Spg003337 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003337 vs. NCBI nr
Match: XP_022946305.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita moschata] >XP_023547055.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita pepo subsp. pepo] >KAG7030363.1 Ubiquitin carboxyl-terminal hydrolase 22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 582/629 (92.53%), Postives = 594/629 (94.44%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF HHHHRHHPNLPFN+QIFPPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF-HHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR
Sbjct: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+ AP SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPWVPDLRERVL+GANSSPLDCANSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRYFCQRNVNVG KNS+ANDGNGSKNSR CLACDLD++FSAVFSGDR 
Sbjct: 241 PPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRI 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL G
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAATR+NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQ+AS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQNAS 607

BLAST of Spg003337 vs. NCBI nr
Match: XP_038889878.1 (ubiquitin C-terminal hydrolase 22-like [Benincasa hispida])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 583/629 (92.69%), Postives = 593/629 (94.28%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF HHHHRHHPNLPFN+QI PPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF-HHHHRHHPNLPFNSQISPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR
Sbjct: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+    SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPW+PDLRERVL+GANSSPLDCANSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWIPDLRERVLVGANSSPLDCANSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRYFCQRNVN G  NS+ANDGNGSKNSRICLACDLDAMFSAVFSGDRT
Sbjct: 241 PPLRNFFLSDRHNRYFCQRNVNGGNINSNANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           S+KMAA R+NLSCNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SSKMAAARSNLSCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 607

BLAST of Spg003337 vs. NCBI nr
Match: XP_022999050.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita maxima])

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 578/629 (91.89%), Postives = 589/629 (93.64%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF HHHHRHHPNLPFN+QIFP SCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF-HHHHRHHPNLPFNSQIFPTSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR
Sbjct: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+ AP SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPWVPDLRERVL+G NSSPLDCANSN GSES LPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWVPDLRERVLVGTNSSPLDCANSNPGSESALPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRYFCQRNVNVG KNS+ANDGNGSKNSR CLACDLD++FSAVFSGDR 
Sbjct: 241 PPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRI 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN  G
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNQAG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAATR NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAATR-NLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYK+SEKQ+AS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKSSEKQNAS 606

BLAST of Spg003337 vs. NCBI nr
Match: XP_008457196.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo] >KAA0063520.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa] >TYJ97847.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa])

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 572/629 (90.94%), Postives = 583/629 (92.69%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF      HHPNLPF +QIFPPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF------HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDR
Sbjct: 61  RRDPNEVPRCASCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+    SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPW+PDLRERVL+GANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRY+CQRNVN G  NS+ NDGNGSKNSRICLACDLDAMFSAVFSGDRT
Sbjct: 241 PPLRNFFLSDRHNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRT 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAA R+NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAAARSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of Spg003337 vs. NCBI nr
Match: XP_031736850.1 (ubiquitin C-terminal hydrolase 22 isoform X1 [Cucumis sativus])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 570/629 (90.62%), Postives = 581/629 (92.37%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF      HHPNLPF +QIFPPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF------HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDR
Sbjct: 61  RRDPNEVPRCSSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+    SQP LPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPSLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPW+PDLRERVL+GANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRYFCQRNVN    +S+ NDGNGSKNSRICLACDLDAMFSAVFSGDRT
Sbjct: 241 PPLRNFFLSDRHNRYFCQRNVNGSNVSSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRT 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                 K    +GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG
Sbjct: 361 -----VKRQNQSGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAA R+NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAAARSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDE D CNE SSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDELDGCNEMSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 609

BLAST of Spg003337 vs. ExPASy Swiss-Prot
Match: Q9LEW0 (Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 4.7e-141
Identity = 286/613 (46.66%), Postives = 367/613 (59.87%), Query Frame = 0

Query: 24  PPSCPHLADFR---SLNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPR 83
           P  C HL+D++     +G KSF  L  C        R  I+    ++PRC  C    + R
Sbjct: 11  PDPCNHLSDYKLRYGTDGYKSFNNLFRCFN----DARIKIKLQGIDIPRCSYC-SVYQKR 70

Query: 84  LYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAA 143
           LY C+ C ++SC      SH   H + +  GH+IA+DV+R+EL+CCAC DQVYD +FD  
Sbjct: 71  LYICLICRSISC-----SSHILLHTQ-LNKGHDIAIDVERSELYCCACIDQVYDSEFDEV 130

Query: 144 VVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVDYQPWVPDLRERVLMGAN--S 203
           VV  Q     +   + A   A  S          K+RR+D Q         +++G+N   
Sbjct: 131 VVSKQLFGLGMSVKSGADVVAVRS---------NKKRRLDSQ---------LIIGSNFLV 190

Query: 204 SPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQ 263
           SP D        +   P GLRGLNNLG+TCFMN+VLQAL+H PPLRNF+LS +HNR  C 
Sbjct: 191 SPRD-----RREKWTFPLGLRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCP 250

Query: 264 RNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANL 323
           R   +G+               +CL CDLD +FSA+FSGDRTPYSPA  L+SWWQH+ NL
Sbjct: 251 RR-TMGL---------------LCLPCDLDVIFSAMFSGDRTPYSPAHLLYSWWQHSTNL 310

Query: 324 ASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDC- 383
           A+YEQQD+HEFFIS+L+ IHE   K +                            N +C 
Sbjct: 311 ATYEQQDSHEFFISLLDRIHENEGKSK-----------------------CLYQDNEECQ 370

Query: 384 CIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEA 443
           CI H+ FSG+LRSDV C  CG TSTTYDP +DISL L+   G S   A  R N    G +
Sbjct: 371 CITHKAFSGLLRSDVTCTTCGSTSTTYDPFIDISLTLDSMNGFSP--ADCRKNRYSGGPS 430

Query: 444 DCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFH 503
                  N  + +L GCLD FTR EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H
Sbjct: 431 ------VNAIMPTLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLH 490

Query: 504 IKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGD---EQDACN 563
           +KRFEHS  RK SRK+D YLQ+PF L+M+PYLSSSI+  RFGNRIF FDG+   +  + +
Sbjct: 491 VKRFEHSLTRKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSS 542

Query: 564 ETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNENIVRAAQGYMMFYV 623
             S+EFE+FAV+THKG L++GHYVTYLRL   WY+CDDAWI +V E +VR  + YM+FY 
Sbjct: 551 SPSAEFEIFAVVTHKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYA 542

Query: 624 QKMLYYKASEKQS 628
           Q+ +  KA ++ S
Sbjct: 611 QETVIQKAHKELS 542

BLAST of Spg003337 vs. ExPASy Swiss-Prot
Match: Q5DU02 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Mus musculus OX=10090 GN=Usp22 PE=2 SV=2)

HSP 1 Score: 317.4 bits (812), Expect = 3.7e-85
Identity = 209/602 (34.72%), Postives = 294/602 (48.84%), Query Frame = 0

Query: 24  PPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRLYA 83
           PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG     RL++
Sbjct: 20  PPGCSHLGSFKVDNWKQNLRAIYQCF---VWSGTAEARKRKAKSCVCHVCGIHLN-RLHS 79

Query: 84  CVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAVVL 143
           C+ C    C    +  H   HA+S    H +A+D+    ++C  C+D +YD+D +     
Sbjct: 80  CLYCVFFGCF---TKKHIHDHAKS--KRHNLAIDLMYGGIYCFLCQDYIYDKDIEIIAKE 139

Query: 144 AQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVDYQPWVPDLRERVLMGANSSPLDC 203
            Q  A  + G                           +  W P  RE  L+  N      
Sbjct: 140 EQRKAWKMQGVG-----------------------EKFSTWEPTKRELELLKHNP----- 199

Query: 204 ANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNV 263
                   S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+     
Sbjct: 200 --KRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE----- 259

Query: 264 GIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQ 323
                        ++   CL C++ ++F   +SG R+P+ P K L   W HA +LA YEQ
Sbjct: 260 ------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQ 319

Query: 324 QDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDC-CIAHR 383
           QDAHEF I+ L+ +H     D                       G  +     C CI  +
Sbjct: 320 QDAHEFLIAALDVLHRHCKGD---------------------DNGKKANNPNHCNCIIDQ 379

Query: 384 VFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEADCMNS 443
           +F+G L+SDV C  C   STT DP  DISLDL    G S         LS   E   +N 
Sbjct: 380 IFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLP---GSSTPFWP----LSPGSEGSVVN- 439

Query: 444 GQNHR--ISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKR 503
           G++H    ++L  CL +FTRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+KR
Sbjct: 440 GESHASGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKR 499

Query: 504 FEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNETSSEF 563
           FEHS+  K+ RK+  Y+ FP  LDM P+++SS      G    P D    D      +++
Sbjct: 500 FEHSA--KLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPLDSLNND------NKY 525

Query: 564 ELFAVITHKGKLDAGHYVTYLRL-SDQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLY 622
            LFAV+ H+G L++GHY +++R   DQW+KCDDA IT+ +   V  ++GY++FY ++ L 
Sbjct: 560 SLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLE 525

BLAST of Spg003337 vs. ExPASy Swiss-Prot
Match: Q9UPT9 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Homo sapiens OX=9606 GN=USP22 PE=1 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 6.2e-85
Identity = 209/604 (34.60%), Postives = 295/604 (48.84%), Query Frame = 0

Query: 22  IFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRL 81
           + PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG     RL
Sbjct: 18  VAPPGCSHLGSFKVDNWKQNLRAIYQCF---VWSGTAEARKRKAKSCICHVCGVHLN-RL 77

Query: 82  YACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAV 141
           ++C+ C    C     H H  A A+     H +A+D+    ++C  C+D +YD+D +   
Sbjct: 78  HSCLYCVFFGCFT-KKHIHEHAKAKR----HNLAIDLMYGGIYCFLCQDYIYDKDMEIIA 137

Query: 142 VLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVDYQPWVPDLRERVLMGANSSPL 201
              Q  A  + G                           +  W P  RE  L+  N    
Sbjct: 138 KEEQRKAWKMQGVG-----------------------EKFSTWEPTKRELELLKHNP--- 197

Query: 202 DCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNV 261
                     S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+   
Sbjct: 198 ----KRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE--- 257

Query: 262 NVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASY 321
                          ++   CL C++ ++F   +SG R+P+ P K L   W HA +LA Y
Sbjct: 258 --------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGY 317

Query: 322 EQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDC-CIA 381
           EQQDAHEF I+ L+ +H     D                       G  +     C CI 
Sbjct: 318 EQQDAHEFLIAALDVLHRHCKGD---------------------DNGKKANNPNHCNCII 377

Query: 382 HRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEADCM 441
            ++F+G L+SDV C  C   STT DP  DISLDL    G S         LS   E + +
Sbjct: 378 DQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLP---GSSTPFWP----LSPGSEGNVV 437

Query: 442 NSGQNH--RISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHI 501
           N G++H    ++L  CL +FTRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+
Sbjct: 438 N-GESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHL 497

Query: 502 KRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNETSS 561
           KRFEHS+  K+ RK+  Y+ FP  LDM P+++SS      G    P D    D      +
Sbjct: 498 KRFEHSA--KLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNND------N 525

Query: 562 EFELFAVITHKGKLDAGHYVTYLRL-SDQWYKCDDAWITQVNENIVRAAQGYMMFYVQKM 621
           ++ LFAV+ H+G L++GHY +++R   DQW+KCDDA IT+ +   V  ++GY++FY ++ 
Sbjct: 558 KYSLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQF 525

BLAST of Spg003337 vs. ExPASy Swiss-Prot
Match: P0C8Z3 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Bos taurus OX=9913 GN=USP22 PE=2 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.8e-84
Identity = 208/604 (34.44%), Postives = 296/604 (49.01%), Query Frame = 0

Query: 22  IFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRL 81
           + PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG     RL
Sbjct: 7   VTPPGCAHLGSFKVDNWKQNLRAIYQCF---VWSGSAEARKRKAKSCVCHVCGLHLN-RL 66

Query: 82  YACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAV 141
           ++C+ C    C    +  H   HA+S    H +A+++    ++C  C+D +YD+D +   
Sbjct: 67  HSCLHCVFFGCF---TKKHIHEHAKS--KRHNLAIELMYGGIYCFLCQDYIYDKDIEIIA 126

Query: 142 VLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVDYQPWVPDLRERVLMGANSSPL 201
              Q  A  + G                           +  W P  RE  L+  N    
Sbjct: 127 KEEQRKAWKMQGVG-----------------------EKFSTWEPTKRELELLKHNP--- 186

Query: 202 DCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNV 261
                     S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+   
Sbjct: 187 ----KRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE--- 246

Query: 262 NVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASY 321
                          ++   CL C++ ++F   +SG R+P+ P K L   W HA +LA Y
Sbjct: 247 --------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGY 306

Query: 322 EQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDC-CIA 381
           EQQDAHEF I+ L+ +H     D                       G  +     C CI 
Sbjct: 307 EQQDAHEFLIAALDVLHRHCKGD---------------------DNGKKANNPNHCNCII 366

Query: 382 HRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEADCM 441
            ++F+G L+SDV C  C   STT DP  DISLDL    G S         LS   E+  +
Sbjct: 367 DQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLP---GSSTPFWP----LSPGSESSVV 426

Query: 442 NSGQNH--RISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHI 501
           N G++H    ++L  CL +FTRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+
Sbjct: 427 N-GESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHL 486

Query: 502 KRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNETSS 561
           KRFEHS+  K+ RK+  Y+ FP  LDM P+++SS      G    P D    D      +
Sbjct: 487 KRFEHSA--KLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNND------N 514

Query: 562 EFELFAVITHKGKLDAGHYVTYLRL-SDQWYKCDDAWITQVNENIVRAAQGYMMFYVQKM 621
           ++ LFAV+ H+G L++GHY +++R   DQW+KCDDA IT+ +   V  ++GY++FY ++ 
Sbjct: 547 KYSLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIADVLDSEGYLLFYHKQF 514

BLAST of Spg003337 vs. ExPASy Swiss-Prot
Match: A6H8I0 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.3e-84
Identity = 207/601 (34.44%), Postives = 291/601 (48.42%), Query Frame = 0

Query: 24  PPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPRLYA 83
           P  C H+  F+  N  ++ R++  C       G A  R+   +   C  CG     RL++
Sbjct: 3   PAGCSHVNGFKVDNWKQNLRVIYQCF---VWSGSAETRKRKAKSCICHMCGAHLN-RLHS 62

Query: 84  CVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAVVL 143
           C+ C    C    S  H   HA++    H +A+D+    ++C  C+D +YD+D +     
Sbjct: 63  CLHCVFFGCF---SKKHIHEHAKN--KRHNLAIDLLYGGIYCFVCQDYIYDKDMEQIAKE 122

Query: 144 AQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVDYQPWVPDLRERVLMGANSSPLDC 203
            Q  A  L G                           Y  W P  RE  L+  N      
Sbjct: 123 EQRKAWKLQGIG-----------------------EKYSMWEPTKRELELLRHNP----- 182

Query: 204 ANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNV 263
                   +    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+     
Sbjct: 183 --KRRKITANCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHK---CE----- 242

Query: 264 GIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQ 323
                          S  CL C++  +F   +SG R+P+ P + L   W HA +LA YEQ
Sbjct: 243 -------------MQSNSCLVCEMSQLFQEFYSGHRSPHIPFRLLHLVWTHARHLAGYEQ 302

Query: 324 QDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDCCIAHRV 383
           QDAHEF I+ L+ +H     D  K                       +  N   CI  ++
Sbjct: 303 QDAHEFLIAALDVLHRHCKDDNGKK---------------------ANNPNHCNCIIDQI 362

Query: 384 FSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEADCMNSG 443
           F+G L+SDV C  C   STT DP  DISLDL    G S         LS   +   +N G
Sbjct: 363 FTGGLQSDVTCQVCHGVSTTIDPFWDISLDLP---GSSTPFWP----LSPGSDGSVVN-G 422

Query: 444 QNH--RISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRF 503
            +H    ++L  CL +FTRPE LGS  K  C  C   QES KQ+++++LP+V+CFH+KRF
Sbjct: 423 DSHPSGATTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKRLPIVACFHLKRF 482

Query: 504 EHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNETSSEFE 563
           EHS+  K+ RK+  Y+ FP  LDM P+++SS      G    P D    D      +++ 
Sbjct: 483 EHSA--KLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPVDSLNND------NKYS 506

Query: 564 LFAVITHKGKLDAGHYVTYLRL-SDQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYY 622
           LFAV+ H+G L++GHY T++R   DQW+KCDDA IT+ +   V  ++GY++FY ++ L Y
Sbjct: 543 LFAVVNHQGTLESGHYTTFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEY 506

BLAST of Spg003337 vs. ExPASy TrEMBL
Match: A0A6J1G3H3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450426 PE=3 SV=1)

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 582/629 (92.53%), Postives = 594/629 (94.44%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF HHHHRHHPNLPFN+QIFPPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF-HHHHRHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR
Sbjct: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+ AP SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPWVPDLRERVL+GANSSPLDCANSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRYFCQRNVNVG KNS+ANDGNGSKNSR CLACDLD++FSAVFSGDR 
Sbjct: 241 PPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRI 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL G
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAATR+NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAATRSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQ+AS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQNAS 607

BLAST of Spg003337 vs. ExPASy TrEMBL
Match: A0A6J1KE79 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493554 PE=3 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 578/629 (91.89%), Postives = 589/629 (93.64%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF HHHHRHHPNLPFN+QIFP SCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF-HHHHRHHPNLPFNSQIFPTSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR
Sbjct: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+ AP SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPWVPDLRERVL+G NSSPLDCANSN GSES LPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWVPDLRERVLVGTNSSPLDCANSNPGSESALPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRYFCQRNVNVG KNS+ANDGNGSKNSR CLACDLD++FSAVFSGDR 
Sbjct: 241 PPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAVFSGDRI 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN  G
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNQAG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAATR NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAATR-NLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYK+SEKQ+AS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKSSEKQNAS 606

BLAST of Spg003337 vs. ExPASy TrEMBL
Match: A0A5D3BFE3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00590 PE=3 SV=1)

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 572/629 (90.94%), Postives = 583/629 (92.69%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF      HHPNLPF +QIFPPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF------HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDR
Sbjct: 61  RRDPNEVPRCASCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+    SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPW+PDLRERVL+GANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRY+CQRNVN G  NS+ NDGNGSKNSRICLACDLDAMFSAVFSGDRT
Sbjct: 241 PPLRNFFLSDRHNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRT 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAA R+NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAAARSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of Spg003337 vs. ExPASy TrEMBL
Match: A0A1S3C4I9 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo OX=3656 GN=LOC103496929 PE=3 SV=1)

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 572/629 (90.94%), Postives = 583/629 (92.69%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF      HHPNLPF +QIFPPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF------HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDR
Sbjct: 61  RRDPNEVPRCASCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+    SQPLLPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPW+PDLRERVL+GANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRY+CQRNVN G  NS+ NDGNGSKNSRICLACDLDAMFSAVFSGDRT
Sbjct: 241 PPLRNFFLSDRHNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRT 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAA R+NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAAARSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDEQD CNETSSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDEQDGCNETSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of Spg003337 vs. ExPASy TrEMBL
Match: A0A0A0LL31 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G008080 PE=3 SV=1)

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 569/629 (90.46%), Postives = 579/629 (92.05%), Query Frame = 0

Query: 1   MSSNFHHHHHRHHPNLPFNTQIFPPSCPHLADFRSLNGSKSFRLLQDCLRVKPPGGRAAI 60
           MSSNF      HHPNLPF +QIFPPSCPHLADFRS NGSKSFRLLQDCLRVKPPGGRAAI
Sbjct: 1   MSSNF------HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAI 60

Query: 61  RRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDR 120
           RRDPNEVPRC SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDR
Sbjct: 61  RRDPNEVPRCSSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDR 120

Query: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVD 180
           AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAV+S+    SQP LPPENLRKRRRVD
Sbjct: 121 AELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPSLPPENLRKRRRVD 180

Query: 181 YQPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240
           YQPW+PDLRERVL+GANSSPLDC NSN GSESELPWGLRGLNNLGNTCFMNSVLQALLHT
Sbjct: 181 YQPWMPDLRERVLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHT 240

Query: 241 PPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRT 300
           PPLRNFFLSDRHNRYFCQRNVN    +S+ NDGNGSKNSRICLACDLDAMFSAVFSGDRT
Sbjct: 241 PPLRNFFLSDRHNRYFCQRNVNGSNVSSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRT 300

Query: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTI 360
           PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ         
Sbjct: 301 PYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ--------- 360

Query: 361 INLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420
                       GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG
Sbjct: 361 ------------GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGG 420

Query: 421 SAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480
           SAKMAA R+NL CNGEADCMNS QN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE
Sbjct: 421 SAKMAAARSNLPCNGEADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQE 480

Query: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540
           SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN
Sbjct: 481 SLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGN 540

Query: 541 RIFPFDGDEQDACNETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNE 600
           RIFPFDGDE D CNE SSEFELFAVITHKGKLDAGHYVTYLRLS+QWYKCDDAWITQVNE
Sbjct: 541 RIFPFDGDELDGCNEMSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNE 600

Query: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 630
           NIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 601 NIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of Spg003337 vs. TAIR 10
Match: AT5G10790.1 (ubiquitin-specific protease 22 )

HSP 1 Score: 503.1 bits (1294), Expect = 3.3e-142
Identity = 286/613 (46.66%), Postives = 367/613 (59.87%), Query Frame = 0

Query: 24  PPSCPHLADFR---SLNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCGSCGDSARPR 83
           P  C HL+D++     +G KSF  L  C        R  I+    ++PRC  C    + R
Sbjct: 11  PDPCNHLSDYKLRYGTDGYKSFNNLFRCFN----DARIKIKLQGIDIPRCSYC-SVYQKR 70

Query: 84  LYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAA 143
           LY C+ C ++SC      SH   H + +  GH+IA+DV+R+EL+CCAC DQVYD +FD  
Sbjct: 71  LYICLICRSISC-----SSHILLHTQ-LNKGHDIAIDVERSELYCCACIDQVYDSEFDEV 130

Query: 144 VVLAQTTASTLGGNAVASTAAPASQPLLPPENLRKRRRVDYQPWVPDLRERVLMGAN--S 203
           VV  Q     +   + A   A  S          K+RR+D Q         +++G+N   
Sbjct: 131 VVSKQLFGLGMSVKSGADVVAVRS---------NKKRRLDSQ---------LIIGSNFLV 190

Query: 204 SPLDCANSNSGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQ 263
           SP D        +   P GLRGLNNLG+TCFMN+VLQAL+H PPLRNF+LS +HNR  C 
Sbjct: 191 SPRD-----RREKWTFPLGLRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCP 250

Query: 264 RNVNVGIKNSSANDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANL 323
           R   +G+               +CL CDLD +FSA+FSGDRTPYSPA  L+SWWQH+ NL
Sbjct: 251 RR-TMGL---------------LCLPCDLDVIFSAMFSGDRTPYSPAHLLYSWWQHSTNL 310

Query: 324 ASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDC- 383
           A+YEQQD+HEFFIS+L+ IHE   K +                            N +C 
Sbjct: 311 ATYEQQDSHEFFISLLDRIHENEGKSK-----------------------CLYQDNEECQ 370

Query: 384 CIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEA 443
           CI H+ FSG+LRSDV C  CG TSTTYDP +DISL L+   G S   A  R N    G +
Sbjct: 371 CITHKAFSGLLRSDVTCTTCGSTSTTYDPFIDISLTLDSMNGFSP--ADCRKNRYSGGPS 430

Query: 444 DCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFH 503
                  N  + +L GCLD FTR EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H
Sbjct: 431 ------VNAIMPTLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLH 490

Query: 504 IKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGD---EQDACN 563
           +KRFEHS  RK SRK+D YLQ+PF L+M+PYLSSSI+  RFGNRIF FDG+   +  + +
Sbjct: 491 VKRFEHSLTRKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSS 542

Query: 564 ETSSEFELFAVITHKGKLDAGHYVTYLRLSDQWYKCDDAWITQVNENIVRAAQGYMMFYV 623
             S+EFE+FAV+THKG L++GHYVTYLRL   WY+CDDAWI +V E +VR  + YM+FY 
Sbjct: 551 SPSAEFEIFAVVTHKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYA 542

Query: 624 QKMLYYKASEKQS 628
           Q+ +  KA ++ S
Sbjct: 611 QETVIQKAHKELS 542

BLAST of Spg003337 vs. TAIR 10
Match: AT5G57990.1 (ubiquitin-specific protease 23 )

HSP 1 Score: 139.0 bits (349), Expect = 1.3e-32
Identity = 105/399 (26.32%), Postives = 164/399 (41.10%), Query Frame = 0

Query: 220 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNS 279
           GL NLGNTCF+NSVLQ L +T PL     +  H +Y                       +
Sbjct: 108 GLQNLGNTCFLNSVLQCLTYTEPLAATLQTAAHQKY--------------------CHVA 167

Query: 280 RICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHE 339
             C  C +          +    +P   + +    + N  +  Q+DAHE+ I++LE +H+
Sbjct: 168 GFCALCAIQKHVRTARQANGRILAPKDLVSNLRCISRNFRNCRQEDAHEYMINLLECMHK 227

Query: 340 KVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGF 399
                     S  A   +L                      H++F G LRS V C  C  
Sbjct: 228 CSLPSGVPSESSDAYRRSL---------------------VHKIFGGSLRSQVKCEQCSH 287

Query: 400 TSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFT 459
            S  +DP +D+SLD+                                +  SL   L +FT
Sbjct: 288 CSNKFDPFLDLSLDIS-------------------------------KADSLQRALSRFT 347

Query: 460 RPEKLGSDQKFF-CQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQ 519
             E L +  K + C++C+ + ++ KQ+++ K P V   H+KRFE     K+ RKVD    
Sbjct: 348 AVELLDNGAKVYQCERCKQKVKAKKQLTVSKAPYVLTVHLKRFEAHRSEKIDRKVD---- 407

Query: 520 FPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNETSSEFELFAVITHKGKLD-AGHY 579
           F  ++DM P++S                       +E + ++ L+ V+ H G+   +GHY
Sbjct: 408 FTSAIDMKPFVSGP---------------------HEGNLKYTLYGVLVHYGRSSHSGHY 409

Query: 580 VTYLRLSD-QWYKCDDAWITQVNENIVRAAQGYMMFYVQ 616
             ++R S   WY  DD  + QV+E  V   + YM+FYV+
Sbjct: 468 ACFVRTSSGMWYSLDDNRVVQVSEKTVFNQKAYMLFYVR 409

BLAST of Spg003337 vs. TAIR 10
Match: AT4G17895.1 (ubiquitin-specific protease 20 )

HSP 1 Score: 137.9 bits (346), Expect = 2.8e-32
Identity = 116/448 (25.89%), Postives = 193/448 (43.08%), Query Frame = 0

Query: 182 QPWVPDLRERVLMGANSSPLDCANSNSGSESELPWGLR-----------GLNNLGNTCFM 241
           +PW P++        +    D  NS +     L +G R           GL NLGN+CF+
Sbjct: 129 RPWTPNVSPG-FGSDDDDDNDDDNSKNEPRKSLFYGFRQEPEPVTGVGAGLWNLGNSCFL 188

Query: 242 NSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNSRICLACDLDAM 301
           NSV Q   HT PL    LS R+     +   + G             N   C+   +   
Sbjct: 189 NSVFQCFTHTVPLIESLLSFRY-----EVPCHCG-------------NEFFCVIRAIRYH 248

Query: 302 FSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYS 361
             A    +R P +P  F  +    + +   Y+Q+DAHEF  + LE + E    DR     
Sbjct: 249 IEAALRPERCPIAPYFFFDNLNYFSPDFQRYQQEDAHEFLQAFLEKL-EICGSDRTS--- 308

Query: 362 QGALLSTLTIINLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDI 421
                          R  +TS           VFSG L S + C  C + S TY+  V +
Sbjct: 309 --------------FRGDITS---------QDVFSGRLISGLRCCNCDYVSETYEKSVGL 368

Query: 422 SLDLEPNLGGSAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFTRPEKLGSDQKF 481
           SL++E                                + +L   L+ FTR EKL  D++ 
Sbjct: 369 SLEIED-------------------------------VDTLGSALESFTRVEKL--DEQL 428

Query: 482 FCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLS 541
            C  C  +    KQ+ + KLPLV+ FH+KRF+++ +     K+ ++++ P  +D+ PY+ 
Sbjct: 429 TCDNCNEKVSKEKQLLLDKLPLVATFHLKRFKNNGL--YMEKIYKHVKIPLEIDLQPYMR 476

Query: 542 SSILRSRFGNRIFPFDGDEQDACNETSSEFELFAVITHKG-KLDAGHYVTYLRLSDQ-WY 601
           +                  Q+  NE S+++ L+A++ H G  +  GHY +Y+R + + W+
Sbjct: 489 NI-----------------QE--NEVSTKYHLYALVEHFGYSVAYGHYSSYVRSAPKIWH 476

Query: 602 KCDDAWITQVNENIVRAAQGYMMFYVQK 617
             DD+ +T+++E++V +   Y++FY ++
Sbjct: 549 HFDDSKVTRIDEDMVLSQDSYILFYARE 476

BLAST of Spg003337 vs. TAIR 10
Match: AT3G14400.1 (ubiquitin-specific protease 25 )

HSP 1 Score: 124.8 bits (312), Expect = 2.5e-28
Identity = 100/396 (25.25%), Postives = 166/396 (41.92%), Query Frame = 0

Query: 220 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNS 279
           GL NLGNTC++NSVLQ L  TPPL NF L+ +H+ + C   V+              +  
Sbjct: 25  GLRNLGNTCYLNSVLQCLTFTPPLANFCLTHKHSSH-CDTYVD-------------GERK 84

Query: 280 RICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHE 339
           R C  C ++   +   S D T  +P K        A +     Q+DAHEF   +++  H 
Sbjct: 85  RDCPFCIVEKRIARSLSVDLTTDAPNKISSCLKIFAEHFKLGRQEDAHEFLRYVIDACHN 144

Query: 340 KVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMACGF 399
                           ++L +  L         GN    +   +F G L+S V C++CG 
Sbjct: 145 ----------------TSLRLKKLRYNGNEPFNGNS---VVKEIFGGALQSQVKCLSCGA 204

Query: 400 TSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLDQFT 459
            S   D  +DISL++  +                               SS+   L +F 
Sbjct: 205 ESNKADEIMDISLEILQS-------------------------------SSVKESLQKFF 264

Query: 460 RPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQF 519
           + E L  + K+ C+ C+    + KQMSI + P +    +KRF          K+D+ + F
Sbjct: 265 QSEILDGNNKYRCESCEKLVTARKQMSILQAPNILVIQLKRFGGI----FGGKIDKAISF 324

Query: 520 PFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNETSSEFELFAVITHKG-KLDAGHYV 579
              L ++ ++S                     A  +   E++LF +I H G   ++GHY 
Sbjct: 325 GEILVLSNFMSK--------------------ASKDPQPEYKLFGIIVHSGFSPESGHYY 332

Query: 580 TYLRLS-DQWYKCDDAWITQVNENIVRAAQGYMMFY 614
            Y++ S  +WY C+D++++      V + + Y++F+
Sbjct: 385 AYVKDSLGRWYCCNDSFVSLSTLQEVLSEKAYILFF 332

BLAST of Spg003337 vs. TAIR 10
Match: AT2G24640.1 (ubiquitin-specific protease 19 )

HSP 1 Score: 119.0 bits (297), Expect = 1.4e-26
Identity = 100/401 (24.94%), Postives = 162/401 (40.40%), Query Frame = 0

Query: 220 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGIKNSSANDGNGSKNS 279
           GL N GN+CF N VLQ L  T PL  + L   H R  C+RN                   
Sbjct: 175 GLTNCGNSCFANVVLQCLSWTRPLVAYLLERGHKRE-CRRN------------------- 234

Query: 280 RICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHE---FFISILEG 339
             C  C+ +       +  R P+SP   +        NL    Q+DAHE   F I +++ 
Sbjct: 235 DWCFLCEFENHLDRA-NYSRFPFSPMNIISRLPNIGGNLGYGRQEDAHELMRFAIDMMQS 294

Query: 340 IHEKVDKDRRKPYSQGALLSTLTIINLFMRKGLTSAGNGDCCIAHRVFSGILRSDVMCMA 399
           +                       ++ F  + +      +  +   +F G+L+S V C A
Sbjct: 295 V----------------------CLDEFGGEKVVPPRAQETTLIQYIFGGLLQSQVQCTA 354

Query: 400 CGFTSTTYDPCVDISLDLEPNLGGSAKMAATRANLSCNGEADCMNSGQNHRISSLVGCLD 459
           C   S  Y+  +D+++++                   +G+A            SL  CLD
Sbjct: 355 CSNVSDQYENMMDLTVEI-------------------HGDA-----------VSLEECLD 414

Query: 460 QFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRY 519
           QFT  E L  D  + C +C    ++ K++SIR  P +    +KRF+     K+++++   
Sbjct: 415 QFTAKEWLQGDNLYKCDRCDDYVKACKRLSIRCAPNILTIALKRFQGGRFGKLNKRI--- 474

Query: 520 LQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNETSSEFELFAVITHKGKLDA-- 579
             FP + D+ PY+S                        E S  ++L+AVI H   L+A  
Sbjct: 475 -SFPETFDLGPYMSGG---------------------GEGSDVYKLYAVIVHLDMLNASF 477

Query: 580 -GHYVTYLR-LSDQWYKCDDAWITQVNENIVRAAQGYMMFY 614
            GHY+ Y++     WY+ DD+ + +V    V + + YM+ Y
Sbjct: 535 FGHYICYVKDFRGNWYRIDDSEVEKVELEDVLSQRAYMLLY 477