Spg002815 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002815
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	EKC/KEOPS complex, subunit Pcc1
Location	scaffold6: 564403 .. 565867 (+)
RNA-Seq Expression	Spg002815
Synteny	Spg002815

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAGAATTTGAAGCAATGGCTCCGACAGCTTCCGTCGCTTCTGATGGAGAGGCCAAATGGGAATTCAGCTGGTTGCTCTCTCTCTCTTTAAGCCCATAATTGCATAAATTACAAGTTTTTCTTCGATGCTATGGCGTGCTTTTAAATCAGATTGTTTATTGATGTTGCTGTGTTGGCATTTGGGTAGCTTGACTCTTTGGCTTTATCTTGTTTCTCTTGTGATTAGTCCGTTTAGTTCTGTCTCCAGTTCACTGCAATTTCCACAAAGGAAATGAGACAATTGTGATGTTGATGATGATCATGTTCTTGCTCCCGCAACCCATTTTTTTACCCTGATTTTTGTTTTCCTCAATCCGGAGTTTTTTTTACCAGAATAATGTCATGAAAGCTCAAATATTCCATTGAATTCTCAACTGTTGGAAGGGATGGACATGGGCCTTTTTAAACTAGGCAAAAGGTTTCTTCTTTTGCTGTTGTTCCTGGAAGCCAATGTTGTTTCTGGGTCTTGTCAGTGTTTTCAACAATTGCTATTGAATCCTAAATGGTCATTGTAGTTGCTTCATTCTTTAAATGGTCAAAAATTGTGTAGGATCATACATTGGAAACATGCAGTTTAAATCATTGTCAGCAATCTTTCTTCTTTTCTAGATTGCCTTCCTAGAGACAGGACAAGTAATTTGTTTCTTTTTCCAAGTGTTGAATCTGGTGTGCTCTCTGCAGTGACTTTGAAGTCGATTATGAGTCTGAGAAGAAAGCTTCAATTGTCTATAATGCTTTAGTCGTCGATAAAGAGGTGCTTCTCGCTGGTATCAATCTTCACATTGTGCTCCATAGTTGACTTGTTTCTGATTTGATTTTTGTGCTAAAGTACAGTTGCAGCCAGACAAGGTTAAAAGGGAGATGACAGTTTCTGATGGAAAACTTTCAGTGTAAGCCTCTCCTGCTCTTTGGATTCCCAGTTTTGAATGTACTTTTGAGTTTTGATTGTATATAGATACCACAATAGCTCATTACAGGGTTCTTTGGAACATTAGAACCGCTGCTGCATTCGATCATATTTTGGAATTGTCATTAGCTGCCTGGTTTTTTCATGCAGACACTTTGAGGCAGTGGAAGCAAGATTTCTGCGTGCATCATACAGTGCTTTTGTGGATGTGCTTACTCTTGCCACAAAAACAATCGAAGATTTTGGACAAGGAGTGGATTTCTGATAGCCTCACTGATCTATTTACATGTTCCTCAAAGATCAAGTGCCATTTTTTGCTTAAAGTAGCTGTGATTTTAATTTACATCTGATCTGTACTCTTATTACAATATGATTTGTAGTAGTTTGATGGAGTGGGAAGGTCTTCATTCTGCCCCCATCATTCGAACATTGAGATCTAACAAGATAACATTTACAATACACATGTTGTGCTATATTACTCCTTACATTCAGCCATACGATTACTTGATATGTCAGTTT

mRNA sequence

ATGGCTCCGACAGCTTCCGTCGCTTCTGATGGAGAGGCCAAATGGGAATTCAGCTGTGACTTTGAAGTCGATTATGAGTCTGAGAAGAAAGCTTCAATTGTCTATAATGCTTTAGTCGTCGATAAAGAGTTGCAGCCAGACAAGGTTAAAAGGGAGATGACAGTTTCTGATGGAAAACTTTCAGTACACTTTGAGGCAGTGGAAGCAAGATTTCTGCGTGCATCATACAGTGCTTTTGTGGATGTGCTTACTCTTGCCACAAAAACAATCGAAGATTTTGGACAAGGAGTGGATTTCTGA

Coding sequence (CDS)

ATGGCTCCGACAGCTTCCGTCGCTTCTGATGGAGAGGCCAAATGGGAATTCAGCTGTGACTTTGAAGTCGATTATGAGTCTGAGAAGAAAGCTTCAATTGTCTATAATGCTTTAGTCGTCGATAAAGAGTTGCAGCCAGACAAGGTTAAAAGGGAGATGACAGTTTCTGATGGAAAACTTTCAGTACACTTTGAGGCAGTGGAAGCAAGATTTCTGCGTGCATCATACAGTGCTTTTGTGGATGTGCTTACTCTTGCCACAAAAACAATCGAAGATTTTGGACAAGGAGTGGATTTCTGA

Protein sequence

MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKLSVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF

Homology

[Close]

BLAST of Spg002815 vs. NCBI nr
Match: XP_038895564.1 (uncharacterized protein LOC120083771 [Benincasa hispida])

HSP 1 Score: 184.9 bits (468), Expect = 3.4e-43
Identity = 92/99 (92.93%), Postives = 97/99 (97.98%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNAL+VDKELQPDKVKR M+VSDGKL
Sbjct: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALIVDKELQPDKVKRVMSVSDGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           S+HFEAVEARFLRAS+SAFVDVLTLATKT+EDFGQ VDF
Sbjct: 61  SIHFEAVEARFLRASFSAFVDVLTLATKTVEDFGQEVDF 99

[Close]

BLAST of Spg002815 vs. NCBI nr
Match: XP_022141079.1 (uncharacterized protein LOC111011564 [Momordica charantia])

HSP 1 Score: 184.5 bits (467), Expect = 4.4e-43
Identity = 93/98 (94.90%), Postives = 95/98 (96.94%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           MAPTASVASDGEAKWEF CDFEVDYESEKKASIVYNAL VDKELQPDKVKREM VSDGKL
Sbjct: 24  MAPTASVASDGEAKWEFRCDFEVDYESEKKASIVYNALAVDKELQPDKVKREMAVSDGKL 83

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVD 99
           SVHFEAVEARFLRAS+SAFVDVLTLATKTIEDFGQG+D
Sbjct: 84  SVHFEAVEARFLRASFSAFVDVLTLATKTIEDFGQGLD 121

[Close]

BLAST of Spg002815 vs. NCBI nr
Match: XP_022945934.1 (uncharacterized protein LOC111450028 [Cucurbita moschata] >XP_023541072.1 uncharacterized protein LOC111801345 isoform X1 [Cucurbita pepo subsp. pepo] >KAG6574248.1 hypothetical protein SDJN03_28135, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013317.1 hypothetical protein SDJN02_26076 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 184.1 bits (466), Expect = 5.8e-43
Identity = 93/99 (93.94%), Postives = 96/99 (96.97%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           MAPTASV SDG+AKWEFSCDFEVD ESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL
Sbjct: 1   MAPTASVVSDGDAKWEFSCDFEVDCESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           SVHFEAVEARFLRASYSAF+DVLTLA KTIEDFGQG+DF
Sbjct: 61  SVHFEAVEARFLRASYSAFLDVLTLAIKTIEDFGQGLDF 99

[Close]

BLAST of Spg002815 vs. NCBI nr
Match: XP_023553191.1 (uncharacterized protein LOC111810678 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.0 bits (463), Expect = 1.3e-42
Identity = 92/99 (92.93%), Postives = 94/99 (94.95%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           M PT SVASDGE  WEFSCDFEVDYESEKKASIVY ALVVDKELQPDKVKREMTVSDGKL
Sbjct: 1   MPPTPSVASDGEPNWEFSCDFEVDYESEKKASIVYKALVVDKELQPDKVKREMTVSDGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           S+HFEAVEARFLRAS+SAFVDVLTLATKTIEDFGQGVDF
Sbjct: 61  SIHFEAVEARFLRASFSAFVDVLTLATKTIEDFGQGVDF 99

[Close]

BLAST of Spg002815 vs. NCBI nr
Match: KAG6576647.1 (hypothetical protein SDJN03_24221, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 182.2 bits (461), Expect = 2.2e-42
Identity = 92/99 (92.93%), Postives = 94/99 (94.95%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           MAPT SVASDGE KWEFSCD EVDYESEKKASIVY ALVVDKELQPDKVKREMTVSDGKL
Sbjct: 1   MAPTPSVASDGEPKWEFSCDLEVDYESEKKASIVYKALVVDKELQPDKVKREMTVSDGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           S+H EAVEARFLRAS+SAFVDVLTLATKTIEDFGQGVDF
Sbjct: 61  SIHLEAVEARFLRASFSAFVDVLTLATKTIEDFGQGVDF 99

[Close]

BLAST of Spg002815 vs. ExPASy TrEMBL
Match: A0A6J1CHJ5 (uncharacterized protein LOC111011564 OS=Momordica charantia OX=3673 GN=LOC111011564 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.2e-43
Identity = 93/98 (94.90%), Postives = 95/98 (96.94%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           MAPTASVASDGEAKWEF CDFEVDYESEKKASIVYNAL VDKELQPDKVKREM VSDGKL
Sbjct: 24  MAPTASVASDGEAKWEFRCDFEVDYESEKKASIVYNALAVDKELQPDKVKREMAVSDGKL 83

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVD 99
           SVHFEAVEARFLRAS+SAFVDVLTLATKTIEDFGQG+D
Sbjct: 84  SVHFEAVEARFLRASFSAFVDVLTLATKTIEDFGQGLD 121

[Close]

BLAST of Spg002815 vs. ExPASy TrEMBL
Match: A0A6J1G2C3 (uncharacterized protein LOC111450028 OS=Cucurbita moschata OX=3662 GN=LOC111450028 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.8e-43
Identity = 93/99 (93.94%), Postives = 96/99 (96.97%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           MAPTASV SDG+AKWEFSCDFEVD ESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL
Sbjct: 1   MAPTASVVSDGDAKWEFSCDFEVDCESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           SVHFEAVEARFLRASYSAF+DVLTLA KTIEDFGQG+DF
Sbjct: 61  SVHFEAVEARFLRASYSAFLDVLTLAIKTIEDFGQGLDF 99

[Close]

BLAST of Spg002815 vs. ExPASy TrEMBL
Match: A0A6J1HX84 (uncharacterized protein LOC111467483 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467483 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.0e-41
Identity = 91/99 (91.92%), Postives = 94/99 (94.95%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           MAPTASV SDG+AKWEFSCDFEVD ESEKKASIVYNALVVDKELQPDKVKR MTVSDGKL
Sbjct: 1   MAPTASVVSDGDAKWEFSCDFEVDCESEKKASIVYNALVVDKELQPDKVKRGMTVSDGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           SVHFEAVEARFLRAS SAF+DVLTLA KTIEDFGQG+DF
Sbjct: 61  SVHFEAVEARFLRASCSAFLDVLTLAIKTIEDFGQGLDF 99

[Close]

BLAST of Spg002815 vs. ExPASy TrEMBL
Match: A0A1S3CTG0 (uncharacterized protein LOC103504146 OS=Cucumis melo OX=3656 GN=LOC103504146 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.9e-41
Identity = 87/99 (87.88%), Postives = 95/99 (95.96%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           M PTASVASDGEAKWEFSCDFEVDYESEKKASIVYNAL+VDKELQPDKVKR M++S+GKL
Sbjct: 1   MTPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALIVDKELQPDKVKRAMSISNGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           S+HFEAVEARFLRAS+S+FVDVLTLATKTIEDFG  V+F
Sbjct: 61  SIHFEAVEARFLRASFSSFVDVLTLATKTIEDFGLEVEF 99

[Close]

BLAST of Spg002815 vs. ExPASy TrEMBL
Match: A0A5D3CJ23 (EKC/KEOPS complex, subunit Pcc1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001520 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.9e-41
Identity = 87/99 (87.88%), Postives = 95/99 (95.96%), Query Frame = 0


Query: 1   MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
           M PTASVASDGEAKWEFSCDFEVDYESEKKASIVYNAL+VDKELQPDKVKR M++S+GKL
Sbjct: 1   MTPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALIVDKELQPDKVKRAMSISNGKL 60

Query: 61  SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQGVDF 100
           S+HFEAVEARFLRAS+S+FVDVLTLATKTIEDFG  V+F
Sbjct: 61  SIHFEAVEARFLRASFSSFVDVLTLATKTIEDFGLEVEF 99

[Close]

BLAST of Spg002815 vs. TAIR 10
Match: AT5G53045.1 (unknown protein; Has 122 Blast hits to 122 proteins in 57 species: Archae - 0; Bacteria - 0; Metazoa - 46; Fungi - 30; Plants - 41; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 124.0 bits (310), Expect = 6.6e-29
Identity = 60/95 (63.16%), Postives = 76/95 (80.00%), Query Frame = 0


Query: 1  MAPTASVASDGEAKWEFSCDFEVDYESEKKASIVYNALVVDKELQPDKVKREMTVSDGKL 60
          MA T    +  +  W+FSC+ +V +ESE+ A I Y +L VDKELQPDKV+R M+VS+ KL
Sbjct: 1  MAATVPATTRSDQTWDFSCNLDVSFESEEHALIAYTSLAVDKELQPDKVRRVMSVSNNKL 60

Query: 61 SVHFEAVEARFLRASYSAFVDVLTLATKTIEDFGQ 96
          SVHFEA+EAR LRAS+SAFVDVLTLAT+TI++FGQ
Sbjct: 61 SVHFEAIEARLLRASFSAFVDVLTLATRTIQEFGQ 95

The following BLAST results are available for this feature:

BLAST of Spg002815 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895564.1	3.4e-43	92.93	uncharacterized protein LOC120083771 [Benincasa hispida]	[more]
XP_022141079.1	4.4e-43	94.90	uncharacterized protein LOC111011564 [Momordica charantia]	[more]
XP_022945934.1	5.8e-43	93.94	uncharacterized protein LOC111450028 [Cucurbita moschata] >XP_023541072.1 unchar...	[more]
XP_023553191.1	1.3e-42	92.93	uncharacterized protein LOC111810678 [Cucurbita pepo subsp. pepo]	[more]
KAG6576647.1	2.2e-42	92.93	hypothetical protein SDJN03_24221, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Spg002815 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg002815 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHJ5	2.2e-43	94.90	uncharacterized protein LOC111011564 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1G2C3	2.8e-43	93.94	uncharacterized protein LOC111450028 OS=Cucurbita moschata OX=3662 GN=LOC1114500...	[more]
A0A6J1HX84	2.0e-41	91.92	uncharacterized protein LOC111467483 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A1S3CTG0	5.9e-41	87.88	uncharacterized protein LOC103504146 OS=Cucumis melo OX=3656 GN=LOC103504146 PE=...	[more]
A0A5D3CJ23	5.9e-41	87.88	EKC/KEOPS complex, subunit Pcc1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of Spg002815 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53045.1	6.6e-29	63.16	unknown protein; Has 122 Blast hits to 122 proteins in 57 species: Archae - 0; B...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015419	CTAG/Pcc1 family	PFAM	PF09341	Pcc1	coord: 18..91 e-value: 3.0E-19 score: 69.0
IPR015419	CTAG/Pcc1 family	PANTHER	PTHR31283	EKC/KEOPS COMPLEX SUBUNIT PCC1 FAMILY MEMBER	coord: 10..95
None	No IPR available	GENE3D	3.30.310.50		coord: 7..97 e-value: 2.9E-23 score: 83.7
None	No IPR available	PANTHER	PTHR31283:SF5	GEO08993P1	coord: 10..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002815.1	Spg002815.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070525	tRNA threonylcarbamoyladenosine metabolic process
cellular_component	GO:0000408	EKC/KEOPS complex