Spg003161 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003161 vs. NCBI nr
Match: XP_038891276.1 (GDSL esterase/lipase At1g29670-like [Benincasa hispida])

HSP 1 Score: 385.2 bits (988), Expect = 5.3e-103
Identity = 201/371 (54.18%), Postives = 230/371 (61.99%), Query Frame = 0

Query: 27  MKAQLMV--YCFLTIAA---FGTRGVDG-----KSILPCYFVFGDSQADNGNNNIL---N 86
           MK +L++  YC + I        + VDG     K  + CYFVFGDSQADNGNNN +    
Sbjct: 1   MKGELLMVYYCLIIIIGGLLLENKYVDGASSSSKKKVSCYFVFGDSQADNGNNNDMLERE 60

Query: 87  IGMARADYKPYGIDFSKQQTPTGRFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGV 146
            G ARADYKPYGIDFSKQ  PTGRFTNGRN+PDFIAKFLGF +YIPPF+ TK   ILKGV
Sbjct: 61  YGNARADYKPYGIDFSKQSIPTGRFTNGRNVPDFIAKFLGFRDYIPPFKITKSRTILKGV 120

Query: 147 NYASGGSGILPETGRLLS------------------------------------------ 206
           NYASGG+GIL ETGR L                                           
Sbjct: 121 NYASGGAGILRETGRTLGQVSSIKKQLENHNYTISQMHGLLGSKTKITSYLKRCLYTIQI 180

Query: 207 ----------------------------------------LYEQGARKVAVFGVGSIGCT 266
                                                   LY+QGARKVA+FGVGSIGCT
Sbjct: 181 GSNDYLNNFFMPKLYQTSSQYSPKAYAIALNKELSTQLKILYDQGARKVAIFGVGSIGCT 240

Query: 267 PYARANFEHKGAPCVDKINSAVQLFNIGLKSLVEDLNTQFPDAKFIFVDVFQISTIQPSN 303
           PYARANFEH+G PCVDK+NSA+QLFN GLKSLV+ LNT  P AKF F+DVF+ISTI PS+
Sbjct: 241 PYARANFEHRGLPCVDKMNSAIQLFNDGLKSLVQQLNTNLPAAKFTFIDVFKISTIDPSD 300

BLAST of Spg003161 vs. NCBI nr
Match: KAE8653340.1 (hypothetical protein Csa_007668 [Cucumis sativus])

HSP 1 Score: 370.9 bits (951), Expect = 1.0e-98
Identity = 177/276 (64.13%), Postives = 211/276 (76.45%), Query Frame = 0

Query: 31  LMVYCFLTIAAFGTRGVDG----KSILPCYFVFGDSQADNGNNNILNIGMARADYKPYGI 90
           L +Y FLT+  F +  V+G     S + CYF+FGDS  DNGNNN  N G+ARADYKPYGI
Sbjct: 12  LNIYLFLTMFGFESYWVNGASSSNSKVGCYFIFGDSLVDNGNNN-NNKGLARADYKPYGI 71

Query: 91  DFSKQQTPTGRFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPET 150
           DFSK   PTGRFTNGRNI DFIA+FL F NYIPPF+NT+  +ILKGVNYASG +GI  ET
Sbjct: 72  DFSKNMIPTGRFTNGRNIADFIAEFLSFKNYIPPFKNTRGWNILKGVNYASGAAGIRDET 131

Query: 151 GRLLSLYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVED 210
           G    LYE+GARKVA+FG G +GC+PYA+A F+HKG+ CVDKIN+A+QLFNIGLKSLV+D
Sbjct: 132 GMTQGLYEKGARKVAIFGGGIVGCSPYAKAKFDHKGSSCVDKINNAIQLFNIGLKSLVKD 191

Query: 211 LNTQFPDAKFIFVDVFQISTIQ-PSNFGKMVLEEPCCEVLPGQLQCSPFGKVCGDRSQYL 270
            NT F DA FIF+DVF I+     SN G +  + PCCE+    LQC   GKVCG+RS+Y+
Sbjct: 192 FNTNFGDANFIFIDVFNIALHDTSSNQGVINRDNPCCELRGDGLQCEVNGKVCGNRSEYI 251

Query: 271 FWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHL 302
           FWDGVHPTEIG  T+A+RAFN+Q P DT+PFDI HL
Sbjct: 252 FWDGVHPTEIGMMTLATRAFNAQHPNDTYPFDINHL 286

BLAST of Spg003161 vs. NCBI nr
Match: XP_011649033.1 (GDSL esterase/lipase At1g29670 [Cucumis sativus] >XP_031737227.1 GDSL esterase/lipase At1g29670 [Cucumis sativus] >KGN61342.1 hypothetical protein Csa_005903 [Cucumis sativus])

HSP 1 Score: 368.6 bits (945), Expect = 5.1e-98
Identity = 186/346 (53.76%), Postives = 210/346 (60.69%), Query Frame = 0

Query: 45  RGVDGKSILPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDFSKQQT---PTGRF 104
           +G  G+ I  CYFVFGDSQADNGNNN +     G ARADYKPYGIDFS   +   PTGRF
Sbjct: 37  KGGSGRGICSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFSSSSSSYIPTGRF 96

Query: 105 TNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLL-------- 164
           TN RN+PDFIAKFLGF +YIPPF  TK   ILKG NYASGG+GIL ETGR L        
Sbjct: 97  TNARNVPDFIAKFLGFDDYIPPFRTTKSRTILKGANYASGGAGILRETGRTLGQVSSIKK 156

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 157 QLENHNYTISQMHTLLGSSSKINRYLKECLYTVQIGSNDYLNNYFMPKIYMTSTQYSPQA 216

Query: 225 --------------SLYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLF 284
                         +LY  GARKVA+FGVGSIGCTPYAR NFEH G PCVD+INSA+QLF
Sbjct: 217 FATALNKNLSQHLKNLYNYGARKVAIFGVGSIGCTPYARENFEHTGLPCVDEINSAIQLF 276

Query: 285 NIGLKSLVEDLNTQFPDAKFIFVDVFQISTIQPSNFGKMVLEEPCCEVLPGQLQCSPFGK 303
           N GLKSLV+ LN   P AKF F+DVFQIST+ P N+GKMVL+ PCCEV  G +QCSPFGK
Sbjct: 277 NSGLKSLVQHLNANLPSAKFTFIDVFQISTVDPLNYGKMVLDAPCCEVGAGAMQCSPFGK 336

BLAST of Spg003161 vs. NCBI nr
Match: XP_022144504.1 (GDSL esterase/lipase At1g29670-like [Momordica charantia])

HSP 1 Score: 356.7 bits (914), Expect = 2.0e-94
Identity = 180/340 (52.94%), Postives = 216/340 (63.53%), Query Frame = 0

Query: 50  KSILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQ--TPTGRFTNGRNIPDF 109
           KS + CYF+FGDSQ+DNGNNN +  G A A+YKPYGIDF  Q   TPTGRFTNGRN+PDF
Sbjct: 55  KSRMDCYFIFGDSQSDNGNNNDIK-GDAMANYKPYGIDFPNQNSPTPTGRFTNGRNLPDF 114

Query: 110 IAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLL----------------- 169
           IA+ LGF +YIPPF NT   ++LKGVNYASGGSGI  ETGR+L                 
Sbjct: 115 IAELLGFHDYIPPFRNTTGWNVLKGVNYASGGSGIHSETGRMLGKVLSINKQLENHNHTI 174

Query: 170 ------------------------------------------------------------ 229
                                                                       
Sbjct: 175 SRIQGSLGSKLAATKHLKSCLYTVQAGSNDYLNNYFLPFSYKTSAQFTPQQYAVALNKQL 234

Query: 230 -----SLYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVE 289
                 LY++G RKVAVFGVG IGCTPYARANFEH+G+PCV+KIN A+QLFN GLKSL+ 
Sbjct: 235 SHQLEELYDRGGRKVAVFGVGGIGCTPYARANFEHEGSPCVEKINYAIQLFNSGLKSLIH 294

Query: 290 DLNTQFPDAKFIFVDVFQISTIQPSNF---GKMVLEEPCCEVLPGQLQCSPFGKVCGDRS 303
           DLNT+F DAKFIF+DVF+IST++P+       +VL+ PCCEV  GQLQC+PFGKVCG+RS
Sbjct: 295 DLNTKFTDAKFIFIDVFKISTMKPTPLVPADLLVLDAPCCEVPVGQLQCTPFGKVCGNRS 354

BLAST of Spg003161 vs. NCBI nr
Match: XP_008441762.1 (PREDICTED: GDSL esterase/lipase At1g29670-like [Cucumis melo] >KAA0051383.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK23839.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 355.1 bits (910), Expect = 5.9e-94
Identity = 182/348 (52.30%), Postives = 208/348 (59.77%), Query Frame = 0

Query: 45  RGVDGKSILPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDF-----SKQQTPTG 104
           +G  G     CYFVFGDSQADNGNNN +     G ARADYKPYGIDF     S    PTG
Sbjct: 36  KGGGGSDACSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTG 95

Query: 105 RFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS----- 164
           RFTNGRN+PDFIA+FLGF +YIPPF  TK   ILKG NYASGG+GIL ETGR L      
Sbjct: 96  RFTNGRNVPDFIAQFLGFRDYIPPFRTTKSQTILKGANYASGGAGILLETGRTLGEVSSI 155

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 156 KKQLENHNYTISQMQRLFGSSSKLKSYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSP 215

Query: 225 -----------------LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQ 284
                            LYEQGARKVA+FGVGSIGCTPYARANFEH G PCVDKIN+A+Q
Sbjct: 216 QAFATALNEDLSRQLKILYEQGARKVAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQ 275

Query: 285 LFNIGLKSLVEDLNTQFPDAKFIFVDVFQISTIQPSNFGKMVLEEPCCEVLPGQLQCSPF 303
           LFN GLKSLVE LN   P AKF F+DVF+IST+ P N+GK+ L+ PCCEV  G++QC+PF
Sbjct: 276 LFNAGLKSLVEHLNANLPSAKFTFIDVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPF 335

BLAST of Spg003161 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.3e-55
Identity = 132/365 (36.16%), Postives = 173/365 (47.40%), Query Frame = 0

Query: 27  MKAQLMVYC-FLTIAAFGTRGV--DGKSILPCYFVFGDSQADNGNNNILNIGMARADYKP 86
           M++ L  +C  L +  FG   V    ++ +PC+FVFGDS  DNGNNN L I +AR++Y P
Sbjct: 1   MESYLTKWCVVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGL-ISIARSNYFP 60

Query: 87  YGIDFSKQQTPTGRFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGIL 146
           YGIDF     PTGRF+NG+   D IA+ LGF  YIP +       IL GVNYAS  +GI 
Sbjct: 61  YGIDFG---GPTGRFSNGKTTVDVIAELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIR 120

Query: 147 PETGRLL----------------------------------------------------- 206
            ETGR L                                                     
Sbjct: 121 EETGRQLGQRISFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYF 180

Query: 207 -----------------------------SLYEQGARKVAVFGVGSIGCTPYARANFEHK 266
                                        +LY  GARK A+ G+G++GC+P A A     
Sbjct: 181 MPTFYSSSRQFTPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALAG-SPD 240

Query: 267 GAPCVDKINSAVQLFNIGLKSLVEDLNTQFPDAKFIFVD---VFQISTIQPSNFGKMVLE 302
           G  CVD+INSA Q+FN  L+SLV+ LN   PDAKFI+++   +FQ     P+ FG  V  
Sbjct: 241 GRTCVDRINSANQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTN 300

BLAST of Spg003161 vs. ExPASy Swiss-Prot
Match: Q93YW8 (GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.4e-52
Identity = 119/340 (35.00%), Postives = 155/340 (45.59%), Query Frame = 0

Query: 49  GKSILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNIPDFI 108
           G  I PCYF+FGDS  D+GNNN L   +ARA+Y PYGIDF  Q  PTGRF+NG+   D I
Sbjct: 22  GDPIAPCYFIFGDSLVDSGNNNRLT-SLARANYFPYGIDF--QYGPTGRFSNGKTTVDVI 81

Query: 109 AKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS----------------- 168
            + LGF +YI P+   +  DIL+GVNYAS  +GI  ETGR L                  
Sbjct: 82  TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVS 141

Query: 169 ------------------------------------------------------------ 228
                                                                       
Sbjct: 142 QVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYT 201

Query: 229 -----LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVED 288
                +Y  GARK A+ G+G+IGC+P   A     G  C ++INSA ++FN  L SLV+ 
Sbjct: 202 EQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDH 261

Query: 289 LNTQFPDAKFIFVD---VFQISTIQPSNFGKMVLEEPCCEV--LPGQLQCSPFGKVCGDR 302
            N   P AKF +++   +FQ     PS +G  V    CC V    GQ+ C P    C +R
Sbjct: 262 FNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNR 321

BLAST of Spg003161 vs. ExPASy Swiss-Prot
Match: Q9C7N5 (GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.9e-51
Identity = 123/366 (33.61%), Postives = 168/366 (45.90%), Query Frame = 0

Query: 27  MKAQLMVYCFLTI----AAFGTRGVDGKSILPCYFVFGDSQADNGNNNILNIGMARADYK 86
           M++ L  +C +++       G + V  +  +PCYF+FGDS  DNGNNN L   +ARADY 
Sbjct: 1   MESYLRKWCLVSVWVLLLGLGFK-VKAEPQVPCYFIFGDSLVDNGNNNRLR-SIARADYF 60

Query: 87  PYGIDFSKQQTPTGRFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGI 146
           PYGIDF     PTGRF+NGR   D + + LGF NYIP +      +IL+GVNYAS  +GI
Sbjct: 61  PYGIDFG---GPTGRFSNGRTTVDVLTELLGFDNYIPAYSTVSGQEILQGVNYASAAAGI 120

Query: 147 LPETG------------------------------------------------------- 206
             ETG                                                       
Sbjct: 121 REETGAQLGQRITFSGQVENYKNTVAQVVEILGDEYTAADYLKRCIYSVGMGSNDYLNNY 180

Query: 207 ---------------------------RLLSLYEQGARKVAVFGVGSIGCTPYARANFEH 266
                                      +L +LY  GARK A+ G+G+IGC+P A A    
Sbjct: 181 FMPQFYSTSRQYTPEQYADDLISRYRDQLNALYNYGARKFALVGIGAIGCSPNALAQGSQ 240

Query: 267 KGAPCVDKINSAVQLFNIGLKSLVEDLNTQFPDAKFIFVD---VFQISTIQPSNFGKMVL 302
            G  CV++INSA ++FN  L S+V+ LN    DA F +++    FQ     PS +G    
Sbjct: 241 DGTTCVERINSANRIFNNRLISMVQQLNNAHSDASFTYINAYGAFQDIIANPSAYGFTNT 300

BLAST of Spg003161 vs. ExPASy Swiss-Prot
Match: Q9FK75 (GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.9e-51
Identity = 119/337 (35.31%), Postives = 156/337 (46.29%), Query Frame = 0

Query: 52  ILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNIPDFIAKF 111
           I PCYF+FGDS  DNGNNN L   +ARA+Y PYGIDF+    PTGRF+NG    D IA+ 
Sbjct: 26  IAPCYFIFGDSLVDNGNNNQLQ-SLARANYFPYGIDFA--AGPTGRFSNGLTTVDVIAQL 85

Query: 112 LGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS-------------------- 171
           LGF +YI P+ + +  DIL+GVNYAS  +GI  ETGR L                     
Sbjct: 86  LGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVV 145

Query: 172 ------------------------------------------------------------ 231
                                                                       
Sbjct: 146 NILGDQNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQL 205

Query: 232 --LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVEDLNT 291
             LY  GARK A+ GVG+IGC+P   A     G  C ++INSA ++FN  L S+V+  N 
Sbjct: 206 RVLYTNGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQ 265

Query: 292 QFPDAKFIFVD---VFQISTIQPSNFGKMVLEEPCCEV--LPGQLQCSPFGKVCGDRSQY 302
             PDAKF +++   +FQ     P+ +G  V    CC V    GQ+ C P    C +R++Y
Sbjct: 266 NTPDAKFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEY 325

BLAST of Spg003161 vs. ExPASy Swiss-Prot
Match: Q9SZW7 (GDSL esterase/lipase At4g30140 OS=Arabidopsis thaliana OX=3702 GN=At4g30140 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.4e-50
Identity = 122/324 (37.65%), Postives = 152/324 (46.91%), Query Frame = 0

Query: 54  PCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNIPDFIAKFLG 113
           PCYFVFGDS  DNGNNN LN   A+ +Y PYGID+   Q PTGRF+NGRNIPD IA+  G
Sbjct: 32  PCYFVFGDSVFDNGNNNALNT-KAKVNYLPYGIDYF--QGPTGRFSNGRNIPDVIAELAG 91

Query: 114 FVNYIPPFENTKDSDILKGVNYASGGSGILPETGR------------------------- 173
           F N IPPF     +    G+NYASG  GI  ET                           
Sbjct: 92  FNNPIPPFAGASQAQANIGLNYASGAGGIREETSENMGERISLRQQVNNHFSAIITAAVP 151

Query: 174 --------------------------------------------------LLSLYEQGAR 233
                                                             L  LY  GAR
Sbjct: 152 LSRLRQCLYTINIGSNDYLNNYFLSPPTLARRLFNPDQYARSLISLYRIYLTQLYVLGAR 211

Query: 234 KVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVEDLNTQFPDAKFIF 293
            VA+FG+G IGCTP   A     G  C +++N AV +FN  LK+LV D N + P A F +
Sbjct: 212 NVALFGIGKIGCTPRIVATL-GGGTGCAEEVNQAVIIFNTKLKALVTDFNNK-PGAMFTY 271

Query: 294 VDVFQISTIQPSNFGKMVLEEPCCEVLPGQLQCSPFGKVCGDRSQYLFWDGVHPTEIGFT 303
           VD+F  +    +  G  V +  CC V PG+  C+  G VC DR++++FWD VH TE+  T
Sbjct: 272 VDLFSGNAEDFAALGITVGDRSCCTVNPGEELCAANGPVCPDRNKFIFWDNVHTTEVINT 331

BLAST of Spg003161 vs. ExPASy TrEMBL
Match: A0A0A0LKR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093840 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.5e-98
Identity = 186/346 (53.76%), Postives = 210/346 (60.69%), Query Frame = 0

Query: 45  RGVDGKSILPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDFSKQQT---PTGRF 104
           +G  G+ I  CYFVFGDSQADNGNNN +     G ARADYKPYGIDFS   +   PTGRF
Sbjct: 37  KGGSGRGICSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFSSSSSSYIPTGRF 96

Query: 105 TNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLL-------- 164
           TN RN+PDFIAKFLGF +YIPPF  TK   ILKG NYASGG+GIL ETGR L        
Sbjct: 97  TNARNVPDFIAKFLGFDDYIPPFRTTKSRTILKGANYASGGAGILRETGRTLGQVSSIKK 156

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 157 QLENHNYTISQMHTLLGSSSKINRYLKECLYTVQIGSNDYLNNYFMPKIYMTSTQYSPQA 216

Query: 225 --------------SLYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLF 284
                         +LY  GARKVA+FGVGSIGCTPYAR NFEH G PCVD+INSA+QLF
Sbjct: 217 FATALNKNLSQHLKNLYNYGARKVAIFGVGSIGCTPYARENFEHTGLPCVDEINSAIQLF 276

Query: 285 NIGLKSLVEDLNTQFPDAKFIFVDVFQISTIQPSNFGKMVLEEPCCEVLPGQLQCSPFGK 303
           N GLKSLV+ LN   P AKF F+DVFQIST+ P N+GKMVL+ PCCEV  G +QCSPFGK
Sbjct: 277 NSGLKSLVQHLNANLPSAKFTFIDVFQISTVDPLNYGKMVLDAPCCEVGAGAMQCSPFGK 336

BLAST of Spg003161 vs. ExPASy TrEMBL
Match: A0A6J1CSH4 (GDSL esterase/lipase At1g29670-like OS=Momordica charantia OX=3673 GN=LOC111014175 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 9.8e-95
Identity = 180/340 (52.94%), Postives = 216/340 (63.53%), Query Frame = 0

Query: 50  KSILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQ--TPTGRFTNGRNIPDF 109
           KS + CYF+FGDSQ+DNGNNN +  G A A+YKPYGIDF  Q   TPTGRFTNGRN+PDF
Sbjct: 55  KSRMDCYFIFGDSQSDNGNNNDIK-GDAMANYKPYGIDFPNQNSPTPTGRFTNGRNLPDF 114

Query: 110 IAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLL----------------- 169
           IA+ LGF +YIPPF NT   ++LKGVNYASGGSGI  ETGR+L                 
Sbjct: 115 IAELLGFHDYIPPFRNTTGWNVLKGVNYASGGSGIHSETGRMLGKVLSINKQLENHNHTI 174

Query: 170 ------------------------------------------------------------ 229
                                                                       
Sbjct: 175 SRIQGSLGSKLAATKHLKSCLYTVQAGSNDYLNNYFLPFSYKTSAQFTPQQYAVALNKQL 234

Query: 230 -----SLYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVE 289
                 LY++G RKVAVFGVG IGCTPYARANFEH+G+PCV+KIN A+QLFN GLKSL+ 
Sbjct: 235 SHQLEELYDRGGRKVAVFGVGGIGCTPYARANFEHEGSPCVEKINYAIQLFNSGLKSLIH 294

Query: 290 DLNTQFPDAKFIFVDVFQISTIQPSNF---GKMVLEEPCCEVLPGQLQCSPFGKVCGDRS 303
           DLNT+F DAKFIF+DVF+IST++P+       +VL+ PCCEV  GQLQC+PFGKVCG+RS
Sbjct: 295 DLNTKFTDAKFIFIDVFKISTMKPTPLVPADLLVLDAPCCEVPVGQLQCTPFGKVCGNRS 354

BLAST of Spg003161 vs. ExPASy TrEMBL
Match: A0A5D3DKM8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold419G00010 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.8e-94
Identity = 182/348 (52.30%), Postives = 208/348 (59.77%), Query Frame = 0

Query: 45  RGVDGKSILPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDF-----SKQQTPTG 104
           +G  G     CYFVFGDSQADNGNNN +     G ARADYKPYGIDF     S    PTG
Sbjct: 36  KGGGGSDACSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTG 95

Query: 105 RFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS----- 164
           RFTNGRN+PDFIA+FLGF +YIPPF  TK   ILKG NYASGG+GIL ETGR L      
Sbjct: 96  RFTNGRNVPDFIAQFLGFRDYIPPFRTTKSQTILKGANYASGGAGILLETGRTLGEVSSI 155

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 156 KKQLENHNYTISQMQRLFGSSSKLKSYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSP 215

Query: 225 -----------------LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQ 284
                            LYEQGARKVA+FGVGSIGCTPYARANFEH G PCVDKIN+A+Q
Sbjct: 216 QAFATALNEDLSRQLKILYEQGARKVAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQ 275

Query: 285 LFNIGLKSLVEDLNTQFPDAKFIFVDVFQISTIQPSNFGKMVLEEPCCEVLPGQLQCSPF 303
           LFN GLKSLVE LN   P AKF F+DVF+IST+ P N+GK+ L+ PCCEV  G++QC+PF
Sbjct: 276 LFNAGLKSLVEHLNANLPSAKFTFIDVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPF 335

BLAST of Spg003161 vs. ExPASy TrEMBL
Match: A0A1S3B473 (GDSL esterase/lipase At1g29670-like OS=Cucumis melo OX=3656 GN=LOC103485821 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.8e-94
Identity = 182/348 (52.30%), Postives = 208/348 (59.77%), Query Frame = 0

Query: 45  RGVDGKSILPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDF-----SKQQTPTG 104
           +G  G     CYFVFGDSQADNGNNN +     G ARADYKPYGIDF     S    PTG
Sbjct: 36  KGGGGSDACSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTG 95

Query: 105 RFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS----- 164
           RFTNGRN+PDFIA+FLGF +YIPPF  TK   ILKG NYASGG+GIL ETGR L      
Sbjct: 96  RFTNGRNVPDFIAQFLGFRDYIPPFRTTKSQTILKGANYASGGAGILLETGRTLGEVSSI 155

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 156 KKQLENHNYTISQMQRLFGSSSKLKSYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSP 215

Query: 225 -----------------LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQ 284
                            LYEQGARKVA+FGVGSIGCTPYARANFEH G PCVDKIN+A+Q
Sbjct: 216 QAFATALNEDLSRQLKILYEQGARKVAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQ 275

Query: 285 LFNIGLKSLVEDLNTQFPDAKFIFVDVFQISTIQPSNFGKMVLEEPCCEVLPGQLQCSPF 303
           LFN GLKSLVE LN   P AKF F+DVF+IST+ P N+GK+ L+ PCCEV  G++QC+PF
Sbjct: 276 LFNAGLKSLVEHLNANLPSAKFTFIDVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPF 335

BLAST of Spg003161 vs. ExPASy TrEMBL
Match: A0A6J1CPC1 (GDSL esterase/lipase At1g29670-like OS=Momordica charantia OX=3673 GN=LOC111013501 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 4.5e-92
Identity = 179/358 (50.00%), Postives = 218/358 (60.89%), Query Frame = 0

Query: 31  LMVYCFLTIAAFGTRGVDG--KSILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDF 90
           +M+Y  L    F T   +G   S + C+F+FGDSQ+DNGNNN  N G ARADYKPYGIDF
Sbjct: 10  IMLYYALISTYFSTYLANGASSSEVGCFFIFGDSQSDNGNNN-NNTGFARADYKPYGIDF 69

Query: 91  SKQQTPTGRFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGR 150
           SK+  PTGRFTNGRN+ DFI +FLGF NY+PPF+N    +ILKGVNYASG +GI  ETGR
Sbjct: 70  SKEMKPTGRFTNGRNVADFIVEFLGFDNYVPPFKNMSGWNILKGVNYASGAAGIRDETGR 129

Query: 151 ------------------------------------------------------------ 210
                                                                       
Sbjct: 130 TQGERSSFNKQLKNHNLIISKLQHLLGNRIAAEEHLKSCLYMVQIGGNDYLNNYFMPLSY 189

Query: 211 ----------------------LLSLYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCV 270
                                 L +LY QGARK+A+FG G IGCTPYARANF+ KG+PCV
Sbjct: 190 KTSTEFTPHQYATALSKQLSCKLKALYGQGARKLAIFGAGPIGCTPYARANFDTKGSPCV 249

Query: 271 DKINSAVQLFNIGLKSLVEDLNTQFPDAKFIFVDVFQIST--IQPSNFGKMVLEEPCCEV 303
           ++IN+A+QLFNIGLKS+V+DLNT+  +AKFIFVDVF I T     SN G+MV++ PCCEV
Sbjct: 250 EEINNAIQLFNIGLKSMVDDLNTKLTNAKFIFVDVFNIGTHGTPTSNQGEMVVDSPCCEV 309

BLAST of Spg003161 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 2.3e-56
Identity = 132/365 (36.16%), Postives = 173/365 (47.40%), Query Frame = 0

Query: 27  MKAQLMVYC-FLTIAAFGTRGV--DGKSILPCYFVFGDSQADNGNNNILNIGMARADYKP 86
           M++ L  +C  L +  FG   V    ++ +PC+FVFGDS  DNGNNN L I +AR++Y P
Sbjct: 1   MESYLTKWCVVLVLLCFGFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGL-ISIARSNYFP 60

Query: 87  YGIDFSKQQTPTGRFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGIL 146
           YGIDF     PTGRF+NG+   D IA+ LGF  YIP +       IL GVNYAS  +GI 
Sbjct: 61  YGIDFG---GPTGRFSNGKTTVDVIAELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIR 120

Query: 147 PETGRLL----------------------------------------------------- 206
            ETGR L                                                     
Sbjct: 121 EETGRQLGQRISFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYF 180

Query: 207 -----------------------------SLYEQGARKVAVFGVGSIGCTPYARANFEHK 266
                                        +LY  GARK A+ G+G++GC+P A A     
Sbjct: 181 MPTFYSSSRQFTPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALAG-SPD 240

Query: 267 GAPCVDKINSAVQLFNIGLKSLVEDLNTQFPDAKFIFVD---VFQISTIQPSNFGKMVLE 302
           G  CVD+INSA Q+FN  L+SLV+ LN   PDAKFI+++   +FQ     P+ FG  V  
Sbjct: 241 GRTCVDRINSANQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTN 300

BLAST of Spg003161 vs. TAIR 10
Match: AT4G18970.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 206.5 bits (524), Expect = 3.1e-53
Identity = 119/340 (35.00%), Postives = 155/340 (45.59%), Query Frame = 0

Query: 49  GKSILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNIPDFI 108
           G  I PCYF+FGDS  D+GNNN L   +ARA+Y PYGIDF  Q  PTGRF+NG+   D I
Sbjct: 22  GDPIAPCYFIFGDSLVDSGNNNRLT-SLARANYFPYGIDF--QYGPTGRFSNGKTTVDVI 81

Query: 109 AKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS----------------- 168
            + LGF +YI P+   +  DIL+GVNYAS  +GI  ETGR L                  
Sbjct: 82  TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVS 141

Query: 169 ------------------------------------------------------------ 228
                                                                       
Sbjct: 142 QVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYT 201

Query: 229 -----LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVED 288
                +Y  GARK A+ G+G+IGC+P   A     G  C ++INSA ++FN  L SLV+ 
Sbjct: 202 EQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDH 261

Query: 289 LNTQFPDAKFIFVD---VFQISTIQPSNFGKMVLEEPCCEV--LPGQLQCSPFGKVCGDR 302
            N   P AKF +++   +FQ     PS +G  V    CC V    GQ+ C P    C +R
Sbjct: 262 FNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNR 321

BLAST of Spg003161 vs. TAIR 10
Match: AT4G18970.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 206.5 bits (524), Expect = 3.1e-53
Identity = 119/340 (35.00%), Postives = 155/340 (45.59%), Query Frame = 0

Query: 49  GKSILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNIPDFI 108
           G  I PCYF+FGDS  D+GNNN L   +ARA+Y PYGIDF  Q  PTGRF+NG+   D I
Sbjct: 22  GDPIAPCYFIFGDSLVDSGNNNRLT-SLARANYFPYGIDF--QYGPTGRFSNGKTTVDVI 81

Query: 109 AKFLGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS----------------- 168
            + LGF +YI P+   +  DIL+GVNYAS  +GI  ETGR L                  
Sbjct: 82  TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVS 141

Query: 169 ------------------------------------------------------------ 228
                                                                       
Sbjct: 142 QVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYT 201

Query: 229 -----LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVED 288
                +Y  GARK A+ G+G+IGC+P   A     G  C ++INSA ++FN  L SLV+ 
Sbjct: 202 EQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDH 261

Query: 289 LNTQFPDAKFIFVD---VFQISTIQPSNFGKMVLEEPCCEV--LPGQLQCSPFGKVCGDR 302
            N   P AKF +++   +FQ     PS +G  V    CC V    GQ+ C P    C +R
Sbjct: 262 FNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNR 321

BLAST of Spg003161 vs. TAIR 10
Match: AT1G29660.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-52
Identity = 123/366 (33.61%), Postives = 168/366 (45.90%), Query Frame = 0

Query: 27  MKAQLMVYCFLTI----AAFGTRGVDGKSILPCYFVFGDSQADNGNNNILNIGMARADYK 86
           M++ L  +C +++       G + V  +  +PCYF+FGDS  DNGNNN L   +ARADY 
Sbjct: 1   MESYLRKWCLVSVWVLLLGLGFK-VKAEPQVPCYFIFGDSLVDNGNNNRLR-SIARADYF 60

Query: 87  PYGIDFSKQQTPTGRFTNGRNIPDFIAKFLGFVNYIPPFENTKDSDILKGVNYASGGSGI 146
           PYGIDF     PTGRF+NGR   D + + LGF NYIP +      +IL+GVNYAS  +GI
Sbjct: 61  PYGIDFG---GPTGRFSNGRTTVDVLTELLGFDNYIPAYSTVSGQEILQGVNYASAAAGI 120

Query: 147 LPETG------------------------------------------------------- 206
             ETG                                                       
Sbjct: 121 REETGAQLGQRITFSGQVENYKNTVAQVVEILGDEYTAADYLKRCIYSVGMGSNDYLNNY 180

Query: 207 ---------------------------RLLSLYEQGARKVAVFGVGSIGCTPYARANFEH 266
                                      +L +LY  GARK A+ G+G+IGC+P A A    
Sbjct: 181 FMPQFYSTSRQYTPEQYADDLISRYRDQLNALYNYGARKFALVGIGAIGCSPNALAQGSQ 240

Query: 267 KGAPCVDKINSAVQLFNIGLKSLVEDLNTQFPDAKFIFVD---VFQISTIQPSNFGKMVL 302
            G  CV++INSA ++FN  L S+V+ LN    DA F +++    FQ     PS +G    
Sbjct: 241 DGTTCVERINSANRIFNNRLISMVQQLNNAHSDASFTYINAYGAFQDIIANPSAYGFTNT 300

BLAST of Spg003161 vs. TAIR 10
Match: AT5G45670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-52
Identity = 119/337 (35.31%), Postives = 156/337 (46.29%), Query Frame = 0

Query: 52  ILPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNIPDFIAKF 111
           I PCYF+FGDS  DNGNNN L   +ARA+Y PYGIDF+    PTGRF+NG    D IA+ 
Sbjct: 26  IAPCYFIFGDSLVDNGNNNQLQ-SLARANYFPYGIDFA--AGPTGRFSNGLTTVDVIAQL 85

Query: 112 LGFVNYIPPFENTKDSDILKGVNYASGGSGILPETGRLLS-------------------- 171
           LGF +YI P+ + +  DIL+GVNYAS  +GI  ETGR L                     
Sbjct: 86  LGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVV 145

Query: 172 ------------------------------------------------------------ 231
                                                                       
Sbjct: 146 NILGDQNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQL 205

Query: 232 --LYEQGARKVAVFGVGSIGCTPYARANFEHKGAPCVDKINSAVQLFNIGLKSLVEDLNT 291
             LY  GARK A+ GVG+IGC+P   A     G  C ++INSA ++FN  L S+V+  N 
Sbjct: 206 RVLYTNGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQ 265

Query: 292 QFPDAKFIFVD---VFQISTIQPSNFGKMVLEEPCCEV--LPGQLQCSPFGKVCGDRSQY 302
             PDAKF +++   +FQ     P+ +G  V    CC V    GQ+ C P    C +R++Y
Sbjct: 266 NTPDAKFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEY 325