Spg003436 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003436 vs. NCBI nr
Match: KAA0047596.1 (protein Ycf2-like [Cucumis melo var. makuwa])

HSP 1 Score: 256.5 bits (654), Expect = 6.9e-64
Identity = 258/845 (30.53%), Postives = 389/845 (46.04%), Query Frame = 0

Query: 9   TRASDRLKAAGVTPGRKPRKQTSPITLGSEQDSGDAMSTSVSVAKGSGEKTKGVKRDRGD 68
           TR SDRL+AAG+T  RK                  ++ T +     S E+   ++    +
Sbjct: 10  TRTSDRLRAAGITGSRK------------------SLPTGIQNLSSSSEER--IEDIMAE 69

Query: 69  GGSGKKVTP-TKKTKVHKRTKKTNDEIEKKP--TGARSNKKKTRAKQTNDTDKASPVTPE 128
           G  GK+ +P T K +V   TKK    ++KK       S K+  R K      K   V  +
Sbjct: 70  GSGGKRESPRTSKKRVRTETKKEEATVQKKQRIKSLVSRKRVYRGK----GKKQKEVEGK 129

Query: 129 IAL-ETSEDTAKNDTEDTESNSVTNDNSSSDDVGEEREK-KKTTIAKKEPPKKQKGGKKG 188
           IA+ E S  +      DT   S            + REK KK    +KE   ++   +KG
Sbjct: 130 IAVGEDSNSSTSEILPDTRKRS------------KAREKGKKVKAVRKEEKAREDRRRKG 189

Query: 189 KKLKTMVEGDTVRVDDDYLMSPSKISKALKINLCCRTEIMDTINTILGDRCRDAFRNTCF 248
           K      + +    D  YLM   + ++ LKINL  ++ +++ I   LGDR  + FR   F
Sbjct: 190 KAPMNPEKSE----DSVYLMCSERRNEPLKINLHIKSTVIEKIKENLGDRLINRFREGIF 249

Query: 249 GHLLDFSFKKTSSQLLLHLIQHQCKPKRTSELYFKIGGKILKFGLREFALITGLNCGPLP 308
           GH L+ S    SSQLLLHLIQ  CKPK TS+L F IGG++L+FGLREFALITGL C  +P
Sbjct: 250 GHFLNLSITNQSSQLLLHLIQRMCKPKSTSQLQFLIGGRVLRFGLREFALITGLRCHEIP 309

Query: 309 QIDTDRLQDSSRFKDEYFANDEGVRRKTLNIVFNAMKHGVEADLVKMAQLFVWRAFC--- 368
            I+ + +    R K  YF N + V R+ LN++FN    G + D +KMA+L+   +F    
Sbjct: 310 DINHEDINGGGRLKGVYFENLKTVTRQYLNVMFNISTTGTDDDRIKMAKLYFLESFLIPK 369

Query: 369 -----------------------------------YLGK---KRGSVGIGMGGLVYAILA 428
                                              ++ +    +G  GI MGG ++ ILA
Sbjct: 370 QECPSVDWDHIIMVDDDEVFDGYPWGRVAFELLVDFMNRVVCSKGQTGISMGGFIFPILA 429

Query: 429 WAYEVIPALSAPPTNYAKRIRNTVPRIINWEVEAQPEWRELHVKIFQSSSLEVVPLDPTD 488
           WAYEVIP LS PP  +  RI N VPRIIN   + QP+W++L  K+F S +LEV P+  T 
Sbjct: 430 WAYEVIPTLSTPPNFFVTRISNEVPRIINCATDTQPKWKDLKQKVFDSPTLEVSPMLATP 489

Query: 489 TEMQMSYFQPFLQDELASRRLAGDEQQVGDDVRIPPNFSV--GAPSMTSQMDVMEKRHQE 548
            E+ M +F PF++ E    + A DE +   +     + S+  G PS TS+++V+ K  ++
Sbjct: 490 DEVGMPFFAPFIETEKDILKEAEDELRKNKNFDHIASVSLNRGMPS-TSEINVLRKMVEK 549

Query: 549 IIGKLDRVYSMLGALVDTLREIHKLDDPPNSKFKMQGDVGTGIDPTTK----------DG 608
           I     R+ + +  L++ L+ +   +   N++F+       GI  TTK          + 
Sbjct: 550 IEISQQRMETSVEGLLEFLKFV---ELKMNNRFEELVQKMNGIMETTKRHEFMGARAFEE 609

Query: 609 DVEEKEENDEKDDQDDHELE---KNPSHRREDDDGGPTGGKQQQGPTTPRPTTLVQTETR 668
            +++ EE+ E+DD +D  L+   +    +R+DD+      K  +G         +  E++
Sbjct: 610 HLDKVEEDQEEDDVEDLNLDTSNRTVLGKRDDDE-----DKDGKG---------LMDESQ 669

Query: 669 VDGEGTGDGGTKKTGGGEGT-----------KACDDADETINKAILSIDEA----KVIEK 728
            +     DGG  K    E             KA ++ DE IN+ I SIDE+    ++ +K
Sbjct: 670 GESSRGHDGGQSKVAKSETPPKAGDKESSQYKAPEETDEEINRLIRSIDESVIYDEIKKK 729

Query: 729 FNRDRKGKAVMVEGPHTIPRTTVPQLG-----PRWS------------------------ 745
             R R G+  +   P  + R   P  G     P+ +                        
Sbjct: 730 EQRRRTGQYSLNSTPRML-RNVQPAFGKIKHRPQMAKIIGKMPSNDRVSNMAKNTAAARV 789

BLAST of Spg003436 vs. NCBI nr
Match: TYK09852.1 (protein Ycf2-like [Cucumis melo var. makuwa])

HSP 1 Score: 198.0 bits (502), Expect = 2.9e-46
Identity = 174/571 (30.47%), Postives = 268/571 (46.94%), Query Frame = 0

Query: 267 CKPKRTSELYFKIGGKILKFGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDEG 326
           CKPK TS+L F IG ++L+FGLREFALITGL C  +P I+ + ++   R K  YF N + 
Sbjct: 2   CKPKSTSQLQFLIGERVLRFGLREFALITGLRCHEIPNINHEDIKGGGRLKWVYFENLKT 61

Query: 327 VRRKTLNIVFNAMKHGVEADLVKMAQLFVWRAFC-------------------------- 386
           V R+ LN++FN    G + D +KMA+L+   +F                           
Sbjct: 62  VTRQYLNVMFNISTAGTDDDRIKMAKLYFLESFLIPKQECLSVDWDHIIMVDDDEVFDGY 121

Query: 387 ------------YLGK---KRGSVGIGM-GGLVYAILAWAYEVIPALSAPPTNYAKRIRN 446
                       ++ +    +G  GI M GG ++ ILAWAYEVIP LS PP  +A RI N
Sbjct: 122 PWGRVAFELLVDFMNRAVCSKGQTGISMGGGFIFPILAWAYEVIPTLSTPPNFFATRISN 181

Query: 447 TVPRIINWEVEAQPEWRELHVKIFQSSSLEVVPLDPTDTEMQMSYFQPFLQDELASRRLA 506
            VPRIINW  + QP+W++L  K+F S +LEV P+  T  E+ M +F PF + E    + A
Sbjct: 182 EVPRIINWAADTQPKWKDLKQKVFDSPTLEVSPMLATPDEVGMPFFAPFNETEKDILKEA 241

Query: 507 GDEQQVGDDVRIPPNFSV--GAPSMTSQMDVMEKRHQEIIGKLDRVYSMLGALVDTLREI 566
            DE +   +     + S+  G PS TS+++V+ K  ++I     R+ + +  L++ L+ +
Sbjct: 242 EDELRKNKNFDHIASVSLNRGMPS-TSEINVLRKMVEKIEISQQRMETSVEGLLEFLKSV 301

Query: 567 HKLDDPPNSKFKMQGDVGTGIDPTTKDGDVEEKEENDEKDDQDDHELE-KNPSHRREDDD 626
              +   N++F+  G           +  +++ EE+ E+DD +D  L+  N +   + DD
Sbjct: 302 ---ELKMNNRFEELG-------ARAFEEHLDKVEEDQEEDDVEDLNLDTSNRTVLGKRDD 361

Query: 627 GGPTGGKQQQGPTTPRPTTLVQTETRVDGEGTGDGGTKKTGGGEGT-----------KAC 686
                GK+            +  E++ +     DGG  K    E             KA 
Sbjct: 362 DEDKDGKR------------LMDESQGESSRGHDGGQSKVAKSETPPRAGDKESSQYKAP 421

Query: 687 DDADETINKAILSIDEA----KVIEKFNRDRKGKAVMVEGPHTIPRTTVPQLG-----PR 745
           ++ DE IN+ I SIDE+    ++ +K  R R G+  +   P  + R   P  G     P+
Sbjct: 422 EETDEEINRLIRSIDESVIYDEIKKKEQRRRTGQYSLNSTPRML-RNVQPAFGKIKHRPQ 481

BLAST of Spg003436 vs. NCBI nr
Match: XP_038883716.1 (uncharacterized protein LOC120074618 isoform X2 [Benincasa hispida])

HSP 1 Score: 195.7 bits (496), Expect = 1.5e-45
Identity = 147/488 (30.12%), Postives = 227/488 (46.52%), Query Frame = 0

Query: 117 DKASPVTPEIALETSEDTAKNDT------EDTESNSVTNDNSSSDDVGEEREKKKTTIAK 176
           +K SP T E   +  +  ++  T       + E++ +  D  S  +VG ++ K  +  +K
Sbjct: 471 EKRSPTTVEATRKKKKRKSRETTSFEVQEHNEETSEIDTDQDSRRNVGTKQTKNISGQSK 530

Query: 177 KEPPKKQKGGKKGKK----LKTMVEGDTVRVDDDY--LMSPSKISKALKINLCCRTEIMD 236
               K++K  K+GKK           D V V  +Y  L+  S  S   +INL  + +++ 
Sbjct: 531 DGGGKRKKRAKRGKKSGISSTPSEHDDKVDVYKEYPLLLPRSSWSTTQRINLYSKLDVIS 590

Query: 237 TINTILGDRCRDAFRNTCFGHLLDFSFKKTSSQLLLHLIQHQCKPKRTSELYFKIGGKIL 296
            I   L +R    F+ +CFG  LD    K SSQL  HL++ QC     +EL+F + G+I 
Sbjct: 591 VIKNTLNERQLKKFKKSCFGDFLDLKITKFSSQLFYHLVRRQCCSTNRNELWFNLEGRIH 650

Query: 297 KFGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDEGVRRKTLNIVFNAMKHGVE 356
           KFG++EF+LITGLNCG LP+ID  ++Q   +F   YF  ++ ++R  L+ VF  M  G  
Sbjct: 651 KFGMKEFSLITGLNCGELPEIDMSKIQ-KGKFNKRYFGGEKTIKRTKLHEVFTEMDKGRN 710

Query: 357 ADLVKMAQLFVWRAFCYLGKK--------------------------------------- 416
            D+VKMA+L++   F  LGK+                                       
Sbjct: 711 KDVVKMAKLYILEMF-ILGKQIRTGINHEYTLLVDDKEQFDRYPWGRISYEITIDFVKKA 770

Query: 417 ---RGSVGIGMGGLVYAILAWAYEVIPALSAPPTNYAKRIRNTVPRIINWEVEAQPEWRE 476
                +  IG+GG  +A+L WAYE IP L       A R+    PR+ NW  +  PEW++
Sbjct: 771 IKSNDASAIGIGGCAFALLVWAYETIPLLGLNSNFVATRVSFGTPRMNNWAADVHPEWKD 830

Query: 477 LHVKIFQSSSLEVVPLDPTDTEMQMSYFQPFLQDELASRRLAGD-EQQVGDDVRIPPNFS 536
           L  K+FQS S +V PL  T TEM+M Y  PF   + +  ++    +Q+   D R   N  
Sbjct: 831 LSEKVFQSDSFDVQPLIATKTEMEMPYMIPFGGVKPSEEKIKSQLDQEHNSDARTSYNKG 890

Query: 537 V----GAPSMTSQMDVMEKRHQEIIGKLDRVYSMLGALVDTLREIHKLDDPPNSKFKMQG 546
                G PS+ +     +     +  K+  +  +LG+LV      H +D+  N   K+  
Sbjct: 891 YCNWKGPPSVPN-----DGVGNFLFTKIVNIEEILGSLV------HDIDNLKNLFSKLCE 945

BLAST of Spg003436 vs. NCBI nr
Match: XP_038883719.1 (uncharacterized protein LOC120074618 isoform X5 [Benincasa hispida])

HSP 1 Score: 195.3 bits (495), Expect = 1.9e-45
Identity = 148/487 (30.39%), Postives = 228/487 (46.82%), Query Frame = 0

Query: 117 DKASPVTPEIALETSEDTAKNDT------EDTESNSVTNDNSSSDDVGEEREKKKTTIAK 176
           +K SP T E   +  +  ++  T       + E++ +  D  S  +VG ++ K  +  +K
Sbjct: 471 EKRSPTTVEATRKKKKRKSRETTSFEVQEHNEETSEIDTDQDSRRNVGTKQTKNISGQSK 530

Query: 177 KEPPKKQKGGKKGKK---LKTMVEGDTVRVDDDY--LMSPSKISKALKINLCCRTEIMDT 236
               K++K  K+GKK     T  E D    D +Y  L+  S  S   +INL  + +++  
Sbjct: 531 DGGGKRKKRAKRGKKSGISSTPSEHD----DKEYPLLLPRSSWSTTQRINLYSKLDVISV 590

Query: 237 INTILGDRCRDAFRNTCFGHLLDFSFKKTSSQLLLHLIQHQCKPKRTSELYFKIGGKILK 296
           I   L +R    F+ +CFG  LD    K SSQL  HL++ QC     +EL+F + G+I K
Sbjct: 591 IKNTLNERQLKKFKKSCFGDFLDLKITKFSSQLFYHLVRRQCCSTNRNELWFNLEGRIHK 650

Query: 297 FGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDEGVRRKTLNIVFNAMKHGVEA 356
           FG++EF+LITGLNCG LP+ID  ++Q   +F   YF  ++ ++R  L+ VF  M  G   
Sbjct: 651 FGMKEFSLITGLNCGELPEIDMSKIQ-KGKFNKRYFGGEKTIKRTKLHEVFTEMDKGRNK 710

Query: 357 DLVKMAQLFVWRAFCYLGKK---------------------------------------- 416
           D+VKMA+L++   F  LGK+                                        
Sbjct: 711 DVVKMAKLYILEMF-ILGKQIRTGINHEYTLLVDDKEQFDRYPWGRISYEITIDFVKKAI 770

Query: 417 --RGSVGIGMGGLVYAILAWAYEVIPALSAPPTNYAKRIRNTVPRIINWEVEAQPEWREL 476
               +  IG+GG  +A+L WAYE IP L       A R+    PR+ NW  +  PEW++L
Sbjct: 771 KSNDASAIGIGGCAFALLVWAYETIPLLGLNSNFVATRVSFGTPRMNNWAADVHPEWKDL 830

Query: 477 HVKIFQSSSLEVVPLDPTDTEMQMSYFQPFLQDELASRRLAGD-EQQVGDDVRIPPNFSV 536
             K+FQS S +V PL  T TEM+M Y  PF   + +  ++    +Q+   D R   N   
Sbjct: 831 SEKVFQSDSFDVQPLIATKTEMEMPYMIPFGGVKPSEEKIKSQLDQEHNSDARTSYNKGY 890

Query: 537 ----GAPSMTSQMDVMEKRHQEIIGKLDRVYSMLGALVDTLREIHKLDDPPNSKFKMQGD 546
               G PS+ +     +     +  K+  +  +LG+LV      H +D+  N   K+  +
Sbjct: 891 CNWKGPPSVPN-----DGVGNFLFTKIVNIEEILGSLV------HDIDNLKNLFSKLCEN 940

BLAST of Spg003436 vs. NCBI nr
Match: XP_031743195.1 (uncharacterized protein LOC101221625 isoform X8 [Cucumis sativus])

HSP 1 Score: 194.9 bits (494), Expect = 2.5e-45
Identity = 130/385 (33.77%), Postives = 192/385 (49.87%), Query Frame = 0

Query: 117 DKASPVTPEIALETSEDTAKN---DTEDTESNSVTNDNSSSDDVGEEREKKKTTIAKKEP 176
           +K SP + E + +  +  + +     +  E++ +  D  S   V   + KK    +K E 
Sbjct: 613 EKQSPASVEPSRKKKKRKSASFEVQEQKEETSEIDTDQDSRRGVETRQRKKIAEQSKGED 672

Query: 177 PKKQKGGKKGKKLKTMVEGDTVRVDDD---YLMSPSKISKALKINLCCRTEIMDTINTIL 236
            K++K GK+GK  K+ + G +   DD+    L+  S  +   +INL  + +++  I   L
Sbjct: 673 GKRKKRGKRGK--KSGISGTSSEHDDEEYPLLLPRSSWATTQRINLYSKLDVISIIKNTL 732

Query: 237 GDRCRDAFRNTCFGHLLDFSFKKTSSQLLLHLIQHQCKPKRTSELYFKIGGKILKFGLRE 296
            +R    F+ +CFG+ LD    K SSQL  HLI+ QC  K  +EL+F + G+I KFG+++
Sbjct: 733 NERQLKKFKKSCFGNFLDLKISKFSSQLFYHLIRRQCCSKNRNELWFNLEGRIHKFGMKD 792

Query: 297 FALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDEGVRRKTLNIVFNAMKHGVEADLVKM 356
           FALITGLNCG LP ID  ++Q   +F   YF  ++ +RR  L+ VF  M  G   D+VKM
Sbjct: 793 FALITGLNCGELPAIDMSKIQ-KGKFNKRYFGGEKTIRRAKLHKVFTEMDKGRNKDVVKM 852

Query: 357 AQLFVWRAFCYLGKK------------------------------------------RGS 416
           A+L++   F  LGK+                                            +
Sbjct: 853 AKLYILEMF-ILGKQIRTGINHEYTLLIDDKKQFDSYPWGRISYEITVDFVKKSIKSNDA 912

Query: 417 VGIGMGGLVYAILAWAYEVIPALSAPPTNYAKRIRNTVPRIINWEVEAQPEWRELHVKIF 454
             IG+GG  YA+L WAYE IP L+      A RI    PR+ NW     PEW++L  K+F
Sbjct: 913 SAIGVGGFPYALLVWAYETIPLLALNSNFLAMRISFGTPRMNNWAAGVHPEWKDLSEKVF 972

BLAST of Spg003436 vs. ExPASy TrEMBL
Match: A0A5A7U047 (Protein Ycf2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold115G00640 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.4e-64
Identity = 258/845 (30.53%), Postives = 389/845 (46.04%), Query Frame = 0

Query: 9   TRASDRLKAAGVTPGRKPRKQTSPITLGSEQDSGDAMSTSVSVAKGSGEKTKGVKRDRGD 68
           TR SDRL+AAG+T  RK                  ++ T +     S E+   ++    +
Sbjct: 10  TRTSDRLRAAGITGSRK------------------SLPTGIQNLSSSSEER--IEDIMAE 69

Query: 69  GGSGKKVTP-TKKTKVHKRTKKTNDEIEKKP--TGARSNKKKTRAKQTNDTDKASPVTPE 128
           G  GK+ +P T K +V   TKK    ++KK       S K+  R K      K   V  +
Sbjct: 70  GSGGKRESPRTSKKRVRTETKKEEATVQKKQRIKSLVSRKRVYRGK----GKKQKEVEGK 129

Query: 129 IAL-ETSEDTAKNDTEDTESNSVTNDNSSSDDVGEEREK-KKTTIAKKEPPKKQKGGKKG 188
           IA+ E S  +      DT   S            + REK KK    +KE   ++   +KG
Sbjct: 130 IAVGEDSNSSTSEILPDTRKRS------------KAREKGKKVKAVRKEEKAREDRRRKG 189

Query: 189 KKLKTMVEGDTVRVDDDYLMSPSKISKALKINLCCRTEIMDTINTILGDRCRDAFRNTCF 248
           K      + +    D  YLM   + ++ LKINL  ++ +++ I   LGDR  + FR   F
Sbjct: 190 KAPMNPEKSE----DSVYLMCSERRNEPLKINLHIKSTVIEKIKENLGDRLINRFREGIF 249

Query: 249 GHLLDFSFKKTSSQLLLHLIQHQCKPKRTSELYFKIGGKILKFGLREFALITGLNCGPLP 308
           GH L+ S    SSQLLLHLIQ  CKPK TS+L F IGG++L+FGLREFALITGL C  +P
Sbjct: 250 GHFLNLSITNQSSQLLLHLIQRMCKPKSTSQLQFLIGGRVLRFGLREFALITGLRCHEIP 309

Query: 309 QIDTDRLQDSSRFKDEYFANDEGVRRKTLNIVFNAMKHGVEADLVKMAQLFVWRAFC--- 368
            I+ + +    R K  YF N + V R+ LN++FN    G + D +KMA+L+   +F    
Sbjct: 310 DINHEDINGGGRLKGVYFENLKTVTRQYLNVMFNISTTGTDDDRIKMAKLYFLESFLIPK 369

Query: 369 -----------------------------------YLGK---KRGSVGIGMGGLVYAILA 428
                                              ++ +    +G  GI MGG ++ ILA
Sbjct: 370 QECPSVDWDHIIMVDDDEVFDGYPWGRVAFELLVDFMNRVVCSKGQTGISMGGFIFPILA 429

Query: 429 WAYEVIPALSAPPTNYAKRIRNTVPRIINWEVEAQPEWRELHVKIFQSSSLEVVPLDPTD 488
           WAYEVIP LS PP  +  RI N VPRIIN   + QP+W++L  K+F S +LEV P+  T 
Sbjct: 430 WAYEVIPTLSTPPNFFVTRISNEVPRIINCATDTQPKWKDLKQKVFDSPTLEVSPMLATP 489

Query: 489 TEMQMSYFQPFLQDELASRRLAGDEQQVGDDVRIPPNFSV--GAPSMTSQMDVMEKRHQE 548
            E+ M +F PF++ E    + A DE +   +     + S+  G PS TS+++V+ K  ++
Sbjct: 490 DEVGMPFFAPFIETEKDILKEAEDELRKNKNFDHIASVSLNRGMPS-TSEINVLRKMVEK 549

Query: 549 IIGKLDRVYSMLGALVDTLREIHKLDDPPNSKFKMQGDVGTGIDPTTK----------DG 608
           I     R+ + +  L++ L+ +   +   N++F+       GI  TTK          + 
Sbjct: 550 IEISQQRMETSVEGLLEFLKFV---ELKMNNRFEELVQKMNGIMETTKRHEFMGARAFEE 609

Query: 609 DVEEKEENDEKDDQDDHELE---KNPSHRREDDDGGPTGGKQQQGPTTPRPTTLVQTETR 668
            +++ EE+ E+DD +D  L+   +    +R+DD+      K  +G         +  E++
Sbjct: 610 HLDKVEEDQEEDDVEDLNLDTSNRTVLGKRDDDE-----DKDGKG---------LMDESQ 669

Query: 669 VDGEGTGDGGTKKTGGGEGT-----------KACDDADETINKAILSIDEA----KVIEK 728
            +     DGG  K    E             KA ++ DE IN+ I SIDE+    ++ +K
Sbjct: 670 GESSRGHDGGQSKVAKSETPPKAGDKESSQYKAPEETDEEINRLIRSIDESVIYDEIKKK 729

Query: 729 FNRDRKGKAVMVEGPHTIPRTTVPQLG-----PRWS------------------------ 745
             R R G+  +   P  + R   P  G     P+ +                        
Sbjct: 730 EQRRRTGQYSLNSTPRML-RNVQPAFGKIKHRPQMAKIIGKMPSNDRVSNMAKNTAAARV 789

BLAST of Spg003436 vs. ExPASy TrEMBL
Match: A0A5D3CEX9 (Protein Ycf2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold39G00200 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.4e-46
Identity = 174/571 (30.47%), Postives = 268/571 (46.94%), Query Frame = 0

Query: 267 CKPKRTSELYFKIGGKILKFGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDEG 326
           CKPK TS+L F IG ++L+FGLREFALITGL C  +P I+ + ++   R K  YF N + 
Sbjct: 2   CKPKSTSQLQFLIGERVLRFGLREFALITGLRCHEIPNINHEDIKGGGRLKWVYFENLKT 61

Query: 327 VRRKTLNIVFNAMKHGVEADLVKMAQLFVWRAFC-------------------------- 386
           V R+ LN++FN    G + D +KMA+L+   +F                           
Sbjct: 62  VTRQYLNVMFNISTAGTDDDRIKMAKLYFLESFLIPKQECLSVDWDHIIMVDDDEVFDGY 121

Query: 387 ------------YLGK---KRGSVGIGM-GGLVYAILAWAYEVIPALSAPPTNYAKRIRN 446
                       ++ +    +G  GI M GG ++ ILAWAYEVIP LS PP  +A RI N
Sbjct: 122 PWGRVAFELLVDFMNRAVCSKGQTGISMGGGFIFPILAWAYEVIPTLSTPPNFFATRISN 181

Query: 447 TVPRIINWEVEAQPEWRELHVKIFQSSSLEVVPLDPTDTEMQMSYFQPFLQDELASRRLA 506
            VPRIINW  + QP+W++L  K+F S +LEV P+  T  E+ M +F PF + E    + A
Sbjct: 182 EVPRIINWAADTQPKWKDLKQKVFDSPTLEVSPMLATPDEVGMPFFAPFNETEKDILKEA 241

Query: 507 GDEQQVGDDVRIPPNFSV--GAPSMTSQMDVMEKRHQEIIGKLDRVYSMLGALVDTLREI 566
            DE +   +     + S+  G PS TS+++V+ K  ++I     R+ + +  L++ L+ +
Sbjct: 242 EDELRKNKNFDHIASVSLNRGMPS-TSEINVLRKMVEKIEISQQRMETSVEGLLEFLKSV 301

Query: 567 HKLDDPPNSKFKMQGDVGTGIDPTTKDGDVEEKEENDEKDDQDDHELE-KNPSHRREDDD 626
              +   N++F+  G           +  +++ EE+ E+DD +D  L+  N +   + DD
Sbjct: 302 ---ELKMNNRFEELG-------ARAFEEHLDKVEEDQEEDDVEDLNLDTSNRTVLGKRDD 361

Query: 627 GGPTGGKQQQGPTTPRPTTLVQTETRVDGEGTGDGGTKKTGGGEGT-----------KAC 686
                GK+            +  E++ +     DGG  K    E             KA 
Sbjct: 362 DEDKDGKR------------LMDESQGESSRGHDGGQSKVAKSETPPRAGDKESSQYKAP 421

Query: 687 DDADETINKAILSIDEA----KVIEKFNRDRKGKAVMVEGPHTIPRTTVPQLG-----PR 745
           ++ DE IN+ I SIDE+    ++ +K  R R G+  +   P  + R   P  G     P+
Sbjct: 422 EETDEEINRLIRSIDESVIYDEIKKKEQRRRTGQYSLNSTPRML-RNVQPAFGKIKHRPQ 481

BLAST of Spg003436 vs. ExPASy TrEMBL
Match: A0A1S3B0L9 (uncharacterized protein LOC103484737 isoform X5 OS=Cucumis melo OX=3656 GN=LOC103484737 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 7.8e-45
Identity = 135/410 (32.93%), Postives = 196/410 (47.80%), Query Frame = 0

Query: 95  EKKPTGARSNKKKTRAKQTNDTDKASPVTPEIALETSEDTAKNDTEDTESNSVTNDNSSS 154
           ++ P   ++ +KK + K  +              E +E+T++ DT+      V       
Sbjct: 444 KQSPASVKATRKKKKRKSASSE----------VQEQNEETSEIDTDQDSRRGV------- 503

Query: 155 DDVGEEREKKKTTIAKKEP---PKKQKGGKKGKK----LKTMVEGDTVRVDDDY--LMSP 214
               E R+KKK     K+     K++K GKKG K           D V V  +Y  L+  
Sbjct: 504 ----ETRQKKKIAEQSKKQGGHGKRKKRGKKGNKSGVSSSPSEHDDEVDVYKEYPLLLPR 563

Query: 215 SKISKALKINLCCRTEIMDTINTILGDRCRDAFRNTCFGHLLDFSFKKTSSQLLLHLIQH 274
           S  S   +INL  + +++  I   L +R    F+ +CFG+ LD    K SSQL  HLI+ 
Sbjct: 564 SSWSTTQRINLYSKLDVISIIKNTLNERQLKKFKKSCFGYFLDLKVSKFSSQLFYHLIRR 623

Query: 275 QCKPKRTSELYFKIGGKILKFGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDE 334
           QC  K   EL+F + G+I KFG+++FALITGLNCG LP ID  ++Q   +F   YF  ++
Sbjct: 624 QCCSKNRHELWFNLEGRIHKFGMKDFALITGLNCGELPAIDMSKIQ-KGKFNKRYFGGEK 683

Query: 335 GVRRKTLNIVFNAMKHGVEADLVKMAQLFVWRAFCYLGKK-------------------- 394
            +RR  L+ VF  M  G   D+VKMA+L++   F  LGK+                    
Sbjct: 684 TIRRTKLHEVFTEMDKGRNKDVVKMAKLYILEMF-ILGKQLRTGINHEYTLLIDDKEQFD 743

Query: 395 ----------------------RGSVGIGMGGLVYAILAWAYEVIPALSAPPTNYAKRIR 454
                                   +  IG+GG  +A+  WAYE IP L+     +A RI 
Sbjct: 744 SYPWGRISYEITIDFVKKAIKSNDASAIGVGGFPFALCVWAYETIPLLALNSNFFAMRIS 803

BLAST of Spg003436 vs. ExPASy TrEMBL
Match: A0A1S3B065 (uncharacterized protein LOC103484737 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103484737 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 7.8e-45
Identity = 135/410 (32.93%), Postives = 196/410 (47.80%), Query Frame = 0

Query: 95  EKKPTGARSNKKKTRAKQTNDTDKASPVTPEIALETSEDTAKNDTEDTESNSVTNDNSSS 154
           ++ P   ++ +KK + K  +              E +E+T++ DT+      V       
Sbjct: 460 KQSPASVKATRKKKKRKSASSE----------VQEQNEETSEIDTDQDSRRGV------- 519

Query: 155 DDVGEEREKKKTTIAKKEP---PKKQKGGKKGKK----LKTMVEGDTVRVDDDY--LMSP 214
               E R+KKK     K+     K++K GKKG K           D V V  +Y  L+  
Sbjct: 520 ----ETRQKKKIAEQSKKQGGHGKRKKRGKKGNKSGVSSSPSEHDDEVDVYKEYPLLLPR 579

Query: 215 SKISKALKINLCCRTEIMDTINTILGDRCRDAFRNTCFGHLLDFSFKKTSSQLLLHLIQH 274
           S  S   +INL  + +++  I   L +R    F+ +CFG+ LD    K SSQL  HLI+ 
Sbjct: 580 SSWSTTQRINLYSKLDVISIIKNTLNERQLKKFKKSCFGYFLDLKVSKFSSQLFYHLIRR 639

Query: 275 QCKPKRTSELYFKIGGKILKFGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDE 334
           QC  K   EL+F + G+I KFG+++FALITGLNCG LP ID  ++Q   +F   YF  ++
Sbjct: 640 QCCSKNRHELWFNLEGRIHKFGMKDFALITGLNCGELPAIDMSKIQ-KGKFNKRYFGGEK 699

Query: 335 GVRRKTLNIVFNAMKHGVEADLVKMAQLFVWRAFCYLGKK-------------------- 394
            +RR  L+ VF  M  G   D+VKMA+L++   F  LGK+                    
Sbjct: 700 TIRRTKLHEVFTEMDKGRNKDVVKMAKLYILEMF-ILGKQLRTGINHEYTLLIDDKEQFD 759

Query: 395 ----------------------RGSVGIGMGGLVYAILAWAYEVIPALSAPPTNYAKRIR 454
                                   +  IG+GG  +A+  WAYE IP L+     +A RI 
Sbjct: 760 SYPWGRISYEITIDFVKKAIKSNDASAIGVGGFPFALCVWAYETIPLLALNSNFFAMRIS 819

BLAST of Spg003436 vs. ExPASy TrEMBL
Match: A0A1S4DTS6 (uncharacterized protein LOC103484737 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484737 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 7.8e-45
Identity = 135/410 (32.93%), Postives = 196/410 (47.80%), Query Frame = 0

Query: 95  EKKPTGARSNKKKTRAKQTNDTDKASPVTPEIALETSEDTAKNDTEDTESNSVTNDNSSS 154
           ++ P   ++ +KK + K  +              E +E+T++ DT+      V       
Sbjct: 476 KQSPASVKATRKKKKRKSASSE----------VQEQNEETSEIDTDQDSRRGV------- 535

Query: 155 DDVGEEREKKKTTIAKKEP---PKKQKGGKKGKK----LKTMVEGDTVRVDDDY--LMSP 214
               E R+KKK     K+     K++K GKKG K           D V V  +Y  L+  
Sbjct: 536 ----ETRQKKKIAEQSKKQGGHGKRKKRGKKGNKSGVSSSPSEHDDEVDVYKEYPLLLPR 595

Query: 215 SKISKALKINLCCRTEIMDTINTILGDRCRDAFRNTCFGHLLDFSFKKTSSQLLLHLIQH 274
           S  S   +INL  + +++  I   L +R    F+ +CFG+ LD    K SSQL  HLI+ 
Sbjct: 596 SSWSTTQRINLYSKLDVISIIKNTLNERQLKKFKKSCFGYFLDLKVSKFSSQLFYHLIRR 655

Query: 275 QCKPKRTSELYFKIGGKILKFGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDE 334
           QC  K   EL+F + G+I KFG+++FALITGLNCG LP ID  ++Q   +F   YF  ++
Sbjct: 656 QCCSKNRHELWFNLEGRIHKFGMKDFALITGLNCGELPAIDMSKIQ-KGKFNKRYFGGEK 715

Query: 335 GVRRKTLNIVFNAMKHGVEADLVKMAQLFVWRAFCYLGKK-------------------- 394
            +RR  L+ VF  M  G   D+VKMA+L++   F  LGK+                    
Sbjct: 716 TIRRTKLHEVFTEMDKGRNKDVVKMAKLYILEMF-ILGKQLRTGINHEYTLLIDDKEQFD 775

Query: 395 ----------------------RGSVGIGMGGLVYAILAWAYEVIPALSAPPTNYAKRIR 454
                                   +  IG+GG  +A+  WAYE IP L+     +A RI 
Sbjct: 776 SYPWGRISYEITIDFVKKAIKSNDASAIGVGGFPFALCVWAYETIPLLALNSNFFAMRIS 835

BLAST of Spg003436 vs. TAIR 10
Match: AT1G31150.1 (Domain of unknown function (DUF1985) )

HSP 1 Score: 64.7 bits (156), Expect = 3.6e-10
Identity = 44/154 (28.57%), Postives = 74/154 (48.05%), Query Frame = 0

Query: 213 KINLCCRTEIMDTI-NTILGDRCRDAFRNTCFGHLLDFSFKKTS-SQLLLH-LIQHQCKP 272
           ++N+  R E + TI N + G    +  +++ FG L +F   + S S  L+H L+  Q   
Sbjct: 26  RLNIYSRPEYLGTIANLLKGSEEWERIKSSQFGKLFEFPVARCSHSGKLIHGLLSRQVVT 85

Query: 273 KRTSELYFKIGGKILKFGLREFALITGLNCGPLPQIDTDRLQDSSRFKDEYFANDEGVRR 332
           K+  EL+F  GG  ++F +REF ++TGL CG LP  D  +    S++   +         
Sbjct: 86  KKKHELWFVFGGHPIRFSIREFHIVTGLRCGKLPTEDEVKKHQDSKYLSVW--------- 145

Query: 333 KTLNIVFNAMKHGVEADLVKMAQ---LFVWRAFC 361
              N +F   +     D+++M Q   L  W+  C
Sbjct: 146 ---NRLFGEKRMVTIGDVLEMLQKKKLSSWKKLC 167

BLAST of Spg003436 vs. TAIR 10
Match: AT1G36970.1 (Domain of unknown function (DUF1985) )

HSP 1 Score: 55.8 bits (133), Expect = 1.7e-07
Identity = 44/145 (30.34%), Postives = 70/145 (48.28%), Query Frame = 0

Query: 194 TVRVDDDYLMSPSKISKAL---------KINLCCRTEIMDTINTIL-GDRCRDAFRNTCF 253
           T  V+DD + + +++   L         ++N+  R +I+  I  +L G +  +   ++CF
Sbjct: 2   TGAVEDDIIDNDTRLPPRLFATDRYPDARLNIYSRPDILTVICDVLKGTKEVETILDSCF 61

Query: 254 GHLLDFSFKKTS-SQLLLH-LIQHQCKPKRTSELYFKIGGKILKFGLREFALITGLNCGP 313
           G L      + S S  L+H L+  Q   K+  EL    GG+ L+F L EF  +TGL CG 
Sbjct: 62  GSLFSLRVSECSISCKLVHALLCRQLVTKQKYELKMIFGGQPLRFSLVEFGYLTGLPCGE 121

Query: 314 LP-QIDTDRLQDSSRFKDEYFANDE 326
            P + D D      + K E F +DE
Sbjct: 122 FPKEYDPDFFPKRRKGKFELFDDDE 146