Spg002991 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002991
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	autophagy-related protein 3
Location	scaffold6: 2714674 .. 2718294 (-)
RNA-Seq Expression	Spg002991
Synteny	Spg002991

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCATTGGTCCAGAGGCATGGCGGAGAAGGTCAAAAGTTCTTTCAGGACCTTTCCGTTCAAAGAAAGGCTCGTCGTCCTCACCGTCCCTGAGTTCATTGCCGCCGGCGATTGCCTCGTCGCACAATGTCCTTCTTGGTCTTGGTATTCTGCTTTTCTTTTTTCCTACAACTTCTTATGAACACCGTGATTTTGTGGATGCTACTGTAGTTTAGTTTTCCTAGAAAGACTTTCTGCTAAAGAATTACTGTCGTTCATTCGATTTTTAGGGAATCTGGGGATTTCAGTAAACGGAGGTCGTATTTACCACCACACAAACAGTTTTTGGTTTTAAGAAATGGTCAGTTGTGTTATCTTTTGCTGTATTATCCATTTCGGTCATTATGTTCTTGACATTTCTGGACTTTGGATGCCTTATAATCATCAAGAAAGCTGAGAATTCTCACTCTTTGGGAAGTTTTCCCCTCAAAGTTGGGTGATGTTTCAGGTTTAATGGTTTAGAATCGTACACGGCGTATTAGAGCTATACTTGTGATAAGTGATTTCACTTGAGAACACCGTAATGGTGACAGAAAGAAGTTTTCTTTTGTATAATATATTCTTTAATTCTTCAAGGATCAATAGGAGCCCAAATCGTGGTAATTAAAATACTTCCCTGTAAGTTCCATTTTACTCTAGCCAAGATCTAGTTCTTTGTTTCTAGTTTGTTCTGTTTCATTCTTATTAGCAAGGACCTTTTCTTTTCCTAATGCGACAGAAATAGAGATGGAGAGCTAGAAGATAATTATAATGATACGTGTCTTAGTTACTGAAAAGTTTTCATGCAAGACAGGATCGGGAAAAATGTCCAAATTCATCACAACTTGAGTTCTTAGCTGGACATAAAGTTTGATGTTGTTCTTTTTAGAACGAAATAATATTAGATAGAAAGAACCCCTTTTATATGAGAGCGGCTTCTAATGCTGAGCTTATTTTTCAGTTCCTTGTCCACGAAGGGGTTTCTCTTATGAAGAAGAATATGTGATTGTTGGTGGTGGTGACGAAGATGATGATCAGCAGCTCATTCGTAGGAATCCAAGTGGTCTGCTCCAGAAATTTAACATATTTCATATCTATGCTGGTTTCTCCTGATTGTTCTGTCTTAAATATGTTCTAAATCAGTGTTCTAATTTTCTTTTCTCATTAAAAGAGTCTATGACTGAGGATAACTTGCTTTCAACCGAAGAATTGTCTGGACTAAACATGAACTTGAAGACTCCCATTCCGATAATTCATTCATACTTTCATGTTAATAACAAAGAAGATATACCAGATATGGCAAAGCCAATTAGAATCTATCCTGTAAGTTTGTAATTGCAATGCAACCATAGATATTCAACTCAGGATGTTTATTGAGTATTTTTAGATTGTCATCAAAATATGTATACACAGGCAACTCAGCAATCTACTCACCTTGTAGTCCATGAACCTGAAGACAACATATTCCCAAGAACTTATGACATTAGCATCACGTGAGATAATTTTTCAATGTCTTTGGTCCTTTTTCGATAATTAATTGTGTACTTTATTGGAAAATTATACTTTTGTAGACTAATCTGCTTTATATGAGAACCTCTCGTTATATTCACATTTTAAAAAGCTTTATTATGGTTTGTTAATCTCATCATCTCCTGTTGTTGCAGTTATGACAAAGATCATCAGACCCCCCATATTTGGTTTACTGGATATCACGAGGTTTGCACTTAAATCTGTCAGGTTTTGGCTTTGTATTACTGAAATATAATGGTTGTGATAGGGAGGTGGCTTGTACTTGAGTCTATTATGAACACTTAGGGTTATAAATTTCTAATCCTAAGACGTTGCAGAGTATGAAGATGCATTTTGCCATCCTCACCATAAATTATTTATGATTTAGCTGATGACTTGAATTTTATTTCATCTTTAGAAGTTGTGAATGTGGATAACATGTAAACAATGAATTATAATAGGACTCTACCCTTTTAGCTTGCTTTACTCTTGTAGAAAAGAGTATGAAATGGGAATGGGCAACCACTGAACAGTTTTGTGGCTTAAGCCTTCTTTCTAGATGGGGTTTTCTTATGACCTCTGGCTCTCCAGGTTTCTGAAATCACTTTGAAAAGTGAACCATAAATGCATAACGTTCTCTTATCATTGGTGGAAGCGGTCCTCTAAAAAAGAGTTTGCAACTCGGATAGCGATATACATTCCATGCCATTCCTGATTCTTGCTCCTCGACGATAAATATAAAAACAGTTTCGGAAAGAAAATAATTTTGTAGGTACCTTTAAAACATTCACTCATGTTTTCTACATCAAACTTCATCAGGAAGATGAAATCGTAAATATTTTTGCATAATTGATGAAATGTTTGTTCGTATTATCAAATTAGTGAGTTAATAGCAAGCTACATTTCTACCTGTTACCATACATTTATTAACCTAAGTACACCATATCTTCCTCGTCTTTTCATTATTTTCTTCTGTTGTTGTATTTTGGCAGAACGGGATGCACTTGCAACCAGAACTAATTTTTGAAGATGTTAAGCAGAATCACGTAGGCAAAACAGTAAGACCATATCTACTGGCCATAGATTTTCTTTCAAACATATGTTGGTAATCCAGAAAGATAACAAAGAACAGAGTTTTACAAAAAATGGCCCCGATTAGCATAAGAGGCAAAGTTATGTGATTAAAAAGGACAAGATTAATCTTACACCAAAAAGAGCCAACCACCTGGCTAAATTTCTCACCTCGTCTCATTCCTTCTAATCCTAGAGTTTTCCTTTTCCACTGAAAGCTCTCAGTAGTGCCTTGACAGCAATAGACTAAGGCCTATCTGGAATGACTTTTCAAGTGTTAAAACTTGGTTTTTAAGTACATAAAACGTCATTCCAAACAGATTCCGAATGATCTTGGGTGTTGATAGGTATAGATACTATCCTAGCATATATTAGATATACTGGAAGCAATATTGTTTCACTCCCTATTACTCTTTTCTTGATTTTTTTTTTTTTCTTTTTATCTCTCTTTTGTGAGGAAGACTTGTAAATAGCAATTCTTGAGAAGATAAGAATCATCTGATTACTATGTCCAAAGGAGATCACGAACGAAAAAAAGTACATACGAGCAGAGAAAAAACCTCTTCAAATGGCATTACTCTAGAAATAAAAACAATAAATCAAATTAAGATATCTACTTTTTCCATAAAAGAACTCTATTGTAATCTCGATATGCAGGTAAGTATTGAAGACCATCCTTACTTGTTTGGGAAACATGCATCTGTACATCCAAGAGTTCATGGAGTTGCAGTGAAGAAAATGGTTGATGACTGGACGTCATGTGGGATTGTGCCACAAGCAGACCGGTAATTTCACCTTCTCTTTACCTTGCTCAATCATATTTTGATTTTTTTTCTGATTCCTTGGATTTAAATTTAAGCAAACCCATCAGCATAAAAAAAGAGAGATACTTGTAAGAAAAGTGAAGGTGTTAGAAGGATCTGTGAACTTTCTTTTACTTTGCAGGTACCTTTTTCTATTTTTGGAATTTGTTGCTTCAGTATTTCCAACTATTAAACTTGATTACAACATGAGCTTTTGA

mRNA sequence

ATGGCTCATTGGTCCAGAGGCATGGCGGAGAAGGTCAAAAGTTCTTTCAGGACCTTTCCGTTCAAAGAAAGGCTCGTCGTCCTCACCGTCCCTGAGTTCATTGCCGCCGGCGATTGCCTCGTCGCACAATGTCCTTCTTGGTCTTGGGAATCTGGGGATTTCAGTAAACGGAGGTCGTATTTACCACCACACAAACAGTTTTTGGTTTTAAGAAATGGTCAGTTGTGTTATCTTTTGCTGTATTATCCATTTCGGGGTTTCTCTTATGAAGAAGAATATGTGATTGTTGGTGGTGGTGACGAAGATGATGATCAGCAGCTCATTCGTAGGAATCCAAAGTCTATGACTGAGGATAACTTGCTTTCAACCGAAGAATTGTCTGGACTAAACATGAACTTGAAGACTCCCATTCCGATAATTCATTCATACTTTCATGTTAATAACAAAGAAGATATACCAGATATGGCAAAGCCAATTAGAATCTATCCTGCAACTCAGCAATCTACTCACCTTGTAGTCCATGAACCTGAAGACAACATATTCCCAAGAACTTATGACATTAGCATCACTTATGACAAAGATCATCAGACCCCCCATATTTGGTTTACTGGATATCACGAGAACGGGATGCACTTGCAACCAGAACTAATTTTTGAAGATGTTAAGCAGAATCACGTAGGCAAAACAGTAAGTATTGAAGACCATCCTTACTTGTTTGGGAAACATGCATCTGTACATCCAAGAGTTCATGGAGTTGCAGTGAAGAAAATGGTTGATGACTGGACGTCATGTGGGATTGTGCCACAAGCAGACCGGTACCTTTTTCTATTTTTGGAATTTGTTGCTTCAGTATTTCCAACTATTAAACTTGATTACAACATGAGCTTTTGA

Coding sequence (CDS)

ATGGCTCATTGGTCCAGAGGCATGGCGGAGAAGGTCAAAAGTTCTTTCAGGACCTTTCCGTTCAAAGAAAGGCTCGTCGTCCTCACCGTCCCTGAGTTCATTGCCGCCGGCGATTGCCTCGTCGCACAATGTCCTTCTTGGTCTTGGGAATCTGGGGATTTCAGTAAACGGAGGTCGTATTTACCACCACACAAACAGTTTTTGGTTTTAAGAAATGGTCAGTTGTGTTATCTTTTGCTGTATTATCCATTTCGGGGTTTCTCTTATGAAGAAGAATATGTGATTGTTGGTGGTGGTGACGAAGATGATGATCAGCAGCTCATTCGTAGGAATCCAAAGTCTATGACTGAGGATAACTTGCTTTCAACCGAAGAATTGTCTGGACTAAACATGAACTTGAAGACTCCCATTCCGATAATTCATTCATACTTTCATGTTAATAACAAAGAAGATATACCAGATATGGCAAAGCCAATTAGAATCTATCCTGCAACTCAGCAATCTACTCACCTTGTAGTCCATGAACCTGAAGACAACATATTCCCAAGAACTTATGACATTAGCATCACTTATGACAAAGATCATCAGACCCCCCATATTTGGTTTACTGGATATCACGAGAACGGGATGCACTTGCAACCAGAACTAATTTTTGAAGATGTTAAGCAGAATCACGTAGGCAAAACAGTAAGTATTGAAGACCATCCTTACTTGTTTGGGAAACATGCATCTGTACATCCAAGAGTTCATGGAGTTGCAGTGAAGAAAATGGTTGATGACTGGACGTCATGTGGGATTGTGCCACAAGCAGACCGGTACCTTTTTCTATTTTTGGAATTTGTTGCTTCAGTATTTCCAACTATTAAACTTGATTACAACATGAGCTTTTGA

Protein sequence

MAHWSRGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAKPIRIYPATQQSTHLVVHEPEDNIFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDHPYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYNMSF

Homology

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BLAST of Spg002991 vs. NCBI nr
Match: XP_022141116.1 (autophagy-related protein 3 [Momordica charantia])

HSP 1 Score: 306.2 bits (783), Expect = 3.0e-79
Identity = 165/301 (54.82%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 1   MAHW---SRGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKR 60
           MA+W    +GM EK+KSS +    KE LVVLTVPEFIAAGD L+A+CP WSW+SG+ SK 
Sbjct: 1   MANWLKGFKGMVEKIKSSLKISQSKESLVVLTVPEFIAAGDHLIAECPDWSWDSGELSKV 60

Query: 61  RSYLPPHKQFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTE 120
           +SYLP HKQFLV R+            R  SYE+EY+IVG   EDD + L + NP     
Sbjct: 61  KSYLPSHKQFLVTRSVSCLR-------RASSYEDEYMIVGDDGEDDSRPLFQENP----- 120

Query: 121 DNLLSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAKPIRIYPATQQSTHLVVHEPE 180
                TE +  +N++      +  SYF V NKE  PDM K I IY  TQ STH +VHEPE
Sbjct: 121 -----TESVEEVNLHSL----LTDSYFGVGNKEYTPDMEKCIGIYSGTQDSTHPMVHEPE 180

Query: 181 DN--IFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDH 240
           DN    PRTYDISITYD+D+QTP  W TGYH+  M L+PELI EDV+++  GKTV+IEDH
Sbjct: 181 DNDVASPRTYDISITYDRDYQTPRFWLTGYHKYRMCLEPELILEDVRRDLAGKTVTIEDH 240

Query: 241 PYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYNMS 297
           P+L GKHASVHPR  G AVKKMVDD  S GI  +  +YL LFL+ VASVFP+I     + 
Sbjct: 241 PHLLGKHASVHPRYQGAAVKKMVDDCLSRGIELEVRKYLLLFLKSVASVFPSIDFGDTID 280

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BLAST of Spg002991 vs. NCBI nr
Match: XP_004146945.2 (autophagy-related protein 3 [Cucumis sativus] >KAE8649136.1 hypothetical protein Csa_014617 [Cucumis sativus])

HSP 1 Score: 298.9 bits (764), Expect = 4.8e-77
Identity = 163/277 (58.84%), Postives = 191/277 (68.95%), Query Frame = 0


Query: 22  KERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQFLVLRNGQLCYLLLY 81
           +E+ +V T  +FI AG+ LV    SWSWESGD SK++S LP  KQFL+LRN         
Sbjct: 15  REKTIVFTEADFITAGNFLVGNSDSWSWESGDLSKQKSNLPSDKQFLILRNAPCLR---- 74

Query: 82  YPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEELSGLNMNLKTPIPIIH 141
              R FSY+EEYVIVG   ED+ + L     +S  EDNLLST+EL         PI II 
Sbjct: 75  ---RAFSYKEEYVIVGSDGEDNARLL----SESKDEDNLLSTKELP--KPGTMKPITIIP 134

Query: 142 SYFHVNNKED--IPDMAKPIRIYPATQQSTHLVVHEPEDNIFPRTYDISITYDKDHQTPH 201
           SYF V+ KED  +P M K I++  ATQ+STH VVH+ ED I  RTYDISITYD DHQT  
Sbjct: 135 SYFAVDKKEDLPLPYMEKSIKLCSATQESTHRVVHDSEDIICLRTYDISITYDNDHQTFR 194

Query: 202 IWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDHPYLFGKHASVHPRVHGVAVKKMVD 261
            W TGYHE+G  LQPELIFEDV+Q+HVGKTV+IEDHPYL GKHASVHP++HG AVKKMV 
Sbjct: 195 FWLTGYHEDGRPLQPELIFEDVRQSHVGKTVNIEDHPYLPGKHASVHPKIHGAAVKKMVK 254

Query: 262 DWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYNMSF 297
              S G VP+  + L LFLEFVASVFPTIK D N+ F
Sbjct: 255 CRMSQGNVPEVSKCLSLFLEFVASVFPTIKYDCNIGF 278

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BLAST of Spg002991 vs. NCBI nr
Match: XP_008453898.1 (PREDICTED: autophagy-related protein 3 isoform X1 [Cucumis melo])

HSP 1 Score: 293.1 bits (749), Expect = 2.7e-75
Identity = 160/282 (56.74%), Postives = 190/282 (67.38%), Query Frame = 0


Query: 17  RTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQFLVLRNGQLC 76
           R    +ER ++ T  +FI AG+ LV    SWSWESGD SK++SYLP  KQFL+LRN    
Sbjct: 10  RPSQIRERTMLFTETDFITAGNFLVGNFDSWSWESGDLSKQKSYLPSDKQFLILRNAPC- 69

Query: 77  YLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEELSGLNMNLKTP 136
                 P R FSYEE YVIVG   ED+D+ L     +S   DNLLST+EL         P
Sbjct: 70  ------PRRAFSYEEGYVIVGSDGEDNDRLL----SESKDVDNLLSTKELP--KPGTMKP 129

Query: 137 IPIIHSYFHVNNKED--IPDMAKPIRIYPATQQSTHLVVHEPEDNIFPRTYDISITYDKD 196
           I II SYF V+ KED  +P + K I++  ATQ+STH VVH+ ED I  RTYDISITYD D
Sbjct: 130 ITIIPSYFAVDKKEDLSLPYVEKSIKLCSATQESTHRVVHDSEDIICLRTYDISITYDND 189

Query: 197 HQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDHPYLFGKHASVHPRVHGVAV 256
           H+T H W TGYHE+G  LQPELI EDV+++HVGKTV+IEDHPYL GKHASVHP++HG AV
Sbjct: 190 HRTFHFWLTGYHEDGRPLQPELILEDVRKSHVGKTVNIEDHPYLPGKHASVHPKIHGAAV 249

Query: 257 KKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYNMSF 297
           KKMV    S G V +  + L LFLEF ASVFPTI  D N+ F
Sbjct: 250 KKMVKSRMSQGNVSEVSKCLSLFLEFFASVFPTIIYDCNIGF 278

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BLAST of Spg002991 vs. NCBI nr
Match: XP_038879221.1 (LOW QUALITY PROTEIN: autophagy-related protein 3 [Benincasa hispida])

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-71
Identity = 155/276 (56.16%), Postives = 186/276 (67.39%), Query Frame = 0


Query: 21  FKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQFLVLRNGQLCYLLL 80
           FKER +V TV +FI AGD LVA+C SWSWES D  K++ YLP +KQF +LRN        
Sbjct: 12  FKERNIVFTVSDFITAGDFLVAKC-SWSWESDDLIKQKXYLPSNKQFFILRNVPCLR--- 71

Query: 81  YYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEELSGLNMNLKTPIPII 140
               R  SYEEEYVI G  +  D              DNLLST++L  L ++   PI +I
Sbjct: 72  ----RALSYEEEYVIAGSDESKDG-------------DNLLSTKKL--LELDTNNPITVI 131

Query: 141 HSYFHVNNKEDIPDMAKPIRIYPATQQSTHLVVHEPEDNIFPRTYDISITYDKDHQTPHI 200
           HSYF V+     P M K I+ +PA Q+STHLVVH+ ED+I PRTYDISITYD D QT H 
Sbjct: 132 HSYFGVDKN---PYMEKSIKFHPAAQESTHLVVHDSEDSICPRTYDISITYDNDLQTFH- 191

Query: 201 WFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDHPYLFGKHASVHPRVHGVAVKKMVDD 260
           + TGY ++GM LQPELI EDV++NH GK V+IEDHPYL GKHASVHP++ G AVKKMV+ 
Sbjct: 192 FXTGYQKDGMPLQPELILEDVRKNHAGKAVNIEDHPYLPGKHASVHPKILGAAVKKMVEY 251

Query: 261 WTSCGIVPQADRYLFLFLEFVASVFPTIKLDYNMSF 297
             S   VP+  R+L LFLEFVASVFPTIK D  M+F
Sbjct: 252 QMSHWNVPEVSRFLSLFLEFVASVFPTIKYDCKMNF 260

[Close]

BLAST of Spg002991 vs. NCBI nr
Match: XP_012090471.1 (autophagy-related protein 3 isoform X2 [Jatropha curcas])

HSP 1 Score: 277.3 bits (708), Expect = 1.5e-70
Identity = 155/304 (50.99%), Postives = 195/304 (64.14%), Query Frame = 0


Query: 6   RGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHK 65
           +G  E++        FKE+  VL+V EFI AGD LV++CP+WSWESG+ +KR+SYLPP K
Sbjct: 12  KGTVERITGPRTVSAFKEK-GVLSVSEFILAGDNLVSKCPTWSWESGEPNKRKSYLPPEK 71

Query: 66  QFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGG----GDEDDDQQLIRR-NPKSMTEDNL 125
           QFL+ RN            R  S EEEY   GG     +ED+D  L     PK   +DN+
Sbjct: 72  QFLITRNVPCLR-------RAASVEEEYEAAGGEILLDNEDNDGWLATHGKPKGDEDDNV 131

Query: 126 LSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMA------KPIRIYPATQQSTHLVVH 185
            S E    L ++ K PI  I +YF    +EDIPDMA        I   PAT QST+LV H
Sbjct: 132 PSME---ALEISKKNPIQPIPTYFGGEEEEDIPDMADYEDPDNLIETDPATLQSTYLVAH 191

Query: 186 EPEDN--IFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSI 245
           EP+D+  +  RTYD+SITYDK +QTP +W TGY E+ M LQPEL+ EDV Q+H  KTV+I
Sbjct: 192 EPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKTVTI 251

Query: 246 EDHPYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDY 297
           EDHP+L GKHASVHP  HG  +KK++D   S G+ P+ D+YLFLFL+FVASV PTI+ DY
Sbjct: 252 EDHPHLPGKHASVHPCQHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFVASVIPTIEYDY 304

[Close]

BLAST of Spg002991 vs. ExPASy Swiss-Prot
Match: Q0WWQ1 (Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2)

HSP 1 Score: 265.0 bits (676), Expect = 1.0e-69
Identity = 149/306 (48.69%), Postives = 191/306 (62.42%), Query Frame = 0


Query: 6   RGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHK 65
           +G  E++        FKE+  VL+V EF+ AGD LV++CP+WSWESGD SKR+ YLP  K
Sbjct: 12  KGTVERITGPRTISAFKEK-GVLSVSEFVLAGDNLVSKCPTWSWESGDASKRKPYLPSDK 71

Query: 66  QFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGG----GDEDDDQQLI---RRNPKSMTED 125
           QFL+ RN            R  S  E+Y   GG     DED+D  L    +   K   ED
Sbjct: 72  QFLITRNVPCLR-------RAASVAEDYEAAGGEVLVDDEDNDGWLATHGKPKDKGKEED 131

Query: 126 NLLSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAK------PIRIYPATQQSTHLV 185
           NL S   +  L++N K  I  I +YF     +DIPDM +       +   PAT QST+LV
Sbjct: 132 NLPS---MDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDEADNVVENDPATLQSTYLV 191

Query: 186 VHEPEDN--IFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTV 245
            HEP+D+  +  RTYD+SITYDK +QTP +W TGY E+ M LQPEL+ EDV Q+H  KTV
Sbjct: 192 AHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLLQPELVMEDVSQDHARKTV 251

Query: 246 SIEDHPYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKL 297
           +IEDHP+L GKHASVHP  HG  +KK++D   S G+ P+ D+YLFLFL+F+ASV PTI+ 
Sbjct: 252 TIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIEY 306

[Close]

BLAST of Spg002991 vs. ExPASy Swiss-Prot
Match: Q550A8 (Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV=3)

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-40
Identity = 112/326 (34.36%), Postives = 159/326 (48.77%), Query Frame = 0


Query: 10  EKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQFLV 69
           EKV  +  T  F E   VLT  EF+ AGD L  +C +W+WESGD S+  SYLP  KQFL+
Sbjct: 18  EKVTPTLSTSKFLEE-GVLTPEEFVQAGDLLTDKCQTWTWESGDPSRNVSYLPKEKQFLL 77

Query: 70  LRNGQLCY---LLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEEL 129
            RN   CY     L    +    +E  +     ++D +   +   P    +D      ++
Sbjct: 78  TRNVP-CYNRVRTLENESKASKADEIQI-----EDDGEDSWVAPQPVGNQDDIEDEKVDI 137

Query: 130 SGLNMNLKTPIPIIHSYFHVNNKE-------------DIPDM------------------ 189
           S L ++ K P     +     N               DIPD+                  
Sbjct: 138 SSLKIDDKKPNTTTTTTAKPTNNNNNNNDDEDEDEDGDIPDLDDFQDDNIIEEEDPAVLS 197

Query: 190 --AKPIRIYPATQQSTHLVVHEPEDN---IFPRTYDISITYDKDHQTPHIWFTGYHENGM 249
              K      A   + +   ++ EDN   +  RTYDISITYDK +QTP +W  GY EN  
Sbjct: 198 KNNKTTTTTTANNNNNNNSENKVEDNDNILRTRTYDISITYDKYYQTPRVWLFGYDENRK 257

Query: 250 HLQPELIFEDVKQNHVGKTVSIEDHPYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQA 297
            L+PE IFED+ ++H  KTV+I+ HP+L    A +HP  H   +KK+VD  +  G  P+ 
Sbjct: 258 PLKPEEIFEDISEDHAHKTVTIDSHPHLGISFAYIHPCRHAAVMKKLVDRQSENGKEPRV 317

[Close]

BLAST of Spg002991 vs. ExPASy Swiss-Prot
Match: Q6PFS7 (Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.2e-32
Identity = 102/308 (33.12%), Postives = 151/308 (49.03%), Query Frame = 0


Query: 7   GMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQ 66
           G+AE +    +   FKE   V+T  EF+AAGD LV  CP+W W SG+ +K + YLP  KQ
Sbjct: 14  GVAEFLTPVLKESKFKE-TGVITPEEFVAAGDHLVHHCPTWKWASGEEAKVKPYLPNDKQ 73

Query: 67  FLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMT----EDNLLS 126
           FL+ RN     +  Y   +   Y +E   +   D+ D   +   +   +T        +S
Sbjct: 74  FLLTRN-----VPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTFHNSGVTGVTEAVREIS 133

Query: 127 TEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAK-----PIRIYPATQQSTHLVVHEP- 186
            +    +NMN+KT           + + +  DM +      +    AT  ++ +      
Sbjct: 134 LDNKDNMNMNVKTGACGNSGDDDDDEEGEAADMEEYEESGLLETDDATLDTSKMADLSKT 193

Query: 187 ------EDNIF-PRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKT 246
                 ED I   RTYD+ ITYDK +QTP +W  GY E+   L  + ++ED+ Q+HV KT
Sbjct: 194 KAEAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVDQMYEDISQDHVKKT 253

Query: 247 VSIEDHPYL-FGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTI 297
           V+IE+HP L      SVHP  H   +KK+++     G       YL +FL+FV +V PTI
Sbjct: 254 VTIENHPNLPPPAMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTI 313

[Close]

BLAST of Spg002991 vs. ExPASy Swiss-Prot
Match: Q5I0S6 (Ubiquitin-like-conjugating enzyme ATG3 OS=Xenopus tropicalis OX=8364 GN=atg3 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.2e-31
Identity = 102/303 (33.66%), Postives = 146/303 (48.18%), Query Frame = 0


Query: 8   MAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQF 67
           +AE +    +   FKE   V+T  EF+AAGD LV  CP+W W +G+ SK + YLP  KQF
Sbjct: 15  VAEYLTPVLKESKFKE-TGVITPEEFLAAGDHLVHHCPTWQWSAGEESKIKPYLPNDKQF 74

Query: 68  LVLRNGQLCYLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMT------EDNLL 127
           L+ +N   CY         +S E+E +I    ++D D   +     S+T      ++  L
Sbjct: 75  LMTKNVP-CYKRCKQ--MEYSDEQEAII---EEDDGDGGWVDTFHHSLTGVTEAVKEITL 134

Query: 128 STEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAKPIRIYPATQQSTHLVVHEP----- 187
            T++      N+              + ED  +             S      +P     
Sbjct: 135 ETQDCGKTTSNIAVDDDDDDDEGEAADMEDYEESGLLDNDDATVDTSKIKEACKPKADLG 194

Query: 188 -EDNIF-PRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIED 247
            ED I   RTYD+ ITYDK +QTP +W  GY E    L  E ++ED+ Q+HV KTV+IE+
Sbjct: 195 GEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRRPLTVENMYEDISQDHVKKTVTIEN 254

Query: 248 HPYL-FGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYN 297
           HP+L      SVHP  H   +KK+++     G       YL +FL+FV +V PTI+ DY 
Sbjct: 255 HPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYT 310

[Close]

BLAST of Spg002991 vs. ExPASy Swiss-Prot
Match: Q9CPX6 (Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.4e-31
Identity = 101/308 (32.79%), Postives = 149/308 (48.38%), Query Frame = 0


Query: 8   MAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQF 67
           +AE +    +   FKE   V+T  EF+AAGD LV  CP+W W +G+  K ++YLP  KQF
Sbjct: 15  VAEYLTPVLKESKFKE-TGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTDKQF 74

Query: 68  LVLRNGQLCYLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEELS 127
           LV +N     +  Y   +   Y +E   +   D+ D   +   +   +T      TE + 
Sbjct: 75  LVTKN-----VPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITG----ITEAVK 134

Query: 128 GLNMNLKTPIPI--IHSYFHVNNKEDIPDMAKP--------IRIYPATQQSTHLVV---- 187
            + +  K  I +    +     ++ED  + A          +    AT  +  +V     
Sbjct: 135 EITLESKDSIKLQDCSALCDEEDEEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKA 194

Query: 188 ---HEPEDNIF-PRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKT 247
                 ED I   RTYD+ ITYDK +QTP +W  GY E    L  E ++ED+ Q+HV KT
Sbjct: 195 KADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKT 254

Query: 248 VSIEDHPYL-FGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTI 297
           V+IE+HP+L      SVHP  H   +KK+++     G       YL +FL+FV +V PTI
Sbjct: 255 VTIENHPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTI 312

[Close]

BLAST of Spg002991 vs. ExPASy TrEMBL
Match: A0A6J1CI24 (autophagy-related protein 3 OS=Momordica charantia OX=3673 GN=LOC111011588 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.5e-79
Identity = 165/301 (54.82%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 1   MAHW---SRGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKR 60
           MA+W    +GM EK+KSS +    KE LVVLTVPEFIAAGD L+A+CP WSW+SG+ SK 
Sbjct: 1   MANWLKGFKGMVEKIKSSLKISQSKESLVVLTVPEFIAAGDHLIAECPDWSWDSGELSKV 60

Query: 61  RSYLPPHKQFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTE 120
           +SYLP HKQFLV R+            R  SYE+EY+IVG   EDD + L + NP     
Sbjct: 61  KSYLPSHKQFLVTRSVSCLR-------RASSYEDEYMIVGDDGEDDSRPLFQENP----- 120

Query: 121 DNLLSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAKPIRIYPATQQSTHLVVHEPE 180
                TE +  +N++      +  SYF V NKE  PDM K I IY  TQ STH +VHEPE
Sbjct: 121 -----TESVEEVNLHSL----LTDSYFGVGNKEYTPDMEKCIGIYSGTQDSTHPMVHEPE 180

Query: 181 DN--IFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDH 240
           DN    PRTYDISITYD+D+QTP  W TGYH+  M L+PELI EDV+++  GKTV+IEDH
Sbjct: 181 DNDVASPRTYDISITYDRDYQTPRFWLTGYHKYRMCLEPELILEDVRRDLAGKTVTIEDH 240

Query: 241 PYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYNMS 297
           P+L GKHASVHPR  G AVKKMVDD  S GI  +  +YL LFL+ VASVFP+I     + 
Sbjct: 241 PHLLGKHASVHPRYQGAAVKKMVDDCLSRGIELEVRKYLLLFLKSVASVFPSIDFGDTID 280

[Close]

BLAST of Spg002991 vs. ExPASy TrEMBL
Match: A0A1S3BWT8 (autophagy-related protein 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494478 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 1.3e-75
Identity = 160/282 (56.74%), Postives = 190/282 (67.38%), Query Frame = 0


Query: 17  RTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQFLVLRNGQLC 76
           R    +ER ++ T  +FI AG+ LV    SWSWESGD SK++SYLP  KQFL+LRN    
Sbjct: 10  RPSQIRERTMLFTETDFITAGNFLVGNFDSWSWESGDLSKQKSYLPSDKQFLILRNAPC- 69

Query: 77  YLLLYYPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEELSGLNMNLKTP 136
                 P R FSYEE YVIVG   ED+D+ L     +S   DNLLST+EL         P
Sbjct: 70  ------PRRAFSYEEGYVIVGSDGEDNDRLL----SESKDVDNLLSTKELP--KPGTMKP 129

Query: 137 IPIIHSYFHVNNKED--IPDMAKPIRIYPATQQSTHLVVHEPEDNIFPRTYDISITYDKD 196
           I II SYF V+ KED  +P + K I++  ATQ+STH VVH+ ED I  RTYDISITYD D
Sbjct: 130 ITIIPSYFAVDKKEDLSLPYVEKSIKLCSATQESTHRVVHDSEDIICLRTYDISITYDND 189

Query: 197 HQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDHPYLFGKHASVHPRVHGVAV 256
           H+T H W TGYHE+G  LQPELI EDV+++HVGKTV+IEDHPYL GKHASVHP++HG AV
Sbjct: 190 HRTFHFWLTGYHEDGRPLQPELILEDVRKSHVGKTVNIEDHPYLPGKHASVHPKIHGAAV 249

Query: 257 KKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYNMSF 297
           KKMV    S G V +  + L LFLEF ASVFPTI  D N+ F
Sbjct: 250 KKMVKSRMSQGNVSEVSKCLSLFLEFFASVFPTIIYDCNIGF 278

[Close]

BLAST of Spg002991 vs. ExPASy TrEMBL
Match: A0A0A0KUC8 (Autophagy_act_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G022890 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.7e-72
Identity = 155/265 (58.49%), Postives = 182/265 (68.68%), Query Frame = 0


Query: 22  KERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHKQFLVLRNGQLCYLLLY 81
           +E+ +V T  +FI AG+ LV    SWSWESGD SK++S LP  KQFL+LRN         
Sbjct: 15  REKTIVFTEADFITAGNFLVGNSDSWSWESGDLSKQKSNLPSDKQFLILRNAPCLR---- 74

Query: 82  YPFRGFSYEEEYVIVGGGDEDDDQQLIRRNPKSMTEDNLLSTEELSGLNMNLKTPIPIIH 141
              R FSY+EEYVIVG   ED+ + L     +S  EDNLLST+EL         PI II 
Sbjct: 75  ---RAFSYKEEYVIVGSDGEDNARLL----SESKDEDNLLSTKELP--KPGTMKPITIIP 134

Query: 142 SYFHVNNKED--IPDMAKPIRIYPATQQSTHLVVHEPEDNIFPRTYDISITYDKDHQTPH 201
           SYF V+ KED  +P M K I++  ATQ+STH VVH+ ED I  RTYDISITYD DHQT  
Sbjct: 135 SYFAVDKKEDLPLPYMEKSIKLCSATQESTHRVVHDSEDIICLRTYDISITYDNDHQTFR 194

Query: 202 IWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIEDHPYLFGKHASVHPRVHGVAVKKMVD 261
            W TGYHE+G  LQPELIFEDV+Q+HVGKTV+IEDHPYL GKHASVHP++HG AVKKMV 
Sbjct: 195 FWLTGYHEDGRPLQPELIFEDVRQSHVGKTVNIEDHPYLPGKHASVHPKIHGAAVKKMVK 254

Query: 262 DWTSCGIVPQADRYLFLFLEFVASV 285
              S G VP+  + L LFLEFVASV
Sbjct: 255 CRMSQGNVPEVSKCLSLFLEFVASV 266

[Close]

BLAST of Spg002991 vs. ExPASy TrEMBL
Match: A0A371HBV7 (Autophagy-related protein 3 (Fragment) OS=Mucuna pruriens OX=157652 GN=ATG3 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 9.5e-71
Identity = 150/304 (49.34%), Postives = 192/304 (63.16%), Query Frame = 0


Query: 6   RGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHK 65
           +G  E++        FKE+  VL+V EF+ AGD LVA+CP+WSWESG+ SKR+ YLPP K
Sbjct: 55  KGTVERITGPRTVSAFKEK-GVLSVSEFVTAGDNLVAKCPTWSWESGEPSKRKPYLPPEK 114

Query: 66  QFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGG----GDEDDDQQLIRR-NPKSMTEDNL 125
           QFL+ RN            R  S EEEY   GG     DED+D  L     PK    D  
Sbjct: 115 QFLITRNVPCLR-------RAASVEEEYEAAGGEVLLDDEDNDGWLATHGKPKETKSDED 174

Query: 126 LSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAK------PIRIYPATQQSTHLVVH 185
            +   +  L ++ K+ I  IHSY     +EDIPDM++       I   PAT QST+LV H
Sbjct: 175 ENLPSMESLEISRKSSIKQIHSYMGAEEEEDIPDMSEFEDPSNVIETDPATLQSTYLVAH 234

Query: 186 EPEDN--IFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSI 245
           EP+D+  +  RTYD+SITYDK +QTP +W TGY E+ M LQPEL+ EDV Q+H  KTV+I
Sbjct: 235 EPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKTVTI 294

Query: 246 EDHPYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDY 297
           EDHP+L GKHAS+HP  HG  +KK++D   S G+ P+ D+YLFLFL+F+ASV PTI+ DY
Sbjct: 295 EDHPHLPGKHASIHPCRHGAVMKKIIDVLMSRGVQPEVDKYLFLFLKFMASVIPTIEYDY 350

[Close]

BLAST of Spg002991 vs. ExPASy TrEMBL
Match: C6TK09 (Autophagy_act_C domain-containing protein OS=Glycine max OX=3847 GN=100787410 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.8e-70
Identity = 151/303 (49.83%), Postives = 189/303 (62.38%), Query Frame = 0


Query: 6   RGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHK 65
           +G  E++        FKE+  VL+V EF+ AGD LVA+CP+WSWESGD  KR+ YLPP K
Sbjct: 12  KGTVERITGPRTVSAFKEK-GVLSVSEFVTAGDNLVAKCPTWSWESGDPGKRKPYLPPEK 71

Query: 66  QFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGG----GDEDDDQQLIRR-NPKSMTEDNL 125
           QFL+ RN            R  S EEEY   GG     DED+D  L     PK    D  
Sbjct: 72  QFLITRNVPCLR-------RAASVEEEYEAAGGEVLLDDEDNDGWLATHGKPKETKSDED 131

Query: 126 LSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAK-----PIRIYPATQQSTHLVVHE 185
            +   +  L ++ K  I  IHSY      EDIPDMA+      +   PAT QST+LV HE
Sbjct: 132 ENLPSMESLEISKKGSIKQIHSYMGAEEGEDIPDMAEFDDTSNVENDPATLQSTYLVAHE 191

Query: 186 PEDN--IFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTVSIE 245
           P+D+  +  RTYDISITYDK +QTP +W TGY E+ M LQPEL+ EDV Q+H  KTV+IE
Sbjct: 192 PDDDNILRTRTYDISITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKTVTIE 251

Query: 246 DHPYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKLDYN 297
           DHP+L GKHAS+HP  HG  +KK++D   S G+ P+ D+YLFLFL+F+ASV PTI+ DY 
Sbjct: 252 DHPHLPGKHASIHPCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIEYDYT 306

[Close]

BLAST of Spg002991 vs. TAIR 10
Match: AT5G61500.1 (autophagy 3 (APG3) )

HSP 1 Score: 265.0 bits (676), Expect = 7.2e-71
Identity = 149/306 (48.69%), Postives = 191/306 (62.42%), Query Frame = 0


Query: 6   RGMAEKVKSSFRTFPFKERLVVLTVPEFIAAGDCLVAQCPSWSWESGDFSKRRSYLPPHK 65
           +G  E++        FKE+  VL+V EF+ AGD LV++CP+WSWESGD SKR+ YLP  K
Sbjct: 12  KGTVERITGPRTISAFKEK-GVLSVSEFVLAGDNLVSKCPTWSWESGDASKRKPYLPSDK 71

Query: 66  QFLVLRNGQLCYLLLYYPFRGFSYEEEYVIVGG----GDEDDDQQLI---RRNPKSMTED 125
           QFL+ RN            R  S  E+Y   GG     DED+D  L    +   K   ED
Sbjct: 72  QFLITRNVPCLR-------RAASVAEDYEAAGGEVLVDDEDNDGWLATHGKPKDKGKEED 131

Query: 126 NLLSTEELSGLNMNLKTPIPIIHSYFHVNNKEDIPDMAK------PIRIYPATQQSTHLV 185
           NL S   +  L++N K  I  I +YF     +DIPDM +       +   PAT QST+LV
Sbjct: 132 NLPS---MDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDEADNVVENDPATLQSTYLV 191

Query: 186 VHEPEDN--IFPRTYDISITYDKDHQTPHIWFTGYHENGMHLQPELIFEDVKQNHVGKTV 245
            HEP+D+  +  RTYD+SITYDK +QTP +W TGY E+ M LQPEL+ EDV Q+H  KTV
Sbjct: 192 AHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLLQPELVMEDVSQDHARKTV 251

Query: 246 SIEDHPYLFGKHASVHPRVHGVAVKKMVDDWTSCGIVPQADRYLFLFLEFVASVFPTIKL 297
           +IEDHP+L GKHASVHP  HG  +KK++D   S G+ P+ D+YLFLFL+F+ASV PTI+ 
Sbjct: 252 TIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIEY 306

The following BLAST results are available for this feature:

BLAST of Spg002991 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141116.1	3.0e-79	54.82	autophagy-related protein 3 [Momordica charantia]	[more]
XP_004146945.2	4.8e-77	58.84	autophagy-related protein 3 [Cucumis sativus] >KAE8649136.1 hypothetical protein...	[more]
XP_008453898.1	2.7e-75	56.74	PREDICTED: autophagy-related protein 3 isoform X1 [Cucumis melo]	[more]
XP_038879221.1	1.8e-71	56.16	LOW QUALITY PROTEIN: autophagy-related protein 3 [Benincasa hispida]	[more]
XP_012090471.1	1.5e-70	50.99	autophagy-related protein 3 isoform X2 [Jatropha curcas]	[more]

BLAST of Spg002991 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WWQ1	1.0e-69	48.69	Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2	[more]
Q550A8	1.3e-40	34.36	Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV...	[more]
Q6PFS7	2.2e-32	33.12	Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1	[more]
Q5I0S6	4.2e-31	33.66	Ubiquitin-like-conjugating enzyme ATG3 OS=Xenopus tropicalis OX=8364 GN=atg3 PE=...	[more]
Q9CPX6	5.4e-31	32.79	Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=...	[more]

BLAST of Spg002991 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CI24	1.5e-79	54.82	autophagy-related protein 3 OS=Momordica charantia OX=3673 GN=LOC111011588 PE=4 ...	[more]
A0A1S3BWT8	1.3e-75	56.74	autophagy-related protein 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494478 P...	[more]
A0A0A0KUC8	1.7e-72	58.49	Autophagy_act_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G02...	[more]
A0A371HBV7	9.5e-71	49.34	Autophagy-related protein 3 (Fragment) OS=Mucuna pruriens OX=157652 GN=ATG3 PE=4...	[more]
C6TK09	2.8e-70	49.83	Autophagy_act_C domain-containing protein OS=Glycine max OX=3847 GN=100787410 PE...	[more]

BLAST of Spg002991 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61500.1	7.2e-71	48.69	autophagy 3 (APG3)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007135	Ubiquitin-like-conjugating enzyme Atg3/Atg10	PFAM	PF03987	Autophagy_act_C	coord: 32..292 e-value: 2.6E-40 score: 138.6
IPR007135	Ubiquitin-like-conjugating enzyme Atg3/Atg10	PANTHER	PTHR12866	UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3	coord: 6..296
None	No IPR available	PANTHER	PTHR12866:SF2	UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3	coord: 6..296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002991.1	Spg002991.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006914	autophagy
biological_process	GO:0006996	organelle organization
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0019787	ubiquitin-like protein transferase activity