Spg003077 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003077
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450, family 706, subfamily A, polypeptide 3
Location	scaffold12: 38560430 .. 38561431 (-)
RNA-Seq Expression	Spg003077
Synteny	Spg003077

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCCACCTCCAATTTCCTTCAAATTAGTCTCTTTGATTCTTTAAGTTTCTTGTTTCCTTGGCGGTGGATTGACAATGGCTATTACCAATCGCTTTTTCTTTCCACGTTCTTTGCTGGTTTTCTAGTTTTTCTTTACACTAAAACTCGGCGGCGCTGCTTGGCGGCGCCACCGCCGCCGGGTCCACGTGGGATTCCGCTACTCGGAAATCTTCCATTTATGAAACCAGAACTCCACACTTATTTTACCGAACTCGGTCAGAAATACGGCCCAATCGTAAAGCTCCAACTTGGTAGCAAGATTTGTATAGTCGTGAACTCGGCCTCTGTGGCACGTGAAGTTCTCAAGGACCACGACCTCACATTCGCCAACCGCGACGTTCCTTTCGTCGGCAAAGCCTCTTCCTACGGTGGCTGCGACATACTTTGGAGCCCCTATGGACCCGAATGGCGGATGTTGAGAAAAATAAGCATGGTAAAAATGCTTAGCAACGCCGCTTTGGACTCCGTTTGTGAACTTCGACGGAGAGAGGTTAGAAACACCGTGCGTTATTTTTATAGTCAAATTGGGTGTCCGATTGATGTAGGAGAGCAAAGTTTTGTGACGCTGCTTAATGTCTTTACTGCCATGCTTTGGGGTAGGACGGTGGACGGAGAAGAGAGGAAGAGCATTGGGGTGGAGCTGAGAGAGGCGGCGGCGGAGATGGTGGACTTATTGATGAAACCTAATGTCTCTGACTTCTTTCCGAGATTGGCGCGTTTCGATATCCAAGGAATCGAGAAGCGAATGCTTCAATTATTCCCAAGATTTGACAATATCTTTACCAAAATGATCGATAAAAGATTGGAAATTGCCAATAAGGGTGGCGGTGCAAGTGCACAAGAAAAGGATTTCTTGCAGTTCTTGCTTGAGAAGAAAGATGAAGGAGATTCCAAAACTCCTTTCACCTCCAACCATCTTAAAGCCTTGCTCATGGTAATCAATCAGAGTACTAGTTAA

mRNA sequence

ATGGAACCCACCTCCAATTTCCTTCAAATTAGTCTCTTTGATTCTTTAAGTTTCTTGTTTCCTTGGCGGTGGATTGACAATGGCTATTACCAATCGCTTTTTCTTTCCACGTTCTTTGCTGGTTTTCTAGTTTTTCTTTACACTAAAACTCGGCGGCGCTGCTTGGCGGCGCCACCGCCGCCGGGTCCACGTGGGATTCCGCTACTCGGAAATCTTCCATTTATGAAACCAGAACTCCACACTTATTTTACCGAACTCGGTCAGAAATACGGCCCAATCGTAAAGCTCCAACTTGGTAGCAAGATTTGTATAGTCGTGAACTCGGCCTCTGTGGCACGTGAAGTTCTCAAGGACCACGACCTCACATTCGCCAACCGCGACGTTCCTTTCGTCGGCAAAGCCTCTTCCTACGGTGGCTGCGACATACTTTGGAGCCCCTATGGACCCGAATGGCGGATGTTGAGAAAAATAAGCATGGTAAAAATGCTTAGCAACGCCGCTTTGGACTCCGTTTGTGAACTTCGACGGAGAGAGGTTAGAAACACCGTGCGTTATTTTTATAGTCAAATTGGGTGTCCGATTGATGTAGGAGAGCAAAGTTTTGTGACGCTGCTTAATGTCTTTACTGCCATGCTTTGGGGTAGGACGGTGGACGGAGAAGAGAGGAAGAGCATTGGGGTGGAGCTGAGAGAGGCGGCGGCGGAGATGGTGGACTTATTGATGAAACCTAATGTCTCTGACTTCTTTCCGAGATTGGCGCGTTTCGATATCCAAGGAATCGAGAAGCGAATGCTTCAATTATTCCCAAGATTTGACAATATCTTTACCAAAATGATCGATAAAAGATTGGAAATTGCCAATAAGGGTGGCGGTGCAAGTGCACAAGAAAAGGATTTCTTGCAGTTCTTGCTTGAGAAGAAAGATGAAGGAGATTCCAAAACTCCTTTCACCTCCAACCATCTTAAAGCCTTGCTCATGGTAATCAATCAGAGTACTAGTTAA

Coding sequence (CDS)

ATGGAACCCACCTCCAATTTCCTTCAAATTAGTCTCTTTGATTCTTTAAGTTTCTTGTTTCCTTGGCGGTGGATTGACAATGGCTATTACCAATCGCTTTTTCTTTCCACGTTCTTTGCTGGTTTTCTAGTTTTTCTTTACACTAAAACTCGGCGGCGCTGCTTGGCGGCGCCACCGCCGCCGGGTCCACGTGGGATTCCGCTACTCGGAAATCTTCCATTTATGAAACCAGAACTCCACACTTATTTTACCGAACTCGGTCAGAAATACGGCCCAATCGTAAAGCTCCAACTTGGTAGCAAGATTTGTATAGTCGTGAACTCGGCCTCTGTGGCACGTGAAGTTCTCAAGGACCACGACCTCACATTCGCCAACCGCGACGTTCCTTTCGTCGGCAAAGCCTCTTCCTACGGTGGCTGCGACATACTTTGGAGCCCCTATGGACCCGAATGGCGGATGTTGAGAAAAATAAGCATGGTAAAAATGCTTAGCAACGCCGCTTTGGACTCCGTTTGTGAACTTCGACGGAGAGAGGTTAGAAACACCGTGCGTTATTTTTATAGTCAAATTGGGTGTCCGATTGATGTAGGAGAGCAAAGTTTTGTGACGCTGCTTAATGTCTTTACTGCCATGCTTTGGGGTAGGACGGTGGACGGAGAAGAGAGGAAGAGCATTGGGGTGGAGCTGAGAGAGGCGGCGGCGGAGATGGTGGACTTATTGATGAAACCTAATGTCTCTGACTTCTTTCCGAGATTGGCGCGTTTCGATATCCAAGGAATCGAGAAGCGAATGCTTCAATTATTCCCAAGATTTGACAATATCTTTACCAAAATGATCGATAAAAGATTGGAAATTGCCAATAAGGGTGGCGGTGCAAGTGCACAAGAAAAGGATTTCTTGCAGTTCTTGCTTGAGAAGAAAGATGAAGGAGATTCCAAAACTCCTTTCACCTCCAACCATCTTAAAGCCTTGCTCATGGTAATCAATCAGAGTACTAGTTAA

Protein sequence

MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQSLFLSTFFAGFLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLEKKDEGDSKTPFTSNHLKALLMVINQSTS

Homology

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BLAST of Spg003077 vs. NCBI nr
Match: KAG6579436.1 (Premnaspirodiene oxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 437.2 bits (1123), Expect = 1.3e-118
Identity = 216/328 (65.85%), Postives = 258/328 (78.66%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQSLFLST-FFAGFLVFLYTKTRRRCLAAPP 60
           MEPTSN LQ  + DSL+FL PWRWID    QS FL T FFAG L+FLYTK  RR    P 
Sbjct: 1   MEPTSNSLQTRI-DSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRR--RVPL 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNLPF+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKD+
Sbjct: 61  PPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDN 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A++YGG DI W+PYG EWRMLRK+ +VKMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQSF+T+ NV T+MLWG TV G++R S+  E RE  +++ +L
Sbjct: 181 RNTVAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+IA    G SA+ KDF
Sbjct: 241 LGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLMV 328
           LQFLLE KD GDSKTP T  HLKALLM+
Sbjct: 301 LQFLLEVKDSGDSKTPLTITHLKALLMM 325

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BLAST of Spg003077 vs. NCBI nr
Match: XP_022970206.1 (geraniol 8-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 434.5 bits (1116), Expect = 8.3e-118
Identity = 214/327 (65.44%), Postives = 257/327 (78.59%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQS-LFLSTFFAGFLVFLYTKTRRRCLAAPP 60
           MEPTSN LQI   DSL+FL PWRWID    QS L  + FFAG L+FLYTK  RR    P 
Sbjct: 1   MEPTSNPLQI-FIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRR--RVPL 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNLPF+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKD+
Sbjct: 61  PPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDN 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A+SYGG DI W+PYG EWRMLRK+ ++KMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAASYGGSDIAWTPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQSF+T+ NV T+MLWG TV+G++R S+  E RE  +++ +L
Sbjct: 181 RNTVAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+I+    G SA+ KDF
Sbjct: 241 LGKPNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKISGGEDGESAKNKDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLM 327
           LQFLLE KD GDSKTP T  HLKALLM
Sbjct: 301 LQFLLEVKDSGDSKTPLTITHLKALLM 324

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BLAST of Spg003077 vs. NCBI nr
Match: KAG6579434.1 (Angelicin synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429.1 bits (1102), Expect = 3.5e-116
Identity = 211/329 (64.13%), Postives = 254/329 (77.20%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQS-LFLSTFFAGFLVFLYTKTRRRCLAAPP 60
           ME TSN LQ +L DSL+FL PWRWID    QS L  + FFAG L+FLYTK +RR    P 
Sbjct: 1   METTSNSLQ-TLIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVKRR--RVPL 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNLPF+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKDH
Sbjct: 61  PPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDH 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A++YGG +I+W+PYG EWRMLRK+ +VKMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAATYGGSNIVWNPYGSEWRMLRKVCVVKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQ F+T+ NV T MLWG  ++G++R S+  E RE  ++  +L
Sbjct: 181 RNTVANLYGRAGSAVNVGEQGFLTVFNVVTGMLWGGIMEGDQRDSLAAEFRETVSDFTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+I     G SAQ  DF
Sbjct: 241 LGKPNVSDFFPSLARFDLQGIEKKMRKLALKFDTIFENMINQRLKIVGGEDGQSAQNHDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLMVI 329
           LQFLLE KD GDSKTP T  HLKALLM I
Sbjct: 301 LQFLLEMKDAGDSKTPLTMIHLKALLMAI 326

[Close]

BLAST of Spg003077 vs. NCBI nr
Match: XP_023551603.1 (geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.6 bits (1098), Expect = 1.0e-115
Identity = 211/327 (64.53%), Postives = 255/327 (77.98%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQS-LFLSTFFAGFLVFLYTKTRRRCLAAPP 60
           ME TSN LQ +L DSL+FL PWRWI     QS L  + FFAG L+FLYTK  RR +    
Sbjct: 1   METTSNSLQ-TLIDSLNFLLPWRWIHAADNQSRLLFTVFFAGLLIFLYTKVTRRRVTL-- 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNLPF+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKDH
Sbjct: 61  PPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDH 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A++YGG DI W+PYG EWRMLRK+ +VKMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAATYGGSDIAWTPYGAEWRMLRKVCVVKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQSF+T+ NV T+MLWG  ++G++R S+  E RE  +++ +L
Sbjct: 181 RNTVANLYGRGGSAVNVGEQSFLTIFNVVTSMLWGGIMEGDQRDSLAAEFRETVSDLTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+IA    G SA+ KDF
Sbjct: 241 LGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKSKDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLM 327
           LQFLLE KD GDSKTP T  HLKALLM
Sbjct: 301 LQFLLEVKDSGDSKTPLTITHLKALLM 324

[Close]

BLAST of Spg003077 vs. NCBI nr
Match: XP_022970207.1 (geraniol 8-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 426.8 bits (1096), Expect = 1.7e-115
Identity = 211/327 (64.53%), Postives = 256/327 (78.29%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQSLFLST-FFAGFLVFLYTKTRRRCLAAPP 60
           ME TSN L+ +L DSL+FL PW WID   YQ  FL T FFAG L+FLYTK  RR    P 
Sbjct: 1   METTSNSLR-TLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRR--RVPL 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNL F+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKD+
Sbjct: 61  PPGPLGVPLLGNLLFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDN 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A+ YGG +I+W+PYG EWRMLRK+ ++KMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAARYGGSNIVWNPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQSF+T+ NV T+MLWG TV+G++R S+  E RE  +++ +L
Sbjct: 181 RNTVAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+IA    G SA+ KDF
Sbjct: 241 LGKPNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLM 327
           LQFLLE KD GDSKTP T  HLKALLM
Sbjct: 301 LQFLLEVKDSGDSKTPLTITHLKALLM 324

[Close]

BLAST of Spg003077 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.0e-38
Identity = 102/317 (32.18%), Postives = 164/317 (51.74%), Query Frame = 0


Query: 16  LSFLFPWRWIDNGYYQSLFLSTFFAGFLVFLYTKTRRRCLAAPP-PPGPRGIPLLGNLPF 75
           L+F F W         +L LS+F       +Y   RRR    PP PPGP G P+LGNLP 
Sbjct: 2   LTFFFLW-------ISTLLLSSF------IVYLLYRRRSAQCPPLPPGPNGWPILGNLPQ 61

Query: 76  MKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKA 135
           +  + H     L ++YGP+ +L+LGS   +V +S++VA + L+ HD+ F+NR      + 
Sbjct: 62  LGAKPHQTLDALSKQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEH 121

Query: 136 SSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYF--YSQIGC 195
            +Y   D++++PYGP WRMLRK+  V + S  ALD +  +R+ EV   VR    ++  G 
Sbjct: 122 VAYNYQDLVFAPYGPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGV 181

Query: 196 PIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGV--ELREAAAEMVDLLMKPNVSDFFP 255
            +++G+   V   N     + GR V  +E   +    E +E   E++ L    NV DF P
Sbjct: 182 LVNLGKALNVCATNALARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVP 241

Query: 256 RLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLEKKD-E 315
            L   D+QG+  +M +L  R+D    ++I++          A+A+  D L  L+  K+ +
Sbjct: 242 GLGWLDLQGVVGKMKRLHRRYDAFLDRVIEE--------NQANAKSGDLLSVLIRLKEAD 297

Query: 316 GDSKTPFTSNHLKALLM 327
            + +    +  +KALL+
Sbjct: 302 AEGEIKLNNTDIKALLL 297

[Close]

BLAST of Spg003077 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 9.8e-37
Identity = 96/301 (31.89%), Postives = 153/301 (50.83%), Query Frame = 0


Query: 32  SLFLSTFFAGFLV-FLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKY 91
           SL L T    FL+ F+     R+    P PPGP+  P++GNL  + P+ H     + Q Y
Sbjct: 5   SLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQTY 64

Query: 92  GPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPE 151
           GP++ L++G    +V  SASVA + LK HD  F++R      +  +Y   D++++PYGP 
Sbjct: 65  GPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPR 124

Query: 152 WRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYFYSQIGCPIDVGEQSFVTLLNVFTA 211
           WRMLRKI  V + S  ALD    +R+ EV+   R   S    P+ +G+   V   N    
Sbjct: 125 WRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALAR 184

Query: 212 MLWGRTVDGEERKSIGVELREAAAEMVDLLMKP---NVSDFFPRLARFDIQGIEKRMLQL 271
           ++ G+ V  +    +  +  E  + +V++++     N+ DF P+L   DIQG+  +M +L
Sbjct: 185 VMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKL 244

Query: 272 FPRFDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLE-KKDEGDSK-TPFTSNHLKALL 327
             RFD   T ++++        G    + KD L  L+  K D+ D+     T   +KALL
Sbjct: 245 HARFDAFLTDILEEH------KGKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALL 299

[Close]

BLAST of Spg003077 vs. ExPASy Swiss-Prot
Match: P37120 (Flavonoid 3',5'-hydroxylase OS=Solanum melongena OX=4111 GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.8e-36
Identity = 86/278 (30.94%), Postives = 154/278 (55.40%), Query Frame = 0


Query: 51  RRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSAS 110
           RRR L    PPGP G P++G LP +    H    ++ +KYGPI+ L++G+   +V ++ +
Sbjct: 32  RRRRL----PPGPEGWPVIGALPLLGGMPHVALAKMAKKYGPIMYLKVGTCGMVVASTPN 91

Query: 111 VAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDS 170
            A+  LK  D+ F+NR         +Y   D++++PYGP W++LRK+S + ML   AL++
Sbjct: 92  AAKAFLKTLDINFSNRPPNAGATHMAYNAQDMVFAPYGPRWKLLRKLSNLHMLGGKALEN 151

Query: 171 VCELRRREVRNTVRYFY--SQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVE 230
              +R  E+ + ++  +  S +G  I V +     + N+   ++  + V  E+ K +  E
Sbjct: 152 WANVRANELGHMLKSMFDASHVGERIVVADMLTFAMANMIGQVMLSKRVFVEKGKEVN-E 211

Query: 231 LREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANK 290
            +    E++ +    N+ DF P++A  D+QGIEK M +L  +FD++ TKM ++    +N+
Sbjct: 212 FKNMVVELMTVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHKKFDDLLTKMFEEHEATSNE 271

Query: 291 GGGASAQEKDFLQFLLEKKDEGDSKTPFTSNHLKALLM 327
             G    + DFL F++  +D  + +    +N +KALL+
Sbjct: 272 RKG----KPDFLDFIMANRDNSEGERLSITN-IKALLL 299

[Close]

BLAST of Spg003077 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-35
Identity = 89/292 (30.48%), Postives = 151/292 (51.71%), Query Frame = 0


Query: 42  FLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSK 101
           F   L+ K  +     PP P P  +P++G+L  + P  H  F +L  +YGPI+ L LGS 
Sbjct: 16  FASILWRKQNKNKTLLPPSPMP--LPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSV 75

Query: 102 ICIVVNSASVAREVLKDHDLTFANRDVPFVG-KASSYGGCDILWSPYGPEWRMLRKISMV 161
            C+V ++A  A+E LK H+  F+NR    V  +  +YG  D L++PYGP W+ ++K+ M 
Sbjct: 76  PCVVASTAEAAKEFLKTHEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMS 135

Query: 162 KMLSNAALDSVCELRRREVRNTVRYFYSQ--IGCPIDVGEQSFVTLLNVFTAMLWGRTVD 221
           ++L    LD    +R+ E +  ++    +   G  +D G +      N+ + M+  +T  
Sbjct: 136 ELLGGHMLDQFLPVRQXETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTST 195

Query: 222 GEERKSIGVELREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKM 281
            E+   +  E+R+   +  +L  K N+SDF   L RFD+QG  KR+ ++   FD +  ++
Sbjct: 196 TEDENEV-EEMRKLVKDAAELSGKFNISDFVSFLKRFDLQGFNKRLEKIRDCFDTVLDRI 255

Query: 282 IDKR-LEIANKGGGASAQE-KDFLQFLLEKKDEGDSKTPFTSNHLKALLMVI 329
           I +R  E  NK      +E KD L  L +  ++  S+      ++KA ++ I
Sbjct: 256 IKQREEERRNKNETVGKREFKDMLDVLFDISEDESSEIKLNKENIKAFILDI 304

[Close]

BLAST of Spg003077 vs. ExPASy Swiss-Prot
Match: Q42799 (Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.1e-35
Identity = 89/291 (30.58%), Postives = 157/291 (53.95%), Query Frame = 0


Query: 42  FLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSK 101
           F   ++ K R++ L    PP P+G+P++G+L  + P  H  F +L  ++GPI++L LGS 
Sbjct: 16  FAYIVWRKERKKKL----PPSPKGLPIIGHLHLVSPIPHQDFYKLSLRHGPIMQLFLGSV 75

Query: 102 ICIVVNSASVAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVK 161
            C+V ++A  A+E LK H++ F+NR     G+  +      ++ PYGP  + ++K+ M +
Sbjct: 76  PCVVASTAEAAKEFLKTHEINFSNRP----GQNVAVQFLTYVFGPYGPSVKFIKKLCMSE 135

Query: 162 MLSNAALDSVCELRRREVRNTVRYFYSQ--IGCPIDVGEQSFVTLLNVFTAMLWGRTVDG 221
           +L    LD    +R++E +  ++    +   G  +D G +      N+ + M   +T   
Sbjct: 136 LLGGRMLDQFLPVRQQETKKFIKRVLQKGIAGEAVDFGGEFMRLSNNIISRMTMNQTSSE 195

Query: 222 EERKSIGVELREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMI 281
           +E+++   E+R   A++ +L+   NVSDF   L  FD+QG  KR+ +   RFD +  ++I
Sbjct: 196 DEKQA--EEMRMLVADVAELMGTFNVSDFIWFLKPFDLQGFNKRIRKTRIRFDAVLDRII 255

Query: 282 DKRLEIA--NKGGGASAQEKDFLQFLLEKKDEGDSKTPFTSNHLKALLMVI 329
            +R E    NK  G + Q KD L  LL+  ++  S+   T  ++KA +M I
Sbjct: 256 KQREEERRNNKEIGGTRQFKDILDVLLDIGEDDSSEIKLTKENIKAFIMDI 296

[Close]

BLAST of Spg003077 vs. ExPASy TrEMBL
Match: A0A6J1I001 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 4.0e-118
Identity = 214/327 (65.44%), Postives = 257/327 (78.59%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQS-LFLSTFFAGFLVFLYTKTRRRCLAAPP 60
           MEPTSN LQI   DSL+FL PWRWID    QS L  + FFAG L+FLYTK  RR    P 
Sbjct: 1   MEPTSNPLQI-FIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRR--RVPL 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNLPF+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKD+
Sbjct: 61  PPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDN 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A+SYGG DI W+PYG EWRMLRK+ ++KMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAASYGGSDIAWTPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQSF+T+ NV T+MLWG TV+G++R S+  E RE  +++ +L
Sbjct: 181 RNTVAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+I+    G SA+ KDF
Sbjct: 241 LGKPNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKISGGEDGESAKNKDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLM 327
           LQFLLE KD GDSKTP T  HLKALLM
Sbjct: 301 LQFLLEVKDSGDSKTPLTITHLKALLM 324

[Close]

BLAST of Spg003077 vs. ExPASy TrEMBL
Match: A0A6J1HYH5 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 8.4e-116
Identity = 211/327 (64.53%), Postives = 256/327 (78.29%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQSLFLST-FFAGFLVFLYTKTRRRCLAAPP 60
           ME TSN L+ +L DSL+FL PW WID   YQ  FL T FFAG L+FLYTK  RR    P 
Sbjct: 1   METTSNSLR-TLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRR--RVPL 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNL F+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKD+
Sbjct: 61  PPGPLGVPLLGNLLFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDN 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A+ YGG +I+W+PYG EWRMLRK+ ++KMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAARYGGSNIVWNPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQSF+T+ NV T+MLWG TV+G++R S+  E RE  +++ +L
Sbjct: 181 RNTVAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+IA    G SA+ KDF
Sbjct: 241 LGKPNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLM 327
           LQFLLE KD GDSKTP T  HLKALLM
Sbjct: 301 LQFLLEVKDSGDSKTPLTITHLKALLM 324

[Close]

BLAST of Spg003077 vs. ExPASy TrEMBL
Match: A0A6J1E5N3 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.4e-115
Identity = 210/327 (64.22%), Postives = 252/327 (77.06%), Query Frame = 0


Query: 1   MEPTSNFLQISLFDSLSFLFPWRWIDNGYYQS-LFLSTFFAGFLVFLYTKTRRRCLAAPP 60
           ME TSN LQ +L DSL+FL PWRWID    QS L  + FFAG L+FLYTK  RR    P 
Sbjct: 1   METTSNSLQ-TLIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRR--RVPL 60

Query: 61  PPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDH 120
           PPGP G+PLLGNLPF+ PELHTYF +LGQKYGPIVKLQLG+K+ I+VNS SVARE+LKDH
Sbjct: 61  PPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDH 120

Query: 121 DLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREV 180
           D+TFANRDVP  G+A++YGG +I+W+PYG EWRMLRK+ +VKMLSNA L+SV +LRRREV
Sbjct: 121 DVTFANRDVPQAGRAATYGGSNIVWNPYGSEWRMLRKVCVVKMLSNATLESVYDLRRREV 180

Query: 181 RNTVRYFYSQIGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDL 240
           RNTV   Y + G  ++VGEQ F+T+ NV T MLWG  ++G++R S+  E RE  ++  +L
Sbjct: 181 RNTVANLYGRAGSAVNVGEQGFLTVFNVVTGMLWGGIMEGDQRDSLAAEFRETVSDFTEL 240

Query: 241 LMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDF 300
           L KPNVSDFFP LARFD+QGIEK+M +L  +FD IF  MI++RL+I     G SAQ  DF
Sbjct: 241 LGKPNVSDFFPSLARFDLQGIEKKMRKLALKFDTIFENMINQRLKIVGGEDGQSAQNHDF 300

Query: 301 LQFLLEKKDEGDSKTPFTSNHLKALLM 327
           LQFLLE KD GDSKTP T  HLKALLM
Sbjct: 301 LQFLLEMKDAGDSKTPLTMIHLKALLM 324

[Close]

BLAST of Spg003077 vs. ExPASy TrEMBL
Match: A0A5A7T876 (Flavonoid 3'-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00040 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 3.1e-110
Identity = 195/296 (65.88%), Postives = 235/296 (79.39%), Query Frame = 0


Query: 31  QSLFLSTFFAGFLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKY 90
           QS F  TF A  L+FLY +  R  L  P PPGPRG+PLLGNLPF+ PELHTYF +L QKY
Sbjct: 13  QSHFFFTFLAALLIFLYLRLTR--LRVPLPPGPRGVPLLGNLPFLDPELHTYFAQLSQKY 72

Query: 91  GPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPE 150
           GPIVKLQLG KI I++NS SV REVLKDHD+TFANRDVP  G+A+SYGG DI+W+PYGP+
Sbjct: 73  GPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPHAGRAASYGGSDIVWTPYGPQ 132

Query: 151 WRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYFYSQIGCPIDVGEQSFVTLLNVFTA 210
           WRMLRK+ ++KMLSNA LDSV ELRRREVRNTV + Y++ G  ++VGEQ F+T+ NV T+
Sbjct: 133 WRMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHLYARAGTRVNVGEQGFLTVFNVVTS 192

Query: 211 MLWGRTVDGEERKSIGVELREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQLFPR 270
           MLWG +V+GE+R  +  E RE  +EM +LL  PNVSDFFP LARFDIQGIEK+M +L PR
Sbjct: 193 MLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDIQGIEKKMRELAPR 252

Query: 271 FDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLEKKDEGDSKTPFTSNHLKALLM 327
           FD+IF KMID+RL+I  K  G S ++ DFLQFLL+ KD+G+SKTP T  HLKALLM
Sbjct: 253 FDSIFEKMIDQRLKINGKDEGESVKKNDFLQFLLQVKDDGESKTPLTMTHLKALLM 306

[Close]

BLAST of Spg003077 vs. ExPASy TrEMBL
Match: A0A1S3ASY0 (flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 3.1e-110
Identity = 195/296 (65.88%), Postives = 235/296 (79.39%), Query Frame = 0


Query: 31  QSLFLSTFFAGFLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKY 90
           QS F  TF A  L+FLY +  R  L  P PPGPRG+PLLGNLPF+ PELHTYF +L QKY
Sbjct: 13  QSHFFFTFLAALLIFLYLRLTR--LRVPLPPGPRGVPLLGNLPFLDPELHTYFAQLSQKY 72

Query: 91  GPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPE 150
           GPIVKLQLG KI I++NS SV REVLKDHD+TFANRDVP  G+A+SYGG DI+W+PYGP+
Sbjct: 73  GPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPHAGRAASYGGSDIVWTPYGPQ 132

Query: 151 WRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYFYSQIGCPIDVGEQSFVTLLNVFTA 210
           WRMLRK+ ++KMLSNA LDSV ELRRREVRNTV + Y++ G  ++VGEQ F+T+ NV T+
Sbjct: 133 WRMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHLYARAGTRVNVGEQGFLTVFNVVTS 192

Query: 211 MLWGRTVDGEERKSIGVELREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQLFPR 270
           MLWG +V+GE+R  +  E RE  +EM +LL  PNVSDFFP LARFDIQGIEK+M +L PR
Sbjct: 193 MLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDIQGIEKKMRELAPR 252

Query: 271 FDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLEKKDEGDSKTPFTSNHLKALLM 327
           FD+IF KMID+RL+I  K  G S ++ DFLQFLL+ KD+G+SKTP T  HLKALLM
Sbjct: 253 FDSIFEKMIDQRLKIDGKDEGESVKKNDFLQFLLQVKDDGESKTPLTMTHLKALLM 306

[Close]

BLAST of Spg003077 vs. TAIR 10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3 )

HSP 1 Score: 306.6 bits (784), Expect = 2.4e-83
Identity = 151/285 (52.98%), Postives = 202/285 (70.88%), Query Frame = 0


Query: 44  VFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKYGPIVKLQLGSKIC 103
           ++LY K +RR  + P PPGP G+P++GNLPF++PELHTYF  L +K+GPI KL LG+K+ 
Sbjct: 33  LWLYAKCKRR--SPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPIFKLWLGAKLT 92

Query: 104 IVVNSASVAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPEWRMLRKISMVKML 163
           IVV S+ VA+E+LK +D+ FAN DVP VG  ++YGG +I+WSPYGP+WRMLRK+ + ++L
Sbjct: 93  IVVTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRMLRKLCVNRIL 152

Query: 164 SNAALDSVCELRRREVRNTVRYFYSQ--IGCPIDVGEQSFVTLLNVFTAMLWGRTVDGEE 223
            NA LDS  +LRRRE R TVRY   Q  +G P+++GEQ F+ +LNV T MLWG TV  EE
Sbjct: 153 RNAMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQMLWGTTVKEEE 212

Query: 224 RKSIGVELREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQLFPRFDNIFTKMIDK 283
           R+ +G E  E   EM DLL+ PN+SDFFP L+RFD+QG+ KRM +   R D +F ++I++
Sbjct: 213 REVVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRMDQMFDRIINQ 272

Query: 284 RLEIANKGGGASAQEKDFLQFLLEKKDEGDSKTPFTSNHLKALLM 327
           RL +        A   DFL  LL+ KDE   KT  T N +KA+LM
Sbjct: 273 RLGMDRDSSDGRA--VDFLDVLLKVKDEEAEKTKLTMNDVKAVLM 313

[Close]

BLAST of Spg003077 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 302.4 bits (773), Expect = 4.6e-82
Identity = 155/313 (49.52%), Postives = 212/313 (67.73%), Query Frame = 0


Query: 16  LSFLFPWRWIDNGYYQSLFLSTFFAGFLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFM 75
           +S LFP   I+   Y  + L+T F+  L +++ ++ +  L    PPGPRG+P++GNLPF+
Sbjct: 4   ISNLFPDNTINLTPYAIVILTTVFS-ILWYIFKRSPQPSL----PPGPRGLPIVGNLPFL 63

Query: 76  KPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKAS 135
            P+LHTYF  L Q +GPI KL LGSK+ IVVNS S+ARE+LKD D+ F+NRDVP  G+A+
Sbjct: 64  DPDLHTYFANLAQSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAA 123

Query: 136 SYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYFYSQ--IGCP 195
           +YGG DI+W+PYG EWR LRKI ++K+LS   LDS  ELRR+EVR   RY Y Q     P
Sbjct: 124 TYGGIDIVWTPYGAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSP 183

Query: 196 IDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDLLMKPNVSDFFPRLA 255
           + VG+Q F+T++N+   MLWG +V  EE +S+G E +   +E+  LL +PNVSDFFP LA
Sbjct: 184 VKVGDQLFLTMMNLTMNMLWGGSVKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLA 243

Query: 256 RFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLEKKD-EGDS 315
           RFD+QG+ KRM       D +  + I++   +    G    + KDFLQ+L++ KD EGDS
Sbjct: 244 RFDLQGLVKRMGVCARELDAVLDRAIEQMKPLR---GRDDDEVKDFLQYLMKLKDQEGDS 303

Query: 316 KTPFTSNHLKALL 326
           + P T NH+KALL
Sbjct: 304 EVPITINHVKALL 308

[Close]

BLAST of Spg003077 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 297.4 bits (760), Expect = 1.5e-80
Identity = 150/314 (47.77%), Postives = 213/314 (67.83%), Query Frame = 0


Query: 16  LSFLFPWRWIDNGYYQSLFLSTFFAGFLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFM 75
           +S LF    I+   Y  L L   F+  L +L+ ++ +  L    PPGPRG+P++GNLPF+
Sbjct: 4   ISNLFAGNTINLTPYAILILIAIFS-ILWYLFKRSPQPHL----PPGPRGLPIVGNLPFL 63

Query: 76  KPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKAS 135
            P+LHTYFT+L + YGPI KL LGSK+ +VVN+ S+ARE+LKD D+ F+N DVP   +A 
Sbjct: 64  DPDLHTYFTKLAESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAV 123

Query: 136 SYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYFY--SQIGCP 195
           +YGG D++W PYG EWRMLRK+ ++K+LS+  L+S  ELRR+E+R   RY Y   Q   P
Sbjct: 124 TYGGLDLVWLPYGAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESP 183

Query: 196 IDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDLLMKPNVSDFFPRLA 255
           ++VGEQ F+T++N+   MLWG +V  EE +S+G E +E  +E+  LL +PNVSDFFPRLA
Sbjct: 184 VNVGEQVFLTMMNLTMNMLWGGSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLA 243

Query: 256 RFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLEKKD-EGDS 315
           RFD+QG+ K+M       D I  + I+ ++++     G   + KDFLQ L++ KD E DS
Sbjct: 244 RFDLQGLVKKMHVCARELDAILDRAIE-QMQLLRTRDGDDGECKDFLQHLMKLKDQEADS 303

Query: 316 KTPFTSNHLKALLM 327
           + P T NH+KA+L+
Sbjct: 304 EVPITVNHVKAVLV 311

[Close]

BLAST of Spg003077 vs. TAIR 10
Match: AT4G12330.1 (cytochrome P450, family 706, subfamily A, polypeptide 7 )

HSP 1 Score: 295.0 bits (754), Expect = 7.3e-80
Identity = 153/302 (50.66%), Postives = 209/302 (69.21%), Query Frame = 0


Query: 32  SLFLSTFFAGFLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPFMKPELHTYFTELGQKYG 91
           ++ +S+ F  + +++Y K++R  L  P PPGPRG+P++GNLPF+ PELHTYF  L QK+G
Sbjct: 23  TIVISSVF--WYIWVYVKSKR--LFPPLPPGPRGLPIVGNLPFLHPELHTYFHSLAQKHG 82

Query: 92  PIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKASSYGGCDILWSPYGPEW 151
           P+ KL LG+K+ IV+ S+   R++L+ +D+ FAN DVP  G  S+YGG DI+WSPYGPEW
Sbjct: 83  PVFKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWSPYGPEW 142

Query: 152 RMLRKISMVKMLSNAALD--SVCELRRREVRNTVRYF--YSQIGCPIDVGEQSFVTLLNV 211
            MLRKI + KMLSNA LD  S   LRR+E R TVRY    ++ G  ++VGEQ FVT+LNV
Sbjct: 143 PMLRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIFVTILNV 202

Query: 212 FTAMLWGRTV-DGEERKSIGVELREAAAEMVDLLMKPNVSDFFPRLARFDIQGIEKRMLQ 271
            T MLWG TV D EER+ +G E  E   E++D++ KPNVSDFFP L+RFD+QG+ KR+ +
Sbjct: 203 VTQMLWGETVADDEEREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGLAKRVRR 262

Query: 272 LFPRFDNIFTKMIDKR--LEIANKGGGASAQEKDFLQFLLEKKDEGDSKTPFTSNHLKAL 327
              R D +F ++I +R  ++  +KG G      DFL  LL  KDE ++    + NH+KAL
Sbjct: 263 SAQRMDRMFDRIISQRMGMDKGSKGNGG-----DFLMVLLNAKDEDEN---MSMNHVKAL 312

[Close]

BLAST of Spg003077 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 282.3 bits (721), Expect = 4.9e-76
Identity = 145/315 (46.03%), Postives = 202/315 (64.13%), Query Frame = 0


Query: 16  LSFLFPWRWID-NGYYQSLFLSTFFAGFLVFLYTKTRRRCLAAPPPPGPRGIPLLGNLPF 75
           LS LFP   I    Y  ++ + T     L +++    +R    P PPGPRG+P++GNLPF
Sbjct: 4   LSNLFPDNAISLTPYAYAVLILTATFSILWYIF----KRSPQPPLPPGPRGLPIVGNLPF 63

Query: 76  MKPELHTYFTELGQKYGPIVKLQLGSKICIVVNSASVAREVLKDHDLTFANRDVPFVGKA 135
           + P+LHTYFT+L Q +GPI KL LGSK+ +VVNS S+A E+LKD D+ F+N DVP   +A
Sbjct: 64  LDPDLHTYFTKLAQSHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARA 123

Query: 136 SSYGGCDILWSPYGPEWRMLRKISMVKMLSNAALDSVCELRRREVRNTVRYFYSQ--IGC 195
            +YGG D++W PYG EWRMLRK+   K+ S   LDS  ELRR+E+R   R  Y +     
Sbjct: 124 VTYGGLDLVWLPYGAEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKS 183

Query: 196 PIDVGEQSFVTLLNVFTAMLWGRTVDGEERKSIGVELREAAAEMVDLLMKPNVSDFFPRL 255
           P++VGEQ F+T++N+   MLWG +V  E+ +S+G E +   +E+  LL  PNVSDFFP L
Sbjct: 184 PVNVGEQLFLTMMNLMMNMLWGGSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPML 243

Query: 256 ARFDIQGIEKRMLQLFPRFDNIFTKMIDKRLEIANKGGGASAQEKDFLQFLLEKKD-EGD 315
           ARFD+QG+ K+M       D I  + I++   + ++ G    + KDFLQ L++ +D E D
Sbjct: 244 ARFDLQGLVKKMHLYARDLDAILDRAIEQMQRLRSRDGD-DGECKDFLQHLMKLRDQEAD 303

Query: 316 SKTPFTSNHLKALLM 327
           S  P T NH+KA+LM
Sbjct: 304 SDVPITMNHVKAVLM 313

The following BLAST results are available for this feature:

BLAST of Spg003077 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6579436.1	1.3e-118	65.85	Premnaspirodiene oxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022970206.1	8.3e-118	65.44	geraniol 8-hydroxylase-like [Cucurbita maxima]	[more]
KAG6579434.1	3.5e-116	64.13	Angelicin synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023551603.1	1.0e-115	64.53	geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022970207.1	1.7e-115	64.53	geraniol 8-hydroxylase-like [Cucurbita maxima]	[more]

BLAST of Spg003077 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A4D6Q415	4.0e-38	32.18	Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN...	[more]
Q9SBQ9	9.8e-37	31.89	Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1	[more]
P37120	2.8e-36	30.94	Flavonoid 3',5'-hydroxylase OS=Solanum melongena OX=4111 GN=CYP75A2 PE=2 SV=1	[more]
O81973	1.1e-35	30.48	Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1	[more]
Q42799	3.1e-35	30.58	Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1	[more]

BLAST of Spg003077 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I001	4.0e-118	65.44	geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=...	[more]
A0A6J1HYH5	8.4e-116	64.53	geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=...	[more]
A0A6J1E5N3	1.4e-115	64.22	geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 S...	[more]
A0A5A7T876	3.1e-110	65.88	Flavonoid 3'-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A1S3ASY0	3.1e-110	65.88	flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=...	[more]

BLAST of Spg003077 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44620.1	2.4e-83	52.98	cytochrome P450, family 706, subfamily A, polypeptide 3	[more]
AT4G12300.1	4.6e-82	49.52	cytochrome P450, family 706, subfamily A, polypeptide 4	[more]
AT4G12320.1	1.5e-80	47.77	cytochrome P450, family 706, subfamily A, polypeptide 6	[more]
AT4G12330.1	7.3e-80	50.66	cytochrome P450, family 706, subfamily A, polypeptide 7	[more]
AT4G12310.1	4.9e-76	46.03	cytochrome P450, family 706, subfamily A, polypeptide 5	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 203..221 score: 18.86 coord: 111..132 score: 25.58 coord: 87..106 score: 32.36
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 60..327 e-value: 1.3E-31 score: 109.8
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 53..330 e-value: 4.6E-46 score: 159.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 60..327
None	No IPR available	PANTHER	PTHR47951:SF3	CYTOCHROME P450, FAMILY 706, SUBFAMILY A, POLYPEPTIDE 3-RELATED	coord: 33..326
None	No IPR available	PANTHER	PTHR47951	OS08G0547900 PROTEIN	coord: 33..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003077.1	Spg003077.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen