Sbjct: 721 ---TSYDTQQTYQNFGSSFAAAVPLNPPSFQSGGNPFG 602

BLAST of Spg003475 vs. TAIR 10
Match: AT1G08680.3 (ARF GAP-like zinc finger-containing protein ZIGA4 )

HSP 1 Score: 196.1 bits (497), Expect = 1.1e-49
Identity = 102/196 (52.04%), Postives = 127/196 (64.80%), Query Frame = 0

Query: 31  MANRVKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHR 90
           M    +E+E+NE+IIRGL+KL  NRRCINCNSLGPQYVCT FWTFVC  CSGIHREFTHR
Sbjct: 1   MMGSKREEERNEKIIRGLMKLPPNRRCINCNSLGPQYVCTTFWTFVCMACSGIHREFTHR 60

Query: 91  VKSISMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDR 150
           VKS+SM+KFTS+EV  LQ GGNQRA+EIY K  D QR   P++SN  R+R+FIK+VYV +
Sbjct: 61  VKSVSMSKFTSKEVEVLQNGGNQRAREIYLKNWDHQRQRLPENSNAERVREFIKNVYVQK 120

Query: 151 RYSGDKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGS 210
           +Y+G  + DKP +                         +S+         RR +SY   S
Sbjct: 121 KYAGANDADKPSK-------------------------DSQDHVSSEDMTRRANSYHSYS 171

Query: 211 RSPPYD----DRRYNE 223
           +SPPYD    +RRY +
Sbjct: 181 QSPPYDYQYEERRYGK 171

BLAST of Spg003475 vs. TAIR 10
Match: AT4G32630.2 (ArfGap/RecO-like zinc finger domain-containing protein )

HSP 1 Score: 181.8 bits (460), Expect = 2.1e-45
Identity = 216/700 (30.86%), Postives = 304/700 (43.43%), Query Frame = 0

Query: 35  VKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHRVKSI 94
           +KEDE+ E+ IR LLKL ENRRCINCNSLGPQYVC+ FWTFVC  CSGIHREFTHRVKS+
Sbjct: 1   MKEDERTEKAIRSLLKLPENRRCINCNSLGPQYVCSTFWTFVCVNCSGIHREFTHRVKSV 60

Query: 95  SMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDRRYSG 154
           SMAKFT+ EV AL+ GGN+RA++IYFKE D  R  +PD SN+ +LRDFI+ VYVD+RYS 
Sbjct: 61  SMAKFTADEVSALRAGGNERARQIYFKEWDAHRDGYPDRSNIFKLRDFIRSVYVDKRYSS 120

Query: 155 DKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGSRSPP 214
                                         K         EDY E+++T ++  GSRS  
Sbjct: 121 SD----------------------------KISQQKSDVTEDYRESKKTSAHVLGSRSLH 180

Query: 215 YDDRRYNEKSSPGGRN--------FDERRSPGSD-HENWQFGDFRRSPARPDVVNDWRRE 274
             D+   E+SS  GR+        FD++         N +     +SP R ++V+D  R+
Sbjct: 181 SVDKSDIERSSAAGRSGSESLRFYFDDKNHKQQHVTHNPRSRGLPKSPIRFEIVDDRFRD 240

Query: 275 DRFGNGKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPP 334
           D  G+ KR +  + S G SK    S +  ++ D  S P+VR        + S L +++  
Sbjct: 241 D--GSVKRYDARKDSRGSSK----SLDLSSNKDMPSFPIVR--------HTSELNIVKVE 300

Query: 335 KSSGTSKVADTSVHKQRTASSSSL---GSISENVVETKLEPSGSLIDFDAEPDPITSAVT 394
           K           V+ Q TASS  +    S+ ++V  ++L   G +I   +E         
Sbjct: 301 KKK-------DPVNNQMTASSEKMEIPRSLIDDVPVSELSDEG-IIKNSSEIPASLKTTE 360

Query: 395 QPPQNSAP-----QSVTHPV-NSNNDNNWASFDVPSQAPPAPANVG--TLESVLSQLSVS 454
           +P  NS        SV   V  +NN   W + D+ S       N+G  T+  +   ++  
Sbjct: 361 EPAPNSLEALLFGSSVPSVVPGTNNYELWNTSDISSTENYTAVNLGTQTMPGIPDSVTSF 420

Query: 455 ASVPGVSGSH-GATG-AVPTAP----------------VGNMTMLQTGF---DPSFGSGG 514
           A+ P  + +H G++G  VP AP                  + +M QT     D + G G 
Sbjct: 421 ATSPTTAHAHSGSSGPVVPVAPDNLNTKETATLANNQGPSDFSMEQTTLAITDYAHGVGS 480

Query: 515 NTHMPPPFAGGAPSAAPG---AGLSTFPPSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSI 574
             H           A P     G  +F P         Q H    G        Q+P   
Sbjct: 481 EHHQDDETQSSIRKALPEDLFTGGFSFAPQ--------QVHGQHHGMGYGMQYYQYPVQA 540

Query: 575 DRSINQPWNAS----LSANPQGPQSNPAAHAPQDFSSPAQGIASGVPQTSAPEVKPTGRK 634
             ++     A+    LS +   P   P       F + A     G+P  S+P +  +   
Sbjct: 541 MGALTYTAKAANPFDLSYDDTAPNQTP------QFPTMAYVQGGGLPHVSSP-IGYSDSS 600

Query: 635 ELPADLFTLNYSSYP---------APVPGWHSVPPRPMGYVMPYNSAMPVPSFPQSSTST 678
              AD   L  S  P         +P    H  P   MG     N +   PSF Q  +  
Sbjct: 601 SPAADSIGLMTSQSPFHATALSPNSPALASHLSPGALMGQQSQVNMS---PSFRQEYSG- 626

BLAST of Spg003475 vs. TAIR 10
Match: AT4G32630.1 (ArfGap/RecO-like zinc finger domain-containing protein )

HSP 1 Score: 180.6 bits (457), Expect = 4.6e-45
Identity = 216/699 (30.90%), Postives = 304/699 (43.49%), Query Frame = 0

Query: 35  VKEDEKNERIIRGLLKLQENRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHRVKSI 94
           +KEDE+ E+ IR LLKL ENRRCINCNSLGPQYVC+ FWTFVC  CSGIHREFTHRVKS+
Sbjct: 1   MKEDERTEKAIRSLLKLPENRRCINCNSLGPQYVCSTFWTFVCVNCSGIHREFTHRVKSV 60

Query: 95  SMAKFTSQEVIALQEGGNQRAKEIYFKELDPQRHSFPDSSNVMRLRDFIKHVYVDRRYSG 154
           SMAKFT+ EV AL+ GGN+RA++IYFKE D  R  +PD SN+ +LRDFI+ VYVD+RYS 
Sbjct: 61  SMAKFTADEVSALRAGGNERARQIYFKEWDAHRDGYPDRSNIFKLRDFIRSVYVDKRYSS 120

Query: 155 DKNFDKPPRVKPVRCPKFFSANFSKMYLKLKAVMNSEGEKEDYYENRRTDSYQGGSRSPP 214
                                         K         EDY E+++T ++  GSRS  
Sbjct: 121 SD----------------------------KISQQKSDVTEDYRESKKTSAHVLGSRSLH 180

Query: 215 YDDRRYNEKSSPGGRN--------FDERRSPGSD-HENWQFGDFRRSPARPDVVNDWRRE 274
             D+   E+SS  GR+        FD++         N +     +SP R ++V+D  R+
Sbjct: 181 SVDKSDIERSSAAGRSGSESLRFYFDDKNHKQQHVTHNPRSRGLPKSPIRFEIVDDRFRD 240

Query: 275 DRFGNGKRVEDGRLSDGESKIGGRSPERPNDLDAASPPMVRPVRDILGENISPLRVIEPP 334
           D  G+ KR +  + S G SK    S +  ++ D  S P+VR        + S L +++  
Sbjct: 241 D--GSVKRYDARKDSRGSSK----SLDLSSNKDMPSFPIVR--------HTSELNIVKVE 300

Query: 335 KSSGTSKVADTSVHKQRTASSSSL---GSISENVVETKLEPSGSLIDFDAEPDPITSAVT 394
           K           V+ Q TASS  +    S+ ++V  ++L   G +I   +E         
Sbjct: 301 KKK-------DPVNNQMTASSEKMEIPRSLIDDVPVSELSDEG-IIKNSSEIPASLKTTE 360

Query: 395 QPPQNSAP-----QSVTHPV-NSNNDNNWASFDVPSQAPPAPANVG--TLESVLSQLSVS 454
           +P  NS        SV   V  +NN   W + D+ S       N+G  T+  +   ++  
Sbjct: 361 EPAPNSLEALLFGSSVPSVVPGTNNYELWNTSDISSTENYTAVNLGTQTMPGIPDSVTSF 420

Query: 455 ASVPGVSGSH-GATG-AVPTAP----------------VGNMTMLQTGF---DPSFGSGG 514
           A+ P  + +H G++G  VP AP                  + +M QT     D + G G 
Sbjct: 421 ATSPTTAHAHSGSSGPVVPVAPDNLNTKETATLANNQGPSDFSMEQTTLAITDYAHGVGS 480

Query: 515 NTHMPPPFAGGAPSAAPG---AGLSTFPPSGQWPNMQLQTHSSFPGGNTQPVGQQFPPSI 574
             H           A P     G  +F P         Q H    G        Q+P ++
Sbjct: 481 EHHQDDETQSSIRKALPEDLFTGGFSFAPQ--------QVHGQHHGMGYGMQYYQYPVAM 540

Query: 575 DRSINQPWNAS---LSANPQGPQSNPAAHAPQDFSSPAQGIASGVPQTSAPEVKPTGRKE 634
                    A+   LS +   P   P       F + A     G+P  S+P +  +    
Sbjct: 541 GALTYTAKAANPFDLSYDDTAPNQTP------QFPTMAYVQGGGLPHVSSP-IGYSDSSS 600

Query: 635 LPADLFTLNYSSYP---------APVPGWHSVPPRPMGYVMPYNSAMPVPSFPQSSTSTN 678
             AD   L  S  P         +P    H  P   MG     N +   PSF Q  +   
Sbjct: 601 PAADSIGLMTSQSPFHATALSPNSPALASHLSPGALMGQQSQVNMS---PSFRQEYSG-- 625