Spg002716 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002716
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionFe2OG dioxygenase domain-containing protein
Locationscaffold6: 2992291 .. 2994114 (-)
RNA-Seq ExpressionSpg002716
SyntenySpg002716
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCTAAAAGGTGAGAGTTCAGGCTCATTTCCCACAGGAAAGACTGCACAAGAGAAAGGCTTTAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACACCGCCCGAGTCTATCCCCGGAAACTGCCATTGTTCCTGTCATTGACATGGCTTCTCTAAGATCAACTGATTCAGCTCAAAGGGCCCTTGCCATTGAAGAGCTCAGAAAAGCTTGCATTAGCCTAGGCTTCTTTCAGGTAAGTTTCAAGCAACCTATACCTACCTACCTCCTTTAGCATTGTTAGTACTTCTAGTTCAATTCTTCATATTGATCCAGGAAACTGTAAAGGTTTATATATTAGCTCTTAACTTCTATCTTTTTTCTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCGCTCGGCCAAGCCTATGAGTTCTTCGACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTTGCAGAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTTGATAAAATAAAATTTTGGAGAGTCTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGATCGACTACTGGCCCACCAGTCCACCTAGCTATAGGTAATTTAATATTTTAATTCTCCATCTCCACTTCCTAACTAATTTTCATTCTTTTTCTTTTCCTGATTACCATGCAAAATTGACTACACGAGAAGGCTGTACAATGAAATTCAACAAAAGTATACAATGAAACAATTGAACCGCCAAAAGAGAGCACCGCTATGCATATGCTGCCGCCTTTGAACCATAATTCCAGCTAACAATGATATTTTTATACATGCTAACGTTTAGTACGGTCTAAAAGTTTGGATTTTTCTATGCATACTCAGGGTACTATTAAGGCATTCTACTTCAATGAACCATAAATATAGGAGTTGGAACGCTCACTCAATAAATAACAATCAAAACATGATAATTTATAAAAGCACTTCTTACTTGCAGACAAGAGTTGACGTCGATACGGACAAAAAATTTGACGTCTCTGATGAAAATATCAATATCAATGAATATTATAATACCCCCTTCAAAAAAAAAAAAGTTATTATGTAATCTTTTGCTCTGGGAAAACCACATATCAATTGAAATTGTACTTCAAACAAACCAAGATCCTAAATGCATAGAGATGTGTCCTTGAGATTATATGTATACACACACACACATTATATATATGTACAACCGTTTCAGGGAAAAGATGGGGCAATACACCAAGGAGGTGAGAAAAGTGAGCCTAGAGGTAATGGAAGCCATAACTGAAAGCTTGGGTTTAAGTCCAACATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGACAGTAAACTGCTACCCTCCATGTCCACAGCCAGAGGTCGCCCTTGGGTTGCCACCACATACAGACTACAGTTGCATAACCACAGTTCTCCAAAGCAGCCAGGGCCTTGAAATCATGGACACGGCTGATGGGACATGGAAGCTGGTTCCAAAGATTGATGGCGCTCTGCAAGTCCATGTGGGCGATCATGTGGAAGTTCTGAGCAATGGCCTTTACAAGGGTGTCGTCCACAGAGCCACTGTCAATTCTGAGAGCACAAGAATCTCCATTACCAGTCTGCACAGCTTGGCAATGGACGAGAAAATGAAGCCTGCTGAAGAGCTTGTGGATGAACAGAATCCAAAAAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAATGATCTTGCAGAAGGAAAGAACTTCATCGGCACACTCAAGATCCAGGAGGAATAA

mRNA sequence

ATGGGGCTAAAAGGTGAGAGTTCAGGCTCATTTCCCACAGGAAAGACTGCACAAGAGAAAGGCTTTAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACACCGCCCGAGTCTATCCCCGGAAACTGCCATTGTTCCTGTCATTGACATGGCTTCTCTAAGATCAACTGATTCAGCTCAAAGGGCCCTTGCCATTGAAGAGCTCAGAAAAGCTTGCATTAGCCTAGGCTTCTTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCGCTCGGCCAAGCCTATGAGTTCTTCGACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTTGCAGAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTTGATAAAATAAAATTTTGGAGAGTCTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGATCGACTACTGGCCCACCAGTCCACCTAGCTATAGGGAAAAGATGGGGCAATACACCAAGGAGGTGAGAAAAGTGAGCCTAGAGGTAATGGAAGCCATAACTGAAAGCTTGGGTTTAAGTCCAACATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGACAGTAAACTGCTACCCTCCATGTCCACAGCCAGAGGTCGCCCTTGGGTTGCCACCACATACAGACTACAGTTGCATAACCACAGTTCTCCAAAGCAGCCAGGGCCTTGAAATCATGGACACGGCTGATGGGACATGGAAGCTGGTTCCAAAGATTGATGGCGCTCTGCAAGTCCATGTGGGCGATCATGTGGAAGTTCTGAGCAATGGCCTTTACAAGGGTGTCGTCCACAGAGCCACTGTCAATTCTGAGAGCACAAGAATCTCCATTACCAGTCTGCACAGCTTGGCAATGGACGAGAAAATGAAGCCTGCTGAAGAGCTTGTGGATGAACAGAATCCAAAAAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAATGATCTTGCAGAAGGAAAGAACTTCATCGGCACACTCAAGATCCAGGAGGAATAA

Coding sequence (CDS)

ATGGGGCTAAAAGGTGAGAGTTCAGGCTCATTTCCCACAGGAAAGACTGCACAAGAGAAAGGCTTTAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACACCGCCCGAGTCTATCCCCGGAAACTGCCATTGTTCCTGTCATTGACATGGCTTCTCTAAGATCAACTGATTCAGCTCAAAGGGCCCTTGCCATTGAAGAGCTCAGAAAAGCTTGCATTAGCCTAGGCTTCTTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCGCTCGGCCAAGCCTATGAGTTCTTCGACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTTGCAGAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTTGATAAAATAAAATTTTGGAGAGTCTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGATCGACTACTGGCCCACCAGTCCACCTAGCTATAGGGAAAAGATGGGGCAATACACCAAGGAGGTGAGAAAAGTGAGCCTAGAGGTAATGGAAGCCATAACTGAAAGCTTGGGTTTAAGTCCAACATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGACAGTAAACTGCTACCCTCCATGTCCACAGCCAGAGGTCGCCCTTGGGTTGCCACCACATACAGACTACAGTTGCATAACCACAGTTCTCCAAAGCAGCCAGGGCCTTGAAATCATGGACACGGCTGATGGGACATGGAAGCTGGTTCCAAAGATTGATGGCGCTCTGCAAGTCCATGTGGGCGATCATGTGGAAGTTCTGAGCAATGGCCTTTACAAGGGTGTCGTCCACAGAGCCACTGTCAATTCTGAGAGCACAAGAATCTCCATTACCAGTCTGCACAGCTTGGCAATGGACGAGAAAATGAAGCCTGCTGAAGAGCTTGTGGATGAACAGAATCCAAAAAAGTATAAAGAGAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAATGATCTTGCAGAAGGAAAGAACTTCATCGGCACACTCAAGATCCAGGAGGAATAA

Protein sequence

MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSAQRALAIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKIQEE
Homology
BLAST of Spg002716 vs. NCBI nr
Match: XP_004146935.2 (flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa_014422 [Cucumis sativus])

HSP 1 Score: 645.2 bits (1663), Expect = 3.3e-181
Identity = 315/354 (88.98%), Postives = 336/354 (94.92%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGL GESSG+FPTGKTAQEKG SYVPNAYVIPAPHRPS+SPE AIVP+IDMASLRS+DS 
Sbjct: 3   MGLNGESSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSMSPEIAIVPIIDMASLRSSDSV 62

Query: 61  QRALAIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QR+LA EELRKACISLGFFQIINHGI++TVMEEAL QA EFF+LPLKEKMKYKSDDVC+A
Sbjct: 63  QRSLATEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLKEKMKYKSDDVCKA 122

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWID WPT+P +YREKMG+YTKEVRKVSLE+
Sbjct: 123 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGRYTKEVRKVSLEI 182

Query: 181 MEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAI ESL LSPTYLSSKMAEGVQVV VNCYPPCPQP VALGLPPHTDYSCITTVLQSSQ
Sbjct: 183 MEAINESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 242

Query: 241 GLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLE+MD ADGTWK+VPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 243 GLEVMDPADGTWKMVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 302

Query: 301 SLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKIQE 355
           SL MDEKMKPAEELV+E NPKKYK+SSFNDFLKFLSSNDLAEGK+F+ TLK+Q+
Sbjct: 303 SLGMDEKMKPAEELVNEHNPKKYKDSSFNDFLKFLSSNDLAEGKSFLNTLKLQD 356

BLAST of Spg002716 vs. NCBI nr
Match: XP_008453850.1 (PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa] >TYK16900.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa])

HSP 1 Score: 644.4 bits (1661), Expect = 5.6e-181
Identity = 317/354 (89.55%), Postives = 334/354 (94.35%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGL GE SG+FPTGKTAQEKG SYVPNAYVIPAPHRPSLSPE AIVP+IDMASLRS DS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRALAIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QR+LA+EELRKACISLGFFQIINHGI++TVMEEAL QA EFF+LPL EKMKYKSDDVC+A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWID WPT+P +YREKMGQYTKEVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL LSPTYLSSKMAEGVQVV VNCYPPCPQP VALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V KIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKIQE 355
           SL MDEKMKPAEELV+EQNPKKYKESSFNDFLKFLSSNDLAEG +FI TLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Spg002716 vs. NCBI nr
Match: XP_022140813.1 (flavanone 3-dioxygenase 3 [Momordica charantia])

HSP 1 Score: 624.8 bits (1610), Expect = 4.6e-175
Identity = 318/381 (83.46%), Postives = 332/381 (87.14%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGL GESSGSFPTG++AQEKG SYVPNAYVIPAPHR S S ETAI+P+IDMASLRSTDSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRALAIEELRKACISLGFF---------------------------QIINHGISKTVMEE 120
           QR+LAIEELR+ACI LGFF                           QIINH I++ VMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWID 180
           AL QAYEFFDLPLKEKMKYKSDDV RAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI 
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 YWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNCYPP 240
            WPTSP SYREKMG YT+EVRKV LEVMEAITESLGL PTYLSSKMAEGVQVV VNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHVEVL 300
           CPQPEVALGLPPHTDYSCITT+LQSS+GLEIMD ADGTWKLVP IDGALQVH+GDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFLK 355
           SNGLYKGVVHRATVNSESTRISITSLHSL MDEKMKPAEELVD+QNPKKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of Spg002716 vs. NCBI nr
Match: XP_038889497.1 (flavanone 3-dioxygenase 3 [Benincasa hispida])

HSP 1 Score: 624.8 bits (1610), Expect = 4.6e-175
Identity = 310/350 (88.57%), Postives = 328/350 (93.71%), Query Frame = 0

Query: 5   GESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSAQRAL 64
           G  +GSFPTGKTAQEKGFSYVPNAYVIPAPHR SLSPE A VP+IDMASLRS DSAQR+L
Sbjct: 9   GTGTGSFPTGKTAQEKGFSYVPNAYVIPAPHRASLSPEIATVPIIDMASLRSNDSAQRSL 68

Query: 65  AIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYG 124
           AI+ELRKACISLGFFQIINHGIS+TVMEEAL QA EFF+LPLKEKMKYKSDDV +AVRYG
Sbjct: 69  AIQELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVRKAVRYG 128

Query: 125 TSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAI 184
           TSLKDGVDKIKFWRVFLKHYAHPLEDWID WPT+P +YREKMGQYTK VRKVSLE+MEAI
Sbjct: 129 TSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKVVRKVSLEIMEAI 188

Query: 185 TESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEI 244
           TESL LSPTYLSSKMAEGVQVV VNCYPPCPQP VALGLPPHTDYSCITTVLQS+QGLEI
Sbjct: 189 TESLSLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSNQGLEI 248

Query: 245 MDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLAM 304
           MD ADGTWK+VPKI GALQVH+GDHVEVLSNGLYKGVVHRATVNSE  RISITSLHSL M
Sbjct: 249 MDPADGTWKMVPKIAGALQVHLGDHVEVLSNGLYKGVVHRATVNSERARISITSLHSLGM 308

Query: 305 DEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKIQE 355
           DEKMKPAEELVDEQNP KYK+SSF+DFLKFLSSNDLAEGK+FI TLKI++
Sbjct: 309 DEKMKPAEELVDEQNPIKYKDSSFDDFLKFLSSNDLAEGKSFINTLKIED 358

BLAST of Spg002716 vs. NCBI nr
Match: XP_022922584.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata])

HSP 1 Score: 620.5 bits (1599), Expect = 8.7e-174
Identity = 313/376 (83.24%), Postives = 330/376 (87.77%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGLKGESSGSF TG+TAQEKG +YVPNAYVIP P RPSLSPETAIVP+IDMASLRS DSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRALAIEELRKACISLGFF----------------------QIINHGISKTVMEEALGQA 120
           QR+L IE LRKACISLGFF                      QIINHGISKTVMEEAL QA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 YEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTS 180
            EFF+LPLKEKMKYKSDDV +AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+D WPT+
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPE 240
           P +YREKMGQYTKEVRKVSLE+ EAITESLGL PTYLSSKMAEGVQVV VNCYPPCPQP 
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITT+LQSSQGLEIMD ADG WK+VP +DG+LQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSN 355
           KGVVHRA VNSE TRISI+SLHSL MDEKMKPAEELVDEQNP KYKESSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of Spg002716 vs. ExPASy Swiss-Prot
Match: Q7XR84 (Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.4e-86
Identity = 158/322 (49.07%), Postives = 219/322 (68.01%), Query Frame = 0

Query: 31  IPAPHRPSLSPETAIVPVIDMASLRSTDSAQRALAIEELRKACISLGFFQIINHGISKTV 90
           IP    PS       +PVI++  L   D   R+  + ++ KAC  LG+FQ+I+HGIS++V
Sbjct: 8   IPQEQLPSQELHPPPMPVINLGHLSLDDPTVRSRVVNDIAKACRDLGYFQVISHGISQSV 67

Query: 91  MEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLED 150
           M+ A+  A EFF LP + K +Y SDD+ + VRY TS KDG   I   R FLKHYAHPL D
Sbjct: 68  MDGAIEAASEFFKLPNEIKKEYASDDIRQPVRYDTSSKDG---ISMSRAFLKHYAHPLCD 127

Query: 151 WIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNC 210
           W+ YWP  PP YRE M +Y  EVR V+L++MEAI E LG+   Y+  K  EG+Q+++VNC
Sbjct: 128 WLQYWPQQPPIYREYMAKYAVEVRVVALKLMEAILEGLGIGKEYMQEKFEEGLQLLSVNC 187

Query: 211 YPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHV 270
           YP   Q + ++GL  H+DY  +T +L S QGLE++D +  +WK+V ++  AL VHVGDH+
Sbjct: 188 YPKVSQSDTSIGLAAHSDYGLLTILLTSCQGLEVVDRSSNSWKVVQQLPHALHVHVGDHM 247

Query: 271 EVLSNGLYKGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFND 330
           EVLSNG  K VVHRA +N +  RIS+ S+H  A+ EK+  A+ELVDE+NP+KYKE+SFND
Sbjct: 248 EVLSNGRIKTVVHRAVLNPQEARISLASIHGFALHEKVSSAKELVDEENPQKYKENSFND 307

Query: 331 FLKFLSSNDLAEGKNFIGTLKI 353
           FL+ L++N     +NF+ +L++
Sbjct: 308 FLEHLTANMDNRQRNFLESLRM 326

BLAST of Spg002716 vs. ExPASy Swiss-Prot
Match: Q7XR83 (2-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD21 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.5e-83
Identity = 160/319 (50.16%), Postives = 216/319 (67.71%), Query Frame = 0

Query: 37  PSLSPETAIVPVIDMASLRSTDSAQRALAIEELRKACISLGFFQIINHGISKTVMEEALG 96
           P L P    +PVI++  L + DSA RALA+ ++  AC   G F+++NHGIS++ M  AL 
Sbjct: 19  PPLPPPPRPLPVINLGRL-TMDSASRALAVRDIVLACRERGCFEVVNHGISRSCMNGALE 78

Query: 97  QAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWP 156
            A EFF L  + K ++ SDD+ + +RY TS +DG   I   R FLKHYA+PL+DWI +WP
Sbjct: 79  AASEFFQLSTERKEEFASDDIRQPIRYDTSSRDG---ISMSRSFLKHYANPLDDWIKFWP 138

Query: 157 TSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQ 216
           T PP+YREKMG+Y  E ++VS+++MEAI + LGL P+YL  K+  GVQ V +N YP    
Sbjct: 139 TQPPTYREKMGEYAVETQRVSMQLMEAILQGLGLGPSYLQEKLEGGVQFVALNNYPQSSA 198

Query: 217 PEV-ALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSN 276
            +   +GL PH+DY  +T +LQSS GLE+M   D  W  VP I GAL VHVGDH+EVLSN
Sbjct: 199 KKADKIGLAPHSDYGFLTILLQSSPGLEVMHHEDDAWTSVPAIPGALHVHVGDHLEVLSN 258

Query: 277 GLYKGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFL 336
           G  K +VHRA +N   +RISI S+H L+MDE++  AEELVDE +PK Y+ SSF DFL FL
Sbjct: 259 GQLKSLVHRAVLNPNESRISIASIHGLSMDEEVHCAEELVDEHHPKMYRGSSFQDFLDFL 318

Query: 337 SSNDLAEGKNFIGTLKIQE 355
            +N +   K ++ +L+I +
Sbjct: 319 PAN-MNRYKRYVESLRIDK 332

BLAST of Spg002716 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 5.7e-67
Identity = 134/327 (40.98%), Postives = 195/327 (59.63%), Query Frame = 0

Query: 19  EKGFSYVPNAYVIPAPHRPSLSPETAI-------VPVIDMASLRSTDSAQRALAIEELRK 78
           + G + +P  YV+PA  RP  +   A        +PV+D++ LR    ++R   +  L  
Sbjct: 46  DSGITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRV--PSERGAVLRTLDA 105

Query: 79  ACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGV 138
           AC   GFFQ++NHG+   V+   L  A  FF+LP  E+ +Y S DV   VRYGTS     
Sbjct: 106 ACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVR 165

Query: 139 DKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLS 198
           D +  WR FLK    PL   ++ WPTSP   RE   +Y +  ++V +EVMEA  E+LG+ 
Sbjct: 166 DAVLCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVG 225

Query: 199 PTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSS-QGLEIMDTADG 258
              +   +A G Q++TVNCYP CPQPE+ LG+PPH+DY  +T VLQ    GL++M    G
Sbjct: 226 GGGVMEDLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHA--G 285

Query: 259 TWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLAMDEKMKP 318
            W  V  + G+  V+VGDH+E+LSNG Y+ V+HR  VNS   R+S+ S HS+A +  + P
Sbjct: 286 EWLTVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASFHSVAPERVVSP 345

Query: 319 AEELVDEQNPKKYKESSFNDFLKFLSS 338
           A EL+D+++P++Y ++    FL +L+S
Sbjct: 346 APELIDDRHPRRYMDTDLATFLAYLAS 368

BLAST of Spg002716 vs. ExPASy Swiss-Prot
Match: Q651B6 (Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC2 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.1e-61
Identity = 145/363 (39.94%), Postives = 211/363 (58.13%), Query Frame = 0

Query: 5   GESSGS--FPTG-KTAQEKGFSYVPNAYVIPAPHRPS--LSPETAIVPVIDMASLRSTDS 64
           GE SGS  +  G +   + G + +P  YV+P   RP+  ++P  A++PV+D+A+LR+ D 
Sbjct: 13  GEGSGSSEWELGVRQLCDSGITTLPARYVLPPADRPARYVTP-PALLPVVDLAALRARDP 72

Query: 65  AQRALAIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCR 124
            Q A     L  AC   GFFQ++NHG+    M  A  +   FFDLPL  + +Y S D+  
Sbjct: 73  CQLA----ALHAACRDYGFFQLLNHGVPPDAMLYAARRF--FFDLPLPARKRYMSADIRA 132

Query: 125 AVRYGTSLKDGVDKIKFWRVFLK---HYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKV 184
           AVRYGTS     D +  WR FLK        L D +  WP +P   R     Y    +++
Sbjct: 133 AVRYGTSFNQLNDAVLSWRDFLKLLIRDTRRLADVLPSWPDAPDDLRPAAAAYATACQRL 192

Query: 185 SLEVMEAITESLGL--SPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITT 244
             E+MEA  ++LG+      L  +   G Q++ VNC+P CP+PE+ LG+PPH+DY  +T 
Sbjct: 193 FRELMEAALDALGIVRCRRQLLEECDAGSQMMMVNCFPACPEPELTLGMPPHSDYGLLTI 252

Query: 245 VLQSS-QGLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTR 304
           +LQ   +GLE+     G W +V  + GA+ V+VGDH+E+LSNGLY+ V+HR  VN    R
Sbjct: 253 LLQDEVRGLEVSYGDGGGWAVVEPLPGAVVVNVGDHLEILSNGLYRSVLHRVRVNGRRAR 312

Query: 305 ISITSLHSLAMDEKMKPAEELVDEQ--NPKKYKESSFNDFLKFLSSNDLAEG--KNFIGT 353
           +S+ SLHSLA +  + PA ELVDEQ   P++Y ++    FL +L+S   AEG  K+F+ +
Sbjct: 313 VSVASLHSLAAERVIGPAAELVDEQRGRPRRYMDTDMAAFLAYLAS---AEGNHKSFLHS 365

BLAST of Spg002716 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.8e-58
Identity = 115/321 (35.83%), Postives = 189/321 (58.88%), Query Frame = 0

Query: 25  VPNAYVIPAPHRPSLSPETAI--VPVIDMASLRSTDSAQRALAIEELRKACISLGFFQII 84
           +P  YV P   RP LS  + +   P+ID++      S  R+  I+++ +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E +  A EFF + ++EKMK  SDD  +  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEG 204
            + +P+  +++ WP++PPS++E + +Y++EVR+V  ++ E I+ESLGL   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGAL 264
            Q + VN YPPCP+PE+  GLP HTD + +T +LQ +    +    DG W  V     A 
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 254

Query: 265 QVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSL---HSLAMDEKMKPAEELVDEQN 324
            +++GD ++ LSNG+YK V HRA  N+E+ R+S+ S       A+    KP  E  D++ 
Sbjct: 255 VINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDET 314

Query: 325 PKKYKESSFNDFLKFLSSNDL 341
              YK+ ++ ++ K   S +L
Sbjct: 315 KPVYKDFTYAEYYKKFWSRNL 329

BLAST of Spg002716 vs. ExPASy TrEMBL
Match: A0A5A7TMH3 (Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00340 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.7e-181
Identity = 317/354 (89.55%), Postives = 334/354 (94.35%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGL GE SG+FPTGKTAQEKG SYVPNAYVIPAPHRPSLSPE AIVP+IDMASLRS DS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRALAIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QR+LA+EELRKACISLGFFQIINHGI++TVMEEAL QA EFF+LPL EKMKYKSDDVC+A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWID WPT+P +YREKMGQYTKEVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL LSPTYLSSKMAEGVQVV VNCYPPCPQP VALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V KIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKIQE 355
           SL MDEKMKPAEELV+EQNPKKYKESSFNDFLKFLSSNDLAEG +FI TLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Spg002716 vs. ExPASy TrEMBL
Match: A0A1S3BX86 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.7e-181
Identity = 317/354 (89.55%), Postives = 334/354 (94.35%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGL GE SG+FPTGKTAQEKG SYVPNAYVIPAPHRPSLSPE AIVP+IDMASLRS DS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRALAIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRA 120
           QR+LA+EELRKACISLGFFQIINHGI++TVMEEAL QA EFF+LPL EKMKYKSDDVC+A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWID WPT+P +YREKMGQYTKEVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL LSPTYLSSKMAEGVQVV VNCYPPCPQP VALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V KIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKIQE 355
           SL MDEKMKPAEELV+EQNPKKYKESSFNDFLKFLSSNDLAEG +FI TLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Spg002716 vs. ExPASy TrEMBL
Match: A0A6J1CIV6 (flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.2e-175
Identity = 318/381 (83.46%), Postives = 332/381 (87.14%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGL GESSGSFPTG++AQEKG SYVPNAYVIPAPHR S S ETAI+P+IDMASLRSTDSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRALAIEELRKACISLGFF---------------------------QIINHGISKTVMEE 120
           QR+LAIEELR+ACI LGFF                           QIINH I++ VMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWID 180
           AL QAYEFFDLPLKEKMKYKSDDV RAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI 
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 YWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNCYPP 240
            WPTSP SYREKMG YT+EVRKV LEVMEAITESLGL PTYLSSKMAEGVQVV VNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHVEVL 300
           CPQPEVALGLPPHTDYSCITT+LQSS+GLEIMD ADGTWKLVP IDGALQVH+GDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFLK 355
           SNGLYKGVVHRATVNSESTRISITSLHSL MDEKMKPAEELVD+QNPKKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of Spg002716 vs. ExPASy TrEMBL
Match: A0A6J1E3T8 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430549 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.2e-174
Identity = 313/376 (83.24%), Postives = 330/376 (87.77%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGLKGESSGSF TG+TAQEKG +YVPNAYVIP P RPSLSPETAIVP+IDMASLRS DSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRALAIEELRKACISLGFF----------------------QIINHGISKTVMEEALGQA 120
           QR+L IE LRKACISLGFF                      QIINHGISKTVMEEAL QA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 YEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTS 180
            EFF+LPLKEKMKYKSDDV +AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+D WPT+
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPE 240
           P +YREKMGQYTKEVRKVSLE+ EAITESLGL PTYLSSKMAEGVQVV VNCYPPCPQP 
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITT+LQSSQGLEIMD ADG WK+VP +DG+LQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSN 355
           KGVVHRA VNSE TRISI+SLHSL MDEKMKPAEELVDEQNP KYKESSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of Spg002716 vs. ExPASy TrEMBL
Match: A0A6J1JAE6 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LOC111482715 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.7e-171
Identity = 311/381 (81.63%), Postives = 329/381 (86.35%), Query Frame = 0

Query: 1   MGLKGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSA 60
           MGL+GESSGSF TG+TAQEKG +YVPNAYVIP P RPSLSPETAIVP+IDMASLRS DSA
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRALAIEELRKACISLGFF----------------------------QIINHGISKTVME 120
           +R+LAIE LRKACISLGFF                            QIINHGISKTVME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI 180
           EAL QA EFF+LPLKEKMKYKSDDV +AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 DYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEGVQVVTVNCYP 240
           D WPT+P +YREKMGQYTKEVRKVSLE+ EAITESLGL P YLSSKMAEGVQVV VNCYP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYP 240

Query: 241 PCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGALQVHVGDHVEV 300
           PCPQP VALGLPPHTDYSCITT+LQSSQGLEIMD ADG WK+VP +DG+LQVHVGDHVEV
Sbjct: 241 PCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV 300

Query: 301 LSNGLYKGVVHRATVNSESTRISITSLHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFL 354
           LSNGLYKGVVHRA VNSE TRISI+SLHSL MDEKMKPAEELVDEQNP KYKESSFNDFL
Sbjct: 301 LSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFL 360

BLAST of Spg002716 vs. TAIR 10
Match: AT5G07480.1 (KAR-UP oxidoreductase 1 )

HSP 1 Score: 397.9 bits (1021), Expect = 8.6e-111
Identity = 196/356 (55.06%), Postives = 254/356 (71.35%), Query Frame = 0

Query: 4   KGESSGSFPTGKTAQEKGFSYVPNAYVIPAPHRPSLSPETAIVPVIDMASLRSTDSAQRA 63
           KGE S +F  G +AQE+   YVP+ YV+P   +P  S  + IVP ID++ L+  D  +R 
Sbjct: 3   KGEDSSTFKMGNSAQERSLPYVPDCYVVPPSSKPCDS-NSGIVPTIDVSRLKGGDDERRG 62

Query: 64  LAIEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRY 123
                            I+NHGI++ ++++AL  A  FF+LP KEK ++ S+DV   VRY
Sbjct: 63  -----------------IVNHGINQNILDDALEVANSFFELPAKEKKQFMSNDVYAPVRY 122

Query: 124 GTSLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEA 183
            TSLKDG+D I+FWR+FLKHYAHPL  WI  WP +PP YREKMG++ +EVRK+S+E+M A
Sbjct: 123 STSLKDGLDTIQFWRIFLKHYAHPLHRWIHLWPENPPGYREKMGKFCEEVRKLSIELMGA 182

Query: 184 ITESLGLSPTYLSSKMAE-GVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGL 243
           ITESLGL   YLSS+M E G+QV+TVNCYPPCP PE ALGLPPH+DYSCIT +LQ+  GL
Sbjct: 183 ITESLGLGRDYLSSRMDENGMQVMTVNCYPPCPDPETALGLPPHSDYSCITLLLQNLDGL 242

Query: 244 EIMDT----ADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITS 303
           +I D       G W  VP++ G L+VH+GDHVEVLSNGLYK +VH+ T+N E TRIS+ S
Sbjct: 243 KIFDPMAHGGSGRWVGVPQVTGVLKVHIGDHVEVLSNGLYKSIVHKVTLNEEKTRISLAS 302

Query: 304 LHSLAMDEKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKIQE 355
           LHSL MD+KM    ELV+++NP +YKESSFNDFL FL  ND+++G  FI TL+I++
Sbjct: 303 LHSLGMDDKMSVPRELVNDENPVRYKESSFNDFLDFLVKNDISQGDRFIDTLRIKD 340

BLAST of Spg002716 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.9e-94
Identity = 169/345 (48.99%), Postives = 228/345 (66.09%), Query Frame = 0

Query: 11  FPTGKTAQEKGFSYVPNAYVIPAPHRPSLSP----ETAIVPVIDMASLRSTDSAQRALAI 70
           F +     E G  +VP  Y++P   RP L P     T  +PVID++ L   D   R   I
Sbjct: 13  FTSAMELTESGDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLH--DPLLRLCVI 72

Query: 71  EELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTS 130
            E+  AC   GFFQ+INHGIS  V+++A   A  FFDLP  EKM   SD+    VRYGTS
Sbjct: 73  HEIELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVRYGTS 132

Query: 131 LKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITE 190
           +    D++ +WR F+KHY+HPL +WI+ WP++PP Y+EK+G+Y +    +  +++EAI+E
Sbjct: 133 INHSTDRVHYWRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISE 192

Query: 191 SLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMD 250
           SLGL   YL  ++ EG QV+ VNCYP CP+PE+ALG+PPH+DY  +T +LQSS+GL+I D
Sbjct: 193 SLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKD 252

Query: 251 TADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLAMDE 310
             +  W  VP I+GAL V +GD VEV+SNG+YK VVHR TVN +  R+S  SLHSL M +
Sbjct: 253 -CNNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHK 312

Query: 311 KMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLK 352
           K+ PA +LV+E  P  Y E SFNDFL ++S ND+ + K FI TL+
Sbjct: 313 KISPATQLVNENKPAAYGEFSFNDFLDYISRNDITQ-KRFIDTLR 353

BLAST of Spg002716 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.5e-94
Identity = 171/346 (49.42%), Postives = 233/346 (67.34%), Query Frame = 0

Query: 10  SFPTGKTAQEKGFSYVPNAYVIPAPHRP----SLSPETAIVPVIDMASLRSTDSAQRALA 69
           SF +  T    G   VP+ YV+P   RP    SL      +PVID++ L       R+LA
Sbjct: 12  SFTSAMTLTNSGVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLH--QPFLRSLA 71

Query: 70  IEELRKACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGT 129
           I E+  AC   GFFQ+INHGI  +V+ +AL  A +FFDLP++EKM   S +V   VRYGT
Sbjct: 72  IHEISMACKEFGFFQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGT 131

Query: 130 SLKDGVDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAIT 189
           SL    D++ +WR F+KHY+HPL  WID WP++PP Y++K+G+Y +    +  +++EAI+
Sbjct: 132 SLNHSTDRVHYWRDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAIS 191

Query: 190 ESLGLSPTYLSSKMAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIM 249
           ESLGL   YL  ++ EG QV+ VNCYP CP+PE+ALG+PPH+D+S +T +LQSS+GL+IM
Sbjct: 192 ESLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIM 251

Query: 250 DTADGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLAMD 309
           D  +  W  VP I+GAL V +GD VEV+SNG+YK V+HR TVN E  R+S  SLHSL + 
Sbjct: 252 D-CNKNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLH 311

Query: 310 EKMKPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLK 352
           +K+ PA +LV+  N   Y E SFNDFL ++SSND  + + FI T+K
Sbjct: 312 KKISPAPKLVNPNNAPAYGEFSFNDFLNYISSNDFIQ-ERFIDTIK 353

BLAST of Spg002716 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.6e-72
Identity = 140/344 (40.70%), Postives = 209/344 (60.76%), Query Frame = 0

Query: 19  EKGFSYVPNAYVIPAPHRPSLSPETAIV--------PVIDMASLRSTDSAQRALAIEELR 78
           E G + VP  Y+ P P RP L+    ++        P+ID A L   +   R   +  + 
Sbjct: 26  ENGLTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAELLGPN---RPHVLRTIA 85

Query: 79  KACISLGFFQIINHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDG 138
           +AC + GFFQ++NHG+   V +  +     FF+LP +E+ KY S D+   VRYGTS    
Sbjct: 86  EACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYGTSFNQI 145

Query: 139 VDKIKFWRVFLKHYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGL 198
            D +  WR FLK YAHPL D++ +WP+SP  +R     Y KE +++   +++AI ESL +
Sbjct: 146 KDNVFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAILESLEI 205

Query: 199 SPTYLSSK-MAEGVQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSS-QGLEIMDTA 258
             +  ++K + EG QVV VNCYPPCP+PE+ LG+PPH+DY  +T +LQ   +GL+I+   
Sbjct: 206 DGSDEAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEGLQILYRD 265

Query: 259 DGTWKLVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLAMDEKM 318
           +  W  V  I G+  V+VGDH+E+ SNG YK V+HR  VNS   RIS+ SLHS  +   +
Sbjct: 266 E--WVTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLVNSTKPRISVASLHSFPLTSVV 325

Query: 319 KPAEELVDEQNPKKYKESSFNDFLKFLSSNDLAEGKNFIGTLKI 353
           KP+ +LVD+ NP +Y ++ F  FL++++S +  + KNF+ + KI
Sbjct: 326 KPSPKLVDKHNPSQYMDTDFTTFLQYITSRE-PKWKNFLESRKI 363

BLAST of Spg002716 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 227.3 bits (578), Expect = 2.0e-59
Identity = 115/321 (35.83%), Postives = 189/321 (58.88%), Query Frame = 0

Query: 25  VPNAYVIPAPHRPSLSPETAI--VPVIDMASLRSTDSAQRALAIEELRKACISLGFFQII 84
           +P  YV P   RP LS  + +   P+ID++      S  R+  I+++ +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALGQAYEFFDLPLKEKMKYKSDDVCRAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E +  A EFF + ++EKMK  SDD  +  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWIDYWPTSPPSYREKMGQYTKEVRKVSLEVMEAITESLGLSPTYLSSKMAEG 204
            + +P+  +++ WP++PPS++E + +Y++EVR+V  ++ E I+ESLGL   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVTVNCYPPCPQPEVALGLPPHTDYSCITTVLQSSQGLEIMDTADGTWKLVPKIDGAL 264
            Q + VN YPPCP+PE+  GLP HTD + +T +LQ +    +    DG W  V     A 
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 254

Query: 265 QVHVGDHVEVLSNGLYKGVVHRATVNSESTRISITSL---HSLAMDEKMKPAEELVDEQN 324
            +++GD ++ LSNG+YK V HRA  N+E+ R+S+ S       A+    KP  E  D++ 
Sbjct: 255 VINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDET 314

Query: 325 PKKYKESSFNDFLKFLSSNDL 341
              YK+ ++ ++ K   S +L
Sbjct: 315 KPVYKDFTYAEYYKKFWSRNL 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146935.23.3e-18188.98flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa... [more]
XP_008453850.15.6e-18189.55PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protei... [more]
XP_022140813.14.6e-17583.46flavanone 3-dioxygenase 3 [Momordica charantia][more]
XP_038889497.14.6e-17588.57flavanone 3-dioxygenase 3 [Benincasa hispida][more]
XP_022922584.18.7e-17483.24LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q7XR842.4e-8649.07Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1... [more]
Q7XR832.5e-8350.162-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q6YYX95.7e-6740.98Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Q651B66.1e-6139.94Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japoni... [more]
Q9FLV02.8e-5835.83Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7TMH32.7e-18189.55Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BX862.7e-18189.55protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 S... [more]
A0A6J1CIV62.2e-17583.46flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV... [more]
A0A6J1E3T84.2e-17483.24LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JAE65.7e-17181.63LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G07480.18.6e-11155.06KAR-UP oxidoreductase 1 [more]
AT3G60290.11.9e-9448.992-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G44800.12.5e-9449.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G36690.14.6e-7240.702-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G24530.12.0e-5935.832-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 14..339
e-value: 1.7E-88
score: 299.1
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 207..300
e-value: 1.9E-21
score: 76.5
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 46..155
e-value: 5.4E-24
score: 85.2
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 10..339
NoneNo IPR availablePANTHERPTHR47991:SF67KAR-UP OXIDOREDUCTASE 1coord: 10..339
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 17..337
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 199..302
score: 10.44223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002716.1Spg002716.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding