Spg002803 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002803
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	endonuclease MutS2 isoform X1
Location	scaffold6: 457442 .. 460487 (+)
RNA-Seq Expression	Spg002803
Synteny	Spg002803

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAATCAGAAAGTTTTTGGATCCCTAATTCGCAGAAAAAGAAAAAGAACTTGAGGCCCTCAGGAAACGATGTTTGGGTACAAATAAAACCTGAGACCACAGGACGAGAAGAGGACTTTTAGACAGGGAATTGGATAAATGGACCAGCCTCATTTCAATCCTCCCAGATTCTCACCCCGAAGCATCTTTGGATTATCTGTGGTGGTATCCAGACTTATCAGCAAGTTGATTTGGGAGGGCAACAGCCCAAAGAAGGTAAAATCTTATATATTTTCACTAGCACATGGCAGCTTAAACACTCGGCAGAATTCAAAAGAGGAATCTTAATATTTCCTTATCCCCGAACATGTGCCTTACATGTAACAAAAATGAAGAGACCTCCAGTCACCTCTTTCTTCATTGCCTGTTTGCAAAAAAGGGTTGGAACTTCCTCTTTAATTATTCTGATTTGTCTGGTGTCCACCTAAAAAAGTGGAGATGGATTTGTCAGAATTGCTCTCTGATTGGTGGCATAAAGAGAAAGCAAGGATTATATGGTCAAAAAATAAGTACTCTCCTATTTGAACTATCCTTGTGCAAATTGCAAGAAATGACGGCACTCCTTTAGTAGATTTATTACTTGGTTTGCAATGACATCAGATGCCTCATCTCAAAAATATTGTCCATCCGTACCTTTTTCTTTTTAAATTTATCCCAATACTCATTAACCTTGGCAATTGGCATTGAATCTATTTCTGAATTTCTCTGTAAACATCACAACATCCCAGTGCTCAACTCTTATTTCCTAGTTGGCAAATTCTTGTTTTTCCTTAATGGACAGGTCAATGCTCGAGCATCTTATGGTCTTTCATTTGGGGGGACATGTCCCAATTTAGTTCTACCAGAAGGGTGCAACTCTTGTATCACTAATGTCTGCTTATCAGGAGACCGAACATCTGAGGCATCAAACCTGAAGAAGAATGAATGGGTCCTCTATTTACCTAATGCCCATCACCCTTTACTACTACAGCAATATAGAGTAAATTTGGAGAATGCCAAGAGGGATGTCAGAAATGCTTTTACTGTAAGCTATACTGAGTTTGATATCATGGAAAGCAGTTATTTAGTAAATTGAGGCTGTTCTGAGTCATCATTTTTCTCCTAGGAGATGGGGAGAAAACTTCCTGGGGGGAATATGTCATGGAAAGAAAAAGAAGTTGTAGATATTTCGTTCTTAAAAATGAAGGTAGTTTTGAATGTAATTGTGTGTCAATTGATAAAATCTACTTGCATTATTCTTTAGTGTATTCAATTTTTTTTTAAAAAAAAGCGCTTATTATTAGGTTCAGAAATTGGAGCAAGCTCATCCTGTTTCGGTTGATTTTTCAATATCTCAAAGAATTCGAGTTTTAGTTATAACTGGTCCTAATACTGGGGGTAAGACAGTTTGTTTGAAAACCATTGGATTGGCAGCCATGATGGCAAAATCAGGTATGTTTCAAGAATGGTCTGCTTTTTATTATTATAATTATTATTTTTTTTTTGGTCCACAATGAATTTTTGTTTTCATTTATGTAACCAATTCAAGTCAAGCTTAGTTGCGTAAGCTTGTGACCCAAGTGGTGGTGCTGATGTGATGGAATGAGGTCTAACTTATGTAACCAATTCAATTCTAGTTTGTAATGAGCTATATTAAATTATTGTTATCCTAAGAGCTGCAGAGCAATATTACCTATACAGTGAATCGAAAATGTGTGATATTAGAAGCCCTGATCCATTATGCAGATATCATTATATTTACATCAATTTTATCGCTTCTGATATTGTCTTTTTACAAGAATATTTAAATGACTTTGTTGTCCTTTTATTCACTTTATAGGGATTCATGTCTTAGCTTCAGAGTCTGTACAAATCCCTTGGTTTGATTCTGTTTTTGCTGATATCGGTGATGAACAGTCCCTAACCCAATCTTTGTCTACCTTTTCTGGCAATTTGAGAAAAATAAGCGTAAGTAAACTTGGATACTTCATCTTGTTTCAACGTTTTCTGTGATTTCCCTACTATGCATGCAGTCTCTGTACGTGGCATAATCATTAGAGACATTCATATTTCTTAATTATGATGACCTTTTCGTGAGTTGATTAAGCTGTGGAAGTTCTTAGCTACTGATGTCTTGTGGGGGATAGTGCCTTTTTCTGATTTCATAAGTGGCTTTGGAAGTAATTCTATTTTTGTTATTATCCTTTAGTTCTTGTTTTACTTTATCGGGATCCTTTTTATTTTAGTTTGGCTCCTTTCATTTTGGTGCTTTATCATTTGAATGTCCCTTTGTATTCTTTTATTTTTCCTTAATGAAAGCTTGGATTTTCATTGAGTAGATAAATAAGTAATTATGTTCTTTCTACTTTAAGACCTGGGGATAAGAAAAATAAGTTTTATGGATAAACTCTTCATTAAATATTGCGTGGCTTTTCTTGAGTAAAATCCTTGGTAAGAAAGCTAGCTTCATCTTCACTGTTGCAAAAGGAGATTTTCAAGGTTATTTGGAAATCTAAAGTTCCAAAGAAAATTAACCTCCTTTAGATTTTATTGAATGGGAATCTTAATATTTCAGAGGGTTTGCAGCGAAAATTGCCTCATTTTGCTCTCATGCCCTCGGTTTGCTCTCTTTGTCTTGAGAATGGGGATTTTCTCAACCATCTTTTCTTTGGTTGCTCTTATTTGCTAATTGTTGGTTCAAATTATTTGAAGTTTTCAAGGTTTCTTGGGTTTTTTCATCCTCTTTGAGTGATAATTTGTCTCATCTGCCTCTTGGTTTATCCTTGCCAGAAAAATCTCATCTTTTATGGATTAATGCAATTAAAGCTCTTCTTTCGGAGGTTTGGATGGAGAGAGATTCAAGAATTTTTTAGAACAAGAGTCTTCTTTGGTTTGATTCAGTTAGGTGTCTCATTTAAAAGTTGTCTCATCTTGGTGTGTTTCATCTGAGAAGTAGACGAGACACAGTGCTCTTTTGGAGCAATCAGAAAATACCCAAGAAGTAGATGAGACACGGTGCTCTTAA

mRNA sequence

ATGGCCAATCAGAAAGTCAATGCTCGAGCATCTTATGGTCTTTCATTTGGGGGGACATGTCCCAATTTAGTTCTACCAGAAGGGTGCAACTCTTGTATCACTAATGTCTGCTTATCAGGAGACCGAACATCTGAGGCATCAAACCTGAAGAAGAATGAATGGGTCCTCTATTTACCTAATGCCCATCACCCTTTACTACTACAGCAATATAGAGTAAATTTGGAGAATGCCAAGAGGGATGTCAGAAATGCTTTTACTGAGATGGGGAGAAAACTTCCTGGGGGGAATATGTCATGGAAAGAAAAAGAAGTTGTAGATATTTCGTTCTTAAAAATGAAGGTTCAGAAATTGGAGCAAGCTCATCCTGTTTCGGTTGATTTTTCAATATCTCAAAGAATTCGAGTTTTAGTTATAACTGGTCCTAATACTGGGGGTAAGACAGTTTGTTTGAAAACCATTGGATTGGCAGCCATGATGGCAAAATCAGGGATTCATGTCTTAGCTTCAGAGTCTGTACAAATCCCTTGGTTTGATTCTGTTTTTGCTGATATCGGTGATGAACAGTCCCTAACCCAATCTTTGTCTACCTTTTCTGGCAATTTGAGAAAAATAAGCTTGATTAAGCTGTGGAAGTTCTTAGCTACTGATGTCTTGTGGGGGATAGTGCCTTTTTCTGATTTCATAAGTGGCTTTGGAATTTTCAAGGTTTCTTGGGTTTTTTCATCCTCTTTGAGTGATAATTTGTCTCATCTGCCTCTTGGTTTATCCTTGCCAGAAAAATCTCATCTTTTATGGATTAATGCAATTAAAGCTCTTCTTTCGGAGACGAGACACAGTGCTCTTTTGGAGCAATCAGAAAATACCCAAGAAGTAGATGAGACACGGTGCTCTTAA

Coding sequence (CDS)

ATGGCCAATCAGAAAGTCAATGCTCGAGCATCTTATGGTCTTTCATTTGGGGGGACATGTCCCAATTTAGTTCTACCAGAAGGGTGCAACTCTTGTATCACTAATGTCTGCTTATCAGGAGACCGAACATCTGAGGCATCAAACCTGAAGAAGAATGAATGGGTCCTCTATTTACCTAATGCCCATCACCCTTTACTACTACAGCAATATAGAGTAAATTTGGAGAATGCCAAGAGGGATGTCAGAAATGCTTTTACTGAGATGGGGAGAAAACTTCCTGGGGGGAATATGTCATGGAAAGAAAAAGAAGTTGTAGATATTTCGTTCTTAAAAATGAAGGTTCAGAAATTGGAGCAAGCTCATCCTGTTTCGGTTGATTTTTCAATATCTCAAAGAATTCGAGTTTTAGTTATAACTGGTCCTAATACTGGGGGTAAGACAGTTTGTTTGAAAACCATTGGATTGGCAGCCATGATGGCAAAATCAGGGATTCATGTCTTAGCTTCAGAGTCTGTACAAATCCCTTGGTTTGATTCTGTTTTTGCTGATATCGGTGATGAACAGTCCCTAACCCAATCTTTGTCTACCTTTTCTGGCAATTTGAGAAAAATAAGCTTGATTAAGCTGTGGAAGTTCTTAGCTACTGATGTCTTGTGGGGGATAGTGCCTTTTTCTGATTTCATAAGTGGCTTTGGAATTTTCAAGGTTTCTTGGGTTTTTTCATCCTCTTTGAGTGATAATTTGTCTCATCTGCCTCTTGGTTTATCCTTGCCAGAAAAATCTCATCTTTTATGGATTAATGCAATTAAAGCTCTTCTTTCGGAGACGAGACACAGTGCTCTTTTGGAGCAATCAGAAAATACCCAAGAAGTAGATGAGACACGGTGCTCTTAA

Protein sequence

MANQKVNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHHPLLLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIGDEQSLTQSLSTFSGNLRKISLIKLWKFLATDVLWGIVPFSDFISGFGIFKVSWVFSSSLSDNLSHLPLGLSLPEKSHLLWINAIKALLSETRHSALLEQSENTQEVDETRCS

Homology

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BLAST of Spg002803 vs. NCBI nr
Match: XP_038894074.1 (endonuclease MutS2 isoform X11 [Benincasa hispida])

HSP 1 Score: 370.5 bits (950), Expect = 1.3e-98
Identity = 183/208 (87.98%), Postives = 197/208 (94.71%), Query Frame = 0


Query: 1   MANQKVNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPN 60
           M  +KVNARASYGLSFGGTCPNL+LPEGCNS I NVCLSGD+TSEAS+ KKNEWVLYL N
Sbjct: 318 MLTEKVNARASYGLSFGGTCPNLILPEGCNSSIANVCLSGDQTSEASHSKKNEWVLYLQN 377

Query: 61  AHHPLLLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQA 120
           AHHPLLLQQYR NLENAKRDV+NAFTEMGRKLPGGNMSWKEKEVVDIS LKMKV++LE+A
Sbjct: 378 AHHPLLLQQYRENLENAKRDVQNAFTEMGRKLPGGNMSWKEKEVVDISLLKMKVEQLEKA 437

Query: 121 HPVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSV 180
            PVSVDFSIS+RI+VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+
Sbjct: 438 RPVSVDFSISRRIQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSI 497

Query: 181 FADIGDEQSLTQSLSTFSGNLRKISLIK 209
           FADIGDEQSLTQSLSTFSG+LRKIS I+
Sbjct: 498 FADIGDEQSLTQSLSTFSGHLRKISDIQ 525

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BLAST of Spg002803 vs. NCBI nr
Match: XP_038893999.1 (endonuclease MutS2 isoform X5 [Benincasa hispida])

HSP 1 Score: 368.6 bits (945), Expect = 5.0e-98
Identity = 182/206 (88.35%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 3   NQKVNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAH 62
           + KVNARASYGLSFGGTCPNL+LPEGCNS I NVCLSGD+TSEAS+ KKNEWVLYL NAH
Sbjct: 361 SDKVNARASYGLSFGGTCPNLILPEGCNSSIANVCLSGDQTSEASHSKKNEWVLYLQNAH 420

Query: 63  HPLLLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHP 122
           HPLLLQQYR NLENAKRDV+NAFTEMGRKLPGGNMSWKEKEVVDIS LKMKV++LE+A P
Sbjct: 421 HPLLLQQYRENLENAKRDVQNAFTEMGRKLPGGNMSWKEKEVVDISLLKMKVEQLEKARP 480

Query: 123 VSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFA 182
           VSVDFSIS+RI+VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+FA
Sbjct: 481 VSVDFSISRRIQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSIFA 540

Query: 183 DIGDEQSLTQSLSTFSGNLRKISLIK 209
           DIGDEQSLTQSLSTFSG+LRKIS I+
Sbjct: 541 DIGDEQSLTQSLSTFSGHLRKISDIQ 566

[Close]

BLAST of Spg002803 vs. NCBI nr
Match: XP_038894006.1 (endonuclease MutS2 isoform X6 [Benincasa hispida])

HSP 1 Score: 368.6 bits (945), Expect = 5.0e-98
Identity = 182/206 (88.35%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 3   NQKVNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAH 62
           + KVNARASYGLSFGGTCPNL+LPEGCNS I NVCLSGD+TSEAS+ KKNEWVLYL NAH
Sbjct: 359 SDKVNARASYGLSFGGTCPNLILPEGCNSSIANVCLSGDQTSEASHSKKNEWVLYLQNAH 418

Query: 63  HPLLLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHP 122
           HPLLLQQYR NLENAKRDV+NAFTEMGRKLPGGNMSWKEKEVVDIS LKMKV++LE+A P
Sbjct: 419 HPLLLQQYRENLENAKRDVQNAFTEMGRKLPGGNMSWKEKEVVDISLLKMKVEQLEKARP 478

Query: 123 VSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFA 182
           VSVDFSIS+RI+VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+FA
Sbjct: 479 VSVDFSISRRIQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSIFA 538

Query: 183 DIGDEQSLTQSLSTFSGNLRKISLIK 209
           DIGDEQSLTQSLSTFSG+LRKIS I+
Sbjct: 539 DIGDEQSLTQSLSTFSGHLRKISDIQ 564

[Close]

BLAST of Spg002803 vs. NCBI nr
Match: XP_038894082.1 (endonuclease MutS2 isoform X12 [Benincasa hispida])

HSP 1 Score: 365.9 bits (938), Expect = 3.2e-97
Identity = 181/203 (89.16%), Postives = 194/203 (95.57%), Query Frame = 0


Query: 6   VNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHHPL 65
           VNARASYGLSFGGTCPNL+LPEGCNS I NVCLSGD+TSEAS+ KKNEWVLYL NAHHPL
Sbjct: 196 VNARASYGLSFGGTCPNLILPEGCNSSIANVCLSGDQTSEASHSKKNEWVLYLQNAHHPL 255

Query: 66  LLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPVSV 125
           LLQQYR NLENAKRDV+NAFTEMGRKLPGGNMSWKEKEVVDIS LKMKV++LE+A PVSV
Sbjct: 256 LLQQYRENLENAKRDVQNAFTEMGRKLPGGNMSWKEKEVVDISLLKMKVEQLEKARPVSV 315

Query: 126 DFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIG 185
           DFSIS+RI+VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+FADIG
Sbjct: 316 DFSISRRIQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSIFADIG 375

Query: 186 DEQSLTQSLSTFSGNLRKISLIK 209
           DEQSLTQSLSTFSG+LRKIS I+
Sbjct: 376 DEQSLTQSLSTFSGHLRKISDIQ 398

[Close]

BLAST of Spg002803 vs. NCBI nr
Match: XP_038894089.1 (endonuclease MutS2 isoform X13 [Benincasa hispida] >XP_038894094.1 endonuclease MutS2 isoform X13 [Benincasa hispida])

HSP 1 Score: 365.9 bits (938), Expect = 3.2e-97
Identity = 181/203 (89.16%), Postives = 194/203 (95.57%), Query Frame = 0


Query: 6   VNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHHPL 65
           VNARASYGLSFGGTCPNL+LPEGCNS I NVCLSGD+TSEAS+ KKNEWVLYL NAHHPL
Sbjct: 386 VNARASYGLSFGGTCPNLILPEGCNSSIANVCLSGDQTSEASHSKKNEWVLYLQNAHHPL 445

Query: 66  LLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPVSV 125
           LLQQYR NLENAKRDV+NAFTEMGRKLPGGNMSWKEKEVVDIS LKMKV++LE+A PVSV
Sbjct: 446 LLQQYRENLENAKRDVQNAFTEMGRKLPGGNMSWKEKEVVDISLLKMKVEQLEKARPVSV 505

Query: 126 DFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIG 185
           DFSIS+RI+VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+FADIG
Sbjct: 506 DFSISRRIQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSIFADIG 565

Query: 186 DEQSLTQSLSTFSGNLRKISLIK 209
           DEQSLTQSLSTFSG+LRKIS I+
Sbjct: 566 DEQSLTQSLSTFSGHLRKISDIQ 588

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BLAST of Spg002803 vs. ExPASy Swiss-Prot
Match: P73625 (Endonuclease MutS2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.3e-21
Identity = 49/82 (59.76%), Postives = 66/82 (80.49%), Query Frame = 0


Query: 123 VSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFA 182
           V +  +I  +IRV+ ITGPNTGGKTV LKT+GL A+MAK G+++ A E+V++PWF  + A
Sbjct: 331 VPITLTIDSQIRVIAITGPNTGGKTVTLKTLGLVALMAKVGLYIPAKETVEMPWFAQILA 390

Query: 183 DIGDEQSLTQSLSTFSGNLRKI 205
           DIGDEQSL Q+LSTFSG++ +I
Sbjct: 391 DIGDEQSLQQNLSTFSGHICRI 412

[Close]

BLAST of Spg002803 vs. ExPASy Swiss-Prot
Match: Q5WEK0 (Endonuclease MutS2 OS=Bacillus clausii (strain KSM-K16) OX=66692 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.8e-19
Identity = 48/82 (58.54%), Postives = 63/82 (76.83%), Query Frame = 0


Query: 123 VSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFA 182
           V  D +I  ++R LVITGPNTGGKTV LKTIGL  +MA+SG+ V A+E  ++  F+ +FA
Sbjct: 318 VPSDMAIGDQVRSLVITGPNTGGKTVTLKTIGLLTLMAQSGLFVPAAEETELAVFEHIFA 377

Query: 183 DIGDEQSLTQSLSTFSGNLRKI 205
           DIGDEQS+ QSLSTFS +++ I
Sbjct: 378 DIGDEQSIEQSLSTFSSHMKNI 399

[Close]

BLAST of Spg002803 vs. ExPASy Swiss-Prot
Match: B8D298 (Endonuclease MutS2 OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.8e-19
Identity = 45/88 (51.14%), Postives = 63/88 (71.59%), Query Frame = 0


Query: 117 LEQAHPVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPW 176
           L +  PV +D ++    + LVITGPNTGGKTV LKT+GL  +M ++G+H+ A E   I  
Sbjct: 316 LLKVKPVPIDITVGNEFKTLVITGPNTGGKTVALKTVGLFVLMVQAGLHIPAEEETVISI 375

Query: 177 FDSVFADIGDEQSLTQSLSTFSGNLRKI 205
           F+ V+ADIGDEQS+ Q+LSTFS ++ +I
Sbjct: 376 FNGVYADIGDEQSIEQNLSTFSSHINRI 403

[Close]

BLAST of Spg002803 vs. ExPASy Swiss-Prot
Match: B9DVK7 (Endonuclease MutS2 OS=Streptococcus uberis (strain ATCC BAA-854 / 0140J) OX=218495 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.8e-19
Identity = 46/84 (54.76%), Postives = 64/84 (76.19%), Query Frame = 0


Query: 121 HPVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSV 180
           +PV+ D   +  + V+VITGPNTGGKT+ LKT+GLA +M +SG+ +LA E  +I  FD++
Sbjct: 309 NPVANDLHFADDLAVIVITGPNTGGKTIMLKTLGLAQLMGQSGLPILADEGSKIAVFDAI 368

Query: 181 FADIGDEQSLTQSLSTFSGNLRKI 205
           +ADIGDEQS+ QSLSTFS ++  I
Sbjct: 369 YADIGDEQSIEQSLSTFSSHMTHI 392

[Close]

BLAST of Spg002803 vs. ExPASy Swiss-Prot
Match: B9KYW4 (Endonuclease MutS2 OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) OX=309801 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.8e-19
Identity = 45/88 (51.14%), Postives = 66/88 (75.00%), Query Frame = 0


Query: 117 LEQAHPVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPW 176
           L++   V +D  + +R R+LVITGPNTGGKTV LKT+GL A+MA++G+ + A+    +  
Sbjct: 321 LDRRTAVPIDVELGERFRILVITGPNTGGKTVALKTVGLLALMAQAGLFIPAAPGSGLSV 380

Query: 177 FDSVFADIGDEQSLTQSLSTFSGNLRKI 205
           F ++F DIGDEQS+ Q+LSTFS ++R+I
Sbjct: 381 FPAIFVDIGDEQSIEQNLSTFSSHMRRI 408

[Close]

BLAST of Spg002803 vs. ExPASy TrEMBL
Match: A0A5D3BZK6 (Endonuclease MutS2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold351G00060 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 8.0e-94
Identity = 176/203 (86.70%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 6   VNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHHPL 65
           VNARASYGLSFGGTCPNL+L EGCNS I NVCLSGD+ SEAS+ KKNEWVLYL N HHPL
Sbjct: 341 VNARASYGLSFGGTCPNLILLEGCNSSIANVCLSGDQ-SEASHSKKNEWVLYLQNTHHPL 400

Query: 66  LLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPVSV 125
           LLQQYR NLENAKRDV+NAF E+GRKLPGGNMSWKEKEVVDIS  KMKV++LEQAHPVSV
Sbjct: 401 LLQQYRENLENAKRDVKNAFNEVGRKLPGGNMSWKEKEVVDISLFKMKVEQLEQAHPVSV 460

Query: 126 DFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIG 185
           DFSIS+R++VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+FADIG
Sbjct: 461 DFSISRRVQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSIFADIG 520

Query: 186 DEQSLTQSLSTFSGNLRKISLIK 209
           DEQSLTQSLSTFSG+LRKIS I+
Sbjct: 521 DEQSLTQSLSTFSGHLRKISDIQ 542

[Close]

BLAST of Spg002803 vs. ExPASy TrEMBL
Match: A0A1S3CJJ8 (endonuclease MutS2 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 8.0e-94
Identity = 176/203 (86.70%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 6   VNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHHPL 65
           VNARASYGLSFGGTCPNL+L EGCNS I NVCLSGD+ SEAS+ KKNEWVLYL N HHPL
Sbjct: 257 VNARASYGLSFGGTCPNLILLEGCNSSIANVCLSGDQ-SEASHSKKNEWVLYLQNTHHPL 316

Query: 66  LLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPVSV 125
           LLQQYR NLENAKRDV+NAF E+GRKLPGGNMSWKEKEVVDIS  KMKV++LEQAHPVSV
Sbjct: 317 LLQQYRENLENAKRDVKNAFNEVGRKLPGGNMSWKEKEVVDISLFKMKVEQLEQAHPVSV 376

Query: 126 DFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIG 185
           DFSIS+R++VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+FADIG
Sbjct: 377 DFSISRRVQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSIFADIG 436

Query: 186 DEQSLTQSLSTFSGNLRKISLIK 209
           DEQSLTQSLSTFSG+LRKIS I+
Sbjct: 437 DEQSLTQSLSTFSGHLRKISDIQ 458

[Close]

BLAST of Spg002803 vs. ExPASy TrEMBL
Match: A0A1S3CHZ5 (endonuclease MutS2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 8.0e-94
Identity = 176/203 (86.70%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 6   VNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHHPL 65
           VNARASYGLSFGGTCPNL+L EGCNS I NVCLSGD+ SEAS+ KKNEWVLYL N HHPL
Sbjct: 341 VNARASYGLSFGGTCPNLILLEGCNSSIANVCLSGDQ-SEASHSKKNEWVLYLQNTHHPL 400

Query: 66  LLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPVSV 125
           LLQQYR NLENAKRDV+NAF E+GRKLPGGNMSWKEKEVVDIS  KMKV++LEQAHPVSV
Sbjct: 401 LLQQYRENLENAKRDVKNAFNEVGRKLPGGNMSWKEKEVVDISLFKMKVEQLEQAHPVSV 460

Query: 126 DFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIG 185
           DFSIS+R++VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASES QIPWFDS+FADIG
Sbjct: 461 DFSISRRVQVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESAQIPWFDSIFADIG 520

Query: 186 DEQSLTQSLSTFSGNLRKISLIK 209
           DEQSLTQSLSTFSG+LRKIS I+
Sbjct: 521 DEQSLTQSLSTFSGHLRKISDIQ 542

[Close]

BLAST of Spg002803 vs. ExPASy TrEMBL
Match: A0A0A0KVU2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G119250 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 1.1e-93
Identity = 176/202 (87.13%), Postives = 189/202 (93.56%), Query Frame = 0


Query: 6   VNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHHPL 65
           VNARASYGLSFGGTCPNLVL EGCNS I NVCLSGD+ SEAS+LKKNEWVLYL N HHPL
Sbjct: 341 VNARASYGLSFGGTCPNLVLLEGCNSSIANVCLSGDQ-SEASHLKKNEWVLYLQNTHHPL 400

Query: 66  LLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPVSV 125
           LLQQYR NL+NAKRDV+NAF EMGRK PGGNMSWKEKEV+DIS  KMKV +LEQA PVSV
Sbjct: 401 LLQQYRENLKNAKRDVKNAFNEMGRKPPGGNMSWKEKEVIDISLFKMKVDQLEQARPVSV 460

Query: 126 DFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIG 185
           DFSIS+RI+VLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASESVQIPWFDS+FADIG
Sbjct: 461 DFSISRRIKVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESVQIPWFDSIFADIG 520

Query: 186 DEQSLTQSLSTFSGNLRKISLI 208
           DEQSLTQSLSTFSG+LRKIS++
Sbjct: 521 DEQSLTQSLSTFSGHLRKISIV 541

[Close]

BLAST of Spg002803 vs. ExPASy TrEMBL
Match: A0A6J1JC76 (uncharacterized protein LOC111483140 isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111483140 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.8e-93
Identity = 176/208 (84.62%), Postives = 190/208 (91.35%), Query Frame = 0


Query: 1   MANQKVNARASYGLSFGGTCPNLVLPEGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPN 60
           M  +KVNARASYGLSFGG CPNL+LP GCNS I NV LSGD+ SEAS+ K+N+WVLYLPN
Sbjct: 318 MLTEKVNARASYGLSFGGACPNLILPGGCNSSIANVYLSGDQISEASHPKENKWVLYLPN 377

Query: 61  AHHPLLLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQA 120
           AHHPLL QQYR +LENAKRDVRNA TE+GRKLPGGNMSWKEKEV DIS LKMKV++LEQA
Sbjct: 378 AHHPLLTQQYRESLENAKRDVRNAVTEIGRKLPGGNMSWKEKEVADISLLKMKVEQLEQA 437

Query: 121 HPVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSV 180
            PVSVDF+IS RIRVLVITGPNTGGKTVCLKTIGLAAMMAKSG+HVLASESVQIPWFDSV
Sbjct: 438 RPVSVDFAISHRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGLHVLASESVQIPWFDSV 497

Query: 181 FADIGDEQSLTQSLSTFSGNLRKISLIK 209
            ADIGDEQSLTQSLSTFSG+LRKIS I+
Sbjct: 498 LADIGDEQSLTQSLSTFSGHLRKISEIQ 525

[Close]

BLAST of Spg002803 vs. TAIR 10
Match: AT5G54090.1 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-41
Identity = 93/204 (45.59%), Postives = 122/204 (59.80%), Query Frame = 0


Query: 6   VNARASYGLSFGGTCPNLVLP--EGCNSCITNVCLSGDRTSEASNLKKNEWVLYLPNAHH 65
           +NARA+Y  ++GG  P++ LP  +   S                 L K EW+LYLP  +H
Sbjct: 331 INARATYSRAYGGAHPDIYLPPEDEVESLSAGENSPDINLPSEKPLSKKEWLLYLPRCYH 390

Query: 66  PLLLQQYRVNLENAKRDVRNAFTEMGRKLPGGNMSWKEKEVVDISFLKMKVQKLEQAHPV 125
           PLLL Q++  +   +  V+                          F K     L  A P+
Sbjct: 391 PLLLYQHKKGIRKTRETVK--------------------------FHKTADTVLSGAPPI 450

Query: 126 SVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFAD 185
             DF IS+  RVLVITGPNTGGKT+CLK++GLAAMMAKSG++VLA+ES +IPWFD+++AD
Sbjct: 451 PADFQISKGTRVLVITGPNTGGKTICLKSVGLAAMMAKSGLYVLATESARIPWFDNIYAD 508

Query: 186 IGDEQSLTQSLSTFSGNLRKISLI 208
           IGDEQSL QSLSTFSG+L++IS I
Sbjct: 511 IGDEQSLLQSLSTFSGHLKQISEI 508

[Close]

BLAST of Spg002803 vs. TAIR 10
Match: AT1G65070.1 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 100.1 bits (248), Expect = 3.1e-21
Identity = 47/86 (54.65%), Postives = 64/86 (74.42%), Query Frame = 0


Query: 122 PVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVF 181
           PV VD  +    +V+VI+GPNTGGKT  LKT+GL ++M+KSG+++ A    ++PWFD + 
Sbjct: 384 PVPVDIKVESSAKVVVISGPNTGGKTALLKTLGLLSLMSKSGMYLPAKNCPRLPWFDLIL 443

Query: 182 ADIGDEQSLTQSLSTFSGNLRKISLI 208
           ADIGD QSL QSLSTFSG++ +I  I
Sbjct: 444 ADIGDPQSLEQSLSTFSGHISRIRQI 469

[Close]

BLAST of Spg002803 vs. TAIR 10
Match: AT1G65070.2 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 100.1 bits (248), Expect = 3.1e-21
Identity = 47/86 (54.65%), Postives = 64/86 (74.42%), Query Frame = 0


Query: 122 PVSVDFSISQRIRVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVF 181
           PV VD  +    +V+VI+GPNTGGKT  LKT+GL ++M+KSG+++ A    ++PWFD + 
Sbjct: 384 PVPVDIKVESSAKVVVISGPNTGGKTALLKTLGLLSLMSKSGMYLPAKNCPRLPWFDLIL 443

Query: 182 ADIGDEQSLTQSLSTFSGNLRKISLI 208
           ADIGD QSL QSLSTFSG++ +I  I
Sbjct: 444 ADIGDPQSLEQSLSTFSGHISRIRQI 469

[Close]

BLAST of Spg002803 vs. TAIR 10
Match: AT3G24320.1 (MUTL protein homolog 1 )

HSP 1 Score: 43.1 bits (100), Expect = 4.5e-04
Identity = 27/73 (36.99%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 135 VLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIGDEQSLTQSL 194
           + ++TGPN GGK+  L++I  AA++  SG+ V A ES  IP FDS+   +    S     
Sbjct: 763 LFLLTGPNGGGKSSLLRSICAAALLGISGLMVPA-ESACIPHFDSIMLHMKSYDSPVDGK 822

Query: 195 STFSGNLRKISLI 208
           S+F   + +I  I
Sbjct: 823 SSFQVEMSEIRSI 834

[Close]

BLAST of Spg002803 vs. TAIR 10
Match: AT3G24495.1 (MUTS homolog 7 )

HSP 1 Score: 42.7 bits (99), Expect = 5.8e-04
Identity = 24/64 (37.50%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 134 RVLVITGPNTGGKTVCLKTIGLAAMMAKSGIHVLASESVQIPWFDSVFADIGDEQSLTQS 193
           R L++TGPN GGK+  L+   LA + A+ G +V   ES +I   D++F  +G    +   
Sbjct: 847 RSLLLTGPNMGGKSTLLRATCLAVIFAQLGCYV-PCESCEISLVDTIFTRLGASDRIMTG 906

Query: 194 LSTF 198
            STF
Sbjct: 907 ESTF 909

The following BLAST results are available for this feature:

BLAST of Spg002803 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894074.1	1.3e-98	87.98	endonuclease MutS2 isoform X11 [Benincasa hispida]	[more]
XP_038893999.1	5.0e-98	88.35	endonuclease MutS2 isoform X5 [Benincasa hispida]	[more]
XP_038894006.1	5.0e-98	88.35	endonuclease MutS2 isoform X6 [Benincasa hispida]	[more]
XP_038894082.1	3.2e-97	89.16	endonuclease MutS2 isoform X12 [Benincasa hispida]	[more]
XP_038894089.1	3.2e-97	89.16	endonuclease MutS2 isoform X13 [Benincasa hispida] >XP_038894094.1 endonuclease ...	[more]

BLAST of Spg002803 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P73625	1.3e-21	59.76	Endonuclease MutS2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN...	[more]
Q5WEK0	2.8e-19	58.54	Endonuclease MutS2 OS=Bacillus clausii (strain KSM-K16) OX=66692 GN=mutS2 PE=3 S...	[more]
B8D298	2.8e-19	51.14	Endonuclease MutS2 OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)...	[more]
B9DVK7	4.8e-19	54.76	Endonuclease MutS2 OS=Streptococcus uberis (strain ATCC BAA-854 / 0140J) OX=2184...	[more]
B9KYW4	4.8e-19	51.14	Endonuclease MutS2 OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2...	[more]

BLAST of Spg002803 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BZK6	8.0e-94	86.70	Endonuclease MutS2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CJJ8	8.0e-94	86.70	endonuclease MutS2 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1	[more]
A0A1S3CHZ5	8.0e-94	86.70	endonuclease MutS2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1	[more]
A0A0A0KVU2	1.1e-93	87.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G119250 PE=4 SV=1	[more]
A0A6J1JC76	1.8e-93	84.62	uncharacterized protein LOC111483140 isoform X5 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Spg002803 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54090.1	1.2e-41	45.59	DNA mismatch repair protein MutS, type 2	[more]
AT1G65070.1	3.1e-21	54.65	DNA mismatch repair protein MutS, type 2	[more]
AT1G65070.2	3.1e-21	54.65	DNA mismatch repair protein MutS, type 2	[more]
AT3G24320.1	4.5e-04	36.99	MUTL protein homolog 1	[more]
AT3G24495.1	5.8e-04	37.50	MUTS homolog 7	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 67..87
None	No IPR available	PANTHER	PTHR11361:SF132	DNA MISMATCH REPAIR PROTEIN MUTS, TYPE 2	coord: 5..208
IPR000432	DNA mismatch repair protein MutS, C-terminal	SMART	SM00534	mutATP5	coord: 133..273 e-value: 1.1E-6 score: -28.8
IPR000432	DNA mismatch repair protein MutS, C-terminal	PFAM	PF00488	MutS_V	coord: 136..206 e-value: 9.4E-12 score: 45.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 96..209 e-value: 6.6E-30 score: 106.1
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 121..208
IPR045076	DNA mismatch repair MutS family	PANTHER	PTHR11361	DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER	coord: 5..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002803.1	Spg002803.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006298	mismatch repair
biological_process	GO:0045910	negative regulation of DNA recombination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0030983	mismatched DNA binding