Spg002967 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg002967 vs. NCBI nr
Match: KAG7013188.1 (K(+) efflux antiporter 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 924.9 bits (2389), Expect = 3.3e-265
Identity = 505/529 (95.46%), Postives = 511/529 (96.60%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M++RF MARRGGAIGSWLC V+VLISAQIHVSARSDKEIRERFYGNLINSTAP SGDGSI
Sbjct: 1   MLHRFFMARRGGAIGSWLCIVLVLISAQIHVSARSDKEIRERFYGNLINSTAPISGDGSI 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEF DNDLPEGSGGSSFN+SVADQEAELETVAKITHEKGKKNDSQKA    
Sbjct: 61  AQMFDKVLEKEFPDNDLPEGSGGSSFNNSVADQEAELETVAKITHEKGKKNDSQKA---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLT AS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTVASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAV TVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVVTVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. NCBI nr
Match: XP_022945950.1 (K(+) efflux antiporter 5 [Cucurbita moschata] >XP_022945951.1 K(+) efflux antiporter 5 [Cucurbita moschata] >KAG6574128.1 K(+) efflux antiporter 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 924.9 bits (2389), Expect = 3.3e-265
Identity = 505/529 (95.46%), Postives = 511/529 (96.60%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M++RF MARRGGAIGSWLC V+VLISAQIHVSARSDKEIRERFYGNLINSTAP SGDGSI
Sbjct: 1   MLHRFFMARRGGAIGSWLCIVLVLISAQIHVSARSDKEIRERFYGNLINSTAPISGDGSI 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEF DNDLPEGSGGSSFN+SVADQEAELETVAKITHEKGKKNDSQKA    
Sbjct: 61  AQMFDKVLEKEFPDNDLPEGSGGSSFNNSVADQEAELETVAKITHEKGKKNDSQKA---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLT AS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTVASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAV TVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVVTVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. NCBI nr
Match: XP_038905351.1 (K(+) efflux antiporter 5 [Benincasa hispida])

HSP 1 Score: 924.1 bits (2387), Expect = 5.7e-265
Identity = 504/529 (95.27%), Postives = 513/529 (96.98%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M +R+LM RRGGAIGSWLC V+VLISAQIHV+ARSDKEIRERFYGNLINSTAPTSGDGSI
Sbjct: 1   MGFRYLMVRRGGAIGSWLCIVVVLISAQIHVTARSDKEIRERFYGNLINSTAPTSGDGSI 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEFS+NDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKA    
Sbjct: 61  AQMFDKVLEKEFSENDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKA---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFI+EMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFINEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLTAAS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAVA VVAKAFGYSIRTSFQVG+MLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVAMVVAKAFGYSIRTSFQVGIMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. NCBI nr
Match: XP_023542332.1 (K(+) efflux antiporter 5 [Cucurbita pepo subsp. pepo] >XP_023542333.1 K(+) efflux antiporter 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919.8 bits (2376), Expect = 1.1e-263
Identity = 502/529 (94.90%), Postives = 510/529 (96.41%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M++RF MARRGGAIGSWLC V+VLISA+ HVSARSDKEIRERFYGNLINSTAP SGDGSI
Sbjct: 1   MLHRFFMARRGGAIGSWLCIVLVLISARSHVSARSDKEIRERFYGNLINSTAPISGDGSI 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEF DNDLPEGSGGS+FN+SVADQEAELETVAKITHEKGKKNDSQKA    
Sbjct: 61  AQMFDKVLEKEFPDNDLPEGSGGSNFNNSVADQEAELETVAKITHEKGKKNDSQKA---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLT AS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTVASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAV TVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVVTVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. NCBI nr
Match: XP_004148124.1 (K(+) efflux antiporter 5 [Cucumis sativus] >KGN57301.1 hypothetical protein Csa_009498 [Cucumis sativus])

HSP 1 Score: 914.4 bits (2362), Expect = 4.5e-262
Identity = 498/529 (94.14%), Postives = 510/529 (96.41%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M +RF MARRGGAIGS+LC ++VLIS+ IHV+ARSDKEIRERFYGNLINSTAPTSGDGS 
Sbjct: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKA    
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKA---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQ QDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVA+FGGFLQIIIFMFLCGI+AMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLTAAS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVM+STTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMVSTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAVAT+VAKAFGY IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. ExPASy Swiss-Prot
Match: Q8VYR9 (K(+) efflux antiporter 5 OS=Arabidopsis thaliana OX=3702 GN=KEA5 PE=2 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.4e-218
Identity = 411/528 (77.84%), Postives = 472/528 (89.39%), Query Frame = 0

Query: 20  TVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSIAQMFDKVLEKEFSDNDLPE 79
           T ++L+   +  SARSD+E RERFYGN++NSTAP +G+GSIA+MFD+VLEKEFS+ND PE
Sbjct: 10  TFLLLLGTSL--SARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFSENDSPE 69

Query: 80  GSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLLDSGTRAFQFQDVF 139
           GS G+SFNSSVADQ+AE+ETVAK+THEKGK+ND+Q+           ++GTR FQ QDVF
Sbjct: 70  GSDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQE-----------NNGTRPFQLQDVF 129

Query: 140 SLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCL 199
           SLENE+SDD+ TLIDKK+NVFVMSNKKSKYP+LQVDLRLISDLVV+IV AAIGGI+FSCL
Sbjct: 130 SLENEDSDDM-TLIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCL 189

Query: 200 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVA 259
           GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFS+TKLKVVG VA
Sbjct: 190 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVA 249

Query: 260 VFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQ 319
           V GG LQI++ MFLCG+ A+L GA+LSEG+FVG+FLSMSSTAVVVKFLVERNS+++LHGQ
Sbjct: 250 VLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQ 309

Query: 320 VTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAASFLSWSFVPR 379
           VTIG LI QDC VGLLFALLPVLGG++GLL G+ISMGKLLL+LS+YLT AS L+WSFVPR
Sbjct: 310 VTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPR 369

Query: 380 FLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFGQHTLD 439
           FLKLM+QLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STT+F QHTL+
Sbjct: 370 FLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLE 429

Query: 440 QVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVVAKAFGYSIR 499
           QVEPIRNLFAALFLSSIGMLI+VHFLW+H+DILLASV+LV+ +KTA+A VV KAF Y++R
Sbjct: 430 QVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMR 489

Query: 500 TSFQVGVMLAQIGEFAFVLLSRASNLH---------LIGVTSLSIYLT 539
            SF VGV+LAQIGEFAFVLLSRASNLH         L+G T+LS+  T
Sbjct: 490 ISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTT 523

BLAST of Spg002967 vs. ExPASy Swiss-Prot
Match: B5X0N6 (K(+) efflux antiporter 6 OS=Arabidopsis thaliana OX=3702 GN=KEA6 PE=2 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.3e-166
Identity = 335/526 (63.69%), Postives = 406/526 (77.19%), Query Frame = 0

Query: 33  ARSDKEIRERFYGNLINSTAPTSG----------DGSIAQMFDKVLEKEFSDNDLPEGSG 92
           A SD ++ +    N  +S A  +           +GS A + D+ LEKEF+++D  E + 
Sbjct: 37  AISDSDLLDETVANSSSSVASLNASSSSSLVKPKEGSFADIIDRALEKEFNESDQNEVAD 96

Query: 93  GSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLLDSGTRAFQFQDVFSLE 152
             SFN+SVA Q+A LETVA++  +  KKN++++ K               FQ  DVF+L 
Sbjct: 97  PGSFNNSVAGQQAVLETVARV--KSTKKNETKEEK--------------RFQLHDVFNLN 156

Query: 153 NE-ESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQ 212
           N+  ++D  TLID+KDNVF++SN KSKYPVLQ+DLRLISDLVVVIVSA  GGI F+C GQ
Sbjct: 157 NDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQ 216

Query: 213 PVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAVF 272
           PVI GYLLAGSIIGPGGL FISEMVQVETVAQFGVVFLLFALGLEFS  KLKVV +VAV 
Sbjct: 217 PVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFSTAKLKVVRSVAVL 276

Query: 273 GGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVT 332
           GG LQI++FMFLCGI   L G K SEGVFVG+FLSMSSTAVV+KFL+E+NS+N+LHGQVT
Sbjct: 277 GGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLMEKNSTNSLHGQVT 336

Query: 333 IGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAASFLSWSFVPRFL 392
           IG LILQDCAVGLLFALLPVL G++G++ GM+S+GK++++L  +L   S LS + +P  L
Sbjct: 337 IGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVLSILSRTCIPWLL 396

Query: 393 KLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFGQHTLDQV 452
           KLM+ LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF AGVMISTTD  +HTL+Q+
Sbjct: 397 KLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAEHTLEQI 456

Query: 453 EPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVVAKAFGYSIRTS 512
           EPIRNLFAALFL+SIGML++VHFLW+H+DILLASV+LV+ +KT + T V K FGY+ +T+
Sbjct: 457 EPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTTVVKGFGYNNKTA 516

Query: 513 FQVGVMLAQIGEFAFVLLSRASNLHLI---------GVTSLSIYLT 539
             VG+ LAQIGEFAFVLLSRASNLHLI         G T+LS+  T
Sbjct: 517 LLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTT 546

BLAST of Spg002967 vs. ExPASy Swiss-Prot
Match: Q9ZUN3 (K(+) efflux antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=KEA4 PE=2 SV=2)

HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-164
Identity = 340/537 (63.31%), Postives = 400/537 (74.49%), Query Frame = 0

Query: 18  LCTVMVLISAQIHVSARSDKE----IRERFYGNLI--NSTAPTSGDGSIAQMFDKVLEKE 77
           +CT     S     SA SD E    +     G ++  N+T+    + S A M D+ LEKE
Sbjct: 24  ICTFFFFFS--FAYSAESDNETDSVVTREINGTVVESNATSAKPREDSFADMIDRALEKE 83

Query: 78  FSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLLDSGTR 137
           F DND  E     SFN+SVADQ+A LETVA++   K KKN+                 T+
Sbjct: 84  FPDNDQNEVPDPGSFNNSVADQQAVLETVARV---KPKKNE-----------------TK 143

Query: 138 AFQFQDVFSLENEES-DDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAA 197
             + +  F+L+NE   +D   LID+KDNVF+MSN KSKYPVLQ+DLRLISDLVVVIVSA 
Sbjct: 144 TKEEKSFFNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRLISDLVVVIVSAT 203

Query: 198 IGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLT 257
            GGI F+C GQPVI GYLLAGSIIGPGGL F+SEMVQVETVAQFGV+FLLFALGLEFS  
Sbjct: 204 CGGIAFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSAA 263

Query: 258 KLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVER 317
           KL+VV AVA+ GG LQI +FM L GI A L G KL+EG+FVG+FLSMSSTAVV+KFL+ER
Sbjct: 264 KLRVVRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMSSTAVVLKFLMER 323

Query: 318 NSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAAS 377
           NS + LHGQ+T+GTLILQDCAVGLLFALLPVLGG +G+L G++SM K L +L  +L A  
Sbjct: 324 NSISALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKSLAILIAFLGALF 383

Query: 378 FLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMIST 437
            LS ++VP FLKLM  LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF AGVMIST
Sbjct: 384 VLSRTWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIST 443

Query: 438 TDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVV 497
           TD  QHTL+QVEPIRN FAALFL+SIGMLIH+HFLW+H+DILLA+V+LV+ +KT V  +V
Sbjct: 444 TDLAQHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLLVIVIKTVVVAIV 503

Query: 498 AKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLI---------GVTSLSIYLT 539
            K FGY+ +T+  VG+ LAQIGEFAFVLLSRASNLHLI         G T+LS+  T
Sbjct: 504 VKVFGYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLGTTALSLVTT 538

BLAST of Spg002967 vs. ExPASy Swiss-Prot
Match: Q1C2S9 (Glutathione-regulated potassium-efflux system protein KefB OS=Yersinia pestis bv. Antiqua (strain Antiqua) OX=360102 GN=kefB PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.2e-33
Identity = 117/362 (32.32%), Postives = 199/362 (54.97%), Query Frame = 0

Query: 178 LISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVV 237
           L++ ++V + +A +   I   LG   ++GYL+AG  IGP GL FI ++ ++   ++ GVV
Sbjct: 6   LLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVV 65

Query: 238 FLLFALGLEFSLTKLKVVGAVAVFG-GFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLS 297
           FL+F +GLE +  KL  +   ++FG G  Q++I   + G +   +       V  G  L+
Sbjct: 66  FLMFIIGLELNPAKLWQLRR-SIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLA 125

Query: 298 MSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMG 357
           MSSTA+ ++ + E+  +    GQ+    L+ QD AV    AL+P+L G+ G     + +G
Sbjct: 126 MSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPILAGNEGGANDWVKIG 185

Query: 358 KLLLVLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLS 417
             +   +  L    +L     P F  +   ++S   E++  AA+   L SA   D LGLS
Sbjct: 186 LKIAAFAGMLIGGRYL---LRPLFRYI---VASGVREVFTAAALLVVLGSALFMDALGLS 245

Query: 418 LELGSFVAGVMISTTDFGQHTLD-QVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLAS 477
           + LG+F+AG++++ ++F QH L+  +EP + L   LF  S+GM + +  L++HL  +L  
Sbjct: 246 MALGTFIAGILLAESEF-QHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLG 305

Query: 478 VMLVVFVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGVTSLSI 537
           V+ +VF+K+A+   +A+ FG       Q   +L+Q GEFAFVL S A +  ++    L++
Sbjct: 306 VLALVFIKSAILYGLARVFGLRRSVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNAEQLAL 359

BLAST of Spg002967 vs. ExPASy Swiss-Prot
Match: Q8ZJC4 (Glutathione-regulated potassium-efflux system protein KefB OS=Yersinia pestis OX=632 GN=kefB PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.2e-33
Identity = 117/362 (32.32%), Postives = 199/362 (54.97%), Query Frame = 0

Query: 178 LISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVV 237
           L++ ++V + +A +   I   LG   ++GYL+AG  IGP GL FI ++ ++   ++ GVV
Sbjct: 6   LLTAVLVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVV 65

Query: 238 FLLFALGLEFSLTKLKVVGAVAVFG-GFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLS 297
           FL+F +GLE +  KL  +   ++FG G  Q++I   + G +   +       V  G  L+
Sbjct: 66  FLMFIIGLELNPAKLWQLRR-SIFGVGAGQVVITAAVLGALLYFTQFAWQAAVIGGVGLA 125

Query: 298 MSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMG 357
           MSSTA+ ++ + E+  +    GQ+    L+ QD AV    AL+P+L G+ G     + +G
Sbjct: 126 MSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPILAGNEGGANDWVKIG 185

Query: 358 KLLLVLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLS 417
             +   +  L    +L     P F  +   ++S   E++  AA+   L SA   D LGLS
Sbjct: 186 LKIAAFAGMLIGGRYL---LRPLFRYI---VASGVREVFTAAALLVVLGSALFMDALGLS 245

Query: 418 LELGSFVAGVMISTTDFGQHTLD-QVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLAS 477
           + LG+F+AG++++ ++F QH L+  +EP + L   LF  S+GM + +  L++HL  +L  
Sbjct: 246 MALGTFIAGILLAESEF-QHELEIAIEPFKGLLLGLFFISVGMALDLGVLFTHLLDVLLG 305

Query: 478 VMLVVFVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGVTSLSI 537
           V+ +VF+K+A+   +A+ FG       Q   +L+Q GEFAFVL S A +  ++    L++
Sbjct: 306 VLALVFIKSAILYGLARVFGLRRSVRLQFAGVLSQGGEFAFVLFSAAFSQRVLNAEQLAL 359

BLAST of Spg002967 vs. ExPASy TrEMBL
Match: A0A6J1G2F9 (K(+) efflux antiporter 5 OS=Cucurbita moschata OX=3662 GN=LOC111450040 PE=4 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.6e-265
Identity = 505/529 (95.46%), Postives = 511/529 (96.60%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M++RF MARRGGAIGSWLC V+VLISAQIHVSARSDKEIRERFYGNLINSTAP SGDGSI
Sbjct: 1   MLHRFFMARRGGAIGSWLCIVLVLISAQIHVSARSDKEIRERFYGNLINSTAPISGDGSI 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEF DNDLPEGSGGSSFN+SVADQEAELETVAKITHEKGKKNDSQKA    
Sbjct: 61  AQMFDKVLEKEFPDNDLPEGSGGSSFNNSVADQEAELETVAKITHEKGKKNDSQKA---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLT AS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTVASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAV TVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVVTVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. ExPASy TrEMBL
Match: A0A0A0LB52 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G177940 PE=4 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.2e-262
Identity = 498/529 (94.14%), Postives = 510/529 (96.41%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M +RF MARRGGAIGS+LC ++VLIS+ IHV+ARSDKEIRERFYGNLINSTAPTSGDGS 
Sbjct: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKA    
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKA---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQ QDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVA+FGGFLQIIIFMFLCGI+AMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLTAAS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVM+STTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMVSTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAVAT+VAKAFGY IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. ExPASy TrEMBL
Match: A0A6J1HVY8 (K(+) efflux antiporter 5 OS=Cucurbita maxima OX=3661 GN=LOC111467381 PE=4 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 1.8e-261
Identity = 499/523 (95.41%), Postives = 503/523 (96.18%), Query Frame = 0

Query: 7   MARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSIAQMFDK 66
           MARRGGAIGSWLC V+VLISAQIHVS RSDKEIRERFYGNLINSTAP SGDGSIAQMFDK
Sbjct: 1   MARRGGAIGSWLCIVLVLISAQIHVSPRSDKEIRERFYGNLINSTAPISGDGSIAQMFDK 60

Query: 67  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLL 126
           VLEKEF DNDLPEGS GSSFN+SVADQEAELETVAKITHEKGKKNDSQKA          
Sbjct: 61  VLEKEFPDNDLPEGSSGSSFNNSVADQEAELETVAKITHEKGKKNDSQKA---------- 120

Query: 127 DSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVI 186
            +GTRAFQFQDVFSLENEESDDV TLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVI
Sbjct: 121 -NGTRAFQFQDVFSLENEESDDVMTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVI 180

Query: 187 VSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLE 246
           VSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLE
Sbjct: 181 VSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLE 240

Query: 247 FSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKF 306
           FSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKF
Sbjct: 241 FSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKF 300

Query: 307 LVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYL 366
           LVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLLVLSVYL
Sbjct: 301 LVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYL 360

Query: 367 TAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGV 426
           T AS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGV
Sbjct: 361 TVASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGV 420

Query: 427 MISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAV 486
           MISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAV
Sbjct: 421 MISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAV 480

Query: 487 ATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
            TVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 VTVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 512

BLAST of Spg002967 vs. ExPASy TrEMBL
Match: A0A1S3AXZ1 (K(+) efflux antiporter 5 OS=Cucumis melo OX=3656 GN=LOC103484011 PE=4 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.4e-261
Identity = 498/529 (94.14%), Postives = 508/529 (96.03%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           M +RF MARR GAIGS LC V+VLIS+QIHV+ARSDKEIRERFYGNLINSTAPTSGDGS 
Sbjct: 1   MGFRFHMARRDGAIGSCLCIVVVLISSQIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQK     
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKT---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQ QDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGI+AMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGL+LGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYLTAAS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVM+STT+FGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV
Sbjct: 421 SFVAGVMVSTTEFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAVAT+VAKAFGY IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. ExPASy TrEMBL
Match: A0A6J1CII0 (K(+) efflux antiporter 5 OS=Momordica charantia OX=3673 GN=LOC111011486 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.9e-259
Identity = 495/529 (93.57%), Postives = 506/529 (95.65%), Query Frame = 0

Query: 1   MVYRFLMARRGGAIGSWLCTVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSI 60
           MVYRFLMARRGGAIGSWL  V++LISA IHVS RSD EIRERFYGNLINSTAPTSGDGSI
Sbjct: 1   MVYRFLMARRGGAIGSWLGIVVLLISAPIHVSPRSDSEIRERFYGNLINSTAPTSGDGSI 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGT 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKN+SQK     
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNESQKT---- 120

Query: 121 KTLLLLDSGTRAFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180
                  +GTRAFQ QDVFSLENE+SDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS
Sbjct: 121 -------NGTRAFQLQDVFSLENEDSDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLIS 180

Query: 181 DLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240
           DLVVVIVSAA+GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL
Sbjct: 181 DLVVVIVSAAVGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLL 240

Query: 241 FALGLEFSLTKLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSST 300
           FALGLEFSLTKLKVVGAVAV GGFLQIIIFM L GIIAMLSGAKLSEGVFVGSFLSMSST
Sbjct: 241 FALGLEFSLTKLKVVGAVAVLGGFLQIIIFMLLSGIIAMLSGAKLSEGVFVGSFLSMSST 300

Query: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360
           AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL
Sbjct: 301 AVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLL 360

Query: 361 VLSVYLTAASFLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420
           VLSVYL AAS LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG
Sbjct: 361 VLSVYLMAASVLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELG 420

Query: 421 SFVAGVMISTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVV 480
           SFVAGVM+STT+FGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVM+VV
Sbjct: 421 SFVAGVMVSTTEFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMMVV 480

Query: 481 FVKTAVATVVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 530
           FVKTAV+T+VAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG
Sbjct: 481 FVKTAVSTIVAKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIG 518

BLAST of Spg002967 vs. TAIR 10
Match: AT5G51710.1 (K+ efflux antiporter 5 )

HSP 1 Score: 760.4 bits (1962), Expect = 1.0e-219
Identity = 411/528 (77.84%), Postives = 472/528 (89.39%), Query Frame = 0

Query: 20  TVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSIAQMFDKVLEKEFSDNDLPE 79
           T ++L+   +  SARSD+E RERFYGN++NSTAP +G+GSIA+MFD+VLEKEFS+ND PE
Sbjct: 10  TFLLLLGTSL--SARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFSENDSPE 69

Query: 80  GSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLLDSGTRAFQFQDVF 139
           GS G+SFNSSVADQ+AE+ETVAK+THEKGK+ND+Q+           ++GTR FQ QDVF
Sbjct: 70  GSDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQE-----------NNGTRPFQLQDVF 129

Query: 140 SLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCL 199
           SLENE+SDD+ TLIDKK+NVFVMSNKKSKYP+LQVDLRLISDLVV+IV AAIGGI+FSCL
Sbjct: 130 SLENEDSDDM-TLIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCL 189

Query: 200 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVA 259
           GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFS+TKLKVVG VA
Sbjct: 190 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVA 249

Query: 260 VFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQ 319
           V GG LQI++ MFLCG+ A+L GA+LSEG+FVG+FLSMSSTAVVVKFLVERNS+++LHGQ
Sbjct: 250 VLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQ 309

Query: 320 VTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAASFLSWSFVPR 379
           VTIG LI QDC VGLLFALLPVLGG++GLL G+ISMGKLLL+LS+YLT AS L+WSFVPR
Sbjct: 310 VTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPR 369

Query: 380 FLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFGQHTLD 439
           FLKLM+QLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STT+F QHTL+
Sbjct: 370 FLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLE 429

Query: 440 QVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVVAKAFGYSIR 499
           QVEPIRNLFAALFLSSIGMLI+VHFLW+H+DILLASV+LV+ +KTA+A VV KAF Y++R
Sbjct: 430 QVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMR 489

Query: 500 TSFQVGVMLAQIGEFAFVLLSRASNLH---------LIGVTSLSIYLT 539
            SF VGV+LAQIGEFAFVLLSRASNLH         L+G T+LS+  T
Sbjct: 490 ISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTT 523

BLAST of Spg002967 vs. TAIR 10
Match: AT5G51710.2 (K+ efflux antiporter 5 )

HSP 1 Score: 760.4 bits (1962), Expect = 1.0e-219
Identity = 411/528 (77.84%), Postives = 472/528 (89.39%), Query Frame = 0

Query: 20  TVMVLISAQIHVSARSDKEIRERFYGNLINSTAPTSGDGSIAQMFDKVLEKEFSDNDLPE 79
           T ++L+   +  SARSD+E RERFYGN++NSTAP +G+GSIA+MFD+VLEKEFS+ND PE
Sbjct: 10  TFLLLLGTSL--SARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFSENDSPE 69

Query: 80  GSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLLDSGTRAFQFQDVF 139
           GS G+SFNSSVADQ+AE+ETVAK+THEKGK+ND+Q+           ++GTR FQ QDVF
Sbjct: 70  GSDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQE-----------NNGTRPFQLQDVF 129

Query: 140 SLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCL 199
           SLENE+SDD+ TLIDKK+NVFVMSNKKSKYP+LQVDLRLISDLVV+IV AAIGGI+FSCL
Sbjct: 130 SLENEDSDDM-TLIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCL 189

Query: 200 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVA 259
           GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFS+TKLKVVG VA
Sbjct: 190 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVA 249

Query: 260 VFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQ 319
           V GG LQI++ MFLCG+ A+L GA+LSEG+FVG+FLSMSSTAVVVKFLVERNS+++LHGQ
Sbjct: 250 VLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQ 309

Query: 320 VTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAASFLSWSFVPR 379
           VTIG LI QDC VGLLFALLPVLGG++GLL G+ISMGKLLL+LS+YLT AS L+WSFVPR
Sbjct: 310 VTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPR 369

Query: 380 FLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFGQHTLD 439
           FLKLM+QLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STT+F QHTL+
Sbjct: 370 FLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLE 429

Query: 440 QVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVVAKAFGYSIR 499
           QVEPIRNLFAALFLSSIGMLI+VHFLW+H+DILLASV+LV+ +KTA+A VV KAF Y++R
Sbjct: 430 QVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMR 489

Query: 500 TSFQVGVMLAQIGEFAFVLLSRASNLH---------LIGVTSLSIYLT 539
            SF VGV+LAQIGEFAFVLLSRASNLH         L+G T+LS+  T
Sbjct: 490 ISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTT 523

BLAST of Spg002967 vs. TAIR 10
Match: AT5G11800.1 (K+ efflux antiporter 6 )

HSP 1 Score: 587.8 bits (1514), Expect = 9.0e-168
Identity = 335/526 (63.69%), Postives = 406/526 (77.19%), Query Frame = 0

Query: 33  ARSDKEIRERFYGNLINSTAPTSG----------DGSIAQMFDKVLEKEFSDNDLPEGSG 92
           A SD ++ +    N  +S A  +           +GS A + D+ LEKEF+++D  E + 
Sbjct: 37  AISDSDLLDETVANSSSSVASLNASSSSSLVKPKEGSFADIIDRALEKEFNESDQNEVAD 96

Query: 93  GSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLLDSGTRAFQFQDVFSLE 152
             SFN+SVA Q+A LETVA++  +  KKN++++ K               FQ  DVF+L 
Sbjct: 97  PGSFNNSVAGQQAVLETVARV--KSTKKNETKEEK--------------RFQLHDVFNLN 156

Query: 153 NE-ESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQ 212
           N+  ++D  TLID+KDNVF++SN KSKYPVLQ+DLRLISDLVVVIVSA  GGI F+C GQ
Sbjct: 157 NDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQ 216

Query: 213 PVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAVF 272
           PVI GYLLAGSIIGPGGL FISEMVQVETVAQFGVVFLLFALGLEFS  KLKVV +VAV 
Sbjct: 217 PVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFSTAKLKVVRSVAVL 276

Query: 273 GGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVT 332
           GG LQI++FMFLCGI   L G K SEGVFVG+FLSMSSTAVV+KFL+E+NS+N+LHGQVT
Sbjct: 277 GGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLMEKNSTNSLHGQVT 336

Query: 333 IGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAASFLSWSFVPRFL 392
           IG LILQDCAVGLLFALLPVL G++G++ GM+S+GK++++L  +L   S LS + +P  L
Sbjct: 337 IGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVLSILSRTCIPWLL 396

Query: 393 KLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFGQHTLDQV 452
           KLM+ LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF AGVMISTTD  +HTL+Q+
Sbjct: 397 KLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAEHTLEQI 456

Query: 453 EPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVVAKAFGYSIRTS 512
           EPIRNLFAALFL+SIGML++VHFLW+H+DILLASV+LV+ +KT + T V K FGY+ +T+
Sbjct: 457 EPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTTVVKGFGYNNKTA 516

Query: 513 FQVGVMLAQIGEFAFVLLSRASNLHLI---------GVTSLSIYLT 539
             VG+ LAQIGEFAFVLLSRASNLHLI         G T+LS+  T
Sbjct: 517 LLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTT 546

BLAST of Spg002967 vs. TAIR 10
Match: AT2G19600.1 (K+ efflux antiporter 4 )

HSP 1 Score: 580.9 bits (1496), Expect = 1.1e-165
Identity = 340/537 (63.31%), Postives = 400/537 (74.49%), Query Frame = 0

Query: 18  LCTVMVLISAQIHVSARSDKE----IRERFYGNLI--NSTAPTSGDGSIAQMFDKVLEKE 77
           +CT     S     SA SD E    +     G ++  N+T+    + S A M D+ LEKE
Sbjct: 24  ICTFFFFFS--FAYSAESDNETDSVVTREINGTVVESNATSAKPREDSFADMIDRALEKE 83

Query: 78  FSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKAKYGTKTLLLLDSGTR 137
           F DND  E     SFN+SVADQ+A LETVA++   K KKN+                 T+
Sbjct: 84  FPDNDQNEVPDPGSFNNSVADQQAVLETVARV---KPKKNE-----------------TK 143

Query: 138 AFQFQDVFSLENEES-DDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAA 197
             + +  F+L+NE   +D   LID+KDNVF+MSN KSKYPVLQ+DLRLISDLVVVIVSA 
Sbjct: 144 TKEEKSFFNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRLISDLVVVIVSAT 203

Query: 198 IGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLT 257
            GGI F+C GQPVI GYLLAGSIIGPGGL F+SEMVQVETVAQFGV+FLLFALGLEFS  
Sbjct: 204 CGGIAFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIFLLFALGLEFSAA 263

Query: 258 KLKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVER 317
           KL+VV AVA+ GG LQI +FM L GI A L G KL+EG+FVG+FLSMSSTAVV+KFL+ER
Sbjct: 264 KLRVVRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMSSTAVVLKFLMER 323

Query: 318 NSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAAS 377
           NS + LHGQ+T+GTLILQDCAVGLLFALLPVLGG +G+L G++SM K L +L  +L A  
Sbjct: 324 NSISALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKSLAILIAFLGALF 383

Query: 378 FLSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMIST 437
            LS ++VP FLKLM  LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF AGVMIST
Sbjct: 384 VLSRTWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMIST 443

Query: 438 TDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVV 497
           TD  QHTL+QVEPIRN FAALFL+SIGMLIH+HFLW+H+DILLA+V+LV+ +KT V  +V
Sbjct: 444 TDLAQHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLLVIVIKTVVVAIV 503

Query: 498 AKAFGYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLI---------GVTSLSIYLT 539
            K FGY+ +T+  VG+ LAQIGEFAFVLLSRASNLHLI         G T+LS+  T
Sbjct: 504 VKVFGYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLGTTALSLVTT 538

BLAST of Spg002967 vs. TAIR 10
Match: AT1G01790.1 (K+ efflux antiporter 1 )

HSP 1 Score: 141.0 bits (354), Expect = 2.9e-33
Identity = 105/331 (31.72%), Postives = 181/331 (54.68%), Query Frame = 0

Query: 200 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVA 259
           G PV+ GYL AG +IGP GL  I  +     +A+FGVVFLLF +GLE S+ +L  +    
Sbjct: 612 GSPVL-GYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSMKKYV 671

Query: 260 VFGGFLQIIIFMFLCGIIA-MLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHG 319
              G  Q+++   + G++A  ++G      + +G+ L++SSTAVV++ L ER  S + HG
Sbjct: 672 FGLGSAQVLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHG 731

Query: 320 QVTIGTLILQDCAVGLLFALLPVLGGHNGLLLGMISMGKLLLVLSVYLTAASFLSWSFVP 379
           + +   L+ QD AV +L  L+P++  ++    G I    +   L +    A+    + + 
Sbjct: 732 RASFSVLLFQDLAVVVLLILIPLISPNSS--KGGIGFQAIAEALGLAAVKAAVAITAIIA 791

Query: 380 --RFL--KLMMQLSSQTN-ELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDF 439
             R L   +  Q++   N E++    +   L ++  + + GLS+ LG+F+AG++++ T+F
Sbjct: 792 GGRLLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEF 851

Query: 440 GQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATVVAKA 499
                  + P R L   LF  ++GM I    L S+  +++ ++ L++  KT +  ++ K 
Sbjct: 852 SLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKL 911

Query: 500 FGYSIRTSFQVGVMLAQIGEFAFVLLSRASN 525
           FG SI ++ +VG++LA  GEFAFV    A N
Sbjct: 912 FGISIISAIRVGLLLAPGGEFAFVAFGEAVN 939