Spg003374 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003374
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold4: 46411743 .. 46412690 (+)
RNA-Seq ExpressionSpg003374
SyntenySpg003374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATCCCAAGTAAGGTAAAAATTTTCTTTTACCATTTATGCTTGAATAGACTCCCAACTATAGACAATTTACTCAAGCGTGGGGTTGATGTCTTGAATGTTTGCTCCCTTTCTGGTTGTCAGGGGGAAACTGCACTGCACGTTTTCTGGCTTTGTAAATTTTCTAAGAATGTCATTAGCAATGCTGGTTTTGGTCCCCTTTTGAGTCAAGTACAGTCGGATTGTATTTTCCTGATTCTGAGAGATATTAGGGACAACTTGGATTGGGCTCGTTTTGAGTTCTTTGTAGTGTTGCTTTGGGCTTTGTGGGATTGCAGAAATCGAGTGAAAATGAAGGGGGAGAGGCTGTCGTTGGAGATTCCGACGTGGTCTACTAGTTTTCTGGATTCATTCCGGCGAGCTGTTTCGAATCGGGTGTCTGTGGTTTCTGATGGCCTTGGCCGTGAAGGGGTGAGGTGGGTAGCTCCGTCGGTTAGCTGGTATAAGCTTAACGTTGATGCAGCTTTCGACGGTGAGAGGTTGTTGGCTGGAGTTGGGCTGGTTGCTCGCAATCACAGAGGCGAGATTATGGCAGCATCAACAAGATCCCATGGTTTTGTTGGAAACTCTGATATGGCTGAAGCGTGGGCGGTGGTTGACGGTATTAAGCTCGCCGCTGAGTTGGGCCTCTACCCGGTTTTGATTGAGATTGATTCCAAGAGGGCCTTTGAGGCTATGCAGAGGGAGAAAGAAGACATTTATGATCTCTTTGTTCTGATCTCTGATGCAATTGATGCTTGGCCTTTGTCATGGGCTTTAAAATGTGTTTTTACCTATCGTGAAGGGAACCGCTTGGCCCACCATATGGCCAAGTTGGCGATGTGTGATCGTAGGGATTATTTTTGGCTTGAGGAAATTCTTGTTTGTGCTGAGAGTCTTTTGCTTTTAGACTCTATTTCGTTGTAA

mRNA sequence

ATGGCAATCCCAAGTAAGGTAAAAATTTTCTTTTACCATTTATGCTTGAATAGACTCCCAACTATAGACAATTTACTCAAGCGTGGGGTTGATGTCTTGAATGTTTGCTCCCTTTCTGGTTGTCAGGGGGAAACTGCACTGCACGTTTTCTGGCTTTGTAAATTTTCTAAGAATGTCATTAGCAATGCTGGTTTTGGTCCCCTTTTGAGTCAAGTACAGTCGGATTGTATTTTCCTGATTCTGAGAGATATTAGGGACAACTTGGATTGGGCTCGTTTTGAGTTCTTTGTAGTGTTGCTTTGGGCTTTGTGGGATTGCAGAAATCGAGTGAAAATGAAGGGGGAGAGGCTGTCGTTGGAGATTCCGACGTGGTCTACTAGTTTTCTGGATTCATTCCGGCGAGCTGTTTCGAATCGGGTGTCTGTGGTTTCTGATGGCCTTGGCCGTGAAGGGGTGAGGTGGGTAGCTCCGTCGGTTAGCTGGTATAAGCTTAACGTTGATGCAGCTTTCGACGGTGAGAGGTTGTTGGCTGGAGTTGGGCTGGTTGCTCGCAATCACAGAGGCGAGATTATGGCAGCATCAACAAGATCCCATGGTTTTGTTGGAAACTCTGATATGGCTGAAGCGTGGGCGGTGGTTGACGGTATTAAGCTCGCCGCTGAGTTGGGCCTCTACCCGGTTTTGATTGAGATTGATTCCAAGAGGGCCTTTGAGGCTATGCAGAGGGAGAAAGAAGACATTTATGATCTCTTTGTTCTGATCTCTGATGCAATTGATGCTTGGCCTTTGTCATGGGCTTTAAAATGTGTTTTTACCTATCGTGAAGGGAACCGCTTGGCCCACCATATGGCCAAGTTGGCGATGTGTGATCGTAGGGATTATTTTTGGCTTGAGGAAATTCTTGTTTGTGCTGAGAGTCTTTTGCTTTTAGACTCTATTTCGTTGTAA

Coding sequence (CDS)

ATGGCAATCCCAAGTAAGGTAAAAATTTTCTTTTACCATTTATGCTTGAATAGACTCCCAACTATAGACAATTTACTCAAGCGTGGGGTTGATGTCTTGAATGTTTGCTCCCTTTCTGGTTGTCAGGGGGAAACTGCACTGCACGTTTTCTGGCTTTGTAAATTTTCTAAGAATGTCATTAGCAATGCTGGTTTTGGTCCCCTTTTGAGTCAAGTACAGTCGGATTGTATTTTCCTGATTCTGAGAGATATTAGGGACAACTTGGATTGGGCTCGTTTTGAGTTCTTTGTAGTGTTGCTTTGGGCTTTGTGGGATTGCAGAAATCGAGTGAAAATGAAGGGGGAGAGGCTGTCGTTGGAGATTCCGACGTGGTCTACTAGTTTTCTGGATTCATTCCGGCGAGCTGTTTCGAATCGGGTGTCTGTGGTTTCTGATGGCCTTGGCCGTGAAGGGGTGAGGTGGGTAGCTCCGTCGGTTAGCTGGTATAAGCTTAACGTTGATGCAGCTTTCGACGGTGAGAGGTTGTTGGCTGGAGTTGGGCTGGTTGCTCGCAATCACAGAGGCGAGATTATGGCAGCATCAACAAGATCCCATGGTTTTGTTGGAAACTCTGATATGGCTGAAGCGTGGGCGGTGGTTGACGGTATTAAGCTCGCCGCTGAGTTGGGCCTCTACCCGGTTTTGATTGAGATTGATTCCAAGAGGGCCTTTGAGGCTATGCAGAGGGAGAAAGAAGACATTTATGATCTCTTTGTTCTGATCTCTGATGCAATTGATGCTTGGCCTTTGTCATGGGCTTTAAAATGTGTTTTTACCTATCGTGAAGGGAACCGCTTGGCCCACCATATGGCCAAGTTGGCGATGTGTGATCGTAGGGATTATTTTTGGCTTGAGGAAATTCTTGTTTGTGCTGAGAGTCTTTTGCTTTTAGACTCTATTTCGTTGTAA

Protein sequence

MAIPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVISNAGFGPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKGERLSLEIPTWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERLLAGVGLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRAFEAMQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYFWLEEILVCAESLLLLDSISL
Homology
BLAST of Spg003374 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 189.9 bits (481), Expect = 3.4e-44
Identity = 104/304 (34.21%), Postives = 162/304 (53.29%), Query Frame = 0

Query: 1    MAIPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVI 60
            M IP+K+K+F + LCL+RLPT  NL KRGV++ N C   G  GE ++H+FW+CKF++ + 
Sbjct: 842  MHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWICKFAEALW 901

Query: 61   SNAGFGPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKGE----- 120
             N+ FG L         FLILR+  ++L  A FE   V++W LW+ RN            
Sbjct: 902  INSKFGKLSP-------FLILRESHESLSKADFEELCVVIWGLWNQRNARAFNDSTKTVF 961

Query: 121  RLSLEIPTWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERL 180
            ++ +E+  W+  +   FR A SN   +         + W  P    YK+N DA+F     
Sbjct: 962  KIGMELVEWANKYAMEFREAKSN--PITGRVTNTAEILWQPPDEGIYKINTDASFLASDQ 1021

Query: 181  LAGVGLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKR 240
             AG+G++  N RG++MAA+T+    + + DMAEA A V+G++LA+E+G++P L       
Sbjct: 1022 HAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHPAL------- 1081

Query: 241  AFEAMQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYF 300
                     ED+ +   ++  A + W  S      F  REGN+ AH +A+ A+       
Sbjct: 1082 ---------EDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLHEFSI 1120

BLAST of Spg003374 vs. NCBI nr
Match: XP_006487889.1 (uncharacterized protein LOC102617714 [Citrus sinensis])

HSP 1 Score: 147.5 bits (371), Expect = 1.9e-31
Identity = 87/295 (29.49%), Postives = 142/295 (48.14%), Query Frame = 0

Query: 3    IPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVISN 62
            +P K+KIF +    N LPT  NL +R +    +C   G + E  +H    CK +K V  N
Sbjct: 927  LPEKIKIFMWRAVKNLLPTASNLWRRKIVGSPICRRCGVKNEDVIHALLDCKVAKKVWRN 986

Query: 63   AGFGPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKGERLSLEIP 122
            AG    + ++    +  +L +I+        E  VVL W +W  +N    + +R   ++ 
Sbjct: 987  AGCAEEIEKLVQQDLMAVLVEIQRKKGKKELELIVVLCWTIWYSQNLFIFENKREDSQLS 1046

Query: 123  -TWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERLLAGVGL 182
               + + +DSF+R       ++          W  P   W+K+NVDAA + +  +AG+G+
Sbjct: 1047 IARAEANMDSFKRIKKPFSKILEKQQRNRKQVWNPPPSGWFKINVDAAVNVKDQIAGLGV 1106

Query: 183  VARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRAFEAMQ 242
            + RN  GE++AA+ +   F  +S   EA AV+ GIK A   G  P++IE DS+   +   
Sbjct: 1107 IIRNSNGEVVAAAIQRSFFQLSSTHMEAEAVILGIKSAQRAGFSPMIIETDSQEVVDLTL 1166

Query: 243  REKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYFW 297
             +K  I +   +I+D  ++   S      +T RE N  AH +AK A+      FW
Sbjct: 1167 SKKVRIIETSWIIADIQESIQSSNQFSIQYTPRECNVTAHDLAKKALEFENSVFW 1221

BLAST of Spg003374 vs. NCBI nr
Match: XP_024046693.1 (uncharacterized protein LOC112101029 [Citrus clementina])

HSP 1 Score: 139.4 bits (350), Expect = 5.2e-29
Identity = 89/299 (29.77%), Postives = 145/299 (48.49%), Query Frame = 0

Query: 3   IPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVISN 62
           +P KVKIF +    N LPT+ NL +R V     C +  C+GE   H    C+ +K V   
Sbjct: 20  LPEKVKIFIWRAAQNLLPTVGNLWRRRVVKDYFCQICNCRGEDVFHALIECRAAKRVWKL 79

Query: 63  AGFGPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKG-ERLSLEI 122
             F   ++Q+    +  +L+D+         E  V + WA+W  RNR+  +G E      
Sbjct: 80  TEFYEDVTQLAPQDMLSVLQDLTVKRKKNELELVVAVCWAIWYSRNRLVFEGKEEYPKNS 139

Query: 123 PTWSTSFLDSFRR-AVSNRVSVVSDGLGR-EGVRWVAPSVSWYKLNVDAAFDGERLLAGV 182
              + + ++S+RR  V N  +  S G  R   + W  P    YK+NVDAA +     AG+
Sbjct: 140 VARAIAMVESYRRIKVPNEQN--SPGHSRISQLEWANPPKGCYKVNVDAAINVSNQKAGL 199

Query: 183 GLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRAFEA 242
           G+V RN  G+++AA+ +   + G+    EA AV+ GI+ A +  ++P++IE DSK   + 
Sbjct: 200 GVVIRNSVGKVVAAAVQGAPYRGDVACMEAEAVLFGIQSAQQAAVFPIIIESDSKEVVDL 259

Query: 243 MQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYFWLE 299
             ++K    ++   I++              +  R  N +AH +AK+A+      FWLE
Sbjct: 260 SLKKKISKTEIEWKITEIQANLENQNVFSLSYVPRSCNSIAHSIAKIALGFESQVFWLE 316

BLAST of Spg003374 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 134.0 bits (336), Expect = 2.2e-27
Identity = 87/302 (28.81%), Postives = 147/302 (48.68%), Query Frame = 0

Query: 1    MAIPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVI 60
            +AIP KVKIF +    + LPT +NL K+ V    +C    C  ET  H    C  ++ + 
Sbjct: 844  LAIPEKVKIFLWRAAHDLLPTAENLWKKKVLQEPMCQSCHCHVETVSHALVECNRARKIW 903

Query: 61   SNAGFGPLLSQV-QSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKGERLS- 120
              +     L  V + D ++++    R +      E    LLWA+W  RN+   +G++ + 
Sbjct: 904  RYSNLAEELRGVYRCDIVWMLQFWPRQHAKVEGAE-VAALLWAIWKARNKWLFEGKKENP 963

Query: 121  LEIPTWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERLLAG 180
            L +   + + ++SF++     +   + G      +W  P   W K+NVDAA D E  +AG
Sbjct: 964  LRVVANAEAIVESFKKIRQPEMVYKTKGNAERQKQWSPPPNGWQKVNVDAAVDVENQMAG 1023

Query: 181  VGLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRAFE 240
            +G+V R+  G   AA+ +S    G+  MAEA A+  G+K+A +  +   + E DS    +
Sbjct: 1024 LGVVVRDSDGNCRAAAIKSLRLPGSVAMAEATAMEWGLKVAEKAHITFGIFESDSLEVID 1083

Query: 241  AMQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYFWLE 300
             + ++   + ++  LISD  +        K   + R+ N  AH +AKLA+  +    WL+
Sbjct: 1084 LINKKSSSLTEIGWLISDIQENLQNFQNFKAQHSPRDCNYAAHSLAKLALQKKETVIWLD 1143

BLAST of Spg003374 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 133.7 bits (335), Expect = 2.9e-27
Identity = 86/301 (28.57%), Postives = 141/301 (46.84%), Query Frame = 0

Query: 1    MAIPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVI 60
            + +P K+KIF +    N LP+ +NL KR V     C       ET  H    CK ++ + 
Sbjct: 1080 LELPEKLKIFMWRASNNLLPSAENLWKRKVVEEPTCKRCKLSVETISHALLECKAARKIW 1139

Query: 61   SNAGFGPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKGERLSLE 120
              + F     +  S  IF  L+++   L  +  E  V L W+ W  RN+    G  L+  
Sbjct: 1140 LQSPFSAPRLEANSQDIFSTLQNMAKELRKSDLELMVALCWSAWYARNKCIFDGRELNPI 1199

Query: 121  I-PTWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERLLAGV 180
            I    + S L +F+R    + S +S  +  +   W+ P  + +K+NVDAAF+ + L AGV
Sbjct: 1200 ISAAKAESVLTAFQRVRKPQQSHISISIKEKQQEWLPPPQNVFKVNVDAAFNSKNLSAGV 1259

Query: 181  GLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRAFEA 240
            G V R+  G+I+AA    +   G++ +AEA AV+ G++LA    +  ++IE D     + 
Sbjct: 1260 GAVIRDSNGKIVAAGVNQNLLKGSASLAEAEAVLWGLQLARNADVSSLIIESDCLEVVQL 1319

Query: 241  MQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYFWLEE 300
            +   K    ++F  I    +   +   +      R  N  AH++AK+A+       WL  
Sbjct: 1320 VNNTKGSRSEIFWTILAIQNQMKIFQKVVVNHIPRHCNACAHYLAKIALGKISPCMWLGN 1379

BLAST of Spg003374 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.6e-44
Identity = 104/304 (34.21%), Postives = 162/304 (53.29%), Query Frame = 0

Query: 1    MAIPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVI 60
            M IP+K+K+F + LCL+RLPT  NL KRGV++ N C   G  GE ++H+FW+CKF++ + 
Sbjct: 842  MHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWICKFAEALW 901

Query: 61   SNAGFGPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKGE----- 120
             N+ FG L         FLILR+  ++L  A FE   V++W LW+ RN            
Sbjct: 902  INSKFGKLSP-------FLILRESHESLSKADFEELCVVIWGLWNQRNARAFNDSTKTVF 961

Query: 121  RLSLEIPTWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERL 180
            ++ +E+  W+  +   FR A SN   +         + W  P    YK+N DA+F     
Sbjct: 962  KIGMELVEWANKYAMEFREAKSN--PITGRVTNTAEILWQPPDEGIYKINTDASFLASDQ 1021

Query: 181  LAGVGLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKR 240
             AG+G++  N RG++MAA+T+    + + DMAEA A V+G++LA+E+G++P L       
Sbjct: 1022 HAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHPAL------- 1081

Query: 241  AFEAMQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYF 300
                     ED+ +   ++  A + W  S      F  REGN+ AH +A+ A+       
Sbjct: 1082 ---------EDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLHEFSI 1120

BLAST of Spg003374 vs. ExPASy TrEMBL
Match: A0A803PWX1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 1.6e-28
Identity = 94/315 (29.84%), Postives = 145/315 (46.03%), Query Frame = 0

Query: 1    MAIPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCS-LSGCQGETALHVFWLCKFSKNV 60
            + IP KVK F + +  + +PT   L  R + V   C   S    E   H  W C+ ++ V
Sbjct: 1553 LKIPPKVKHFVWKMAHSWIPTNSALAHRHIQVEPYCKRCSTGAYENVFHALWGCRVNREV 1612

Query: 61   ISNAGF-GPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKGER-L 120
             + AGF G L  Q + D +  ++R +        FEFF++L W LW  RN V   G +  
Sbjct: 1613 WNGAGFYGRLQRQGREDVLAFLMR-VSSLFTKDEFEFFLILSWNLWYIRNSVNHGGHKPQ 1672

Query: 121  SLEIPTWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERLLA 180
            +  I  W + FL  FR    N V+ V  G  RE  +W  P    +K+NVDA       LA
Sbjct: 1673 ASAILDWCSKFLHEFR---ENNVA-VQVGQRREEAKWTPPRRGSFKVNVDAGIKAGENLA 1732

Query: 181  GVGLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRAF 240
            G+  V R+H G +M A+TR      +    E  A++ GI+   +  L    +E D  +A 
Sbjct: 1733 GLSSVVRDHEGRVMVAATRFVEKKTSPLQGELQAILLGIQTGIQRNLPSFSVESDCLQAV 1792

Query: 241  EAMQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYFWL 300
              + +E+E   D+  LI+   +           F +RE N++AH +A  A+ ++    W+
Sbjct: 1793 NLVMKEEEGCRDVDGLIAQIKELLQHDRVAGVFFVFREANQVAHVLANDALINKASAMWV 1852

Query: 301  EEILVCAESLLLLDS 313
              +  CA   +  DS
Sbjct: 1853 GVVPFCASQAIRRDS 1862

BLAST of Spg003374 vs. ExPASy TrEMBL
Match: A0A2N9GET2 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25820 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 2.4e-27
Identity = 87/307 (28.34%), Postives = 152/307 (49.51%), Query Frame = 0

Query: 1   MAIPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVI 60
           + +P KV+ F +      LPT  NL +R V   ++C+    + E+ LH+ WLC  +K V 
Sbjct: 410 LQVPPKVRHFLWRASHESLPTRYNLHRRHVIDDSMCTSCNLEPESTLHILWLCSITKQVW 469

Query: 61  SNAGF-GPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKG--ERL 120
            +  +   L+    SD   L+L  ++  L     + F ++ W+LW  RN V++    E +
Sbjct: 470 QSVPWHRKLIRDTYSDFQELLLNCVQ-TLSPTELQLFAMMSWSLWYRRNCVRLNKPVEPV 529

Query: 121 SLEIPTWS---TSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGER 180
           +  +P        ++D   R + N++  V     R  V W  P++   K+N D A   E 
Sbjct: 530 ASLLPRTQELLQEYIDVQERLIQNQIQAVGQNQPRTTVSWKPPAMGRVKINYDGAVFNET 589

Query: 181 LLAGVGLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSK 240
             AG+G++ RN +G +MA+ ++   F  + +  EA A    ++L  +LG   V +E DS 
Sbjct: 590 GEAGIGVIIRNSQGAVMASLSQRILFPHSVEAVEATAARLAVQLVLDLGFKEVEVEGDSV 649

Query: 241 RAFEAMQREKEDIYDLF-VLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRD 300
              +A+  + E  Y L+  +I+D   A  L  ++  +   REGN++AH +AK A C++  
Sbjct: 650 NIVQALS-QTETSYTLYGHIINDTKLASQLLVSVHFLHVKREGNKVAHSLAKRARCNQPF 709

BLAST of Spg003374 vs. ExPASy TrEMBL
Match: A0A7N2LIH6 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 6.9e-27
Identity = 97/316 (30.70%), Postives = 151/316 (47.78%), Query Frame = 0

Query: 4    PSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVISNA 63
            P+K+K F +  C   LPT   L+ R +   + C   G + ET+ H  W C  +K      
Sbjct: 1753 PNKIKHFLWRACRGILPTKKCLVHRKIMKDDCCDFCG-ESETSGHCLWNCIVAKEAWKGL 1812

Query: 64   GF---GPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNRVKMKG-ERLSL 123
            GF    P       D ++L+L    D  DW   EFF ++ W+LW+ RN V+  G  +   
Sbjct: 1813 GFNIDNPEHVVEFLDVVWLLLESQGDK-DW---EFFAIVAWSLWNNRNNVRHGGVSKQGK 1872

Query: 124  EIPTWSTSFLDSFRRAVSNRVSVVSDGLGREGVRWVAPSVSWYKLNVDAAFDGERLLAGV 183
             I   +  + +  R A+  +  V       +  RW  P   WYK+NVDAA   E+   G+
Sbjct: 1873 SITEEARRYREEVRTALPAKGQVPKPMPKHK--RWSPPLQDWYKVNVDAAVFREQGTCGI 1932

Query: 184  GLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRAFEA 243
            G+V RN++G+IM A ++   F   +  AEA A   GI LA +LGL  +++E D++   +A
Sbjct: 1933 GVVIRNNKGQIMGAMSKKMLFPLRALEAEAKAAEAGILLAWDLGLKNIVVEGDAQLVIQA 1992

Query: 244  MQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAMCDRRDYFWLEE 303
            + +  +    +  +I  A     +  + K V T R  N  AH +A+ +        W+EE
Sbjct: 1993 L-KGVDAPTPIVKIIEGARRYLQMFCSWKAVHTNRRNNTAAHLLARESKNVNDYIIWVEE 2052

Query: 304  ILVCAESLLLLDSISL 316
               C E+ LL D I+L
Sbjct: 2053 SPPCIENQLLNDVIAL 2060

BLAST of Spg003374 vs. ExPASy TrEMBL
Match: A0A2U1KHJ0 (CCHC-type domain-containing protein OS=Artemisia annua OX=35608 GN=CTI12_AA602590 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 9.0e-27
Identity = 91/292 (31.16%), Postives = 142/292 (48.63%), Query Frame = 0

Query: 3    IPSKVKIFFYHLCLNRLPTIDNLLKRGVDVLNVCSLSGCQGETALHVFWLCKFSKNVISN 62
            +PSKVK+F +    N +PTIDNL  RG++  + C+  G   E  +HV + C  +K+V + 
Sbjct: 794  VPSKVKLFMWRAWNNYVPTIDNLKSRGLNPTSSCTHCGQTSENLVHVLFKCSVAKDVWNR 853

Query: 63   AGFGPLLSQVQSDCIFLILRDIRDNLDWARFEFFVVLLWALWDCRNR---VKMKGERLSL 122
              FG      Q    F     +      A +E F+++LW LW  RNR    ++ G   ++
Sbjct: 854  CNFG-CFYDTQGAITFQDFCQVILEKFLAEWETFMMILWGLWTRRNRHFHGQLNGREGNV 913

Query: 123  EIPTWSTSFLDSFRRAVSNRVSVVSDGLGREGVR---WVAPSVSWYKLNVDAAFDGERLL 182
            E+   + S L  + +A  N+   +S   G   +    W+ P V   K+N DAA+  E   
Sbjct: 914  EV--IAKSVLSDYHKA--NQRENISGNSGVHNIHAGMWLRPDVEHIKINCDAAWQKESGK 973

Query: 183  AGVGLVARNHRGEIMAASTRSHGFVGNSDMAEAWAVVDGIKLAAELGLYPVLIEIDSKRA 242
            AG+G VARN++GE++ +  RS  +  +   AEA AV   +      G   V+ E +S   
Sbjct: 974  AGLGFVARNYKGEVLFSGGRSECYASSPLEAEAKAVHWAMTRVICKGYSHVIFETNSLCL 1033

Query: 243  FEAMQREKEDIYDLFVLISDAIDAWPLSWALKCVFTYREGNRLAHHMAKLAM 289
             +A+Q  K  +  +  L S+ +            F  REGNRLAH +A LA+
Sbjct: 1034 VKALQ-NKVTLLQIASLFSEILSKLMAFNVCNWSFVKREGNRLAHSIASLAL 1079

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150918.13.4e-4434.21uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_006487889.11.9e-3129.49uncharacterized protein LOC102617714 [Citrus sinensis][more]
XP_024046693.15.2e-2929.77uncharacterized protein LOC112101029 [Citrus clementina][more]
XP_024037590.12.2e-2728.81uncharacterized protein LOC112097210 [Citrus clementina][more]
XP_024950112.12.9e-2728.57uncharacterized protein LOC112496847 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DAR41.6e-4434.21uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A803PWX11.6e-2829.84Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2N9GET22.4e-2728.34Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A7N2LIH66.9e-2730.70Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A2U1KHJ09.0e-2731.16CCHC-type domain-containing protein OS=Artemisia annua OX=35608 GN=CTI12_AA60259... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 3..58
e-value: 1.4E-10
score: 41.7
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 161..289
e-value: 3.2E-13
score: 51.9
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 165..287
e-value: 7.4E-20
score: 71.0
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 150..285
NoneNo IPR availablePANTHERPTHR47074:SF25SUBFAMILY NOT NAMEDcoord: 150..285
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 164..285
e-value: 1.49663E-23
score: 91.6068
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 161..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003374.1Spg003374.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity