Spg003446 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003446
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold4: 49197189 .. 49200191 (-)
RNA-Seq ExpressionSpg003446
SyntenySpg003446
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAACGAAACTGTCCATCGCTCCTATTCTTAGAAGCAGGTGTAATTCTGGGAATGATCTATTTTCGAACGCCAACAATGACACAACTTCCTTCATTCCCAATTACAACGAACCGGAAAAAAAGCTTTGCCCAACACACTCGCAGACAACCACGCAATTCTTCGAACAGCGGCTGGTTTCTATCCGGAGCTATTCCATTTTCCGTTCCGGCTCTGCAATGGCCGCTGTTCGAATTGTCTTCCTCTTCCTGATCTTCCTTCTTGGCGGTCTACCTCTCTCCTGTTCCCTCACCGAGAACGAGGCCCTCCTCAAGCTCAAGCAATCCTTCGCCCACTCCGAATCCCTCAATTCCTGGACCTCCGACTCCGTCCCCTGCTCCGCTCGATGGACCGGCATCATCTGTAACAGGGGCGTCATCACCGGCCTCCATCTCACGGGCCTCCAACTCTCCGGCAAGATCGACGTCGAAGCTCTCCTCGAGCTGCGCGGGCTTCGCACCATCAGCTTCGTCAACAACAGCTTCTCCGGGCCCATCCCGGAGTTGAACAGAATCGGCGTTCTCAAGTCCATGCTCCTGTCTGGGAACGACTTCTCCGGGGTCATACCAAACGACTTCTTTTCATCCCTGACGTCCCTCAAGAAGGTCTGGCTCTCCTTCAACAAGTTCTCCGGGAACATTCCACACTCTCTGGGACAACTCTCCCATCTGATCGAACTCCACCTCGAAAACAACCAATTCTCAGGGCCCATCCCCCACCTGAAGCAGGCCTCCATAACCTCCCTCGACTTCTCCAACAACAAGCTAGAGGGAGAGATCCCGGAGAGCCTATCCAGATTCGAAGCAAAGGCATTTGCCGGGAACGAGGCCCTGTGCGGGAAACCGCTCCCCAAATCATGCGACAACAACGCGAGGATTTCGGAGGAAAAGCCCCCTCCGTCTCCCCCCGGGGAGTCCCAAGGCAACGTCTCCAAGCTAGTAGTGGCGTCGTTGATAGCGGTGGTCGTGTTTCTGATGGTCTTCATATTTCTGAGCGCGTCCAAGCGGAGAGAGGACGAGTTCAGCGTGCTGGGGAGGGAGCACATGGAGGAGGTGGTGGAGGTGCACGTGCCGCATGATAAGGGGGGCAGGGAGCAGTCGAGCAGGAGAGGTGGGGACTCGAGGAGAGGGTCGCAACAGGGGAAGGGGGCGATGAGCGATCTGGTGGTAGTGAATGATGACAAGGGTACGTTCGGGTTGGCGGATTTGATGAAGGCCGCGGCGGAGGTGCTGGGGAATGGTGGCTTGGGCTCTGCCTACAAGGCGGTGATGGCCAACGGGCTGTCGGTGGTGGTGAAGAGAATGAGAGAAATGAACAAACTGGGGAAGGATGGGTTCGATGCAGAGATGAGGCGGCTGGGAAAGCTGCGACATCAGAACATACTCACGCCTTTGGCCTACCATTACAGGAGAGAGGAGAAGCTGCTGGTGTCGGAGTACATTCCAAAGGGAAGCCTCTTGTATGTGTTGCATGGTACGTAAATCTATCTAATCGCCAGCTAAACCAATTATAATAGAATTAGAATTAAAATTAAAATTAAAATTGATGGATGGTCGTATACATATATACATACACATGATATAATTAAACCAGGTGACCGTGGAGCCTGCCATGCGGAGCTTAACTGGGGCACCCGGTTAAGGATTGTGAAGGGGATCGGCAGGGGTATGGGGTTCCTCCATTCCGAATTCGCGACCTACGATGTTCCGCATGGCAATCTGAAATCCAGCAACGTTCTTCTAAGCGACAACTACGAGCCCCTCCTCAGCGACTACGCCTTTCATCCGCTAATCAATCCCAACAATGCCACGCAGGCGATGTTTGCGTACAGATCCCCTGAGTACACCCAATACCAGGAACTTTCCCCCAAGTCTGACGTTTACTGCCTTGGAATTATCATTCTCGAAATTATGACCAGCAAATTCCCCTCTCAATATCTTAGCAATGGCAAGGGCGGTACCGATGTGGTGCAGTGGGTCTCCTCCGCAGTTTCCGAGAAGAGGGAGGCCGAACTCATCGACCCTGAGATTGCAAACGACACCGGTGCACTCGATCGCATGGTCCAACTCCTCAGAATCGGCGCCGATTGCACTCTCAGCAATCCTCAGCAACGGCCAGAAATGAGGGAAGCCATTAGGAGAATAGAAGAGATATGACAAGATCAGAAAGAGGAAAATTCATTTTAAGCTGCAAATACTTTTCTTTGTATTTTTTCTAAAAAAAAAAAAAAAAAATTAAAAAGACTAAAATGGCAAATTAAAGGAAAAAACAAAAAAACTAATGTGGTAGACGGATAGTTTGATTGGAATTTGTGAGTAAGTAAAGTAAAGTAAAGTAAATTAAAGTAGGGAAGCGATGGAGCAGGAGGTAGGAGGAGAAGTTTGTGAGCGTCTCAATCCCCTGCCGGCCGCCGCAACACGCCACCAGATCATATCTCGGATCGGATCGATCATGCATGTTCCTCCGCCCGAGACTCCGCCTGTTTCTGTGTAAGATCATTTATGTATAATGCATCACTTCACCTCTGTATTTTTTTGCATGTAAATGAATTTAACACTGGGGGGTTGGGATTTGTTAATTAGTTTTTTTCTTTCTTTCTTTTTTTTTTATTCGGTTCTTCAACCTTAATAGAAAACAATCACTTGTGATTTTGCAGTTTCTCAGAATTTCATCAAACATTTTTTGTATTTTCCACTTTAACTCCATAATCATCGTTATCCTATTAAGCTAAACATCCTTTTGATCTATTTGGTCCTTGATTTTACAAGTGATATGTTTTGGCCACTAAAAGTTAGATCTCACCTGGTAGTTAACCAATTATCAATGACATTGCCAATAAAAATGGGAGGTTTAAAACACTTTTGGCTCATCAATTTTAATAATTTAGACCCGATAAACATTAAACACTACTTTGCCCGTAAGTTTTTTTATTTTTTTTATTTTTATCCTCTTCCCATGTTTTA

mRNA sequence

ATGTCAACGAAACTGTCCATCGCTCCTATTCTTAGAAGCAGGTGTAATTCTGGGAATGATCTATTTTCGAACGCCAACAATGACACAACTTCCTTCATTCCCAATTACAACGAACCGGAAAAAAAGCTTTGCCCAACACACTCGCAGACAACCACGCAATTCTTCGAACAGCGGCTGGTTTCTATCCGGAGCTATTCCATTTTCCGTTCCGGCTCTGCAATGGCCGCTGTTCGAATTGTCTTCCTCTTCCTGATCTTCCTTCTTGGCGGTCTACCTCTCTCCTGTTCCCTCACCGAGAACGAGGCCCTCCTCAAGCTCAAGCAATCCTTCGCCCACTCCGAATCCCTCAATTCCTGGACCTCCGACTCCGTCCCCTGCTCCGCTCGATGGACCGGCATCATCTGTAACAGGGGCGTCATCACCGGCCTCCATCTCACGGGCCTCCAACTCTCCGGCAAGATCGACGTCGAAGCTCTCCTCGAGCTGCGCGGGCTTCGCACCATCAGCTTCGTCAACAACAGCTTCTCCGGGCCCATCCCGGAGTTGAACAGAATCGGCGTTCTCAAGTCCATGCTCCTGTCTGGGAACGACTTCTCCGGGGTCATACCAAACGACTTCTTTTCATCCCTGACGTCCCTCAAGAAGGTCTGGCTCTCCTTCAACAAGTTCTCCGGGAACATTCCACACTCTCTGGGACAACTCTCCCATCTGATCGAACTCCACCTCGAAAACAACCAATTCTCAGGGCCCATCCCCCACCTGAAGCAGGCCTCCATAACCTCCCTCGACTTCTCCAACAACAAGCTAGAGGGAGAGATCCCGGAGAGCCTATCCAGATTCGAAGCAAAGGCATTTGCCGGGAACGAGGCCCTGTGCGGGAAACCGCTCCCCAAATCATGCGACAACAACGCGAGGATTTCGGAGGAAAAGCCCCCTCCGTCTCCCCCCGGGGAGTCCCAAGGCAACGTCTCCAAGCTAGTAGTGGCGTCGTTGATAGCGGTGGTCGTGTTTCTGATGGTCTTCATATTTCTGAGCGCGTCCAAGCGGAGAGAGGACGAGTTCAGCGTGCTGGGGAGGGAGCACATGGAGGAGGTGGTGGAGGTGCACGTGCCGCATGATAAGGGGGGCAGGGAGCAGTCGAGCAGGAGAGGTGGGGACTCGAGGAGAGGGTCGCAACAGGGGAAGGGGGCGATGAGCGATCTGGTGGTAGTGAATGATGACAAGGGTACGTTCGGGTTGGCGGATTTGATGAAGGCCGCGGCGGAGGTGCTGGGGAATGGTGGCTTGGGCTCTGCCTACAAGGCGGTGATGGCCAACGGGCTGTCGGTGGTGGTGAAGAGAATGAGAGAAATGAACAAACTGGGGAAGGATGGGTTCGATGCAGAGATGAGGCGGCTGGGAAAGCTGCGACATCAGAACATACTCACGCCTTTGGCCTACCATTACAGGAGAGAGGAGAAGCTGCTGGTGTCGGAGTACATTCCAAAGGGAAGCCTCTTGTATGTGTTGCATGGTGACCGTGGAGCCTGCCATGCGGAGCTTAACTGGGGCACCCGGTTAAGGATTGTGAAGGGGATCGGCAGGGGTATGGGGTTCCTCCATTCCGAATTCGCGACCTACGATGTTCCGCATGGCAATCTGAAATCCAGCAACGTTCTTCTAAGCGACAACTACGAGCCCCTCCTCAGCGACTACGCCTTTCATCCGCTAATCAATCCCAACAATGCCACGCAGGCGATGTTTGCGTACAGATCCCCTGAGTACACCCAATACCAGGAACTTTCCCCCAAGTCTGACGTTTACTGCCTTGGAATTATCATTCTCGAAATTATGACCAGCAAATTCCCCTCTCAATATCTTAGCAATGGCAAGGGCGGTACCGATGTGGTGCAGTGGGTCTCCTCCGCAGTTTCCGAGAAGAGGGAGGCCGAACTCATCGACCCTGAGATTGCAAACGACACCGGTGCACTCGATCGCATGGTCCAACTCCTCAGAATCGGCGCCGATTGCACTCTCAGCAATCCTCAGCAACGGCCAGAAATGAGGGAAGCCATTAGGAGAATAGAAGAGATATGA

Coding sequence (CDS)

ATGTCAACGAAACTGTCCATCGCTCCTATTCTTAGAAGCAGGTGTAATTCTGGGAATGATCTATTTTCGAACGCCAACAATGACACAACTTCCTTCATTCCCAATTACAACGAACCGGAAAAAAAGCTTTGCCCAACACACTCGCAGACAACCACGCAATTCTTCGAACAGCGGCTGGTTTCTATCCGGAGCTATTCCATTTTCCGTTCCGGCTCTGCAATGGCCGCTGTTCGAATTGTCTTCCTCTTCCTGATCTTCCTTCTTGGCGGTCTACCTCTCTCCTGTTCCCTCACCGAGAACGAGGCCCTCCTCAAGCTCAAGCAATCCTTCGCCCACTCCGAATCCCTCAATTCCTGGACCTCCGACTCCGTCCCCTGCTCCGCTCGATGGACCGGCATCATCTGTAACAGGGGCGTCATCACCGGCCTCCATCTCACGGGCCTCCAACTCTCCGGCAAGATCGACGTCGAAGCTCTCCTCGAGCTGCGCGGGCTTCGCACCATCAGCTTCGTCAACAACAGCTTCTCCGGGCCCATCCCGGAGTTGAACAGAATCGGCGTTCTCAAGTCCATGCTCCTGTCTGGGAACGACTTCTCCGGGGTCATACCAAACGACTTCTTTTCATCCCTGACGTCCCTCAAGAAGGTCTGGCTCTCCTTCAACAAGTTCTCCGGGAACATTCCACACTCTCTGGGACAACTCTCCCATCTGATCGAACTCCACCTCGAAAACAACCAATTCTCAGGGCCCATCCCCCACCTGAAGCAGGCCTCCATAACCTCCCTCGACTTCTCCAACAACAAGCTAGAGGGAGAGATCCCGGAGAGCCTATCCAGATTCGAAGCAAAGGCATTTGCCGGGAACGAGGCCCTGTGCGGGAAACCGCTCCCCAAATCATGCGACAACAACGCGAGGATTTCGGAGGAAAAGCCCCCTCCGTCTCCCCCCGGGGAGTCCCAAGGCAACGTCTCCAAGCTAGTAGTGGCGTCGTTGATAGCGGTGGTCGTGTTTCTGATGGTCTTCATATTTCTGAGCGCGTCCAAGCGGAGAGAGGACGAGTTCAGCGTGCTGGGGAGGGAGCACATGGAGGAGGTGGTGGAGGTGCACGTGCCGCATGATAAGGGGGGCAGGGAGCAGTCGAGCAGGAGAGGTGGGGACTCGAGGAGAGGGTCGCAACAGGGGAAGGGGGCGATGAGCGATCTGGTGGTAGTGAATGATGACAAGGGTACGTTCGGGTTGGCGGATTTGATGAAGGCCGCGGCGGAGGTGCTGGGGAATGGTGGCTTGGGCTCTGCCTACAAGGCGGTGATGGCCAACGGGCTGTCGGTGGTGGTGAAGAGAATGAGAGAAATGAACAAACTGGGGAAGGATGGGTTCGATGCAGAGATGAGGCGGCTGGGAAAGCTGCGACATCAGAACATACTCACGCCTTTGGCCTACCATTACAGGAGAGAGGAGAAGCTGCTGGTGTCGGAGTACATTCCAAAGGGAAGCCTCTTGTATGTGTTGCATGGTGACCGTGGAGCCTGCCATGCGGAGCTTAACTGGGGCACCCGGTTAAGGATTGTGAAGGGGATCGGCAGGGGTATGGGGTTCCTCCATTCCGAATTCGCGACCTACGATGTTCCGCATGGCAATCTGAAATCCAGCAACGTTCTTCTAAGCGACAACTACGAGCCCCTCCTCAGCGACTACGCCTTTCATCCGCTAATCAATCCCAACAATGCCACGCAGGCGATGTTTGCGTACAGATCCCCTGAGTACACCCAATACCAGGAACTTTCCCCCAAGTCTGACGTTTACTGCCTTGGAATTATCATTCTCGAAATTATGACCAGCAAATTCCCCTCTCAATATCTTAGCAATGGCAAGGGCGGTACCGATGTGGTGCAGTGGGTCTCCTCCGCAGTTTCCGAGAAGAGGGAGGCCGAACTCATCGACCCTGAGATTGCAAACGACACCGGTGCACTCGATCGCATGGTCCAACTCCTCAGAATCGGCGCCGATTGCACTCTCAGCAATCCTCAGCAACGGCCAGAAATGAGGGAAGCCATTAGGAGAATAGAAGAGATATGA

Protein sequence

MSTKLSIAPILRSRCNSGNDLFSNANNDTTSFIPNYNEPEKKLCPTHSQTTTQFFEQRLVSIRSYSIFRSGSAMAAVRIVFLFLIFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTGIICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLKQASITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNNARISEEKPPPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVPHDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRPEMREAIRRIEEI
Homology
BLAST of Spg003446 vs. NCBI nr
Match: XP_008460083.1 (PREDICTED: pollen receptor-like kinase 3 [Cucumis melo])

HSP 1 Score: 1058.1 bits (2735), Expect = 3.2e-305
Identity = 542/629 (86.17%), Postives = 574/629 (91.26%), Query Frame = 0

Query: 65  YSIFRSGSAMAAVRIVFLFLIFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSV 124
           +SIF  GSAM AVRIVFL ++   GGLPL C L+ENEALLKLK+SF HSESLNSW  DSV
Sbjct: 44  FSIFPFGSAMGAVRIVFLIILVGGGGLPLCCPLSENEALLKLKESFTHSESLNSWNPDSV 103

Query: 125 PCSARWTGIICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNR 184
           PCSA+WTGIICNRGVITGLHL+ LQLSGKIDVEALL+LRGLRTISFVNN FSGPIPE N+
Sbjct: 104 PCSAQWTGIICNRGVITGLHLSDLQLSGKIDVEALLQLRGLRTISFVNNKFSGPIPEFNK 163

Query: 185 IGVLKSMLLSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLEN 244
           IGVLKS+LL+GN FSG IP+DFFSSLTSLKKVWLS N FSGNIPHSL QLSHLIELHLE+
Sbjct: 164 IGVLKSLLLTGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLES 223

Query: 245 NQFSGPIPHLKQAS-ITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNN 304
           NQFSGPIPHLK AS ITSLD SNNKLEG+IP+ LS+F+AKAFAGNE LCG PLPKSC   
Sbjct: 224 NQFSGPIPHLKHASIITSLDLSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSC--G 283

Query: 305 ARISE-EKPPPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHM 364
           ARI E +KP  SPPGESQGNVSKLVVASLIAV VFLMVFIFLSASKRREDEFSVLGRE M
Sbjct: 284 ARIPEDQKPSSSPPGESQGNVSKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGRERM 343

Query: 365 EEVVEVHVPHDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAE 424
           EEVVEVHVP     ++ S + GGDS+RGSQQGK  MSDLVVVN+DKG FGLADLMKAAAE
Sbjct: 344 EEVVEVHVPSSGHDKQSSRKGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAE 403

Query: 425 VLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHY 484
           VLGNGGLGSAYKAVM+NGLSVVVKRMREMNKLGKDGFDAEMRRLG+LRH NILTPLAYHY
Sbjct: 404 VLGNGGLGSAYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHY 463

Query: 485 RREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDV 544
           RREEKLLVSEYIPKGSLLYVLHGDRGACHA+LNW TRLRIV+GI RG+GFLHSEFATYD+
Sbjct: 464 RREEKLLVSEYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDL 523

Query: 545 PHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYC 604
           PHGNLKSSNVLL DNYEPLLSDYAFHPLINPNNATQAMFAYRSPEY QYQE+SPKSDVYC
Sbjct: 524 PHGNLKSSNVLLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYC 583

Query: 605 LGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQ 664
           LGIIILEIMTSKFPSQYL+NGKGGTDVVQWVSSAVSEKREAELIDPEIANDT ALDRMV 
Sbjct: 584 LGIIILEIMTSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVN 643

Query: 665 LLRIGADCTLSNPQQRPEMREAIRRIEEI 692
           LL IGADCT +NPQQRPEMREAIRRIEEI
Sbjct: 644 LLTIGADCTHNNPQQRPEMREAIRRIEEI 670

BLAST of Spg003446 vs. NCBI nr
Match: XP_004141181.2 (pollen receptor-like kinase 3 [Cucumis sativus])

HSP 1 Score: 1057.7 bits (2734), Expect = 4.2e-305
Identity = 541/629 (86.01%), Postives = 575/629 (91.41%), Query Frame = 0

Query: 65  YSIFRSGSAMAAVRIVFLFLIFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSV 124
           +SIF  GSAM AVRIVFL ++   GGLP  C L+ENEALLKLK+SF HSESLNSW  DSV
Sbjct: 19  FSIFPFGSAMGAVRIVFLIILVGGGGLPSCCPLSENEALLKLKESFTHSESLNSWNPDSV 78

Query: 125 PCSARWTGIICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNR 184
           PCSARW GIICNRGVITGLHL+GLQLSGKIDVEALL+LRGLRTISFV+N FSGPIPE N+
Sbjct: 79  PCSARWIGIICNRGVITGLHLSGLQLSGKIDVEALLQLRGLRTISFVDNQFSGPIPEFNK 138

Query: 185 IGVLKSMLLSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLEN 244
           IGVLKS+LL+GN FSG IP+DFFSSLTSLKKVWLS N FSGNIPHSL QLSHLIELHLE+
Sbjct: 139 IGVLKSLLLTGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLES 198

Query: 245 NQFSGPIPHLKQAS-ITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNN 304
           NQFSGPIPHLK AS ITSL+ SNNKLEG+IP+ LS+F+AKAFAGNE LCG PLPKSC   
Sbjct: 199 NQFSGPIPHLKHASIITSLNVSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSC--G 258

Query: 305 ARISE-EKPPPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHM 364
           A+ISE +KPP SPPGESQGN+SKLVVASLIAV VFLMVFIFLSASKRREDEFSVLGRE M
Sbjct: 259 AQISEDQKPPSSPPGESQGNISKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGREQM 318

Query: 365 EEVVEVHVPHDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAE 424
           EEVVEVHVP     ++ S R GGDS+RGSQQGK  MSDLVVVN+DKG FGLADLMKAAAE
Sbjct: 319 EEVVEVHVPSSGHDKQSSRRGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAE 378

Query: 425 VLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHY 484
           VLGNGGLGSAYKAVM+NGLSVVVKRMREMNKLGKDGFDAEMRRLG+LRH NILTPLAYHY
Sbjct: 379 VLGNGGLGSAYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHY 438

Query: 485 RREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDV 544
           RREEKLLVSEYIPKGSLLYVLHGDRGACHA+LNW TRLRIV+GI RG+GFLHSEFATYD+
Sbjct: 439 RREEKLLVSEYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDL 498

Query: 545 PHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYC 604
           PHGNLKSSNVLL DNYEPLLSDYAFHPLINPNNATQAMFAYRSPEY QYQE+SPKSDVYC
Sbjct: 499 PHGNLKSSNVLLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYC 558

Query: 605 LGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQ 664
           LGIIILEIMTSKFPSQYL+NGKGGTDVVQWVSSAVSEKREAELIDPEIANDT ALDRMV 
Sbjct: 559 LGIIILEIMTSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVH 618

Query: 665 LLRIGADCTLSNPQQRPEMREAIRRIEEI 692
           LL IGADCT +NPQQRPEMREAIRRIEEI
Sbjct: 619 LLTIGADCTHNNPQQRPEMREAIRRIEEI 645

BLAST of Spg003446 vs. NCBI nr
Match: KAA0061448.1 (pollen receptor-like kinase 3 [Cucumis melo var. makuwa] >TYK10833.1 pollen receptor-like kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.9e-303
Identity = 537/620 (86.61%), Postives = 568/620 (91.61%), Query Frame = 0

Query: 74  MAAVRIVFLFLIFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTGI 133
           M AVRIVFL ++   GGLPL C L+ENEALLKLK+SF HSESLNSW  DSVPCSA+WTGI
Sbjct: 1   MGAVRIVFLIILVGGGGLPLCCPLSENEALLKLKESFTHSESLNSWNPDSVPCSAQWTGI 60

Query: 134 ICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLL 193
           ICNRGVITGLHL+ LQLSGKIDVEALL+LRGLRTISFVNN FSGPIPE N+IGVLKS+LL
Sbjct: 61  ICNRGVITGLHLSDLQLSGKIDVEALLQLRGLRTISFVNNKFSGPIPEFNKIGVLKSLLL 120

Query: 194 SGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPH 253
           +GN FSG IP+DFFSSLTSLKKVWLS N FSGNIPHSL QLSHLIELHLE+NQFSGPIPH
Sbjct: 121 TGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLESNQFSGPIPH 180

Query: 254 LKQAS-ITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNNARISE-EKP 313
           LK AS ITSLD SNNKLEG+IP+ LS+F+AKAFAGNE LCG PLPKSC   ARI E +KP
Sbjct: 181 LKHASIITSLDLSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSC--GARIPEDQKP 240

Query: 314 PPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVP 373
           P SPPGESQGNVSKLVVASLIAV VFLMVFIFLSASKRREDEFSVLGRE MEEVVEVHVP
Sbjct: 241 PSSPPGESQGNVSKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGREQMEEVVEVHVP 300

Query: 374 HDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGS 433
                ++ S + GGDS+RGSQQGK  MSDLVVVN+DKG FGLADLMKAAAEVLGNGGLGS
Sbjct: 301 SSGHDKQSSRKGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAEVLGNGGLGS 360

Query: 434 AYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVS 493
           AYKAVM+NGLSVVVKRMREMNKLGKDGFDAEMRRLG+LRH NILTPLAYHYRREEKLLVS
Sbjct: 361 AYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHYRREEKLLVS 420

Query: 494 EYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSN 553
           EYIPKGSLLYVLHGDRGACHA+LNW TRLRIV+GI RG+GFLHSEFATYD+PHGNLKSSN
Sbjct: 421 EYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDLPHGNLKSSN 480

Query: 554 VLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIM 613
           VLL DNYEPLLSDYAFHPLINPNNATQAMFAYRSPEY QYQE+SPKSDVYCLGIIILEIM
Sbjct: 481 VLLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYCLGIIILEIM 540

Query: 614 TSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCT 673
           TSKFPSQYL+NGKGGTDVVQWVSSAVSEKREAELIDPEIANDT ALDRMV LL IGADCT
Sbjct: 541 TSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVNLLTIGADCT 600

Query: 674 LSNPQQRPEMREAIRRIEEI 692
            +NPQQRPEMREAIRRIEEI
Sbjct: 601 HNNPQQRPEMREAIRRIEEI 618

BLAST of Spg003446 vs. NCBI nr
Match: XP_038889222.1 (pollen receptor-like kinase 3 [Benincasa hispida])

HSP 1 Score: 1046.2 bits (2704), Expect = 1.3e-301
Identity = 538/621 (86.63%), Postives = 571/621 (91.95%), Query Frame = 0

Query: 74  MAAVRIVFLFLIFLLG-GLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTG 133
           MAAV IV  FLI L+G GLP+ C L+ENEALLKLKQSF HSESLNSW  DSVPCSARWTG
Sbjct: 1   MAAVGIV--FLIVLVGVGLPVCCPLSENEALLKLKQSFTHSESLNSWNPDSVPCSARWTG 60

Query: 134 IICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSML 193
           IICNRGVITGLHL+GLQLSGKIDVEALL+LR LRTISFVNN FSGPIPE N+IGVLK++L
Sbjct: 61  IICNRGVITGLHLSGLQLSGKIDVEALLQLRSLRTISFVNNKFSGPIPEFNKIGVLKALL 120

Query: 194 LSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIP 253
           L+GN FSG IPNDFFSSLTSLKKVWLS N FSGNIPHSL QLSHLIELHLE+NQFSGPIP
Sbjct: 121 LTGNHFSGAIPNDFFSSLTSLKKVWLSSNNFSGNIPHSLSQLSHLIELHLESNQFSGPIP 180

Query: 254 HLKQAS-ITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSC-DNNARISEEK 313
            LK AS ITSLD SNNKLEG+IP++LS+F+AKAFAGNE LCG PLPKSC +N ARISEEK
Sbjct: 181 QLKYASIITSLDLSNNKLEGQIPDTLSKFDAKAFAGNEGLCGNPLPKSCGENAARISEEK 240

Query: 314 PPPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHV 373
            PPSPPGESQGN+SKLVVASLIAV VFLMVF+FLSASKRR+DEFSVLGRE MEEVVEVHV
Sbjct: 241 -PPSPPGESQGNISKLVVASLIAVAVFLMVFLFLSASKRRDDEFSVLGREQMEEVVEVHV 300

Query: 374 PHDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLG 433
           P     ++ S R GGDS+RGSQQGK  MSDLVVVN+DKGTFGLADLMKAAAEVLGNGGLG
Sbjct: 301 PSSGHDKQSSRRGGGDSKRGSQQGKAGMSDLVVVNEDKGTFGLADLMKAAAEVLGNGGLG 360

Query: 434 SAYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLV 493
           SAYKAVM+NGLSVVVKRMREMNKLGKDGFDAEMRRLG+L H+NILTPLAYHYRREEKLLV
Sbjct: 361 SAYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLSHRNILTPLAYHYRREEKLLV 420

Query: 494 SEYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSS 553
           SEYIPKGSLLYVLHGDRGACHA+LNW TRLRIV+GI RG+GFLHSEFATYD+PHGNLKSS
Sbjct: 421 SEYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDLPHGNLKSS 480

Query: 554 NVLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEI 613
           NVLLSDNYEPLLSDYAFHPLINPNNA QAMFAYRSPEY Q QE+SPKSDVYCLGIIILEI
Sbjct: 481 NVLLSDNYEPLLSDYAFHPLINPNNAIQAMFAYRSPEYAQSQEVSPKSDVYCLGIIILEI 540

Query: 614 MTSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADC 673
           MTSKFPSQYL+NGKGGTDVVQWVSSAVSEKREAELIDPEIAND  ALDRMV LL IGADC
Sbjct: 541 MTSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDADALDRMVNLLTIGADC 600

Query: 674 TLSNPQQRPEMREAIRRIEEI 692
           T +NPQQRPEMREAIRRIEEI
Sbjct: 601 THNNPQQRPEMREAIRRIEEI 618

BLAST of Spg003446 vs. NCBI nr
Match: XP_023537808.1 (pollen receptor-like kinase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1036.6 bits (2679), Expect = 9.9e-299
Identity = 524/620 (84.52%), Postives = 574/620 (92.58%), Query Frame = 0

Query: 74  MAAVRIVFLFL-IFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTG 133
           MAAVR VFLFL IFL+GG PLSCSL++NEALLKL+QSF H+ESLNSW  DS PC+ARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWNQDSQPCAARWTG 60

Query: 134 IICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSML 193
           IIC+RGVITGLHLTGL+LSG+ID+EALLELR LRT+S VNNSF+GPIPE NRIGVLKS+L
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLELRSLRTVSLVNNSFAGPIPEFNRIGVLKSLL 120

Query: 194 LSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIP 253
           L+GNDFSGVIP DFFSSLTSLKKVWLS N FSG+IPHSL +LS+LIELHLENNQFSG IP
Sbjct: 121 LTGNDFSGVIPTDFFSSLTSLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGKIP 180

Query: 254 HLKQASITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCD-NNARISEEKP 313
           HL QA++TSLD SNNKLEGEIPE+L++F+A AFAGNE LCGKPLPKSCD NNA+ISEE  
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEEN- 240

Query: 314 PPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVP 373
             SPPGESQG+VSKLVVASLIAV VFL+V +FLSASKRRE++FS LGRE M E+VEVHVP
Sbjct: 241 -ASPPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVP 300

Query: 374 HDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGS 433
              G REQ SR+GGDSRRGSQQGKG MSDLVVVN++KGTFG+ADLMKAAAEVLGNGGLGS
Sbjct: 301 -SSGHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGS 360

Query: 434 AYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVS 493
           AYKAVMANGLSVVVKR+REMNKLGKDGFDAEMRR+G+L+H NILTPLAYHYRREEKLLVS
Sbjct: 361 AYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVS 420

Query: 494 EYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSN 553
           EYIPKGSLLYVLHGDRGACHAELNWGTRL+IVKGI RG+GFLHSEFATYD+PHGNLKSSN
Sbjct: 421 EYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSN 480

Query: 554 VLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIM 613
           +LLSD YEPLLSDYAFHPLINPNNAT+AMF Y+SPEY Q QE+SPKSDVYCLGIIILEI+
Sbjct: 481 ILLSDTYEPLLSDYAFHPLINPNNATEAMFGYKSPEYAQRQEVSPKSDVYCLGIIILEIL 540

Query: 614 TSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCT 673
           T KFPSQYLSNGKGGTDVVQWVSSAV+EKREAELIDPEIAND  A+ RMV+LLRIGADCT
Sbjct: 541 TGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCT 600

Query: 674 LSNPQQRPEMREAIRRIEEI 692
            SNPQQRPEM+EAIRRIEEI
Sbjct: 601 QSNPQQRPEMKEAIRRIEEI 617

BLAST of Spg003446 vs. ExPASy Swiss-Prot
Match: Q9M1L7 (Pollen receptor-like kinase 3 OS=Arabidopsis thaliana OX=3702 GN=PRK3 PE=1 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.1e-186
Identity = 344/640 (53.75%), Postives = 458/640 (71.56%), Query Frame = 0

Query: 79  IVFL-FLIFLLGGLPLSCSLTENEALLKLKQSFAHSE-SLNSWTSDSVPCSARWTGIICN 138
           ++FL FL+      P   +++E+E L++ K+S   ++  LNSW + + PC+ +W GI C 
Sbjct: 4   VLFLCFLLICFSFTPSLQNVSESEPLVRFKRSVNITKGDLNSWRTGTDPCNGKWFGIYCQ 63

Query: 139 RG-VITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSG 198
           +G  ++G+H+T L LSG I++E L +L  LRTI   NN  SGP+P   ++  LKS+LLS 
Sbjct: 64  KGQTVSGIHVTRLGLSGTINIEDLKDLPNLRTIRLDNNLLSGPLPPFFKLPGLKSLLLSN 123

Query: 199 NDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLK 258
           N FSG I +DFF     LK+V+L  N+ SG IP SL QL+ L ELH++ NQF+G IP L 
Sbjct: 124 NSFSGEIADDFFKETPQLKRVFLDNNRLSGKIPASLMQLAGLEELHMQGNQFTGEIPPLT 183

Query: 259 QAS--ITSLDFSNNKLEGEIPESLS---RFEAKAFAGNEALCGKPLPKSCDNNARISEEK 318
             +  + SLD SNN LEGEIP ++S     E K F GN+ LCG PL   CD       EK
Sbjct: 184 DGNKVLKSLDLSNNDLEGEIPITISDRKNLEMK-FEGNQRLCGSPLNIECD-------EK 243

Query: 319 PPPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSA-----SKRREDEFSVLGREHM--E 378
           P  +  G  + N +K    ++  V++FL++F+F+ A      K+R+ EF +LG++H+  +
Sbjct: 244 PSSTGSGNEKNNTAK----AIFMVILFLLIFLFVVAIITRWKKKRQPEFRMLGKDHLSDQ 303

Query: 379 EVVEVHVPHD-----KGGREQSSRRGGDSRRGSQQGKGA-------MSDLVVVNDDKGTF 438
           E VEV VP          +++S+  G   +  S  GKGA       M D+++VN +KG+F
Sbjct: 304 ESVEVRVPDSIKKPIDSSKKRSNAEGSSKKGSSHNGKGAGGGPGSGMGDIIMVNSEKGSF 363

Query: 439 GLADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRH 498
           GL DLMKAAAEVLGNG LGSAYKAVMANGLSVVVKR+R+MNKL ++ FD EM+R GKLRH
Sbjct: 364 GLPDLMKAAAEVLGNGSLGSAYKAVMANGLSVVVKRIRDMNKLAREAFDTEMQRFGKLRH 423

Query: 499 QNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMG 558
            N+LTPLAYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL W TRL+I++G+ RGM 
Sbjct: 424 PNVLTPLAYHYRREEKLVVSEYMPKSSLLYVLHGDRGVYHSELTWATRLKIIQGVARGMD 483

Query: 559 FLHSEFATYDVPHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQY 618
           FLH EFA+YD+PHGNLKSSNVLLS+ YEPL+SDYAF PL+ PNNA+QA+FA++SPE+ Q 
Sbjct: 484 FLHEEFASYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPNNASQALFAFKSPEFVQN 543

Query: 619 QELSPKSDVYCLGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIA 678
           Q++SPKSDVYCLGII+LE+MT KFPSQYL+ GKGGTD+V+WV S++++ +E ELIDPEIA
Sbjct: 544 QQVSPKSDVYCLGIIVLEVMTGKFPSQYLNTGKGGTDIVEWVQSSIAQHKEEELIDPEIA 603

Query: 679 NDTGALDRMVQLLRIGADCTLSNPQQRPEMREAIRRIEEI 692
           ++T ++ +MV+LLRIGA C  SNP +R  M+E +RRIE +
Sbjct: 604 SNTDSIKQMVELLRIGAACIASNPNERQNMKEIVRRIERV 631

BLAST of Spg003446 vs. ExPASy Swiss-Prot
Match: Q3E991 (Pollen receptor-like kinase 6 OS=Arabidopsis thaliana OX=3702 GN=PRK6 PE=1 SV=4)

HSP 1 Score: 616.3 bits (1588), Expect = 4.2e-175
Identity = 332/655 (50.69%), Postives = 449/655 (68.55%), Query Frame = 0

Query: 75  AAVRIVFLFLIFLLGGLPLSCSL---TENEALLKLKQSFAHSE-SLNSWTSDSVPCSARW 134
           AAV     FL+ LL    +S SL   +E+E L++ K S   ++  LNSW   + PCS +W
Sbjct: 3   AAVLNPGFFLLILLLSFSISPSLQYVSESEPLVRFKNSVKITKGDLNSWREGTDPCSGKW 62

Query: 135 TGIICNRGV-ITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLK 194
            GI C +G+ ++G+H+T L LSG I V+ L +L  L+TI   NN  SGP+P   ++  LK
Sbjct: 63  FGIYCQKGLTVSGIHVTRLGLSGTITVDDLKDLPNLKTIRLDNNLLSGPLPHFFKLRGLK 122

Query: 195 SMLLSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSG 254
           S++LS N FSG I +DFF  ++ LK+++L  NKF G+IP S+ QL  L ELH+++N  +G
Sbjct: 123 SLMLSNNSFSGEIRDDFFKDMSKLKRLFLDHNKFEGSIPSSITQLPQLEELHMQSNNLTG 182

Query: 255 PIP--HLKQASITSLDFSNNKLEGEIPESLS--RFEAKAFAGNEALCGKPLPKSCDN-NA 314
            IP       ++  LD S N L+G +P+S++  +  A     NE LCG  +   C+N   
Sbjct: 183 EIPPEFGSMKNLKVLDLSTNSLDGIVPQSIADKKNLAVNLTENEYLCGPVVDVGCENIEL 242

Query: 315 RISEEKPPPSPPGES------QGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLG 374
              +E  PPS P  S      +  ++ ++V+  + ++ F++V +    +K++  +F +L 
Sbjct: 243 NDPQEGQPPSKPSSSVPETSNKAAINAIMVSISLLLLFFIIVGVIKRRNKKKNPDFRMLA 302

Query: 375 REHMEEVVEVHVPHD-----KGGREQSSRRGGDSRRGSQQ------GK-----------G 434
                +VVEV +        K   + S +RGG S  GS +      GK           G
Sbjct: 303 NNRENDVVEVRISESSSTTAKRSTDSSRKRGGHSDDGSTKKGVSNIGKGGNGGGGGALGG 362

Query: 435 AMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGK 494
            M D+++VN DKG+FGL DLMKAAAEVLGNG LGSAYKAVM  GLSVVVKR+R+MN+L +
Sbjct: 363 GMGDIIMVNTDKGSFGLPDLMKAAAEVLGNGSLGSAYKAVMTTGLSVVVKRIRDMNQLAR 422

Query: 495 DGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNW 554
           + FD EMRR GKLRH NILTPLAYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL W
Sbjct: 423 EPFDVEMRRFGKLRHPNILTPLAYHYRREEKLVVSEYMPKSSLLYVLHGDRGIYHSELTW 482

Query: 555 GTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNA 614
            TRL+I++G+  GM FLH EFA+YD+PHGNLKSSNVLLS+ YEPL+SDYAF PL+ P+NA
Sbjct: 483 ATRLKIIQGVAHGMKFLHEEFASYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPSNA 542

Query: 615 TQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSA 674
           +QA+FA+++PE+ Q Q++S KSDVYCLGIIILEI+T KFPSQYL+NGKGGTD+VQWV S+
Sbjct: 543 SQALFAFKTPEFAQTQQVSHKSDVYCLGIIILEILTGKFPSQYLNNGKGGTDIVQWVQSS 602

Query: 675 VSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRPEMREAIRRIEEI 692
           V+E++E ELIDPEI N+T ++ +MV+LLR+GA C  SNP +R +MREA+RRIE++
Sbjct: 603 VAEQKEEELIDPEIVNNTESMRQMVELLRVGAACIASNPDERLDMREAVRRIEQV 657

BLAST of Spg003446 vs. ExPASy Swiss-Prot
Match: Q9LJY0 (Pollen receptor-like kinase 4 OS=Arabidopsis thaliana OX=3702 GN=PRK4 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.0e-125
Identity = 245/612 (40.03%), Postives = 370/612 (60.46%), Query Frame = 0

Query: 91  LPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPC---SARWTGIICNRGVITGLHLTG 150
           LPL  S  + + LL+ K +  ++  ++SW     PC   S  W G++C  G + GL L G
Sbjct: 46  LPLPAS--DADCLLRFKDTLVNASFISSWDPSISPCKRNSENWFGVLCVTGNVWGLQLEG 105

Query: 151 LQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSGNDFSGVIPNDFF 210
           + L+GK+D+E L  ++ LRT+SF+NN F+G +P +   G LKS+ LS N F+G IP D F
Sbjct: 106 MGLTGKLDLEPLAAIKNLRTLSFMNNKFNGSMPSVKNFGALKSLYLSNNRFTGEIPADAF 165

Query: 211 SSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLKQASITSLDFSNN 270
             +  LKK+ L+ N F G+IP SL  L  L+EL L  NQF G IP+ KQ  +    F NN
Sbjct: 166 DGMHHLKKLLLANNAFRGSIPSSLAYLPMLLELRLNGNQFHGEIPYFKQKDLKLASFENN 225

Query: 271 KLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNNARISEEKPPPSPPGESQGNVS--- 330
            LEG IPESLS  +  +F+GN+ LCG PL   C +++  S +   PS P E   N S   
Sbjct: 226 DLEGPIPESLSNMDPVSFSGNKNLCGPPL-SPCSSDSGSSPDL--PSSPTEKNKNQSFFI 285

Query: 331 ---KLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEV-VEVHVPHDKGGREQS 390
               L+V  +I +++ L+V I  +  ++    +   G++  E+   +     DK     +
Sbjct: 286 IAIVLIVIGIILMIISLVVCILHTRRRKSLSAYPSAGQDRTEKYNYDQSTDKDKAADSVT 345

Query: 391 SRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANG 450
           S     SRRG+   +   + L+ + DD   F L DL++A+AEVLG+G  GS+YK  + +G
Sbjct: 346 SY---TSRRGAVPDQ---NKLLFLQDDIQRFDLQDLLRASAEVLGSGSFGSSYKTGINSG 405

Query: 451 LSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLL 510
             +VVKR + MN +G+D F   MRRLG+L+H N+L  +AY+YRREEKLL++E++P  SL 
Sbjct: 406 QMLVVKRYKHMNNVGRDEFHEHMRRLGRLKHPNLLPIVAYYYRREEKLLIAEFMPNRSLA 465

Query: 511 YVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEP 570
             LH +       L+W TRL+I++G+ +G+G+L +E  T  +PHG+LKSSNV+L +++EP
Sbjct: 466 SHLHANHSVDQPGLDWPTRLKIIQGVAKGLGYLFNELTTLTIPHGHLKSSNVVLDESFEP 525

Query: 571 LLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIMTSKFPSQYL 630
           LL+DYA  P++N   +   M +Y+SPEY+    L+ K+DV+CLG++ILE++T +FP  YL
Sbjct: 526 LLTDYALRPVMNSEQSHNLMISYKSPEYSLKGHLTKKTDVWCLGVLILELLTGRFPENYL 585

Query: 631 SNG-KGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRP 690
           S G      +V WVS+ V EK+  ++ D E+         M+ LL+IG  C   + ++R 
Sbjct: 586 SQGYDANMSLVTWVSNMVKEKKTGDVFDKEMTGKKNCKAEMLNLLKIGLSCCEEDEERRM 645

Query: 691 EMREAIRRIEEI 692
           EMR+A+ +IE +
Sbjct: 646 EMRDAVEKIERL 646

BLAST of Spg003446 vs. ExPASy Swiss-Prot
Match: C0LGU0 (Pollen receptor-like kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PRK1 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.3e-120
Identity = 245/609 (40.23%), Postives = 373/609 (61.25%), Query Frame = 0

Query: 97  LTENEALLKLKQSF--AHSESLNSWTSDSVPCSARWTGIICNRGVITGLHLTGLQLSGKI 156
           L+++EA+LK K+S       +L SW + S PC+  W+G++CN G +  L +  L+LSG I
Sbjct: 32  LSDSEAILKFKESLVVGQENALASWNAKSPPCT--WSGVLCNGGSVWRLQMENLELSGSI 91

Query: 157 DVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSGNDFSGVIPNDFFSSLTSLK 216
           D+EAL  L  LRT+SF+NN F GP P+  ++  LKS+ LS N F G IP D F  +  LK
Sbjct: 92  DIEALSGLTSLRTLSFMNNKFEGPFPDFKKLAALKSLYLSNNQFGGDIPGDAFEGMGWLK 151

Query: 217 KVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLKQASITSLDFSNNKLEGEIP 276
           KV L+ NKF+G IP S+ +L  L+EL L+ NQF+G IP  +   +  L+ SNN L G IP
Sbjct: 152 KVHLAQNKFTGQIPSSVAKLPKLLELRLDGNQFTGEIPEFEH-QLHLLNLSNNALTGPIP 211

Query: 277 ESLSRFEAKAFAGNEALCGKPLPKSCDNNARISEEKPPPS---PPGESQGN-VSKLVVAS 336
           ESLS  + K F GN+ L GKPL   CD+      E PP S   P   S+G  V   +VA+
Sbjct: 212 ESLSMTDPKVFEGNKGLYGKPLETECDSP---YIEHPPQSEARPKSSSRGPLVITAIVAA 271

Query: 337 LIAVVVFLMVFIFLSASKRREDEFSV-LGREHMEEVVEVHVPHDKGGREQSSRRGGDSRR 396
           L  +++  ++F+   + K ++   +V  G   +++   +     +  + +  R+  D R+
Sbjct: 272 LTILIILGVIFLLNRSYKNKKPRLAVETGPSSLQKKTGIR----EADQSRRDRKKADHRK 331

Query: 397 GSQQGK--GAM-----SDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANGLS 456
           GS   K  GA      + L  + +D+  F L DL+KA+AE+LG+G  G++YKAV+++G  
Sbjct: 332 GSGTTKRMGAAAGVENTKLSFLREDREKFDLQDLLKASAEILGSGCFGASYKAVLSSGQM 391

Query: 457 VVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLLYV 516
           +VVKR ++MN  G+D F   M+RLG+L H N+L+ +AY+YR+EEKLLV ++  +GSL   
Sbjct: 392 MVVKRFKQMNNAGRDEFQEHMKRLGRLMHHNLLSIVAYYYRKEEKLLVCDFAERGSLAIN 451

Query: 517 LHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEPLL 576
           LH ++      L+W TRL+IVKG+ +G+ +LH +  +   PHG+LKSSNVLL+  +EPLL
Sbjct: 452 LHSNQSLGKPSLDWPTRLKIVKGVAKGLFYLHQDLPSLMAPHGHLKSSNVLLTKTFEPLL 511

Query: 577 SDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIMTSKFPSQYLSN 636
           +DY   PLIN   A   M AYRSPEY Q++ ++ K+DV+ LGI+ILEI+T KFP+ +  +
Sbjct: 512 TDYGLIPLINQEKAQMHMAAYRSPEYLQHRRITKKTDVWGLGILILEILTGKFPANFSQS 571

Query: 637 GKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRPEMR 692
            +   D+  WV+S         L D  +   +    ++++LL IG +C   + ++R ++ 
Sbjct: 572 SE--EDLASWVNSGFHGVWAPSLFDKGMGKTSHCEGQILKLLTIGLNCCEPDVEKRLDIG 628

BLAST of Spg003446 vs. ExPASy Swiss-Prot
Match: Q9LPT1 (Pollen receptor-like kinase 5 OS=Arabidopsis thaliana OX=3702 GN=PRK5 PE=1 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.7e-120
Identity = 234/607 (38.55%), Postives = 359/607 (59.14%), Query Frame = 0

Query: 98  TENEALLKLKQSFAHSESLNSWTSDSVPC---SARWTGIICNRGVITGLHLTGLQLSGKI 157
           ++ + LL+ K + A+     SW   S PC   +A W G++C+   + GL L G+ L+GK+
Sbjct: 46  SDADCLLRFKDTLANGSEFRSWDPLSSPCQGNTANWFGVLCS-NYVWGLQLEGMGLTGKL 105

Query: 158 DVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSGNDFSGVIPNDFFSSLTSLK 217
           +++ L+ ++ LRTISF+NN+F+GP+P++ R   LKS+ LS N FSG IP D F  +  LK
Sbjct: 106 NLDPLVPMKNLRTISFMNNNFNGPMPQVKRFTSLKSLYLSNNRFSGEIPADAFLGMPLLK 165

Query: 218 KVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLKQASITSLDFSNNKLEGEIP 277
           K+ L+ N F G IP SL  L  L+EL L  NQF G IP  +Q  +    F NN L+G IP
Sbjct: 166 KILLANNAFRGTIPSSLASLPMLLELRLNGNQFQGQIPSFQQKDLKLASFENNDLDGPIP 225

Query: 278 ESLSRFEAKAFAGNEALCGKPLPKSCDNNARIS-------EEKPPPSPPGESQGNVSKL- 337
           ESL   +  +FAGN+ LC  PL     ++  +        + K    P G+  G+   L 
Sbjct: 226 ESLRNMDPGSFAGNKGLCDAPLSPCSSSSPGVPVVPVSPVDPKSTSPPTGKKAGSFYTLA 285

Query: 338 VVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVPHDKGGREQSSRRG-G 397
           ++  +I +++ ++  +F     RR +  S       +E +E +  H    +         
Sbjct: 286 IILIVIGIILVIIALVFCFVQSRRRNFLSAYPSSAGKERIESYNYHQSTNKNNKPAESVN 345

Query: 398 DSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANGLSVVV 457
            +RRGS    G    L+ V DD   F L DL++A+AEVLG+G  G++YKA +++G ++VV
Sbjct: 346 HTRRGSMPDPGGR--LLFVRDDIQRFDLQDLLRASAEVLGSGTFGASYKAAISSGQTLVV 405

Query: 458 KRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHG 517
           KR + MN +G+D F   MRRLG+L H NIL  +AY+YRREEKLLV+E++P  SL   LH 
Sbjct: 406 KRYKHMNNVGRDEFHEHMRRLGRLNHPNILPLVAYYYRREEKLLVTEFMPNSSLASHLHA 465

Query: 518 DRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEPLLSDY 577
           +     A L+W TRL+I+KG+ +G+ +L  E  T  +PHG++KSSN++L D++EPLL+DY
Sbjct: 466 NNS---AGLDWITRLKIIKGVAKGLSYLFDELPTLTIPHGHMKSSNIVLDDSFEPLLTDY 525

Query: 578 AFHPLINPNNATQAMFAYRSPEY--TQYQELSPKSDVYCLGIIILEIMTSKFPSQYLSNG 637
           A  P+++  +A   M AY+SPEY  ++ Q ++ K+DV+C G++ILE++T +FP  YL+ G
Sbjct: 526 ALRPMMSSEHAHNFMTAYKSPEYRPSKGQIITKKTDVWCFGVLILEVLTGRFPENYLTQG 585

Query: 638 -KGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRPEMR 690
                 +V WV+  V EK+  ++ D E+         M+ LL+IG  C     ++R +MR
Sbjct: 586 YDSNMSLVTWVNDMVKEKKTGDVFDKEMKGKKNCKAEMINLLKIGLRCCEEEEERRMDMR 645

BLAST of Spg003446 vs. ExPASy TrEMBL
Match: A0A1S3CB95 (pollen receptor-like kinase 3 OS=Cucumis melo OX=3656 GN=LOC103498999 PE=4 SV=1)

HSP 1 Score: 1058.1 bits (2735), Expect = 1.5e-305
Identity = 542/629 (86.17%), Postives = 574/629 (91.26%), Query Frame = 0

Query: 65  YSIFRSGSAMAAVRIVFLFLIFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSV 124
           +SIF  GSAM AVRIVFL ++   GGLPL C L+ENEALLKLK+SF HSESLNSW  DSV
Sbjct: 44  FSIFPFGSAMGAVRIVFLIILVGGGGLPLCCPLSENEALLKLKESFTHSESLNSWNPDSV 103

Query: 125 PCSARWTGIICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNR 184
           PCSA+WTGIICNRGVITGLHL+ LQLSGKIDVEALL+LRGLRTISFVNN FSGPIPE N+
Sbjct: 104 PCSAQWTGIICNRGVITGLHLSDLQLSGKIDVEALLQLRGLRTISFVNNKFSGPIPEFNK 163

Query: 185 IGVLKSMLLSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLEN 244
           IGVLKS+LL+GN FSG IP+DFFSSLTSLKKVWLS N FSGNIPHSL QLSHLIELHLE+
Sbjct: 164 IGVLKSLLLTGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLES 223

Query: 245 NQFSGPIPHLKQAS-ITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNN 304
           NQFSGPIPHLK AS ITSLD SNNKLEG+IP+ LS+F+AKAFAGNE LCG PLPKSC   
Sbjct: 224 NQFSGPIPHLKHASIITSLDLSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSC--G 283

Query: 305 ARISE-EKPPPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHM 364
           ARI E +KP  SPPGESQGNVSKLVVASLIAV VFLMVFIFLSASKRREDEFSVLGRE M
Sbjct: 284 ARIPEDQKPSSSPPGESQGNVSKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGRERM 343

Query: 365 EEVVEVHVPHDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAE 424
           EEVVEVHVP     ++ S + GGDS+RGSQQGK  MSDLVVVN+DKG FGLADLMKAAAE
Sbjct: 344 EEVVEVHVPSSGHDKQSSRKGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAE 403

Query: 425 VLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHY 484
           VLGNGGLGSAYKAVM+NGLSVVVKRMREMNKLGKDGFDAEMRRLG+LRH NILTPLAYHY
Sbjct: 404 VLGNGGLGSAYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHY 463

Query: 485 RREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDV 544
           RREEKLLVSEYIPKGSLLYVLHGDRGACHA+LNW TRLRIV+GI RG+GFLHSEFATYD+
Sbjct: 464 RREEKLLVSEYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDL 523

Query: 545 PHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYC 604
           PHGNLKSSNVLL DNYEPLLSDYAFHPLINPNNATQAMFAYRSPEY QYQE+SPKSDVYC
Sbjct: 524 PHGNLKSSNVLLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYC 583

Query: 605 LGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQ 664
           LGIIILEIMTSKFPSQYL+NGKGGTDVVQWVSSAVSEKREAELIDPEIANDT ALDRMV 
Sbjct: 584 LGIIILEIMTSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVN 643

Query: 665 LLRIGADCTLSNPQQRPEMREAIRRIEEI 692
           LL IGADCT +NPQQRPEMREAIRRIEEI
Sbjct: 644 LLTIGADCTHNNPQQRPEMREAIRRIEEI 670

BLAST of Spg003446 vs. ExPASy TrEMBL
Match: A0A5D3CFY4 (Pollen receptor-like kinase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001520 PE=4 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.9e-303
Identity = 537/620 (86.61%), Postives = 568/620 (91.61%), Query Frame = 0

Query: 74  MAAVRIVFLFLIFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTGI 133
           M AVRIVFL ++   GGLPL C L+ENEALLKLK+SF HSESLNSW  DSVPCSA+WTGI
Sbjct: 1   MGAVRIVFLIILVGGGGLPLCCPLSENEALLKLKESFTHSESLNSWNPDSVPCSAQWTGI 60

Query: 134 ICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLL 193
           ICNRGVITGLHL+ LQLSGKIDVEALL+LRGLRTISFVNN FSGPIPE N+IGVLKS+LL
Sbjct: 61  ICNRGVITGLHLSDLQLSGKIDVEALLQLRGLRTISFVNNKFSGPIPEFNKIGVLKSLLL 120

Query: 194 SGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPH 253
           +GN FSG IP+DFFSSLTSLKKVWLS N FSGNIPHSL QLSHLIELHLE+NQFSGPIPH
Sbjct: 121 TGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLESNQFSGPIPH 180

Query: 254 LKQAS-ITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNNARISE-EKP 313
           LK AS ITSLD SNNKLEG+IP+ LS+F+AKAFAGNE LCG PLPKSC   ARI E +KP
Sbjct: 181 LKHASIITSLDLSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSC--GARIPEDQKP 240

Query: 314 PPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVP 373
           P SPPGESQGNVSKLVVASLIAV VFLMVFIFLSASKRREDEFSVLGRE MEEVVEVHVP
Sbjct: 241 PSSPPGESQGNVSKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGREQMEEVVEVHVP 300

Query: 374 HDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGS 433
                ++ S + GGDS+RGSQQGK  MSDLVVVN+DKG FGLADLMKAAAEVLGNGGLGS
Sbjct: 301 SSGHDKQSSRKGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAEVLGNGGLGS 360

Query: 434 AYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVS 493
           AYKAVM+NGLSVVVKRMREMNKLGKDGFDAEMRRLG+LRH NILTPLAYHYRREEKLLVS
Sbjct: 361 AYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHYRREEKLLVS 420

Query: 494 EYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSN 553
           EYIPKGSLLYVLHGDRGACHA+LNW TRLRIV+GI RG+GFLHSEFATYD+PHGNLKSSN
Sbjct: 421 EYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDLPHGNLKSSN 480

Query: 554 VLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIM 613
           VLL DNYEPLLSDYAFHPLINPNNATQAMFAYRSPEY QYQE+SPKSDVYCLGIIILEIM
Sbjct: 481 VLLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYCLGIIILEIM 540

Query: 614 TSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCT 673
           TSKFPSQYL+NGKGGTDVVQWVSSAVSEKREAELIDPEIANDT ALDRMV LL IGADCT
Sbjct: 541 TSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVNLLTIGADCT 600

Query: 674 LSNPQQRPEMREAIRRIEEI 692
            +NPQQRPEMREAIRRIEEI
Sbjct: 601 HNNPQQRPEMREAIRRIEEI 618

BLAST of Spg003446 vs. ExPASy TrEMBL
Match: A0A0A0LIJ2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000010 PE=4 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 2.1e-302
Identity = 535/620 (86.29%), Postives = 568/620 (91.61%), Query Frame = 0

Query: 74  MAAVRIVFLFLIFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTGI 133
           M AVRIVFL ++   GGLP  C L+ENEALLKLK+SF HSESLNSW  DSVPCSARW GI
Sbjct: 1   MGAVRIVFLIILVGGGGLPSCCPLSENEALLKLKESFTHSESLNSWNPDSVPCSARWIGI 60

Query: 134 ICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLL 193
           ICNRGVITGLHL+GLQLSGKIDVEALL+LRGLRTISFV+N FSGPIPE N+IGVLKS+LL
Sbjct: 61  ICNRGVITGLHLSGLQLSGKIDVEALLQLRGLRTISFVDNQFSGPIPEFNKIGVLKSLLL 120

Query: 194 SGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPH 253
           +GN FSG IP+DFFSSLTSLKKVWLS N FSGNIPHSL QLSHLIELHLE+NQFSGPIPH
Sbjct: 121 TGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLESNQFSGPIPH 180

Query: 254 LKQAS-ITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNNARISE-EKP 313
           LK AS ITSL+ SNNKLEG+IP+ LS+F+AKAFAGNE LCG PLPKSC   A+ISE +KP
Sbjct: 181 LKHASIITSLNVSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSC--GAQISEDQKP 240

Query: 314 PPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVP 373
           P SPPGESQGN+SKLVVASLIAV VFLMVFIFLSASKRREDEFSVLGRE MEEVVEVHVP
Sbjct: 241 PSSPPGESQGNISKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGREQMEEVVEVHVP 300

Query: 374 HDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGS 433
                ++ S R GGDS+RGSQQGK  MSDLVVVN+DKG FGLADLMKAAAEVLGNGGLGS
Sbjct: 301 SSGHDKQSSRRGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAEVLGNGGLGS 360

Query: 434 AYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVS 493
           AYKAVM+NGLSVVVKRMREMNKLGKDGFDAEMRRLG+LRH NILTPLAYHYRREEKLLVS
Sbjct: 361 AYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHYRREEKLLVS 420

Query: 494 EYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSN 553
           EYIPKGSLLYVLHGDRGACHA+LNW TRLRIV+GI RG+GFLHSEFATYD+PHGNLKSSN
Sbjct: 421 EYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDLPHGNLKSSN 480

Query: 554 VLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIM 613
           VLL DNYEPLLSDYAFHPLINPNNATQAMFAYRSPEY QYQE+SPKSDVYCLGIIILEIM
Sbjct: 481 VLLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYCLGIIILEIM 540

Query: 614 TSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCT 673
           TSKFPSQYL+NGKGGTDVVQWVSSAVSEKREAELIDPEIANDT ALDRMV LL IGADCT
Sbjct: 541 TSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVHLLTIGADCT 600

Query: 674 LSNPQQRPEMREAIRRIEEI 692
            +NPQQRPEMREAIRRIEEI
Sbjct: 601 HNNPQQRPEMREAIRRIEEI 618

BLAST of Spg003446 vs. ExPASy TrEMBL
Match: A0A6J1FAK5 (pollen receptor-like kinase 3 OS=Cucurbita moschata OX=3662 GN=LOC111443910 PE=4 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 2.0e-297
Identity = 521/620 (84.03%), Postives = 572/620 (92.26%), Query Frame = 0

Query: 74  MAAVRIVFLFL-IFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTG 133
           MAAVR VFLFL IFL+GG PLSCSL++NEALLKL+QSF H+ESLNSW  DS PC+ARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWNQDSQPCAARWTG 60

Query: 134 IICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSML 193
           IIC+RGVITGLHLTGL+LSG+ID+EALLELR LRT+S VNNSF+GPIPE NRIGVLKS+L
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLELRSLRTVSLVNNSFAGPIPEFNRIGVLKSLL 120

Query: 194 LSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIP 253
           L+GNDFSG IP DFF+SL SLKKVWLS N FSG+IPHSL +LS+LIELHLENNQFSG IP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGKIP 180

Query: 254 HLKQASITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCD-NNARISEEKP 313
           HL QA++TSLD SNNKLEGEIPE+L++F+A AFAGNE LCGKPLPKSCD NNA+ISEE  
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDSNNAQISEEN- 240

Query: 314 PPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVP 373
             SPPGESQG+VSKLVVASLIAV VFL+V +FLSASKRRE++FS LGRE M E+VEVHVP
Sbjct: 241 -ASPPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVP 300

Query: 374 HDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGS 433
              G REQ SR+GGDSRRGSQQGKG MSDLVVVN++KGTFG+ADLMKAAAEVLGNGGLGS
Sbjct: 301 -SSGHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGS 360

Query: 434 AYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVS 493
           AYKAVMANGLSVVVKR+REMNKLGKDGFDAEMRR+G+L+H NILTPLAYHYRREEKLLVS
Sbjct: 361 AYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVS 420

Query: 494 EYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSN 553
           EYIPKGSLLYVLHGDRGACHAELNW TRL+IVKGI RG+GFLHSEFATYD+PHGNLKSSN
Sbjct: 421 EYIPKGSLLYVLHGDRGACHAELNWATRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSN 480

Query: 554 VLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIM 613
           +LLSD YEPLLSDYAFHPLINPNNAT+AMFAY+SPEY Q QE+SPKSDVYCLGIIILEI+
Sbjct: 481 ILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAQRQEVSPKSDVYCLGIIILEIL 540

Query: 614 TSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCT 673
           T KFPSQYLSNGKGGTDVVQWVSSAV+EKREAELIDPEIAND  A+ RMV+LLRIGADCT
Sbjct: 541 TGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCT 600

Query: 674 LSNPQQRPEMREAIRRIEEI 692
            SNPQQRPEM+EAIRRIEEI
Sbjct: 601 QSNPQQRPEMKEAIRRIEEI 617

BLAST of Spg003446 vs. ExPASy TrEMBL
Match: A0A6J1HQR8 (pollen receptor-like kinase 3 OS=Cucurbita maxima OX=3661 GN=LOC111465780 PE=4 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 5.5e-295
Identity = 517/620 (83.39%), Postives = 570/620 (91.94%), Query Frame = 0

Query: 74  MAAVRIVFLFL-IFLLGGLPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPCSARWTG 133
           MAAVR VFLFL IFL+GG PLSCSL++NEALLKL+QSF H+ESLNSW  DS PC+ARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60

Query: 134 IICNRGVITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSML 193
           IIC+RGVITGLHLTGL+LSG+ID+EALL+LR LRT+S VNNSF+GPIPE N+IGVLKS+L
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120

Query: 194 LSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIP 253
           L+GNDFSG IP DFF+SL SLKKVWLS N FSG+IPHSL +LS+LIELHLENNQFSG IP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180

Query: 254 HLKQASITSLDFSNNKLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCD-NNARISEEKP 313
           HL QA++TSLD SNNKLEGEIPE+L++F+A AFAGNE LCGKPLPKSCD NNA+ISEE  
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEEN- 240

Query: 314 PPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEVVEVHVP 373
             S PGESQG+VSKLVVASLIAV VFL+V +FLSASKRRE++FS LGRE M E+VEVHVP
Sbjct: 241 -ASSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVP 300

Query: 374 HDKGGREQSSRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGS 433
              G REQ SR+GGDSRRGSQQGKG MSDLVVVN++KGTFG+ADLMKAAAEVLGNGGLGS
Sbjct: 301 -SSGHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGS 360

Query: 434 AYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVS 493
           AYKAVMANGLSVVVKR+REMNKLGKDGFDAEMRR+G+L+H NILTPLAYHYRREEKLLVS
Sbjct: 361 AYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVS 420

Query: 494 EYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSN 553
           EYIPKGSLLYVLHGDRGACHAELNWGTRL+IVKGI RG+GFLHSEFATYD+PHGNLKSSN
Sbjct: 421 EYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSN 480

Query: 554 VLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIM 613
           +LLSD YEPLLSDYAFHPLINPNNAT+AMFAY+SPEY   QE+SPKSDVYCLGIIILEI+
Sbjct: 481 ILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEIL 540

Query: 614 TSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCT 673
           T KFPSQYLSNGKGGTDVVQWVSSAV+EKREAELIDPEIAND  A+ RMV+LLRIGADCT
Sbjct: 541 TGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCT 600

Query: 674 LSNPQQRPEMREAIRRIEEI 692
            SNPQQRPEM+EAIRRI EI
Sbjct: 601 QSNPQQRPEMKEAIRRIXEI 617

BLAST of Spg003446 vs. TAIR 10
Match: AT3G42880.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 654.8 bits (1688), Expect = 7.6e-188
Identity = 344/640 (53.75%), Postives = 458/640 (71.56%), Query Frame = 0

Query: 79  IVFL-FLIFLLGGLPLSCSLTENEALLKLKQSFAHSE-SLNSWTSDSVPCSARWTGIICN 138
           ++FL FL+      P   +++E+E L++ K+S   ++  LNSW + + PC+ +W GI C 
Sbjct: 4   VLFLCFLLICFSFTPSLQNVSESEPLVRFKRSVNITKGDLNSWRTGTDPCNGKWFGIYCQ 63

Query: 139 RG-VITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSG 198
           +G  ++G+H+T L LSG I++E L +L  LRTI   NN  SGP+P   ++  LKS+LLS 
Sbjct: 64  KGQTVSGIHVTRLGLSGTINIEDLKDLPNLRTIRLDNNLLSGPLPPFFKLPGLKSLLLSN 123

Query: 199 NDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLK 258
           N FSG I +DFF     LK+V+L  N+ SG IP SL QL+ L ELH++ NQF+G IP L 
Sbjct: 124 NSFSGEIADDFFKETPQLKRVFLDNNRLSGKIPASLMQLAGLEELHMQGNQFTGEIPPLT 183

Query: 259 QAS--ITSLDFSNNKLEGEIPESLS---RFEAKAFAGNEALCGKPLPKSCDNNARISEEK 318
             +  + SLD SNN LEGEIP ++S     E K F GN+ LCG PL   CD       EK
Sbjct: 184 DGNKVLKSLDLSNNDLEGEIPITISDRKNLEMK-FEGNQRLCGSPLNIECD-------EK 243

Query: 319 PPPSPPGESQGNVSKLVVASLIAVVVFLMVFIFLSA-----SKRREDEFSVLGREHM--E 378
           P  +  G  + N +K    ++  V++FL++F+F+ A      K+R+ EF +LG++H+  +
Sbjct: 244 PSSTGSGNEKNNTAK----AIFMVILFLLIFLFVVAIITRWKKKRQPEFRMLGKDHLSDQ 303

Query: 379 EVVEVHVPHD-----KGGREQSSRRGGDSRRGSQQGKGA-------MSDLVVVNDDKGTF 438
           E VEV VP          +++S+  G   +  S  GKGA       M D+++VN +KG+F
Sbjct: 304 ESVEVRVPDSIKKPIDSSKKRSNAEGSSKKGSSHNGKGAGGGPGSGMGDIIMVNSEKGSF 363

Query: 439 GLADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRH 498
           GL DLMKAAAEVLGNG LGSAYKAVMANGLSVVVKR+R+MNKL ++ FD EM+R GKLRH
Sbjct: 364 GLPDLMKAAAEVLGNGSLGSAYKAVMANGLSVVVKRIRDMNKLAREAFDTEMQRFGKLRH 423

Query: 499 QNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMG 558
            N+LTPLAYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL W TRL+I++G+ RGM 
Sbjct: 424 PNVLTPLAYHYRREEKLVVSEYMPKSSLLYVLHGDRGVYHSELTWATRLKIIQGVARGMD 483

Query: 559 FLHSEFATYDVPHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQY 618
           FLH EFA+YD+PHGNLKSSNVLLS+ YEPL+SDYAF PL+ PNNA+QA+FA++SPE+ Q 
Sbjct: 484 FLHEEFASYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPNNASQALFAFKSPEFVQN 543

Query: 619 QELSPKSDVYCLGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSAVSEKREAELIDPEIA 678
           Q++SPKSDVYCLGII+LE+MT KFPSQYL+ GKGGTD+V+WV S++++ +E ELIDPEIA
Sbjct: 544 QQVSPKSDVYCLGIIVLEVMTGKFPSQYLNTGKGGTDIVEWVQSSIAQHKEEELIDPEIA 603

Query: 679 NDTGALDRMVQLLRIGADCTLSNPQQRPEMREAIRRIEEI 692
           ++T ++ +MV+LLRIGA C  SNP +R  M+E +RRIE +
Sbjct: 604 SNTDSIKQMVELLRIGAACIASNPNERQNMKEIVRRIERV 631

BLAST of Spg003446 vs. TAIR 10
Match: AT5G20690.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 616.3 bits (1588), Expect = 3.0e-176
Identity = 332/655 (50.69%), Postives = 449/655 (68.55%), Query Frame = 0

Query: 75  AAVRIVFLFLIFLLGGLPLSCSL---TENEALLKLKQSFAHSE-SLNSWTSDSVPCSARW 134
           AAV     FL+ LL    +S SL   +E+E L++ K S   ++  LNSW   + PCS +W
Sbjct: 3   AAVLNPGFFLLILLLSFSISPSLQYVSESEPLVRFKNSVKITKGDLNSWREGTDPCSGKW 62

Query: 135 TGIICNRGV-ITGLHLTGLQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLK 194
            GI C +G+ ++G+H+T L LSG I V+ L +L  L+TI   NN  SGP+P   ++  LK
Sbjct: 63  FGIYCQKGLTVSGIHVTRLGLSGTITVDDLKDLPNLKTIRLDNNLLSGPLPHFFKLRGLK 122

Query: 195 SMLLSGNDFSGVIPNDFFSSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSG 254
           S++LS N FSG I +DFF  ++ LK+++L  NKF G+IP S+ QL  L ELH+++N  +G
Sbjct: 123 SLMLSNNSFSGEIRDDFFKDMSKLKRLFLDHNKFEGSIPSSITQLPQLEELHMQSNNLTG 182

Query: 255 PIP--HLKQASITSLDFSNNKLEGEIPESLS--RFEAKAFAGNEALCGKPLPKSCDN-NA 314
            IP       ++  LD S N L+G +P+S++  +  A     NE LCG  +   C+N   
Sbjct: 183 EIPPEFGSMKNLKVLDLSTNSLDGIVPQSIADKKNLAVNLTENEYLCGPVVDVGCENIEL 242

Query: 315 RISEEKPPPSPPGES------QGNVSKLVVASLIAVVVFLMVFIFLSASKRREDEFSVLG 374
              +E  PPS P  S      +  ++ ++V+  + ++ F++V +    +K++  +F +L 
Sbjct: 243 NDPQEGQPPSKPSSSVPETSNKAAINAIMVSISLLLLFFIIVGVIKRRNKKKNPDFRMLA 302

Query: 375 REHMEEVVEVHVPHD-----KGGREQSSRRGGDSRRGSQQ------GK-----------G 434
                +VVEV +        K   + S +RGG S  GS +      GK           G
Sbjct: 303 NNRENDVVEVRISESSSTTAKRSTDSSRKRGGHSDDGSTKKGVSNIGKGGNGGGGGALGG 362

Query: 435 AMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRMREMNKLGK 494
            M D+++VN DKG+FGL DLMKAAAEVLGNG LGSAYKAVM  GLSVVVKR+R+MN+L +
Sbjct: 363 GMGDIIMVNTDKGSFGLPDLMKAAAEVLGNGSLGSAYKAVMTTGLSVVVKRIRDMNQLAR 422

Query: 495 DGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNW 554
           + FD EMRR GKLRH NILTPLAYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL W
Sbjct: 423 EPFDVEMRRFGKLRHPNILTPLAYHYRREEKLVVSEYMPKSSLLYVLHGDRGIYHSELTW 482

Query: 555 GTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEPLLSDYAFHPLINPNNA 614
            TRL+I++G+  GM FLH EFA+YD+PHGNLKSSNVLLS+ YEPL+SDYAF PL+ P+NA
Sbjct: 483 ATRLKIIQGVAHGMKFLHEEFASYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPSNA 542

Query: 615 TQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIMTSKFPSQYLSNGKGGTDVVQWVSSA 674
           +QA+FA+++PE+ Q Q++S KSDVYCLGIIILEI+T KFPSQYL+NGKGGTD+VQWV S+
Sbjct: 543 SQALFAFKTPEFAQTQQVSHKSDVYCLGIIILEILTGKFPSQYLNNGKGGTDIVQWVQSS 602

Query: 675 VSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRPEMREAIRRIEEI 692
           V+E++E ELIDPEI N+T ++ +MV+LLR+GA C  SNP +R +MREA+RRIE++
Sbjct: 603 VAEQKEEELIDPEIVNNTESMRQMVELLRVGAACIASNPDERLDMREAVRRIEQV 657

BLAST of Spg003446 vs. TAIR 10
Match: AT1G72460.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-142
Identity = 279/619 (45.07%), Postives = 397/619 (64.14%), Query Frame = 0

Query: 96  SLTENEALLKLKQSFAHSESLNSWTSDSVPCSA--RWTGIICNRGVITGLHLTGLQLSGK 155
           S+TE+E+LLK K+S  +++SL+SWT +S PC A  RW G++CN+  + GL +  + LSGK
Sbjct: 21  SITESESLLKFKKSLNNTKSLDSWTPESEPCGASQRWIGLLCNKNSVFGLQIEQMGLSGK 80

Query: 156 IDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSGNDFSGVIPNDFFSSLTSL 215
           +DV  L +L  LRTIS +NNSFSG IPE NR+  LKS+ +SGN FSG IP+D+F ++ SL
Sbjct: 81  VDVAPLKDLPSLRTISIMNNSFSGDIPEFNRLTALKSLYISGNRFSGNIPSDYFETMVSL 140

Query: 216 KKVWLSFNKFSGNIPHSLG-QLSHLIELHLENNQFSGPIPHLKQASITSLDFSNNKLEGE 275
           KK WLS N FSG IP SL   L +LIEL LENNQF G IP+  Q ++  +D SNN+L GE
Sbjct: 141 KKAWLSNNHFSGLIPISLATTLPNLIELRLENNQFIGSIPNFTQTTLAIVDLSNNQLTGE 200

Query: 276 IPESLSRFEAKAFAGNEALCG----KPLPKSCDNNARISEEKPPPSPPGESQGNVSKLVV 335
           IP  L +F+AK FAGN  LCG     P P+  ++ A I+ E            N SK  +
Sbjct: 201 IPPGLLKFDAKTFAGNSGLCGAKLSTPCPQPKNSTASITIEGT------MKDANKSKYFL 260

Query: 336 ASLIAVVVFLMVFIFLSASKRREDEFSVLGREHME------EVVEVHVPHDKGGREQSSR 395
           A     V+ ++V + L+  K+++       R   E      + ++V V      R+  S 
Sbjct: 261 AFSTLGVLLIVVLVSLAFRKKKKKRRRKKARRTSEQDNSDDQQIQVTVEGSNSSRQSRSS 320

Query: 396 RGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGN-----------GGLGS 455
           R G+  +    G    SDLV+VN +KG F L+DLMKAAA VLGN           GG+GS
Sbjct: 321 RSGELNK----GVAGTSDLVMVNKEKGVFRLSDLMKAAAHVLGNPGGGSNRPRSSGGVGS 380

Query: 456 AYKAVMANGLSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVS 515
           AYKAV++NG++VVVKR+  MN++  D FD E+R+LG L+H+N+LTPLAYH+R++EKLLV 
Sbjct: 381 AYKAVLSNGVTVVVKRVTVMNQVSVDVFDKEIRKLGSLQHKNVLTPLAYHFRQDEKLLVF 440

Query: 516 EYIPKGSLLYVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSN 575
           E++P  +LL+ LHGD      +L+W +RL+I++GI RGM +LH E    ++PHGNLKSSN
Sbjct: 441 EFVPNLNLLHRLHGDHE--EFQLDWPSRLKIIQGIARGMWYLHRELGFLNLPHGNLKSSN 500

Query: 576 VLLSDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIM 635
           + L+++ EPL+S++    LINP+  +Q++ A++SPE  +   +S KSDV+  G+++LEI+
Sbjct: 501 IFLAEDGEPLISEFGLQKLINPDAQSQSLVAFKSPEADRDGTVSAKSDVFSFGVVVLEIL 560

Query: 636 TSKFPSQYLS-NGKGGTDVVQWVSSAVSEKREAELIDPEI----ANDTGALDRMVQLLRI 686
           T KFPSQY   N  GG ++V+W+ SA+ +    +L+ P +    A D    + +  +LRI
Sbjct: 561 TGKFPSQYAGLNRAGGANLVEWLGSALEQGGWMDLLHPMVVTAAAEDKIMEEEIENVLRI 620

BLAST of Spg003446 vs. TAIR 10
Match: AT3G20190.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 452.2 bits (1162), Expect = 7.4e-127
Identity = 245/612 (40.03%), Postives = 370/612 (60.46%), Query Frame = 0

Query: 91  LPLSCSLTENEALLKLKQSFAHSESLNSWTSDSVPC---SARWTGIICNRGVITGLHLTG 150
           LPL  S  + + LL+ K +  ++  ++SW     PC   S  W G++C  G + GL L G
Sbjct: 46  LPLPAS--DADCLLRFKDTLVNASFISSWDPSISPCKRNSENWFGVLCVTGNVWGLQLEG 105

Query: 151 LQLSGKIDVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSGNDFSGVIPNDFF 210
           + L+GK+D+E L  ++ LRT+SF+NN F+G +P +   G LKS+ LS N F+G IP D F
Sbjct: 106 MGLTGKLDLEPLAAIKNLRTLSFMNNKFNGSMPSVKNFGALKSLYLSNNRFTGEIPADAF 165

Query: 211 SSLTSLKKVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLKQASITSLDFSNN 270
             +  LKK+ L+ N F G+IP SL  L  L+EL L  NQF G IP+ KQ  +    F NN
Sbjct: 166 DGMHHLKKLLLANNAFRGSIPSSLAYLPMLLELRLNGNQFHGEIPYFKQKDLKLASFENN 225

Query: 271 KLEGEIPESLSRFEAKAFAGNEALCGKPLPKSCDNNARISEEKPPPSPPGESQGNVS--- 330
            LEG IPESLS  +  +F+GN+ LCG PL   C +++  S +   PS P E   N S   
Sbjct: 226 DLEGPIPESLSNMDPVSFSGNKNLCGPPL-SPCSSDSGSSPDL--PSSPTEKNKNQSFFI 285

Query: 331 ---KLVVASLIAVVVFLMVFIFLSASKRREDEFSVLGREHMEEV-VEVHVPHDKGGREQS 390
               L+V  +I +++ L+V I  +  ++    +   G++  E+   +     DK     +
Sbjct: 286 IAIVLIVIGIILMIISLVVCILHTRRRKSLSAYPSAGQDRTEKYNYDQSTDKDKAADSVT 345

Query: 391 SRRGGDSRRGSQQGKGAMSDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANG 450
           S     SRRG+   +   + L+ + DD   F L DL++A+AEVLG+G  GS+YK  + +G
Sbjct: 346 SY---TSRRGAVPDQ---NKLLFLQDDIQRFDLQDLLRASAEVLGSGSFGSSYKTGINSG 405

Query: 451 LSVVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLL 510
             +VVKR + MN +G+D F   MRRLG+L+H N+L  +AY+YRREEKLL++E++P  SL 
Sbjct: 406 QMLVVKRYKHMNNVGRDEFHEHMRRLGRLKHPNLLPIVAYYYRREEKLLIAEFMPNRSLA 465

Query: 511 YVLHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEP 570
             LH +       L+W TRL+I++G+ +G+G+L +E  T  +PHG+LKSSNV+L +++EP
Sbjct: 466 SHLHANHSVDQPGLDWPTRLKIIQGVAKGLGYLFNELTTLTIPHGHLKSSNVVLDESFEP 525

Query: 571 LLSDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIMTSKFPSQYL 630
           LL+DYA  P++N   +   M +Y+SPEY+    L+ K+DV+CLG++ILE++T +FP  YL
Sbjct: 526 LLTDYALRPVMNSEQSHNLMISYKSPEYSLKGHLTKKTDVWCLGVLILELLTGRFPENYL 585

Query: 631 SNG-KGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRP 690
           S G      +V WVS+ V EK+  ++ D E+         M+ LL+IG  C   + ++R 
Sbjct: 586 SQGYDANMSLVTWVSNMVKEKKTGDVFDKEMTGKKNCKAEMLNLLKIGLSCCEEDEERRM 645

Query: 691 EMREAIRRIEEI 692
           EMR+A+ +IE +
Sbjct: 646 EMRDAVEKIERL 646

BLAST of Spg003446 vs. TAIR 10
Match: AT5G35390.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 435.3 bits (1118), Expect = 9.4e-122
Identity = 245/609 (40.23%), Postives = 373/609 (61.25%), Query Frame = 0

Query: 97  LTENEALLKLKQSF--AHSESLNSWTSDSVPCSARWTGIICNRGVITGLHLTGLQLSGKI 156
           L+++EA+LK K+S       +L SW + S PC+  W+G++CN G +  L +  L+LSG I
Sbjct: 32  LSDSEAILKFKESLVVGQENALASWNAKSPPCT--WSGVLCNGGSVWRLQMENLELSGSI 91

Query: 157 DVEALLELRGLRTISFVNNSFSGPIPELNRIGVLKSMLLSGNDFSGVIPNDFFSSLTSLK 216
           D+EAL  L  LRT+SF+NN F GP P+  ++  LKS+ LS N F G IP D F  +  LK
Sbjct: 92  DIEALSGLTSLRTLSFMNNKFEGPFPDFKKLAALKSLYLSNNQFGGDIPGDAFEGMGWLK 151

Query: 217 KVWLSFNKFSGNIPHSLGQLSHLIELHLENNQFSGPIPHLKQASITSLDFSNNKLEGEIP 276
           KV L+ NKF+G IP S+ +L  L+EL L+ NQF+G IP  +   +  L+ SNN L G IP
Sbjct: 152 KVHLAQNKFTGQIPSSVAKLPKLLELRLDGNQFTGEIPEFEH-QLHLLNLSNNALTGPIP 211

Query: 277 ESLSRFEAKAFAGNEALCGKPLPKSCDNNARISEEKPPPS---PPGESQGN-VSKLVVAS 336
           ESLS  + K F GN+ L GKPL   CD+      E PP S   P   S+G  V   +VA+
Sbjct: 212 ESLSMTDPKVFEGNKGLYGKPLETECDSP---YIEHPPQSEARPKSSSRGPLVITAIVAA 271

Query: 337 LIAVVVFLMVFIFLSASKRREDEFSV-LGREHMEEVVEVHVPHDKGGREQSSRRGGDSRR 396
           L  +++  ++F+   + K ++   +V  G   +++   +     +  + +  R+  D R+
Sbjct: 272 LTILIILGVIFLLNRSYKNKKPRLAVETGPSSLQKKTGIR----EADQSRRDRKKADHRK 331

Query: 397 GSQQGK--GAM-----SDLVVVNDDKGTFGLADLMKAAAEVLGNGGLGSAYKAVMANGLS 456
           GS   K  GA      + L  + +D+  F L DL+KA+AE+LG+G  G++YKAV+++G  
Sbjct: 332 GSGTTKRMGAAAGVENTKLSFLREDREKFDLQDLLKASAEILGSGCFGASYKAVLSSGQM 391

Query: 457 VVVKRMREMNKLGKDGFDAEMRRLGKLRHQNILTPLAYHYRREEKLLVSEYIPKGSLLYV 516
           +VVKR ++MN  G+D F   M+RLG+L H N+L+ +AY+YR+EEKLLV ++  +GSL   
Sbjct: 392 MVVKRFKQMNNAGRDEFQEHMKRLGRLMHHNLLSIVAYYYRKEEKLLVCDFAERGSLAIN 451

Query: 517 LHGDRGACHAELNWGTRLRIVKGIGRGMGFLHSEFATYDVPHGNLKSSNVLLSDNYEPLL 576
           LH ++      L+W TRL+IVKG+ +G+ +LH +  +   PHG+LKSSNVLL+  +EPLL
Sbjct: 452 LHSNQSLGKPSLDWPTRLKIVKGVAKGLFYLHQDLPSLMAPHGHLKSSNVLLTKTFEPLL 511

Query: 577 SDYAFHPLINPNNATQAMFAYRSPEYTQYQELSPKSDVYCLGIIILEIMTSKFPSQYLSN 636
           +DY   PLIN   A   M AYRSPEY Q++ ++ K+DV+ LGI+ILEI+T KFP+ +  +
Sbjct: 512 TDYGLIPLINQEKAQMHMAAYRSPEYLQHRRITKKTDVWGLGILILEILTGKFPANFSQS 571

Query: 637 GKGGTDVVQWVSSAVSEKREAELIDPEIANDTGALDRMVQLLRIGADCTLSNPQQRPEMR 692
            +   D+  WV+S         L D  +   +    ++++LL IG +C   + ++R ++ 
Sbjct: 572 SE--EDLASWVNSGFHGVWAPSLFDKGMGKTSHCEGQILKLLTIGLNCCEPDVEKRLDIG 628

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460083.13.2e-30586.17PREDICTED: pollen receptor-like kinase 3 [Cucumis melo][more]
XP_004141181.24.2e-30586.01pollen receptor-like kinase 3 [Cucumis sativus][more]
KAA0061448.13.9e-30386.61pollen receptor-like kinase 3 [Cucumis melo var. makuwa] >TYK10833.1 pollen rece... [more]
XP_038889222.11.3e-30186.63pollen receptor-like kinase 3 [Benincasa hispida][more]
XP_023537808.19.9e-29984.52pollen receptor-like kinase 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M1L71.1e-18653.75Pollen receptor-like kinase 3 OS=Arabidopsis thaliana OX=3702 GN=PRK3 PE=1 SV=1[more]
Q3E9914.2e-17550.69Pollen receptor-like kinase 6 OS=Arabidopsis thaliana OX=3702 GN=PRK6 PE=1 SV=4[more]
Q9LJY01.0e-12540.03Pollen receptor-like kinase 4 OS=Arabidopsis thaliana OX=3702 GN=PRK4 PE=1 SV=1[more]
C0LGU01.3e-12040.23Pollen receptor-like kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PRK1 PE=1 SV=1[more]
Q9LPT11.7e-12038.55Pollen receptor-like kinase 5 OS=Arabidopsis thaliana OX=3702 GN=PRK5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CB951.5e-30586.17pollen receptor-like kinase 3 OS=Cucumis melo OX=3656 GN=LOC103498999 PE=4 SV=1[more]
A0A5D3CFY41.9e-30386.61Pollen receptor-like kinase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A0A0LIJ22.1e-30286.29Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000... [more]
A0A6J1FAK52.0e-29784.03pollen receptor-like kinase 3 OS=Cucurbita moschata OX=3662 GN=LOC111443910 PE=4... [more]
A0A6J1HQR85.5e-29583.39pollen receptor-like kinase 3 OS=Cucurbita maxima OX=3661 GN=LOC111465780 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G42880.17.6e-18853.75Leucine-rich repeat protein kinase family protein [more]
AT5G20690.13.0e-17650.69Leucine-rich repeat protein kinase family protein [more]
AT1G72460.11.6e-14245.07Leucine-rich repeat protein kinase family protein [more]
AT3G20190.17.4e-12740.03Leucine-rich repeat protein kinase family protein [more]
AT5G35390.19.4e-12240.23Leucine-rich repeat protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 99..136
e-value: 2.0E-8
score: 34.4
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 258..298
e-value: 1.1E-7
score: 32.4
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 97..255
e-value: 3.5E-34
score: 119.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 494..691
e-value: 7.8E-37
score: 128.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 386..493
e-value: 6.3E-16
score: 60.1
NoneNo IPR availablePIRSRPIRSR000550-2PIRSR000550-2coord: 423..616
e-value: 2.9E-4
score: 16.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..321
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..389
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..398
NoneNo IPR availablePANTHERPTHR48007LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1coord: 79..691
NoneNo IPR availablePANTHERPTHR48007:SF38LEUCINE-RICH REPEAT PROTEIN KINASE FAMILY PROTEINcoord: 79..691
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 94..279
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 422..685
e-value: 1.1E-26
score: 93.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 418..691
score: 26.336601
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 422..687

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003446.1Spg003446.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009579 thylakoid
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004672 protein kinase activity