Spg003010 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003010
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	LINE-1 reverse transcriptase-like
Location	scaffold12: 37434945 .. 37435825 (-)
RNA-Seq Expression	Spg003010
Synteny	Spg003010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAAGACATCAATTATCGGGATTAACATAGACAAGTTAGAAATGGATGATTGGGCAAAGAACCTCGGATGTCAATGTGAAGCCCTCCCTATGAATTACCTTGGCTTTCCTATGGGTGGTAATTTTCGAAACAAGACCTTTTGGAATCCCTTGATTGAAAGATTCAACAGGAAGTTGGAAGTGTGGAAGAGATTCCCTATTTCAAAAGGTGGAAGAGTTACCCTTGTCCAAGCTACTCTTAATAGCCTCCCTTTATATTTTTTTCCCTCCTTAAAGCTCCATCCTCGGCCATAAAGGCCATGGAGAAAATCAGTAGAGATTTCATCTGGAATGGAGGAAATTATAAACTTGGCAGCAACCTTGTGAAATGGGAATGGACCTCCCTCCCTTTGAGTCATGGAGGTTTGGGGGTGGGGGCTCTTCGACAGAGGAATATAGCTCTCATCACCAAGTGGCTTTGGAGATTTTCCCAAGAGAAAGAAGCCTTGTGGAGGAAAGTTGTTGCTAGTATATATGGTGTGTCCTCTTTGGGGTGGAAGTCAGGACAACAAATCAAGAAAAAAGGAAATAGAATCTGGGCTGAAATTATCAAATTATACCCTGTTTTTGAACAATTTACAAATTTTGTTGTCTAGAATGGGGAGAACATTAGTTTTTTGGAGGACGTTTGGTGTGATAACCAGCACAACTAAAGCAAAGGTTCCCAGATATTTTGACCATCATAGACAGAAACGAAGCCACGCTGGGATTTGGGAAACTGCACTTGGAACCTTAGACTTTGCAGACGTTTGTTTGACAGGGAGCTTCCTAGCTGGGCTGGTTTAGTGAATATCCTGAATGACATTCACTTGTCAAATGATCAGGACAAGATTAGATAG

mRNA sequence

ATGGCTAAGACATCAATTATCGGGATTAACATAGACAAGTTAGAAATGGATGATTGGGCAAAGAACCTCGGATGTCAATGTGAAGCCCTCCCTATGAATTACCTTGGCTTTCCTATGGGTGCTCCATCCTCGGCCATAAAGGCCATGGAGAAAATCAGTAGAGATTTCATCTGGAATGGAGGAAATTATAAACTTGGCAGCAACCTTGTGAAATGGGAATGGACCTCCCTCCCTTTGAGTCATGGAGGTTTGGGGGTGGGGGCTCTTCGACAGAGGAATATAGCTCTCATCACCAAGTGGCTTTGGAGATTTTCCCAAGAGAAAGAAGCCTTGTGGAGGAAAGTTGTTGCTAGTATATATGAAACGAAGCCACGCTGGGATTTGGGAAACTGCACTTGGAACCTTAGACTTTGCAGACGTTTGTTTGACAGGGAGCTTCCTAGCTGGGCTGGTTTAGTGAATATCCTGAATGACATTCACTTGTCAAATGATCAGGACAAGATTAGATAG

Coding sequence (CDS)

ATGGCTAAGACATCAATTATCGGGATTAACATAGACAAGTTAGAAATGGATGATTGGGCAAAGAACCTCGGATGTCAATGTGAAGCCCTCCCTATGAATTACCTTGGCTTTCCTATGGGTGCTCCATCCTCGGCCATAAAGGCCATGGAGAAAATCAGTAGAGATTTCATCTGGAATGGAGGAAATTATAAACTTGGCAGCAACCTTGTGAAATGGGAATGGACCTCCCTCCCTTTGAGTCATGGAGGTTTGGGGGTGGGGGCTCTTCGACAGAGGAATATAGCTCTCATCACCAAGTGGCTTTGGAGATTTTCCCAAGAGAAAGAAGCCTTGTGGAGGAAAGTTGTTGCTAGTATATATGAAACGAAGCCACGCTGGGATTTGGGAAACTGCACTTGGAACCTTAGACTTTGCAGACGTTTGTTTGACAGGGAGCTTCCTAGCTGGGCTGGTTTAGTGAATATCCTGAATGACATTCACTTGTCAAATGATCAGGACAAGATTAGATAG

Protein sequence

MAKTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMGAPSSAIKAMEKISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKEALWRKVVASIYETKPRWDLGNCTWNLRLCRRLFDRELPSWAGLVNILNDIHLSNDQDKIR

Homology

[Close]

BLAST of Spg003010 vs. NCBI nr
Match: XP_022158566.1 (uncharacterized protein LOC111025019 [Momordica charantia])

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-25
Identity = 61/135 (45.19%), Postives = 80/135 (59.26%), Query Frame = 0


Query: 1   MAKTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMG---------------APSSA 60
           +AKTS+IGINI + E       +GC+ E +   YLG P+G               AP S 
Sbjct: 134 LAKTSLIGINIGENEATSKVDKIGCRVERMSFRYLGLPLGGNHRVIVFWRHLVEKAPVSI 193

Query: 61  IKAMEKISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFS 120
            K M KI RD  W+G       NLVKW+ +SLP+  GGLG+GA   +N AL+ KWLW F+
Sbjct: 194 TKEMNKIVRDLFWSGATSHGCKNLVKWDISSLPMKLGGLGIGAFPPKNNALLMKWLWHFT 253

[Close]

BLAST of Spg003010 vs. NCBI nr
Match: RVW39501.1 (microtubule-associated protein AIR9 [Vitis vinifera])

HSP 1 Score: 118.6 bits (296), Expect = 5.1e-23
Identity = 63/194 (32.47%), Postives = 95/194 (48.97%), Query Frame = 0


Query: 3   KTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMGAPSSA-------------IKAM 62
           K+SI GIN+D+  +   A+ L C+    P+ YLG P+G    A                +
Sbjct: 196 KSSIYGINLDQALLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVVERISMAAKI 255

Query: 63  EKISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKE 122
           E++ RDF+W+G       +LV+W+    P + GGLG+G +  RN+AL+ KWLWR+ +E  
Sbjct: 256 ERLQRDFLWSGFGEGKRDHLVRWDVVCKPKTIGGLGLGNISWRNLALLGKWLWRYPREGS 315

Query: 123 ALWRKVVASIYETKPR-WDLGNC--------------------TWNLRLCRRLFDRELPS 163
           ALW +V+ SIY +    WD                         WNL   R L D E+  
Sbjct: 316 ALWHQVILSIYGSHSNGWDANTLETGKEFGSGKICVLGPSRPFLWNLNFRRNLSDSEIED 375

[Close]

BLAST of Spg003010 vs. NCBI nr
Match: RVW36866.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 115.9 bits (289), Expect = 3.3e-22
Identity = 58/170 (34.12%), Postives = 89/170 (52.35%), Query Frame = 0


Query: 3   KTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMGA-----------PSSAIKAMEK 62
           K++I GIN+++  +   A+ L C+    P+ YLG P+G            P+S    +EK
Sbjct: 219 KSNIYGINLEQNHLSRLAEMLDCKASGWPILYLGLPLGGNPKTSGFWDPIPASVAAKIEK 278

Query: 63  ISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKEAL 122
           + RDF+W+G       +LV W+    P S GGLG G +  RN+AL+ KWLWR+ +E  AL
Sbjct: 279 MQRDFLWSGVGEGKRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSAL 338

Query: 123 WRKVVASIYETKPR-WDLGN-------CTWNLRLCRRLFDRELPSWAGLV 154
           W +V+ SIY +    WD+ N       C W  +    ++ +  PS+   V
Sbjct: 339 WHQVILSIYGSHSNGWDVNNIVRWSHRCPW--KAIALVYTKSFPSFTRFV 386

[Close]

BLAST of Spg003010 vs. NCBI nr
Match: RVX20623.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 115.5 bits (288), Expect = 4.3e-22
Identity = 56/129 (43.41%), Postives = 77/129 (59.69%), Query Frame = 0


Query: 9   INIDKLE---------MDDWAKNLGCQCEALPMNYLGFPMGAPSSAI-KAMEKISRDFIW 68
           IN++K E         MDD A+  GC   +LP  YLG P+GAP + + + +EKI RDF+W
Sbjct: 11  INLEKSELIPVGRVESMDDLAEEFGCSLGSLPTTYLGMPLGAPFNTVRRRLEKIQRDFLW 70

Query: 69  NGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKEALWRKVVAS 127
            GGN +   +LV+WE   L  + GGLGV +L   N  L+ KW WRF+ E+EALW +V+  
Sbjct: 71  GGGNLEHKPHLVRWELVCLSKAKGGLGVKSLSLLNKTLLAKWNWRFANEREALWNQVIRG 130

[Close]

BLAST of Spg003010 vs. NCBI nr
Match: RVW90069.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 112.1 bits (279), Expect = 4.8e-21
Identity = 63/212 (29.72%), Postives = 97/212 (45.75%), Query Frame = 0


Query: 3   KTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMGA-----------PSSAIKAMEK 62
           K++I GIN+D+  +   A+ L C+    P+ YLG P+G            P+S    +E+
Sbjct: 56  KSNIYGINLDQAHISRLAETLECKASGWPILYLGLPLGGNPRAGGFWDPLPASVAAKIER 115

Query: 63  ISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKEAL 122
           + RDF+W+G       +LV+W+    P   GGLG G +  RN+A + KWLWR+ +E  AL
Sbjct: 116 LQRDFLWSGIGEGKRDHLVRWDVVCKPKEIGGLGFGNISLRNLAFLGKWLWRYPREGSAL 175

Query: 123 WRKVVASIYETKPR--------WD--------LGN------------------------- 163
           W +++ SIY +           W+        LG+                         
Sbjct: 176 WHQIILSIYGSHSNGNGEIIWFWEDLWWGDQPLGSQYPSLFRVVLDKNIPISSVLDPTRP 235

[Close]

BLAST of Spg003010 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.6e-11
Identity = 35/97 (36.08%), Postives = 49/97 (50.52%), Query Frame = 0


Query: 42  PSSAIKAMEKISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWL 101
           P S +  ++++SR F+W     K   +LVKW     P   GGLGV A +  N ALI+K  
Sbjct: 59  PQSILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVG 118

Query: 102 WRFSQEKEALWRKVVASIYET----KPRWDLGNCTWN 135
           WR  QEK +LW  V+   Y        RW +   +W+
Sbjct: 119 WRLLQEKNSLWTLVLQKKYHVGEIRDSRWLIPKGSWS 155

[Close]

BLAST of Spg003010 vs. ExPASy TrEMBL
Match: A0A6J1E196 (uncharacterized protein LOC111025019 OS=Momordica charantia OX=3673 GN=LOC111025019 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 9.1e-26
Identity = 61/135 (45.19%), Postives = 80/135 (59.26%), Query Frame = 0


Query: 1   MAKTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMG---------------APSSA 60
           +AKTS+IGINI + E       +GC+ E +   YLG P+G               AP S 
Sbjct: 134 LAKTSLIGINIGENEATSKVDKIGCRVERMSFRYLGLPLGGNHRVIVFWRHLVEKAPVSI 193

Query: 61  IKAMEKISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFS 120
            K M KI RD  W+G       NLVKW+ +SLP+  GGLG+GA   +N AL+ KWLW F+
Sbjct: 194 TKEMNKIVRDLFWSGATSHGCKNLVKWDISSLPMKLGGLGIGAFPPKNNALLMKWLWHFT 253

[Close]

BLAST of Spg003010 vs. ExPASy TrEMBL
Match: A0A438DVJ6 (Microtubule-associated protein AIR9 OS=Vitis vinifera OX=29760 GN=AIR9_14 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.5e-23
Identity = 63/194 (32.47%), Postives = 95/194 (48.97%), Query Frame = 0


Query: 3   KTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMGAPSSA-------------IKAM 62
           K+SI GIN+D+  +   A+ L C+    P+ YLG P+G    A                +
Sbjct: 196 KSSIYGINLDQALLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVVERISMAAKI 255

Query: 63  EKISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKE 122
           E++ RDF+W+G       +LV+W+    P + GGLG+G +  RN+AL+ KWLWR+ +E  
Sbjct: 256 ERLQRDFLWSGFGEGKRDHLVRWDVVCKPKTIGGLGLGNISWRNLALLGKWLWRYPREGS 315

Query: 123 ALWRKVVASIYETKPR-WDLGNC--------------------TWNLRLCRRLFDRELPS 163
           ALW +V+ SIY +    WD                         WNL   R L D E+  
Sbjct: 316 ALWHQVILSIYGSHSNGWDANTLETGKEFGSGKICVLGPSRPFLWNLNFRRNLSDSEIED 375

[Close]

BLAST of Spg003010 vs. ExPASy TrEMBL
Match: A0A438DN42 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=Pol_32 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.6e-22
Identity = 58/170 (34.12%), Postives = 89/170 (52.35%), Query Frame = 0


Query: 3   KTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMGA-----------PSSAIKAMEK 62
           K++I GIN+++  +   A+ L C+    P+ YLG P+G            P+S    +EK
Sbjct: 219 KSNIYGINLEQNHLSRLAEMLDCKASGWPILYLGLPLGGNPKTSGFWDPIPASVAAKIEK 278

Query: 63  ISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKEAL 122
           + RDF+W+G       +LV W+    P S GGLG G +  RN+AL+ KWLWR+ +E  AL
Sbjct: 279 MQRDFLWSGVGEGKRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSAL 338

Query: 123 WRKVVASIYETKPR-WDLGN-------CTWNLRLCRRLFDRELPSWAGLV 154
           W +V+ SIY +    WD+ N       C W  +    ++ +  PS+   V
Sbjct: 339 WHQVILSIYGSHSNGWDVNNIVRWSHRCPW--KAIALVYTKSFPSFTRFV 386

[Close]

BLAST of Spg003010 vs. ExPASy TrEMBL
Match: A0A438KHD8 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_147 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 2.1e-22
Identity = 56/129 (43.41%), Postives = 77/129 (59.69%), Query Frame = 0


Query: 9   INIDKLE---------MDDWAKNLGCQCEALPMNYLGFPMGAPSSAI-KAMEKISRDFIW 68
           IN++K E         MDD A+  GC   +LP  YLG P+GAP + + + +EKI RDF+W
Sbjct: 11  INLEKSELIPVGRVESMDDLAEEFGCSLGSLPTTYLGMPLGAPFNTVRRRLEKIQRDFLW 70

Query: 69  NGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKEALWRKVVAS 127
            GGN +   +LV+WE   L  + GGLGV +L   N  L+ KW WRF+ E+EALW +V+  
Sbjct: 71  GGGNLEHKPHLVRWELVCLSKAKGGLGVKSLSLLNKTLLAKWNWRFANEREALWNQVIRG 130

[Close]

BLAST of Spg003010 vs. ExPASy TrEMBL
Match: A0A438I024 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_772 PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.3e-21
Identity = 63/212 (29.72%), Postives = 97/212 (45.75%), Query Frame = 0


Query: 3   KTSIIGINIDKLEMDDWAKNLGCQCEALPMNYLGFPMGA-----------PSSAIKAMEK 62
           K++I GIN+D+  +   A+ L C+    P+ YLG P+G            P+S    +E+
Sbjct: 56  KSNIYGINLDQAHISRLAETLECKASGWPILYLGLPLGGNPRAGGFWDPLPASVAAKIER 115

Query: 63  ISRDFIWNGGNYKLGSNLVKWEWTSLPLSHGGLGVGALRQRNIALITKWLWRFSQEKEAL 122
           + RDF+W+G       +LV+W+    P   GGLG G +  RN+A + KWLWR+ +E  AL
Sbjct: 116 LQRDFLWSGIGEGKRDHLVRWDVVCKPKEIGGLGFGNISLRNLAFLGKWLWRYPREGSAL 175

Query: 123 WRKVVASIYETKPR--------WD--------LGN------------------------- 163
           W +++ SIY +           W+        LG+                         
Sbjct: 176 WHQIILSIYGSHSNGNGEIIWFWEDLWWGDQPLGSQYPSLFRVVLDKNIPISSVLDPTRP 235

The following BLAST results are available for this feature:

BLAST of Spg003010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158566.1	1.9e-25	45.19	uncharacterized protein LOC111025019 [Momordica charantia]	[more]
RVW39501.1	5.1e-23	32.47	microtubule-associated protein AIR9 [Vitis vinifera]	[more]
RVW36866.1	3.3e-22	34.12	LINE-1 retrotransposable element ORF2 protein [Vitis vinifera]	[more]
RVX20623.1	4.3e-22	43.41	putative ribonuclease H protein [Vitis vinifera]	[more]
RVW90069.1	4.8e-21	29.72	putative ribonuclease H protein [Vitis vinifera]	[more]

BLAST of Spg003010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C2F6	1.6e-11	36.08	Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of Spg003010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E196	9.1e-26	45.19	uncharacterized protein LOC111025019 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A438DVJ6	2.5e-23	32.47	Microtubule-associated protein AIR9 OS=Vitis vinifera OX=29760 GN=AIR9_14 PE=4 S...	[more]
A0A438DN42	1.6e-22	34.12	LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=Pol_...	[more]
A0A438KHD8	2.1e-22	43.41	Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_147 P...	[more]
A0A438I024	2.3e-21	29.72	Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_772 P...	[more]

BLAST of Spg003010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003010.1	Spg003010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043170	macromolecule metabolic process
biological_process	GO:0006139	nucleobase-containing compound metabolic process
molecular_function	GO:0003824	catalytic activity