Spg003383 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003383 vs. NCBI nr
Match: XP_004139365.1 (GDSL esterase/lipase At1g54790 [Cucumis sativus] >KGN60875.1 hypothetical protein Csa_023481 [Cucumis sativus])

HSP 1 Score: 612.8 bits (1579), Expect = 1.8e-171
Identity = 302/376 (80.32%), Postives = 322/376 (85.64%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTIF+ GSS+  DYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYF S SSGRF
Sbjct: 16  MLTMCSTIFRSGSSAGFDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFQSPSSGRF 75

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSF+KGCN+AAGGST+LPA AAFISPFSFGVQ
Sbjct: 76  CNGRLIIDFLMEATGMPYLRAYLDSVGRPSFKKGCNYAAGGSTVLPATAAFISPFSFGVQ 135

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QFLHFK+RVLQLRA+GDKKI KFLPVE YFK G+YMFDIGQNDL + FYS  S DQAI
Sbjct: 136 INQFLHFKSRVLQLRAQGDKKIGKFLPVEKYFKDGIYMFDIGQNDLTAAFYSKASMDQAI 195

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEF+ GLQ+LYD+GARNFWIHN+GPLGCLAQNIA FG  DPSKLD+ GCL SHNQA
Sbjct: 196 PTILTEFEIGLQKLYDQGARNFWIHNTGPLGCLAQNIATFGT-DPSKLDEFGCLTSHNQA 255

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           AKLFNSQLH LSKKLQ+QY DAT+ YVDIY IK NLIANYSQLGFEQPIM CCG GGPPL
Sbjct: 256 AKLFNSQLHDLSKKLQSQYVDATIIYVDIYTIKFNLIANYSQLGFEQPIMTCCGFGGPPL 315

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 359
           NYDSRISCGLTK LNGTVVTA GC DSSKYVNWDG HYTEAANEYVSSQILTGKY DPPF
Sbjct: 316 NYDSRISCGLTKTLNGTVVTANGCKDSSKYVNWDGVHYTEAANEYVSSQILTGKYCDPPF 375

BLAST of Spg003383 vs. NCBI nr
Match: KAA0063685.1 (GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK18342.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 610.9 bits (1574), Expect = 7.0e-171
Identity = 302/376 (80.32%), Postives = 322/376 (85.64%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           +LTMCSTIF+ GSS+  DYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYF S SSGRF
Sbjct: 16  ILTMCSTIFRSGSSAGFDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFQSPSSGRF 75

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSFRKGCN+AAGGSTILP  AA ISPFSFGVQ
Sbjct: 76  CNGRLIIDFLMEATGLPYLRAYLDSVGRPSFRKGCNYAAGGSTILPGTAASISPFSFGVQ 135

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QF+HFK+RVLQLRA+GDKKI KFLPVE YFK GVYMFDIGQNDL + FYS  S DQAI
Sbjct: 136 INQFIHFKSRVLQLRAQGDKKIGKFLPVEKYFKDGVYMFDIGQNDLTAAFYSKASMDQAI 195

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEF+ GLQ+LY++GARNFWIHN+GPLGCLAQNIA FG  DPSKLD+ GCL SHNQA
Sbjct: 196 PTILTEFEIGLQKLYEQGARNFWIHNTGPLGCLAQNIATFG-SDPSKLDEFGCLTSHNQA 255

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           AKLFNSQLH LSKKLQ+QY DATVTYVDIY IK NLIANYSQLGFEQPIM CCG GGPPL
Sbjct: 256 AKLFNSQLHDLSKKLQSQYVDATVTYVDIYTIKFNLIANYSQLGFEQPIMTCCGFGGPPL 315

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 359
           NYDSR+SCGLTK LNGTVVTA GCDDSSKYVNWDG HYTEAANEYVSSQILTGKY DPPF
Sbjct: 316 NYDSRLSCGLTKTLNGTVVTANGCDDSSKYVNWDGVHYTEAANEYVSSQILTGKYCDPPF 375

BLAST of Spg003383 vs. NCBI nr
Match: XP_008455044.1 (PREDICTED: GDSL esterase/lipase At1g54790-like [Cucumis melo])

HSP 1 Score: 609.8 bits (1571), Expect = 1.6e-170
Identity = 302/376 (80.32%), Postives = 321/376 (85.37%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTIF+ GSS+  DYPAVFNFGDSNSDTGELLAGKGFSL LPYGETYF S SSGRF
Sbjct: 16  MLTMCSTIFRSGSSAGFDYPAVFNFGDSNSDTGELLAGKGFSLLLPYGETYFQSPSSGRF 75

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSFRKGCN+AAGGSTILP  AA ISPFSFGVQ
Sbjct: 76  CNGRLIIDFLMEATGLPYLRAYLDSVGRPSFRKGCNYAAGGSTILPGTAASISPFSFGVQ 135

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QF+HFK+RVLQLRA+GDKKI KFLPVE YFK GVYMFDIGQNDL + FYS  S DQAI
Sbjct: 136 INQFIHFKSRVLQLRAQGDKKIGKFLPVEKYFKDGVYMFDIGQNDLTAAFYSKASMDQAI 195

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEF+ GLQ+LY++GARNFWIHN+GPLGCLAQNIA FG  DPSKLD+ GCL SHNQA
Sbjct: 196 PTILTEFEIGLQKLYEQGARNFWIHNTGPLGCLAQNIATFG-SDPSKLDEFGCLTSHNQA 255

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           AKLFNSQLH LSKKLQ+QY DATVTYVDIY IK NLIANYSQLGFEQPIM CCG GGPPL
Sbjct: 256 AKLFNSQLHDLSKKLQSQYVDATVTYVDIYTIKFNLIANYSQLGFEQPIMTCCGFGGPPL 315

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 359
           NYDSR+SCGLTK LNGTVVTA GCDDSSKYVNWDG HYTEAANEYVSSQILTGKY DPPF
Sbjct: 316 NYDSRLSCGLTKTLNGTVVTANGCDDSSKYVNWDGVHYTEAANEYVSSQILTGKYCDPPF 375

BLAST of Spg003383 vs. NCBI nr
Match: XP_022999118.1 (GDSL esterase/lipase At1g54790-like [Cucurbita maxima])

HSP 1 Score: 599.0 bits (1543), Expect = 2.7e-167
Identity = 295/372 (79.30%), Postives = 319/372 (85.75%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTIF+ GSSSV  YPAVFNFGDSNSDTG+LLAGKGFSL LPYGETYF S SSGRF
Sbjct: 14  MLTMCSTIFRSGSSSVFHYPAVFNFGDSNSDTGDLLAGKGFSLPLPYGETYFQSPSSGRF 73

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSFRKGCN+AAGGST+LPA AA +SPF+FGVQ
Sbjct: 74  CNGRLIIDFLMEAMGKPHLRAYLESVGRPSFRKGCNYAAGGSTVLPATAASVSPFAFGVQ 133

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QFLHFK RVL LRAKG+KK++K LPVE  FKKGVYMFDIGQNDL + FYS T  DQAI
Sbjct: 134 LNQFLHFKDRVLLLRAKGNKKVNKLLPVEKSFKKGVYMFDIGQNDLTAAFYSKTPLDQAI 193

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEFQ GLQRLY+ GAR+FWIHN+GPLGCLAQNIA FG  DPSKLD+ GCLISHNQA
Sbjct: 194 PTILTEFQTGLQRLYENGARHFWIHNTGPLGCLAQNIATFG-SDPSKLDEFGCLISHNQA 253

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           A LFNSQLHAL K LQ+QY DATVTYVDIYAIK NL+ANYSQLGFEQPIM CCG GGPPL
Sbjct: 254 ANLFNSQLHALCKTLQSQYVDATVTYVDIYAIKFNLVANYSQLGFEQPIMTCCGYGGPPL 313

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 357
           NYD+RISCGLTK L+G VVTAKGC+DSSKYVNWDGTHYTEAANEYVSSQILTGKY+DPPF
Sbjct: 314 NYDNRISCGLTKTLDGKVVTAKGCNDSSKYVNWDGTHYTEAANEYVSSQILTGKYSDPPF 373

BLAST of Spg003383 vs. NCBI nr
Match: XP_023545470.1 (GDSL esterase/lipase At1g54790-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545471.1 GDSL esterase/lipase At1g54790-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.5 bits (1534), Expect = 3.0e-166
Identity = 292/372 (78.49%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTI +CGSSSV  YPAVFNFGDSNSDTGELLAGKGF L LPYGETYF S SSGRF
Sbjct: 14  MLTMCSTICRCGSSSVFHYPAVFNFGDSNSDTGELLAGKGFRLPLPYGETYFQSPSSGRF 73

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLI+DFLM               G+PSFRKGCN+AAGGST+LPA AA ISPF+FGVQ
Sbjct: 74  CNGRLIVDFLMEAMGKPHLRAYLESVGRPSFRKGCNYAAGGSTVLPATAASISPFAFGVQ 133

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QFLHFK RVL+LRAKG+KK++K LPVE  FKKGVYMFDIGQNDL + FYS T  DQAI
Sbjct: 134 LNQFLHFKDRVLRLRAKGNKKVNKLLPVEKSFKKGVYMFDIGQNDLTAAFYSKTPLDQAI 193

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEFQ GLQRLY+ GAR+FWIHN+GPLGCLAQNIA FG  DPSKLD+ GCLISHNQA
Sbjct: 194 PTILTEFQTGLQRLYENGARHFWIHNTGPLGCLAQNIATFGT-DPSKLDEFGCLISHNQA 253

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           A LFNSQLHAL K LQ+QY DATVTY+DIYAIK NL+ANYSQLGFEQPIM CCG GGPPL
Sbjct: 254 ANLFNSQLHALCKTLQSQYVDATVTYIDIYAIKFNLVANYSQLGFEQPIMTCCGYGGPPL 313

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 357
           NYD+RI+CGLTK L+G VVTAKGC+DSSKYVNWDGTHYTEAANEYVSSQILT KY+DPPF
Sbjct: 314 NYDNRITCGLTKTLDGKVVTAKGCNDSSKYVNWDGTHYTEAANEYVSSQILTEKYSDPPF 373

BLAST of Spg003383 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 5.4e-126
Identity = 229/392 (58.42%), Postives = 281/392 (71.68%), Query Frame = 0

Query: 10  QCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDF 69
           Q  +S   +YP+ FNFGDSNSDTG+L+AG G  L LP G+  F + SS RFC+GRL+IDF
Sbjct: 20  QISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKT-SSQRFCDGRLVIDF 79

Query: 70  LM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKA 129
           LM               G P+F+KGCNFAA GSTILPAN   +SPFSF +Q+SQF+ FK+
Sbjct: 80  LMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFKS 139

Query: 130 RVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPD--QAIPTILTEF 189
           R ++L +K  +K +K+LP  +Y+ KG+YM DIGQND+   FYS T      +IP+IL  F
Sbjct: 140 RAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETF 199

Query: 190 QAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQAAKLFNSQ 249
           +AGL+RLY+EG RN WIHN+GPLGCLAQNIA FG  D +KLD+ GC+ SHNQAAKLFN Q
Sbjct: 200 EAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGT-DSTKLDEFGCVSSHNQAAKLFNLQ 259

Query: 250 LHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQ------------------------- 309
           LHA+S K QAQY DA VTYVDI++IK NLIANYS+                         
Sbjct: 260 LHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNHLENVGYNKILN 319

Query: 310 -LGFEQPIMACCGNGGPPLNYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEA 359
            LGFE+P+MACCG GG PLNYDSRI+CG TK+L+G  VTAK C+DSS+Y+NWDG HYTEA
Sbjct: 320 VLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEA 379

BLAST of Spg003383 vs. ExPASy Swiss-Prot
Match: Q9MAA1 (GDSL esterase/lipase At3g05180 OS=Arabidopsis thaliana OX=3702 GN=At3g05180 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.6e-114
Identity = 204/349 (58.45%), Postives = 254/349 (72.78%), Query Frame = 0

Query: 18  DYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDFLM------ 77
           D+PAVFNFGDSNSDTGEL +G GF  +  Y  T+F S +SGRFCNGRLI+DFLM      
Sbjct: 33  DFPAVFNFGDSNSDTGELSSGLGFLPQPSYEITFFRSPTSGRFCNGRLIVDFLMEAIDRP 92

Query: 78  ---------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKARVLQLRAK 137
                     + ++R+GCNFAA  STI  ANAA  SPF FGVQVSQF+ FK++VLQL  +
Sbjct: 93  YLRPYLDSISRQTYRRGCNFAAAASTIQKANAASYSPFGFGVQVSQFITFKSKVLQL-IQ 152

Query: 138 GDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQA---IPTILTEFQAGLQRL 197
            D+++ ++LP E +F  G+YMFDIGQND+   FY T + DQ    +P IL  FQ G++RL
Sbjct: 153 QDEELQRYLPSEYFFSNGLYMFDIGQNDIAGAFY-TKTVDQVLALVPIILDIFQDGIKRL 212

Query: 198 YDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQAAKLFNSQLHALSKK 257
           Y EGARN+WIHN+GPLGCLAQ +++FG+ D SKLD+ GC+  HNQAAKLFN QLH L KK
Sbjct: 213 YAEGARNYWIHNTGPLGCLAQVVSIFGE-DKSKLDEFGCVSDHNQAAKLFNLQLHGLFKK 272

Query: 258 LQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPLNYDSRISCGLTKML 317
           L  QY ++  TYVDI++IK +LI N+S+ GF+  IM CCG GGPPLNYD ++ CG T   
Sbjct: 273 LPQQYPNSRFTYVDIFSIKSDLILNHSKYGFDHSIMVCCGTGGPPLNYDDQVGCGKTARS 332

Query: 318 NGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPFSSN 349
           NGT++TAK C DSSKYVNWDG HYTEAAN +V+  ILTGKY++   S N
Sbjct: 333 NGTIITAKPCYDSSKYVNWDGIHYTEAANRFVALHILTGKYSETASSLN 378

BLAST of Spg003383 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.4e-80
Identity = 156/343 (45.48%), Postives = 212/343 (61.81%), Query Frame = 0

Query: 20  PAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDFL--------- 79
           P +FNFGDSNSDTG L+AG G+ +  P G  +F   S+GR  +GRL+IDFL         
Sbjct: 39  PVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFR-RSTGRLSDGRLLIDFLCQSLNTSLL 98

Query: 80  ------MGKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKARVLQLRAKGD 139
                 +G+  F+ G NFA  GS  LP N     PFS  +QV QF HFK+R L+L +  +
Sbjct: 99  RPYLDSLGRTRFQNGANFAIAGSPTLPKNV----PFSLNIQVKQFSHFKSRSLELASSSN 158

Query: 140 KKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQA---IPTILTEFQAGLQRLYD 199
                F+   N FK  +YM DIGQND+   F    S  Q    IP I+TE ++ ++RLYD
Sbjct: 159 SLKGMFIS-NNGFKNALYMIDIGQNDIARSFARGNSYSQTVKLIPQIITEIKSSIKRLYD 218

Query: 200 EGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQAAKLFNSQLHALSKKLQ 259
           EG R FWIHN+GPLGCL Q +++    D   LD  GCL+S+N AA LFN  L  + ++L+
Sbjct: 219 EGGRRFWIHNTGPLGCLPQKLSMVKSKD---LDQHGCLVSYNSAATLFNQGLDHMCEELR 278

Query: 260 AQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPLNYDSRISCGLTKMLNG 319
            +  DAT+ Y+DIYAIK +LIAN +Q GF+ P+MACCG GG P NY+ +I+CG      G
Sbjct: 279 TELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMACCGYGGTPYNYNVKITCG----HKG 338

Query: 320 TVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPP 345
           + V    C++ S++++WDG HYTE AN  V+ ++L+  Y+ PP
Sbjct: 339 SNV----CEEGSRFISWDGIHYTETANAIVAMKVLSMHYSKPP 364

BLAST of Spg003383 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.7e-77
Identity = 151/344 (43.90%), Postives = 215/344 (62.50%), Query Frame = 0

Query: 20  PAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDFL--------- 79
           P +FNFGDSNSDTG L+AG G+S+ LP G ++F   S+GR  +GRL+IDFL         
Sbjct: 36  PVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFF-QRSTGRLSDGRLVIDFLCQSLNTSLL 95

Query: 80  ------MGKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKARVLQLRAKGD 139
                 +    F+ G NFA  GS+ LP       PF+  +Q+ QFLHFK+R L+L +  D
Sbjct: 96  NPYLDSLVGSKFQNGANFAIVGSSTLPRYV----PFALNIQLMQFLHFKSRALELASISD 155

Query: 140 KKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQA---IPTILTEFQAGLQRLYD 199
             + + +  E+ F+  +YM DIGQND+   F    S  +    IP +++E ++ ++ LYD
Sbjct: 156 -PLKEMMIGESGFRNALYMIDIGQNDIADSFSKGLSYSRVVKLIPNVISEIKSAIKILYD 215

Query: 200 EGARNFWIHNSGPLGCLAQNIA-VFGKGDPSKLDDLGCLISHNQAAKLFNSQLHALSKKL 259
           EG R FW+HN+GPLGCL Q ++ V  KG     D  GCL ++N AAKLFN  L  + + L
Sbjct: 216 EGGRKFWVHNTGPLGCLPQKLSMVHSKG----FDKHGCLATYNAAAKLFNEGLDHMCRDL 275

Query: 260 QAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPLNYDSRISCGLTKMLN 319
           + +  +A + YVDIYAIK +LIAN +  GFE+P+MACCG GGPP NY+  I+CG     N
Sbjct: 276 RTELKEANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYGGPPYNYNVNITCG-----N 335

Query: 320 GTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPP 345
           G    +K CD+ S++++WDG HYTE AN  V+ ++L+ +++ PP
Sbjct: 336 G---GSKSCDEGSRFISWDGIHYTETANAIVAMKVLSMQHSTPP 361

BLAST of Spg003383 vs. ExPASy Swiss-Prot
Match: Q6NLP7 (GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.5e-71
Identity = 150/370 (40.54%), Postives = 213/370 (57.57%), Query Frame = 0

Query: 2   LTMCSTIFQCGSSSVL----DYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASS 61
           L +C ++  C +S         P + NFGDSNSDTG +LAG G  + LP+G T+F+   +
Sbjct: 13  LVLCLSLLVCSNSETSYKSNKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFH-RGT 72

Query: 62  GRFCNGRLIIDFLMG--------------KPSFRKGCNFAAGGSTILPANAAFISPFSFG 121
           GR  +GRLI+DF                  P+F++G NFA  G+T LP     I  F   
Sbjct: 73  GRLGDGRLIVDFYCEHLKMTYLSPYLDSLSPNFKRGVNFAVSGATALP-----IFSFPLA 132

Query: 122 VQVSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYS---TTS 181
           +Q+ QF+HFK R  +L + G +     L  +N F+  +YM DIGQNDL+   Y    T +
Sbjct: 133 IQIRQFVHFKNRSQELISSGRRD----LIDDNGFRNALYMIDIGQNDLLLALYDSNLTYA 192

Query: 182 P-DQAIPTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCL 241
           P  + IP++L E +  +Q +Y  G R FW+HN+GPLGC  + +A+    D S LD +GC 
Sbjct: 193 PVVEKIPSMLLEIKKAIQTVYLYGGRKFWVHNTGPLGCAPKELAIHLHND-SDLDPIGCF 252

Query: 242 ISHNQAAKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCG 301
             HN+ AK FN  L +L  +L++Q+ DAT+ YVDIY+IK  L A++   GF  P+MACCG
Sbjct: 253 RVHNEVAKAFNKGLLSLCNELRSQFKDATLVYVDIYSIKYKLSADFKLYGFVDPLMACCG 312

Query: 302 NGGPPLNYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGK 350
            GG P NYD + +CG      G+ +    C D +K + WDG HYTEAAN +V   +LT +
Sbjct: 313 YGGRPNNYDRKATCGQP----GSTI----CRDVTKAIVWDGVHYTEAANRFVVDAVLTNR 363

BLAST of Spg003383 vs. ExPASy TrEMBL
Match: A0A0A0LFP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G020970 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.9e-172
Identity = 302/376 (80.32%), Postives = 322/376 (85.64%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTIF+ GSS+  DYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYF S SSGRF
Sbjct: 16  MLTMCSTIFRSGSSAGFDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFQSPSSGRF 75

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSF+KGCN+AAGGST+LPA AAFISPFSFGVQ
Sbjct: 76  CNGRLIIDFLMEATGMPYLRAYLDSVGRPSFKKGCNYAAGGSTVLPATAAFISPFSFGVQ 135

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QFLHFK+RVLQLRA+GDKKI KFLPVE YFK G+YMFDIGQNDL + FYS  S DQAI
Sbjct: 136 INQFLHFKSRVLQLRAQGDKKIGKFLPVEKYFKDGIYMFDIGQNDLTAAFYSKASMDQAI 195

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEF+ GLQ+LYD+GARNFWIHN+GPLGCLAQNIA FG  DPSKLD+ GCL SHNQA
Sbjct: 196 PTILTEFEIGLQKLYDQGARNFWIHNTGPLGCLAQNIATFGT-DPSKLDEFGCLTSHNQA 255

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           AKLFNSQLH LSKKLQ+QY DAT+ YVDIY IK NLIANYSQLGFEQPIM CCG GGPPL
Sbjct: 256 AKLFNSQLHDLSKKLQSQYVDATIIYVDIYTIKFNLIANYSQLGFEQPIMTCCGFGGPPL 315

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 359
           NYDSRISCGLTK LNGTVVTA GC DSSKYVNWDG HYTEAANEYVSSQILTGKY DPPF
Sbjct: 316 NYDSRISCGLTKTLNGTVVTANGCKDSSKYVNWDGVHYTEAANEYVSSQILTGKYCDPPF 375

BLAST of Spg003383 vs. ExPASy TrEMBL
Match: A0A5D3D478 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00570 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.4e-171
Identity = 302/376 (80.32%), Postives = 322/376 (85.64%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           +LTMCSTIF+ GSS+  DYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYF S SSGRF
Sbjct: 16  ILTMCSTIFRSGSSAGFDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFQSPSSGRF 75

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSFRKGCN+AAGGSTILP  AA ISPFSFGVQ
Sbjct: 76  CNGRLIIDFLMEATGLPYLRAYLDSVGRPSFRKGCNYAAGGSTILPGTAASISPFSFGVQ 135

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QF+HFK+RVLQLRA+GDKKI KFLPVE YFK GVYMFDIGQNDL + FYS  S DQAI
Sbjct: 136 INQFIHFKSRVLQLRAQGDKKIGKFLPVEKYFKDGVYMFDIGQNDLTAAFYSKASMDQAI 195

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEF+ GLQ+LY++GARNFWIHN+GPLGCLAQNIA FG  DPSKLD+ GCL SHNQA
Sbjct: 196 PTILTEFEIGLQKLYEQGARNFWIHNTGPLGCLAQNIATFG-SDPSKLDEFGCLTSHNQA 255

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           AKLFNSQLH LSKKLQ+QY DATVTYVDIY IK NLIANYSQLGFEQPIM CCG GGPPL
Sbjct: 256 AKLFNSQLHDLSKKLQSQYVDATVTYVDIYTIKFNLIANYSQLGFEQPIMTCCGFGGPPL 315

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 359
           NYDSR+SCGLTK LNGTVVTA GCDDSSKYVNWDG HYTEAANEYVSSQILTGKY DPPF
Sbjct: 316 NYDSRLSCGLTKTLNGTVVTANGCDDSSKYVNWDGVHYTEAANEYVSSQILTGKYCDPPF 375

BLAST of Spg003383 vs. ExPASy TrEMBL
Match: A0A1S3C171 (GDSL esterase/lipase At1g54790-like OS=Cucumis melo OX=3656 GN=LOC103495312 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.5e-171
Identity = 302/376 (80.32%), Postives = 321/376 (85.37%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTIF+ GSS+  DYPAVFNFGDSNSDTGELLAGKGFSL LPYGETYF S SSGRF
Sbjct: 16  MLTMCSTIFRSGSSAGFDYPAVFNFGDSNSDTGELLAGKGFSLLLPYGETYFQSPSSGRF 75

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSFRKGCN+AAGGSTILP  AA ISPFSFGVQ
Sbjct: 76  CNGRLIIDFLMEATGLPYLRAYLDSVGRPSFRKGCNYAAGGSTILPGTAASISPFSFGVQ 135

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QF+HFK+RVLQLRA+GDKKI KFLPVE YFK GVYMFDIGQNDL + FYS  S DQAI
Sbjct: 136 INQFIHFKSRVLQLRAQGDKKIGKFLPVEKYFKDGVYMFDIGQNDLTAAFYSKASMDQAI 195

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEF+ GLQ+LY++GARNFWIHN+GPLGCLAQNIA FG  DPSKLD+ GCL SHNQA
Sbjct: 196 PTILTEFEIGLQKLYEQGARNFWIHNTGPLGCLAQNIATFG-SDPSKLDEFGCLTSHNQA 255

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           AKLFNSQLH LSKKLQ+QY DATVTYVDIY IK NLIANYSQLGFEQPIM CCG GGPPL
Sbjct: 256 AKLFNSQLHDLSKKLQSQYVDATVTYVDIYTIKFNLIANYSQLGFEQPIMTCCGFGGPPL 315

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 359
           NYDSR+SCGLTK LNGTVVTA GCDDSSKYVNWDG HYTEAANEYVSSQILTGKY DPPF
Sbjct: 316 NYDSRLSCGLTKTLNGTVVTANGCDDSSKYVNWDGVHYTEAANEYVSSQILTGKYCDPPF 375

BLAST of Spg003383 vs. ExPASy TrEMBL
Match: A0A6J1KIP9 (GDSL esterase/lipase At1g54790-like OS=Cucurbita maxima OX=3661 GN=LOC111493597 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 295/372 (79.30%), Postives = 319/372 (85.75%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTIF+ GSSSV  YPAVFNFGDSNSDTG+LLAGKGFSL LPYGETYF S SSGRF
Sbjct: 14  MLTMCSTIFRSGSSSVFHYPAVFNFGDSNSDTGDLLAGKGFSLPLPYGETYFQSPSSGRF 73

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLIIDFLM               G+PSFRKGCN+AAGGST+LPA AA +SPF+FGVQ
Sbjct: 74  CNGRLIIDFLMEAMGKPHLRAYLESVGRPSFRKGCNYAAGGSTVLPATAASVSPFAFGVQ 133

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QFLHFK RVL LRAKG+KK++K LPVE  FKKGVYMFDIGQNDL + FYS T  DQAI
Sbjct: 134 LNQFLHFKDRVLLLRAKGNKKVNKLLPVEKSFKKGVYMFDIGQNDLTAAFYSKTPLDQAI 193

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEFQ GLQRLY+ GAR+FWIHN+GPLGCLAQNIA FG  DPSKLD+ GCLISHNQA
Sbjct: 194 PTILTEFQTGLQRLYENGARHFWIHNTGPLGCLAQNIATFG-SDPSKLDEFGCLISHNQA 253

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           A LFNSQLHAL K LQ+QY DATVTYVDIYAIK NL+ANYSQLGFEQPIM CCG GGPPL
Sbjct: 254 ANLFNSQLHALCKTLQSQYVDATVTYVDIYAIKFNLVANYSQLGFEQPIMTCCGYGGPPL 313

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 357
           NYD+RISCGLTK L+G VVTAKGC+DSSKYVNWDGTHYTEAANEYVSSQILTGKY+DPPF
Sbjct: 314 NYDNRISCGLTKTLDGKVVTAKGCNDSSKYVNWDGTHYTEAANEYVSSQILTGKYSDPPF 373

BLAST of Spg003383 vs. ExPASy TrEMBL
Match: A0A6J1G3D1 (GDSL esterase/lipase At1g54790-like OS=Cucurbita moschata OX=3662 GN=LOC111450411 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.9e-166
Identity = 291/372 (78.23%), Postives = 318/372 (85.48%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           MLTMCSTIF+ GSSSV  YPAVFNFGDSNSDTGELLAGKGF L LPYGETYF S SSGRF
Sbjct: 14  MLTMCSTIFRSGSSSVFHYPAVFNFGDSNSDTGELLAGKGFRLPLPYGETYFQSPSSGRF 73

Query: 61  CNGRLIIDFLM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           CNGRLI+DFLM               G+PSFRKGCN+AAGGST+LPA AA +SPF+FGVQ
Sbjct: 74  CNGRLIVDFLMEAMGKPHLRAYLESVGRPSFRKGCNYAAGGSTVLPATAASVSPFAFGVQ 133

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQAI 180
           ++QFLHFK RVL+LRAKG+KK++K LPVE  FK GVYMFDIGQNDL + FYS T  DQAI
Sbjct: 134 LNQFLHFKDRVLRLRAKGNKKVNKLLPVEKSFKNGVYMFDIGQNDLTAAFYSKTPLDQAI 193

Query: 181 PTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQA 240
           PTILTEFQ G+QRLY+ GAR+FWIHN+GPLGCLAQNIA FG  DPSKLD+ GCLISHNQA
Sbjct: 194 PTILTEFQTGIQRLYENGARHFWIHNTGPLGCLAQNIATFGT-DPSKLDEFGCLISHNQA 253

Query: 241 AKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPL 300
           A LFNSQLHAL K LQ+QY DATVTYVDIYAIK NL+ANYSQLGFEQPIM CCG GGPPL
Sbjct: 254 ANLFNSQLHALCKTLQSQYADATVTYVDIYAIKFNLVANYSQLGFEQPIMTCCGYGGPPL 313

Query: 301 NYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPF 357
           NYD+RI+CGLTK L+G VVTAKGC+DSSKYVNWDGTHYTEAANEYVSSQILTGKY+DPPF
Sbjct: 314 NYDNRITCGLTKTLDGKVVTAKGCNDSSKYVNWDGTHYTEAANEYVSSQILTGKYSDPPF 373

BLAST of Spg003383 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 464.9 bits (1195), Expect = 5.7e-131
Identity = 228/366 (62.30%), Postives = 280/366 (76.50%), Query Frame = 0

Query: 10  QCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDF 69
           Q  +S   +YP+ FNFGDSNSDTG+L+AG G  L LP G+  F + SS RFC+GRL+IDF
Sbjct: 20  QISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKT-SSQRFCDGRLVIDF 79

Query: 70  LM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKA 129
           LM               G P+F+KGCNFAA GSTILPAN   +SPFSF +Q+SQF+ FK+
Sbjct: 80  LMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFKS 139

Query: 130 RVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPD--QAIPTILTEF 189
           R ++L +K  +K +K+LP  +Y+ KG+YM DIGQND+   FYS T      +IP+IL  F
Sbjct: 140 RAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETF 199

Query: 190 QAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQAAKLFNSQ 249
           +AGL+RLY+EG RN WIHN+GPLGCLAQNIA FG  D +KLD+ GC+ SHNQAAKLFN Q
Sbjct: 200 EAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGT-DSTKLDEFGCVSSHNQAAKLFNLQ 259

Query: 250 LHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPLNYDSRIS 309
           LHA+S K QAQY DA VTYVDI++IK NLIANYS+ GFE+P+MACCG GG PLNYDSRI+
Sbjct: 260 LHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGGAPLNYDSRIT 319

Query: 310 CGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPFSSNKLSF 359
           CG TK+L+G  VTAK C+DSS+Y+NWDG HYTEAANE+VSSQILTGKY+DPPF S+++ F
Sbjct: 320 CGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDPPF-SDQMPF 379

BLAST of Spg003383 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-130
Identity = 226/375 (60.27%), Postives = 282/375 (75.20%), Query Frame = 0

Query: 1   MLTMCSTIFQCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRF 60
           +  + S       S +L YPA+ NFGDSNSDTG L++    ++  PYG+TYFN   SGR+
Sbjct: 12  LFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFN-LPSGRY 71

Query: 61  CNGRLIIDFL---------------MGKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQ 120
           C+GRLI+DFL               +G P+F+KGCNFAA GSTILPAN   +SPFSF +Q
Sbjct: 72  CDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQ 131

Query: 121 VSQFLHFKARVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPD--Q 180
           +SQF+ FK+R ++L +K  +K +K+LP  +Y+ KG+YM DIGQND+   FYS T      
Sbjct: 132 ISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLA 191

Query: 181 AIPTILTEFQAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHN 240
           +IP+IL  F+AGL+RLY+EG RN WIHN+GPLGCLAQNIA FG  D +KLD+ GC+ SHN
Sbjct: 192 SIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGT-DSTKLDEFGCVSSHN 251

Query: 241 QAAKLFNSQLHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGP 300
           QAAKLFN QLHA+S K QAQY DA VTYVDI++IK NLIANYS+ GFE+P+MACCG GG 
Sbjct: 252 QAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGGA 311

Query: 301 PLNYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADP 359
           PLNYDSRI+CG TK+L+G  VTAK C+DSS+Y+NWDG HYTEAANE+VSSQILTGKY+DP
Sbjct: 312 PLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDP 371

BLAST of Spg003383 vs. TAIR 10
Match: AT1G54790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 452.2 bits (1162), Expect = 3.9e-127
Identity = 229/392 (58.42%), Postives = 281/392 (71.68%), Query Frame = 0

Query: 10  QCGSSSVLDYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDF 69
           Q  +S   +YP+ FNFGDSNSDTG+L+AG G  L LP G+  F + SS RFC+GRL+IDF
Sbjct: 20  QISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKT-SSQRFCDGRLVIDF 79

Query: 70  LM---------------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKA 129
           LM               G P+F+KGCNFAA GSTILPAN   +SPFSF +Q+SQF+ FK+
Sbjct: 80  LMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFKS 139

Query: 130 RVLQLRAKGDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPD--QAIPTILTEF 189
           R ++L +K  +K +K+LP  +Y+ KG+YM DIGQND+   FYS T      +IP+IL  F
Sbjct: 140 RAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETF 199

Query: 190 QAGLQRLYDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQAAKLFNSQ 249
           +AGL+RLY+EG RN WIHN+GPLGCLAQNIA FG  D +KLD+ GC+ SHNQAAKLFN Q
Sbjct: 200 EAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGT-DSTKLDEFGCVSSHNQAAKLFNLQ 259

Query: 250 LHALSKKLQAQYTDATVTYVDIYAIKLNLIANYSQ------------------------- 309
           LHA+S K QAQY DA VTYVDI++IK NLIANYS+                         
Sbjct: 260 LHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNHLENVGYNKILN 319

Query: 310 -LGFEQPIMACCGNGGPPLNYDSRISCGLTKMLNGTVVTAKGCDDSSKYVNWDGTHYTEA 359
            LGFE+P+MACCG GG PLNYDSRI+CG TK+L+G  VTAK C+DSS+Y+NWDG HYTEA
Sbjct: 320 VLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEA 379

BLAST of Spg003383 vs. TAIR 10
Match: AT3G05180.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.2e-115
Identity = 204/349 (58.45%), Postives = 254/349 (72.78%), Query Frame = 0

Query: 18  DYPAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDFLM------ 77
           D+PAVFNFGDSNSDTGEL +G GF  +  Y  T+F S +SGRFCNGRLI+DFLM      
Sbjct: 33  DFPAVFNFGDSNSDTGELSSGLGFLPQPSYEITFFRSPTSGRFCNGRLIVDFLMEAIDRP 92

Query: 78  ---------GKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKARVLQLRAK 137
                     + ++R+GCNFAA  STI  ANAA  SPF FGVQVSQF+ FK++VLQL  +
Sbjct: 93  YLRPYLDSISRQTYRRGCNFAAAASTIQKANAASYSPFGFGVQVSQFITFKSKVLQL-IQ 152

Query: 138 GDKKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQA---IPTILTEFQAGLQRL 197
            D+++ ++LP E +F  G+YMFDIGQND+   FY T + DQ    +P IL  FQ G++RL
Sbjct: 153 QDEELQRYLPSEYFFSNGLYMFDIGQNDIAGAFY-TKTVDQVLALVPIILDIFQDGIKRL 212

Query: 198 YDEGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQAAKLFNSQLHALSKK 257
           Y EGARN+WIHN+GPLGCLAQ +++FG+ D SKLD+ GC+  HNQAAKLFN QLH L KK
Sbjct: 213 YAEGARNYWIHNTGPLGCLAQVVSIFGE-DKSKLDEFGCVSDHNQAAKLFNLQLHGLFKK 272

Query: 258 LQAQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPLNYDSRISCGLTKML 317
           L  QY ++  TYVDI++IK +LI N+S+ GF+  IM CCG GGPPLNYD ++ CG T   
Sbjct: 273 LPQQYPNSRFTYVDIFSIKSDLILNHSKYGFDHSIMVCCGTGGPPLNYDDQVGCGKTARS 332

Query: 318 NGTVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPPFSSN 349
           NGT++TAK C DSSKYVNWDG HYTEAAN +V+  ILTGKY++   S N
Sbjct: 333 NGTIITAKPCYDSSKYVNWDGIHYTEAANRFVALHILTGKYSETASSLN 378

BLAST of Spg003383 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.4e-81
Identity = 156/343 (45.48%), Postives = 212/343 (61.81%), Query Frame = 0

Query: 20  PAVFNFGDSNSDTGELLAGKGFSLRLPYGETYFNSASSGRFCNGRLIIDFL--------- 79
           P +FNFGDSNSDTG L+AG G+ +  P G  +F   S+GR  +GRL+IDFL         
Sbjct: 39  PVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFR-RSTGRLSDGRLLIDFLCQSLNTSLL 98

Query: 80  ------MGKPSFRKGCNFAAGGSTILPANAAFISPFSFGVQVSQFLHFKARVLQLRAKGD 139
                 +G+  F+ G NFA  GS  LP N     PFS  +QV QF HFK+R L+L +  +
Sbjct: 99  RPYLDSLGRTRFQNGANFAIAGSPTLPKNV----PFSLNIQVKQFSHFKSRSLELASSSN 158

Query: 140 KKIDKFLPVENYFKKGVYMFDIGQNDLVSIFYSTTSPDQA---IPTILTEFQAGLQRLYD 199
                F+   N FK  +YM DIGQND+   F    S  Q    IP I+TE ++ ++RLYD
Sbjct: 159 SLKGMFIS-NNGFKNALYMIDIGQNDIARSFARGNSYSQTVKLIPQIITEIKSSIKRLYD 218

Query: 200 EGARNFWIHNSGPLGCLAQNIAVFGKGDPSKLDDLGCLISHNQAAKLFNSQLHALSKKLQ 259
           EG R FWIHN+GPLGCL Q +++    D   LD  GCL+S+N AA LFN  L  + ++L+
Sbjct: 219 EGGRRFWIHNTGPLGCLPQKLSMVKSKD---LDQHGCLVSYNSAATLFNQGLDHMCEELR 278

Query: 260 AQYTDATVTYVDIYAIKLNLIANYSQLGFEQPIMACCGNGGPPLNYDSRISCGLTKMLNG 319
            +  DAT+ Y+DIYAIK +LIAN +Q GF+ P+MACCG GG P NY+ +I+CG      G
Sbjct: 279 TELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMACCGYGGTPYNYNVKITCG----HKG 338

Query: 320 TVVTAKGCDDSSKYVNWDGTHYTEAANEYVSSQILTGKYADPP 345
           + V    C++ S++++WDG HYTE AN  V+ ++L+  Y+ PP
Sbjct: 339 SNV----CEEGSRFISWDGIHYTETANAIVAMKVLSMHYSKPP 364