Spg003351 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003351
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGDP-mannose-3',5'-epimerase
Locationscaffold4: 46091881 .. 46094906 (-)
RNA-Seq ExpressionSpg003351
SyntenySpg003351
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGCCATTCGATTCGACCCTTTTCTCTTTATCACCTTTCAACACTGCCCATTTCGTCTTCTTCCCTCTTCCACCTCTTACAACCTCTAATTTCCGCCATTTTTGTGGTAAATAACCCAACTCCGAGTTCGATTTTGTGGGTTCCTCTGGTTTTTTCTGTTCGTTACTGGAATTTCTCGTTGTTGATTGTTCGAATTTTTCTGTGTTTTCGTTCAGATTTAGGGTTTGTGTTGGTGGGTAGTTCTTGATTTCTTGTTATCTTTACTGGGAATGTTGTGTTTGGCTCGATTCGTTTGAAATTTGCATAGACTCTCTCTGTCTCCGTCTCTTTTTCAACATCCGTTGTGAAATTCACCTTCCCTTTTTGATGAATCTCCATTTTGGTGCAGAATGGGGAGCGCAGGGGAAACCAATTATGGCTCATACACCTATCAGGAGCTTGAAAGAGAGCCTTATTGGCCATCGGAGAAGCTGAGAATCTCCATCACCGGCGCCGGTGGCTTTATCGCCTCTCACATTGCAAGGCGTTTGAAGAGTGAAGGCCATTACATCATTGCTTCTGACTGGAAGAGGAATGAACACATGACAGAGGATATGTTCTGCCATGAATTCCATCTTGTTGATCTCAGAGTGATGGAAAATTGCTTGAAGGTGACTCAGAAAGTTGACCATGTCTTCAACCTGGCTGCTGATATGGGGGGTATGGGCTTCATCCAGTCCAACCACTCTGTTATCATGTATAATAACACCATGATCAGCTTCAACATGCTTGAAGCTGCAAGAATCAGCGGGGTGAAGAGGTTAGTTTTCAAGGAAATCTTTCTCAATTCATGGTTGTTAGGTCTGGTTTAAATCCTTATTAGTACAGGGCTGGTAGATGTTATTACCCTTTAGAGTTATGGCTGTTAGGGGAAGGGGGGGTGGGGTCAGGAGTAGCTAGAAATTGTTATAAGGGTAGCAGTTTGCATTCTAACATTTTTTTTTTATGTATATAATCTTTCTAATCTAAGACTGTCCCATTCTTTGTTTTTTGGCTGTGTCCCTACCACTGAGGAAGAAAGTATCCAACAGATAATTATGATTTTCTCTGTTGAGTGCAGGTTTTTCTATGCATCTAGTGCTTGCATCTACCCTGAATTTAAGCAATTGGAAACTAATGTGAGCTTGAAGGAGTCAGATGCCTGGCCTGCTGAGGTTCATATTTTCATCTGTTTTTAATCATTAGGAAATGGAACAACTGTTAGATGTGTGCTTACTAAAACCGTGTTAACGATTGTTTCATAGCCTCAAGATGCTTATGGTTTGGAGAAGCTTGCCACGGAGGAGTTGTGCAAGCATTATACGAAGGATTTCGGTATTGAGTGCCGAATTGGAAGGTTCCACAACATTTACGGTCCGTTTGGAACATGGAAAGGTAGATTCAAATGCAAGAAATGGTTATGTTTGATTTGAAGTTATCTCTAGACAATAACTATGTAAGTTCTGATGCTATCATTTTTTCATATAGGTGGAAGGGAAAAGGCTCCTGCTGCATTTTGCAGAAAGGCTCTCACTTCTGTAGATAAGTTTGAGATGTGGGGAGATGGTCTTCAGACAAGATCTTTCACCTTCATTGATGAATGTGTAGAAGGTGTTCTAAGGTAAGCAAGCTTAATTGCTGTAAATTTCATTATCTATGGTTCCATGTTTGAAACTTGTACTTTCTTGTAATCTTAATCATACCCCAAGGGTTCTCAATGTGGAAAGAGCTCGAGACATTAGGAGATACCCTTCAAGGACTTCGAGCCAAGGTGGCCGAAGCCTTCCTGGGATTAGTTGGGTGGGTTATGAGCATAATGCAAAGAGCAACATCTTTGATACCCCAGCAGAATGTCGAGTACTCTTTGGCAGATTCGTTTCAGCTCTAGCTTTAATGAATTAGGTTGAGCTAAAATGAAAATAATTGGTGTATATTTGAATGTAAGCTGTAAATTGATATGTTTGATTGTGAACTTTTTAGATTCATGAATTGGGGTGAATGCTCTATGATCAACATCTTAGTTGAGCGGAGAAACTGATACGATTGGTTGCCTCTCTTTTGGGTAGTTTTCTTTTTTAGATCATGTTATGGAATGCTGTGGGATGTTTATATTTCTCTGGCTGCAGCGGCTACCGCCATCTTTTTCCACCTTTTCGATTTATACAAACAACAAAAGATTGGCTCGCTCAGGACGAGATTAAAGTTGTCAAATTGAACTTCTGTTTGCAGATTGACTAAGTCAGACTTCCGAGAGCCAGTGAACATTGGAAGCGATGAGATGGTTAGCATGAACGAAATGGCTGAAATCGTTCTTAGCTTCGACAACAAAAAGCTCCCAATTCACCACATCCCTGGTCCCGAGGGTGTCCGTGGTCGTAACTCAGACAACACACTCATTAAAGAGAAACTTGGGTGGGCTCCCACAATGAAATTGAAGGTAAATTTAGATAAAGTAGCCATTTCTTTTCACCTCGACGACCAAAAGTTTATTCGAAACTTCTGTCGCCTCTGCTTTCCTGTGACTCTCTTGCTTATCAGTCCTTGCATATTGTATGTTCTACCTGTGTAGGATGGTTTGAGAATTACCTACTTCTGGATCAAGGAGCAGATTGAAAAAGAGAAGTCTCAAGGCATTGACCTGTCAGTTTATGGATCATCAAAGGTTGTTGGTACCCAAGCCCCGGTTCAACTCGGGTCACTCCGTGCTGCTGATGGTAAAGAGTAATTAGTTAGGACCCGGATAATCTGGTTGGGTGGTATCGGTACAACCCGAGCTTTACTAGATGTTGTTATCAAAAGTTGTTATTAGTATCTACTACATACTCTGTAGTATTTTGATGTTACTGGATACTTTTATATGCCTACTGTTCTATTCAATTTTATAAGCCCTTTCAACCCAGTCTGTTTCCTTAGAAGGAAATATTGTGGTTTCTTTAGCAGGTTCTTGTTCTTGTCTAATTTGAAATAAATTTTCCAATCTAATTTTTTTGATAATGCCAGTTTATTT

mRNA sequence

ATGGGGAGCGCAGGGGAAACCAATTATGGCTCATACACCTATCAGGAGCTTGAAAGAGAGCCTTATTGGCCATCGGAGAAGCTGAGAATCTCCATCACCGGCGCCGGTGGCTTTATCGCCTCTCACATTGCAAGGCGTTTGAAGAGTGAAGGCCATTACATCATTGCTTCTGACTGGAAGAGGAATGAACACATGACAGAGGATATGTTCTGCCATGAATTCCATCTTGTTGATCTCAGAGTGATGGAAAATTGCTTGAAGGTGACTCAGAAAGTTGACCATGTCTTCAACCTGGCTGCTGATATGGGGGGTATGGGCTTCATCCAGTCCAACCACTCTGTTATCATGTATAATAACACCATGATCAGCTTCAACATGCTTGAAGCTGCAAGAATCAGCGGGGTGAAGAGGTTTTTCTATGCATCTAGTGCTTGCATCTACCCTGAATTTAAGCAATTGGAAACTAATGTGAGCTTGAAGGAGTCAGATGCCTGGCCTGCTGAGCCTCAAGATGCTTATGGTTTGGAGAAGCTTGCCACGGAGGAGTTGTGCAAGCATTATACGAAGGATTTCGGTATTGAGTGCCGAATTGGAAGGTTCCACAACATTTACGGTGGAAGGGAAAAGGCTCCTGCTGCATTTTGCAGAAAGGCTCTCACTTCTGTAGATAAGTTTGAGATGTGGGGAGATGGTCTTCAGACAAGATCTTTCACCTTCATTGATGAATGTGTAGAAGGGTTCTCAATGTGGAAAGAGCTCGAGACATTAGGAGATACCCTTCAAGGACTTCGAGCCAAGGTGGCCGAAGCCTTCCTGGGATTAGTTGGGTGGGTTATGAGCATAATGCAAAGAGCAACATCTTTGATACCCCAGCAGAATGTCGAGTACTCTTTGGCAGATTCATTGACTAAGTCAGACTTCCGAGAGCCAGTGAACATTGGAAGCGATGAGATGGTTAGCATGAACGAAATGGCTGAAATCGTTCTTAGCTTCGACAACAAAAAGCTCCCAATTCACCACATCCCTGGTCCCGAGGGTGTCCGTGGTCGTAACTCAGACAACACACTCATTAAAGAGAAACTTGGGTGGGCTCCCACAATGAAATTGAAGGATGGTTTGAGAATTACCTACTTCTGGATCAAGGAGCAGATTGAAAAAGAGAAGTCTCAAGGCATTGACCTGTCAGTTTATGGATCATCAAAGGTTGTTGGTACCCAAGCCCCGGTTCAACTCGGGTCACTCCGTGCTGCTGATGGTAAAGAGTAA

Coding sequence (CDS)

ATGGGGAGCGCAGGGGAAACCAATTATGGCTCATACACCTATCAGGAGCTTGAAAGAGAGCCTTATTGGCCATCGGAGAAGCTGAGAATCTCCATCACCGGCGCCGGTGGCTTTATCGCCTCTCACATTGCAAGGCGTTTGAAGAGTGAAGGCCATTACATCATTGCTTCTGACTGGAAGAGGAATGAACACATGACAGAGGATATGTTCTGCCATGAATTCCATCTTGTTGATCTCAGAGTGATGGAAAATTGCTTGAAGGTGACTCAGAAAGTTGACCATGTCTTCAACCTGGCTGCTGATATGGGGGGTATGGGCTTCATCCAGTCCAACCACTCTGTTATCATGTATAATAACACCATGATCAGCTTCAACATGCTTGAAGCTGCAAGAATCAGCGGGGTGAAGAGGTTTTTCTATGCATCTAGTGCTTGCATCTACCCTGAATTTAAGCAATTGGAAACTAATGTGAGCTTGAAGGAGTCAGATGCCTGGCCTGCTGAGCCTCAAGATGCTTATGGTTTGGAGAAGCTTGCCACGGAGGAGTTGTGCAAGCATTATACGAAGGATTTCGGTATTGAGTGCCGAATTGGAAGGTTCCACAACATTTACGGTGGAAGGGAAAAGGCTCCTGCTGCATTTTGCAGAAAGGCTCTCACTTCTGTAGATAAGTTTGAGATGTGGGGAGATGGTCTTCAGACAAGATCTTTCACCTTCATTGATGAATGTGTAGAAGGGTTCTCAATGTGGAAAGAGCTCGAGACATTAGGAGATACCCTTCAAGGACTTCGAGCCAAGGTGGCCGAAGCCTTCCTGGGATTAGTTGGGTGGGTTATGAGCATAATGCAAAGAGCAACATCTTTGATACCCCAGCAGAATGTCGAGTACTCTTTGGCAGATTCATTGACTAAGTCAGACTTCCGAGAGCCAGTGAACATTGGAAGCGATGAGATGGTTAGCATGAACGAAATGGCTGAAATCGTTCTTAGCTTCGACAACAAAAAGCTCCCAATTCACCACATCCCTGGTCCCGAGGGTGTCCGTGGTCGTAACTCAGACAACACACTCATTAAAGAGAAACTTGGGTGGGCTCCCACAATGAAATTGAAGGATGGTTTGAGAATTACCTACTTCTGGATCAAGGAGCAGATTGAAAAAGAGAAGTCTCAAGGCATTGACCTGTCAGTTTATGGATCATCAAAGGTTGTTGGTACCCAAGCCCCGGTTCAACTCGGGTCACTCCGTGCTGCTGATGGTAAAGAGTAA

Protein sequence

MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWKRNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLATEELCKHYTKDFGIECRIGRFHNIYGGREKAPAAFCRKALTSVDKFEMWGDGLQTRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQNVEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSDNTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGSLRAADGKE
Homology
BLAST of Spg003351 vs. NCBI nr
Match: XP_022946492.1 (GDP-mannose 3,5-epimerase 2 [Cucurbita moschata] >XP_022999407.1 GDP-mannose 3,5-epimerase 2 [Cucurbita maxima] >XP_023547074.1 GDP-mannose 3,5-epimerase 2 [Cucurbita pepo subsp. pepo] >KAG7030304.1 GDP-mannose 3,5-epimerase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 717.2 bits (1850), Expect = 8.1e-203
Identity = 362/428 (84.58%), Postives = 367/428 (85.75%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGE NYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 1   MGSAGEANYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVMENCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCHEFHLVDLRVMENCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 121 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECR+GRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 181 EELCKHYTKDFGIECRVGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 240

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 241 TRSFTFIDECVEGV---------------LR----------------------------- 300

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD
Sbjct: 301 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLS+YGSSKVVGTQAPVQLGS
Sbjct: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSLYGSSKVVGTQAPVQLGS 376

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 421 LRAADGKE 376

BLAST of Spg003351 vs. NCBI nr
Match: XP_038890351.1 (GDP-mannose 3,5-epimerase 2 isoform X2 [Benincasa hispida] >XP_038890352.1 GDP-mannose 3,5-epimerase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 714.9 bits (1844), Expect = 4.0e-202
Identity = 360/428 (84.11%), Postives = 367/428 (85.75%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGS GETNYGSYTY+ELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 1   MGSTGETNYGSYTYEELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVM+NC+KVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCHEFHLVDLRVMDNCMKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 121 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECRIGRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 181 EELCKHYTKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 240

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 241 TRSFTFIDECVEGV---------------LR----------------------------- 300

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD
Sbjct: 301 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDL+VYGSSKVVGTQAPVQLGS
Sbjct: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLTVYGSSKVVGTQAPVQLGS 376

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 421 LRAADGKE 376

BLAST of Spg003351 vs. NCBI nr
Match: XP_038890350.1 (GDP-mannose 3,5-epimerase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 714.9 bits (1844), Expect = 4.0e-202
Identity = 360/428 (84.11%), Postives = 367/428 (85.75%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGS GETNYGSYTY+ELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 7   MGSTGETNYGSYTYEELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 66

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVM+NC+KVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 67  KNEHMTEDMFCHEFHLVDLRVMDNCMKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 126

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 127 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 186

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECRIGRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 187 EELCKHYTKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 246

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 247 TRSFTFIDECVEGV---------------LR----------------------------- 306

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD
Sbjct: 307 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 366

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDL+VYGSSKVVGTQAPVQLGS
Sbjct: 367 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLTVYGSSKVVGTQAPVQLGS 382

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 427 LRAADGKE 382

BLAST of Spg003351 vs. NCBI nr
Match: XP_022143153.1 (GDP-mannose 3,5-epimerase 2 [Momordica charantia] >XP_022143154.1 GDP-mannose 3,5-epimerase 2 [Momordica charantia])

HSP 1 Score: 709.5 bits (1830), Expect = 1.7e-200
Identity = 358/428 (83.64%), Postives = 366/428 (85.51%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHY+IASDWK
Sbjct: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYVIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFC EFHLVDLRVM+NCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCDEFHLVDLRVMDNCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKE+DAWPAEPQDAYGLEKLAT
Sbjct: 121 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKEADAWPAEPQDAYGLEKLAT 180

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECRIGRFHNIY       GGREKAPAAFCRKA+TSVDKFEMWGDGLQ
Sbjct: 181 EELCKHYTKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKAITSVDKFEMWGDGLQ 240

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 241 TRSFTFIDECVEGV---------------LR----------------------------- 300

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPI HIPGPEGVRGRNSD
Sbjct: 301 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIQHIPGPEGVRGRNSD 360

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEK+QGIDLSVYGSSKVVGTQAPVQLGS
Sbjct: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKTQGIDLSVYGSSKVVGTQAPVQLGS 376

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 421 LRAADGKE 376

BLAST of Spg003351 vs. NCBI nr
Match: TYK18415.1 (GDP-mannose 3,5-epimerase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 707.2 bits (1824), Expect = 8.4e-200
Identity = 358/428 (83.64%), Postives = 364/428 (85.05%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGET YGSYTYQELERE YWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 34  MGSAGETTYGSYTYQELEREAYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 93

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVM+NCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 94  KNEHMTEDMFCHEFHLVDLRVMDNCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 153

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 154 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 213

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECRIGRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 214 EELCKHYTKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 273

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 274 TRSFTFIDECVEGV---------------LR----------------------------- 333

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNK LPIHHIPGPEGVRGRNSD
Sbjct: 334 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKNLPIHHIPGPEGVRGRNSD 393

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITY WIKEQIEKEKS+GIDL+VYGSSKVVGTQAPVQLGS
Sbjct: 394 NTLIKEKLGWAPTMKLKDGLRITYMWIKEQIEKEKSKGIDLTVYGSSKVVGTQAPVQLGS 409

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 454 LRAADGKE 409

BLAST of Spg003351 vs. ExPASy Swiss-Prot
Match: A2Z7B3 (GDP-mannose 3,5-epimerase 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_032456 PE=2 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 3.0e-192
Identity = 339/430 (78.84%), Postives = 355/430 (82.56%), Query Frame = 0

Query: 1   MGSAGE--TNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASD 60
           MGS+ +  T YG YTY ELERE YWPSEKLRISITGAGGFI SHIARRLKSEGHYIIASD
Sbjct: 1   MGSSEKNGTAYGEYTYAELEREQYWPSEKLRISITGAGGFIGSHIARRLKSEGHYIIASD 60

Query: 61  WKRNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYN 120
           WK+NEHMTEDMFCHEFHLVDLRVM+NCLKVT  VDHVFNLAADMGGMGFIQSNHSVIMYN
Sbjct: 61  WKKNEHMTEDMFCHEFHLVDLRVMDNCLKVTNSVDHVFNLAADMGGMGFIQSNHSVIMYN 120

Query: 121 NTMISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKL 180
           NTMISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKL
Sbjct: 121 NTMISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKL 180

Query: 181 ATEELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDG 240
           ATEELCKHYTKDFGIECR+GRFHNIY       GGREKAPAAFCRKA TS D+FEMWGDG
Sbjct: 181 ATEELCKHYTKDFGIECRVGRFHNIYGPFGTWKGGREKAPAAFCRKAQTSTDRFEMWGDG 240

Query: 241 LQTRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQ 300
           LQTRSFTFIDECVEG                LR                           
Sbjct: 241 LQTRSFTFIDECVEGV---------------LR--------------------------- 300

Query: 301 QNVEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRN 360
                     LTKSDFREPVNIGSDEMVSMNEMAEI+LSF++++LPIHHIPGPEGVRGRN
Sbjct: 301 ----------LTKSDFREPVNIGSDEMVSMNEMAEIILSFEDRELPIHHIPGPEGVRGRN 360

Query: 361 SDNTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQL 420
           SDNTLIKEKLGWAPTMKLKDGLR TYFWIKEQIEKEK+QG+D++ YGSSKVV TQAPVQL
Sbjct: 361 SDNTLIKEKLGWAPTMKLKDGLRFTYFWIKEQIEKEKTQGVDIAGYGSSKVVSTQAPVQL 378

Query: 421 GSLRAADGKE 422
           GSLRAADGKE
Sbjct: 421 GSLRAADGKE 378

BLAST of Spg003351 vs. ExPASy Swiss-Prot
Match: A3C4S4 (GDP-mannose 3,5-epimerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GME-1 PE=1 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 5.1e-192
Identity = 339/430 (78.84%), Postives = 355/430 (82.56%), Query Frame = 0

Query: 1   MGSAGE--TNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASD 60
           MGS+ +  T YG YTY ELERE YWPSEKLRISITGAGGFI SHIARRLKSEGHYIIASD
Sbjct: 1   MGSSEKNGTAYGEYTYAELEREQYWPSEKLRISITGAGGFIGSHIARRLKSEGHYIIASD 60

Query: 61  WKRNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYN 120
           WK+NEHMTEDMFCHEFHLVDLRVM+NCLKVT  VDHVFNLAADMGGMGFIQSNHSVIMYN
Sbjct: 61  WKKNEHMTEDMFCHEFHLVDLRVMDNCLKVTNGVDHVFNLAADMGGMGFIQSNHSVIMYN 120

Query: 121 NTMISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKL 180
           NTMISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKL
Sbjct: 121 NTMISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKL 180

Query: 181 ATEELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDG 240
           ATEELCKHYTKDFGIECR+GRFHNIY       GGREKAPAAFCRKA TS D+FEMWGDG
Sbjct: 181 ATEELCKHYTKDFGIECRVGRFHNIYGPFGTWKGGREKAPAAFCRKAQTSTDRFEMWGDG 240

Query: 241 LQTRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQ 300
           LQTRSFTFIDECVEG                LR                           
Sbjct: 241 LQTRSFTFIDECVEGV---------------LR--------------------------- 300

Query: 301 QNVEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRN 360
                     LTKSDFREPVNIGSDEMVSMNEMAEI+LSF++++LPIHHIPGPEGVRGRN
Sbjct: 301 ----------LTKSDFREPVNIGSDEMVSMNEMAEIILSFEDRELPIHHIPGPEGVRGRN 360

Query: 361 SDNTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQL 420
           SDNTLIKEKLGWAPTMKLKDGLR TYFWIKEQIEKEK+QG+D++ YGSSKVV TQAPVQL
Sbjct: 361 SDNTLIKEKLGWAPTMKLKDGLRFTYFWIKEQIEKEKTQGVDIAGYGSSKVVSTQAPVQL 378

Query: 421 GSLRAADGKE 422
           GSLRAADGKE
Sbjct: 421 GSLRAADGKE 378

BLAST of Spg003351 vs. ExPASy Swiss-Prot
Match: Q2R1V8 (GDP-mannose 3,5-epimerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GME-2 PE=2 SV=2)

HSP 1 Score: 667.2 bits (1720), Expect = 1.3e-190
Identity = 335/417 (80.34%), Postives = 349/417 (83.69%), Query Frame = 0

Query: 12  YTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWKRNEHMTEDMFC 71
           YTY ELE+EPYWP EKLRISITGAGGFIASHIARRLKSEGHYIIASDWK+NEHMTEDMFC
Sbjct: 7   YTYVELEKEPYWPFEKLRISITGAGGFIASHIARRLKSEGHYIIASDWKKNEHMTEDMFC 66

Query: 72  HEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAAR 131
           HEFHLVDLRVM+NCLKVT  VDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAAR
Sbjct: 67  HEFHLVDLRVMDNCLKVTTGVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAAR 126

Query: 132 ISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLATEELCKHYTKDF 191
           I+GVKRFFYASSACIYPEFKQL+T VSLKESDAWPAEPQDAYGLEKLATEELCKHYTKDF
Sbjct: 127 INGVKRFFYASSACIYPEFKQLDTVVSLKESDAWPAEPQDAYGLEKLATEELCKHYTKDF 186

Query: 192 GIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQTRSFTFIDECV 251
           GIECR+GRFHNIY       GGREKAPAAFCRKALTS D+FEMWGDGLQTRSFTFIDECV
Sbjct: 187 GIECRVGRFHNIYGPFGTWKGGREKAPAAFCRKALTSTDRFEMWGDGLQTRSFTFIDECV 246

Query: 252 EGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQNVEYSLADSLTK 311
           EG                LR                                     LTK
Sbjct: 247 EGV---------------LR-------------------------------------LTK 306

Query: 312 SDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSDNTLIKEKLGWA 371
           SDFREPVNIGSDEMVSMNEMAEIVLSF+NK+LPIHHIPGPEGVRGRNSDNTLIKEKLGWA
Sbjct: 307 SDFREPVNIGSDEMVSMNEMAEIVLSFENKQLPIHHIPGPEGVRGRNSDNTLIKEKLGWA 366

Query: 372 PTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGSLRAADGKE 422
           PTM+LKDGLRITYFWIKEQ+EKEK++G+DLS YGSSKVV TQAPVQLGSLRAADGKE
Sbjct: 367 PTMRLKDGLRITYFWIKEQLEKEKAEGVDLSAYGSSKVVQTQAPVQLGSLRAADGKE 371

BLAST of Spg003351 vs. ExPASy Swiss-Prot
Match: Q93VR3 (GDP-mannose 3,5-epimerase OS=Arabidopsis thaliana OX=3702 GN=At5g28840 PE=1 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 4.1e-189
Identity = 333/429 (77.62%), Postives = 353/429 (82.28%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MG+   T+YG+YTY+ELERE YWPSE L+ISITGAGGFIASHIARRLK EGHY+IASDWK
Sbjct: 1   MGTTNGTDYGAYTYKELEREQYWPSENLKISITGAGGFIASHIARRLKHEGHYVIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFC EFHLVDLRVMENCLKVT+ VDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCDEFHLVDLRVMENCLKVTEGVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLE-TNVSLKESDAWPAEPQDAYGLEKLA 180
           MISFNM+EAARI+G+KRFFYASSACIYPEFKQLE TNVSLKESDAWPAEPQDAYGLEKLA
Sbjct: 121 MISFNMIEAARINGIKRFFYASSACIYPEFKQLETTNVSLKESDAWPAEPQDAYGLEKLA 180

Query: 181 TEELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGL 240
           TEELCKHY KDFGIECRIGRFHNIY       GGREKAPAAFCRKA TS D+FEMWGDGL
Sbjct: 181 TEELCKHYNKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKAQTSTDRFEMWGDGL 240

Query: 241 QTRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQ 300
           QTRSFTFIDECVEG                LR                            
Sbjct: 241 QTRSFTFIDECVEGV---------------LR---------------------------- 300

Query: 301 NVEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNS 360
                    LTKSDFREPVNIGSDEMVSMNEMAE+VLSF+ KKLPIHHIPGPEGVRGRNS
Sbjct: 301 ---------LTKSDFREPVNIGSDEMVSMNEMAEMVLSFEEKKLPIHHIPGPEGVRGRNS 360

Query: 361 DNTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLG 420
           DN LIKEKLGWAP M+LK+GLRITYFWIKEQIEKEK++G D+S+YGSSKVVGTQAPVQLG
Sbjct: 361 DNNLIKEKLGWAPNMRLKEGLRITYFWIKEQIEKEKAKGSDVSLYGSSKVVGTQAPVQLG 377

Query: 421 SLRAADGKE 422
           SLRAADGKE
Sbjct: 421 SLRAADGKE 377

BLAST of Spg003351 vs. ExPASy Swiss-Prot
Match: Q6GMI9 (UDP-glucuronic acid decarboxylase 1 OS=Danio rerio OX=7955 GN=uxs1 PE=2 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 1.0e-19
Identity = 90/375 (24.00%), Postives = 153/375 (40.80%), Query Frame = 0

Query: 26  EKLRISITGAGGFIASHIARRLKSEGHYIIASD--WKRNEHMTEDMFCHE-FHLVDLRVM 85
           ++ RI ITG  GF+ SH+  +L  +GH +   D  +   +   E    HE F L++  V+
Sbjct: 85  DRKRILITGGAGFVGSHLTDKLMMDGHEVTVVDNFFTGRKRNVEHWIGHENFELINHDVV 144

Query: 86  ENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAARISGVKRFFYAS 145
           E    +  +VD +++LA+      ++  N    +  NT+ + NML  A+  G  R   AS
Sbjct: 145 E---PLYIEVDQIYHLASPASPPNYMY-NPIKTLKTNTIGTLNMLGLAKRVGA-RLLLAS 204

Query: 146 SACIYPEFKQLETNVSLKESDAW----PAEPQDAYGLEKLATEELCKHYTKDFGIECRIG 205
           ++ +Y      +  V  +  D W    P  P+  Y   K   E +C  Y K  G+E R+ 
Sbjct: 205 TSEVYG-----DPEVHPQNEDYWGHVNPIGPRACYDEGKRVAETMCYAYMKQEGVEVRVA 264

Query: 206 RFHNIYGGR-----EKAPAAFCRKALTSVDKFEMWGDGLQTRSFTFIDECVEGFSMWKEL 265
           R  N +G R      +  + F  +AL   +   ++G G QTR+F ++ + V G       
Sbjct: 265 RIFNTFGSRMHMNDGRVVSNFILQALQG-EALTVYGSGSQTRAFQYVSDLVNGLV----- 324

Query: 266 ETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQNVEYSLADSLTKSDFREPVNI 325
                                                          SL  S+   PVN+
Sbjct: 325 -----------------------------------------------SLMNSNISSPVNL 384

Query: 326 GSDEMVSMNEMAEIVLSFDNKKLPIHHIP-GPEGVRGRNSDNTLIKEKLGWAPTMKLKDG 385
           G+ E  ++ E A+++ S    +  I  +P   +  + R  D    K  LGW P + L++G
Sbjct: 385 GNPEEHTILEFAQLIKSLVASRSHIQFLPEAQDDPQRRRPDIRKAKLLLGWEPVVPLEEG 396

Query: 386 LRITYFWIKEQIEKE 388
           L  T  +   ++E +
Sbjct: 445 LNKTIQYFSRELEHQ 396

BLAST of Spg003351 vs. ExPASy TrEMBL
Match: A0A6J1KAT0 (GDP-mannose 3,5-epimerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111493789 PE=3 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.9e-203
Identity = 362/428 (84.58%), Postives = 367/428 (85.75%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGE NYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 1   MGSAGEANYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVMENCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCHEFHLVDLRVMENCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 121 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECR+GRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 181 EELCKHYTKDFGIECRVGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 240

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 241 TRSFTFIDECVEGV---------------LR----------------------------- 300

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD
Sbjct: 301 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLS+YGSSKVVGTQAPVQLGS
Sbjct: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSLYGSSKVVGTQAPVQLGS 376

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 421 LRAADGKE 376

BLAST of Spg003351 vs. ExPASy TrEMBL
Match: A0A6J1G3W3 (GDP-mannose 3,5-epimerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111450536 PE=3 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.9e-203
Identity = 362/428 (84.58%), Postives = 367/428 (85.75%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGE NYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 1   MGSAGEANYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVMENCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCHEFHLVDLRVMENCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 121 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECR+GRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 181 EELCKHYTKDFGIECRVGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 240

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 241 TRSFTFIDECVEGV---------------LR----------------------------- 300

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD
Sbjct: 301 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLS+YGSSKVVGTQAPVQLGS
Sbjct: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSLYGSSKVVGTQAPVQLGS 376

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 421 LRAADGKE 376

BLAST of Spg003351 vs. ExPASy TrEMBL
Match: A0A6J1CNH4 (GDP-mannose 3,5-epimerase 2 OS=Momordica charantia OX=3673 GN=LOC111013089 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 8.2e-201
Identity = 358/428 (83.64%), Postives = 366/428 (85.51%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHY+IASDWK
Sbjct: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYVIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFC EFHLVDLRVM+NCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCDEFHLVDLRVMDNCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKE+DAWPAEPQDAYGLEKLAT
Sbjct: 121 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKEADAWPAEPQDAYGLEKLAT 180

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECRIGRFHNIY       GGREKAPAAFCRKA+TSVDKFEMWGDGLQ
Sbjct: 181 EELCKHYTKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKAITSVDKFEMWGDGLQ 240

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 241 TRSFTFIDECVEGV---------------LR----------------------------- 300

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPI HIPGPEGVRGRNSD
Sbjct: 301 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIQHIPGPEGVRGRNSD 360

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEK+QGIDLSVYGSSKVVGTQAPVQLGS
Sbjct: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKTQGIDLSVYGSSKVVGTQAPVQLGS 376

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 421 LRAADGKE 376

BLAST of Spg003351 vs. ExPASy TrEMBL
Match: A0A1S3C2Q9 (GDP-mannose 3,5-epimerase 2 OS=Cucumis melo OX=3656 GN=LOC103495988 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.1e-200
Identity = 358/428 (83.64%), Postives = 364/428 (85.05%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGET YGSYTYQELERE YWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 1   MGSAGETTYGSYTYQELEREAYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVM+NCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCHEFHLVDLRVMDNCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 121 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECRIGRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 181 EELCKHYTKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 240

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 241 TRSFTFIDECVEGV---------------LR----------------------------- 300

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNK LPIHHIPGPEGVRGRNSD
Sbjct: 301 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKNLPIHHIPGPEGVRGRNSD 360

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITY WIKEQIEKEKS+GIDL+VYGSSKVVGTQAPVQLGS
Sbjct: 361 NTLIKEKLGWAPTMKLKDGLRITYMWIKEQIEKEKSKGIDLTVYGSSKVVGTQAPVQLGS 376

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 421 LRAADGKE 376

BLAST of Spg003351 vs. ExPASy TrEMBL
Match: A0A5D3D4E2 (GDP-mannose 3,5-epimerase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001400 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.1e-200
Identity = 358/428 (83.64%), Postives = 364/428 (85.05%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MGSAGET YGSYTYQELERE YWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK
Sbjct: 34  MGSAGETTYGSYTYQELEREAYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 93

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFCHEFHLVDLRVM+NCLKVT+KVDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 94  KNEHMTEDMFCHEFHLVDLRVMDNCLKVTEKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 153

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 180
           MISFNMLEAARI+GVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT
Sbjct: 154 MISFNMLEAARINGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLAT 213

Query: 181 EELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGLQ 240
           EELCKHYTKDFGIECRIGRFHNIY       GGREKAPAAFCRKALTSVDKFEMWGDGLQ
Sbjct: 214 EELCKHYTKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKALTSVDKFEMWGDGLQ 273

Query: 241 TRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQN 300
           TRSFTFIDECVEG                LR                             
Sbjct: 274 TRSFTFIDECVEGV---------------LR----------------------------- 333

Query: 301 VEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNSD 360
                   LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNK LPIHHIPGPEGVRGRNSD
Sbjct: 334 --------LTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKNLPIHHIPGPEGVRGRNSD 393

Query: 361 NTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLGS 420
           NTLIKEKLGWAPTMKLKDGLRITY WIKEQIEKEKS+GIDL+VYGSSKVVGTQAPVQLGS
Sbjct: 394 NTLIKEKLGWAPTMKLKDGLRITYMWIKEQIEKEKSKGIDLTVYGSSKVVGTQAPVQLGS 409

Query: 421 LRAADGKE 422
           LRAADGKE
Sbjct: 454 LRAADGKE 409

BLAST of Spg003351 vs. TAIR 10
Match: AT5G28840.1 (GDP-D-mannose 3',5'-epimerase )

HSP 1 Score: 662.1 bits (1707), Expect = 2.9e-190
Identity = 333/429 (77.62%), Postives = 353/429 (82.28%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MG+   T+YG+YTY+ELERE YWPSE L+ISITGAGGFIASHIARRLK EGHY+IASDWK
Sbjct: 1   MGTTNGTDYGAYTYKELEREQYWPSENLKISITGAGGFIASHIARRLKHEGHYVIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFC EFHLVDLRVMENCLKVT+ VDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCDEFHLVDLRVMENCLKVTEGVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLE-TNVSLKESDAWPAEPQDAYGLEKLA 180
           MISFNM+EAARI+G+KRFFYASSACIYPEFKQLE TNVSLKESDAWPAEPQDAYGLEKLA
Sbjct: 121 MISFNMIEAARINGIKRFFYASSACIYPEFKQLETTNVSLKESDAWPAEPQDAYGLEKLA 180

Query: 181 TEELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGL 240
           TEELCKHY KDFGIECRIGRFHNIY       GGREKAPAAFCRKA TS D+FEMWGDGL
Sbjct: 181 TEELCKHYNKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKAQTSTDRFEMWGDGL 240

Query: 241 QTRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQ 300
           QTRSFTFIDECVEG                LR                            
Sbjct: 241 QTRSFTFIDECVEGV---------------LR---------------------------- 300

Query: 301 NVEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNS 360
                    LTKSDFREPVNIGSDEMVSMNEMAE+VLSF+ KKLPIHHIPGPEGVRGRNS
Sbjct: 301 ---------LTKSDFREPVNIGSDEMVSMNEMAEMVLSFEEKKLPIHHIPGPEGVRGRNS 360

Query: 361 DNTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLG 420
           DN LIKEKLGWAP M+LK+GLRITYFWIKEQIEKEK++G D+S+YGSSKVVGTQAPVQLG
Sbjct: 361 DNNLIKEKLGWAPNMRLKEGLRITYFWIKEQIEKEKAKGSDVSLYGSSKVVGTQAPVQLG 377

Query: 421 SLRAADGKE 422
           SLRAADGKE
Sbjct: 421 SLRAADGKE 377

BLAST of Spg003351 vs. TAIR 10
Match: AT5G28840.2 (GDP-D-mannose 3',5'-epimerase )

HSP 1 Score: 662.1 bits (1707), Expect = 2.9e-190
Identity = 333/429 (77.62%), Postives = 353/429 (82.28%), Query Frame = 0

Query: 1   MGSAGETNYGSYTYQELEREPYWPSEKLRISITGAGGFIASHIARRLKSEGHYIIASDWK 60
           MG+   T+YG+YTY+ELERE YWPSE L+ISITGAGGFIASHIARRLK EGHY+IASDWK
Sbjct: 1   MGTTNGTDYGAYTYKELEREQYWPSENLKISITGAGGFIASHIARRLKHEGHYVIASDWK 60

Query: 61  RNEHMTEDMFCHEFHLVDLRVMENCLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120
           +NEHMTEDMFC EFHLVDLRVMENCLKVT+ VDHVFNLAADMGGMGFIQSNHSVIMYNNT
Sbjct: 61  KNEHMTEDMFCDEFHLVDLRVMENCLKVTEGVDHVFNLAADMGGMGFIQSNHSVIMYNNT 120

Query: 121 MISFNMLEAARISGVKRFFYASSACIYPEFKQLE-TNVSLKESDAWPAEPQDAYGLEKLA 180
           MISFNM+EAARI+G+KRFFYASSACIYPEFKQLE TNVSLKESDAWPAEPQDAYGLEKLA
Sbjct: 121 MISFNMIEAARINGIKRFFYASSACIYPEFKQLETTNVSLKESDAWPAEPQDAYGLEKLA 180

Query: 181 TEELCKHYTKDFGIECRIGRFHNIY-------GGREKAPAAFCRKALTSVDKFEMWGDGL 240
           TEELCKHY KDFGIECRIGRFHNIY       GGREKAPAAFCRKA TS D+FEMWGDGL
Sbjct: 181 TEELCKHYNKDFGIECRIGRFHNIYGPFGTWKGGREKAPAAFCRKAQTSTDRFEMWGDGL 240

Query: 241 QTRSFTFIDECVEGFSMWKELETLGDTLQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQ 300
           QTRSFTFIDECVEG                LR                            
Sbjct: 241 QTRSFTFIDECVEGV---------------LR---------------------------- 300

Query: 301 NVEYSLADSLTKSDFREPVNIGSDEMVSMNEMAEIVLSFDNKKLPIHHIPGPEGVRGRNS 360
                    LTKSDFREPVNIGSDEMVSMNEMAE+VLSF+ KKLPIHHIPGPEGVRGRNS
Sbjct: 301 ---------LTKSDFREPVNIGSDEMVSMNEMAEMVLSFEEKKLPIHHIPGPEGVRGRNS 360

Query: 361 DNTLIKEKLGWAPTMKLKDGLRITYFWIKEQIEKEKSQGIDLSVYGSSKVVGTQAPVQLG 420
           DN LIKEKLGWAP M+LK+GLRITYFWIKEQIEKEK++G D+S+YGSSKVVGTQAPVQLG
Sbjct: 361 DNNLIKEKLGWAPNMRLKEGLRITYFWIKEQIEKEKAKGSDVSLYGSSKVVGTQAPVQLG 377

Query: 421 SLRAADGKE 422
           SLRAADGKE
Sbjct: 421 SLRAADGKE 377

BLAST of Spg003351 vs. TAIR 10
Match: AT3G62830.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 7.0e-19
Identity = 90/373 (24.13%), Postives = 147/373 (39.41%), Query Frame = 0

Query: 28  LRISITGAGGFIASHIARRLKSEGHYIIASD---WKRNEHMTEDMFCHEFHLVDLRVMEN 87
           LR+ +TG  GF+ SH+  RL + G  +I  D     R E++        F ++   V+E 
Sbjct: 119 LRVVVTGGAGFVGSHLVDRLMARGDTVIVVDNFFTGRKENVMHHFSNPNFEMIRHDVVE- 178

Query: 88  CLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAARISGVKRFFYASSA 147
              +  +VD +++LA     + + + N    +  N + + NML  A+  G  RF   S++
Sbjct: 179 --PILLEVDQIYHLACPASPVHY-KFNPVKTIKTNVVGTLNMLGLAKRVGA-RFLLTSTS 238

Query: 148 CIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLATEELCKHYTKDFGIECRIGRFHNIY 207
            +Y +  Q    V     +  P   +  Y   K   E L   Y +   +E RI R  N Y
Sbjct: 239 EVYGDPLQ-HPQVETYWGNVNPIGVRSCYDEGKRTAETLTMDYHRGANVEVRIARIFNTY 298

Query: 208 GGR-----EKAPAAFCRKALTSVDKFEMWGDGLQTRSFTFIDECVEGFSMWKELETLGDT 267
           G R      +  + F  +AL   +   ++GDG QTRSF F+ + VEG     E E +G  
Sbjct: 299 GPRMCIDDGRVVSNFVAQALRK-EPLTVYGDGKQTRSFQFVSDLVEGLMRLMEGEHVG-- 358

Query: 268 LQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQNVEYSLADSLTKSDFREPVNIGSDEMV 327
                                                             P N+G+    
Sbjct: 359 --------------------------------------------------PFNLGNPGEF 418

Query: 328 SMNEMAEIVLSFDNKKLPIHHIPGPE-GVRGRNSDNTLIKEKLGWAPTMKLKDGLRITYF 387
           +M E+A++V    +    I   P  E     R  D T  KE LGW P + L+ GL +   
Sbjct: 419 TMLELAKVVQETIDPNANIEFRPNTEDDPHKRKPDITKAKELLGWEPKVSLRQGLPLMVK 432

Query: 388 WIKEQIEKEKSQG 392
             ++++  ++ +G
Sbjct: 479 DFRQRVFGDQKEG 432

BLAST of Spg003351 vs. TAIR 10
Match: AT3G62830.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 7.0e-19
Identity = 90/373 (24.13%), Postives = 147/373 (39.41%), Query Frame = 0

Query: 28  LRISITGAGGFIASHIARRLKSEGHYIIASD---WKRNEHMTEDMFCHEFHLVDLRVMEN 87
           LR+ +TG  GF+ SH+  RL + G  +I  D     R E++        F ++   V+E 
Sbjct: 119 LRVVVTGGAGFVGSHLVDRLMARGDTVIVVDNFFTGRKENVMHHFSNPNFEMIRHDVVE- 178

Query: 88  CLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAARISGVKRFFYASSA 147
              +  +VD +++LA     + + + N    +  N + + NML  A+  G  RF   S++
Sbjct: 179 --PILLEVDQIYHLACPASPVHY-KFNPVKTIKTNVVGTLNMLGLAKRVGA-RFLLTSTS 238

Query: 148 CIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLATEELCKHYTKDFGIECRIGRFHNIY 207
            +Y +  Q    V     +  P   +  Y   K   E L   Y +   +E RI R  N Y
Sbjct: 239 EVYGDPLQ-HPQVETYWGNVNPIGVRSCYDEGKRTAETLTMDYHRGANVEVRIARIFNTY 298

Query: 208 GGR-----EKAPAAFCRKALTSVDKFEMWGDGLQTRSFTFIDECVEGFSMWKELETLGDT 267
           G R      +  + F  +AL   +   ++GDG QTRSF F+ + VEG     E E +G  
Sbjct: 299 GPRMCIDDGRVVSNFVAQALRK-EPLTVYGDGKQTRSFQFVSDLVEGLMRLMEGEHVG-- 358

Query: 268 LQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQNVEYSLADSLTKSDFREPVNIGSDEMV 327
                                                             P N+G+    
Sbjct: 359 --------------------------------------------------PFNLGNPGEF 418

Query: 328 SMNEMAEIVLSFDNKKLPIHHIPGPE-GVRGRNSDNTLIKEKLGWAPTMKLKDGLRITYF 387
           +M E+A++V    +    I   P  E     R  D T  KE LGW P + L+ GL +   
Sbjct: 419 TMLELAKVVQETIDPNANIEFRPNTEDDPHKRKPDITKAKELLGWEPKVSLRQGLPLMVK 432

Query: 388 WIKEQIEKEKSQG 392
             ++++  ++ +G
Sbjct: 479 DFRQRVFGDQKEG 432

BLAST of Spg003351 vs. TAIR 10
Match: AT2G47650.1 (UDP-xylose synthase 4 )

HSP 1 Score: 91.7 bits (226), Expect = 1.6e-18
Identity = 94/382 (24.61%), Postives = 150/382 (39.27%), Query Frame = 0

Query: 28  LRISITGAGGFIASHIARRLKSEGHYIIASD---WKRNEHMTEDMFCHEFHLVDLRVMEN 87
           LR+ +TG  GF+ SH+  RL + G  +I  D     R E++        F ++   V+E 
Sbjct: 121 LRVVVTGGAGFVGSHLVDRLMARGDNVIVVDNFFTGRKENVMHHFNNPNFEMIRHDVVE- 180

Query: 88  CLKVTQKVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAARISGVKRFFYASSA 147
              +  +VD +++LA     + + + N    +  N + + NML  A+  G  RF   S++
Sbjct: 181 --PILLEVDQIYHLACPASPVHY-KFNPVKTIKTNVVGTLNMLGLAKRVGA-RFLLTSTS 240

Query: 148 CIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLATEELCKHYTKDFGIECRIGRFHNIY 207
            +Y +  Q    V     +  P   +  Y   K   E L   Y +   +E RI R  N Y
Sbjct: 241 EVYGDPLQ-HPQVETYWGNVNPIGVRSCYDEGKRTAETLTMDYHRGANVEVRIARIFNTY 300

Query: 208 GGR-----EKAPAAFCRKALTSVDKFEMWGDGLQTRSFTFIDECVEGFSMWKELETLGDT 267
           G R      +  + F  +AL   +   ++GDG QTRSF F+ + VEG     E E +G  
Sbjct: 301 GPRMCIDDGRVVSNFVAQALRK-EPLTVYGDGKQTRSFQFVSDLVEGLMRLMEGEHVG-- 360

Query: 268 LQGLRAKVAEAFLGLVGWVMSIMQRATSLIPQQNVEYSLADSLTKSDFREPVNIGSDEMV 327
                                                             P N+G+    
Sbjct: 361 --------------------------------------------------PFNLGNPGEF 420

Query: 328 SMNEMAEIVLSFDNKKLPIHHIPGPE-GVRGRNSDNTLIKEKLGWAPTMKLKDGLRITYF 387
           +M E+A++V    +    I   P  E     R  D T  KE LGW P + L+ GL +   
Sbjct: 421 TMLELAKVVQETIDPNAKIEFRPNTEDDPHKRKPDITKAKELLGWEPKVALRQGLPLMVK 441

Query: 388 WIKEQIEKEKSQGIDLSVYGSS 401
             ++++  ++ Q  D S   SS
Sbjct: 481 DFRQRVFGDQKQ--DSSTTSSS 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022946492.18.1e-20384.58GDP-mannose 3,5-epimerase 2 [Cucurbita moschata] >XP_022999407.1 GDP-mannose 3,5... [more]
XP_038890351.14.0e-20284.11GDP-mannose 3,5-epimerase 2 isoform X2 [Benincasa hispida] >XP_038890352.1 GDP-m... [more]
XP_038890350.14.0e-20284.11GDP-mannose 3,5-epimerase 2 isoform X1 [Benincasa hispida][more]
XP_022143153.11.7e-20083.64GDP-mannose 3,5-epimerase 2 [Momordica charantia] >XP_022143154.1 GDP-mannose 3,... [more]
TYK18415.18.4e-20083.64GDP-mannose 3,5-epimerase 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A2Z7B33.0e-19278.84GDP-mannose 3,5-epimerase 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_032456... [more]
A3C4S45.1e-19278.84GDP-mannose 3,5-epimerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GME-1 PE... [more]
Q2R1V81.3e-19080.34GDP-mannose 3,5-epimerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GME-2 PE... [more]
Q93VR34.1e-18977.62GDP-mannose 3,5-epimerase OS=Arabidopsis thaliana OX=3702 GN=At5g28840 PE=1 SV=1[more]
Q6GMI91.0e-1924.00UDP-glucuronic acid decarboxylase 1 OS=Danio rerio OX=7955 GN=uxs1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KAT03.9e-20384.58GDP-mannose 3,5-epimerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111493789 PE=3 SV=... [more]
A0A6J1G3W33.9e-20384.58GDP-mannose 3,5-epimerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111450536 PE=3 S... [more]
A0A6J1CNH48.2e-20183.64GDP-mannose 3,5-epimerase 2 OS=Momordica charantia OX=3673 GN=LOC111013089 PE=3 ... [more]
A0A1S3C2Q94.1e-20083.64GDP-mannose 3,5-epimerase 2 OS=Cucumis melo OX=3656 GN=LOC103495988 PE=3 SV=1[more]
A0A5D3D4E24.1e-20083.64GDP-mannose 3,5-epimerase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
AT5G28840.12.9e-19077.62GDP-D-mannose 3',5'-epimerase [more]
AT5G28840.22.9e-19077.62GDP-D-mannose 3',5'-epimerase [more]
AT3G62830.17.0e-1924.13NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G62830.27.0e-1924.13NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47650.11.6e-1824.61UDP-xylose synthase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 30..248
e-value: 9.5E-42
score: 143.1
NoneNo IPR availableGENE3D3.90.25.10coord: 304..390
e-value: 3.2E-29
score: 103.3
NoneNo IPR availableGENE3D3.40.50.720coord: 27..271
e-value: 4.6E-49
score: 169.2
NoneNo IPR availablePANTHERPTHR43574EPIMERASE-RELATEDcoord: 301..417
NoneNo IPR availablePANTHERPTHR43574:SF55BNAC07G27420D PROTEINcoord: 301..417
NoneNo IPR availablePANTHERPTHR43574EPIMERASE-RELATEDcoord: 4..247
NoneNo IPR availablePANTHERPTHR43574:SF55BNAC07G27420D PROTEINcoord: 4..247
IPR033890GDP-mannose 3,5-epimeraseCDDcd05273GME-like_SDR_ecoord: 28..395
e-value: 0.0
score: 514.336
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 23..387

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003351.1Spg003351.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0047918 GDP-mannose 3,5-epimerase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0003824 catalytic activity