Spg003428 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003428
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLipoxygenase
Locationscaffold4: 44724354 .. 44728803 (-)
RNA-Seq ExpressionSpg003428
SyntenySpg003428
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGGATTTGGAAAGAATATTGTAGAGGGTGCCTTGAACACCACTGGAGATCTTGCTGGCTCTGTCATCAATGCTGGAGGCAACATTATAGATAGAGTTTCCAATCTTGGTGGAAAGAAGATCAAAGGGAAAGTGATTCTTATGAGAAGCAATGTTTTGGATTTCACTGAATTCCATTCCTCACTTCTTGATGGCTTCACTGAGCTCTTGGGTGGTGGTGTTACTCTCCAACTCATCAGTGCCACTCAAACTTGTAACTTCTCTTTCTCTAATCTCTCTATGGTTGTTAATTTACAGTACGAAGACTAAATTTGGATATTTGAAAGTATAGAAACTAAAATAGAAGAGGTTTCAGAGTACGTACATGGACTAAAATGGTAATTTTTAACTTATTGAATTCTCTGAATGTTGTTTGATGATTAAAATTTGCAGCCAACGACTCGCGAGGGAAAGTAGGGAAAAAGGCGTTTTTGGAGAGGTGGCTGACTTCAGTCCCACCACTATTTGCTGGAGAGTCTGTGTTTCAAGTTTACTTTGACTGGGAGGATGCCTTTGGATTTCCAGGAGCTTTCTTCATTAAAAATGGGCATACTAGTGAGTTTTTTCTCAAGTCTCTCACCCTTGAAGATGTTCCTGGCTATGGTAGGATCCATTTTGACTGTAACTCGTGGGTCTATCCTTCTGGAAGATACAAGAAAGATCGTATTTTCTTTGCCAATTATGTAATTTCTTTTCCCTTATTCTGTATTCTCTCTCCCTCTTTCATGAAGATTTATGTTTCTAATGGAATTATTTATATAGACATGTCTTCCAAACGATACACCAAATTCACTTCGTAAGTATAGAGAGGAAGAACTACGGAACCTTAGAGGAGATGGAACAGGAGAGCGAAAGGAATGGGATAGAATCTATGACTACGATGTGTACAACGACCTTGCTGACCCAGATGATGGTGATCATCGTCCCATTCTTGGAGGAAGCCAATACCCTTACCCTCGCAGAGGAAGAACAGGAAGACCACGAGCAAGAAAAGGCAAGATTATTAACTTTTTACCTGATCATGTTCACAATCTTGATTTATTTTCTAAAATCATATTTTGTTCATTCACAGACCACAACTATGAGAGCAGATTGTCACAACTGATGAGTTTAGACATATACGTGCCAAAAGATGAAAATTTTGGACACGTGAAGATGTCGGATTTTCTTGGCCATACATTGAAGGCAGTTTCTATATCTATACAACCAGGACTTCAATCTATCTTTGATTTAACTCCAACTGAATTTGACAAATTCAAGGAAGTTGATGATCTCTATGAACGAGGATTTCCCATTCCATTTAATGCTTTCAAGAGCCTCACTGAGGAGCTCACTCCACCCTTGTTCAAAGCACTTTTGAGGAGTGATGGCGAAAAATTTCTCAAATTTCCCACTCCCCAAGTTGTCAAAGGTAAAAATATGGTTTTTTCCCCCATAAAAAGTGCTTTTAGATTAGGAATTTGAACCCTCAATGTGAAGTTTAAACCAACAAGATTTCACCAACTTGAGATTTTATTTTTTTTAGATGTGATATTGTCAATTAAATTAGGATTACTTTGTTTTTTTTTTTTTTTTTACAATATGTGCTGAAGGGGGATTCGAACCTCAAACCTTGTGGTTATCAGTACAACTTTATGCTAGTTGAGCTATGACAGGATTACTTTGTTAGGAACAATAATTTATAAATGATAATGTAAAACTACTAGTTTATATTGGTTAACAGGTATATATTCTCTTTTTATTAATTATCCTATTAGTGTTGAGTATCCAATAAATGACTAAATTTAATATTTAACCTTTTAAATTTAGTAGCAATTTAATATGGTAGCTAGCCTTGTCTTGTATTATATTGTGATTATGAGCTATTAACCTTCCTTTTCTACCATATTTGTTTTCTTAGAAAAAATGTTATTTTCATACCATATGAATCCTCACCATGCTAAATTAGTATCCACCACCATCAAATTAATATTGAAGTCTACAAATAATTTGAGAAATTTAGAGAACTAATCTTTTAAAATGAAGAATTTAAAATATATATGTTTAATACCCAACAGAGTAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATATTTACTAATACAATTTTATTATCGTCAAATGATTTGTAACAGATAATAAAACTGCATGGAGGACGGATGAAGAATTTGCAAGAGAAATGTTAGCAGGAGTCAATCCTCTCGTAATTCGTCGGCTTGAGGTAATCCTTATTCCTTAACAAACTGAGAGTCAATTTTCTCAAATGGCATTTTCTTTTTCAAGTTCTAATCTCAAGTTGTTGATTAAATTTATTTTTACACAATTTACAGGTTTTTCCACCATTAAGTAAGCTTGACCCAAAAGTTTATGGGAATCAAAAAAGTACCATCACAGAAGAACACATAAAGCATGGTTTGGATGGACTCACAGTTGATGAGGTGATTGAAAGGATTTGAAAATAAATTTTATTTTTTCCTTCTGATATTATCAATGAAAATAATAAAGGTTAAATGATGGAAAATACCCCTAAACTTTTAAAAGTTTCTTCATTTTAGCTTCTAAAGTTTGAAGTTTGTTTCAAAATGATATTTGAAGGCTTTTTTCATTAAATTCATGGATGAAAAATGATGTGTTTGCGTATGTATTGTTGCTTATGTGAATAGAATTTGAAGACATATACCTGGAAGAAACAATAAAAATACATGCTACACATCATTTTTTGTCAATAAATTTAACGGAAAATGTCTTCAAGGGTTGTTTTGAACCAAACTTCAAACTTTGAGGGCCAAAATGAAACTTTGAAAGTTTAGGGGTATAATTGAAACCAACCGCATAGTGTAGATGTATTTTTGATAATTTAACCAATAGTAAATAACGAATTATTGTTGATTGGATTGAATAGGCAATCAAACAAAATAAGCTCTACATACTAGATCACCATGATGCATTGATGCCATACCTTAGAAAAATAAATTCAACATCGACAAAAACATATGCCACAAGAACATTGCTCTTTTTGAAAGACGATGGCACTTTGAAGCCTTTGGTTATTGAGTTGAGCTTGCCACACCCTCAAGGAGACGAACTTGGTGCAATCAGCAAACTATACTTTCCAGCAGAAGTTGGAGTTGAAAGCTCCATTTGGCAACTGGCTAAAGCTTATGTAGCTGTAAACGACGTTGGGTACCATCAACTTATCAGTCACTGGTACAAATCTCCGACATATTAATTATATCATTCTTCTTTTATGAGATTATCAACCCTAACGCTTTGAAATTATAGGTTGCATACTCATGCAGTACTCGAACCTTTTGTTATTGCAACACATCGACAATTGAGCGTGATTCATCCAATTCATAAGTTACTCGTTCCTCATTACAAAGACACGATGTTCATAAACGCAGCTGCAAGACAGGTTCTGATTAATGCCAATGGTCTTCTTGAATCAACCCATTTTACATCGAAGTATTCTATGGAACTGTCGTCTTACATATATAAGGAATGGACCTTCCCCGACCAAGCACTACCGACAAATCTCATCAAGAGGTAAACATTATGAACATGAATAATTTCCTTATGAACAAAATTTTCTTGGTTGATCATGAAAAAATCATACATATTAATATTGATTTCCTTTATACCTGTCCAGAGGAATAGCAGTTGAGGACTCAACTTCCCCACATGGAATTCGATTGCTAATAAGTGATTATCCATTTGCTGTCGATGGACTTGAAATTTGGTCAGCCATCAAAACATGGGTCACAGATTATTGTTCTCTATACTACAAAGATGACCAGGCAATCCAAAATGATTTTGAACTCCAATCTTGGTGGAAGGAGCTAAGAGAGAAAGGTCATGTTGACAAAAAAGATGAACCATGGTGGCCAAAAATGCAAACTTTAGTGGAGCTAATCGAAACGTGCACTATTATCATATGGATTGCTTCAGCTCTTCATGCAGCGGTCAATTTTGGACAATACCCTTTTGGAGGTTATGTTCTCAACCGACCAACTACAAGTCGTAGGTTTATGCCCGAAGTTGGCACTGCTGAGTATAAGGAGCTTGAGTCAAACCCTGAGAAGGCTTTTCTGGGAACCATTAGTTCGGAGCTACAGGCACTTGTTGGTATCTCAATTATTGAAATTTTGTCGAAGCATGCTTCTGATGAGGTCTACCTTGGGCAAAGAGCAACAATTGAGTGGACATCTGATAAATTTGCATTGGAAGCTTTTGAGAAATTTGGAAAAGAACTGTTTGAAGTTGAAAATAGAATTATGCAAAGGAATCAAGATGTCAATTTGAAAAATCGATCTGGACCTGTTAACATGCCATACACCTTACTTGTTCCATCAAGTACGGAAGGACTCACTGGCAGAGGAATTCCCAACAGTATCTCTATCTAA

mRNA sequence

ATGTTTGGATTTGGAAAGAATATTGTAGAGGGTGCCTTGAACACCACTGGAGATCTTGCTGGCTCTGTCATCAATGCTGGAGGCAACATTATAGATAGAGTTTCCAATCTTGGTGGAAAGAAGATCAAAGGGAAAGTGATTCTTATGAGAAGCAATGTTTTGGATTTCACTGAATTCCATTCCTCACTTCTTGATGGCTTCACTGAGCTCTTGGGTGGTGGTGTTACTCTCCAACTCATCAGTGCCACTCAAACTTCCAACGACTCGCGAGGGAAAGTAGGGAAAAAGGCGTTTTTGGAGAGGTGGCTGACTTCAGTCCCACCACTATTTGCTGGAGAGTCTGTGTTTCAAGTTTACTTTGACTGGGAGGATGCCTTTGGATTTCCAGGAGCTTTCTTCATTAAAAATGGGCATACTAGTGAGTTTTTTCTCAAGTCTCTCACCCTTGAAGATGTTCCTGGCTATGGTAGGATCCATTTTGACTGTAACTCGTGGGTCTATCCTTCTGGAAGATACAAGAAAGATCGTATTTTCTTTGCCAATTATACATGTCTTCCAAACGATACACCAAATTCACTTCGTAAGTATAGAGAGGAAGAACTACGGAACCTTAGAGGAGATGGAACAGGAGAGCGAAAGGAATGGGATAGAATCTATGACTACGATGTGTACAACGACCTTGCTGACCCAGATGATGGTGATCATCGTCCCATTCTTGGAGGAAGCCAATACCCTTACCCTCGCAGAGGAAGAACAGGAAGACCACGAGCAAGAAAAGGCAAGATTATTAACTTTTTACCTGATCATGTTCACAATCTTGATTTATTTTCTAAAATCATATTTTGTTCATTCACAGACCACAACTATGAGAGCAGATTGTCACAACTGATGAGTTTAGACATATACGTGCCAAAAGATGAAAATTTTGGACACGTGAAGATGTCGGATTTTCTTGGCCATACATTGAAGGCAGTTTCTATATCTATACAACCAGGACTTCAATCTATCTTTGATTTAACTCCAACTGAATTTGACAAATTCAAGGAAGTTGATGATCTCTATGAACGAGGATTTCCCATTCCATTTAATGCTTTCAAGAGCCTCACTGAGGAGCTCACTCCACCCTTGTTCAAAGCACTTTTGAGGAGTGATGGCGAAAAATTTCTCAAATTTCCCACTCCCCAAGTTGTCAAAGATAATAAAACTGCATGGAGGACGGATGAAGAATTTGCAAGAGAAATGTTAGCAGGAGTCAATCCTCTCGTAATTCGTCGGCTTGAGGTTTTTCCACCATTAAGTAAGCTTGACCCAAAAGTTTATGGGAATCAAAAAAGTACCATCACAGAAGAACACATAAAGCATGGTTTGGATGGACTCACAGTTGATGAGGCAATCAAACAAAATAAGCTCTACATACTAGATCACCATGATGCATTGATGCCATACCTTAGAAAAATAAATTCAACATCGACAAAAACATATGCCACAAGAACATTGCTCTTTTTGAAAGACGATGGCACTTTGAAGCCTTTGGTTATTGAGTTGAGCTTGCCACACCCTCAAGGAGACGAACTTGGTGCAATCAGCAAACTATACTTTCCAGCAGAAGTTGGAGTTGAAAGCTCCATTTGGCAACTGGCTAAAGCTTATGTAGCTGTAAACGACGTTGGGTACCATCAACTTATCAGTCACTGGTTGCATACTCATGCAGTACTCGAACCTTTTGTTATTGCAACACATCGACAATTGAGCGTGATTCATCCAATTCATAAGTTACTCGTTCCTCATTACAAAGACACGATGTTCATAAACGCAGCTGCAAGACAGGTTCTGATTAATGCCAATGGTCTTCTTGAATCAACCCATTTTACATCGAAGTATTCTATGGAACTGTCGTCTTACATATATAAGGAATGGACCTTCCCCGACCAAGCACTACCGACAAATCTCATCAAGAGAGGAATAGCAGTTGAGGACTCAACTTCCCCACATGGAATTCGATTGCTAATAAGTGATTATCCATTTGCTGTCGATGGACTTGAAATTTGGTCAGCCATCAAAACATGGGTCACAGATTATTGTTCTCTATACTACAAAGATGACCAGGCAATCCAAAATGATTTTGAACTCCAATCTTGGTGGAAGGAGCTAAGAGAGAAAGGTCATGTTGACAAAAAAGATGAACCATGGTGGCCAAAAATGCAAACTTTAGTGGAGCTAATCGAAACGTGCACTATTATCATATGGATTGCTTCAGCTCTTCATGCAGCGGTCAATTTTGGACAATACCCTTTTGGAGGTTATGTTCTCAACCGACCAACTACAAGTCGTAGGTTTATGCCCGAAGTTGGCACTGCTGAGTATAAGGAGCTTGAGTCAAACCCTGAGAAGGCTTTTCTGGGAACCATTAGTTCGGAGCTACAGGCACTTGTTGGTATCTCAATTATTGAAATTTTGTCGAAGCATGCTTCTGATGAGGTCTACCTTGGGCAAAGAGCAACAATTGAGTGGACATCTGATAAATTTGCATTGGAAGCTTTTGAGAAATTTGGAAAAGAACTGTTTGAAGTTGAAAATAGAATTATGCAAAGGAATCAAGATGTCAATTTGAAAAATCGATCTGGACCTGTTAACATGCCATACACCTTACTTGTTCCATCAAGTACGGAAGGACTCACTGGCAGAGGAATTCCCAACAGTATCTCTATCTAA

Coding sequence (CDS)

ATGTTTGGATTTGGAAAGAATATTGTAGAGGGTGCCTTGAACACCACTGGAGATCTTGCTGGCTCTGTCATCAATGCTGGAGGCAACATTATAGATAGAGTTTCCAATCTTGGTGGAAAGAAGATCAAAGGGAAAGTGATTCTTATGAGAAGCAATGTTTTGGATTTCACTGAATTCCATTCCTCACTTCTTGATGGCTTCACTGAGCTCTTGGGTGGTGGTGTTACTCTCCAACTCATCAGTGCCACTCAAACTTCCAACGACTCGCGAGGGAAAGTAGGGAAAAAGGCGTTTTTGGAGAGGTGGCTGACTTCAGTCCCACCACTATTTGCTGGAGAGTCTGTGTTTCAAGTTTACTTTGACTGGGAGGATGCCTTTGGATTTCCAGGAGCTTTCTTCATTAAAAATGGGCATACTAGTGAGTTTTTTCTCAAGTCTCTCACCCTTGAAGATGTTCCTGGCTATGGTAGGATCCATTTTGACTGTAACTCGTGGGTCTATCCTTCTGGAAGATACAAGAAAGATCGTATTTTCTTTGCCAATTATACATGTCTTCCAAACGATACACCAAATTCACTTCGTAAGTATAGAGAGGAAGAACTACGGAACCTTAGAGGAGATGGAACAGGAGAGCGAAAGGAATGGGATAGAATCTATGACTACGATGTGTACAACGACCTTGCTGACCCAGATGATGGTGATCATCGTCCCATTCTTGGAGGAAGCCAATACCCTTACCCTCGCAGAGGAAGAACAGGAAGACCACGAGCAAGAAAAGGCAAGATTATTAACTTTTTACCTGATCATGTTCACAATCTTGATTTATTTTCTAAAATCATATTTTGTTCATTCACAGACCACAACTATGAGAGCAGATTGTCACAACTGATGAGTTTAGACATATACGTGCCAAAAGATGAAAATTTTGGACACGTGAAGATGTCGGATTTTCTTGGCCATACATTGAAGGCAGTTTCTATATCTATACAACCAGGACTTCAATCTATCTTTGATTTAACTCCAACTGAATTTGACAAATTCAAGGAAGTTGATGATCTCTATGAACGAGGATTTCCCATTCCATTTAATGCTTTCAAGAGCCTCACTGAGGAGCTCACTCCACCCTTGTTCAAAGCACTTTTGAGGAGTGATGGCGAAAAATTTCTCAAATTTCCCACTCCCCAAGTTGTCAAAGATAATAAAACTGCATGGAGGACGGATGAAGAATTTGCAAGAGAAATGTTAGCAGGAGTCAATCCTCTCGTAATTCGTCGGCTTGAGGTTTTTCCACCATTAAGTAAGCTTGACCCAAAAGTTTATGGGAATCAAAAAAGTACCATCACAGAAGAACACATAAAGCATGGTTTGGATGGACTCACAGTTGATGAGGCAATCAAACAAAATAAGCTCTACATACTAGATCACCATGATGCATTGATGCCATACCTTAGAAAAATAAATTCAACATCGACAAAAACATATGCCACAAGAACATTGCTCTTTTTGAAAGACGATGGCACTTTGAAGCCTTTGGTTATTGAGTTGAGCTTGCCACACCCTCAAGGAGACGAACTTGGTGCAATCAGCAAACTATACTTTCCAGCAGAAGTTGGAGTTGAAAGCTCCATTTGGCAACTGGCTAAAGCTTATGTAGCTGTAAACGACGTTGGGTACCATCAACTTATCAGTCACTGGTTGCATACTCATGCAGTACTCGAACCTTTTGTTATTGCAACACATCGACAATTGAGCGTGATTCATCCAATTCATAAGTTACTCGTTCCTCATTACAAAGACACGATGTTCATAAACGCAGCTGCAAGACAGGTTCTGATTAATGCCAATGGTCTTCTTGAATCAACCCATTTTACATCGAAGTATTCTATGGAACTGTCGTCTTACATATATAAGGAATGGACCTTCCCCGACCAAGCACTACCGACAAATCTCATCAAGAGAGGAATAGCAGTTGAGGACTCAACTTCCCCACATGGAATTCGATTGCTAATAAGTGATTATCCATTTGCTGTCGATGGACTTGAAATTTGGTCAGCCATCAAAACATGGGTCACAGATTATTGTTCTCTATACTACAAAGATGACCAGGCAATCCAAAATGATTTTGAACTCCAATCTTGGTGGAAGGAGCTAAGAGAGAAAGGTCATGTTGACAAAAAAGATGAACCATGGTGGCCAAAAATGCAAACTTTAGTGGAGCTAATCGAAACGTGCACTATTATCATATGGATTGCTTCAGCTCTTCATGCAGCGGTCAATTTTGGACAATACCCTTTTGGAGGTTATGTTCTCAACCGACCAACTACAAGTCGTAGGTTTATGCCCGAAGTTGGCACTGCTGAGTATAAGGAGCTTGAGTCAAACCCTGAGAAGGCTTTTCTGGGAACCATTAGTTCGGAGCTACAGGCACTTGTTGGTATCTCAATTATTGAAATTTTGTCGAAGCATGCTTCTGATGAGGTCTACCTTGGGCAAAGAGCAACAATTGAGTGGACATCTGATAAATTTGCATTGGAAGCTTTTGAGAAATTTGGAAAAGAACTGTTTGAAGTTGAAAATAGAATTATGCAAAGGAATCAAGATGTCAATTTGAAAAATCGATCTGGACCTGTTAACATGCCATACACCTTACTTGTTCCATCAAGTACGGAAGGACTCACTGGCAGAGGAATTCCCAACAGTATCTCTATCTAA

Protein sequence

MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILGGSQYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFPIPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPNSISI
Homology
BLAST of Spg003428 vs. NCBI nr
Match: XP_022946090.1 (linoleate 9S-lipoxygenase 6-like [Cucurbita moschata])

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 800/904 (88.50%), Postives = 846/904 (93.58%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTG+LAGSVINAGGNIIDRV++L GKKIKGK+ILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGELAGSVINAGGNIIDRVTHLRGKKIKGKLILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT TSNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHTSNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTL+DVPGYG++HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLQDVPGYGKVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240

Query: 241 GSQ-YPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GSQ YPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQEYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIRRLEVFPP+SKLDPKVYGNQ+STITEEHIK+ L+GLTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRRLEVFPPISKLDPKVYGNQRSTITEEHIKNDLNGLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWLLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVTDYCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTDYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRRF
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRF 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRNQD  LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNQDGKLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. NCBI nr
Match: XP_022946257.1 (linoleate 9S-lipoxygenase 6-like [Cucurbita moschata])

HSP 1 Score: 1642.1 bits (4251), Expect = 0.0e+00
Identity = 800/904 (88.50%), Postives = 845/904 (93.47%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTGDLAGSVINAGGNIIDRV++L GKKIKGK+ILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGDLAGSVINAGGNIIDRVTHLRGKKIKGKLILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT TSNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHTSNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTLEDVPGYG++HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLEDVPGYGKVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRP+LG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPVLG 240

Query: 241 GS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GS QYPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQQYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIR LEVFPP+SKLDPKVYGNQ+STITEEHIK+ L+GLTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRCLEVFPPISKLDPKVYGNQRSTITEEHIKNDLNGLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWLLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVTDYCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTDYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRRF
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRF 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRNQD  LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNQDGKLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. NCBI nr
Match: KAG6579061.1 (Linoleate 9S-lipoxygenase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1640.9 bits (4248), Expect = 0.0e+00
Identity = 798/904 (88.27%), Postives = 845/904 (93.47%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTGDLAGSVINAGGNIIDR+++L GKKIKGK+ILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGDLAGSVINAGGNIIDRLTHLRGKKIKGKLILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT  SNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHNSNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTLEDVPGYG++HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLEDVPGYGKVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240

Query: 241 GSQ-YPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GSQ YPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQEYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIRRLEVFPP+SKLDPK+YGNQ+STITEEHIK+ L+GLTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRRLEVFPPISKLDPKIYGNQRSTITEEHIKNDLNGLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWLLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVTDYCSLYYKDD+AIQND+ELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTDYCSLYYKDDKAIQNDYELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRRF
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRF 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRNQD  LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNQDGKLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. NCBI nr
Match: XP_023545239.1 (linoleate 9S-lipoxygenase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1638.2 bits (4241), Expect = 0.0e+00
Identity = 798/904 (88.27%), Postives = 844/904 (93.36%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKN+VEGALNTTGDLAGSVINAGGNIIDRV++L GKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNLVEGALNTTGDLAGSVINAGGNIIDRVTHLRGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT TSNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHTSNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTLEDVPGYG++HFDCNSWVYPS RYKKDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLEDVPGYGKVHFDCNSWVYPSRRYKKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240

Query: 241 GSQ-YPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GSQ YPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQEYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGE FLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGETFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIRRLEVFPP+SKLDPKVYGNQ+STITEEHIK+ L+GLTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRRLEVFPPVSKLDPKVYGNQRSTITEEHIKNDLNGLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWLLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVTDYCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTDYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT +IWIASALHAAVNFGQYP+GGYVLNRPTTSRRF
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTVIWIASALHAAVNFGQYPYGGYVLNRPTTSRRF 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVG+AEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGSAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRNQD  LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNQDGKLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. NCBI nr
Match: XP_022999018.1 (linoleate 9S-lipoxygenase 6-like [Cucurbita maxima])

HSP 1 Score: 1635.9 bits (4235), Expect = 0.0e+00
Identity = 797/904 (88.16%), Postives = 841/904 (93.03%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTGDLAGSVINAGGNIIDRV++L GKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGDLAGSVINAGGNIIDRVTHLRGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT  SNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHASNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTLEDVPGYG +HFDCNSWVYPS RY KDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLEDVPGYGNVHFDCNSWVYPSRRYNKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRP+LG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPVLG 240

Query: 241 GS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GS QYPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQQYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIRRLE+FPPLSKLDPKVYGNQ+STITEEHIKHGL+ LTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRRLEIFPPLSKLDPKVYGNQRSTITEEHIKHGLNRLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWSLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVT YCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTHYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRR 
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRL 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRN+D +LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNKDGSLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. ExPASy Swiss-Prot
Match: Q41238 (Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE=1 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 564/870 (64.83%), Postives = 682/870 (78.39%), Query Frame = 0

Query: 40  KKIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQT--SNDSRGKVGKKA 99
           KK+KG V++M+ N LDFT+   SL D   E LG  V+ QLIS+ Q+  +N  +GK    A
Sbjct: 14  KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPA 73

Query: 100 FLERWLTSVPPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGR 159
           +LE +L ++ PL AGE+ F V FDW + FG PGAF IKN H +EFFLKSLTLEDVP +G+
Sbjct: 74  YLENFLFTLTPLAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHGK 133

Query: 160 IHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDR 219
           +HF CNSWVYPS RYK DRIFFAN   LP++TP  LRKYRE EL  LRGDGTG+R+ WDR
Sbjct: 134 VHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELLTLRGDGTGKREAWDR 193

Query: 220 IYDYDVYNDLADPDDGDH--RPILGGS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLD 279
           IYDYDVYNDL +PD G+   R  LGGS  YPYPRRGRTGRP  R                
Sbjct: 194 IYDYDVYNDLGNPDQGEQNVRTTLGGSADYPYPRRGRTGRPPTR---------------- 253

Query: 280 LFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQ 339
                     TD   ESR+  ++SLDIYVP+DE FGH+KMSDFL + LK++   I P L 
Sbjct: 254 ----------TDPKSESRIPLILSLDIYVPRDERFGHLKMSDFLTYALKSIVQFILPELH 313

Query: 340 SIFDLTPTEFDKFKEVDDLYERGFPIPFN-AFKSLTEELTPPLFKALLRSDGEKFLKFPT 399
           ++FD TP EFD F++V  LYE G  +P    FK+LT  +   + K LLR+DGE  L+FPT
Sbjct: 314 ALFDGTPNEFDSFEDVLRLYEGGIKLPQGPLFKALTAAIPLEMMKELLRTDGEGILRFPT 373

Query: 400 PQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIK 459
           P V+KD+KTAWRTDEEFAREMLAGVNP++I RL+ FPP SKLDP+ YGNQ STIT EHI+
Sbjct: 374 PLVIKDSKTAWRTDEEFAREMLAGVNPIIISRLQEFPPKSKLDPEAYGNQNSTITAEHIE 433

Query: 460 HGLDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVI 519
             LDGLTVDEA+  NKL+IL+HHD L+PYLR+IN+T+TKTYA+RTLLFL+D+G+LKPL I
Sbjct: 434 DKLDGLTVDEAMNNNKLFILNHHDVLIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAI 493

Query: 520 ELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLE 579
           ELSLPHP GD+ G ISK+Y P++ GVESSIWQLAKAYVAVND G HQLISHWL+THAV+E
Sbjct: 494 ELSLPHPDGDQFGVISKVYTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIE 553

Query: 580 PFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELSS 639
           PFVIAT+RQLSV+HPIHKLL PH++DTM INA ARQ+LINA G+LEST F SK++ME+S+
Sbjct: 554 PFVIATNRQLSVLHPIHKLLYPHFRDTMNINAMARQILINAGGVLESTVFPSKFAMEMSA 613

Query: 640 YIYKEWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDY 699
            +YK+W FPDQALP +L+KRG+AVEDS+SPHG+RLLI DYP+AVDGLEIWSAIK+WVTDY
Sbjct: 614 VVYKDWVFPDQALPADLVKRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDY 673

Query: 700 CSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIASA 759
           CS YY  D+ I  D ELQ+WWKELRE GH DKK+EPWWP+M+T  ELI++CT IIWIASA
Sbjct: 674 CSFYYGSDEEILKDNELQAWWKELREVGHGDKKNEPWWPEMETPQELIDSCTTIIWIASA 733

Query: 760 LHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVGI 819
           LHAAVNFGQYP+ GY+ NRPT SRRFMPE GT EY+EL+ NP+KAFL TI+++LQ L+G+
Sbjct: 734 LHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGV 793

Query: 820 SIIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKNR 879
           S+IEILS+H +DE+YLGQR + EWT DK  L AF+KFGK+L ++E +I+QRN D  L NR
Sbjct: 794 SLIEILSRHTTDEIYLGQRESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILTNR 853

Query: 880 SGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           SGPVN PYTLL P+S  GLTG+GIPNS+SI
Sbjct: 854 SGPVNAPYTLLFPTSEGGLTGKGIPNSVSI 857

BLAST of Spg003428 vs. ExPASy Swiss-Prot
Match: Q43190 (Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2 SV=1)

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 562/870 (64.60%), Postives = 681/870 (78.28%), Query Frame = 0

Query: 40  KKIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQT--SNDSRGKVGKKA 99
           KK+KG V++M+ N LDFT+   SL D   E LG  V+ QLIS+ Q+  +N  +GK    A
Sbjct: 17  KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPA 76

Query: 100 FLERWLTSVPPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGR 159
           +LE +L ++ PL AGE+ F V FDW + FG PGAF IKN H +EFFLKSLTLEDVP +G+
Sbjct: 77  YLENFLFTLTPLAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHGK 136

Query: 160 IHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDR 219
           +HF CNSWVYPS RYK DRIFFAN   LP++TP  LRKYRE EL  LRGDGTG+R+ WDR
Sbjct: 137 VHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELLTLRGDGTGKREAWDR 196

Query: 220 IYDYDVYNDLADPDDGDH--RPILGGS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLD 279
           IYDYDVYNDL +PD G    R  LGGS  YPYPRRGRTGRP  R                
Sbjct: 197 IYDYDVYNDLGNPDQGKENVRTTLGGSADYPYPRRGRTGRPPTR---------------- 256

Query: 280 LFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQ 339
                     TD   ESR+  ++SLDIYVP+DE FGH+KMSDFL + LK++   I P L 
Sbjct: 257 ----------TDPKSESRIPLILSLDIYVPRDERFGHLKMSDFLTYALKSIVQFILPELH 316

Query: 340 SIFDLTPTEFDKFKEVDDLYERGFPIPFN-AFKSLTEELTPPLFKALLRSDGEKFLKFPT 399
           ++FD TP EFD F++V  LYE G  +P    FK+LT+ +   + + LLR+DGE  L+FPT
Sbjct: 317 ALFDGTPNEFDSFEDVLRLYEGGIRLPQGPLFKALTDAIPLEMIRELLRTDGEGILRFPT 376

Query: 400 PQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIK 459
           P V+KD+KTAWRTDEEFAREMLAGVNP++I RL+ FPP SKLDP+ YGNQ STIT EHI+
Sbjct: 377 PLVIKDSKTAWRTDEEFAREMLAGVNPIIISRLQEFPPKSKLDPEAYGNQNSTITAEHIE 436

Query: 460 HGLDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVI 519
             LDGLTVDEA+  NKL+IL+HHD L+PYLR+IN+T+TKTYA+RTLLFL+D+G+LKPL I
Sbjct: 437 DKLDGLTVDEAMNNNKLFILNHHDVLIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAI 496

Query: 520 ELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLE 579
           ELSLPHP GD+ G  SK+Y P++ GVESSIWQLAKAYVAVND G HQLISHWL+THAV+E
Sbjct: 497 ELSLPHPDGDQFGVTSKVYTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIE 556

Query: 580 PFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELSS 639
           PFVIAT+RQLSV+HPIHKLL PH++DTM INA ARQ+LINA G+LEST F SK++ME+S+
Sbjct: 557 PFVIATNRQLSVLHPIHKLLYPHFRDTMNINAMARQILINAGGVLESTVFPSKFAMEMSA 616

Query: 640 YIYKEWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDY 699
            +YK+W FPDQALP +L+KRG+AVEDS+SPHG+RLLI DYP+AVDGLEIWSAIK+WVTDY
Sbjct: 617 VVYKDWVFPDQALPADLVKRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDY 676

Query: 700 CSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIASA 759
           CS YY  D+ I  D ELQ+WWKELRE GH DKK+EPWWP+M+T  ELI++CT IIWIASA
Sbjct: 677 CSFYYGSDEEILKDNELQAWWKELREVGHGDKKNEPWWPEMETPQELIDSCTTIIWIASA 736

Query: 760 LHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVGI 819
           LHAAVNFGQYP+ GY+ NRPT SRRFMPE GT EY+EL+ NP+KAFL TI+++LQ L+G+
Sbjct: 737 LHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGV 796

Query: 820 SIIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKNR 879
           S+IEILS+H +DE+YLGQR + EWT DK  L AF+KFGK+L ++E +I+QRN D  L NR
Sbjct: 797 SLIEILSRHTTDEIYLGQRESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILINR 856

Query: 880 SGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           SGPVN PYTLL P+S  GLTG+GIPNS+SI
Sbjct: 857 SGPVNAPYTLLFPTSEGGLTGKGIPNSVSI 860

BLAST of Spg003428 vs. ExPASy Swiss-Prot
Match: Q43191 (Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2 SV=1)

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 550/871 (63.15%), Postives = 685/871 (78.65%), Query Frame = 0

Query: 39  GKKIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQT--SNDSRGKVGKK 98
           GKK+KG ++LM+ NVLDF + ++SLLDG  E LG  V+LQLIS       N  +GK    
Sbjct: 18  GKKVKGTIVLMKKNVLDFNDVNASLLDGVLEFLGKRVSLQLISVVHADPGNSLQGKRSNP 77

Query: 99  AFLERWLTSVPPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYG 158
           A+LE+WLT+   L AGES F V FDW++  G PGAF I N H +EF+LKSLTLEDVP +G
Sbjct: 78  AYLEKWLTTGTSLVAGESAFDVTFDWDEDIGVPGAFIINNFHFNEFYLKSLTLEDVPNHG 137

Query: 159 RIHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWD 218
            +HF CNSWVYP+ +YK +RIFFAN   LP +TP  LR YRE+EL NLRG+G G+ +EWD
Sbjct: 138 NVHFVCNSWVYPAKKYKSERIFFANQAYLPGETPEPLRNYREKELVNLRGNGNGKLEEWD 197

Query: 219 RIYDYDVYNDLADPDDGDH--RPILGGS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNL 278
           R+YDY +YNDL DP+ G    R ILGGS +YPYPRRGRTGR   +               
Sbjct: 198 RVYDYALYNDLGDPEKGKQYARTILGGSAEYPYPRRGRTGRKPTK--------------- 257

Query: 279 DLFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGL 338
                       D   ESR+  LMSLDIYVP+DE FGH+K+SDFL + LK++   + P  
Sbjct: 258 -----------ADPKSESRIPLLMSLDIYVPRDERFGHIKLSDFLTYALKSIVQFLIPEF 317

Query: 339 QSIFDLTPTEFDKFKEVDDLYERGFPIPFNAF-KSLTEELTPPLFKALLRSDGEKFLKFP 398
           Q++FD TP EFD F++V  LYE G  +P   F K+LT+ +   + K ++R+DGE   KFP
Sbjct: 318 QALFDSTPDEFDSFEDVLKLYEGGIKLPQGPFLKALTDSIPLEILKEIIRTDGEGKFKFP 377

Query: 399 TPQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHI 458
           TPQV++++K++WRTDEEFAREMLAGVNP++I RL+ FPP S+LD +VYGNQ STIT+EHI
Sbjct: 378 TPQVIQEDKSSWRTDEEFAREMLAGVNPVIISRLQEFPPKSQLDSEVYGNQNSTITKEHI 437

Query: 459 KHGLDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTLKPLV 518
           ++ LDGLT+D+AIK N+LYIL+HHD LMPY+R+IN+T+TK YA+RTLLFL+DDGT+KP+ 
Sbjct: 438 ENTLDGLTIDDAIKTNRLYILNHHDILMPYVRRINTTNTKLYASRTLLFLQDDGTMKPVA 497

Query: 519 IELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVL 578
           IELSLPHP GDELGA+SK+Y PA+ GVE SIWQLAKAYVAVND G HQLISHWL+THA +
Sbjct: 498 IELSLPHPDGDELGAVSKVYTPADQGVEGSIWQLAKAYVAVNDSGVHQLISHWLNTHAAI 557

Query: 579 EPFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELS 638
           EPFVIAT+RQLSV+HPIHKLL PH++DTM INA ARQ+LINA G+LE T F +KY+ME+S
Sbjct: 558 EPFVIATNRQLSVLHPIHKLLHPHFRDTMNINALARQILINAGGVLEMTVFPAKYAMEMS 617

Query: 639 SYIYKEWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTD 698
           + +YK W FP+QALP +LIKRG+AVEDS+SPHG+RLLI DYP+AVDGLEIWSAIK+WVT+
Sbjct: 618 AVVYKSWVFPEQALPADLIKRGVAVEDSSSPHGVRLLIQDYPYAVDGLEIWSAIKSWVTE 677

Query: 699 YCSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIAS 758
           YC+ YYK D+ +  D ELQ+WWKELRE+GH DKKDEPWWPKMQT  EL ++CTIIIWIAS
Sbjct: 678 YCNFYYKSDELVLKDNELQAWWKELREEGHGDKKDEPWWPKMQTRQELKDSCTIIIWIAS 737

Query: 759 ALHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVG 818
           ALHAAVNFGQYP+ GY+ NRPT SRRFMPE GT EY+EL++NP+KA+L TI+ +LQ L+G
Sbjct: 738 ALHAAVNFGQYPYAGYLPNRPTLSRRFMPEPGTPEYEELKTNPDKAYLKTITPQLQTLLG 797

Query: 819 ISIIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKN 878
           IS+IEILS+HASDE+YLGQR + EWT D+  + AFE+FGK+L E+E++I+Q N D   KN
Sbjct: 798 ISLIEILSRHASDEIYLGQRDSSEWTKDQEPIAAFERFGKKLSEIEDQIIQMNGDKKWKN 857

Query: 879 RSGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           RSGPVN+PYTLL P+S +GLTG+GIPNS+SI
Sbjct: 858 RSGPVNVPYTLLFPTSEQGLTGKGIPNSVSI 862

BLAST of Spg003428 vs. ExPASy Swiss-Prot
Match: P38415 (Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 556/870 (63.91%), Postives = 679/870 (78.05%), Query Frame = 0

Query: 40  KKIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQT--SNDSRGKVGKKA 99
           KK+KG V++M+ N LDFT+   SL D   E LG  V+ QLIS+ Q+  +N  +GK    A
Sbjct: 17  KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPA 76

Query: 100 FLERWLTSVPPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGR 159
           +LE +L ++ PL AGE+ F V FDW + FG PGAF IKN H +EFFLKSLTLEDVP +G+
Sbjct: 77  YLENFLLTLTPLAAGETAFGVTFDWNEEFGVPGAFVIKNMHINEFFLKSLTLEDVPNHGK 136

Query: 160 IHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDR 219
           +HF CNSWVYPS RYK DRIFFAN   LP++TP  LRKYRE EL  LRGDGTG+R+ WDR
Sbjct: 137 VHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELVTLRGDGTGKREAWDR 196

Query: 220 IYDYDVYNDLADPDDGDH--RPILGGS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLD 279
           IYDYDVYNDL +PD G    R  LGGS  YPYPRRGRTGRP  R                
Sbjct: 197 IYDYDVYNDLGNPDQGKENVRTTLGGSADYPYPRRGRTGRPPTR---------------- 256

Query: 280 LFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQ 339
                     TD   ESR+  ++SLDIYVP+DE FGH+KMSDFL + LK++   I P L 
Sbjct: 257 ----------TDPKSESRIPLILSLDIYVPRDERFGHLKMSDFLTYALKSIVQFILPELH 316

Query: 340 SIFDLTPTEFDKFKEVDDLYERGFPIPFN-AFKSLTEELTPPLFKALLRSDGEKFLKFPT 399
           ++FD TP EFD F++V  LYE G  +P    FK+LT+ +   + + LLR+DGE  L+FPT
Sbjct: 317 ALFDGTPNEFDSFEDVLRLYEGGIKLPQGPLFKALTDAIPLEMIRELLRTDGEGILRFPT 376

Query: 400 PQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIK 459
           P V+KD+KTAWRTDEEFAREMLAGVNP++I RLE FPP SKLDP++YGNQ STIT EHI+
Sbjct: 377 PLVIKDSKTAWRTDEEFAREMLAGVNPVIISRLEEFPPKSKLDPELYGNQNSTITAEHIE 436

Query: 460 HGLDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVI 519
             LDGLT+DEAI  NKL+IL+HHD L+PYLR+IN+T+TKTYA+RTLLFL+D+G+LKPL I
Sbjct: 437 GKLDGLTIDEAINSNKLFILNHHDVLIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAI 496

Query: 520 ELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLE 579
           ELSLPHP GD+ G  SK+Y P++ GVE SIWQLAKAYVAVND G HQLISHWL+THAV+E
Sbjct: 497 ELSLPHPDGDQFGVTSKVYTPSDQGVEGSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIE 556

Query: 580 PFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELSS 639
           PFVIAT+RQLSV+HPIHKLL PH++DTM INA ARQ+LINA G+LEST F SK++ME+S+
Sbjct: 557 PFVIATNRQLSVLHPIHKLLYPHFRDTMNINALARQILINAGGVLESTVFPSKFAMEMSA 616

Query: 640 YIYKEWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDY 699
            +YK+W FPDQALP +L+KRG+AVEDS+SPHG+RLLI DYP+AVDGLEIWSAIK+WVTDY
Sbjct: 617 VVYKDWVFPDQALPADLVKRGVAVEDSSSPHGVRLLIDDYPYAVDGLEIWSAIKSWVTDY 676

Query: 700 CSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIASA 759
           CS YY  ++ I  D ELQ+WWKE+RE GH DKK+EPWW +M+T  ELI++CT IIWIASA
Sbjct: 677 CSFYYGSNEEILKDNELQAWWKEVREVGHGDKKNEPWWAEMETPQELIDSCTTIIWIASA 736

Query: 760 LHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVGI 819
           LHAAVNFGQYP+ GY+ NRPT SR+FMPE GT EY+EL+ NP+KAFL TI+++LQ L+G+
Sbjct: 737 LHAAVNFGQYPYAGYLPNRPTVSRKFMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGV 796

Query: 820 SIIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKNR 879
           S+IEILS+H +DE+YLGQR + EWT DK  L AFE+FG +L ++E +IMQRN +  L NR
Sbjct: 797 SLIEILSRHTTDEIYLGQRESPEWTKDKEPLAAFERFGNKLTDIEKQIMQRNGNNILTNR 856

Query: 880 SGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           +GPVN PYTLL P+S  GLTG+GIPNS+SI
Sbjct: 857 TGPVNAPYTLLFPTSEGGLTGKGIPNSVSI 860

BLAST of Spg003428 vs. ExPASy Swiss-Prot
Match: O24379 (Linoleate 9S-lipoxygenase 2 OS=Solanum tuberosum OX=4113 GN=LOX1.2 PE=1 SV=1)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 558/871 (64.06%), Postives = 676/871 (77.61%), Query Frame = 0

Query: 40  KKIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQ--TSNDSRGKVGKKA 99
           KK+KG V++M  N LDFT+   SL D   E LG  V+ QLIS+ Q   +N  +GK    A
Sbjct: 17  KKLKGTVVMMNKNALDFTDLAGSLTDKAFEFLGQTVSFQLISSVQGDPTNGLQGKHSNPA 76

Query: 100 FLERWLTSVPPLFAG-ESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYG 159
           +LE  L ++ PL AG E+ F V FDW + FG PGAF IKN H +EFFLKSLTLEDVP +G
Sbjct: 77  YLENSLFTLTPLTAGSETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHG 136

Query: 160 RIHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWD 219
           ++HF CNSWVYPS RYK DRIFF N   LP+ TP  LRKYRE EL  LRGDGTG+R+ WD
Sbjct: 137 KVHFVCNSWVYPSFRYKSDRIFFVNQPYLPSKTPELLRKYRENELLTLRGDGTGKREAWD 196

Query: 220 RIYDYDVYNDLADPDDGDH--RPILGGS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNL 279
           RIYDYD+YNDL +PD+G    R  LGGS +YPYPRRGRTGRP  R               
Sbjct: 197 RIYDYDIYNDLGNPDEGKENVRTTLGGSAEYPYPRRGRTGRPPTR--------------- 256

Query: 280 DLFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGL 339
                      TD   ESR+  ++SLDIYVP+DE FGH+KMSDFL + LK++   I P L
Sbjct: 257 -----------TDPKSESRIPLILSLDIYVPRDERFGHLKMSDFLTYALKSIVQFILPEL 316

Query: 340 QSIFDLTPTEFDKFKEVDDLYERGFPIPFN-AFKSLTEELTPPLFKALLRSDGEKFLKFP 399
            ++FD TP EFD F++V  LYE G  +P    FK+LT  +   + + LLR+DGE  L+FP
Sbjct: 317 HALFDGTPNEFDSFEDVLRLYEGGIKLPQGPLFKALTAAIPLEMIRELLRTDGEGILRFP 376

Query: 400 TPQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHI 459
           TP V+KD+KTAWRTDEEFAREMLAGVNP++I RL+ FPP SKLDP+ YGNQ STIT EHI
Sbjct: 377 TPLVIKDSKTAWRTDEEFAREMLAGVNPVIISRLQEFPPKSKLDPEAYGNQNSTITAEHI 436

Query: 460 KHGLDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTLKPLV 519
           +  LDGLTVDEA+  NKL+IL+HHD L+PYLR+IN+T+TKTYA+RTLLFL+D+G+LKPL 
Sbjct: 437 EDKLDGLTVDEAMNNNKLFILNHHDVLIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLA 496

Query: 520 IELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVL 579
           IELSLPHP GD+ G  SK+Y P++ GVESSIWQLAKAYVAVND G HQLISHWL+THAV+
Sbjct: 497 IELSLPHPDGDQFGVTSKVYTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVI 556

Query: 580 EPFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELS 639
           EPFVIAT+RQLSV+HPIHKLL PH++DTM INA ARQ+LINA G+LEST F SK++ME+S
Sbjct: 557 EPFVIATNRQLSVLHPIHKLLYPHFRDTMNINAMARQILINAGGVLESTVFQSKFAMEMS 616

Query: 640 SYIYKEWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTD 699
           + +YK+W FPDQALP +L+KRG+AVEDS+SPHG+RLLI DYP+AVDGLEIWSAIK+WV+D
Sbjct: 617 AVVYKDWVFPDQALPADLVKRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVSD 676

Query: 700 YCSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIAS 759
           YCS YY  D+ I  D ELQ+WWKELRE GH DKK+EPWWP+M+   ELI++CT IIWIAS
Sbjct: 677 YCSFYYGSDEEILKDNELQAWWKELREVGHGDKKNEPWWPEMERPQELIDSCTTIIWIAS 736

Query: 760 ALHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVG 819
           ALHAAVNFGQYP+ GY+ NRPT SRRFMPE GT EY+EL+ NP+KAFL TI+++LQ L+G
Sbjct: 737 ALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLG 796

Query: 820 ISIIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKN 879
           +S+IEILS+H +DE+YLGQR + EWT DK  L AF+KFGK+L ++E +I+QRN D  L N
Sbjct: 797 VSLIEILSRHTTDEIYLGQRESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILTN 856

Query: 880 RSGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           RSGPVN PYTLL P+S  GLTG+GIPNS+SI
Sbjct: 857 RSGPVNAPYTLLFPTSEGGLTGKGIPNSVSI 861

BLAST of Spg003428 vs. ExPASy TrEMBL
Match: A0A6J1G2U0 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111450285 PE=3 SV=1)

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 800/904 (88.50%), Postives = 846/904 (93.58%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTG+LAGSVINAGGNIIDRV++L GKKIKGK+ILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGELAGSVINAGGNIIDRVTHLRGKKIKGKLILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT TSNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHTSNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTL+DVPGYG++HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLQDVPGYGKVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240

Query: 241 GSQ-YPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GSQ YPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQEYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIRRLEVFPP+SKLDPKVYGNQ+STITEEHIK+ L+GLTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRRLEVFPPISKLDPKVYGNQRSTITEEHIKNDLNGLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWLLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVTDYCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTDYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRRF
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRF 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRNQD  LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNQDGKLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. ExPASy TrEMBL
Match: A0A6J1G386 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111450394 PE=3 SV=1)

HSP 1 Score: 1642.1 bits (4251), Expect = 0.0e+00
Identity = 800/904 (88.50%), Postives = 845/904 (93.47%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTGDLAGSVINAGGNIIDRV++L GKKIKGK+ILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGDLAGSVINAGGNIIDRVTHLRGKKIKGKLILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT TSNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHTSNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTLEDVPGYG++HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLEDVPGYGKVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRP+LG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPVLG 240

Query: 241 GS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GS QYPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQQYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIR LEVFPP+SKLDPKVYGNQ+STITEEHIK+ L+GLTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRCLEVFPPISKLDPKVYGNQRSTITEEHIKNDLNGLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWLLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVTDYCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTDYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRRF
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRF 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRNQD  LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNQDGKLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. ExPASy TrEMBL
Match: A0A6J1KIF1 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493534 PE=3 SV=1)

HSP 1 Score: 1635.9 bits (4235), Expect = 0.0e+00
Identity = 797/904 (88.16%), Postives = 841/904 (93.03%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTGDLAGSVINAGGNIIDRV++L GKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGDLAGSVINAGGNIIDRVTHLRGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT  SNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHASNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTLEDVPGYG +HFDCNSWVYPS RY KDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLEDVPGYGNVHFDCNSWVYPSRRYNKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRP+LG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPVLG 240

Query: 241 GS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GS QYPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQQYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFKALLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKALLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIRRLE+FPPLSKLDPKVYGNQ+STITEEHIKHGL+ LTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRRLEIFPPLSKLDPKVYGNQRSTITEEHIKHGLNRLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWSLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVT YCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTHYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRR 
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRL 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRN+D +LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNKDGSLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. ExPASy TrEMBL
Match: A0A6J1KFW5 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493535 PE=3 SV=1)

HSP 1 Score: 1634.8 bits (4232), Expect = 0.0e+00
Identity = 796/904 (88.05%), Postives = 841/904 (93.03%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNIVEGALNTTGDLAGSVINAGGNIIDRV++L GKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIVEGALNTTGDLAGSVINAGGNIIDRVTHLRGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV+LQLISAT  SNDSRGKVG KAFLERWLTS+PPLF GESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSLQLISATHASNDSRGKVGSKAFLERWLTSLPPLFTGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
           +WED FGFPGAF+I+NGHTSEFFLKSLTLEDVPGYG +HFDCNSWVYPS RY KDRIFFA
Sbjct: 121 NWEDDFGFPGAFYIRNGHTSEFFLKSLTLEDVPGYGNVHFDCNSWVYPSRRYNKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+TCLPNDTP  LRKYREEEL NLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRP+LG
Sbjct: 181 NHTCLPNDTPAPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPVLG 240

Query: 241 GS-QYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           GS QYPYPRRGRTGRPRARK                          DHNYESRLSQ+MSL
Sbjct: 241 GSQQYPYPRRGRTGRPRARK--------------------------DHNYESRLSQVMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLGHTLKAVSISI+PGLQSIFD+TPTEFD FKEVDDL++ GFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGHTLKAVSISIKPGLQSIFDVTPTEFDNFKEVDDLFQGGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           +PFNAFK+LTE+LTPPLFK+LLR+DGEKFLKFPTPQVVKDNK+AW TDEEFAREMLAGVN
Sbjct: 361 VPFNAFKTLTEDLTPPLFKSLLRNDGEKFLKFPTPQVVKDNKSAWSTDEEFAREMLAGVN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PLVIRRLE+FPPLSKLDPKVYGNQ+STITEEHIKHGL+ LTVDEAIKQNKLYILDHHDAL
Sbjct: 421 PLVIRRLEIFPPLSKLDPKVYGNQRSTITEEHIKHGLNRLTVDEAIKQNKLYILDHHDAL 480

Query: 481 MPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVGV 540
           MPYLR+IN+TSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAE GV
Sbjct: 481 MPYLRRINATSTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEKGV 540

Query: 541 ESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKD 600
           E SIW LAKAYV VNDVGYHQLISHWLHTHAVLEPF+IATHRQLSVIHPIHKLLVPHYKD
Sbjct: 541 EGSIWSLAKAYVGVNDVGYHQLISHWLHTHAVLEPFIIATHRQLSVIHPIHKLLVPHYKD 600

Query: 601 TMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVED 660
           TMFINA+ARQVLINANGL+ESTHF SKY+MELSSYIYKEWTFPDQALP NLIKRGIAVED
Sbjct: 601 TMFINASARQVLINANGLIESTHFPSKYAMELSSYIYKEWTFPDQALPNNLIKRGIAVED 660

Query: 661 STSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELRE 720
           S SPHG+RLLI+DYPFAVDGLEIWSAIK+WVT YCSLYYKDD+AIQNDFELQSWW ELRE
Sbjct: 661 SNSPHGLRLLINDYPFAVDGLEIWSAIKSWVTHYCSLYYKDDKAIQNDFELQSWWTELRE 720

Query: 721 KGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRF 780
           KGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGYVLNRPTTSRR 
Sbjct: 721 KGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYVLNRPTTSRRL 780

Query: 781 MPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWTS 840
           MPEVGTAEYKELESNPEKAFL TI+SELQAL+GIS+IEILSKHASDEVYLGQRA+IEWTS
Sbjct: 781 MPEVGTAEYKELESNPEKAFLRTITSELQALIGISVIEILSKHASDEVYLGQRASIEWTS 840

Query: 841 DKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPN 900
           DKFALEAFEKFGKELFEVENRIMQRN+D +LKNRSGPVNMPYTLLVPSSTEGLT +GIPN
Sbjct: 841 DKFALEAFEKFGKELFEVENRIMQRNKDGSLKNRSGPVNMPYTLLVPSSTEGLTAKGIPN 878

Query: 901 SISI 904
           SISI
Sbjct: 901 SISI 878

BLAST of Spg003428 vs. ExPASy TrEMBL
Match: A0A5A7TY93 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00920 PE=3 SV=1)

HSP 1 Score: 1596.3 bits (4132), Expect = 0.0e+00
Identity = 774/905 (85.52%), Postives = 832/905 (91.93%), Query Frame = 0

Query: 1   MFGFGKNIVEGALNTTGDLAGSVINAGGNIIDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60
           MFG GKNI+EGALNTTGDLAGSVINAGGNI+DRVSNLGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1   MFGIGKNIIEGALNTTGDLAGSVINAGGNILDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61  SSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLERWLTSVPPLFAGESVFQVYF 120
           SSLLD FTELLGGGV++QLISAT TSNDSRGKVG KA+LERWLTS+PPLFAGESVFQ+ F
Sbjct: 61  SSLLDNFTELLGGGVSIQLISATHTSNDSRGKVGNKAYLERWLTSIPPLFAGESVFQINF 120

Query: 121 DWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIHFDCNSWVYPSGRYKKDRIFFA 180
            WE+ FGFPGAFFI+NGHTSEFFLKSLTL+DVPGYGR+HFDCNSWVYPSGRYKKDRIFFA
Sbjct: 121 PWEENFGFPGAFFIRNGHTSEFFLKSLTLDDVPGYGRVHFDCNSWVYPSGRYKKDRIFFA 180

Query: 181 NYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIYDYDVYNDLADPDDGDHRPILG 240
           N+  LPN TPN LRKYREEEL NLRGDGTGERKEWDRIYDYDVYND+ADPD GDHRPILG
Sbjct: 181 NHVYLPNQTPNPLRKYREEELWNLRGDGTGERKEWDRIYDYDVYNDIADPDVGDHRPILG 240

Query: 241 G-SQYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFSKIIFCSFTDHNYESRLSQLMSL 300
           G ++YPYPRRGRTGRPR+R+                          DHNYESRLS LMSL
Sbjct: 241 GTTEYPYPRRGRTGRPRSRR--------------------------DHNYESRLSPLMSL 300

Query: 301 DIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIFDLTPTEFDKFKEVDDLYERGFP 360
           DIYVPKDENFGH+KMSDFLG+TLKA+SISI+PGLQSIFD++P EFD FKEVD+L+ERGFP
Sbjct: 301 DIYVPKDENFGHLKMSDFLGYTLKALSISIKPGLQSIFDVSPNEFDNFKEVDNLFERGFP 360

Query: 361 IPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVVKDNKTAWRTDEEFAREMLAGVN 420
           IPFNAFK+LTE+LTPPLFKAL+RSD EKFLKFPTPQVVKDNK  W TDEEFAREMLAG N
Sbjct: 361 IPFNAFKTLTEDLTPPLFKALVRSDDEKFLKFPTPQVVKDNKIGWSTDEEFAREMLAGPN 420

Query: 421 PLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHGLDGLTVDEAIKQNKLYILDHHDAL 480
           PL+IRRLEVFPP SKLDP VYGNQ STITEEHIKHGLDGLTVDEA+ QNKLYI+D HDAL
Sbjct: 421 PLLIRRLEVFPPTSKLDPNVYGNQNSTITEEHIKHGLDGLTVDEAMNQNKLYIVDFHDAL 480

Query: 481 MPYLRKINSTS-TKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDELGAISKLYFPAEVG 540
           MPYL ++N+TS TKTYATRTLLFLKDDGTLKPLVIELSLPHPQGD+LGAISKLYFPAE G
Sbjct: 481 MPYLTRMNATSTTKTYATRTLLFLKDDGTLKPLVIELSLPHPQGDQLGAISKLYFPAENG 540

Query: 541 VESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYK 600
           V+ SIWQLAKAYV VNDVGYHQLISHWLHTHAVLEPFVIATHRQLSV+HPIHKLLVPHYK
Sbjct: 541 VQKSIWQLAKAYVTVNDVGYHQLISHWLHTHAVLEPFVIATHRQLSVLHPIHKLLVPHYK 600

Query: 601 DTMFINAAARQVLINANGLLESTHFTSKYSMELSSYIYKEWTFPDQALPTNLIKRGIAVE 660
           DTMFINA+ARQVLINANGL+ESTH+ SKYSMELSS +YK+WTFPDQALP NLIKRG+AVE
Sbjct: 601 DTMFINASARQVLINANGLIESTHYPSKYSMELSSILYKDWTFPDQALPNNLIKRGVAVE 660

Query: 661 DSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELR 720
           DS++PHG+RLLI+DYPFAVDGL+IWSAIKTWV DYCSLYYKDD A+QNDFELQSWW ELR
Sbjct: 661 DSSAPHGLRLLINDYPFAVDGLDIWSAIKTWVQDYCSLYYKDDNAVQNDFELQSWWNELR 720

Query: 721 EKGHVDKKDEPWWPKMQTLVELIETCTIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRR 780
           EKGH DKK EPWWPKMQTL ELIE+CT IIWIASALHAAVNFGQYP+GGY+LNRPTTSRR
Sbjct: 721 EKGHADKKHEPWWPKMQTLSELIESCTTIIWIASALHAAVNFGQYPYGGYILNRPTTSRR 780

Query: 781 FMPEVGTAEYKELESNPEKAFLGTISSELQALVGISIIEILSKHASDEVYLGQRATIEWT 840
           FMPEVGTAEYKELESNPEKAFL TISSELQALV ISIIEILSKHASDEVYLGQRA+I+WT
Sbjct: 781 FMPEVGTAEYKELESNPEKAFLRTISSELQALVSISIIEILSKHASDEVYLGQRASIDWT 840

Query: 841 SDKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIP 900
           SDK ALEAFEKFGKELFEVENRIM+RN+DVNLKNR+GPVN+PYTLLVPSS EGLT RGIP
Sbjct: 841 SDKIALEAFEKFGKELFEVENRIMERNKDVNLKNRAGPVNLPYTLLVPSSNEGLTARGIP 879

Query: 901 NSISI 904
           NSISI
Sbjct: 901 NSISI 879

BLAST of Spg003428 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 537/869 (61.80%), Postives = 672/869 (77.33%), Query Frame = 0

Query: 40  KKIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISA--TQTSNDSRGKVGKKA 99
           KK+KG V+LM+ NVLDF +F++S LD   E LG  +TL+L+S+  T + N S+GK+GK A
Sbjct: 19  KKVKGTVVLMKKNVLDFNDFNASFLDRLHEFLGNKITLRLVSSDVTDSENGSKGKLGKAA 78

Query: 100 FLERWLTSVPPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGR 159
            LE W+T++  L AGES F+V FD+E  FG+PGAF I+N H SEF LKSLTLEDVPG+GR
Sbjct: 79  HLEDWITTITSLTAGESAFKVTFDYETDFGYPGAFLIRNSHFSEFLLKSLTLEDVPGHGR 138

Query: 160 IHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDR 219
           +H+ CNSW+YP+  Y  DR+FF+N T LP++TP +L KYREEEL +LRG G GE KEWDR
Sbjct: 139 VHYICNSWIYPAKHYTTDRVFFSNKTYLPHETPATLLKYREEELVSLRGTGEGELKEWDR 198

Query: 220 IYDYDVYNDLADPDDGDHRPILGGSQ-YPYPRRGRTGRPRARKGKIINFLPDHVHNLDLF 279
           +YDY  YNDL  P   + RP+LGG+Q YPYPRRGRTGR   ++                 
Sbjct: 199 VYDYAYYNDLGVPPK-NPRPVLGGTQEYPYPRRGRTGRKPTKE----------------- 258

Query: 280 SKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSI 339
                    D   ESRL    SLDIYVP+DE FGH+KMSDFL + LKA++  IQP L+++
Sbjct: 259 ---------DPQTESRLPITSSLDIYVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAV 318

Query: 340 FDLTPTEFDKFKEVDDLYERGFPIPFNA-FKSLTEELTPPLFKALLRSDGEKFLKFPTPQ 399
           FD TP EFD F++V  +YE G  +P  A   S+ + +   + K + R+DG+KFLKFP PQ
Sbjct: 319 FDDTPKEFDSFEDVLKIYEEGIDLPNQALIDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQ 378

Query: 400 VVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKHG 459
           V+K++KTAWRTDEEFAREMLAG+NP+VI+ L+ FPP SKLD + YGNQ STIT+ HI+H 
Sbjct: 379 VIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHN 438

Query: 460 LDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTLKPLVIEL 519
           LDGLTV+EA+++ +L+ILDHHD LMPYL ++N+T+TKTYA+RTLLFLKDDGTLKPLVIEL
Sbjct: 439 LDGLTVEEALEKERLFILDHHDTLMPYLGRVNTTTTKTYASRTLLFLKDDGTLKPLVIEL 498

Query: 520 SLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPF 579
           SLPHP GD+ GA+S++Y P E GV  S+WQLAKA+V VND G HQLISHW+ THA +EPF
Sbjct: 499 SLPHPNGDKFGAVSEVYTPGE-GVYDSLWQLAKAFVGVNDSGNHQLISHWMQTHASIEPF 558

Query: 580 VIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELSSYI 639
           VIAT+RQLSV+HP+ KLL PH++DTM INA ARQ+LIN  G+ E T F SKY+ME+SS+I
Sbjct: 559 VIATNRQLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPSKYAMEMSSFI 618

Query: 640 YK-EWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYC 699
           YK  WTFPDQALP  L KRG+AVED  +PHG+RL I DYP+AVDGLE+W AI++WV DY 
Sbjct: 619 YKNHWTFPDQALPAELKKRGMAVEDPEAPHGLRLRIKDYPYAVDGLEVWYAIESWVRDYI 678

Query: 700 SLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIASAL 759
            L+YK ++ IQ D ELQ+WWKE+RE+GH DKK EPWWPKMQT  EL+E+CTIIIW+ASAL
Sbjct: 679 FLFYKIEEDIQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESCTIIIWVASAL 738

Query: 760 HAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVGIS 819
           HAAVNFGQYP  GY+ NRPT SR++MP+  T E++ELE NP+K FL TI+++LQ L+GIS
Sbjct: 739 HAAVNFGQYPVAGYLPNRPTISRQYMPKENTPEFEELEKNPDKVFLKTITAQLQTLLGIS 798

Query: 820 IIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKNRS 879
           +IEILS H+SDEVYLGQR + EW ++K ALEAFEKFG+++ E+E  I +RN D  LKNR+
Sbjct: 799 LIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDERNDDETLKNRT 858

Query: 880 GPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           G V MPYTLL PSS  G+TGRGIPNS+SI
Sbjct: 859 GLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859

BLAST of Spg003428 vs. TAIR 10
Match: AT3G22400.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 1031.6 bits (2666), Expect = 3.9e-301
Identity = 514/880 (58.41%), Postives = 657/880 (74.66%), Query Frame = 0

Query: 41  KIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQ--TSNDSRGKVGKKAF 100
           KI+G+V++M+ N+LDF +  +SLLD   ELLG  V+L LIS+ Q   +N+ RG++GK A 
Sbjct: 34  KIEGEVVVMKKNLLDFKDVMASLLDRVNELLGRRVSLHLISSHQPDPANEKRGRLGKAAH 93

Query: 101 LERWLTSV-PPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVP---- 160
           LE+W+T +   + A E+ F V FDW+++ G P AF IKN H S+F+LKSLTL   P    
Sbjct: 94  LEKWVTKIKTSVTAEETAFGVTFDWDESMGPPAAFVIKNHHHSQFYLKSLTLRGFPDGEG 153

Query: 161 GYGRIHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRG-DGTGER 220
           G   IHF CNSW+YP+ RY+ DR+FF+N   LP++TP  +++ REEEL+NLRG +  GE 
Sbjct: 154 GATAIHFICNSWIYPNHRYRSDRVFFSNKAYLPSETPELIKELREEELKNLRGNEKGGEF 213

Query: 221 KEWDRIYDYDVYNDLADPDDGDH--RPILGGS-QYPYPRRGRTGRPRARKGKIINFLPDH 280
           KEWDR+YDY  YNDL  PD G    RP+LGGS + PYPRRG+TGR   +           
Sbjct: 214 KEWDRVYDYAYYNDLGAPDKGPDSVRPVLGGSPELPYPRRGKTGRKSTK----------- 273

Query: 281 VHNLDLFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISI 340
                          +D   ESRL+ L++L+IYVP+DE F HVK SDFL + LK+V+  +
Sbjct: 274 ---------------SDPKSESRLA-LLNLNIYVPRDERFSHVKFSDFLAYALKSVTQVL 333

Query: 341 QPGLQSIFDLTPTEFDKFKEVDDLYERGFPIPFNAFKSLTEELTP-PLFKALLRSDGEKF 400
            P + S+ D T  EFD F++V  LY+    +      S   ++ P  +F+ L+R+DGE+F
Sbjct: 334 VPEIASVCDKTINEFDSFEDVFHLYDGSIKLANGHTISKLRDVIPWEMFRELVRNDGERF 393

Query: 401 LKFPTPQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTIT 460
           LK+P P ++K++++AWRTDEEFAREMLAG+NP+VI RL+ FPP S LD   YGNQ S+I 
Sbjct: 394 LKYPLPDILKESRSAWRTDEEFAREMLAGLNPVVISRLQEFPPKSCLDSAKYGNQHSSIR 453

Query: 461 EEHIKHGLDGLTVDEAIKQNKLYILDHHDALMPYLRKINSTSTKTYATRTLLFLKDDGTL 520
            EHI+  ++GL V EA++QNKLYILDHHDALMPYL +INST+TKTYATRTLL L+ DGTL
Sbjct: 454 TEHIESNMNGLNVQEALEQNKLYILDHHDALMPYLTRINSTNTKTYATRTLLLLQADGTL 513

Query: 521 KPLVIELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHT 580
           KPL IELSLPH QG+  G++SK++ PAE GVE S+WQLAKAY AVND GYHQLISHWL T
Sbjct: 514 KPLAIELSLPHAQGESYGSVSKVFTPAEKGVEGSVWQLAKAYAAVNDSGYHQLISHWLQT 573

Query: 581 HAVLEPFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYS 640
           HAV+EPF+IA++RQLSV+HPIHKLL PH++DTM INA AR VLIN++G+LE T F S+Y+
Sbjct: 574 HAVIEPFIIASNRQLSVVHPIHKLLHPHFRDTMNINALARHVLINSDGVLERTVFPSRYA 633

Query: 641 MELSSYIYKEWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKT 700
           ME+SS IYK W F +QALP +L+KRG+AVED  S +G++LLI DYPFAVDGLEIWSAIKT
Sbjct: 634 MEMSSSIYKNWVFTEQALPKDLLKRGVAVEDPNSDNGVKLLIEDYPFAVDGLEIWSAIKT 693

Query: 701 WVTDYCSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIII 760
           WVT+YC+ YY +D+ +Q D E+QSWW ELR KGH DK+ E WWP MQT  +LIETCTIII
Sbjct: 694 WVTEYCTFYYNNDKTVQTDTEIQSWWTELRTKGHGDKRHESWWPSMQTRDDLIETCTIII 753

Query: 761 WIASALHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQ 820
           WIASALHAAVNFGQYP+ G++ NRPT SRRFMPE GT EY ELE + + AFL TI+ +LQ
Sbjct: 754 WIASALHAAVNFGQYPYAGFLPNRPTVSRRFMPEPGTDEYAELEEDADVAFLKTITPQLQ 813

Query: 821 ALVGISIIEILSKHASDEVYLGQRATIEWTSDKFALEAFEKFGKELFEVENRIMQRNQDV 880
            L+GISIIEILS H++DE+YLGQR +  WT+D   LEAF++FGKEL  +EN I++RN D 
Sbjct: 814 TLLGISIIEILSMHSTDEIYLGQRDSPNWTADDEPLEAFKRFGKELELIENNIIRRNNDK 873

Query: 881 NLKNRSGPVNMPYTLLVPSSTE-----GLTGRGIPNSISI 904
             KNR+GPVN+PYTLL P++T+     G+TG+GIPNS+SI
Sbjct: 874 RFKNRTGPVNIPYTLLYPNTTDYTREGGITGKGIPNSVSI 886

BLAST of Spg003428 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 698.4 bits (1801), Expect = 7.8e-201
Identity = 373/870 (42.87%), Postives = 516/870 (59.31%), Query Frame = 0

Query: 41  KIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKKAFLE 100
           K++    +   N  DF E     LD FT+ +G  V L+L+S       +  K  K A L+
Sbjct: 91  KVRAVATVRNKNKEDFKETLVKHLDAFTDKIGRNVVLELMSTQVDPKTNEPKKSKAAVLK 150

Query: 101 RWLTSVPPLFAGESV-FQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGRIH 160
            W  S       E V +   F  + AFG PGA  + N H  EFFL+S+T+E     G +H
Sbjct: 151 DW--SKKSNSKAERVHYTAEFTVDSAFGSPGAITVTNKHQKEFFLESITIEGF-ACGPVH 210

Query: 161 FDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDRIY 220
           F CNSWV     +   RI F N   LP++TP+ LR  RE+EL NLRG+G GERK  DRIY
Sbjct: 211 FPCNSWVQSQKDHPSKRILFTNQPYLPSETPSGLRTLREKELENLRGNGKGERKLSDRIY 270

Query: 221 DYDVYNDLADPDDGDH--RPILGGSQYPYPRRGRTGRPRARKGKIINFLPDHVHNLDLFS 280
           DYDVYND+ +PD      RP LGG ++PYPRR RTGR                       
Sbjct: 271 DYDVYNDIGNPDISRELARPTLGGREFPYPRRCRTGR----------------------- 330

Query: 281 KIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQSIF 340
                S TD +  S       L +YVP+DE F   K + F    LKAV  ++ P L++  
Sbjct: 331 -----SSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLKA-- 390

Query: 341 DLTPTEFDKFKEVDDLYERGFPIPFNAFKSLTEELTPPLFKALLRSDGEKFLKFPTPQVV 400
            +   +F  F E+D LY+ G  +       + ++   P     L+   E  L++ TP++V
Sbjct: 391 SILAEDFANFGEIDSLYKEGLLLKLGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTPKIV 450

Query: 401 KDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYG-NQKSTITEEHIKHGL 460
             +K AW  D+EFAR+ +AG+NP+ I R+  +PP+S LDP++YG    S +TE+HI   L
Sbjct: 451 SKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQL 510

Query: 461 DGLTVDEAIKQNKLYILDHHDALMPYLRKINS-TSTKTYATRTLLFLKDDGTLKPLVIEL 520
           DGLTV +A++ N+L+++D+HD  +P+L +IN+    K YATRT+LFL   GTLKP+ IEL
Sbjct: 511 DGLTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIEL 570

Query: 521 SLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLEPF 580
           SLP  Q        ++  P      + +WQLAKA+V  ND G HQL++HWL THA LEPF
Sbjct: 571 SLP-SQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPF 630

Query: 581 VIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELSSYI 640
           ++A HRQLS +HPI KLL PH + T+ INA ARQ LI+A+G++ES     +Y +E+SS  
Sbjct: 631 ILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIESCFTAGQYGLEISSAA 690

Query: 641 YK-EWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTDYC 700
           YK +W F  + LP +LI+RG+AV D T PHG++LL+ DYP+A DGL +WSAI+TWV  Y 
Sbjct: 691 YKNKWRFDMEGLPADLIRRGMAVPDPTQPHGLKLLVEDYPYANDGLLLWSAIQTWVRTYV 750

Query: 701 SLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIASAL 760
             YY +   IQ D ELQ+W+ E    GH D +D  WWPK+ T+ +L+   T IIW+ASA 
Sbjct: 751 ERYYANSNLIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQ 810

Query: 761 HAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVGIS 820
           HAA+NFGQYP+GGYV NRP   RR +P+    E+     +P+K F  ++ S LQ    ++
Sbjct: 811 HAALNFGQYPYGGYVPNRPPLMRRLIPDESDPEFTSFIEDPQKYFFSSMPSLLQTTKFMA 870

Query: 821 IIEILSKHASDEVYLGQRATIE-WTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLKNR 880
           +++ LS H+ DE Y+G+R     WT D   ++AF  F  E+  +E  I +RN+D + +NR
Sbjct: 871 VVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPSRRNR 926

Query: 881 SGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
            G   +PY L+ PSS  G+T RG+PNS+SI
Sbjct: 931 CGAGVLPYELMAPSSEPGVTCRGVPNSVSI 926

BLAST of Spg003428 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 696.8 bits (1797), Expect = 2.3e-200
Identity = 377/872 (43.23%), Postives = 525/872 (60.21%), Query Frame = 0

Query: 41  KIKGKVILMRSNVLDFTEFHSSLLDGFTELLGGGVTLQLISATQTSNDSRGKVGKK---A 100
           K++  V +   N  D  E     LD F + +G  + L+LIS   T  D + K+ KK   A
Sbjct: 85  KVRAVVTVRNKNKEDLKETLVKHLDAFADKIGRNIVLELIS---TQLDPKTKLPKKSNAA 144

Query: 101 FLERWLTSVPPLFAGESVFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLEDVPGYGR 160
            L+ W +      A    +   F  + AFG PGA  + N H  EFFL+S+T+E     G 
Sbjct: 145 VLKDW-SKKSKTKAERVHYTAEFTVDAAFGSPGAITVMNKHQKEFFLESITIEGF-ALGP 204

Query: 161 IHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGERKEWDR 220
           +HF CNSWV     +   RIFF N   LPN+TP+ LR  RE+EL+NLRGDG+G RK  DR
Sbjct: 205 VHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPSGLRVLREKELKNLRGDGSGVRKLSDR 264

Query: 221 IYDYDVYNDLADPDDGDH--RPILGGSQYPYPRRGRTGRPRARKGKIINFLPDHVHNLDL 280
           IYD+DVYNDL +PD      RP LGG + PYPRR RTGR                     
Sbjct: 265 IYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRRCRTGRQ-------------------- 324

Query: 281 FSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQPGLQS 340
                  + +D + ESR+ +   L +YVP+DE F   K   F    LKAV   + P L++
Sbjct: 325 ------STVSDKDAESRVEK--PLPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKA 384

Query: 341 IFDLTPTEFDKFKEVDDLYERGFPIPFNAFKSLTEEL-TPPLFKALLRSDGEKFLKFPTP 400
              +   +F  F E+D LY+ G  +       + ++   P +    L+   +  LK+ TP
Sbjct: 385 --SIVAEDFADFGEIDRLYKEGLLLKLGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTP 444

Query: 401 QVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEEHIKH 460
           +++  +K AW  D+EFAR+ +AG+NP+ I R++ FPP+S LDPK+YG Q S +T++HI  
Sbjct: 445 KILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIG 504

Query: 461 GLDGLTVDEAIKQNKLYILDHHDALMPYLRKINS-TSTKTYATRTLLFLKDDGTLKPLVI 520
            LDG +V +A+++N+LY+LD+HD  +P+L +IN+    K YATRT+ FL   GTLKP+ I
Sbjct: 505 HLDGFSVQQALEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAI 564

Query: 521 ELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHWLHTHAVLE 580
           ELSLP P G +  +  ++  P      + +WQLAKA+V+ ND G HQL++HWL THA LE
Sbjct: 565 ELSLP-PHGPKHRS-KRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLE 624

Query: 581 PFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTSKYSMELSS 640
           PF++A HRQLS +HPI KLL PH + T+ INA ARQ LI+A+G++E       Y ME+S+
Sbjct: 625 PFILAAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLISADGVIEGGFTAGAYGMEMSA 684

Query: 641 YIYK-EWTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWSAIKTWVTD 700
             YK  W F  + LP +LI+RG+A+ D+T PHG++LLI DYP+A DGL +WSAI+TWV  
Sbjct: 685 AAYKSSWRFDMEGLPADLIRRGMAIPDATQPHGLKLLIEDYPYANDGLLLWSAIQTWVRT 744

Query: 701 YCSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETCTIIIWIAS 760
           Y   YY +   I+ D ELQSW+ E    GH D +D  WWP++ T+ +L+   T +IW+AS
Sbjct: 745 YVERYYPNPNLIKTDSELQSWYSESINVGHADLRDADWWPELSTVDDLVSILTTLIWLAS 804

Query: 761 ALHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTISSELQALVG 820
           A HAA+NFGQYP+GGYV NRP   RR +P+    EY    S+PEK +  ++ S  Q    
Sbjct: 805 AQHAALNFGQYPYGGYVPNRPPLMRRLIPDESDPEYASFISHPEKYYFSSMPSLAQTSKF 864

Query: 821 ISIIEILSKHASDEVYLGQRATIE-WTSDKFALEAFEKFGKELFEVENRIMQRNQDVNLK 880
           +++++ LS H+ DE Y+G+R     WT D   +EAF  F  E+  +E  I +RN D + +
Sbjct: 865 MAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRR 919

Query: 881 NRSGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           NR G   +PY LLVPSS  G+T RG+PNS+SI
Sbjct: 925 NRCGAGVLPYELLVPSSEPGVTCRGVPNSVSI 919

BLAST of Spg003428 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 641.3 bits (1653), Expect = 1.1e-183
Identity = 361/881 (40.98%), Postives = 517/881 (58.68%), Query Frame = 0

Query: 35  SNLGGKKIKGKVILMRSNVLD-FTEFHSSLLDGFTELLGGGVTLQLISATQTSNDSRGKV 94
           S   G K    V+ +R  + +  TE     L+ F + +G G+ +QL+S  +  +   GK 
Sbjct: 74  SKYTGSKTVTAVVKIRKKIKEKLTERFEHQLELFMKAIGQGMLIQLVS--EEIDPETGK- 133

Query: 95  GKKAFLERWLTSVPPLFAGES--VFQVYFDWEDAFGFPGAFFIKNGHTSEFFLKSLTLED 154
           G+K+ LE  +  +P         VF   F     FG PGA  + N  ++E  L  + +ED
Sbjct: 134 GRKS-LESPVMGLPKAVKDPRYLVFTADFTVPINFGKPGAILVTNLLSTEICLSEIIIED 193

Query: 155 VPGYGRIHFDCNSWVYPSGRYKKDRIFFANYTCLPNDTPNSLRKYREEELRNLRGDGTGE 214
                 I F  N+W++      + RI F +  CLP++TP+ +++ RE++L ++RGDG GE
Sbjct: 194 --STDTILFPANTWIHSKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGE 253

Query: 215 RKEWDRIYDYDVYNDLADPDDGDH-RPILGGSQYPYPRRGRTGRPRARKGKIINFLPDHV 274
           RK  +RIYDYDVYNDL DP   +  RP+LG  + PYPRR RTGRP   K           
Sbjct: 254 RKPHERIYDYDVYNDLGDPRKTERVRPVLGVPETPYPRRCRTGRPLVSK----------- 313

Query: 275 HNLDLFSKIIFCSFTDHNYESRLSQLMSLDIYVPKDENFGHVKMSDFLGHTLKAVSISIQ 334
                          D   ESR  +    + YVP+DE F  +K   F     KA+  ++ 
Sbjct: 314 ---------------DPPCESRGKE--KEEFYVPRDEVFEEIKRDTFRAGRFKALFHNLV 373

Query: 335 PGLQSIFDLTPTEFDKFKEVDDLYERGFPIPFNAFKSLTEELTPPLFKALLRSDGEKFLK 394
           P + +        F  F ++D+LY+    +     K          F   + +  E  LK
Sbjct: 374 PSIAAALSNLDIPFTCFSDIDNLYKSNIVLGHTEPKDTGLGGFIGGFMNGILNVTETLLK 433

Query: 395 FPTPQVVKDNKTAWRTDEEFAREMLAGVNPLVIRRLEVFPPLSKLDPKVYGNQKSTITEE 454
           + TP V+K ++ AW  D EF R+ LAGVNP+ I  L+  P  S LDP +YG Q+S +TEE
Sbjct: 434 YDTPAVIKWDRFAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVLTEE 493

Query: 455 HIKHGLD--GLTVDEAIKQNKLYILDHHDALMPYLRKINSTS---TKTYATRTLLFLKDD 514
            I   ++  G T+++A+++ +L+++D+HD L+P++ KINS      KTYA+RT+ F   +
Sbjct: 494 IIAREVEHYGTTIEKALEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKN 553

Query: 515 GTLKPLVIELSLPHPQGDELGAISKLYFPAEVGVESSIWQLAKAYVAVNDVGYHQLISHW 574
           G L+PL IELSLP     E   +   Y          IW+LAKA+V  ND G HQL++HW
Sbjct: 554 GALRPLAIELSLPPTAESENKFV---YTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHW 613

Query: 575 LHTHAVLEPFVIATHRQLSVIHPIHKLLVPHYKDTMFINAAARQVLINANGLLESTHFTS 634
           L THA +EP++IAT+RQLS +HP++KLL PH + T+ INA AR+ LIN  G++ES     
Sbjct: 614 LRTHASMEPYIIATNRQLSTMHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPG 673

Query: 635 KYSMELSSYIYKE-WTFPDQALPTNLIKRGIAVEDSTSPHGIRLLISDYPFAVDGLEIWS 694
           KY+MELSS  YK  W F  + LP +L++RG+A EDS++  G+RL+I DYP+A DGL IW 
Sbjct: 674 KYAMELSSAAYKSMWRFDMEGLPADLVRRGMAEEDSSAECGVRLVIDDYPYAADGLLIWK 733

Query: 695 AIKTWVTDYCSLYYKDDQAIQNDFELQSWWKELREKGHVDKKDEPWWPKMQTLVELIETC 754
           AIK  V  Y   +Y D ++I +D ELQ+WW E++ KGH DKKDEPWWPK+ T  +L +  
Sbjct: 734 AIKDLVESYVKHFYSDSKSITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQIL 793

Query: 755 TIIIWIASALHAAVNFGQYPFGGYVLNRPTTSRRFMPEVGTAEYKELESNPEKAFLGTIS 814
           T +IWIAS  HAA+NFGQYPFGGYV NRPT  R+ +P+    +Y+    NP+ +FLG++ 
Sbjct: 794 TNMIWIASGQHAAINFGQYPFGGYVPNRPTLLRKLIPQETDPDYEMFMRNPQYSFLGSLP 853

Query: 815 SELQALVGISIIEILSKHASDEVYLGQRATIE--WTSDKFALEAFEKFGKELFEVENRIM 874
           ++LQA   +++ E LS H+ DE YL +   ++  W  D+  ++ F KF +EL ++E  I 
Sbjct: 854 TQLQATKVMAVQETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTIN 913

Query: 875 QRNQDVNLKNRSGPVNMPYTLLVPSSTEGLTGRGIPNSISI 904
           +RN+D  LKNR+G    PY LL+P+S  G+TGRGIPNSISI
Sbjct: 914 ERNKDKKLKNRTGAGMPPYELLLPTSPHGVTGRGIPNSISI 917

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022946090.10.0e+0088.50linoleate 9S-lipoxygenase 6-like [Cucurbita moschata][more]
XP_022946257.10.0e+0088.50linoleate 9S-lipoxygenase 6-like [Cucurbita moschata][more]
KAG6579061.10.0e+0088.27Linoleate 9S-lipoxygenase 6, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023545239.10.0e+0088.27linoleate 9S-lipoxygenase 6-like [Cucurbita pepo subsp. pepo][more]
XP_022999018.10.0e+0088.16linoleate 9S-lipoxygenase 6-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q412380.0e+0064.83Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE... [more]
Q431900.0e+0064.60Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2... [more]
Q431910.0e+0063.15Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2... [more]
P384150.0e+0063.91Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1[more]
O243790.0e+0064.06Linoleate 9S-lipoxygenase 2 OS=Solanum tuberosum OX=4113 GN=LOX1.2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G2U00.0e+0088.50Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111450285 PE=3 SV=1[more]
A0A6J1G3860.0e+0088.50Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111450394 PE=3 SV=1[more]
A0A6J1KIF10.0e+0088.16Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493534 PE=3 SV=1[more]
A0A6J1KFW50.0e+0088.05Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111493535 PE=3 SV=1[more]
A0A5A7TY930.0e+0085.52Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00920 P... [more]
Match NameE-valueIdentityDescription
AT1G55020.10.0e+0061.80lipoxygenase 1 [more]
AT3G22400.13.9e-30158.41PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G72520.17.8e-20142.87PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G17420.12.3e-20043.23lipoxygenase 3 [more]
AT1G67560.11.1e-18340.98PLAT/LH2 domain-containing lipoxygenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001246Lipoxygenase, plantPRINTSPR00468PLTLPOXGNASEcoord: 389..405
score: 55.44
coord: 816..830
score: 78.58
coord: 150..168
score: 63.72
coord: 494..518
score: 78.81
coord: 428..447
score: 62.98
coord: 176..197
score: 62.57
IPR013819Lipoxygenase, C-terminalPRINTSPR00087LIPOXYGENASEcoord: 579..599
score: 64.42
coord: 559..576
score: 70.16
coord: 541..558
score: 59.13
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 285..886
e-value: 7.8E-272
score: 903.7
coord: 193..260
e-value: 3.6E-27
score: 95.1
IPR013819Lipoxygenase, C-terminalPROSITEPS51393LIPOXYGENASE_3coord: 183..903
score: 295.586304
IPR001024PLAT/LH2 domainSMARTSM00308LH2_4coord: 40..181
e-value: 4.0E-66
score: 235.7
IPR001024PLAT/LH2 domainPFAMPF01477PLATcoord: 86..179
e-value: 3.6E-16
score: 59.8
IPR001024PLAT/LH2 domainPROSITEPS50095PLATcoord: 57..180
score: 22.094624
NoneNo IPR availableGENE3D3.10.450.60coord: 403..536
e-value: 4.1E-57
score: 194.1
NoneNo IPR availableGENE3D4.10.375.10coord: 185..312
e-value: 1.3E-37
score: 130.1
NoneNo IPR availableGENE3D1.20.245.10coord: 537..902
e-value: 7.7E-160
score: 533.9
NoneNo IPR availableGENE3D2.60.60.20PLAT/LH2 domaincoord: 32..183
e-value: 2.3E-57
score: 195.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..255
NoneNo IPR availablePANTHERPTHR11771:SF126LINOLEATE 9S-LIPOXYGENASE 1coord: 36..259
coord: 285..903
IPR027433Lipoxygenase, domain 3GENE3D4.10.372.10coord: 313..402
e-value: 3.1E-25
score: 90.3
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 36..259
coord: 285..903
IPR020834Lipoxygenase, conserved sitePROSITEPS00081LIPOXYGENASE_2coord: 586..596
IPR020833Lipoxygenase, iron binding sitePROSITEPS00711LIPOXYGENASE_1coord: 559..573
IPR042057Plant lipoxygenase, PLAT/LH2 domainCDDcd01751PLAT_LH2coord: 40..181
e-value: 3.06276E-48
score: 165.557
IPR036392PLAT/LH2 domain superfamilySUPERFAMILY49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 40..183
IPR036226Lipoxigenase, C-terminal domain superfamilySUPERFAMILY48484Lipoxigenasecoord: 185..903

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003428.1Spg003428.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0031408 oxylipin biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding
molecular_function GO:0016491 oxidoreductase activity