Spg003000 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003000
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	trypsin inhibitor 1-like
Location	scaffold6: 4301667 .. 4301915 (+)
RNA-Seq Expression	Spg003000
Synteny	Spg003000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAGAAACTTCGGGTGTTGGCAATGGTGGGGATGTTGCTGATGGCTGCTGTGGCGGAGTCCGCCATTGAAGCAGAGGTCGTGGGACTGAACAAAATTGATCTCCCAAGAAGAATGATGAAGCAAAATTATGAAATTAATCAGGCAAGAGTGTGCCCTCGAATCCTTGAGGAATGCACCACCGACGACGACTGCATGGATGATTGCATTTGCCTCTCCAATGGCTTCTGTGGCTTGAATTGA

mRNA sequence

ATGGAGTGGAAGAAACTTCGGGTGTTGGCAATGGTGGGGATGTTGCTGATGGCTGCTGTGGCGGAGTCCGCCATTGAAGCAGAGGTCGTGGGACTGAACAAAATTGATCTCCCAAGAAGAATGATGAAGCAAAATTATGAAATTAATCAGGCAAGAGTGTGCCCTCGAATCCTTGAGGAATGCACCACCGACGACGACTGCATGGATGATTGCATTTGCCTCTCCAATGGCTTCTGTGGCTTGAATTGA

Coding sequence (CDS)

ATGGAGTGGAAGAAACTTCGGGTGTTGGCAATGGTGGGGATGTTGCTGATGGCTGCTGTGGCGGAGTCCGCCATTGAAGCAGAGGTCGTGGGACTGAACAAAATTGATCTCCCAAGAAGAATGATGAAGCAAAATTATGAAATTAATCAGGCAAGAGTGTGCCCTCGAATCCTTGAGGAATGCACCACCGACGACGACTGCATGGATGATTGCATTTGCCTCTCCAATGGCTTCTGTGGCTTGAATTGA

Protein sequence

MEWKKLRVLAMVGMLLMAAVAESAIEAEVVGLNKIDLPRRMMKQNYEINQARVCPRILEECTTDDDCMDDCICLSNGFCGLN

Homology

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BLAST of Spg003000 vs. NCBI nr
Match: XP_031739937.1 (uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 108.2 bits (269), Expect = 3.4e-20
Identity = 57/85 (67.06%), Postives = 70/85 (82.35%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAE---SAIEAE-VVGLNKIDLPRRMMKQNYEINQARVCPR 60
          MEWKK+ +LA+VGML+MAA+ E   +AIE+E V GLNK +LPR+MM +     + R+CPR
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNE-----ETRMCPR 60

Query: 61 ILEECTTDDDCMDDCICLSNGFCGL 82
          ILEECTTDDDCM+DCICLSNGFCGL
Sbjct: 61 ILEECTTDDDCMNDCICLSNGFCGL 80

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BLAST of Spg003000 vs. NCBI nr
Match: KGN52760.1 (hypothetical protein Csa_014741 [Cucumis sativus])

HSP 1 Score: 106.3 bits (264), Expect = 1.3e-19
Identity = 56/84 (66.67%), Postives = 69/84 (82.14%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAE---SAIEAE-VVGLNKIDLPRRMMKQNYEINQARVCPR 60
          MEWKK+ +LA+VGML+MAA+ E   +AIE+E V GLNK +LPR+MM +     + R+CPR
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNE-----ETRMCPR 60

Query: 61 ILEECTTDDDCMDDCICLSNGFCG 81
          ILEECTTDDDCM+DCICLSNGFCG
Sbjct: 61 ILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Spg003000 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 73.9 bits (180), Expect = 7.0e-10
Identity = 45/87 (51.72%), Postives = 53/87 (60.92%), Query Frame = 0


Query: 1   MEWKKLRVLAMVGMLLMAAVAESA-------IEAEVVGLNKIDLPRRMMKQNYEINQARV 60
           MEWK+  ++AMVG+LLMAA AESA       I+    G+N  D PR+MM      N A  
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVN--DFPRKMM------NGALG 142

Query: 61  CPRILEECTTDDDCMDDCICLSNGFCG 81
           CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 143 CPRILMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Spg003000 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 73.9 bits (180), Expect = 7.0e-10
Identity = 45/87 (51.72%), Postives = 53/87 (60.92%), Query Frame = 0


Query: 1   MEWKKLRVLAMVGMLLMAAVAESA-------IEAEVVGLNKIDLPRRMMKQNYEINQARV 60
           MEWK+  ++AMVG+LLMAA AESA       I+    G+N  D PR+MM      N A  
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVN--DFPRKMM------NGALG 142

Query: 61  CPRILEECTTDDDCMDDCICLSNGFCG 81
           CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 143 CPRILMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Spg003000 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 73.2 bits (178), Expect = 1.2e-09
Identity = 44/87 (50.57%), Postives = 53/87 (60.92%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAESA-------IEAEVVGLNKIDLPRRMMKQNYEINQARV 60
          MEWK+  ++AMVG+LLMAA AESA       I+    G+N  D PR+MM      N  + 
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVN--DFPRKMM------NGVQG 60

Query: 61 CPRILEECTTDDDCMDDCICLSNGFCG 81
          CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 61 CPRILMECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Spg003000 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.2e-09
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 17 MAAVAESAIEAEVVGL---NKIDLPRRMMKQNYEINQARVCPRILEECTTDDDCMDDCIC 76
          MA+VAES+   EV+ L      DLPR++M   +      +CPRIL  C TDDDCM DC C
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMSGRH----GGICPRILMPCKTDDDCMLDCRC 60

Query: 77 LSNGFCG 81
          LSNG+CG
Sbjct: 61 LSNGYCG 63

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BLAST of Spg003000 vs. ExPASy Swiss-Prot
Match: Q9S8I2 (Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 7.5e-07
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 52 RVCPRILEECTTDDDCMDDCICLSNGFCG 81
          R+CPRIL EC++D DC+ +CICL NGFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLENGFCG 29

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BLAST of Spg003000 vs. ExPASy Swiss-Prot
Match: P25849 (Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.7e-06
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 0


Query: 52 RVCPRILEECTTDDDCMDDCICLSNGFCG 81
          R+CPRIL EC++D DC+ +CICL  GFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLEQGFCG 29

[Close]

BLAST of Spg003000 vs. ExPASy Swiss-Prot
Match: P83395 (Trypsin inhibitor 4 OS=Cyclanthera pedata OX=198836 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.4e-05
Identity = 19/29 (65.52%), Postives = 21/29 (72.41%), Query Frame = 0


Query: 52 RVCPRILEECTTDDDCMDDCICLSNGFCG 81
          R+CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 1  RICPRILMECKADSDCLAQCICQENGFCG 29

[Close]

BLAST of Spg003000 vs. ExPASy Swiss-Prot
Match: P35628 (Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.4e-05
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 0


Query: 53 VCPRILEECTTDDDCMDDCICLSNGFCG 81
          +CPRIL  C++D DC+ +CICL NGFCG
Sbjct: 1  ICPRILMPCSSDSDCLAECICLENGFCG 28

[Close]

BLAST of Spg003000 vs. ExPASy TrEMBL
Match: A0A0A0KT01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.2e-20
Identity = 56/84 (66.67%), Postives = 69/84 (82.14%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAE---SAIEAE-VVGLNKIDLPRRMMKQNYEINQARVCPR 60
          MEWKK+ +LA+VGML+MAA+ E   +AIE+E V GLNK +LPR+MM +     + R+CPR
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNE-----ETRMCPR 60

Query: 61 ILEECTTDDDCMDDCICLSNGFCG 81
          ILEECTTDDDCM+DCICLSNGFCG
Sbjct: 61 ILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Spg003000 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.8e-10
Identity = 44/87 (50.57%), Postives = 53/87 (60.92%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAESA-------IEAEVVGLNKIDLPRRMMKQNYEINQARV 60
          MEWK+  ++AMVG+LLMAA AESA       I+    G+N  D PR+MM      N  + 
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVN--DFPRKMM------NGVQG 60

Query: 61 CPRILEECTTDDDCMDDCICLSNGFCG 81
          CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 61 CPRILMECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Spg003000 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.3e-06
Identity = 35/83 (42.17%), Postives = 47/83 (56.63%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAES---AIEAEVVGLNKIDLPRRMMKQNYEINQARVCPRI 60
          MEWKK+ ++AM+GMLLMA   ES    ++ E++ L  +        QN   + A  CPRI
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQL--VSDGVNEYSQNIGKDTAPGCPRI 60

Query: 61 LEECTTDDDCMDDCICLSNGFCG 81
          L +C TD DC   C C   G+CG
Sbjct: 61 LMKCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Spg003000 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.7e-06
Identity = 34/83 (40.96%), Postives = 47/83 (56.63%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAES---AIEAEVVGLNKIDLPRRMMKQNYEINQARVCPRI 60
          MEWKK+ ++AM+GMLLMA   ES    ++ E++ L        +     E +    CPRI
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQL--------VSDGVNEYSGHPGCPRI 60

Query: 61 LEECTTDDDCMDDCICLSNGFCG 81
          L +C TD DC+  C C  NG+CG
Sbjct: 61 LMKCKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of Spg003000 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.3e-05
Identity = 34/92 (36.96%), Postives = 51/92 (55.43%), Query Frame = 0


Query: 1  MEWKKLRVLAMVGMLLMAAVAESAIEAEVVGLNKIDL-----------PRR-MMKQNYEI 60
          M+ KK+ V+A+VGM++M A   S+    V   +  +L           PR+ M+K     
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60

Query: 61 NQARVCPRILEECTTDDDCMDDCICLSNGFCG 81
           + R+CP+IL +C  D DC+ DC+CL  GFCG
Sbjct: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

The following BLAST results are available for this feature:

BLAST of Spg003000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739937.1	3.4e-20	67.06	uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
KGN52760.1	1.3e-19	66.67	hypothetical protein Csa_014741 [Cucumis sativus]	[more]
KAG6576915.1	7.0e-10	51.72	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	7.0e-10	51.72	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022984819.1	1.2e-09	50.57	trypsin inhibitor 1-like [Cucurbita maxima]	[more]

BLAST of Spg003000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34950	1.2e-09	52.24	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q9S8I2	7.5e-07	68.97	Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P25849	3.7e-06	65.52	Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P83395	1.4e-05	65.52	Trypsin inhibitor 4 OS=Cyclanthera pedata OX=198836 PE=1 SV=1	[more]
P35628	1.4e-05	64.29	Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of Spg003000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT01	6.2e-20	66.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
A0A6J1JBL8	5.8e-10	50.57	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A0A0KY34	2.3e-06	42.17	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A5D3E0W0	8.7e-06	40.96	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]
A0A5D3E148	3.3e-05	36.96	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]

BLAST of Spg003000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 52..80 e-value: 5.5E-12 score: 55.8
None	No IPR available	GENE3D	4.10.75.20		coord: 35..80 e-value: 6.3E-15 score: 56.7
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 52..80 e-value: 3.7E-14 score: 52.4
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 54..73
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 50..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003000.1	Spg003000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity