Spg003427 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003427
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	nicotianamine synthase-like
Location	scaffold4: 43946117 .. 43946950 (-)
RNA-Seq Expression	Spg003427
Synteny	Spg003427

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTGCCAAGGTCTCCTTTTGGTCCAAAAAGTCTCAGAACTTTACCACAAAATCTCCACTCTCGAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCTCAACTCGTCCTCATCTGCTCGCCTCCGCCGCCGTCTCCGCCGCCCATAGACGTCTCCAAGCTCTGCTCCGCCGTCCAAAATATGAGATCCCATCTCATCAAGCTCTGCGGCGAGGCTGAGGCCCTTCTCGAGCACCATTACTCCACCATTTTAGCCTCCCACCAAAACCCACTTGACCATCTCTCCATTTTCCCTTATTACTCCAACTATCTCAAACTAACCCACCTTGAATTCTCCATTTTGACCCGTCACGCGCCCCACGCGCCGCCGTCCAAGGTGGCATTTGTTGGCTCCGGTGCGCTTCCGTTCTCGTCCATCATCATGGCCTTGAAGTACCTCCCGACGACCGAGTTTCACAACTTCGACGTCGACCCGTCTGCCAATGCAAAAGCGACGGCGTTGGTGGCGGGCGACGCGGACTTGTCGAGGCGGATGACGTTCCACACGACGGATATCATGGAGGTGAATGCGAGTGAGTTGGGGGAGTTTGAGGTGGTGTTTTTGGCGGCGTTGGTGGGGATGGAGAGGGAGGAGAAGGTGAAGGTGATCGAGCATTTGGGGAAGAACATGGCCGGCGGCGCGTTGTTGATGTTGAGGAGCGCGTATGGTGCACGCGCGTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACCGTGTTTCATCCGACTGACGAAGTTATCAATTCGATCGTGTTGGCACGATTATAA

mRNA sequence

ATGTGTTGCCAAGGTCTCCTTTTGGTCCAAAAAGTCTCAGAACTTTACCACAAAATCTCCACTCTCGAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCTCAACTCGTCCTCATCTGCTCGCCTCCGCCGCCGTCTCCGCCGCCCATAGACGTCTCCAAGCTCTGCTCCGCCGTCCAAAATATGAGATCCCATCTCATCAAGCTCTGCGGCGAGGCTGAGGCCCTTCTCGAGCACCATTACTCCACCATTTTAGCCTCCCACCAAAACCCACTTGACCATCTCTCCATTTTCCCTTATTACTCCAACTATCTCAAACTAACCCACCTTGAATTCTCCATTTTGACCCGTCACGCGCCCCACGCGCCGCCGTCCAAGGTGGCATTTGTTGGCTCCGGTGCGCTTCCGTTCTCGTCCATCATCATGGCCTTGAAGTACCTCCCGACGACCGAGTTTCACAACTTCGACGTCGACCCGTCTGCCAATGCAAAAGCGACGGCGTTGGTGGCGGGCGACGCGGACTTGTCGAGGCGGATGACGTTCCACACGACGGATATCATGGAGGTGAATGCGAGTGAGTTGGGGGAGTTTGAGGTGGTGTTTTTGGCGGCGTTGGTGGGGATGGAGAGGGAGGAGAAGGTGAAGGTGATCGAGCATTTGGGGAAGAACATGGCCGGCGGCGCGTTGTTGATGTTGAGGAGCGCGTATGGTGCACGCGCGTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACCGTGTTTCATCCGACTGACGAAGTTATCAATTCGATCGTGTTGGCACGATTATAA

Coding sequence (CDS)

ATGTGTTGCCAAGGTCTCCTTTTGGTCCAAAAAGTCTCAGAACTTTACCACAAAATCTCCACTCTCGAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCTCAACTCGTCCTCATCTGCTCGCCTCCGCCGCCGTCTCCGCCGCCCATAGACGTCTCCAAGCTCTGCTCCGCCGTCCAAAATATGAGATCCCATCTCATCAAGCTCTGCGGCGAGGCTGAGGCCCTTCTCGAGCACCATTACTCCACCATTTTAGCCTCCCACCAAAACCCACTTGACCATCTCTCCATTTTCCCTTATTACTCCAACTATCTCAAACTAACCCACCTTGAATTCTCCATTTTGACCCGTCACGCGCCCCACGCGCCGCCGTCCAAGGTGGCATTTGTTGGCTCCGGTGCGCTTCCGTTCTCGTCCATCATCATGGCCTTGAAGTACCTCCCGACGACCGAGTTTCACAACTTCGACGTCGACCCGTCTGCCAATGCAAAAGCGACGGCGTTGGTGGCGGGCGACGCGGACTTGTCGAGGCGGATGACGTTCCACACGACGGATATCATGGAGGTGAATGCGAGTGAGTTGGGGGAGTTTGAGGTGGTGTTTTTGGCGGCGTTGGTGGGGATGGAGAGGGAGGAGAAGGTGAAGGTGATCGAGCATTTGGGGAAGAACATGGCCGGCGGCGCGTTGTTGATGTTGAGGAGCGCGTATGGTGCACGCGCGTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACCGTGTTTCATCCGACTGACGAAGTTATCAATTCGATCGTGTTGGCACGATTATAA

Protein sequence

MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSAVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSILTRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL

Homology

[Close]

BLAST of Spg003427 vs. NCBI nr
Match: XP_038891237.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 495.4 bits (1274), Expect = 3.3e-136
Identity = 251/277 (90.61%), Postives = 260/277 (93.86%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           MCCQGLLLVQK+SELYHKISTLESLKPSKDVNSLF+QLVLICSPPP SP  IDVS LCSA
Sbjct: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFTQLVLICSPPPSSPSSIDVSTLCSA 60

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSILTRH 120
           VQ MRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLS+FPYYSNYLKLTHLEFSIL  H
Sbjct: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 120

Query: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSRR 180
           APHAPPSKVAFVGSGALPFSSIIMALKYLP TE HNFD+DPSANAKA  LVAGDA+LS+R
Sbjct: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPKTELHNFDIDPSANAKAAMLVAGDAELSKR 180

Query: 181 MTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYGA 240
           M FHTTDIM+V + EL EFEVVFLAALVGMEREEKVKVIEHL K+M GGALLMLRSAYGA
Sbjct: 181 MVFHTTDIMDVASDELREFEVVFLAALVGMEREEKVKVIEHLRKSMGGGALLMLRSAYGA 240

Query: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 277

[Close]

BLAST of Spg003427 vs. NCBI nr
Match: XP_022156409.1 (probable nicotianamine synthase 4 [Momordica charantia])

HSP 1 Score: 463.4 bits (1191), Expect = 1.4e-126
Identity = 242/277 (87.36%), Postives = 253/277 (91.34%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           MCCQ +LLVQKVSELYHKISTL+SLKPSKDVNSLFSQLVL C+PPPPSPPPIDVSKL SA
Sbjct: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSILTRH 120
           VQ MRSHLIKLCGEAEALLEHHYS+ILASH+NPL++LSIFPYYSNYLKL HLEFSIL R 
Sbjct: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILER- 120

Query: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSRR 180
             HA PSKVAFVGSGALPF+SIIMA KYLP TEFHNFDVDPSANAKA ALVAGD DL RR
Sbjct: 121 --HAQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERR 180

Query: 181 MTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYGA 240
           M FHT DIMEV   EL  F+VVFLAALVGMEREEKVKVIEHLGKNM GGALLMLRSAYGA
Sbjct: 181 MVFHTADIMEV-TGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240

Query: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           RAFLYPVVEA DL+GFEILTVFHPTDEVINS+VLARL
Sbjct: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of Spg003427 vs. NCBI nr
Match: KAG7021049.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.1 bits (1185), Expect = 6.9e-126
Identity = 237/282 (84.04%), Postives = 255/282 (90.43%), Query Frame = 0


Query: 1   MCC--QGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICS---PPPPSPPPIDVS 60
           MCC  +GLLLVQKVSELYHKISTL+SL+PSK VNSLFSQLVL C+   PPPPSPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLS+FPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDA 180
           ILTR+   +PPSKVAF+GSGALPFSSIIMALKYLPTTEFHNFDVD SANAKAT LVA D 
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180

Query: 181 DLSRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLR 240
           DLS+RM FHTTDIM+V ++ LGEFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Spg003427 vs. NCBI nr
Match: XP_004144812.1 (nicotianamine synthase 1 [Cucumis sativus])

HSP 1 Score: 461.1 bits (1185), Expect = 6.9e-126
Identity = 239/281 (85.05%), Postives = 254/281 (90.39%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLV-LICSPPPPSPPPIDVSKLCS 60
           MCCQ LLLVQK+SELYHKISTLESLKPSK VNSLFSQLV LIC+   PS   IDVS LCS
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICT---PSSSSIDVSTLCS 60

Query: 61  AVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSILTR 120
            +Q MRSHLIKLCGEAE+LLEHHYSTIL+SHQNPLDHLS+FPYYSNYLKLTHLEFSIL  
Sbjct: 61  TIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNH 120

Query: 121 HAPHA---PPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDAD 180
           HAPH+   PPSKVAFVGSGALPFSSIIMALKY P TEFHNFD+DPSANAKA+ LVAGD +
Sbjct: 121 HAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVE 180

Query: 181 LSRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRS 240
           LSRRM FHTTDIM+V + EL EFEVVFLAALVGMEREEK KVIEHL KNM+GGALLMLRS
Sbjct: 181 LSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           AYGARAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 241 AYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of Spg003427 vs. NCBI nr
Match: XP_022938003.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 460.3 bits (1183), Expect = 1.2e-125
Identity = 238/282 (84.40%), Postives = 255/282 (90.43%), Query Frame = 0


Query: 1   MCC--QGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICS---PPPPSPPPIDVS 60
           MCC  +GLLLVQKVSELYHKISTL+SL+PSK VNSLFSQLVL C+   PPPPSPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLS+FPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDA 180
           ILTR+   + PSKVAF+GSGALPFSSIIMALKYLPTTEFHNFDVD SANAKATALVA D 
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLSRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLR 240
           DLSRRM+FHTTDIM+V +  LGEFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Spg003427 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 6.3e-90
Identity = 166/276 (60.14%), Postives = 219/276 (79.35%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           +C     +V+KV ELY +IS LE+L PSKDVN LF+ LV  C P    P PIDVSKLC  
Sbjct: 2   VCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMP----PNPIDVSKLCQK 61

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSILTRH 120
           +Q +RSHLIKLCG+AE LLE H+S IL+S++NPL HL IFPY+ NY+KL+ LE++ILT++
Sbjct: 62  IQEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKN 121

Query: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSRR 180
             +  P K+AF+GSG LP +S+++A K+L TT FHN+D+D  AN  A+ALVA D D+S R
Sbjct: 122 TTNI-PKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSR 181

Query: 181 MTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYGA 240
           MTFHT D+M+V  + L +++VVFLAALVGM++E+KVKV++HL K M+ GA LMLRSA+GA
Sbjct: 182 MTFHTADVMDVTCA-LKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGA 241

Query: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           RAFLYPV++  DLRGFE+L+V+HPTDEVINS+++AR
Sbjct: 242 RAFLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

[Close]

BLAST of Spg003427 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.6e-88
Identity = 170/277 (61.37%), Postives = 216/277 (77.98%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           M CQ   LVQ + +LY KIS LESLKPS+DVN LF QLV  C PP P+   IDV+K+C  
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN---IDVTKMCDR 60

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSIFPYYSNYLKLTHLEFSILTR 120
           VQ +R +LIK+CG AE  LE+H+S+IL S+Q NPL HL+IFPYY+NYLKL  LEF +L +
Sbjct: 61  VQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQ 120

Query: 121 HAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSR 180
           +     P  VAF+GSG LP +SI++A  +L  T FHNFD+DPSAN+ A+ LV+ D D+S+
Sbjct: 121 NLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 181 RMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYG 240
           RM FHT DIM+V  S L  F+VVFLAALVGM +EEKVKVIEHL K+MA GA+LMLRSA+G
Sbjct: 181 RMFFHTVDIMDVTES-LKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHG 240

Query: 241 ARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
            RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 PRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Spg003427 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 4.5e-88
Identity = 167/275 (60.73%), Postives = 214/275 (77.82%), Query Frame = 0


Query: 3   CQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSAVQ 62
           CQ   LV K+ +LY KIS LE+LKP +DV++LF QLV  C PP P+   IDV+K+   +Q
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN---IDVTKMSENIQ 63

Query: 63  NMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSIFPYYSNYLKLTHLEFSILTRHA 122
            MRS+LIK+CGEAE  LEHH+S+IL S + NPL HL++FPYY+NYLKL+ LEF +L ++ 
Sbjct: 64  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 123

Query: 123 PHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSRRM 182
               P  VAF+GSG LP +S+++A  +L  + FHNFD+DPSAN  A  LV+ D DLS+RM
Sbjct: 124 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 183

Query: 183 TFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYGAR 242
            FHT DIM+V  S  G F+VVFLAALVGM+++EKVKV+EHL K+M+ GALLMLRSA+G R
Sbjct: 184 FFHTVDIMDVTESLKG-FDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPR 243

Query: 243 AFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           AFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 AFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Spg003427 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 6.6e-87
Identity = 165/280 (58.93%), Postives = 221/280 (78.93%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           M CQ  L+V+++ +LY +IS L+SLKPSK+V++LF QLV  C P   +   IDV+ +C  
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTN---IDVTNMCEE 60

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILAS---HQNPLDHLSIFPYYSNYLKLTHLEFSIL 120
           V++MR++LIKLCGEAE  LE H+STIL S    QNPLDHL IFPYYSNYLKL  LEF +L
Sbjct: 61  VKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL 120

Query: 121 TRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADL 180
           ++H+ H  P+K+AFVGSG +P +SI++A  +LP T FHNFD+D  AN  A+ LV+ D DL
Sbjct: 121 SQHSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDL 180

Query: 181 SRRMTFHTTDIMEVNASE-LGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRS 240
           S+RM FHTTD++  NA+E L +++VVFLAALVGM++E KVK IEHL K+MA GA+LMLRS
Sbjct: 181 SKRMIFHTTDVL--NATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           A+  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 AHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Spg003427 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 9.5e-86
Identity = 163/279 (58.42%), Postives = 222/279 (79.57%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKL-CS 60
           M C+  L+V+++ +LY++IS LESLKPSK+V++LF QLV  C P   +   IDV+++   
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTN---IDVTEIHDE 60

Query: 61  AVQNMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSIFPYYSNYLKLTHLEFSILT 120
            V++MRSHLIKLCGEAE  LE H+S IL S + NPL+HL IFPYY+NYLKL  LEF +L+
Sbjct: 61  KVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLS 120

Query: 121 RHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLS 180
           +H  H  P+KVAF+GSG +P +SI++A  +LP T FHNFD+D  AN  A+ LV+ D+DLS
Sbjct: 121 QHTTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLS 180

Query: 181 RRMTFHTTDIMEVNASE-LGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSA 240
           +RM FHTTD++  NA E L +++VVFLAALVGM++E KVK IEHL K+MA GA++MLRSA
Sbjct: 181 KRMIFHTTDVL--NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           +G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 HGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Spg003427 vs. ExPASy TrEMBL
Match: A0A6J1DTD8 (probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 6.7e-127
Identity = 242/277 (87.36%), Postives = 253/277 (91.34%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           MCCQ +LLVQKVSELYHKISTL+SLKPSKDVNSLFSQLVL C+PPPPSPPPIDVSKL SA
Sbjct: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSILTRH 120
           VQ MRSHLIKLCGEAEALLEHHYS+ILASH+NPL++LSIFPYYSNYLKL HLEFSIL R 
Sbjct: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILER- 120

Query: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSRR 180
             HA PSKVAFVGSGALPF+SIIMA KYLP TEFHNFDVDPSANAKA ALVAGD DL RR
Sbjct: 121 --HAQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERR 180

Query: 181 MTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYGA 240
           M FHT DIMEV   EL  F+VVFLAALVGMEREEKVKVIEHLGKNM GGALLMLRSAYGA
Sbjct: 181 MVFHTADIMEV-TGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240

Query: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           RAFLYPVVEA DL+GFEILTVFHPTDEVINS+VLARL
Sbjct: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of Spg003427 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 3.3e-126
Identity = 239/281 (85.05%), Postives = 254/281 (90.39%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLV-LICSPPPPSPPPIDVSKLCS 60
           MCCQ LLLVQK+SELYHKISTLESLKPSK VNSLFSQLV LIC+   PS   IDVS LCS
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICT---PSSSSIDVSTLCS 60

Query: 61  AVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSILTR 120
            +Q MRSHLIKLCGEAE+LLEHHYSTIL+SHQNPLDHLS+FPYYSNYLKLTHLEFSIL  
Sbjct: 61  TIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNH 120

Query: 121 HAPHA---PPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDAD 180
           HAPH+   PPSKVAFVGSGALPFSSIIMALKY P TEFHNFD+DPSANAKA+ LVAGD +
Sbjct: 121 HAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVE 180

Query: 181 LSRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRS 240
           LSRRM FHTTDIM+V + EL EFEVVFLAALVGMEREEK KVIEHL KNM+GGALLMLRS
Sbjct: 181 LSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           AYGARAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 241 AYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of Spg003427 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.7e-126
Identity = 238/282 (84.40%), Postives = 255/282 (90.43%), Query Frame = 0


Query: 1   MCC--QGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICS---PPPPSPPPIDVS 60
           MCC  +GLLLVQKVSELYHKISTL+SL+PSK VNSLFSQLVL C+   PPPPSPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLS+FPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDA 180
           ILTR+   + PSKVAF+GSGALPFSSIIMALKYLPTTEFHNFDVD SANAKATALVA D 
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLSRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLR 240
           DLSRRM+FHTTDIM+V +  LGEFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Spg003427 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.7e-126
Identity = 241/280 (86.07%), Postives = 253/280 (90.36%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLV-LICSPP--PPSPPPIDVSKL 60
           MCCQ LLLVQK+SELYHKISTLESLKPSK VNSLFSQLV LIC+PP    SP  IDVS L
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60

Query: 61  CSAVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFSIL 120
           CS++Q MRS+LIKLCGEAE+LLEHHYSTIL+SHQNPLDHLS+FPYYSNYLKLTHLEFSIL
Sbjct: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 TRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADL 180
             H    PPSKVAFVGSGALPFSSIIMALKYLP TEFHNFD+DPSANAKA+ LVAGD +L
Sbjct: 121 NHHIT-PPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVEL 180

Query: 181 SRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSA 240
           SRRM FHTTDIMEV + EL EFEVVFLAALVGMEREEK KVIEHL KNM GGALLMLRSA
Sbjct: 181 SRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of Spg003427 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.7e-125
Identity = 238/282 (84.40%), Postives = 253/282 (89.72%), Query Frame = 0


Query: 1   MCC--QGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICS---PPPPSPPPIDVS 60
           MCC  +GLLLVQKVSELYHKISTL+SL+PSK VNSLFSQLVL C+   PPPPSPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSIFPYYSNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLS+FPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDA 180
           ILTR+   A PSKVAF+GSGALPFSSIIMALKYLPTTEFHNFDVD SANAKAT LVA D 
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLSRRMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLR 240
           DLSRRMTFHTTDIM+V +  L EFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Spg003427 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 327.4 bits (838), Expect = 1.1e-89
Identity = 170/277 (61.37%), Postives = 216/277 (77.98%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           M CQ   LVQ + +LY KIS LESLKPS+DVN LF QLV  C PP P+   IDV+K+C  
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN---IDVTKMCDR 60

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSIFPYYSNYLKLTHLEFSILTR 120
           VQ +R +LIK+CG AE  LE+H+S+IL S+Q NPL HL+IFPYY+NYLKL  LEF +L +
Sbjct: 61  VQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQ 120

Query: 121 HAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSR 180
           +     P  VAF+GSG LP +SI++A  +L  T FHNFD+DPSAN+ A+ LV+ D D+S+
Sbjct: 121 NLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 181 RMTFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYG 240
           RM FHT DIM+V  S L  F+VVFLAALVGM +EEKVKVIEHL K+MA GA+LMLRSA+G
Sbjct: 181 RMFFHTVDIMDVTES-LKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHG 240

Query: 241 ARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
            RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 PRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Spg003427 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 325.9 bits (834), Expect = 3.2e-89
Identity = 167/275 (60.73%), Postives = 214/275 (77.82%), Query Frame = 0


Query: 3   CQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSAVQ 62
           CQ   LV K+ +LY KIS LE+LKP +DV++LF QLV  C PP P+   IDV+K+   +Q
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN---IDVTKMSENIQ 63

Query: 63  NMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSIFPYYSNYLKLTHLEFSILTRHA 122
            MRS+LIK+CGEAE  LEHH+S+IL S + NPL HL++FPYY+NYLKL+ LEF +L ++ 
Sbjct: 64  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 123

Query: 123 PHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLSRRM 182
               P  VAF+GSG LP +S+++A  +L  + FHNFD+DPSAN  A  LV+ D DLS+RM
Sbjct: 124 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 183

Query: 183 TFHTTDIMEVNASELGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSAYGAR 242
            FHT DIM+V  S  G F+VVFLAALVGM+++EKVKV+EHL K+M+ GALLMLRSA+G R
Sbjct: 184 FFHTVDIMDVTESLKG-FDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPR 243

Query: 243 AFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           AFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 AFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Spg003427 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 322.0 bits (824), Expect = 4.7e-88
Identity = 165/280 (58.93%), Postives = 221/280 (78.93%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKLCSA 60
           M CQ  L+V+++ +LY +IS L+SLKPSK+V++LF QLV  C P   +   IDV+ +C  
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTN---IDVTNMCEE 60

Query: 61  VQNMRSHLIKLCGEAEALLEHHYSTILAS---HQNPLDHLSIFPYYSNYLKLTHLEFSIL 120
           V++MR++LIKLCGEAE  LE H+STIL S    QNPLDHL IFPYYSNYLKL  LEF +L
Sbjct: 61  VKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL 120

Query: 121 TRHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADL 180
           ++H+ H  P+K+AFVGSG +P +SI++A  +LP T FHNFD+D  AN  A+ LV+ D DL
Sbjct: 121 SQHSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDL 180

Query: 181 SRRMTFHTTDIMEVNASE-LGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRS 240
           S+RM FHTTD++  NA+E L +++VVFLAALVGM++E KVK IEHL K+MA GA+LMLRS
Sbjct: 181 SKRMIFHTTDVL--NATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           A+  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 AHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Spg003427 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 318.2 bits (814), Expect = 6.7e-87
Identity = 163/279 (58.42%), Postives = 222/279 (79.57%), Query Frame = 0


Query: 1   MCCQGLLLVQKVSELYHKISTLESLKPSKDVNSLFSQLVLICSPPPPSPPPIDVSKL-CS 60
           M C+  L+V+++ +LY++IS LESLKPSK+V++LF QLV  C P   +   IDV+++   
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTN---IDVTEIHDE 60

Query: 61  AVQNMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSIFPYYSNYLKLTHLEFSILT 120
            V++MRSHLIKLCGEAE  LE H+S IL S + NPL+HL IFPYY+NYLKL  LEF +L+
Sbjct: 61  KVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLS 120

Query: 121 RHAPHAPPSKVAFVGSGALPFSSIIMALKYLPTTEFHNFDVDPSANAKATALVAGDADLS 180
           +H  H  P+KVAF+GSG +P +SI++A  +LP T FHNFD+D  AN  A+ LV+ D+DLS
Sbjct: 121 QHTTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLS 180

Query: 181 RRMTFHTTDIMEVNASE-LGEFEVVFLAALVGMEREEKVKVIEHLGKNMAGGALLMLRSA 240
           +RM FHTTD++  NA E L +++VVFLAALVGM++E KVK IEHL K+MA GA++MLRSA
Sbjct: 181 KRMIFHTTDVL--NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           +G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 HGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Spg003427 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 62.4 bits (150), Expect = 6.6e-10
Identity = 30/53 (56.60%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 226 MAGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHP--TDEVINSIVLAR 277
           M  GALLMLRSA G R+FLY  V+ CDL+GFE+L ++HP  +D  +NS+++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of Spg003427 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891237.1	3.3e-136	90.61	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_022156409.1	1.4e-126	87.36	probable nicotianamine synthase 4 [Momordica charantia]	[more]
KAG7021049.1	6.9e-126	84.04	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_004144812.1	6.9e-126	85.05	nicotianamine synthase 1 [Cucumis sativus]	[more]
XP_022938003.1	1.2e-125	84.40	nicotianamine synthase-like [Cucurbita moschata]	[more]

BLAST of Spg003427 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	6.3e-90	60.14	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
O80483	1.6e-88	61.37	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9C7X5	4.5e-88	60.73	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9FF79	6.6e-87	58.93	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	9.5e-86	58.42	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Spg003427 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DTD8	6.7e-127	87.36	probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309...	[more]
A0A0A0LJ09	3.3e-126	85.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]
A0A6J1FHL8	5.7e-126	84.40	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]
A0A1S3BXJ6	5.7e-126	86.07	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A6J1HS54	3.7e-125	84.40	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]

BLAST of Spg003427 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09240.1	1.1e-89	61.37	nicotianamine synthase 3	[more]
AT1G56430.1	3.2e-89	60.73	nicotianamine synthase 4	[more]
AT5G04950.1	4.7e-88	58.93	nicotianamine synthase 1	[more]
AT5G56080.1	6.7e-87	58.42	nicotianamine synthase 2	[more]
AT4G26483.1	6.6e-10	56.60	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 7..276 e-value: 1.4E-113 score: 378.8
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..276
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..277 score: 53.465694
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 5..277 e-value: 8.6E-62 score: 210.7
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 1..276
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 83..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003427.1	Spg003427.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity