Spg002775 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002775
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	beta-carotene hydroxylase 2, chloroplastic-like
Location	scaffold6: 1189443 .. 1192333 (+)
RNA-Seq Expression	Spg002775
Synteny	Spg002775

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTAACTTCCTCCAATCTCTCTCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCCTCCGGAGCAGTAGAATCCAATGGAAGATGCGGAGAAAAACGCTGCTCACCGTCTGTGTGCTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCAATGAAGGATCGCCGATTGTAGTCCCTCAGATCCCGTCGCCTCATGTTGCAGAGAAATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCCGCGGTGATGTCTAGTTTTGGAATTACTTCCATGGCTATCATGGCTGTTTACTACCGATTTTACTGGCAAATGGAGGTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGAAACTTGAATTGTTTTGTTTTTTTTTTTTCATTGGCGCCGTATTCTTCTGACTGAATATTAAATTGCGATTGTTTAATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCAGAAATGTTCGGTACATTTTCTCTATCCGTTGGTGCCGCTGTAAGTTTTCAATTTCCTTTAATCTCTCTATTCTCTACTTTTCTCGAAGAATTCGATTTGCTATAAGCTGATTTTTCACAATCAATTTGGGATTATCAGGTCGGCATGGAGTTTTGGGCAAGATGGGCGCATAGGGCACTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTATAGGTTTCTAATCCTTCTTCTCTATAGAATTTCGTTTGTGTTCGTCTGCTGAGAAAAATTCAATCAACAATAATCCACCGCGAAACTTAATTTCATAGCAAACAAAACAGTATTCAAATGGAAAAAAGAATCGTTGACGCTTGGACTTGAAGATTGCCGACCAATCGCACCATTCCTAAACTAATCCTCTTCTTTAGAATTTTCTTTGTCTGGCTGATGATTAAATTCAATTAACAATCGTCCGCTGTGGACCTTTCTCTCATTTTCCATGTTCACTTTCGTAGCAAACAAACAACACCGAATTAAAAAAGAAAATTCGTTGACGCTTGGACTTTGTTGATCTTGATTATTACAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTTCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCTATCACTTTCTCTCTCATAATCTTTTAGAATCTCAGTTTGTTCATATTCCAACTGTTTAATGGTTATTTAATCACTGGGGATTAGCGATAATGACGGTATAAAATGACGAAAATAGCCCTGAAAAAATTTGGTAATTGAGAAAGTTTTGACACGCTGGCGGGCTGTGGCAGGGCCTTGGAATTACAGTTTTCGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCCGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTTGCTGCTGCTCACCAGGTAAATTCAATTTCTAAATATTAATTTCTTTTTTTTTTTTTTTAATGCTTATTGCTTAATCTTTAAAGAAAAGCAAATTTGTTATTATTTAAATCTTATTTTGATCTCAAAATTTTGATACTGTCTCTCTTTACTCTCCATTGTGTTGACTTTTCATTAATGATTCTAACAAAAACTCAATTATGTTTCAAACCTTAATATTTGGATCACTAGATGTACTTGTAAGATGCTCATGTAGATTTATAATTAAAAAAAAAGATGCTCATGTAGATTTCTACTAATAGAATATACGGAAAAAATGGGGTTTTTAATGTCTTTTTTTGTTCTTAGAATATTGAGAAACGTATGGATCTCGATCGAAGAATATAAAAACTTAGGTCTATCAAATGAGTCTTATAAATAAAAATATGATTTAAAATCAATCGACTAATAATATATCAGAAATAATTGAAATAAGAAAATAATGAAAATATAATAATATGCTTAGACTTCGTGCAATTTTTAATAAACTCATATTAGATTATGTTTTTTTACGGTTAAAATTAAAAACGTGTTATTAACTTGCATATAGAAAAGTAATTACACGTGTCTCAAGTGTAAATCTAAATTTAAAACCATTTAAATATTAAAGCTTATGATTTTTTTTTCTTCTTCTTGGCTTTAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTTAGTTTCGGGTCTACTTATCTTCTTTTTTTTTTTTTTTAATCTTAAAATTTAAATTTATTATTTTCAAAAATTTTAATAAATGGCTTTCCCAAAAATGGTGTGACGCAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCTTGATTAACCAGTCAATCGAGGGTCCCACTATTAATATTATGTGAAAATGAGAAGTAAAAAAAGAAAAAGCCCTCCCCAAGCAGTAACTTTTCTCTTTTTTGATATTGAAAAGTGAATGTTATTTAGATCAGATCACAGCCTTTTTTTCCCTTTTTACTGTATGAGGTAAAAGGGTAGAAGAATGAGATGGTAAATTTGGTAGAAAGGATTTGCTTTTATTAGGACCTCCCTCTACTTTTTCGGTCTATGTGACTGTAAAGAAAAATAAAGTCGGCCGAAGGTAAAATAACACACTCATAATTTTGTGTAATTTTCGATATTTCATAATTTAAGCAAGTAAAATGTCAAATACATATATAGAGTTCATACTTTTGAGTTTTGAAAGGGCAAAACTTATTGGGAATCGTGTAATTG

mRNA sequence

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTAACTTCCTCCAATCTCTCTCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCCTCCGGAGCAGTAGAATCCAATGGAAGATGCGGAGAAAAACGCTGCTCACCGTCTGTGTGCTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCAATGAAGGATCGCCGATTGTAGTCCCTCAGATCCCGTCGCCTCATGGCGGAGAGATTCCTTTCTCAGAAATGTTCGGTACATTTTCTCTATCCGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCAAGATGGGCGCATAGGGCACTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTTCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACAGTTTTCGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCCGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCTTGA

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTAACTTCCTCCAATCTCTCTCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCCTCCGGAGCAGTAGAATCCAATGGAAGATGCGGAGAAAAACGCTGCTCACCGTCTGTGTGCTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCAATGAAGGATCGCCGATTGTAGTCCCTCAGATCCCGTCGCCTCATGGCGGAGAGATTCCTTTCTCAGAAATGTTCGGTACATTTTCTCTATCCGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCAAGATGGGCGCATAGGGCACTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTTCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACAGTTTTCGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCCGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCTTGA

Protein sequence

MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVEDQNSSGEVENLANEGSPIVVPQIPSPHGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKSTATKSINGS

Homology

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BLAST of Spg002775 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 494.6 bits (1272), Expect = 5.4e-136
Identity = 250/310 (80.65%), Postives = 258/310 (83.23%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSAAL PKPLHL LTSS+LSPKPR PFLFPPSV R+SRIQWKMRR+TL TVCVLVE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQNSSGEVENL++E SPI+VPQIPSPH                                 
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

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BLAST of Spg002775 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 494.2 bits (1271), Expect = 7.1e-136
Identity = 249/310 (80.32%), Postives = 257/310 (82.90%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSA LGPKP H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQ+SS EVENLA+E  PI+VPQIPSPH                                 
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

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BLAST of Spg002775 vs. NCBI nr
Match: XP_023541483.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 493.4 bits (1269), Expect = 1.2e-135
Identity = 248/310 (80.00%), Postives = 256/310 (82.58%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAA +SA LGPKP H+FLTSSNLSPKPRTPFL+P SV RSSRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAASVSAVLGPKPFHVFLTSSNLSPKPRTPFLYPSSVFRSSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQ+SS EVENLA+E  PI+VPQIPSPH                                 
Sbjct: 61  DQSSSSEVENLADENLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

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BLAST of Spg002775 vs. NCBI nr
Match: XP_022944865.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 493.0 bits (1268), Expect = 1.6e-135
Identity = 248/310 (80.00%), Postives = 256/310 (82.58%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSA LGPKP H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQ+SS EVENLA+E  PI+VPQIPSPH                                 
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGGL+ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

[Close]

BLAST of Spg002775 vs. NCBI nr
Match: KAG6574199.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.1 bits (1263), Expect = 6.0e-135
Identity = 248/310 (80.00%), Postives = 256/310 (82.58%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSA LGPK  H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKSFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQ+SS EVENLA+E  PI+VPQIPSPH                                 
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

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BLAST of Spg002775 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 346.3 bits (887), Expect = 3.1e-94
Identity = 162/180 (90.00%), Postives = 170/180 (94.44%), Query Frame = 0


Query: 88  GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVF 147
           GGE+PFSEMFGTF+LSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREGPFELNDVF
Sbjct: 134 GGEVPFSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVF 193

Query: 148 AIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANV 207
           AIINAVPAIALL YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANV
Sbjct: 194 AIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANV 253

Query: 208 PYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKSTATKSINGS 267
           PY RKVAAAH LHHS+KFNGVPYGLFLGPKELEEVGGLEELEKE+NRR     T+ I GS
Sbjct: 254 PYLRKVAAAHSLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRR-----TRYIKGS 308

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BLAST of Spg002775 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.3e-92
Identity = 188/299 (62.88%), Postives = 208/299 (69.57%), Query Frame = 0


Query: 18  LTSSNLSPK-----PRTPFL--FPPSVLRSSRIQWKMRRKTLLTVCVLVE---------- 77
           ++SS+L+PK       TP L  FPP  L S  +  + R K  LTVC ++E          
Sbjct: 21  ISSSSLTPKSSPVSTSTPTLVVFPPFKLVSKSL--RTRSKPRLTVCFVLEEKELRGKLVV 80

Query: 78  ---DQNSSGEV------------ENLA-------NEGSPIVVPQIPSP------------ 137
              D + +GEV            E LA       +E    +V  + S             
Sbjct: 81  ASDDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVY 140

Query: 138 -------HGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREG 197
                   GGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWHASLWHMHESHHKPREG
Sbjct: 141 YRFSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREG 200

Query: 198 PFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRF 257
           PFELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF
Sbjct: 201 PFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRF 260

Query: 258 PVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKS 259
           PVGPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPKELEEVGGL+ LE EINRR K+
Sbjct: 261 PVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRTKN 317

[Close]

BLAST of Spg002775 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 4.2e-91
Identity = 154/172 (89.53%), Postives = 168/172 (97.67%), Query Frame = 0


Query: 88  GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVF 147
           GGE+PFSEMF TF+L+ GAA+GME+WARWAHRALWHASLWHMHESHH+PREGPFELND+F
Sbjct: 141 GGEMPFSEMFCTFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIF 200

Query: 148 AIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANV 207
           AIINAVPAIALLS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANV
Sbjct: 201 AIINAVPAIALLSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANV 260

Query: 208 PYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKST 260
           PYF++VAAAHQLHHSDKF+GVPYGLFLGPKELEEVG LEELEKE+NRRIKS+
Sbjct: 261 PYFQRVAAAHQLHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIKSS 312

[Close]

BLAST of Spg002775 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 7.2e-91
Identity = 188/317 (59.31%), Postives = 212/317 (66.88%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFL---FPPSVLRSSRIQWKMRRKTLLTVCV 60
           MAAGLS A+  KPLH   +SS+   + R P     F PS LR  R           +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPS-LRFKR----------FSVCY 60

Query: 61  LVEDQNSSGEVEN----------------------------LANEGSPIVVPQIPSP--- 120
           +VE++  +  +EN                              +E S  ++  + S    
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 ----------------HGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMH 180
                            GGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEK 268
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

[Close]

BLAST of Spg002775 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 7.5e-88
Identity = 179/302 (59.27%), Postives = 204/302 (67.55%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLV 60
           MAAGLS  A+  KPL+    S+N    P +  +FPPS+  +       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSG-------------------------EVENLANEGSPIVVPQIPSP-------- 120
           E++  S                          + E   +E    ++  + S         
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 -----------HGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
                       GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 258
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

[Close]

BLAST of Spg002775 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 7.6e-136
Identity = 248/310 (80.00%), Postives = 256/310 (82.58%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSA LGPKP H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQ+SS EVENLA+E  PI+VPQIPSPH                                 
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGGL+ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

[Close]

BLAST of Spg002775 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.1e-134
Identity = 247/310 (79.68%), Postives = 257/310 (82.90%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAAGLSAA  PKP HLFLTSS+LSPKP +PFLFPPSV R+SR QWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
            QNSSG+V+NL++E SPIVVPQIPSPH                                 
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

[Close]

BLAST of Spg002775 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.2e-134
Identity = 248/310 (80.00%), Postives = 255/310 (82.26%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAA LSAALGPKPLHLFL SS+LS KPRTPFLFPPSV R+SRIQ KMRRKTL TVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQNSSGEVENL++E  PIVVPQIPSP+                                 
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWH+S+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

[Close]

BLAST of Spg002775 vs. ExPASy TrEMBL
Match: A0A6J1HX82 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 246/310 (79.35%), Postives = 253/310 (81.61%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAA LSA LGPKP H+FLTSSNLSPKPRTP LFP SV R+SRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQ+SS EVENLA+E   I+VPQIPSPH                                 
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

[Close]

BLAST of Spg002775 vs. ExPASy TrEMBL
Match: G1FQR0 (Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 246/310 (79.35%), Postives = 253/310 (81.61%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLVE 60
           MAA LSA LGPKP H+FLTSSNLSPKPRTP LFP SV R+SRIQWKMRRKTLLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPH--------------------------------- 120
           DQ+SS EVENLA+E   I+VPQIPSPH                                 
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 ----------GGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
                     GGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 268
           RFPVGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

[Close]

BLAST of Spg002775 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 335.1 bits (858), Expect = 5.1e-92
Identity = 188/317 (59.31%), Postives = 212/317 (66.88%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFL---FPPSVLRSSRIQWKMRRKTLLTVCV 60
           MAAGLS A+  KPLH   +SS+   + R P     F PS LR  R           +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPS-LRFKR----------FSVCY 60

Query: 61  LVEDQNSSGEVEN----------------------------LANEGSPIVVPQIPSP--- 120
           +VE++  +  +EN                              +E S  ++  + S    
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 ----------------HGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMH 180
                            GGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEK 268
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

[Close]

BLAST of Spg002775 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 325.1 bits (832), Expect = 5.3e-89
Identity = 179/302 (59.27%), Postives = 204/302 (67.55%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLV 60
           MAAGLS  A+  KPL+    S+N    P +  +FPPS+  +       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSG-------------------------EVENLANEGSPIVVPQIPSP-------- 120
           E++  S                          + E   +E    ++  + S         
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 -----------HGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
                       GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 258
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

[Close]

BLAST of Spg002775 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 177.2 bits (448), Expect = 1.8e-44
Identity = 110/226 (48.67%), Postives = 128/226 (56.64%), Query Frame = 0


Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFL---FPPSVLRSSRIQWKMRRKTLLTVCV 60
           MAAGLS A+  KPLH   +SS+   + R P     F PS LR  R           +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPS-LRFKR----------FSVCY 60

Query: 61  LVEDQNSSGEVEN----------------------------LANEGSPIVVPQIPSP--- 120
           +VE++  +  +EN                              +E S  ++  + S    
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 ----------------HGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMH 177
                            GGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

[Close]

BLAST of Spg002775 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 171.8 bits (434), Expect = 7.5e-43
Identity = 117/262 (44.66%), Postives = 150/262 (57.25%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKTLLTVCVLV 60
           MAAGLS  A+  KPL+    S+N    P +  +FPPS+  +       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVEN----LANEGSPIVVPQIPSPHGGEIPFSEMFGTFSLSVGAAVGMEFWAR 120
           E++  S  +++     +   S  ++         E   SE F     +V ++ G+     
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGI---TS 120

Query: 121 WAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFG 180
            A  A+++   W M       + G   + ++F           LS G             
Sbjct: 121 MAIMAVYYRFSWQM-------KGGEVSVLEMFGTF-------ALSVG------------A 180

Query: 181 AGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLG 240
           AGLGIT+FGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLG
Sbjct: 181 AGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLG 225

Query: 241 PKELEEVGGLEELEKEINRRIK 258
           PKE+EEVGG EELEKEI+RRIK
Sbjct: 241 PKEVEEVGGKEELEKEISRRIK 225

The following BLAST results are available for this feature:

BLAST of Spg002775 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877448.1	5.4e-136	80.65	beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida]	[more]
KAG7013258.1	7.1e-136	80.32	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_023541483.1	1.2e-135	80.00	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp...	[more]
XP_022944865.1	1.6e-135	80.00	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
KAG6574199.1	6.0e-135	80.00	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]

BLAST of Spg002775 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	3.1e-94	90.00	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	1.3e-92	62.88	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
O49815	4.2e-91	89.53	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9SZZ8	7.2e-91	59.31	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9LTG0	7.5e-88	59.27	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Spg002775 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ94	7.6e-136	80.00	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata...	[more]
A0A0A0L606	1.1e-134	79.68	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1J1S8	4.2e-134	80.00	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1HX82	1.6e-133	79.35	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima O...	[more]
G1FQR0	1.6e-133	79.35	Chloroplast beta-carotene hydroxylase OS=Cucurbita moschata OX=3662 GN=CHYB PE=2...	[more]

BLAST of Spg002775 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	5.1e-92	59.31	beta-hydroxylase 1	[more]
AT5G52570.1	5.3e-89	59.27	beta-carotene hydroxylase 2	[more]
AT4G25700.2	1.8e-44	48.67	beta-hydroxylase 1	[more]
AT5G52570.2	7.5e-43	44.66	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 106..233 e-value: 6.4E-14 score: 52.4
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 87..258
None	No IPR available	PANTHER	PTHR31899:SF10	BETA-CAROTENE HYDROXYLASE	coord: 87..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002775.1	Spg002775.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity