Spg002817 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002817
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Endonuclease MutS2
Location	scaffold6: 468007 .. 468624 (+)
RNA-Seq Expression	Spg002817
Synteny	Spg002817

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTCCATGTAAAGTATACAATCACCAAGTTTTCTACATTAATTAAAAGTCATCTTTTAGATTCTACTCTCTTATTCTCCATTTTCTCCCTTTGTTCAGCAACATTGAGATTTTGATTATTATTCTACAATATTTTTTATCTTTCATTTTCATTTTTCACAGCAAGCAAGGCATTAAATACATTCTATCATCTTTAATGGAAGTTGTTTCATACTCTTTTTTTTTTTTTTAACAGATGTTTCTTCCTCACTTATCCAGATTATCTGATACTTTTTCTTCATATGATTACATTTTTTGCATATGACAGGAGATACAGTCAGTTTCAACTAGTCAGTCGTTGGTACTACTAGATGAAGTGAGTATTTTACTATGTTCGGGAACAGAAAAAAAACAATCATCAATTTAAAGGAAAAGAAAATAAAATAACAAATAATTATTGATAGTCTAGTTGTTTTGCGTGGTAGGTTGGTGCAGGAACCAATCCACTGGAGGGAGCTGCACTTGGGATGTCACTCCTGGAATCTTTTGCTAAATCTGGTGCTGCGTTGACAATCGCGACTACACATCATGGAGAACTTAAAACCCTAAAATATAGGTACATTATGCAAAGATAA

mRNA sequence

ATGAACTTCCATGAGATACAGTCAGTTTCAACTAGTCAGTCGTTGGTACTACTAGATGAAGTTGGTGCAGGAACCAATCCACTGGAGGGAGCTGCACTTGGGATGTCACTCCTGGAATCTTTTGCTAAATCTGGTGCTGCGTTGACAATCGCGACTACACATCATGGAGAACTTAAAACCCTAAAATATAGGTACATTATGCAAAGATAA

Coding sequence (CDS)

ATGAACTTCCATGAGATACAGTCAGTTTCAACTAGTCAGTCGTTGGTACTACTAGATGAAGTTGGTGCAGGAACCAATCCACTGGAGGGAGCTGCACTTGGGATGTCACTCCTGGAATCTTTTGCTAAATCTGGTGCTGCGTTGACAATCGCGACTACACATCATGGAGAACTTAAAACCCTAAAATATAGGTACATTATGCAAAGATAA

Protein sequence

MNFHEIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYRYIMQR

Homology

[Close]

BLAST of Spg002817 vs. NCBI nr
Match: XP_022985051.1 (uncharacterized protein LOC111483140 isoform X2 [Cucurbita maxima])

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 543 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 602

Query: 65  YIM 68
            ++
Sbjct: 603 SLI 605

[Close]

BLAST of Spg002817 vs. NCBI nr
Match: XP_022922841.1 (uncharacterized protein LOC111430703 isoform X2 [Cucurbita moschata])

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 543 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 602

Query: 65  YIM 68
            ++
Sbjct: 603 SLI 605

[Close]

BLAST of Spg002817 vs. NCBI nr
Match: XP_022985054.1 (uncharacterized protein LOC111483140 isoform X5 [Cucurbita maxima])

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 523 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 582

Query: 65  YIM 68
            ++
Sbjct: 583 SLI 585

[Close]

BLAST of Spg002817 vs. NCBI nr
Match: XP_022922840.1 (uncharacterized protein LOC111430703 isoform X1 [Cucurbita moschata])

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 545 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 604

Query: 65  YIM 68
            ++
Sbjct: 605 SLI 607

[Close]

BLAST of Spg002817 vs. NCBI nr
Match: XP_022985050.1 (uncharacterized protein LOC111483140 isoform X1 [Cucurbita maxima])

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 545 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 604

Query: 65  YIM 68
            ++
Sbjct: 605 SLI 607

[Close]

BLAST of Spg002817 vs. ExPASy Swiss-Prot
Match: Q67QE3 (Endonuclease MutS2 OS=Symbiobacterium thermophilum (strain T / IAM 14863) OX=292459 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.8e-09
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 2   NFHEIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTL 61
           N   I       +LVLLDE+GAGT+P EGAAL MS+LE   K GA  T+ATTH+ ELKT 
Sbjct: 392 NIIRILDALEGPALVLLDELGAGTDPTEGAALAMSILEHLHKRGAK-TVATTHYSELKTY 451

Query: 62  KY 64
            Y
Sbjct: 452 AY 452

[Close]

BLAST of Spg002817 vs. ExPASy Swiss-Prot
Match: C1A4A9 (Endonuclease MutS2 OS=Gemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 11422 / NBRC 100505) OX=379066 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.3e-09
Identity = 33/60 (55.00%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 2   NFHEIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTL 61
           N  EI   +T+ SLVL+DE+G+GT+P EGAALG ++LE+  + G  LT+ATTH G+LK L
Sbjct: 402 NLGEILRSATTSSLVLIDELGSGTDPAEGAALGGAILETLTRRG-TLTLATTHLGQLKLL 460

[Close]

BLAST of Spg002817 vs. ExPASy Swiss-Prot
Match: P73625 (Endonuclease MutS2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.3e-09
Identity = 33/51 (64.71%), Postives = 40/51 (78.43%), Query Frame = 0


Query: 14  SLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 65
           SLVLLDEVGAGT+P EG+AL ++LL   A     LT+ATTH+GELK LKY+
Sbjct: 441 SLVLLDEVGAGTDPTEGSALAIALLRHLADQ-PCLTVATTHYGELKALKYQ 490

[Close]

BLAST of Spg002817 vs. ExPASy Swiss-Prot
Match: A5D0W6 (Endonuclease MutS2 OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 4.0e-09
Identity = 34/58 (58.62%), Postives = 41/58 (70.69%), Query Frame = 0


Query: 2   NFHEIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELK 60
           N  EI   +   SLVLLDE+GAGT+P EGAAL  S+LE    +GA  T+ATTH+GELK
Sbjct: 394 NIVEIIGKAGPDSLVLLDELGAGTDPAEGAALAQSILEKLHSAGAK-TVATTHYGELK 450

[Close]

BLAST of Spg002817 vs. ExPASy Swiss-Prot
Match: C3KTI4 (Endonuclease MutS2 OS=Clostridium botulinum (strain 657 / Type Ba4) OX=515621 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.2e-09
Identity = 34/63 (53.97%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 2   NFHEIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTL 61
           N  EI   +   SLVL DE+GAGT+P EGAAL +S+LE+  K GA + IATTH+ ELK  
Sbjct: 395 NIVEIMDKADENSLVLFDELGAGTDPTEGAALAISILENLRKRGAKI-IATTHYSELKAY 454

Query: 62  KYR 65
             R
Sbjct: 455 ALR 456

[Close]

BLAST of Spg002817 vs. ExPASy TrEMBL
Match: A0A6J1JC76 (uncharacterized protein LOC111483140 isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111483140 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.6e-22
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 523 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 582

Query: 65  YIM 68
            ++
Sbjct: 583 SLI 585

[Close]

BLAST of Spg002817 vs. ExPASy TrEMBL
Match: A0A6J1J709 (uncharacterized protein LOC111483140 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483140 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.6e-22
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 543 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 602

Query: 65  YIM 68
            ++
Sbjct: 603 SLI 605

[Close]

BLAST of Spg002817 vs. ExPASy TrEMBL
Match: A0A6J1JA95 (uncharacterized protein LOC111483140 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483140 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.6e-22
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 545 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 604

Query: 65  YIM 68
            ++
Sbjct: 605 SLI 607

[Close]

BLAST of Spg002817 vs. ExPASy TrEMBL
Match: A0A6J1E9Y4 (uncharacterized protein LOC111430703 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430703 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.6e-22
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 545 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 604

Query: 65  YIM 68
            ++
Sbjct: 605 SLI 607

[Close]

BLAST of Spg002817 vs. ExPASy TrEMBL
Match: A0A6J1E586 (uncharacterized protein LOC111430703 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430703 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.6e-22
Identity = 59/63 (93.65%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKYR 64
           EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAK GAALTIATTHHGELKTLKYR
Sbjct: 543 EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKCGAALTIATTHHGELKTLKYR 602

Query: 65  YIM 68
            ++
Sbjct: 603 SLI 605

[Close]

BLAST of Spg002817 vs. TAIR 10
Match: AT5G54090.1 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 97.8 bits (242), Expect = 3.6e-21
Identity = 50/59 (84.75%), Postives = 56/59 (94.92%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLKY 64
           EI S STS+SLVLLDEVGAGTNPLEGAALGM++LESFA+SG+ LT+ATTHHGELKTLKY
Sbjct: 507 EILSHSTSRSLVLLDEVGAGTNPLEGAALGMAILESFAESGSLLTMATTHHGELKTLKY 565

[Close]

BLAST of Spg002817 vs. TAIR 10
Match: AT1G65070.1 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 48.9 bits (115), Expect = 1.9e-06
Identity = 24/58 (41.38%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLK 63
           +I  +++  SLVLLDE+ +GT+P EG AL  S+L+ + K+   + + +TH+G+L  LK
Sbjct: 468 QILDIASENSLVLLDEICSGTDPSEGVALATSILQ-YIKNRVNVAVVSTHYGDLSRLK 524

[Close]

BLAST of Spg002817 vs. TAIR 10
Match: AT1G65070.2 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 48.9 bits (115), Expect = 1.9e-06
Identity = 24/58 (41.38%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 5   EIQSVSTSQSLVLLDEVGAGTNPLEGAALGMSLLESFAKSGAALTIATTHHGELKTLK 63
           +I  +++  SLVLLDE+ +GT+P EG AL  S+L+ + K+   + + +TH+G+L  LK
Sbjct: 468 QILDIASENSLVLLDEICSGTDPSEGVALATSILQ-YIKNRVNVAVVSTHYGDLSRLK 524

The following BLAST results are available for this feature:

BLAST of Spg002817 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985051.1	1.1e-21	93.65	uncharacterized protein LOC111483140 isoform X2 [Cucurbita maxima]	[more]
XP_022922841.1	1.1e-21	93.65	uncharacterized protein LOC111430703 isoform X2 [Cucurbita moschata]	[more]
XP_022985054.1	1.1e-21	93.65	uncharacterized protein LOC111483140 isoform X5 [Cucurbita maxima]	[more]
XP_022922840.1	1.1e-21	93.65	uncharacterized protein LOC111430703 isoform X1 [Cucurbita moschata]	[more]
XP_022985050.1	1.1e-21	93.65	uncharacterized protein LOC111483140 isoform X1 [Cucurbita maxima]	[more]

BLAST of Spg002817 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67QE3	1.8e-09	56.45	Endonuclease MutS2 OS=Symbiobacterium thermophilum (strain T / IAM 14863) OX=292...	[more]
C1A4A9	2.3e-09	55.00	Endonuclease MutS2 OS=Gemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 114...	[more]
P73625	2.3e-09	64.71	Endonuclease MutS2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN...	[more]
A5D0W6	4.0e-09	58.62	Endonuclease MutS2 OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10...	[more]
C3KTI4	5.2e-09	53.97	Endonuclease MutS2 OS=Clostridium botulinum (strain 657 / Type Ba4) OX=515621 GN...	[more]

BLAST of Spg002817 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JC76	5.6e-22	93.65	uncharacterized protein LOC111483140 isoform X5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1J709	5.6e-22	93.65	uncharacterized protein LOC111483140 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1JA95	5.6e-22	93.65	uncharacterized protein LOC111483140 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1E9Y4	5.6e-22	93.65	uncharacterized protein LOC111430703 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1E586	5.6e-22	93.65	uncharacterized protein LOC111430703 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Spg002817 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54090.1	3.6e-21	84.75	DNA mismatch repair protein MutS, type 2	[more]
AT1G65070.1	1.9e-06	41.38	DNA mismatch repair protein MutS, type 2	[more]
AT1G65070.2	1.9e-06	41.38	DNA mismatch repair protein MutS, type 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..67 e-value: 1.1E-17 score: 65.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 8..66
IPR000432	DNA mismatch repair protein MutS, C-terminal	PFAM	PF00488	MutS_V	coord: 5..63 e-value: 1.5E-10 score: 41.5
IPR000432	DNA mismatch repair protein MutS, C-terminal	PROSITE	PS00486	DNA_MISMATCH_REPAIR_2	coord: 14..30
IPR045076	DNA mismatch repair MutS family	PANTHER	PTHR11361	DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER	coord: 4..63
None	No IPR available	PANTHER	PTHR11361:SF132	DNA MISMATCH REPAIR PROTEIN MUTS, TYPE 2	coord: 4..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002817.1	Spg002817.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006298	mismatch repair
biological_process	GO:0045910	negative regulation of DNA recombination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0030983	mismatched DNA binding