Spg003388 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003388
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionHistidine kinase CKI1-like
Locationscaffold4: 43923325 .. 43928629 (+)
RNA-Seq ExpressionSpg003388
SyntenySpg003388
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCAAACATATTCATTCTCATCTCCTCGTGAACAATTACAATGCTACCTCTGAGATGTCATTGGAGATGGAGAAAATATCGACATCGATCCAACCAATTTATGTGTCGACGACAAATTTGGCTAAAATTCTTGGCTCTTCTTTCAATGGAACTCAAATTTCATTTTTGGAAGTGAAATCTAAGGTTAGTTACTAATTGATTCCTTGTCTTTTATGTCTAACTACAATACCCAAAAAGGAGAAGAAAAAGAAAATTTGATTTTAGATGGTTAACTCCATTATTACTTATTGAGAATTTGAAACATTAGATAATGTCAATTATGTCCTTATACATTGATTTTTTTTTTTTTTTAAAAAAAAGACCTTATACATTGAAATTATGATTCTCGTAACTAAGACTCAATTGGATAACAATTTGATTTTTGAAAATTATGAGTTTTTTTTTTTTTTTTTTTTGGAAACCCCTTACAATGGTTTTTATCATTGTTAAAGACACATTTGAATTTTTAGGCAAATTCCAAAAACAAAACTTGACTTTGTTTTTGAAAACATGGAAAGAAAGTAGATAACAAAATAATGAAAGTAGTATTTATAACTTTAATTTTCAAAAACAAAAATCTAAGGCCTTGTTTGATAACCATTTGTTTTTTGGTTTTTGAAAATTAAGGTTGTAAACACTACTCCCACTTGTAACTTTCTTTGTTTTTTCATCTACCTTGTTTCCATGTTTTCAAAAATCAAGCCAATTTTTGAAAACTAAGACCCTATTTGATAACCATTTGGTTTTTGGTTTTTAGTTTTTCAAAATTAGGCTTGTTTTTTTCCAATTTCCCTACCATGGTTTTCATCTTTGTCAATGACACATTTGAATTCCTAGCCAAATTCTAAAAACAAAAACAAGTTTTTGCAAATTACTTTTTTTAGTTTTCAAATTTTGGTTTTTAAAAACAAGGAAAGAAAGGTATTAGAAAAACAAAGAAGCTTGAAGGTAGAAGTAGGATTTATAAGCTTAATTTGCAAAAACCAAATGGTTATCAAACAAGACCTAAAAAAAGTAGTTTTCAAAAACTTGTTTTTGTTTTTTGAATTGGGCTAGGAATTCAAATATATTATTGACAAAGATGAAAGCCACAGTAAGGAAGGGAGAAAACAACATAATTTTCAAAAACCAAATGTTTATCAAATGAGACCGTAGTTTCAAAAAAAAATGAAGTGACAAAAAAAAAACGTCATTTTTTTGAAAAAGACATCGAAATTCTTTTATAAAATTTGTTACTGTATTTCATCTTCATTACATCATTTGTTAGTAAAAATGTACCAAATTGGCCAAAGTTTAGGGGTGTAATTAGCAACTTCACTACATTCAACTATTGATGCTTTTAATTTCTTTTGATCTTTCAGATTGCTCCTACGTTATTTCAAGGATTTTCAACCATTCCATACCTGACTCAAATTTCCTATATTGGGATGGATGGTCTCTTCTTCTCATACTACACTGACAATAACCAAACTTTTGCAGTCTATGCTAACTCTACCTTCACTGCCAGATTCTATCCTCATCCAAGAAGGGAATACACTTGGCTGACCCAATTGGTAAACTCTAGCACAGGAGAAACATATGGGGATATGGCTCAGACCCTCCCCTTAGCCACTAGCAACACAAGCTGGGTTCGAGACGCCTTGAATAGTAACCGGGGATGTGCATCTATAGGGCCAAAATGGGGATCAGATCATGAACGTTTGTTGCTCTACACATATAAAGTTAATGAAAGTAATGGAGTTGTCTCCTTCGGATTTTCAATCAAAGCATTCGTCGATCTTTTCTTCACGAGCATTGAACGTCAAGGAGGGAGATTGTACCTGGCAAACACTGAAGGAGAAATTCTTGTCACAGGGTTTCAGAACATTAGGATGGTTCTTACTAATGGTTCGGCTTCATTTCAGTTCTTGAATCCAAATGGTGATCAAATTGCTCGAGTCGGGAACATCTCGTGCTTGCCTAGGAAAGAAGATTTTGATGCAAGGGATTCTTTCTTTAATCTTCTGGGTACAAAGTATATGATATATTGCTCTCCACTTGAGATATTGGGCGTGCAGTTGGTAAGTCATCATGCTCCAACAAGAACATATATCCTGAAATCAGTAAACATTGATAAGAAATGATTTTTTTTGTCTCTTCCCTATGTTCTTATCATCCTGAGGCATTTAGATGGCATAAACTAAAATATCTTAATCTCATATTTTTATTGACTATGTTAAGTCCAAATGCATACACACATTATTTTTAATGTCTCTTACCTGTGTTTCTTATTTCAGGTCTATTCATTAGTATTGCCTCAGAAAGAGTTAGCTAGCCTTGTCTACAAGAGTAGCAGAGTGGGACTAATTCTCCTTATACTAATAATGACTACCACAGTCATCTCCATTTTTGGTTTTGTGTTCATAGTCATTAGAGCAGCAAAAAGAGAGATGTATTTATGTGCCAAACTCATTCAACAAATGGAAGCAACTCAACAGGCAGAGAGAAAGAGCATGAACAAGAGTGTTGCTTTCACACAAGCAAGCCATGACATTCGCGCTTCTTTGGCAGGCATTATTGGTTTGATTGAGATATGCTATAATGAAGCTGTCCCAGGTTCAGAGTTAGACATAAACCTAAAACAGATGGATGGTTGTACAAAGGATCTACTAGGTAATTCAAATTCAAAACTTGAACTCTTATCTCTTTGATTGTCAAACATATAAAAAATTTGGTTATAATCCATTTCAGGCATATTGAACTCCATTCTGGATACAAGCAAGATTGAAGCAGGAAAGATACAGCTTGAGGAAGAAGAGTTTCATTTGGGTCAACTTCTTGAGGATGTGGTAGATTTGTATCATCCAGTGGGTATGAAGAAAGGAATAGACCTAGTGTTGGATCCTTACGATGGCTCAGTTATCAAGTTTTCACAAGTGAAAGGTGATAGAGGAAAGCTTAAACAAGTTTTGTGCAATTTACTGAGCAATGCTGTTAAGTTCACTTCTGAAGGGCAAGTAACTGTTCGAGCATGGGTCAAGAACTTACCTGATATGCAGAGTAATATGATTTCTTCGAAGCACAATGGTGAAATATTGAAGCATTTATCCTTTTTATTATGCAAGAACACAGAAAGGTTTCGAGAACAACAAGCCACAGATAATGGAGTACATTTGAATTCTGATTGTATGGAATTTACATTTGAGATAGATGACACAGGGAAAGGCATTCCTAAAGAGAAGCGGAAATTGGTTTTTGAGAACTATGTCCAAGTCAAAGAAACAGCTTTGGGACAAGGAGGAACTGGCTTGGGACTTGGCATTGTTCAATCTCTGGTATGTAACTTCAACTTCAAAGATAAACTGGTCTATCGTTTTTCCTCACACCTTGCTAATTTGTAACTAAATGTTCATATTGAAGCTTATCTCATTATTTAGATAAAGACATTTCAAGTACAATTCTTTGAATTATTGAAGGTACGCTTGATGGGAGGAGATATAGCAATTTTAGATAAAGAGATTGGAGAAAAGGGAACATGCTTCAGGTTCAGTGTTCTTCTTACCGTTTCAGTGGGCAATATCAACTCTGGTGAAGTCACATGTCAATCATCACCTACCTCAAGACTAACCTTTCAGGCCCCTAGTCCCAGTCTCCATTCCCCTAGAATGATCCGAACTACTGGTTCAAAAACTGAAACATCTCGTGTCATTCTCTTAATTCGAAATGATCAACGAAGAATGGTATGCAAGAAATTCATGGAAACTCTTGGTGTAAAAGTACTGGCAATGAAACAATGGGAGCAACTCCAAGTCACTCTACAGAAAATATTGGAGAAACAGAGCCATTCTAGGCACAGCTCAAGAGGAAGGTCAGGTAATAGTTCACCGAGTGACTGCCTAAGCAAATCAACATCTGGTGACTCTGGCAATGGGCCGAATATTGATGTTTCTTTGGGTGCAATGAAAGATGAGACAAATTACTTGCTTTCAGTATTCAAAAAGACTAGTCCCAGAGGTGGAATTAGCTTCATCTTGATTGTAATTGATGCCAGTGCAGGACCATTTAGAGAAATATGTAATATGGTGGCTAGTTTTAGAACTGGACTTCAAGATGCCTATTGTAAGGTTGTTTGGCTGCTTGAGAATCAAATGTCACGCATCATCAACCACAAGGGGCTAGACTCGAGCGTCTTCGAGTTGAATGATGTTGTTATATCCAAACCTTTTCATGGTTCTCGTTTGTATGAAGTGATAAAACTTCTTCCAGAGTTCGGAGGCACATTACAAAGTAGAGAAAGTAGTAGATTATATCAGATTGGGAGTGTTTCAAAAGATCCAAGTTCATCACTGTACCAATATCATAGTAAGGCCAAGGAGGGGAATTCACCAATTTTTAGAGGGCAAATTGCAACAAGAGTACAACAAGACAAAAAATCAAGTAGTGGGACCTCCCCAAAGAATTTGTCATTGAATCAAATTCATTCTTGTTTGGGCTCAAAGTCCAGAATTTCACCAGTTGTTGGGCAACAAAGTCGACACCAAGAACTTAGAGAAGAGAAATATGAAAACTTGAATGGTGAAAAACCTCTAAGTGGTAAAAAGATCTTGGTTGCCGAGGACAATCTGGTGTTGCAAAAACTAGCTAGATTGAACCTTGAAAGACTTGGTGCAACTATTGAAATATGTGAAAATGGAGAGGAAGCTTTGGAGCTTGTTTGCAGTGGCTTAGGCAATCAGCGAAAACATGGTGCTTCAAATACTCTTCCTTACGATTTCATACTAATGGACTGTGAGGTAAGAAGTTATCAACTGTCTTTTTCTTTCGTTCTTTCTTTCTTTCTTTTTTTTCTTTTTGGGATAAGAATAACTATATACTTACTATTATCTTAGGAAACAAAGATAAAACAAGAAAAACAAACAGTGTGGCTGTTAAAACTATAATAACAATGCTCTTAATATCATTTCATGTTTCCTACTTTCAACTAGAACATGTATGCAATTGATTAAGTTTTCACCTTCCTGCAAGAATTTAAGTCCATTTGAGATTTATTTTTGTACGAAATTGCATATGGTAGATGCCAGTTATGGATGGATATGAAGCAACTAGACAGATAAGGAAGATGGAAAGATATCACAACACCCACATTCCAATCATTGCACTGACTGCCCATACAACAAGAGAAGAAGCAAGGAGGACAATTGAGGCTGGAATGGACGTGCATTTAGGCAAGCCACTGAGAAAAGAGAACCTACTAGAAGTCATTGCATGTATCCATAGTAAATAA

mRNA sequence

ATGATCAAACATATTCATTCTCATCTCCTCGTGAACAATTACAATGCTACCTCTGAGATGTCATTGGAGATGGAGAAAATATCGACATCGATCCAACCAATTTATGTGTCGACGACAAATTTGGCTAAAATTCTTGGCTCTTCTTTCAATGGAACTCAAATTTCATTTTTGGAAGTGAAATCTAAGATTGCTCCTACGTTATTTCAAGGATTTTCAACCATTCCATACCTGACTCAAATTTCCTATATTGGGATGGATGGTCTCTTCTTCTCATACTACACTGACAATAACCAAACTTTTGCAGTCTATGCTAACTCTACCTTCACTGCCAGATTCTATCCTCATCCAAGAAGGGAATACACTTGGCTGACCCAATTGGTAAACTCTAGCACAGGAGAAACATATGGGGATATGGCTCAGACCCTCCCCTTAGCCACTAGCAACACAAGCTGGGTTCGAGACGCCTTGAATAGTAACCGGGGATGTGCATCTATAGGGCCAAAATGGGGATCAGATCATGAACGTTTGTTGCTCTACACATATAAAGTTAATGAAAGTAATGGAGTTGTCTCCTTCGGATTTTCAATCAAAGCATTCGTCGATCTTTTCTTCACGAGCATTGAACGTCAAGGAGGGAGATTGTACCTGGCAAACACTGAAGGAGAAATTCTTGTCACAGGGTTTCAGAACATTAGGATGGTTCTTACTAATGGTTCGGCTTCATTTCAGTTCTTGAATCCAAATGGTGATCAAATTGCTCGAGTCGGGAACATCTCGTGCTTGCCTAGGAAAGAAGATTTTGATGCAAGGGATTCTTTCTTTAATCTTCTGGGTACAAAGTATATGATATATTGCTCTCCACTTGAGATATTGGGCGTGCAGTTGGTCTATTCATTAGTATTGCCTCAGAAAGAGTTAGCTAGCCTTGTCTACAAGAGTAGCAGAGTGGGACTAATTCTCCTTATACTAATAATGACTACCACAGTCATCTCCATTTTTGGTTTTGTGTTCATAGTCATTAGAGCAGCAAAAAGAGAGATGTATTTATGTGCCAAACTCATTCAACAAATGGAAGCAACTCAACAGGCAGAGAGAAAGAGCATGAACAAGAGTGTTGCTTTCACACAAGCAAGCCATGACATTCGCGCTTCTTTGGCAGGCATTATTGGTTTGATTGAGATATGCTATAATGAAGCTGTCCCAGGTTCAGAGTTAGACATAAACCTAAAACAGATGGATGGTTGTACAAAGGATCTACTAGGCATATTGAACTCCATTCTGGATACAAGCAAGATTGAAGCAGGAAAGATACAGCTTGAGGAAGAAGAGTTTCATTTGGGTCAACTTCTTGAGGATGTGGTAGATTTGTATCATCCAGTGGGTATGAAGAAAGGAATAGACCTAGTGTTGGATCCTTACGATGGCTCAGTTATCAAGTTTTCACAAGTGAAAGGTGATAGAGGAAAGCTTAAACAAGTTTTGTGCAATTTACTGAGCAATGCTGTTAAGTTCACTTCTGAAGGGCAAGTAACTGTTCGAGCATGGGTCAAGAACTTACCTGATATGCAGAGTAATATGATTTCTTCGAAGCACAATGGTGAAATATTGAAGCATTTATCCTTTTTATTATGCAAGAACACAGAAAGGTTTCGAGAACAACAAGCCACAGATAATGGAGTACATTTGAATTCTGATTGTATGGAATTTACATTTGAGATAGATGACACAGGGAAAGGCATTCCTAAAGAGAAGCGGAAATTGGTTTTTGAGAACTATGTCCAAGTCAAAGAAACAGCTTTGGGACAAGGAGGAACTGGCTTGGGACTTGGCATTGTTCAATCTCTGGTACGCTTGATGGGAGGAGATATAGCAATTTTAGATAAAGAGATTGGAGAAAAGGGAACATGCTTCAGGTTCAGTGTTCTTCTTACCGTTTCAGTGGGCAATATCAACTCTGGTGAAGTCACATGTCAATCATCACCTACCTCAAGACTAACCTTTCAGGCCCCTAGTCCCAGTCTCCATTCCCCTAGAATGATCCGAACTACTGGTTCAAAAACTGAAACATCTCGTGTCATTCTCTTAATTCGAAATGATCAACGAAGAATGGTATGCAAGAAATTCATGGAAACTCTTGGTGTAAAAGTACTGGCAATGAAACAATGGGAGCAACTCCAAGTCACTCTACAGAAAATATTGGAGAAACAGAGCCATTCTAGGCACAGCTCAAGAGGAAGGTCAGGTAATAGTTCACCGAGTGACTGCCTAAGCAAATCAACATCTGGTGACTCTGGCAATGGGCCGAATATTGATGTTTCTTTGGGTGCAATGAAAGATGAGACAAATTACTTGCTTTCAGTATTCAAAAAGACTAGTCCCAGAGGTGGAATTAGCTTCATCTTGATTGTAATTGATGCCAGTGCAGGACCATTTAGAGAAATATGTAATATGGTGGCTAGTTTTAGAACTGGACTTCAAGATGCCTATTGTAAGGTTGTTTGGCTGCTTGAGAATCAAATGTCACGCATCATCAACCACAAGGGGCTAGACTCGAGCGTCTTCGAGTTGAATGATGTTGTTATATCCAAACCTTTTCATGGTTCTCGTTTGTATGAAGTGATAAAACTTCTTCCAGAGTTCGGAGGCACATTACAAAGTAGAGAAAGTAGTAGATTATATCAGATTGGGAGTGTTTCAAAAGATCCAAGTTCATCACTGTACCAATATCATAGTAAGGCCAAGGAGGGGAATTCACCAATTTTTAGAGGGCAAATTGCAACAAGAGTACAACAAGACAAAAAATCAAGTAGTGGGACCTCCCCAAAGAATTTGTCATTGAATCAAATTCATTCTTGTTTGGGCTCAAAGTCCAGAATTTCACCAGTTGTTGGGCAACAAAGTCGACACCAAGAACTTAGAGAAGAGAAATATGAAAACTTGAATGGTGAAAAACCTCTAAGTGGTAAAAAGATCTTGGTTGCCGAGGACAATCTGGTGTTGCAAAAACTAGCTAGATTGAACCTTGAAAGACTTGGTGCAACTATTGAAATATGTGAAAATGGAGAGGAAGCTTTGGAGCTTGTTTGCAGTGGCTTAGGCAATCAGCGAAAACATGGTGCTTCAAATACTCTTCCTTACGATTTCATACTAATGGACTGTGAGATGCCAGTTATGGATGGATATGAAGCAACTAGACAGATAAGGAAGATGGAAAGATATCACAACACCCACATTCCAATCATTGCACTGACTGCCCATACAACAAGAGAAGAAGCAAGGAGGACAATTGAGGCTGGAATGGACGTGCATTTAGGCAAGCCACTGAGAAAAGAGAACCTACTAGAAGTCATTGCATGTATCCATAGTAAATAA

Coding sequence (CDS)

ATGATCAAACATATTCATTCTCATCTCCTCGTGAACAATTACAATGCTACCTCTGAGATGTCATTGGAGATGGAGAAAATATCGACATCGATCCAACCAATTTATGTGTCGACGACAAATTTGGCTAAAATTCTTGGCTCTTCTTTCAATGGAACTCAAATTTCATTTTTGGAAGTGAAATCTAAGATTGCTCCTACGTTATTTCAAGGATTTTCAACCATTCCATACCTGACTCAAATTTCCTATATTGGGATGGATGGTCTCTTCTTCTCATACTACACTGACAATAACCAAACTTTTGCAGTCTATGCTAACTCTACCTTCACTGCCAGATTCTATCCTCATCCAAGAAGGGAATACACTTGGCTGACCCAATTGGTAAACTCTAGCACAGGAGAAACATATGGGGATATGGCTCAGACCCTCCCCTTAGCCACTAGCAACACAAGCTGGGTTCGAGACGCCTTGAATAGTAACCGGGGATGTGCATCTATAGGGCCAAAATGGGGATCAGATCATGAACGTTTGTTGCTCTACACATATAAAGTTAATGAAAGTAATGGAGTTGTCTCCTTCGGATTTTCAATCAAAGCATTCGTCGATCTTTTCTTCACGAGCATTGAACGTCAAGGAGGGAGATTGTACCTGGCAAACACTGAAGGAGAAATTCTTGTCACAGGGTTTCAGAACATTAGGATGGTTCTTACTAATGGTTCGGCTTCATTTCAGTTCTTGAATCCAAATGGTGATCAAATTGCTCGAGTCGGGAACATCTCGTGCTTGCCTAGGAAAGAAGATTTTGATGCAAGGGATTCTTTCTTTAATCTTCTGGGTACAAAGTATATGATATATTGCTCTCCACTTGAGATATTGGGCGTGCAGTTGGTCTATTCATTAGTATTGCCTCAGAAAGAGTTAGCTAGCCTTGTCTACAAGAGTAGCAGAGTGGGACTAATTCTCCTTATACTAATAATGACTACCACAGTCATCTCCATTTTTGGTTTTGTGTTCATAGTCATTAGAGCAGCAAAAAGAGAGATGTATTTATGTGCCAAACTCATTCAACAAATGGAAGCAACTCAACAGGCAGAGAGAAAGAGCATGAACAAGAGTGTTGCTTTCACACAAGCAAGCCATGACATTCGCGCTTCTTTGGCAGGCATTATTGGTTTGATTGAGATATGCTATAATGAAGCTGTCCCAGGTTCAGAGTTAGACATAAACCTAAAACAGATGGATGGTTGTACAAAGGATCTACTAGGCATATTGAACTCCATTCTGGATACAAGCAAGATTGAAGCAGGAAAGATACAGCTTGAGGAAGAAGAGTTTCATTTGGGTCAACTTCTTGAGGATGTGGTAGATTTGTATCATCCAGTGGGTATGAAGAAAGGAATAGACCTAGTGTTGGATCCTTACGATGGCTCAGTTATCAAGTTTTCACAAGTGAAAGGTGATAGAGGAAAGCTTAAACAAGTTTTGTGCAATTTACTGAGCAATGCTGTTAAGTTCACTTCTGAAGGGCAAGTAACTGTTCGAGCATGGGTCAAGAACTTACCTGATATGCAGAGTAATATGATTTCTTCGAAGCACAATGGTGAAATATTGAAGCATTTATCCTTTTTATTATGCAAGAACACAGAAAGGTTTCGAGAACAACAAGCCACAGATAATGGAGTACATTTGAATTCTGATTGTATGGAATTTACATTTGAGATAGATGACACAGGGAAAGGCATTCCTAAAGAGAAGCGGAAATTGGTTTTTGAGAACTATGTCCAAGTCAAAGAAACAGCTTTGGGACAAGGAGGAACTGGCTTGGGACTTGGCATTGTTCAATCTCTGGTACGCTTGATGGGAGGAGATATAGCAATTTTAGATAAAGAGATTGGAGAAAAGGGAACATGCTTCAGGTTCAGTGTTCTTCTTACCGTTTCAGTGGGCAATATCAACTCTGGTGAAGTCACATGTCAATCATCACCTACCTCAAGACTAACCTTTCAGGCCCCTAGTCCCAGTCTCCATTCCCCTAGAATGATCCGAACTACTGGTTCAAAAACTGAAACATCTCGTGTCATTCTCTTAATTCGAAATGATCAACGAAGAATGGTATGCAAGAAATTCATGGAAACTCTTGGTGTAAAAGTACTGGCAATGAAACAATGGGAGCAACTCCAAGTCACTCTACAGAAAATATTGGAGAAACAGAGCCATTCTAGGCACAGCTCAAGAGGAAGGTCAGGTAATAGTTCACCGAGTGACTGCCTAAGCAAATCAACATCTGGTGACTCTGGCAATGGGCCGAATATTGATGTTTCTTTGGGTGCAATGAAAGATGAGACAAATTACTTGCTTTCAGTATTCAAAAAGACTAGTCCCAGAGGTGGAATTAGCTTCATCTTGATTGTAATTGATGCCAGTGCAGGACCATTTAGAGAAATATGTAATATGGTGGCTAGTTTTAGAACTGGACTTCAAGATGCCTATTGTAAGGTTGTTTGGCTGCTTGAGAATCAAATGTCACGCATCATCAACCACAAGGGGCTAGACTCGAGCGTCTTCGAGTTGAATGATGTTGTTATATCCAAACCTTTTCATGGTTCTCGTTTGTATGAAGTGATAAAACTTCTTCCAGAGTTCGGAGGCACATTACAAAGTAGAGAAAGTAGTAGATTATATCAGATTGGGAGTGTTTCAAAAGATCCAAGTTCATCACTGTACCAATATCATAGTAAGGCCAAGGAGGGGAATTCACCAATTTTTAGAGGGCAAATTGCAACAAGAGTACAACAAGACAAAAAATCAAGTAGTGGGACCTCCCCAAAGAATTTGTCATTGAATCAAATTCATTCTTGTTTGGGCTCAAAGTCCAGAATTTCACCAGTTGTTGGGCAACAAAGTCGACACCAAGAACTTAGAGAAGAGAAATATGAAAACTTGAATGGTGAAAAACCTCTAAGTGGTAAAAAGATCTTGGTTGCCGAGGACAATCTGGTGTTGCAAAAACTAGCTAGATTGAACCTTGAAAGACTTGGTGCAACTATTGAAATATGTGAAAATGGAGAGGAAGCTTTGGAGCTTGTTTGCAGTGGCTTAGGCAATCAGCGAAAACATGGTGCTTCAAATACTCTTCCTTACGATTTCATACTAATGGACTGTGAGATGCCAGTTATGGATGGATATGAAGCAACTAGACAGATAAGGAAGATGGAAAGATATCACAACACCCACATTCCAATCATTGCACTGACTGCCCATACAACAAGAGAAGAAGCAAGGAGGACAATTGAGGCTGGAATGGACGTGCATTTAGGCAAGCCACTGAGAAAAGAGAACCTACTAGAAGTCATTGCATGTATCCATAGTAAATAA

Protein sequence

MIKHIHSHLLVNNYNATSEMSLEMEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREYTWLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSFGFSIKAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSASFQFLNPNGDQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLVLPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSFLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRISPVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK
Homology
BLAST of Spg003388 vs. NCBI nr
Match: KAA0048070.1 (histidine kinase CKI1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1803.1 bits (4669), Expect = 0.0e+00
Identity = 919/1123 (81.83%), Postives = 1008/1123 (89.76%), Query Frame = 0

Query: 1    MIKHIHSHLLVNNYNATSEMSLEMEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVK 60
            MIKHI SH L NNYNATS+M  E+E+IS+SIQPIYVSTTN AK+L S FNGTQ+SF E+ 
Sbjct: 36   MIKHIQSHYLFNNYNATSQMPHEIEEISSSIQPIYVSTTNFAKLLDSYFNGTQVSFFELN 95

Query: 61   SKIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREY 120
            SKIAP LFQGFS IPYLTQISYIG DGLFFSYYTD NQTFAVYANSTFTA+FYP+PRREY
Sbjct: 96   SKIAPILFQGFSIIPYLTQISYIGTDGLFFSYYTDKNQTFAVYANSTFTAKFYPNPRREY 155

Query: 121  TWLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYT 180
            +WLTQ  NS+TGE YG+M + LPL TSNTSW RDALNSN+GCASIG KW S+HERL L T
Sbjct: 156  SWLTQSANSTTGELYGNMTEILPLVTSNTSWFRDALNSNQGCASIGTKWSSNHERLFLNT 215

Query: 181  YKVNESNGVVSFGFSIKAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSA 240
             +V  SNGVVSFGFS K F+DL FTS+ERQGGRLYLA  EGEILV G Q+I+MVL NGSA
Sbjct: 216  VRVTGSNGVVSFGFSFKTFIDLLFTSMERQGGRLYLATNEGEILVLGSQDIKMVLANGSA 275

Query: 241  SFQFLNPNGDQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLV 300
            +FQFLNPNG +IAR+GNISC  RKED D +DSFFNLLGT Y+IYC PLEILGVQLVYSLV
Sbjct: 276  TFQFLNPNGGEIARLGNISCQARKEDSDPKDSFFNLLGTDYIIYCYPLEILGVQLVYSLV 335

Query: 301  LPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEAT 360
            LPQKELASLVYKSSR+GLILLILIM  T+I++  FVFIVIRA KREM+LCAKLIQQMEAT
Sbjct: 336  LPQKELASLVYKSSRMGLILLILIMAITIITVLVFVFIVIRATKREMHLCAKLIQQMEAT 395

Query: 361  QQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLL 420
            QQAERKSMNKSVAFT+ASHDIRASLAGIIGLIEIC+NE+ PGSELDI+LKQMDGCTKDLL
Sbjct: 396  QQAERKSMNKSVAFTRASHDIRASLAGIIGLIEICHNESTPGSELDISLKQMDGCTKDLL 455

Query: 421  GILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKF 480
            GILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVG+KKGID+VLDPYDGS+IKF
Sbjct: 456  GILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGVKKGIDVVLDPYDGSIIKF 515

Query: 481  SQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLS 540
            SQVKGDRGKLKQ+LCNLLSNAVKFTSEGQVTVRAWVKNLP MQ+NMISS HN EILKH S
Sbjct: 516  SQVKGDRGKLKQILCNLLSNAVKFTSEGQVTVRAWVKNLPTMQNNMISSNHNDEILKHFS 575

Query: 541  FLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETAL 600
            FL+C NT  F+EQQA DNGV+LN  CMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETA 
Sbjct: 576  FLVC-NTNTFQEQQAMDNGVNLNPACMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETAF 635

Query: 601  GQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSS 660
            GQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLL V   N+++G+ T Q S
Sbjct: 636  GQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLIVLEDNVHTGDDTRQPS 695

Query: 661  PTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMK 720
            PTSRLTF APS SLHSPR IRTT SKTETSRVILLI+NDQRR++CKKF+E+LGVKVLAMK
Sbjct: 696  PTSRLTFWAPSTSLHSPRAIRTTSSKTETSRVILLIQNDQRRLICKKFLESLGVKVLAMK 755

Query: 721  QWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDE 780
            +WEQL  TLQKIL+KQSHS+H+SRGRSGNSSPSD LSKSTS DSGNG N+ VS GA KDE
Sbjct: 756  EWEQLLTTLQKILDKQSHSKHNSRGRSGNSSPSDYLSKSTSDDSGNGLNMHVSSGARKDE 815

Query: 781  TNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQM 840
            TNY LSVFKKT+ RGG SFILIVIDASAGPF+EICNMVA+FR GLQ AYCKVVWLLE QM
Sbjct: 816  TNYFLSVFKKTNLRGGNSFILIVIDASAGPFKEICNMVANFRRGLQGAYCKVVWLLEKQM 875

Query: 841  SRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSK 900
            SRI N KG+DS++ +LNDV IS+PFHGSRLYEVI+LLPEFGGTL++ ESS  Y  G+VSK
Sbjct: 876  SRISNDKGIDSNIDKLNDVFISRPFHGSRLYEVIRLLPEFGGTLETGESSTSYWSGNVSK 935

Query: 901  DPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRIS 960
            DPSSS YQ  SK KEGNSPIFRG I TRVQ++  S S TSPKNLS+NQIHSC+GSK+R S
Sbjct: 936  DPSSSAYQCQSKCKEGNSPIFRGHIETRVQKETTSRSWTSPKNLSMNQIHSCIGSKTRSS 995

Query: 961  PVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICEN 1020
            P+V Q+S HQE+REEKY++ +GEKPL GKK+LVAEDNL+LQKLARLNLERLGAT EICEN
Sbjct: 996  PIVEQKSLHQEIREEKYKHSSGEKPLCGKKVLVAEDNLLLQKLARLNLERLGATTEICEN 1055

Query: 1021 GEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPI 1080
            G+EALELVC+GLGNQRKHGASNTLPYD+ILMDCEMP+MDGYEATR+IRK+ERY+NTHIPI
Sbjct: 1056 GKEALELVCNGLGNQRKHGASNTLPYDYILMDCEMPIMDGYEATREIRKVERYYNTHIPI 1115

Query: 1081 IALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            IALTAHTT EEA +TIEAGMDVHLGKPLRKE LLE I CIHSK
Sbjct: 1116 IALTAHTTGEEAGKTIEAGMDVHLGKPLRKEKLLEAITCIHSK 1157

BLAST of Spg003388 vs. NCBI nr
Match: XP_004144811.2 (histidine kinase CKI1 [Cucumis sativus])

HSP 1 Score: 1782.7 bits (4616), Expect = 0.0e+00
Identity = 905/1104 (81.97%), Postives = 996/1104 (90.22%), Query Frame = 0

Query: 20   MSLEMEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQ 79
            M+ E+E ISTSIQP+YVSTTN AK+L S FNGTQ+SF E+ SKIAP LFQGFS IPYLTQ
Sbjct: 1    MTHEIENISTSIQPLYVSTTNFAKLLNSCFNGTQVSFFELNSKIAPMLFQGFSIIPYLTQ 60

Query: 80   ISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREYTWLTQLVNSSTGETYGDMA 139
            ISYIGMDGLFFSYYTD NQTFAVYANSTFTA+FYPHPRREY+WLTQL NS+TGE YG+M 
Sbjct: 61   ISYIGMDGLFFSYYTDKNQTFAVYANSTFTAKFYPHPRREYSWLTQLANSTTGELYGNMT 120

Query: 140  QTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSFGFSIKAF 199
            + LPL TSNTSW RDALNSN+GCASIG KW S+HERL L T +VN S GVVSFGFS K F
Sbjct: 121  EILPLVTSNTSWFRDALNSNQGCASIGTKWSSNHERLFLNTVRVNGSKGVVSFGFSFKTF 180

Query: 200  VDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSASFQFLNPNGDQIARVGNIS 259
            +DLFFTSIERQGGRLYLA+ EGEILV G Q+I+MVL NGSA+FQFLNPNG +IAR+GNIS
Sbjct: 181  IDLFFTSIERQGGRLYLASNEGEILVLGSQDIKMVLVNGSATFQFLNPNGGEIARLGNIS 240

Query: 260  CLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLVLPQKELASLVYKSSRVGLI 319
            C  RKEDFD +DSFFNLLGT Y IYC P+EILGVQLVYSLVLPQKELASL++KSSR+GLI
Sbjct: 241  CQARKEDFDPKDSFFNLLGTNYNIYCYPVEILGVQLVYSLVLPQKELASLIFKSSRMGLI 300

Query: 320  LLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQASH 379
            LLILIM  T+I++F FVFIVIR  KREM+LCAKLIQQMEATQQAERKSMNKSVAFT+ASH
Sbjct: 301  LLILIMAITIITVFIFVFIVIRGTKREMHLCAKLIQQMEATQQAERKSMNKSVAFTRASH 360

Query: 380  DIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGKIQL 439
            DIRASLAGIIGLIEIC+NE+ PGSELDI+LKQMDGCTKDLLGILNSILDTSKIEAGKIQL
Sbjct: 361  DIRASLAGIIGLIEICHNESTPGSELDISLKQMDGCTKDLLGILNSILDTSKIEAGKIQL 420

Query: 440  EEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQVKGDRGKLKQVLCNLLS 499
            EEEEFHLGQLLEDVVDLYHPVG+KKGIDLVLDPYDGS+IKFSQVKGDRGKLKQVLCNLLS
Sbjct: 421  EEEEFHLGQLLEDVVDLYHPVGVKKGIDLVLDPYDGSIIKFSQVKGDRGKLKQVLCNLLS 480

Query: 500  NAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSFLLCKNTERFREQQATDNG 559
            NAVKFTSEGQVTVRAWVKNLP MQ+NMISS HN EILK+ SFL+C NT  F+EQQA DNG
Sbjct: 481  NAVKFTSEGQVTVRAWVKNLPTMQNNMISSNHNDEILKYFSFLVC-NTSTFQEQQAMDNG 540

Query: 560  VHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALGQGGTGLGLGIVQSLVRLM 619
            V+LN  CMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETA GQGGTGLGLGIVQSLVRLM
Sbjct: 541  VNLNPGCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETAFGQGGTGLGLGIVQSLVRLM 600

Query: 620  GGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSSPTSRLTFQAPSPSLHSPRM 679
            GGDIAILDKEIGEKGTCFRFSVLL V   N+N+G+ T Q SPTS+LTF AP+ SLHSPR 
Sbjct: 601  GGDIAILDKEIGEKGTCFRFSVLLLVLEDNVNTGDDTRQPSPTSKLTFWAPTTSLHSPRA 660

Query: 680  IRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQWEQLQVTLQKILEKQSHS 739
            IRTT SKTETSRVILLI+NDQRR++CKKF+E+LGVKVLAMKQWEQL  TLQKIL+KQSHS
Sbjct: 661  IRTTSSKTETSRVILLIQNDQRRLICKKFLESLGVKVLAMKQWEQLLTTLQKILDKQSHS 720

Query: 740  RHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNYLLSVFKKTSPRGGISF 799
             H+SRGRSG SSPSDCLSKSTSGDS NG N+ +S GA KDETNY LSVFKKT+ RGG SF
Sbjct: 721  MHNSRGRSGTSSPSDCLSKSTSGDSRNGLNMHISSGARKDETNYFLSVFKKTNLRGGNSF 780

Query: 800  ILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQMSRIINHKGLDSSVFELNDV 859
            ILIVIDA AGPF+EICNMVA+FR  LQ ++CKVVWLLENQMSRI N KG+DS++++ NDV
Sbjct: 781  ILIVIDARAGPFKEICNMVANFRRRLQGSHCKVVWLLENQMSRISNDKGIDSNIYKSNDV 840

Query: 860  VISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSKDPSSSLYQYHSKAKEGNSP 919
             IS+PFHGSRLYEVI+LLPEFGGTL++ ESS  Y  G+VSKD SSS YQ  SK+KEGNSP
Sbjct: 841  FISRPFHGSRLYEVIRLLPEFGGTLETGESSTSYWSGNVSKDLSSSPYQCQSKSKEGNSP 900

Query: 920  IFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRISPVVGQQSRHQELREEKYEN 979
            IFRG I TRVQ++  SS+GTSPKNLS NQIHSC+GSK+R SP+V Q+S HQE+REEKY++
Sbjct: 901  IFRGHIETRVQKETTSSNGTSPKNLSTNQIHSCIGSKTRSSPIVEQKSLHQEIREEKYKH 960

Query: 980  LNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICENGEEALELVCSGLGNQRKHG 1039
             +GEKPLSGKK+LVAEDNLVLQKLARLNLERLGAT EICENG+EALELVC+GLGNQRKHG
Sbjct: 961  SSGEKPLSGKKVLVAEDNLVLQKLARLNLERLGATTEICENGKEALELVCNGLGNQRKHG 1020

Query: 1040 ASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPIIALTAHTTREEARRTIEAG 1099
            ASNTLPYD+ILMDCEMP+MDGYEATR+IRK+ERY+NTHIPIIALTAHTT EEA +TIEAG
Sbjct: 1021 ASNTLPYDYILMDCEMPIMDGYEATREIRKVERYYNTHIPIIALTAHTTGEEAGKTIEAG 1080

Query: 1100 MDVHLGKPLRKENLLEVIACIHSK 1124
            MDVHLGKPLRKE LLE I CIHSK
Sbjct: 1081 MDVHLGKPLRKEKLLEAITCIHSK 1103

BLAST of Spg003388 vs. NCBI nr
Match: XP_022965407.1 (histidine kinase CKI1 [Cucurbita maxima])

HSP 1 Score: 1737.2 bits (4498), Expect = 0.0e+00
Identity = 904/1127 (80.21%), Postives = 983/1127 (87.22%), Query Frame = 0

Query: 24   MEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQISYI 83
            ME+ISTSIQPIY STT+L K   SS NGTQIS  E+KSKIAP LFQGF+ IPYLTQISYI
Sbjct: 1    MERISTSIQPIYSSTTSLVKTFDSSLNGTQISSFELKSKIAPMLFQGFAIIPYLTQISYI 60

Query: 84   GMDGLFFSYYTDNNQTFAVYANSTFTARFY------PHPRREYTWLTQLVNSSTGETYGD 143
            G+DGLFFSYYTD NQTFAVY+NSTFTA+FY      P P RE++WLTQLVNSSTGE YGD
Sbjct: 61   GIDGLFFSYYTDKNQTFAVYSNSTFTAKFYPPPPPPPPPGREWSWLTQLVNSSTGELYGD 120

Query: 144  MAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSFGFSIK 203
            M +TLP  T+NTSW RDALN N+G AS+G KW SD E LLL T +VN  NGV+SFGFSIK
Sbjct: 121  MVETLPSVTTNTSWFRDALN-NQGSASVGKKWSSDQELLLLNTVRVNGRNGVLSFGFSIK 180

Query: 204  AFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSASFQFLNPNGDQIARVGN 263
            A +DLFFTSIERQGGRLYLA+TEGEILV GFQNI+MVL NGSASF+FL PNG++ ARV N
Sbjct: 181  ALIDLFFTSIERQGGRLYLASTEGEILVQGFQNIKMVLANGSASFRFLKPNGNETARVEN 240

Query: 264  ISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQL---------------------V 323
            ISC PRKE FDA+D FFNLLGT YMIYCSPLEILGVQL                     V
Sbjct: 241  ISCRPRKETFDAKDYFFNLLGTNYMIYCSPLEILGVQLLRTYESVHNYKNCLLLMFLFQV 300

Query: 324  YSLVLPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQ 383
            YSLVLPQKELASLV+KSSR+GLILLIL M+TTVISIFGFVFIVIRAA REM+LCAKLIQQ
Sbjct: 301  YSLVLPQKELASLVHKSSRMGLILLILTMSTTVISIFGFVFIVIRAANREMHLCAKLIQQ 360

Query: 384  MEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCT 443
            MEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEIC+NEA PGS+L+INLKQMDGCT
Sbjct: 361  MEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICHNEAAPGSDLNINLKQMDGCT 420

Query: 444  KDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGS 503
            KDLL ILNSILDTSKIEAGK QLEEEEF+L QLLEDVVDLYHPVGMKKGID+VLDPYDGS
Sbjct: 421  KDLLAILNSILDTSKIEAGKTQLEEEEFNLSQLLEDVVDLYHPVGMKKGIDIVLDPYDGS 480

Query: 504  VIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEIL 563
            +I FSQVKGDRGKLKQVLCNLLSN+VKFTSEG VTVRAWVKNLPDMQ+ +I S HNGEIL
Sbjct: 481  IINFSQVKGDRGKLKQVLCNLLSNSVKFTSEGHVTVRAWVKNLPDMQNKLIPSNHNGEIL 540

Query: 564  KHLSFLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVK 623
            KHLSFLLCK+T+  REQQ  DNGVHLN +C EF FEIDDTGKGIPKEKRKLVFENYVQVK
Sbjct: 541  KHLSFLLCKDTQTLREQQTADNGVHLNPNCYEFIFEIDDTGKGIPKEKRKLVFENYVQVK 600

Query: 624  ETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVT 683
            ETA GQ GTGLGLGIVQSLVRLMGGDI ILDKEIG KGTCFRFSVLL VS GNINSG  T
Sbjct: 601  ETAQGQ-GTGLGLGIVQSLVRLMGGDIEILDKEIGAKGTCFRFSVLLNVSEGNINSGNNT 660

Query: 684  CQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKV 743
            CQS   S+LTF+APSPS HSPR I+TT  KTETSRV+LLIRNDQRRM+CKKFME+LGV+V
Sbjct: 661  CQSLAASKLTFRAPSPSPHSPRPIQTTSLKTETSRVLLLIRNDQRRMICKKFMESLGVRV 720

Query: 744  LAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGA 803
            LAM QWEQL   LQKILEKQSHSRHS RGRSGNSS SD L+KS SG+S NG N +VSLGA
Sbjct: 721  LAMNQWEQLLFNLQKILEKQSHSRHSPRGRSGNSSSSDYLNKSASGNSSNGLNTNVSLGA 780

Query: 804  MKDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLL 863
            MK+ETNYLLSVFKKTSP+GGI+FILI+IDASAGPF EICNMV++FRTGLQ+AYCKVVWL+
Sbjct: 781  MKEETNYLLSVFKKTSPKGGITFILILIDASAGPFTEICNMVSNFRTGLQEAYCKVVWLV 840

Query: 864  ENQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIG 923
            ENQMS I+NHKGLDS++ E NDVVIS+PFHGSRLYEVI+LLPEFGGTLQSRESSRLYQ  
Sbjct: 841  ENQMSHIVNHKGLDSNILEFNDVVISRPFHGSRLYEVIRLLPEFGGTLQSRESSRLYQTD 900

Query: 924  SVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSK 983
            SV KDPSSSL +Y  KAKE NSP FR QIATRVQQ+ KSS+GTSPKNLSLNQIHS LGSK
Sbjct: 901  SVPKDPSSSLNEYCGKAKERNSPNFRDQIATRVQQETKSSTGTSPKNLSLNQIHSFLGSK 960

Query: 984  SRISPVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIE 1043
            +RISPV GQQS+HQE+          +KPL GKKILVAEDN+VLQ+LARLNLE+LGAT+E
Sbjct: 961  TRISPVSGQQSQHQEI----------QKPLGGKKILVAEDNVVLQRLARLNLEKLGATVE 1020

Query: 1044 ICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNT 1103
            ICENGE ALE VC+GLGNQRKHGASN LPYD+ILMDCEMPVMDGYEATRQIRK+ER +NT
Sbjct: 1021 ICENGEAALEFVCNGLGNQRKHGASNILPYDYILMDCEMPVMDGYEATRQIRKVERDYNT 1080

Query: 1104 HIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            HIPIIALTAH + EEARRTIEAGMDVHLGKPL+KE+LLE I CIHSK
Sbjct: 1081 HIPIIALTAHASGEEARRTIEAGMDVHLGKPLKKESLLEAIKCIHSK 1115

BLAST of Spg003388 vs. NCBI nr
Match: XP_038889343.1 (histidine kinase CKI1 [Benincasa hispida])

HSP 1 Score: 1728.0 bits (4474), Expect = 0.0e+00
Identity = 886/1107 (80.04%), Postives = 963/1107 (86.99%), Query Frame = 0

Query: 62   KIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREYT 121
            +IAP LFQGFS IPYLTQISYIGMDGLFFSYYTD NQTFAVYANSTFT +FYPHP REY+
Sbjct: 9    QIAPMLFQGFSIIPYLTQISYIGMDGLFFSYYTDKNQTFAVYANSTFTGKFYPHPPREYS 68

Query: 122  WLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTY 181
            WLTQLVNS+TGE YG+M +T PL TSNTSW RDALNSN+GCAS G +W SDHERL L T 
Sbjct: 69   WLTQLVNSTTGELYGNMTETFPLVTSNTSWFRDALNSNQGCASTGTRWSSDHERLFLNTV 128

Query: 182  KVNESNGVVSFGFSIKAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSAS 241
            +VN SN VVSFGFSIK F+DLFFTSIERQGGRLYLA TEGEILV G QNIRMVL NGSAS
Sbjct: 129  RVNGSNRVVSFGFSIKTFIDLFFTSIERQGGRLYLATTEGEILVIGSQNIRMVLANGSAS 188

Query: 242  FQFLNPNGDQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQL------ 301
            FQF NPNG +IAR+GNISCLPRK+DFD +DSFFNLLGTKY IYCSP+EI+GVQL      
Sbjct: 189  FQFFNPNGSEIARIGNISCLPRKKDFDPKDSFFNLLGTKYKIYCSPIEIMGVQLVSLIHT 248

Query: 302  ---------------------------------------VYSLVLPQKELASLVYKSSRV 361
                                                   VYSLVLPQKELASLV KSSR+
Sbjct: 249  VTITYILKILKYVNFIILLTMLGPNAYTLFLISGLFLFQVYSLVLPQKELASLVQKSSRM 308

Query: 362  GLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQ 421
            GLILLILIMTTTVISI GFVFIVIRA KREM+LCAKLIQQMEATQQAERKSMNKSVAF +
Sbjct: 309  GLILLILIMTTTVISISGFVFIVIRATKREMHLCAKLIQQMEATQQAERKSMNKSVAFAR 368

Query: 422  ASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGK 481
            ASHDI ASL GIIGL+EIC+NE  PGSELD+ LKQ+DGCTKDLLGILNSILDTSKIEAGK
Sbjct: 369  ASHDIHASLVGIIGLVEICHNEVAPGSELDMRLKQIDGCTKDLLGILNSILDTSKIEAGK 428

Query: 482  IQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQVKGDRGKLKQVLCN 541
            IQLEEEEFHLGQLLEDVVDL+HPVG+KKGID+VLDPYDGS+IKFSQVKGDRGKLKQVLCN
Sbjct: 429  IQLEEEEFHLGQLLEDVVDLFHPVGVKKGIDIVLDPYDGSIIKFSQVKGDRGKLKQVLCN 488

Query: 542  LLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSFLLCKNTERFREQQAT 601
            LLSNAVKFTSEGQVTVRAWVKNLP MQ+N+ISS HNGE+LKHLSFL+C +T  F+EQQA 
Sbjct: 489  LLSNAVKFTSEGQVTVRAWVKNLPAMQNNIISSNHNGEMLKHLSFLIC-STHTFQEQQAL 548

Query: 602  DNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALGQGGTGLGLGIVQSLV 661
            DNG++L  DCMEFTFEIDDTGKGIPKE R+LVFENYVQVKETAL QGG GLGLGIVQSLV
Sbjct: 549  DNGINLKPDCMEFTFEIDDTGKGIPKEMRRLVFENYVQVKETALRQGGAGLGLGIVQSLV 608

Query: 662  RLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSSPTSRLTFQAPSPSLHS 721
            RLMGGDIAILDKE  EKGTCFRF+VLLTV   N+NSG+ TCQSSPTSRLTF +PS SL S
Sbjct: 609  RLMGGDIAILDKETEEKGTCFRFNVLLTVLESNVNSGDNTCQSSPTSRLTFWSPSTSLRS 668

Query: 722  PRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQWEQLQVTLQKILEKQ 781
            PR IRTT SK ETSRVILLI+NDQRRM+CKKF+E+LG+KVLAMK+WEQL VTLQK LEKQ
Sbjct: 669  PRAIRTTSSKAETSRVILLIQNDQRRMICKKFLESLGIKVLAMKEWEQLLVTLQKTLEKQ 728

Query: 782  SHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNYLLSVFKKTSPRGG 841
            S S H+ RGRSGNSSPSDCLSKSTSGDS NG N+ VS+GAMKDE NYLLSVFKKT+ RGG
Sbjct: 729  SQSMHNLRGRSGNSSPSDCLSKSTSGDSSNGLNMHVSMGAMKDEKNYLLSVFKKTNLRGG 788

Query: 842  ISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQMSRIINHKGLDSSVFEL 901
             SFILIVIDASAGPF++IC+MVA+FR GLQ AYCKVVWLLENQMSRIIN KGL+S++F+ 
Sbjct: 789  NSFILIVIDASAGPFKDICDMVANFRRGLQGAYCKVVWLLENQMSRIINDKGLESNIFKS 848

Query: 902  NDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSKDPSSSLYQYHSKAKEG 961
            NDV IS+PFHGSRLYEVI+LLPEFGGTL++ ESSRLY  GSVSKDP+SS YQYHSKAKE 
Sbjct: 849  NDVFISRPFHGSRLYEVIRLLPEFGGTLETGESSRLYWSGSVSKDPNSSPYQYHSKAKEE 908

Query: 962  NSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRISPVVGQQSRHQELREEK 1021
            NSPI RGQI TRVQ++  SSSGTSPKNLS+NQIHSCLGSKSR SP+VGQ+S HQE+REEK
Sbjct: 909  NSPILRGQIRTRVQKETTSSSGTSPKNLSMNQIHSCLGSKSRSSPIVGQKSLHQEIREEK 968

Query: 1022 YENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICENGEEALELVCSGLGNQR 1081
            Y NL  EKPLSGKK LVAEDNLVLQKLARLNLERLGA IE CENG+EALELVC+GLGNQ 
Sbjct: 969  YRNLGVEKPLSGKKFLVAEDNLVLQKLARLNLERLGAAIETCENGKEALELVCNGLGNQW 1028

Query: 1082 KHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPIIALTAHTTREEARRTI 1124
            KHGASNTLPYD+ILMDCEMP+MDGYEATRQIRK+ERY+NTHIPIIALTAH T EEA +TI
Sbjct: 1029 KHGASNTLPYDYILMDCEMPIMDGYEATRQIRKVERYYNTHIPIIALTAHITEEEAGKTI 1088

BLAST of Spg003388 vs. NCBI nr
Match: XP_023537978.1 (histidine kinase CKI1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1719.9 bits (4453), Expect = 0.0e+00
Identity = 895/1128 (79.34%), Postives = 975/1128 (86.44%), Query Frame = 0

Query: 20   MSLEMEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQ 79
            MS+EME+ISTSIQPIY STT+L K   SS NG+QIS  E+KSKIAP LFQGF+ IPYLTQ
Sbjct: 1    MSIEMERISTSIQPIYSSTTSLVKTFDSSLNGSQISSFELKSKIAPMLFQGFAIIPYLTQ 60

Query: 80   ISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFY---PHPRREYTWLTQLVNSSTGETYG 139
            ISYIG+DGLFFSYYTD NQTFAVY+NSTFTA+FY   P P RE++W TQLVNSSTGE YG
Sbjct: 61   ISYIGIDGLFFSYYTDKNQTFAVYSNSTFTAKFYPPPPPPGREWSWRTQLVNSSTGELYG 120

Query: 140  DMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSFGFSI 199
            DM +TLP  T+ TSW RDALN N   AS+G KW SDHE LLL T +VN  NGV SFGFSI
Sbjct: 121  DMVETLPTVTTKTSWFRDALN-NEESASVGKKWSSDHELLLLNTVRVNGRNGVFSFGFSI 180

Query: 200  KAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSASFQFLNPNGDQIARVG 259
            KA +DLFFTSIERQGGRLYLA+TEGEILV GFQNI+MVL NGSASF+FL PN ++ ARV 
Sbjct: 181  KALIDLFFTSIERQGGRLYLASTEGEILVQGFQNIKMVLANGSASFRFLKPNDNETARVE 240

Query: 260  NISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQL--------------------- 319
            N+SC PRKE FDA+D F+NL GT YMIYCSPLEILGVQL                     
Sbjct: 241  NVSCRPRKEAFDAKDYFYNLFGTNYMIYCSPLEILGVQLLRTYESVHNYKNCLLLMFLFQ 300

Query: 320  VYSLVLPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQ 379
            VYSLVLPQKELASLV+KSSR+GLILLIL M+TTVISIFGFVFIVIRAA REM+LCAKLIQ
Sbjct: 301  VYSLVLPQKELASLVHKSSRMGLILLILTMSTTVISIFGFVFIVIRAANREMHLCAKLIQ 360

Query: 380  QMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGC 439
            QMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEIC+NEA PGS+L+INLKQMDGC
Sbjct: 361  QMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICHNEAAPGSDLNINLKQMDGC 420

Query: 440  TKDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDG 499
            TKDLL ILNSILDTSKIEAGK QLEEEEF+L QLLEDVVDLYHPVGMKKGID+VLDPYDG
Sbjct: 421  TKDLLAILNSILDTSKIEAGKTQLEEEEFNLSQLLEDVVDLYHPVGMKKGIDIVLDPYDG 480

Query: 500  SVIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEI 559
            S+I FSQVKGDRGKLKQVLCNLLSN+VKFTSEG VTVRAWVKNLPDMQ+ +I S HNGEI
Sbjct: 481  SIINFSQVKGDRGKLKQVLCNLLSNSVKFTSEGHVTVRAWVKNLPDMQNKLIPSNHNGEI 540

Query: 560  LKHLSFLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQV 619
            LKHLSFLLCK+T   REQQ  DNGVHLN +C EF FEIDDTGKGIPKEKRKLVFENYVQV
Sbjct: 541  LKHLSFLLCKDTPTLREQQTADNGVHLNPNCYEFIFEIDDTGKGIPKEKRKLVFENYVQV 600

Query: 620  KETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEV 679
            KETA GQ GTGLGLGIVQSLVRLMGGDI ILDKEIG KGTCFRFSVLL VS GNINSG  
Sbjct: 601  KETAQGQ-GTGLGLGIVQSLVRLMGGDIEILDKEIGAKGTCFRFSVLLNVSEGNINSGNN 660

Query: 680  TCQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVK 739
            TCQS    +LTF+APSPS  SPR I+TT  KTETSRV+LLIRNDQRRM+CKKFME+LGV+
Sbjct: 661  TCQSLAAPKLTFRAPSPSPRSPRPIQTTSLKTETSRVLLLIRNDQRRMICKKFMESLGVR 720

Query: 740  VLAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLG 799
            V+AM QWEQL  TLQKILEKQSHSRHS RGRSGNSS SD L+KS SG+S NG N + SLG
Sbjct: 721  VMAMNQWEQLLFTLQKILEKQSHSRHSPRGRSGNSSSSDYLNKSASGNSSNGLNTNASLG 780

Query: 800  AMKDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWL 859
            AMK+ETNYLLSVFKKTSP+GGI+FILIVIDA AGPF EICNMV++FRTGLQ+AYCKVVWL
Sbjct: 781  AMKEETNYLLSVFKKTSPKGGITFILIVIDAGAGPFTEICNMVSNFRTGLQEAYCKVVWL 840

Query: 860  LENQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQI 919
            +ENQMS I+NHKGLDS++ E NDVVIS+PFHGSRLYEVI+LLPEFGGTLQ RESSRLYQ 
Sbjct: 841  VENQMSHIVNHKGLDSNILEFNDVVISRPFHGSRLYEVIRLLPEFGGTLQGRESSRLYQS 900

Query: 920  GSVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGS 979
             SV KDPSSSL +Y  KAKE  SP FR QIATRVQQ+ KSS+GTSPKNLSLNQIHS LGS
Sbjct: 901  DSVPKDPSSSLNEYRGKAKERISPNFRDQIATRVQQETKSSTGTSPKNLSLNQIHSFLGS 960

Query: 980  KSRISPVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATI 1039
            K+RISPV GQQS+HQE+          +KPL GKKILVAEDN+VLQ+LARLNLE+LGAT+
Sbjct: 961  KTRISPVSGQQSQHQEI----------QKPLGGKKILVAEDNVVLQRLARLNLEKLGATV 1020

Query: 1040 EICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHN 1099
            EICENGE ALE VC+GLGNQRKHGASN LPYD+ILMDCEMPVMDGYEATRQIRK+ER +N
Sbjct: 1021 EICENGEAALEFVCNGLGNQRKHGASNILPYDYILMDCEMPVMDGYEATRQIRKVERDYN 1080

Query: 1100 THIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            THIPIIALTAH + EEARRTIEAGMDVHLGKPL+KE+LLE I CIHSK
Sbjct: 1081 THIPIIALTAHASGEEARRTIEAGMDVHLGKPLKKESLLEAIKCIHSK 1116

BLAST of Spg003388 vs. ExPASy Swiss-Prot
Match: O22267 (Histidine kinase CKI1 OS=Arabidopsis thaliana OX=3702 GN=CKI1 PE=1 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 6.1e-200
Identity = 443/1126 (39.34%), Postives = 643/1126 (57.10%), Query Frame = 0

Query: 36   VSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTD 95
            +ST  LA+++ S        F E++++IAP LF  +STI  ++Q+SYI  DGL FSY  +
Sbjct: 74   LSTIGLARVIDSYITNNDTGFTEIQTQIAPLLFVAYSTILQVSQVSYISRDGLMFSYIAE 133

Query: 96   NNQTFAVYANSTFTARFYPHPRREYTWLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDA 155
            +N + AV+ANS+  +      R +YTW TQ V+  TG   G+  ++  L  ++T W + A
Sbjct: 134  SNTSVAVFANSSSNS-----SRGDYTWYTQTVDQLTGRLNGNSTKSQSLDVTHTDWFQAA 193

Query: 156  LNSNRGCASIGPK-WGSDHERLLLYTYKVNESNGVVSFGFSIKAFVDLFFTSIERQGGRL 215
             ++N   A +G    G D+E L+     +    G+VS GF +K   ++   S+   G  L
Sbjct: 194  QSNNYTTAFVGTSLGGEDNETLIQSVVSLYSKKGLVSLGFPVKTLTEV-LNSLNLHGEEL 253

Query: 216  YLANTEGEILV-TGFQNIRMVLTNGSASFQFLNPNGDQIARVGN---ISCLPRKEDFDAR 275
            Y+   +G +LV  G  N    ++NGS  F           R  N     C+P  E+  + 
Sbjct: 254  YMWTKDGTVLVREGSLNDSFFISNGSICF----------GRESNSLWSQCIP--ENCSSS 313

Query: 276  DSFFNLLGTKYMIYCSPLEILGVQLVYSLVLPQKELASLVYKSSRVGLILLILIMTTTVI 335
                 +   +Y  +CS +E+ GV L Y+L+ P K  A+ +   +      LI++M   + 
Sbjct: 314  GYEVEIKRLRYQAFCSVIEVSGVPLRYTLMFPNKGGATRIKHQAEKAKYQLIVVM---IF 373

Query: 336  SIFG----FVFIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQASHDIRASLA 395
              FG    FV+ +++A +REM++ A LI QMEATQQAERKSMNKS AF  ASHDIR +LA
Sbjct: 374  LGFGWPVWFVWFMMQATRREMHMRATLINQMEATQQAERKSMNKSQAFANASHDIRGALA 433

Query: 396  GIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGKIQLEEEEFHL 455
            G+ GLI+IC +   PGS++D  L Q++ C KDL+ +LNS+LD SKIE+GK+QL EE+F+L
Sbjct: 434  GMKGLIDICRDGVKPGSDVDTTLNQVNVCAKDLVALLNSVLDMSKIESGKMQLVEEDFNL 493

Query: 456  GQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQVKGDRGKLKQVLCNLLSNAVKFTS 515
             +LLEDV+D YHPV MKKG+D+VLDP+DGSV KFS V+GD G+LKQ+L NL+SNAVKFT 
Sbjct: 494  SKLLEDVIDFYHPVAMKKGVDVVLDPHDGSVFKFSNVRGDSGRLKQILNNLVSNAVKFTV 553

Query: 516  EGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSFLLCKNTERFREQQA-TDNGVHLNSD 575
            +G + VRAW +  P   S+++ + +   + K +  + CKN E     +    N +  N++
Sbjct: 554  DGHIAVRAWAQR-PGSNSSVVLASYPKGVSKFVKSMFCKNKEESSTYETEISNSIRNNAN 613

Query: 576  CMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALGQGGTGLGLGIVQSLVRLMGGDIAI 635
             MEF FE+DDTGKGIP E RK VFENYVQV+ETA G  GTGLGLGIVQSLVRLMGG+I I
Sbjct: 614  TMEFVFEVDDTGKGIPMEMRKSVFENYVQVRETAQGHQGTGLGLGIVQSLVRLMGGEIRI 673

Query: 636  LDKEIGEKGTCFRFSVLLT----------------------VSVGNINSGEVTCQSSPTS 695
             DK +GEKGTCF+F+VLLT                      VS  N+    +T  +S   
Sbjct: 674  TDKAMGEKGTCFQFNVLLTTLESPPVSDMKVRQEIEAGGDYVSTPNLG---LTINTSLGG 733

Query: 696  RLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQWE 755
             +  +  SP  ++     ++  K E SRV+LL++N++RR V +K+++ LG+KV  +++WE
Sbjct: 734  SMNIRNLSPRFNN---CLSSSPKQEGSRVVLLLKNEERRRVTEKYIKNLGIKVTVVEKWE 793

Query: 756  QLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNY 815
             L   L+++      S  SS GR      ++C     S  S   P I +           
Sbjct: 794  HLSYALERLF---GFSPQSSMGR------AECSLSCPS--SRELPFIGMD---------- 853

Query: 816  LLSVFKKTSPRGGISF---ILIVIDASAGPFREICNMVASFRTGLQDAY-CKVVWLLENQ 875
             +    +   R  ISF   +L+VIDA  GPF E+C++V  FR GL     CKVVWL E+ 
Sbjct: 854  GIDSRSQLPKRRSISFSAVVLLVIDAKTGPFFELCDIVKQFRRGLPHGISCKVVWLNES- 913

Query: 876  MSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVS 935
                       + V E  D+  S+P HGSRL EV+K+LPEFGGT+     + L +   + 
Sbjct: 914  ----------STRVSERGDISCSRPLHGSRLMEVLKMLPEFGGTVLKEPPTELQRESLLR 973

Query: 936  KDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRI 995
                +     H   +EG S +F  ++  R+     S S T  K++   +           
Sbjct: 974  HSFVAERSPKHKVQEEGPSSMFNKKLGKRIMASTDSESETRVKSVRTGR----------- 1033

Query: 996  SPVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGAT-IEIC 1055
             P+   +       E++    + ++ L GK++LV +DN + +K+A   L+++G + +E C
Sbjct: 1034 KPIGNPED------EQETSKPSDDEFLRGKRVLVVDDNFISRKVATGKLKKMGVSEVEQC 1093

Query: 1056 ENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHI 1115
            ++G+EAL LV  GL  + + G+ + LP+D+I MDC+MP MDGYEATR+IRK+E+ +    
Sbjct: 1094 DSGKEALRLVTEGLTQREEQGSVDKLPFDYIFMDCQMPEMDGYEATREIRKVEKSYGVRT 1120

Query: 1116 PIIALTAHTT-REEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            PIIA++ H    EEAR TI+AGMD  L K L +  L  VI  I SK
Sbjct: 1154 PIIAVSGHDPGSEEARETIQAGMDAFLDKSLNQ--LANVIREIESK 1120

BLAST of Spg003388 vs. ExPASy Swiss-Prot
Match: A2YA15 (Probable histidine kinase 2 OS=Oryza sativa subsp. indica OX=39946 GN=HK2 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.6e-120
Identity = 342/1109 (30.84%), Postives = 520/1109 (46.89%), Query Frame = 0

Query: 20   MSLEMEKISTSIQPIYVSTTNLAKILGS-SFNGTQISFLEVKSKIAPTLFQGFSTIPYLT 79
            M   +  I+ +++P+  +  ++  I  +    G   SF    S + P LF  FS  P   
Sbjct: 56   MDSALMHIAGNMRPLLEANRSVFTIANTLHVQGNMASF----SHVGPKLFLAFSMQPLQA 115

Query: 80   QISYIGMDGLFFSYYT---DNNQTFAVYANSTFTARFYPHPRREYTWLTQLVNSSTGETY 139
            QISY  +DG  F+YY     + +  A++A            R   TW TQ V+ +TG   
Sbjct: 116  QISYAAVDGAAFAYYRAGGGDGEARAMFA------------RPNGTWFTQAVDPATGRPV 175

Query: 140  GDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSFGFS 199
            G+     P      +  R  L+   G AS+   W     R+L  +  V    G VS   +
Sbjct: 176  GNATAAAPHQQLPPNVTRLLLDGGGGGASLADGWARPGVRMLFLSAPVGGGGGAVSAAVA 235

Query: 200  IKAFVDLFFTSIE--RQGGRLY-LANTEGEILVTGFQN---IRMVLTNGSASFQFLNPNG 259
            +   V      +   R  G  Y +A   G             R +L +G+A+        
Sbjct: 236  VDDVVLRGAAGLRQLRDLGMYYAVAGNGGATAAPPAPEPAAYRSLLGDGAAA-------- 295

Query: 260  DQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLVLPQKELASL 319
            +++A   ++ C     D   +     +   KY   C+  +I GVQ+ + +VL +  +  +
Sbjct: 296  EEMALFSSVKCTASAIDAPPKLDVHGVKSDKYRFACTNFDISGVQMGFRVVLRKSAMVGV 355

Query: 320  VYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEATQQAERKSMN 379
              +     + +         ++       + RA  RE  L A L +  +A +QAERKSMN
Sbjct: 356  FRRGGVTMVAVACAAAAAATVACVLMARALRRAVAREAALGADLARHRDALRQAERKSMN 415

Query: 380  KSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDT 439
            KS AF  ASHDIR++LA + GL+E+   EA P   +  NL QM+ CT  LL ILNSILDT
Sbjct: 416  KSNAFASASHDIRSALAAVAGLVEVSRPEANP--NIVDNLNQMELCTNKLLDILNSILDT 475

Query: 440  SKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQVKGDRGK 499
            +K+E+GK+QLEE EF++  +LE+ VD+ + VG+ KGI+++ DP D SV+K   + GD  +
Sbjct: 476  TKVESGKVQLEEVEFNMADVLEESVDMANVVGITKGIEVIWDPCDFSVMKCDNIIGDSKR 535

Query: 500  LKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSFLLCKNTER 559
             KQ+L NLL NA+KFT EG V +RAW        S    S+     L++  F      + 
Sbjct: 536  FKQILDNLLGNAMKFTQEGHVILRAWANRPIARGSIGAPSRFAYRSLENNFFSFFFGAKE 595

Query: 560  FREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALGQGGTGLGL 619
             R  Q + N +  + + +EF FE+ DTG GIPKEKR+ VFENYVQVKE   G GGTGLGL
Sbjct: 596  DRVSQNSFNPLQNDPNSVEFYFEVVDTGIGIPKEKRESVFENYVQVKE---GHGGTGLGL 655

Query: 620  GIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSSPTSRLTFQA 679
            GIVQS VRLMGG+I+I +KE GE+GTCF F+VLL  S       ++    S  S L  +A
Sbjct: 656  GIVQSFVRLMGGEISIKEKEPGERGTCFGFNVLLKTSGNQAAEEDIEEGPSTVSELDIRA 715

Query: 680  PSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQWEQLQVTL 739
                     + R T    +    IL +  D+ R V + +ME++G+KV  +   E +  TL
Sbjct: 716  --------SVFRETNC-FKGWHCILFVHGDETRRVLQAWMESIGMKVWMVPGVESISSTL 775

Query: 740  QKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNYLLSVFK 799
            +K                  SS  DC          +   +   L       N +     
Sbjct: 776  EK----------------ARSSRDDC---DVDRCFSSKEMVSQVLPTTLRNNNIMARNLG 835

Query: 800  KTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQMSRIINHKGL 859
            +  P G    +L+++D S G    I      F        CK V L + + S    +K  
Sbjct: 836  EHHPLG----MLLIVDVSNGQLENIQRQARDFTQMRSQVPCKFVCLTDLRTS----YKDF 895

Query: 860  DSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSKDPSSSLYQY 919
                    D+++ KP HGSRLY ++        TL+  +SS +++   V  +       Y
Sbjct: 896  RRFEEMSCDLILRKPVHGSRLYSLLM-------TLRDVQSSPMHRSSLVGHE------NY 955

Query: 920  HSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRISPVVGQQSRH 979
             ++ ++  + +   ++  R+ Q  K+                                  
Sbjct: 956  VTRHQDSANIVALAEVG-RLDQGLKTEE-------------------------------- 1015

Query: 980  QELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICENGEEALELVC 1039
                         ++PL G  +L+ ED LVLQ + R  L +LGA +E+  +G +A+++  
Sbjct: 1016 -------------DRPLDGMHVLLVEDTLVLQTIQRKMLNQLGAIVELAGDGAKAVDMFR 1040

Query: 1040 SGLGNQR-KHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPIIALTAHTT 1099
              +         S  LPYD I MDC+MP MDGYEATR+IR+ E  +    PIIALTAH+ 
Sbjct: 1076 DAIERASVSEEHSVPLPYDVIFMDCQMPRMDGYEATRRIREEESRYGIRTPIIALTAHSM 1040

Query: 1100 REEARRTIEAGMDVHLGKPLRKENLLEVI 1118
             ++ ++ I+ GMD+H+ KP+ +  ++E +
Sbjct: 1136 EDDLQKAIDVGMDLHMTKPIERRRIVEAV 1040

BLAST of Spg003388 vs. ExPASy Swiss-Prot
Match: Q5SML4 (Probable histidine kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=HK2 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.6e-120
Identity = 342/1109 (30.84%), Postives = 520/1109 (46.89%), Query Frame = 0

Query: 20   MSLEMEKISTSIQPIYVSTTNLAKILGS-SFNGTQISFLEVKSKIAPTLFQGFSTIPYLT 79
            M   +  I+ +++P+  +  ++  I  +    G   SF    S + P LF  FS  P   
Sbjct: 56   MDSALMHIAGNMRPLLEANRSVFTIANTLHVQGNMASF----SHVGPKLFLAFSMQPLQA 115

Query: 80   QISYIGMDGLFFSYYT---DNNQTFAVYANSTFTARFYPHPRREYTWLTQLVNSSTGETY 139
            QISY  +DG  F+YY     + +  A++A            R   TW TQ V+ +TG   
Sbjct: 116  QISYAAVDGAAFAYYRAGGGDGEARAMFA------------RPNGTWFTQAVDPATGRPV 175

Query: 140  GDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSFGFS 199
            G+     P      +  R  L+   G AS+   W     R+L  +  V    G VS   +
Sbjct: 176  GNATAAAPHQQLPPNVTRLLLDGGGGGASLADGWARPGVRMLFLSAPVGGGGGAVSAAVA 235

Query: 200  IKAFVDLFFTSIE--RQGGRLY-LANTEGEILVTGFQN---IRMVLTNGSASFQFLNPNG 259
            +   V      +   R  G  Y +A   G             R +L +G+A+        
Sbjct: 236  VDDVVLRGAAGLRQLRDLGMYYAVAGNGGATAAPPAPEPAAYRSLLGDGAAA-------- 295

Query: 260  DQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLVLPQKELASL 319
            +++A   ++ C     D   +     +   KY   C+  +I GVQ+ + +VL +  +  +
Sbjct: 296  EEMALFSSVKCTASAIDAPPKLDVHGVKSDKYRFACTNFDISGVQMGFRVVLRKSAMVGV 355

Query: 320  VYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEATQQAERKSMN 379
              +     + +         ++       + RA  RE  L A L +  +A +QAERKSMN
Sbjct: 356  FRRGGVTMVAVACAAAAAATVACVLMARALRRAVAREAALGADLARHRDALRQAERKSMN 415

Query: 380  KSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDT 439
            KS AF  ASHDIR++LA + GL+E+   EA P   +  NL QM+ CT  LL ILNSILDT
Sbjct: 416  KSNAFASASHDIRSALAAVAGLVEVSRPEANP--NIVDNLNQMELCTNKLLDILNSILDT 475

Query: 440  SKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQVKGDRGK 499
            +K+E+GK+QLEE EF++  +LE+ VD+ + VG+ KGI+++ DP D SV+K   + GD  +
Sbjct: 476  TKVESGKVQLEEVEFNMADVLEESVDMANVVGITKGIEVIWDPCDFSVMKCDNIIGDSKR 535

Query: 500  LKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSFLLCKNTER 559
             KQ+L NLL NA+KFT EG V +RAW        S    S+     L++  F      + 
Sbjct: 536  FKQILDNLLGNAMKFTQEGHVILRAWANRPIARGSIGAPSRFAYRSLENNFFSFFFGAKE 595

Query: 560  FREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALGQGGTGLGL 619
             R  Q + N +  + + +EF FE+ DTG GIPKEKR+ VFENYVQVKE   G GGTGLGL
Sbjct: 596  DRVSQNSFNPLQNDPNSVEFYFEVVDTGIGIPKEKRESVFENYVQVKE---GHGGTGLGL 655

Query: 620  GIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSSPTSRLTFQA 679
            GIVQS VRLMGG+I+I +KE GE+GTCF F+VLL  S       ++    S  S L  +A
Sbjct: 656  GIVQSFVRLMGGEISIKEKEPGERGTCFGFNVLLKTSGNQAAEEDIEEGPSTVSELDIRA 715

Query: 680  PSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQWEQLQVTL 739
                     + R T    +    IL +  D+ R V + +ME++G+KV  +   E +  TL
Sbjct: 716  --------SVFRETNC-FKGWHCILFVHGDETRRVLQAWMESIGMKVWMVPGVESISSTL 775

Query: 740  QKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNYLLSVFK 799
            +K                  SS  DC          +   +   L       N +     
Sbjct: 776  EK----------------ARSSRDDC---DVDRCFSSKEMVSQVLPTTLRNNNIMARNLG 835

Query: 800  KTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQMSRIINHKGL 859
            +  P G    +L+++D S G    I      F        CK V L + + S    +K  
Sbjct: 836  EHHPLG----MLLIVDVSNGQLENIQRQARDFTQMRSQVPCKFVCLTDLRTS----YKDF 895

Query: 860  DSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSKDPSSSLYQY 919
                    D+++ KP HGSRLY ++        TL+  +SS +++   V  +       Y
Sbjct: 896  RRFEEMSCDLILRKPVHGSRLYSLLM-------TLRDVQSSPMHRSSLVGHE------NY 955

Query: 920  HSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRISPVVGQQSRH 979
             ++ ++  + +   ++  R+ Q  K+                                  
Sbjct: 956  VTRHQDSANIVALAEVG-RLDQGLKTEE-------------------------------- 1015

Query: 980  QELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICENGEEALELVC 1039
                         ++PL G  +L+ ED LVLQ + R  L +LGA +E+  +G +A+++  
Sbjct: 1016 -------------DRPLDGMHVLLVEDTLVLQTIQRKMLNQLGAIVELAGDGAKAVDMFR 1040

Query: 1040 SGLGNQR-KHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPIIALTAHTT 1099
              +         S  LPYD I MDC+MP MDGYEATR+IR+ E  +    PIIALTAH+ 
Sbjct: 1076 DAIERASVSEEHSVPLPYDVIFMDCQMPRMDGYEATRRIREEESRYGIRTPIIALTAHSM 1040

Query: 1100 REEARRTIEAGMDVHLGKPLRKENLLEVI 1118
             ++ ++ I+ GMD+H+ KP+ +  ++E +
Sbjct: 1136 EDDLQKAIDVGMDLHMTKPIERRRIVEAV 1040

BLAST of Spg003388 vs. ExPASy Swiss-Prot
Match: Q9SXL4 (Histidine kinase 1 OS=Arabidopsis thaliana OX=3702 GN=AHK1 PE=1 SV=2)

HSP 1 Score: 265.0 bits (676), Expect = 3.9e-69
Identity = 293/1166 (25.13%), Postives = 500/1166 (42.88%), Query Frame = 0

Query: 15   NATSEMSLEMEKISTSIQPIYVSTT---NLAKILGSSFNGTQISFLEVKSKIAPTL---- 74
            N TSE++    K+S  +   Y   T    L ++  +  + T   F   K+  A T+    
Sbjct: 135  NTTSELTTAQVKLSEYVIKKYDKPTTQEELVEMYQAMKDVTWALFASAKALNAITINYRN 194

Query: 75   --FQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREYTWLTQ 134
               Q F   P  +   YI  D   +S      +  + + N +       H      W  Q
Sbjct: 195  GFVQAFHRDPASSSTFYIFSDLKNYSISGTGPEDVSGWNNKSI------HGNMSAIWYQQ 254

Query: 135  LVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNE 194
             ++  TGE  G   +  P          D L +  G + +     S H  +  Y      
Sbjct: 255  QLDPVTGENLGKPLKIPP----------DDLINIAGISQVPDGEASWHVTVSKYMDSPLL 314

Query: 195  SNGVVSFGFSIKAFV-------------DLFFTSIERQGGRLYLANTEGEILVTGFQNIR 254
            S  +  F  S K+ V              L    +E  GG +YL + EG +L T      
Sbjct: 315  SAALPVFDASNKSIVAVVGVTTALYSVGQLMRDLVEVHGGHIYLTSQEGYLLATSTDGPL 374

Query: 255  MVLTNGSASFQFLNPNGDQIARVGN---ISCLPRKEDFDARDSFFNLLGTKYMIYCSPLE 314
            +  T+          + + + + G          K           L   +Y I    L 
Sbjct: 375  LKNTSNGPQLMKATDSEEWVIKSGAQWLEKTYGSKRPHVVHAENVKLGDQRYYIDSFYLN 434

Query: 315  ILGVQLVYSLVLPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVI--RAAKREM 374
            +  + +V  +++P+K +   V    R    L+ILI  +  I   G V I+I      +EM
Sbjct: 435  LKRLPIVGVVIIPRKFIMGKV--DERAFKTLIILISASVCIFFIGCVCILILTNGVSKEM 494

Query: 375  YLCAKLIQQMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDI 434
             L A+LI+Q++A ++AE  S  KS      SH++R  +A +IGL++I  ++    +E   
Sbjct: 495  KLRAELIRQLDARRRAEASSNYKSQFLANMSHELRTPMAAVIGLLDILISDDCLSNEQYA 554

Query: 435  NLKQMDGCTKDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGID 494
             + Q+  C+  LL +LN+ILD SK+E+GK+ LEE EF LG+ LE +VD++    +   ++
Sbjct: 555  TVTQIRKCSTALLRLLNNILDLSKVESGKLVLEEAEFDLGRELEGLVDMFSVQCINHNVE 614

Query: 495  LVLDPYDGSVIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMI 554
             VLD  D      + V+GD  +L Q+  NL+SN++KFT+ G + +R W +N+  +   M 
Sbjct: 615  TVLDLSDDMP---ALVRGDSARLVQIFANLISNSIKFTTTGHIILRGWCENINSLHDEMS 674

Query: 555  SSKHNGEILKHLSFLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKL 614
             S    +  K  + +  K  +     Q +      N++ M   FE+DDTG GI   K   
Sbjct: 675  VSV---DRRKPWAPMKTKQVQHRNHLQKSCK----NANKMVLWFEVDDTGCGIDPSKWDS 734

Query: 615  VFENYVQV-KETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVS 674
            VFE++ Q    T    GGTGLGL IV++LV  MGG+I ++ K     GT  R  ++L+  
Sbjct: 735  VFESFEQADPSTTRTHGGTGLGLCIVRNLVNKMGGEIKVVQK--NGLGTLMRLYLILS-- 794

Query: 675  VGNINSGEVTCQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCK 734
                           T     Q   P      ++           V+L +     RM+  
Sbjct: 795  ---------------TPDTVDQNIQPDFSKYGLV-----------VMLSMYGSTARMITS 854

Query: 735  KFMETLGVKVLAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGN 794
            K++   G+  +    W +L   ++ +LE                      S+  S DS +
Sbjct: 855  KWLRKHGIATVEASDWNELTQIIRDLLETG--------------------SRDNSFDSQH 914

Query: 795  GPNIDVSLGAMKDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQ 854
              NI   L A       L ++ +  +P   I   + V+D +   ++E  N +  F     
Sbjct: 915  --NISDPLRA------ELSNIVEIKNPVFVIVVDIGVLDLTTNIWKEQLNYLDRFSN--- 974

Query: 855  DAYCKVVWLLENQMSRII----NHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPE--- 914
                K  WLL++  S  +      KG        + ++++KP + +++ ++++ + +   
Sbjct: 975  --KAKFAWLLKHDTSNTVKTELRRKG--------HVMMVNKPLYKAKMIQILEAVIKNRK 1034

Query: 915  --FGGTLQSRESSRLYQIGSVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSS 974
                  L++R +        +  DP+    Q+ + + + +S    G+   +V +  K S+
Sbjct: 1035 RGLCNDLRNRGNGSDESHDCLEIDPT----QFDTCSSDDSSET-SGE--KQVDKSVKPST 1094

Query: 975  GTSP--KNLSLNQIHSCLGSKSRISPVVGQQSRHQELREEKYENL----NGEKPLSGKKI 1034
              SP  KN  ++   S     S  + +  +    ++ ++  Y  +      +K L G +I
Sbjct: 1095 LHSPVLKNYLIDATTS--NDDSTSASMTQKNPEEEDWKDRLYSGIALDGKNQKSLEGIRI 1154

Query: 1035 LVAEDNLVLQKLARLNLERLGATIEICENGEEALELV--------------------CSG 1094
            L+AED  VLQ++A + LE++GAT+    +G++A++ +                     + 
Sbjct: 1155 LLAEDTPVLQRVATIMLEKMGATVTAVWDGQQAVDSLNYKSINAQAPTEEHKSFEEETAN 1192

Query: 1095 LGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPIIALTAHTTREE 1118
                R+    N+ PYD ILMDC+MP MDGYEAT+ IR+ E     HIPI+ALTAH    +
Sbjct: 1215 KVTTRETSLRNSSPYDLILMDCQMPKMDGYEATKAIRRAEIGTELHIPIVALTAHAMSSD 1192

BLAST of Spg003388 vs. ExPASy Swiss-Prot
Match: A1A698 (Probable histidine kinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=HK4 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.1e-34
Identity = 194/805 (24.10%), Postives = 317/805 (39.38%), Query Frame = 0

Query: 355  QQMEA-TQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMD 414
            ++MEA  ++AE   + KS      SH+IR  + G++G++++  +  +  ++ D   +   
Sbjct: 367  RKMEALKKRAEAADIAKSQFLATVSHEIRTPMNGVLGMLDMLLDTELKSTQRDY-AQTAQ 426

Query: 415  GCTKDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPY 474
             C K L+ ++N +LD +KIEAGKI LE   F L  +L+DV+ L+     +KGI+L +  Y
Sbjct: 427  VCGKALISLINEVLDRAKIEAGKIDLESVPFDLRSILDDVISLFSSKSREKGIELAV--Y 486

Query: 475  DGSVIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNM----ISS 534
                +    + GD G+ +Q++ NL+ N++KFT  G + V+  + +  ++ +      + +
Sbjct: 487  VSERVP-EILLGDPGRFRQIITNLVGNSIKFTERGHIFVQVHLADHSNLATEAKIEPVVN 546

Query: 535  KHNGEILKHLSFLLC---------------KNTERFR---EQQATDNGVHLNSDCMEFTF 594
              NG   + ++                    N E F+     +  +     +SD +    
Sbjct: 547  GMNGHKDEAIAIPTSGSHNTLSGFEAADSRNNWENFKLLLSYEKNEMPYESDSDKVTLVV 606

Query: 595  EIDDTGKGIPKEKRKLVFENYVQV-KETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEI 654
             ++DTG GIP   +  VF  ++Q    T+   GGTG+GL I + LV +MGG I  + + +
Sbjct: 607  SVEDTGIGIPLHAQGRVFTPFMQADSSTSRNYGGTGIGLSISKCLVEIMGGQINFVSRPL 666

Query: 655  GEKGTCFRFSVLLTVSVGNINSGEVTCQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETS 714
               G+ F F+ +L     N  S   T    P    +F+  S  L   R +R T       
Sbjct: 667  --VGSTFTFTAVLRRCDKNAISDSKTVALHPLPS-SFKGLSALLVDKRPVRAT------- 726

Query: 715  RVILLIRNDQRRMVCKKFMETLGVKVLAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNS 774
                         V K  ++ LG+         ++  T+       S    SS    G  
Sbjct: 727  -------------VTKYHLQRLGIT-------SEVVGTIDPTFGVLSGRNGSSLTSIGKK 786

Query: 775  SPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNYLLSVFKKTSPRGGISFILIVIDASAGP 834
             P   L +S S     GP +DVSL A   E         K S R                
Sbjct: 787  QPCMLLIESDSW----GPQMDVSLHARLQE--------MKQSDR---------------- 846

Query: 835  FREICNMVASFRTGLQDAYCKVVWLLENQMSRIINHKGLDSSVFELNDVVISKPFHGSRL 894
                                                                        
Sbjct: 847  ------------------------------------------------------------ 906

Query: 895  YEVIKLLPEFGGTLQSRESSRLYQIGSVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQ 954
               I +LP+    L + ES ++ +I +V      S+     KA    + +F+    T+  
Sbjct: 907  ---IHVLPKV-FLLSAAESDKVKKIHAV-----DSVIPKPLKASALAACLFQALGITQPS 966

Query: 955  QDKKSSSGTSPKNLSLNQIHSCLGSKSRISPVVGQQSRHQELREEKYENLNGEKPLSGKK 1014
             +K+  SG+         +H   GS S                      L+G   L GK 
Sbjct: 967  HEKRDDSGS---------LHGRDGSGS----------------------LHG--LLLGKN 995

Query: 1015 ILVAEDNLVLQKLARLNLERLGATIEICENGEEALELVCSGLGNQRKHGASNTLPYDFIL 1074
            ILV +DN V  ++A   L++ GA +E  E+G++AL L+      Q  H       +D  L
Sbjct: 1027 ILVVDDNKVNLRVAAGTLKKYGAKVECVESGKDALSLL------QVPH------KFDLCL 995

Query: 1075 MDCEMPVMDGYEATRQIRKMERYHNT------------------HIPIIALTAHTTREEA 1118
            MD +MP MDG+EATRQIR ME   N                   H+PI+A+TA   +   
Sbjct: 1087 MDIQMPEMDGFEATRQIRAMEGKANEQADDSESGSEIAAKTAKWHLPILAMTADVIQATH 995

BLAST of Spg003388 vs. ExPASy TrEMBL
Match: A0A5A7TXY4 (Histidine kinase CKI1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001400 PE=4 SV=1)

HSP 1 Score: 1803.1 bits (4669), Expect = 0.0e+00
Identity = 919/1123 (81.83%), Postives = 1008/1123 (89.76%), Query Frame = 0

Query: 1    MIKHIHSHLLVNNYNATSEMSLEMEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVK 60
            MIKHI SH L NNYNATS+M  E+E+IS+SIQPIYVSTTN AK+L S FNGTQ+SF E+ 
Sbjct: 36   MIKHIQSHYLFNNYNATSQMPHEIEEISSSIQPIYVSTTNFAKLLDSYFNGTQVSFFELN 95

Query: 61   SKIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREY 120
            SKIAP LFQGFS IPYLTQISYIG DGLFFSYYTD NQTFAVYANSTFTA+FYP+PRREY
Sbjct: 96   SKIAPILFQGFSIIPYLTQISYIGTDGLFFSYYTDKNQTFAVYANSTFTAKFYPNPRREY 155

Query: 121  TWLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYT 180
            +WLTQ  NS+TGE YG+M + LPL TSNTSW RDALNSN+GCASIG KW S+HERL L T
Sbjct: 156  SWLTQSANSTTGELYGNMTEILPLVTSNTSWFRDALNSNQGCASIGTKWSSNHERLFLNT 215

Query: 181  YKVNESNGVVSFGFSIKAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSA 240
             +V  SNGVVSFGFS K F+DL FTS+ERQGGRLYLA  EGEILV G Q+I+MVL NGSA
Sbjct: 216  VRVTGSNGVVSFGFSFKTFIDLLFTSMERQGGRLYLATNEGEILVLGSQDIKMVLANGSA 275

Query: 241  SFQFLNPNGDQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLV 300
            +FQFLNPNG +IAR+GNISC  RKED D +DSFFNLLGT Y+IYC PLEILGVQLVYSLV
Sbjct: 276  TFQFLNPNGGEIARLGNISCQARKEDSDPKDSFFNLLGTDYIIYCYPLEILGVQLVYSLV 335

Query: 301  LPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEAT 360
            LPQKELASLVYKSSR+GLILLILIM  T+I++  FVFIVIRA KREM+LCAKLIQQMEAT
Sbjct: 336  LPQKELASLVYKSSRMGLILLILIMAITIITVLVFVFIVIRATKREMHLCAKLIQQMEAT 395

Query: 361  QQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLL 420
            QQAERKSMNKSVAFT+ASHDIRASLAGIIGLIEIC+NE+ PGSELDI+LKQMDGCTKDLL
Sbjct: 396  QQAERKSMNKSVAFTRASHDIRASLAGIIGLIEICHNESTPGSELDISLKQMDGCTKDLL 455

Query: 421  GILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKF 480
            GILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVG+KKGID+VLDPYDGS+IKF
Sbjct: 456  GILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGVKKGIDVVLDPYDGSIIKF 515

Query: 481  SQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLS 540
            SQVKGDRGKLKQ+LCNLLSNAVKFTSEGQVTVRAWVKNLP MQ+NMISS HN EILKH S
Sbjct: 516  SQVKGDRGKLKQILCNLLSNAVKFTSEGQVTVRAWVKNLPTMQNNMISSNHNDEILKHFS 575

Query: 541  FLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETAL 600
            FL+C NT  F+EQQA DNGV+LN  CMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETA 
Sbjct: 576  FLVC-NTNTFQEQQAMDNGVNLNPACMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETAF 635

Query: 601  GQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSS 660
            GQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLL V   N+++G+ T Q S
Sbjct: 636  GQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLIVLEDNVHTGDDTRQPS 695

Query: 661  PTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMK 720
            PTSRLTF APS SLHSPR IRTT SKTETSRVILLI+NDQRR++CKKF+E+LGVKVLAMK
Sbjct: 696  PTSRLTFWAPSTSLHSPRAIRTTSSKTETSRVILLIQNDQRRLICKKFLESLGVKVLAMK 755

Query: 721  QWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDE 780
            +WEQL  TLQKIL+KQSHS+H+SRGRSGNSSPSD LSKSTS DSGNG N+ VS GA KDE
Sbjct: 756  EWEQLLTTLQKILDKQSHSKHNSRGRSGNSSPSDYLSKSTSDDSGNGLNMHVSSGARKDE 815

Query: 781  TNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQM 840
            TNY LSVFKKT+ RGG SFILIVIDASAGPF+EICNMVA+FR GLQ AYCKVVWLLE QM
Sbjct: 816  TNYFLSVFKKTNLRGGNSFILIVIDASAGPFKEICNMVANFRRGLQGAYCKVVWLLEKQM 875

Query: 841  SRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSK 900
            SRI N KG+DS++ +LNDV IS+PFHGSRLYEVI+LLPEFGGTL++ ESS  Y  G+VSK
Sbjct: 876  SRISNDKGIDSNIDKLNDVFISRPFHGSRLYEVIRLLPEFGGTLETGESSTSYWSGNVSK 935

Query: 901  DPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRIS 960
            DPSSS YQ  SK KEGNSPIFRG I TRVQ++  S S TSPKNLS+NQIHSC+GSK+R S
Sbjct: 936  DPSSSAYQCQSKCKEGNSPIFRGHIETRVQKETTSRSWTSPKNLSMNQIHSCIGSKTRSS 995

Query: 961  PVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICEN 1020
            P+V Q+S HQE+REEKY++ +GEKPL GKK+LVAEDNL+LQKLARLNLERLGAT EICEN
Sbjct: 996  PIVEQKSLHQEIREEKYKHSSGEKPLCGKKVLVAEDNLLLQKLARLNLERLGATTEICEN 1055

Query: 1021 GEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPI 1080
            G+EALELVC+GLGNQRKHGASNTLPYD+ILMDCEMP+MDGYEATR+IRK+ERY+NTHIPI
Sbjct: 1056 GKEALELVCNGLGNQRKHGASNTLPYDYILMDCEMPIMDGYEATREIRKVERYYNTHIPI 1115

Query: 1081 IALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            IALTAHTT EEA +TIEAGMDVHLGKPLRKE LLE I CIHSK
Sbjct: 1116 IALTAHTTGEEAGKTIEAGMDVHLGKPLRKEKLLEAITCIHSK 1157

BLAST of Spg003388 vs. ExPASy TrEMBL
Match: A0A6J1HK93 (histidine kinase CKI1 OS=Cucurbita maxima OX=3661 GN=LOC111465321 PE=4 SV=1)

HSP 1 Score: 1737.2 bits (4498), Expect = 0.0e+00
Identity = 904/1127 (80.21%), Postives = 983/1127 (87.22%), Query Frame = 0

Query: 24   MEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQISYI 83
            ME+ISTSIQPIY STT+L K   SS NGTQIS  E+KSKIAP LFQGF+ IPYLTQISYI
Sbjct: 1    MERISTSIQPIYSSTTSLVKTFDSSLNGTQISSFELKSKIAPMLFQGFAIIPYLTQISYI 60

Query: 84   GMDGLFFSYYTDNNQTFAVYANSTFTARFY------PHPRREYTWLTQLVNSSTGETYGD 143
            G+DGLFFSYYTD NQTFAVY+NSTFTA+FY      P P RE++WLTQLVNSSTGE YGD
Sbjct: 61   GIDGLFFSYYTDKNQTFAVYSNSTFTAKFYPPPPPPPPPGREWSWLTQLVNSSTGELYGD 120

Query: 144  MAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSFGFSIK 203
            M +TLP  T+NTSW RDALN N+G AS+G KW SD E LLL T +VN  NGV+SFGFSIK
Sbjct: 121  MVETLPSVTTNTSWFRDALN-NQGSASVGKKWSSDQELLLLNTVRVNGRNGVLSFGFSIK 180

Query: 204  AFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSASFQFLNPNGDQIARVGN 263
            A +DLFFTSIERQGGRLYLA+TEGEILV GFQNI+MVL NGSASF+FL PNG++ ARV N
Sbjct: 181  ALIDLFFTSIERQGGRLYLASTEGEILVQGFQNIKMVLANGSASFRFLKPNGNETARVEN 240

Query: 264  ISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQL---------------------V 323
            ISC PRKE FDA+D FFNLLGT YMIYCSPLEILGVQL                     V
Sbjct: 241  ISCRPRKETFDAKDYFFNLLGTNYMIYCSPLEILGVQLLRTYESVHNYKNCLLLMFLFQV 300

Query: 324  YSLVLPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQ 383
            YSLVLPQKELASLV+KSSR+GLILLIL M+TTVISIFGFVFIVIRAA REM+LCAKLIQQ
Sbjct: 301  YSLVLPQKELASLVHKSSRMGLILLILTMSTTVISIFGFVFIVIRAANREMHLCAKLIQQ 360

Query: 384  MEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCT 443
            MEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEIC+NEA PGS+L+INLKQMDGCT
Sbjct: 361  MEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICHNEAAPGSDLNINLKQMDGCT 420

Query: 444  KDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGS 503
            KDLL ILNSILDTSKIEAGK QLEEEEF+L QLLEDVVDLYHPVGMKKGID+VLDPYDGS
Sbjct: 421  KDLLAILNSILDTSKIEAGKTQLEEEEFNLSQLLEDVVDLYHPVGMKKGIDIVLDPYDGS 480

Query: 504  VIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEIL 563
            +I FSQVKGDRGKLKQVLCNLLSN+VKFTSEG VTVRAWVKNLPDMQ+ +I S HNGEIL
Sbjct: 481  IINFSQVKGDRGKLKQVLCNLLSNSVKFTSEGHVTVRAWVKNLPDMQNKLIPSNHNGEIL 540

Query: 564  KHLSFLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVK 623
            KHLSFLLCK+T+  REQQ  DNGVHLN +C EF FEIDDTGKGIPKEKRKLVFENYVQVK
Sbjct: 541  KHLSFLLCKDTQTLREQQTADNGVHLNPNCYEFIFEIDDTGKGIPKEKRKLVFENYVQVK 600

Query: 624  ETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVT 683
            ETA GQ GTGLGLGIVQSLVRLMGGDI ILDKEIG KGTCFRFSVLL VS GNINSG  T
Sbjct: 601  ETAQGQ-GTGLGLGIVQSLVRLMGGDIEILDKEIGAKGTCFRFSVLLNVSEGNINSGNNT 660

Query: 684  CQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKV 743
            CQS   S+LTF+APSPS HSPR I+TT  KTETSRV+LLIRNDQRRM+CKKFME+LGV+V
Sbjct: 661  CQSLAASKLTFRAPSPSPHSPRPIQTTSLKTETSRVLLLIRNDQRRMICKKFMESLGVRV 720

Query: 744  LAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGA 803
            LAM QWEQL   LQKILEKQSHSRHS RGRSGNSS SD L+KS SG+S NG N +VSLGA
Sbjct: 721  LAMNQWEQLLFNLQKILEKQSHSRHSPRGRSGNSSSSDYLNKSASGNSSNGLNTNVSLGA 780

Query: 804  MKDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLL 863
            MK+ETNYLLSVFKKTSP+GGI+FILI+IDASAGPF EICNMV++FRTGLQ+AYCKVVWL+
Sbjct: 781  MKEETNYLLSVFKKTSPKGGITFILILIDASAGPFTEICNMVSNFRTGLQEAYCKVVWLV 840

Query: 864  ENQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIG 923
            ENQMS I+NHKGLDS++ E NDVVIS+PFHGSRLYEVI+LLPEFGGTLQSRESSRLYQ  
Sbjct: 841  ENQMSHIVNHKGLDSNILEFNDVVISRPFHGSRLYEVIRLLPEFGGTLQSRESSRLYQTD 900

Query: 924  SVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSK 983
            SV KDPSSSL +Y  KAKE NSP FR QIATRVQQ+ KSS+GTSPKNLSLNQIHS LGSK
Sbjct: 901  SVPKDPSSSLNEYCGKAKERNSPNFRDQIATRVQQETKSSTGTSPKNLSLNQIHSFLGSK 960

Query: 984  SRISPVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIE 1043
            +RISPV GQQS+HQE+          +KPL GKKILVAEDN+VLQ+LARLNLE+LGAT+E
Sbjct: 961  TRISPVSGQQSQHQEI----------QKPLGGKKILVAEDNVVLQRLARLNLEKLGATVE 1020

Query: 1044 ICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNT 1103
            ICENGE ALE VC+GLGNQRKHGASN LPYD+ILMDCEMPVMDGYEATRQIRK+ER +NT
Sbjct: 1021 ICENGEAALEFVCNGLGNQRKHGASNILPYDYILMDCEMPVMDGYEATRQIRKVERDYNT 1080

Query: 1104 HIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            HIPIIALTAH + EEARRTIEAGMDVHLGKPL+KE+LLE I CIHSK
Sbjct: 1081 HIPIIALTAHASGEEARRTIEAGMDVHLGKPLKKESLLEAIKCIHSK 1115

BLAST of Spg003388 vs. ExPASy TrEMBL
Match: A0A0A0LM39 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034540 PE=4 SV=1)

HSP 1 Score: 1725.3 bits (4467), Expect = 0.0e+00
Identity = 875/1062 (82.39%), Postives = 961/1062 (90.49%), Query Frame = 0

Query: 62   KIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREYT 121
            +IAP LFQGFS IPYLTQISYIGMDGLFFSYYTD NQTFAVYANSTFTA+FYPHPRREY+
Sbjct: 4    QIAPMLFQGFSIIPYLTQISYIGMDGLFFSYYTDKNQTFAVYANSTFTAKFYPHPRREYS 63

Query: 122  WLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTY 181
            WLTQL NS+TGE YG+M + LPL TSNTSW RDALNSN+GCASIG KW S+HERL L T 
Sbjct: 64   WLTQLANSTTGELYGNMTEILPLVTSNTSWFRDALNSNQGCASIGTKWSSNHERLFLNTV 123

Query: 182  KVNESNGVVSFGFSIKAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSAS 241
            +VN S GVVSFGFS K F+DLFFTSIERQGGRLYLA+ EGEILV G Q+I+MVL NGSA+
Sbjct: 124  RVNGSKGVVSFGFSFKTFIDLFFTSIERQGGRLYLASNEGEILVLGSQDIKMVLVNGSAT 183

Query: 242  FQFLNPNGDQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLVL 301
            FQFLNPNG +IAR+GNISC  RKEDFD +DSFFNLLGT Y IYC P+EILGVQLVYSLVL
Sbjct: 184  FQFLNPNGGEIARLGNISCQARKEDFDPKDSFFNLLGTNYNIYCYPVEILGVQLVYSLVL 243

Query: 302  PQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEATQ 361
            PQKELASL++KSSR+GLILLILIM  T+I++F FVFIVIR  KREM+LCAKLIQQMEATQ
Sbjct: 244  PQKELASLIFKSSRMGLILLILIMAITIITVFIFVFIVIRGTKREMHLCAKLIQQMEATQ 303

Query: 362  QAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLG 421
            QAERKSMNKSVAFT+ASHDIRASLAGIIGLIEIC+NE+ PGSELDI+LKQMDGCTKDLLG
Sbjct: 304  QAERKSMNKSVAFTRASHDIRASLAGIIGLIEICHNESTPGSELDISLKQMDGCTKDLLG 363

Query: 422  ILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFS 481
            ILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVG+KKGIDLVLDPYDGS+IKFS
Sbjct: 364  ILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGVKKGIDLVLDPYDGSIIKFS 423

Query: 482  QVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSF 541
            QVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLP MQ+NMISS HN EILK+ SF
Sbjct: 424  QVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPTMQNNMISSNHNDEILKYFSF 483

Query: 542  LLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALG 601
            L+C NT  F+EQQA DNGV+LN  CMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETA G
Sbjct: 484  LVC-NTSTFQEQQAMDNGVNLNPGCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETAFG 543

Query: 602  QGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSSP 661
            QGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLL V   N+N+G+ T Q SP
Sbjct: 544  QGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLLVLEDNVNTGDDTRQPSP 603

Query: 662  TSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQ 721
            TS+LTF AP+ SLHSPR IRTT SKTETSRVILLI+NDQRR++CKKF+E+LGVKVLAMKQ
Sbjct: 604  TSKLTFWAPTTSLHSPRAIRTTSSKTETSRVILLIQNDQRRLICKKFLESLGVKVLAMKQ 663

Query: 722  WEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDET 781
            WEQL  TLQKIL+KQSHS H+SRGRSG SSPSDCLSKSTSGDS NG N+ +S GA KDET
Sbjct: 664  WEQLLTTLQKILDKQSHSMHNSRGRSGTSSPSDCLSKSTSGDSRNGLNMHISSGARKDET 723

Query: 782  NYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQMS 841
            NY LSVFKKT+ RGG SFILIVIDA AGPF+EICNMVA+FR  LQ ++CKVVWLLENQMS
Sbjct: 724  NYFLSVFKKTNLRGGNSFILIVIDARAGPFKEICNMVANFRRRLQGSHCKVVWLLENQMS 783

Query: 842  RIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSKD 901
            RI N KG+DS++++ NDV IS+PFHGSRLYEVI+LLPEFGGTL++ ESS  Y  G+VSKD
Sbjct: 784  RISNDKGIDSNIYKSNDVFISRPFHGSRLYEVIRLLPEFGGTLETGESSTSYWSGNVSKD 843

Query: 902  PSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRISP 961
             SSS YQ  SK+KEGNSPIFRG I TRVQ++  SS+GTSPKNLS NQIHSC+GSK+R SP
Sbjct: 844  LSSSPYQCQSKSKEGNSPIFRGHIETRVQKETTSSNGTSPKNLSTNQIHSCIGSKTRSSP 903

Query: 962  VVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICENG 1021
            +V Q+S HQE+REEKY++ +GEKPLSGKK+LVAEDNLVLQKLARLNLERLGAT EICENG
Sbjct: 904  IVEQKSLHQEIREEKYKHSSGEKPLSGKKVLVAEDNLVLQKLARLNLERLGATTEICENG 963

Query: 1022 EEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPII 1081
            +EALELVC+GLGNQRKHGASNTLPYD+ILMDCEMP+MDGYEATR+IRK+ERY+NTHIPII
Sbjct: 964  KEALELVCNGLGNQRKHGASNTLPYDYILMDCEMPIMDGYEATREIRKVERYYNTHIPII 1023

Query: 1082 ALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            ALTAHTT EEA +TIEAGMDVHLGKPLRKE LLE I CIHSK
Sbjct: 1024 ALTAHTTGEEAGKTIEAGMDVHLGKPLRKEKLLEAITCIHSK 1064

BLAST of Spg003388 vs. ExPASy TrEMBL
Match: A0A6J1FCT0 (histidine kinase CKI1 OS=Cucurbita moschata OX=3662 GN=LOC111444446 PE=4 SV=1)

HSP 1 Score: 1718.0 bits (4448), Expect = 0.0e+00
Identity = 895/1129 (79.27%), Postives = 974/1129 (86.27%), Query Frame = 0

Query: 20   MSLEMEKISTSIQPIYVSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQ 79
            MS+EME+ISTSIQPIY STT+L K   SS NGTQIS  E+KSKIAP LFQGF+ IPYLTQ
Sbjct: 1    MSIEMERISTSIQPIYSSTTSLVKTFDSSLNGTQISSFELKSKIAPMLFQGFAIIPYLTQ 60

Query: 80   ISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFY-------PHPRREYTWLTQLVNSSTG 139
            ISYIG+DGLFFSYYTD NQTFAVY+NSTF A+FY       P P RE++WLTQLVNSSTG
Sbjct: 61   ISYIGIDGLFFSYYTDKNQTFAVYSNSTFNAKFYPPPPPPPPPPGREWSWLTQLVNSSTG 120

Query: 140  ETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNESNGVVSF 199
            E YGDM +TLP  T+ TSW RDALN N   AS+G KW SDHE LLL T +VN  NGV SF
Sbjct: 121  ELYGDMVETLPTVTTKTSWFRDALN-NEESASVGKKWSSDHELLLLNTVRVNGRNGVFSF 180

Query: 200  GFSIKAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSASFQFLNPNGDQI 259
            GFSIKA +DLFFTSIERQGGRLYLA+TEGEILV GFQNI+MVL NG ASF+FL PNG++ 
Sbjct: 181  GFSIKALIDLFFTSIERQGGRLYLASTEGEILVQGFQNIKMVLANGLASFRFLKPNGNET 240

Query: 260  ARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQL----------------- 319
            ARV N+SC PRKE FDA+D FFNL GT YMIYCSPLEILGVQ                  
Sbjct: 241  ARVENVSCRPRKEAFDAKDYFFNLFGTNYMIYCSPLEILGVQTYESVHSYKNCLLLVFSF 300

Query: 320  -VYSLVLPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLI 379
             VYSLVLPQKELASLV+KSSR+GLILLIL M+TTVISIFGFVFIVIRAA REM+LCAKLI
Sbjct: 301  QVYSLVLPQKELASLVHKSSRMGLILLILTMSTTVISIFGFVFIVIRAANREMHLCAKLI 360

Query: 380  QQMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDG 439
            QQMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEIC+NEA PGS+L+INLKQMDG
Sbjct: 361  QQMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICHNEASPGSDLNINLKQMDG 420

Query: 440  CTKDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYD 499
            CTKDLL ILNSILDTSKIEAGK QLEEEEF+L QLLEDVVDLYHPVGMKKGID+VLDPYD
Sbjct: 421  CTKDLLAILNSILDTSKIEAGKTQLEEEEFNLSQLLEDVVDLYHPVGMKKGIDIVLDPYD 480

Query: 500  GSVIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGE 559
            GS+I FSQVKGDRGKLKQVLCNLLSN+VKFTSEG VTVRAWVKNLPDMQ+ +I S HNGE
Sbjct: 481  GSIINFSQVKGDRGKLKQVLCNLLSNSVKFTSEGHVTVRAWVKNLPDMQNKLIPSNHNGE 540

Query: 560  ILKHLSFLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQ 619
            ILKHLSFLLCK+T   REQQ  DNGVHLN +C EF FEIDDTGKGIPKEKRKLVFENYVQ
Sbjct: 541  ILKHLSFLLCKDTPTLREQQTADNGVHLNPNCYEFIFEIDDTGKGIPKEKRKLVFENYVQ 600

Query: 620  VKETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGE 679
            VKETA GQ GTGLGLGIVQSLVRLMGGDI ILDKEIG KGTCFRFSVLL VS GNINSG 
Sbjct: 601  VKETAQGQ-GTGLGLGIVQSLVRLMGGDIEILDKEIGAKGTCFRFSVLLNVSEGNINSGN 660

Query: 680  VTCQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGV 739
             +CQS   S+LTF+APSPS  SPR I+TT  KTETSRV+LLIRNDQRRM+CKKF+E+LGV
Sbjct: 661  NSCQSLAASKLTFRAPSPSPRSPRPIQTTSLKTETSRVLLLIRNDQRRMICKKFLESLGV 720

Query: 740  KVLAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSL 799
            +V+AM QWEQL  TLQKILEKQSHSRHS RGRSGNSS  D L+KS SG+S NG N +VSL
Sbjct: 721  RVMAMNQWEQLLFTLQKILEKQSHSRHSPRGRSGNSSSGDYLNKSASGNSSNGLNTNVSL 780

Query: 800  GAMKDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVW 859
            GAMKDETNYLLSVFKKTSP+GGI+FILIVIDA AGPF EICNMV++FRTGLQ+AYCKVVW
Sbjct: 781  GAMKDETNYLLSVFKKTSPKGGITFILIVIDAGAGPFTEICNMVSNFRTGLQEAYCKVVW 840

Query: 860  LLENQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQ 919
            L+ENQMS I+NHKGLDS++ E NDVVIS+PFHGSRLYEVI+LLPEFGGTL SRESSRLYQ
Sbjct: 841  LVENQMSHIVNHKGLDSNILEFNDVVISRPFHGSRLYEVIRLLPEFGGTLHSRESSRLYQ 900

Query: 920  IGSVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLG 979
              SV KDPSSSL +Y  KAKE  SP FR QIATRVQQ+ KSS+GTSPKNLSLNQIHS LG
Sbjct: 901  TDSVPKDPSSSLNEYRGKAKERISPNFRDQIATRVQQETKSSTGTSPKNLSLNQIHSFLG 960

Query: 980  SKSRISPVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGAT 1039
            SK+RISPV GQQS+HQE+          +KPL GKKILVAEDN+VLQ+LARLNLE+LGAT
Sbjct: 961  SKTRISPVSGQQSQHQEI----------QKPLGGKKILVAEDNVVLQRLARLNLEKLGAT 1020

Query: 1040 IEICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYH 1099
            +EICENGE ALE VC+GLGNQRKHGASN LPYD+ILMDCEMPVMDGYEATRQIRK+ER +
Sbjct: 1021 VEICENGEAALEFVCNGLGNQRKHGASNILPYDYILMDCEMPVMDGYEATRQIRKVERDY 1080

Query: 1100 NTHIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            NT IPIIALTAH + EEARRTIEAGMDVHLGKPL+KE+LLE I CIHSK
Sbjct: 1081 NTRIPIIALTAHASGEEARRTIEAGMDVHLGKPLKKESLLEAIKCIHSK 1117

BLAST of Spg003388 vs. ExPASy TrEMBL
Match: A0A1S3BWD0 (LOW QUALITY PROTEIN: histidine kinase CKI1-like OS=Cucumis melo OX=3656 GN=LOC103494157 PE=4 SV=1)

HSP 1 Score: 1701.0 bits (4404), Expect = 0.0e+00
Identity = 868/1062 (81.73%), Postives = 948/1062 (89.27%), Query Frame = 0

Query: 62   KIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREYT 121
            +IAP LFQGFS IPYLTQISYIGMDGLFFSYYTD NQTFAVYANSTFTA+FYP+PRREY+
Sbjct: 4    QIAPILFQGFSIIPYLTQISYIGMDGLFFSYYTDKNQTFAVYANSTFTAKFYPNPRREYS 63

Query: 122  WLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTY 181
            WLTQ  NS+TGE YG+M + LPL TSNTSW RDALNSN+GCASIG KW S+HERL L T 
Sbjct: 64   WLTQSANSTTGELYGNMTEILPLVTSNTSWFRDALNSNQGCASIGTKWSSNHERLFLNTV 123

Query: 182  KVNESNGVVSFGFSIKAFVDLFFTSIERQGGRLYLANTEGEILVTGFQNIRMVLTNGSAS 241
            +VN SNGVVSFGFS K F+DL FTSIERQGGRLYLA  EGEILV G Q+I+MVL NGSA+
Sbjct: 124  RVNGSNGVVSFGFSFKTFIDLLFTSIERQGGRLYLATNEGEILVRGSQDIKMVLANGSAT 183

Query: 242  FQFLNPNGDQIARVGNISCLPRKEDFDARDSFFNLLGTKYMIYCSPLEILGVQLVYSLVL 301
            FQFLNPNG +IAR+GNISC  RKED D +DSFFNLLGT Y+IYC PLEILGVQLVYSLVL
Sbjct: 184  FQFLNPNGGEIARLGNISCRARKEDSDPKDSFFNLLGTDYIIYCYPLEILGVQLVYSLVL 243

Query: 302  PQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVIRAAKREMYLCAKLIQQMEATQ 361
            PQKELASLVYKSSR+GLILLILIM  T+I++  FVFIVIRA KREM+LCAKLIQQMEATQ
Sbjct: 244  PQKELASLVYKSSRMGLILLILIMAITIITVLVFVFIVIRATKREMHLCAKLIQQMEATQ 303

Query: 362  QAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLG 421
            QAERKSMNKSVAFT+ASHDIRASLAGIIGLIEIC+NE+ PGSELDI+LKQMDGCTKDLLG
Sbjct: 304  QAERKSMNKSVAFTRASHDIRASLAGIIGLIEICHNESTPGSELDISLKQMDGCTKDLLG 363

Query: 422  ILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFS 481
            ILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVG+KKGID+VLDPYDGS+IKFS
Sbjct: 364  ILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGVKKGIDVVLDPYDGSIIKFS 423

Query: 482  QVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSF 541
            QVKGDRGKLKQ+LCNLLSNAVKFTSEGQVTVRAWVKNLP MQ+NMISS HN EILKH SF
Sbjct: 424  QVKGDRGKLKQILCNLLSNAVKFTSEGQVTVRAWVKNLPTMQNNMISSNHNDEILKHFSF 483

Query: 542  LLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALG 601
            L+C NT  F+EQQA DNGV+LN  CMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETA G
Sbjct: 484  LVC-NTNTFQEQQAMDNGVNLNPACMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETAFG 543

Query: 602  QGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTCQSSP 661
            QGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLL V   N+++G+ T Q SP
Sbjct: 544  QGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLIVLEDNVHTGDDTRQPSP 603

Query: 662  TSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQ 721
            TSRLTF APS SLHSPR IRTT SKTETSRVILLI+NDQRR++CKKF+E+LGVKVLAMK+
Sbjct: 604  TSRLTFWAPSTSLHSPRAIRTTSSKTETSRVILLIQNDQRRLICKKFLESLGVKVLAMKE 663

Query: 722  WEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDET 781
            WEQL  TLQKIL+KQSHS+H+SRGRSGNSSPSDCLSKSTS DSGNG N+ VS GA KDET
Sbjct: 664  WEQLLTTLQKILDKQSHSKHNSRGRSGNSSPSDCLSKSTSDDSGNGLNMHVSSGARKDET 723

Query: 782  NYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLENQMS 841
            NY LSVFKKT+ RGG SFILIVIDASAGPF+EICNMVA+FR GLQ AYCKVVWLLE QMS
Sbjct: 724  NYFLSVFKKTNLRGGNSFILIVIDASAGPFKEICNMVANFRRGLQGAYCKVVWLLEKQMS 783

Query: 842  RIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVSKD 901
            RI N KG+DS++ +LNDV IS+PFHGSRLYEVI+LLPEFGGTL++ ESS  Y  G+VSKD
Sbjct: 784  RISNDKGIDSNIDKLNDVFISRPFHGSRLYEVIRLLPEFGGTLETGESSTSYWSGNVSKD 843

Query: 902  PSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRISP 961
            PSSS YQ  SK KEGNSPIFRG I TRVQ++  S S TSPKNLS+NQIHSC+GSK+R SP
Sbjct: 844  PSSSAYQCQSKCKEGNSPIFRGHIETRVQKETTSRSWTSPKNLSMNQIHSCIGSKTRSSP 903

Query: 962  VVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGATIEICENG 1021
            +V Q+S HQEL +        +     KK+LVAEDNLVLQKLARLNLERLGAT EICENG
Sbjct: 904  IVEQKSLHQELEKRNINTRVAKNLYVAKKVLVAEDNLVLQKLARLNLERLGATTEICENG 963

Query: 1022 EEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPII 1081
            +EALELVC+GLGNQRK+GASNTLPYD+ILMDCEMP+MDGYEATR+IRK+ERY+NTHIPII
Sbjct: 964  KEALELVCNGLGNQRKYGASNTLPYDYILMDCEMPIMDGYEATREIRKVERYYNTHIPII 1023

Query: 1082 ALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            ALTAHTT EEA +TIEAGMDVHLGKPLRKE LLE I CIHSK
Sbjct: 1024 ALTAHTTGEEAGKTIEAGMDVHLGKPLRKEKLLEAITCIHSK 1064

BLAST of Spg003388 vs. TAIR 10
Match: AT2G47430.1 (Signal transduction histidine kinase )

HSP 1 Score: 699.5 bits (1804), Expect = 4.3e-201
Identity = 443/1126 (39.34%), Postives = 643/1126 (57.10%), Query Frame = 0

Query: 36   VSTTNLAKILGSSFNGTQISFLEVKSKIAPTLFQGFSTIPYLTQISYIGMDGLFFSYYTD 95
            +ST  LA+++ S        F E++++IAP LF  +STI  ++Q+SYI  DGL FSY  +
Sbjct: 74   LSTIGLARVIDSYITNNDTGFTEIQTQIAPLLFVAYSTILQVSQVSYISRDGLMFSYIAE 133

Query: 96   NNQTFAVYANSTFTARFYPHPRREYTWLTQLVNSSTGETYGDMAQTLPLATSNTSWVRDA 155
            +N + AV+ANS+  +      R +YTW TQ V+  TG   G+  ++  L  ++T W + A
Sbjct: 134  SNTSVAVFANSSSNS-----SRGDYTWYTQTVDQLTGRLNGNSTKSQSLDVTHTDWFQAA 193

Query: 156  LNSNRGCASIGPK-WGSDHERLLLYTYKVNESNGVVSFGFSIKAFVDLFFTSIERQGGRL 215
             ++N   A +G    G D+E L+     +    G+VS GF +K   ++   S+   G  L
Sbjct: 194  QSNNYTTAFVGTSLGGEDNETLIQSVVSLYSKKGLVSLGFPVKTLTEV-LNSLNLHGEEL 253

Query: 216  YLANTEGEILV-TGFQNIRMVLTNGSASFQFLNPNGDQIARVGN---ISCLPRKEDFDAR 275
            Y+   +G +LV  G  N    ++NGS  F           R  N     C+P  E+  + 
Sbjct: 254  YMWTKDGTVLVREGSLNDSFFISNGSICF----------GRESNSLWSQCIP--ENCSSS 313

Query: 276  DSFFNLLGTKYMIYCSPLEILGVQLVYSLVLPQKELASLVYKSSRVGLILLILIMTTTVI 335
                 +   +Y  +CS +E+ GV L Y+L+ P K  A+ +   +      LI++M   + 
Sbjct: 314  GYEVEIKRLRYQAFCSVIEVSGVPLRYTLMFPNKGGATRIKHQAEKAKYQLIVVM---IF 373

Query: 336  SIFG----FVFIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQASHDIRASLA 395
              FG    FV+ +++A +REM++ A LI QMEATQQAERKSMNKS AF  ASHDIR +LA
Sbjct: 374  LGFGWPVWFVWFMMQATRREMHMRATLINQMEATQQAERKSMNKSQAFANASHDIRGALA 433

Query: 396  GIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGKIQLEEEEFHL 455
            G+ GLI+IC +   PGS++D  L Q++ C KDL+ +LNS+LD SKIE+GK+QL EE+F+L
Sbjct: 434  GMKGLIDICRDGVKPGSDVDTTLNQVNVCAKDLVALLNSVLDMSKIESGKMQLVEEDFNL 493

Query: 456  GQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQVKGDRGKLKQVLCNLLSNAVKFTS 515
             +LLEDV+D YHPV MKKG+D+VLDP+DGSV KFS V+GD G+LKQ+L NL+SNAVKFT 
Sbjct: 494  SKLLEDVIDFYHPVAMKKGVDVVLDPHDGSVFKFSNVRGDSGRLKQILNNLVSNAVKFTV 553

Query: 516  EGQVTVRAWVKNLPDMQSNMISSKHNGEILKHLSFLLCKNTERFREQQA-TDNGVHLNSD 575
            +G + VRAW +  P   S+++ + +   + K +  + CKN E     +    N +  N++
Sbjct: 554  DGHIAVRAWAQR-PGSNSSVVLASYPKGVSKFVKSMFCKNKEESSTYETEISNSIRNNAN 613

Query: 576  CMEFTFEIDDTGKGIPKEKRKLVFENYVQVKETALGQGGTGLGLGIVQSLVRLMGGDIAI 635
             MEF FE+DDTGKGIP E RK VFENYVQV+ETA G  GTGLGLGIVQSLVRLMGG+I I
Sbjct: 614  TMEFVFEVDDTGKGIPMEMRKSVFENYVQVRETAQGHQGTGLGLGIVQSLVRLMGGEIRI 673

Query: 636  LDKEIGEKGTCFRFSVLLT----------------------VSVGNINSGEVTCQSSPTS 695
             DK +GEKGTCF+F+VLLT                      VS  N+    +T  +S   
Sbjct: 674  TDKAMGEKGTCFQFNVLLTTLESPPVSDMKVRQEIEAGGDYVSTPNLG---LTINTSLGG 733

Query: 696  RLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVLAMKQWE 755
             +  +  SP  ++     ++  K E SRV+LL++N++RR V +K+++ LG+KV  +++WE
Sbjct: 734  SMNIRNLSPRFNN---CLSSSPKQEGSRVVLLLKNEERRRVTEKYIKNLGIKVTVVEKWE 793

Query: 756  QLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAMKDETNY 815
             L   L+++      S  SS GR      ++C     S  S   P I +           
Sbjct: 794  HLSYALERLF---GFSPQSSMGR------AECSLSCPS--SRELPFIGMD---------- 853

Query: 816  LLSVFKKTSPRGGISF---ILIVIDASAGPFREICNMVASFRTGLQDAY-CKVVWLLENQ 875
             +    +   R  ISF   +L+VIDA  GPF E+C++V  FR GL     CKVVWL E+ 
Sbjct: 854  GIDSRSQLPKRRSISFSAVVLLVIDAKTGPFFELCDIVKQFRRGLPHGISCKVVWLNES- 913

Query: 876  MSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGSVS 935
                       + V E  D+  S+P HGSRL EV+K+LPEFGGT+     + L +   + 
Sbjct: 914  ----------STRVSERGDISCSRPLHGSRLMEVLKMLPEFGGTVLKEPPTELQRESLLR 973

Query: 936  KDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSPKNLSLNQIHSCLGSKSRI 995
                +     H   +EG S +F  ++  R+     S S T  K++   +           
Sbjct: 974  HSFVAERSPKHKVQEEGPSSMFNKKLGKRIMASTDSESETRVKSVRTGR----------- 1033

Query: 996  SPVVGQQSRHQELREEKYENLNGEKPLSGKKILVAEDNLVLQKLARLNLERLGAT-IEIC 1055
             P+   +       E++    + ++ L GK++LV +DN + +K+A   L+++G + +E C
Sbjct: 1034 KPIGNPED------EQETSKPSDDEFLRGKRVLVVDDNFISRKVATGKLKKMGVSEVEQC 1093

Query: 1056 ENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHI 1115
            ++G+EAL LV  GL  + + G+ + LP+D+I MDC+MP MDGYEATR+IRK+E+ +    
Sbjct: 1094 DSGKEALRLVTEGLTQREEQGSVDKLPFDYIFMDCQMPEMDGYEATREIRKVEKSYGVRT 1120

Query: 1116 PIIALTAHTT-REEARRTIEAGMDVHLGKPLRKENLLEVIACIHSK 1124
            PIIA++ H    EEAR TI+AGMD  L K L +  L  VI  I SK
Sbjct: 1154 PIIAVSGHDPGSEEARETIQAGMDAFLDKSLNQ--LANVIREIESK 1120

BLAST of Spg003388 vs. TAIR 10
Match: AT2G17820.1 (histidine kinase 1 )

HSP 1 Score: 265.0 bits (676), Expect = 2.7e-70
Identity = 293/1166 (25.13%), Postives = 500/1166 (42.88%), Query Frame = 0

Query: 15   NATSEMSLEMEKISTSIQPIYVSTT---NLAKILGSSFNGTQISFLEVKSKIAPTL---- 74
            N TSE++    K+S  +   Y   T    L ++  +  + T   F   K+  A T+    
Sbjct: 135  NTTSELTTAQVKLSEYVIKKYDKPTTQEELVEMYQAMKDVTWALFASAKALNAITINYRN 194

Query: 75   --FQGFSTIPYLTQISYIGMDGLFFSYYTDNNQTFAVYANSTFTARFYPHPRREYTWLTQ 134
               Q F   P  +   YI  D   +S      +  + + N +       H      W  Q
Sbjct: 195  GFVQAFHRDPASSSTFYIFSDLKNYSISGTGPEDVSGWNNKSI------HGNMSAIWYQQ 254

Query: 135  LVNSSTGETYGDMAQTLPLATSNTSWVRDALNSNRGCASIGPKWGSDHERLLLYTYKVNE 194
             ++  TGE  G   +  P          D L +  G + +     S H  +  Y      
Sbjct: 255  QLDPVTGENLGKPLKIPP----------DDLINIAGISQVPDGEASWHVTVSKYMDSPLL 314

Query: 195  SNGVVSFGFSIKAFV-------------DLFFTSIERQGGRLYLANTEGEILVTGFQNIR 254
            S  +  F  S K+ V              L    +E  GG +YL + EG +L T      
Sbjct: 315  SAALPVFDASNKSIVAVVGVTTALYSVGQLMRDLVEVHGGHIYLTSQEGYLLATSTDGPL 374

Query: 255  MVLTNGSASFQFLNPNGDQIARVGN---ISCLPRKEDFDARDSFFNLLGTKYMIYCSPLE 314
            +  T+          + + + + G          K           L   +Y I    L 
Sbjct: 375  LKNTSNGPQLMKATDSEEWVIKSGAQWLEKTYGSKRPHVVHAENVKLGDQRYYIDSFYLN 434

Query: 315  ILGVQLVYSLVLPQKELASLVYKSSRVGLILLILIMTTTVISIFGFVFIVI--RAAKREM 374
            +  + +V  +++P+K +   V    R    L+ILI  +  I   G V I+I      +EM
Sbjct: 435  LKRLPIVGVVIIPRKFIMGKV--DERAFKTLIILISASVCIFFIGCVCILILTNGVSKEM 494

Query: 375  YLCAKLIQQMEATQQAERKSMNKSVAFTQASHDIRASLAGIIGLIEICYNEAVPGSELDI 434
             L A+LI+Q++A ++AE  S  KS      SH++R  +A +IGL++I  ++    +E   
Sbjct: 495  KLRAELIRQLDARRRAEASSNYKSQFLANMSHELRTPMAAVIGLLDILISDDCLSNEQYA 554

Query: 435  NLKQMDGCTKDLLGILNSILDTSKIEAGKIQLEEEEFHLGQLLEDVVDLYHPVGMKKGID 494
             + Q+  C+  LL +LN+ILD SK+E+GK+ LEE EF LG+ LE +VD++    +   ++
Sbjct: 555  TVTQIRKCSTALLRLLNNILDLSKVESGKLVLEEAEFDLGRELEGLVDMFSVQCINHNVE 614

Query: 495  LVLDPYDGSVIKFSQVKGDRGKLKQVLCNLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMI 554
             VLD  D      + V+GD  +L Q+  NL+SN++KFT+ G + +R W +N+  +   M 
Sbjct: 615  TVLDLSDDMP---ALVRGDSARLVQIFANLISNSIKFTTTGHIILRGWCENINSLHDEMS 674

Query: 555  SSKHNGEILKHLSFLLCKNTERFREQQATDNGVHLNSDCMEFTFEIDDTGKGIPKEKRKL 614
             S    +  K  + +  K  +     Q +      N++ M   FE+DDTG GI   K   
Sbjct: 675  VSV---DRRKPWAPMKTKQVQHRNHLQKSCK----NANKMVLWFEVDDTGCGIDPSKWDS 734

Query: 615  VFENYVQV-KETALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVS 674
            VFE++ Q    T    GGTGLGL IV++LV  MGG+I ++ K     GT  R  ++L+  
Sbjct: 735  VFESFEQADPSTTRTHGGTGLGLCIVRNLVNKMGGEIKVVQK--NGLGTLMRLYLILS-- 794

Query: 675  VGNINSGEVTCQSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCK 734
                           T     Q   P      ++           V+L +     RM+  
Sbjct: 795  ---------------TPDTVDQNIQPDFSKYGLV-----------VMLSMYGSTARMITS 854

Query: 735  KFMETLGVKVLAMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGN 794
            K++   G+  +    W +L   ++ +LE                      S+  S DS +
Sbjct: 855  KWLRKHGIATVEASDWNELTQIIRDLLETG--------------------SRDNSFDSQH 914

Query: 795  GPNIDVSLGAMKDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQ 854
              NI   L A       L ++ +  +P   I   + V+D +   ++E  N +  F     
Sbjct: 915  --NISDPLRA------ELSNIVEIKNPVFVIVVDIGVLDLTTNIWKEQLNYLDRFSN--- 974

Query: 855  DAYCKVVWLLENQMSRII----NHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPE--- 914
                K  WLL++  S  +      KG        + ++++KP + +++ ++++ + +   
Sbjct: 975  --KAKFAWLLKHDTSNTVKTELRRKG--------HVMMVNKPLYKAKMIQILEAVIKNRK 1034

Query: 915  --FGGTLQSRESSRLYQIGSVSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSS 974
                  L++R +        +  DP+    Q+ + + + +S    G+   +V +  K S+
Sbjct: 1035 RGLCNDLRNRGNGSDESHDCLEIDPT----QFDTCSSDDSSET-SGE--KQVDKSVKPST 1094

Query: 975  GTSP--KNLSLNQIHSCLGSKSRISPVVGQQSRHQELREEKYENL----NGEKPLSGKKI 1034
              SP  KN  ++   S     S  + +  +    ++ ++  Y  +      +K L G +I
Sbjct: 1095 LHSPVLKNYLIDATTS--NDDSTSASMTQKNPEEEDWKDRLYSGIALDGKNQKSLEGIRI 1154

Query: 1035 LVAEDNLVLQKLARLNLERLGATIEICENGEEALELV--------------------CSG 1094
            L+AED  VLQ++A + LE++GAT+    +G++A++ +                     + 
Sbjct: 1155 LLAEDTPVLQRVATIMLEKMGATVTAVWDGQQAVDSLNYKSINAQAPTEEHKSFEEETAN 1192

Query: 1095 LGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQIRKMERYHNTHIPIIALTAHTTREE 1118
                R+    N+ PYD ILMDC+MP MDGYEAT+ IR+ E     HIPI+ALTAH    +
Sbjct: 1215 KVTTRETSLRNSSPYDLILMDCQMPKMDGYEATKAIRRAEIGTELHIPIVALTAHAMSSD 1192

BLAST of Spg003388 vs. TAIR 10
Match: AT2G01830.2 (CHASE domain containing histidine kinase protein )

HSP 1 Score: 138.7 bits (348), Expect = 3.0e-32
Identity = 197/837 (23.54%), Postives = 333/837 (39.78%), Query Frame = 0

Query: 319  ILLILIMTTTVISIFGFV--FIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQ 378
            I L ++ T  +    GF+  +I+  AA   + +     +  E   +AE   + KS     
Sbjct: 420  IPLNVLTTVPLFFAIGFLVGYILYGAAMHIVKVEDDFHEMQELKVRAEAADVAKSQFLAT 479

Query: 379  ASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGK 438
             SH+IR  + GI+G++ +  +  +  ++ D   +    C K L+ ++N +LD +KIEAGK
Sbjct: 480  VSHEIRTPMNGILGMLAMLLDTELSSTQRDY-AQTAQVCGKALIALINEVLDRAKIEAGK 539

Query: 439  IQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQ-VKGDRGKLKQVLC 498
            ++LE   F +  +L+DV+ L+      K I+L +   D    K  + VKGD G+ +Q++ 
Sbjct: 540  LELESVPFDIRSILDDVLSLFSEESRNKSIELAVFVSD----KVPEIVKGDSGRFRQIII 599

Query: 499  NLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNG------EILKHLSFLLCKNTER 558
            NL+ N+VKFT +G + V+  +      +S   ++ + G       + K  S+      E 
Sbjct: 600  NLVGNSVKFTEKGHIFVKVHLAEQSKDESEPKNALNGGVSEEMIVVSKQSSYNTLSGYEA 659

Query: 559  FREQQATDNGVHLNSD---CMEFTF--------EIDDTGKGIPKEKRKLVFENYVQV-KE 618
               + + D+  HL S+     EF           I+DTG GIP   +  VF  ++Q    
Sbjct: 660  ADGRNSWDSFKHLVSEEQSLSEFDISSNVRLMVSIEDTGIGIPLVAQGRVFMPFMQADSS 719

Query: 619  TALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTC 678
            T+   GGTG+GL I + LV LM G I  + +     G+ F F+ +L              
Sbjct: 720  TSRNYGGTGIGLSISKCLVELMRGQINFISRP--HIGSTFWFTAVLE------------- 779

Query: 679  QSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVL 738
            +    S +         H P       S  +  + I++     R  V +  M+ LG+ V 
Sbjct: 780  KCDKCSAINHMKKPNVEHLP-------STFKGMKAIVVDAKPVRAAVTRYHMKRLGINVD 839

Query: 739  AMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAM 798
             +   +   V              ++  R+G+  P+              P +D+     
Sbjct: 840  VVTSLKTAVVAA------------AAFERNGSPLPTK-------------PQLDM----- 899

Query: 799  KDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLE 858
                                  IL+  D+                           W   
Sbjct: 900  ----------------------ILVEKDS---------------------------W--- 959

Query: 859  NQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGS 918
                  I+ +  DS +  LN                            SR +  ++    
Sbjct: 960  ------ISTEDNDSEIRLLN----------------------------SRTNGNVHH--- 1019

Query: 919  VSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSP----KNLSLNQIHSCL 978
              K P  +L+  +                T  + D+  S+G +     K L  + I +CL
Sbjct: 1020 --KSPKLALFATN---------------ITNSEFDRAKSAGFADTVIMKPLRASMIGACL 1068

Query: 979  GSKSRISPVVGQQSRHQELREEKYENLNGEKP------LSGKKILVAEDNLVLQKLARLN 1038
                          +  ELR+ + ++  G  P      L+GKKILV +DN+V +++A   
Sbjct: 1080 -------------QQVLELRKTRQQHPEGSSPATLKSLLTGKKILVVDDNIVNRRVAAGA 1068

Query: 1039 LERLGATIEICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQI 1098
            L++ GA +   E+G+ AL L+      Q  H       +D   MD +MP MDG+EATRQI
Sbjct: 1140 LKKFGAEVVCAESGQVALGLL------QIPH------TFDACFMDIQMPQMDGFEATRQI 1068

Query: 1099 RKMERYHNT------HIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIA 1119
            R ME+          H+PI+A+TA          +++GMD ++ KP  +ENL + +A
Sbjct: 1200 RMMEKETKEKTNLEWHLPILAMTADVIHATYEECLKSGMDGYVSKPFEEENLYKSVA 1068

BLAST of Spg003388 vs. TAIR 10
Match: AT2G01830.3 (CHASE domain containing histidine kinase protein )

HSP 1 Score: 138.7 bits (348), Expect = 3.0e-32
Identity = 197/837 (23.54%), Postives = 333/837 (39.78%), Query Frame = 0

Query: 319  ILLILIMTTTVISIFGFV--FIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQ 378
            I L ++ T  +    GF+  +I+  AA   + +     +  E   +AE   + KS     
Sbjct: 397  IPLNVLTTVPLFFAIGFLVGYILYGAAMHIVKVEDDFHEMQELKVRAEAADVAKSQFLAT 456

Query: 379  ASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGK 438
             SH+IR  + GI+G++ +  +  +  ++ D   +    C K L+ ++N +LD +KIEAGK
Sbjct: 457  VSHEIRTPMNGILGMLAMLLDTELSSTQRDY-AQTAQVCGKALIALINEVLDRAKIEAGK 516

Query: 439  IQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQ-VKGDRGKLKQVLC 498
            ++LE   F +  +L+DV+ L+      K I+L +   D    K  + VKGD G+ +Q++ 
Sbjct: 517  LELESVPFDIRSILDDVLSLFSEESRNKSIELAVFVSD----KVPEIVKGDSGRFRQIII 576

Query: 499  NLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNG------EILKHLSFLLCKNTER 558
            NL+ N+VKFT +G + V+  +      +S   ++ + G       + K  S+      E 
Sbjct: 577  NLVGNSVKFTEKGHIFVKVHLAEQSKDESEPKNALNGGVSEEMIVVSKQSSYNTLSGYEA 636

Query: 559  FREQQATDNGVHLNSD---CMEFTF--------EIDDTGKGIPKEKRKLVFENYVQV-KE 618
               + + D+  HL S+     EF           I+DTG GIP   +  VF  ++Q    
Sbjct: 637  ADGRNSWDSFKHLVSEEQSLSEFDISSNVRLMVSIEDTGIGIPLVAQGRVFMPFMQADSS 696

Query: 619  TALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTC 678
            T+   GGTG+GL I + LV LM G I  + +     G+ F F+ +L              
Sbjct: 697  TSRNYGGTGIGLSISKCLVELMRGQINFISRP--HIGSTFWFTAVLE------------- 756

Query: 679  QSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVL 738
            +    S +         H P       S  +  + I++     R  V +  M+ LG+ V 
Sbjct: 757  KCDKCSAINHMKKPNVEHLP-------STFKGMKAIVVDAKPVRAAVTRYHMKRLGINVD 816

Query: 739  AMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAM 798
             +   +   V              ++  R+G+  P+              P +D+     
Sbjct: 817  VVTSLKTAVVAA------------AAFERNGSPLPTK-------------PQLDM----- 876

Query: 799  KDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLE 858
                                  IL+  D+                           W   
Sbjct: 877  ----------------------ILVEKDS---------------------------W--- 936

Query: 859  NQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGS 918
                  I+ +  DS +  LN                            SR +  ++    
Sbjct: 937  ------ISTEDNDSEIRLLN----------------------------SRTNGNVHH--- 996

Query: 919  VSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSP----KNLSLNQIHSCL 978
              K P  +L+  +                T  + D+  S+G +     K L  + I +CL
Sbjct: 997  --KSPKLALFATN---------------ITNSEFDRAKSAGFADTVIMKPLRASMIGACL 1045

Query: 979  GSKSRISPVVGQQSRHQELREEKYENLNGEKP------LSGKKILVAEDNLVLQKLARLN 1038
                          +  ELR+ + ++  G  P      L+GKKILV +DN+V +++A   
Sbjct: 1057 -------------QQVLELRKTRQQHPEGSSPATLKSLLTGKKILVVDDNIVNRRVAAGA 1045

Query: 1039 LERLGATIEICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQI 1098
            L++ GA +   E+G+ AL L+      Q  H       +D   MD +MP MDG+EATRQI
Sbjct: 1117 LKKFGAEVVCAESGQVALGLL------QIPH------TFDACFMDIQMPQMDGFEATRQI 1045

Query: 1099 RKMERYHNT------HIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIA 1119
            R ME+          H+PI+A+TA          +++GMD ++ KP  +ENL + +A
Sbjct: 1177 RMMEKETKEKTNLEWHLPILAMTADVIHATYEECLKSGMDGYVSKPFEEENLYKSVA 1045

BLAST of Spg003388 vs. TAIR 10
Match: AT2G01830.1 (CHASE domain containing histidine kinase protein )

HSP 1 Score: 138.7 bits (348), Expect = 3.0e-32
Identity = 197/837 (23.54%), Postives = 333/837 (39.78%), Query Frame = 0

Query: 319  ILLILIMTTTVISIFGFV--FIVIRAAKREMYLCAKLIQQMEATQQAERKSMNKSVAFTQ 378
            I L ++ T  +    GF+  +I+  AA   + +     +  E   +AE   + KS     
Sbjct: 397  IPLNVLTTVPLFFAIGFLVGYILYGAAMHIVKVEDDFHEMQELKVRAEAADVAKSQFLAT 456

Query: 379  ASHDIRASLAGIIGLIEICYNEAVPGSELDINLKQMDGCTKDLLGILNSILDTSKIEAGK 438
             SH+IR  + GI+G++ +  +  +  ++ D   +    C K L+ ++N +LD +KIEAGK
Sbjct: 457  VSHEIRTPMNGILGMLAMLLDTELSSTQRDY-AQTAQVCGKALIALINEVLDRAKIEAGK 516

Query: 439  IQLEEEEFHLGQLLEDVVDLYHPVGMKKGIDLVLDPYDGSVIKFSQ-VKGDRGKLKQVLC 498
            ++LE   F +  +L+DV+ L+      K I+L +   D    K  + VKGD G+ +Q++ 
Sbjct: 517  LELESVPFDIRSILDDVLSLFSEESRNKSIELAVFVSD----KVPEIVKGDSGRFRQIII 576

Query: 499  NLLSNAVKFTSEGQVTVRAWVKNLPDMQSNMISSKHNG------EILKHLSFLLCKNTER 558
            NL+ N+VKFT +G + V+  +      +S   ++ + G       + K  S+      E 
Sbjct: 577  NLVGNSVKFTEKGHIFVKVHLAEQSKDESEPKNALNGGVSEEMIVVSKQSSYNTLSGYEA 636

Query: 559  FREQQATDNGVHLNSD---CMEFTF--------EIDDTGKGIPKEKRKLVFENYVQV-KE 618
               + + D+  HL S+     EF           I+DTG GIP   +  VF  ++Q    
Sbjct: 637  ADGRNSWDSFKHLVSEEQSLSEFDISSNVRLMVSIEDTGIGIPLVAQGRVFMPFMQADSS 696

Query: 619  TALGQGGTGLGLGIVQSLVRLMGGDIAILDKEIGEKGTCFRFSVLLTVSVGNINSGEVTC 678
            T+   GGTG+GL I + LV LM G I  + +     G+ F F+ +L              
Sbjct: 697  TSRNYGGTGIGLSISKCLVELMRGQINFISRP--HIGSTFWFTAVLE------------- 756

Query: 679  QSSPTSRLTFQAPSPSLHSPRMIRTTGSKTETSRVILLIRNDQRRMVCKKFMETLGVKVL 738
            +    S +         H P       S  +  + I++     R  V +  M+ LG+ V 
Sbjct: 757  KCDKCSAINHMKKPNVEHLP-------STFKGMKAIVVDAKPVRAAVTRYHMKRLGINVD 816

Query: 739  AMKQWEQLQVTLQKILEKQSHSRHSSRGRSGNSSPSDCLSKSTSGDSGNGPNIDVSLGAM 798
             +   +   V              ++  R+G+  P+              P +D+     
Sbjct: 817  VVTSLKTAVVAA------------AAFERNGSPLPTK-------------PQLDM----- 876

Query: 799  KDETNYLLSVFKKTSPRGGISFILIVIDASAGPFREICNMVASFRTGLQDAYCKVVWLLE 858
                                  IL+  D+                           W   
Sbjct: 877  ----------------------ILVEKDS---------------------------W--- 936

Query: 859  NQMSRIINHKGLDSSVFELNDVVISKPFHGSRLYEVIKLLPEFGGTLQSRESSRLYQIGS 918
                  I+ +  DS +  LN                            SR +  ++    
Sbjct: 937  ------ISTEDNDSEIRLLN----------------------------SRTNGNVHH--- 996

Query: 919  VSKDPSSSLYQYHSKAKEGNSPIFRGQIATRVQQDKKSSSGTSP----KNLSLNQIHSCL 978
              K P  +L+  +                T  + D+  S+G +     K L  + I +CL
Sbjct: 997  --KSPKLALFATN---------------ITNSEFDRAKSAGFADTVIMKPLRASMIGACL 1045

Query: 979  GSKSRISPVVGQQSRHQELREEKYENLNGEKP------LSGKKILVAEDNLVLQKLARLN 1038
                          +  ELR+ + ++  G  P      L+GKKILV +DN+V +++A   
Sbjct: 1057 -------------QQVLELRKTRQQHPEGSSPATLKSLLTGKKILVVDDNIVNRRVAAGA 1045

Query: 1039 LERLGATIEICENGEEALELVCSGLGNQRKHGASNTLPYDFILMDCEMPVMDGYEATRQI 1098
            L++ GA +   E+G+ AL L+      Q  H       +D   MD +MP MDG+EATRQI
Sbjct: 1117 LKKFGAEVVCAESGQVALGLL------QIPH------TFDACFMDIQMPQMDGFEATRQI 1045

Query: 1099 RKMERYHNT------HIPIIALTAHTTREEARRTIEAGMDVHLGKPLRKENLLEVIA 1119
            R ME+          H+PI+A+TA          +++GMD ++ KP  +ENL + +A
Sbjct: 1177 RMMEKETKEKTNLEWHLPILAMTADVIHATYEECLKSGMDGYVSKPFEEENLYKSVA 1045

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0048070.10.0e+0081.83histidine kinase CKI1-like [Cucumis melo var. makuwa][more]
XP_004144811.20.0e+0081.97histidine kinase CKI1 [Cucumis sativus][more]
XP_022965407.10.0e+0080.21histidine kinase CKI1 [Cucurbita maxima][more]
XP_038889343.10.0e+0080.04histidine kinase CKI1 [Benincasa hispida][more]
XP_023537978.10.0e+0079.34histidine kinase CKI1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O222676.1e-20039.34Histidine kinase CKI1 OS=Arabidopsis thaliana OX=3702 GN=CKI1 PE=1 SV=1[more]
A2YA151.6e-12030.84Probable histidine kinase 2 OS=Oryza sativa subsp. indica OX=39946 GN=HK2 PE=3 S... [more]
Q5SML41.6e-12030.84Probable histidine kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=HK2 PE=2... [more]
Q9SXL43.9e-6925.13Histidine kinase 1 OS=Arabidopsis thaliana OX=3702 GN=AHK1 PE=1 SV=2[more]
A1A6983.1e-3424.10Probable histidine kinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=HK4 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7TXY40.0e+0081.83Histidine kinase CKI1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A6J1HK930.0e+0080.21histidine kinase CKI1 OS=Cucurbita maxima OX=3661 GN=LOC111465321 PE=4 SV=1[more]
A0A0A0LM390.0e+0082.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034540 PE=4 SV=1[more]
A0A6J1FCT00.0e+0079.27histidine kinase CKI1 OS=Cucurbita moschata OX=3662 GN=LOC111444446 PE=4 SV=1[more]
A0A1S3BWD00.0e+0081.73LOW QUALITY PROTEIN: histidine kinase CKI1-like OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT2G47430.14.3e-20139.34Signal transduction histidine kinase [more]
AT2G17820.12.7e-7025.13histidine kinase 1 [more]
AT2G01830.23.0e-3223.54CHASE domain containing histidine kinase protein [more]
AT2G01830.33.0e-3223.54CHASE domain containing histidine kinase protein [more]
AT2G01830.13.0e-3223.54CHASE domain containing histidine kinase protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004358Signal transduction histidine kinase-related protein, C-terminalPRINTSPR00344BCTRLSENSORcoord: 569..583
score: 37.51
coord: 587..597
score: 23.81
coord: 603..621
score: 50.02
IPR001789Signal transduction response regulator, receiver domainSMARTSM00448REC_2coord: 989..1117
e-value: 1.2E-31
score: 121.1
IPR001789Signal transduction response regulator, receiver domainPFAMPF00072Response_regcoord: 991..1117
e-value: 4.3E-21
score: 75.2
IPR001789Signal transduction response regulator, receiver domainPROSITEPS50110RESPONSE_REGULATORYcoord: 990..1121
score: 38.445618
IPR003661Signal transduction histidine kinase, dimerisation/phosphoacceptor domainSMARTSM00388HisKA_10coord: 369..435
e-value: 4.0E-6
score: 36.3
IPR003661Signal transduction histidine kinase, dimerisation/phosphoacceptor domainCDDcd00082HisKAcoord: 377..431
e-value: 3.03706E-5
score: 41.044
IPR003594Histidine kinase/HSP90-like ATPaseSMARTSM00387HKATPase_4coord: 485..645
e-value: 5.1E-31
score: 119.0
IPR003594Histidine kinase/HSP90-like ATPasePFAMPF02518HATPase_ccoord: 485..643
e-value: 8.2E-25
score: 87.5
NoneNo IPR availableGENE3D1.10.287.130coord: 356..435
e-value: 3.7E-11
score: 44.9
NoneNo IPR availableGENE3D3.40.50.2300coord: 966..1122
e-value: 1.0E-43
score: 150.6
NoneNo IPR availablePIRSRPIRSR002937-1PIRSR002937-1coord: 1045..1120
e-value: 9.5E-5
score: 19.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 736..772
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 661..680
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 743..768
NoneNo IPR availablePANTHERPTHR43719TWO-COMPONENT HISTIDINE KINASEcoord: 6..1118
NoneNo IPR availablePANTHERPTHR43719:SF50HISTIDINE KINASE CKI1-LIKEcoord: 6..1118
NoneNo IPR availableCDDcd17546REC_hyHK_CKI1_RcsC-likecoord: 991..1117
e-value: 6.05546E-46
score: 158.402
IPR036890Histidine kinase/HSP90-like ATPase superfamilyGENE3D3.30.565.10coord: 436..647
e-value: 8.8E-43
score: 147.7
IPR036890Histidine kinase/HSP90-like ATPase superfamilySUPERFAMILY55874ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinasecoord: 424..641
IPR005467Histidine kinase domainPROSITEPS50109HIS_KINcoord: 376..645
score: 37.268234
IPR036097Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamilySUPERFAMILY47384Homodimeric domain of signal transducing histidine kinasecoord: 357..436
IPR011006CheY-like superfamilySUPERFAMILY52172CheY-likecoord: 985..1118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003388.1Spg003388.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048856 anatomical structure development
biological_process GO:0000160 phosphorelay signal transduction system
biological_process GO:0006468 protein phosphorylation
biological_process GO:0042221 response to chemical
biological_process GO:0016310 phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000155 phosphorelay sensor kinase activity
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups