Spg002701 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002701
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold6: 5774696 .. 5778607 (-)
RNA-Seq ExpressionSpg002701
SyntenySpg002701
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATTCAAGAGAGCCATTGTTGGAGCTTGGTTTTGCAGGAAAGGAAGAAAGATGGTGGAGGAGAGTTCTGGACATGGAGGAAACAAAGCTTCAACTTCAATTTTCCCTTCCAATGATTCTTACAAATGTATTCTATTACATGATACCCTTGGTCTCTGTAATGTTTGCTGGTCATCTTGGTGAACTTGAGCTTGCTGCTGCTACTCTTGCTAATACTTTGGCTAGTGTCACTGGCTTTGCTTTCATGGTATCTCTCTCTCTTTCTCTCTCTTTTCCTTTGCTTTCATGATTTGGGGAAATGGGTCTTTAAATCCCTAGTCTCTGATGCTAAGAGATGAAAAATGGAAGAAATTGGAAGTGGGTTTAGTCAAGAAACTGAGTTTGGAGCTTGCTAAAGTGGGTTAGATTTTGAGTTTTCTTTTTTTCTTCTTGTTGGGGATGTTTTAATTTCTGCCATTGCTGTCCACCAAACTTGCAGCATTGGGATTCTGTGCTTCTTTTTAAGGTGAAAATCAGAATGAAAATAGGAGTTTTGTGTCAGTTAATGGAACAAAATCACTATCTTAGAGAAGATTAGTGTATTTCTATTACTGCTTCATCAATAGATTGAAGTGTGATTCTGCTAGAATTCTTGTAAAGTTGATGGTTAACTTTCTCAACAAGTTGAAGGGAAGAAAGCAAGGAGAGGATGACTGAAAAAATTGTTTAAAGTAGGCTAAGAGTTGCAAATTTAATTGTTGAACTTTGATGGGTCTATCAATTTAGTCCCTGAAGTTTAAAAGTTTGTGATGCTAAATAATTGTGTGTGACATGCCTTCTAGATAAAGTTGGCTGATTTGGTTGGACATGGCAGACTGGTAAGTATAGAAAGTGGATGTGCTAAATTTAGCCTTTGAGGGTATGTTGCACATATCACTTTATTTTGTTCAATTGTTCAAATAGATAACTATAGAAAGTGGATGTGATCAATTAAGCCTTGAGGGAATGTATTGCAAACATGCCACTCTCTTTTGTCCAATGGTTCATTTTTTGGCTAGTTATGTCTATAATGTCATGCTAAATTGGGAAATGTTTATCTAACAGACATGTCACTTATCATTTAGTAAACCGTGTTGACAACATCTATATACGATCACAATTCACTTTTCATAGACATGTTTGGAATTTTTTTAATTCAGGAACTAAATAGACATACCAATGAAAGTTTAAGGAAATTGGCAATTTAAAACATATGAACCCAACATATTAAAAGATCCCCCTGCTTGGTGGTATGATCTGTTGTAATAATCTCTAATATTTGCAATATTTCAGCACATAGCATATAAGAAATGGAGTATTCAACTATTATGAGGCACCTAAGGCAGCAGATCTCAAGGTGAGGATGTCAAAATCTGTTGCCCATTTTCTTGCAATTAGGATAAGATTGTCCTAACTCAAAGCTCATTAGCATCACTAAAACTCAACTTGGCCTTTGTAAAATCTATTCTAAGTTCTAGAGAGAATTGAAATGCGATGATAACTCAGCTTTATCTGTTTTGGTTATAGTTGTGTACCTCTTTTTTCTGTAACTTTCTTCTTGTTCTTTAGACAGGACTGAGCGGTGCACTTGAAACGCTTTGCGGGCAAGCATTTGGCGGGAAGTTTTATGGGAAGATGGGGTTGTATCTTCAAGCCTCTTGCATCTTATCATTCTTTTGTTCCATCATTGTATCAGTTTTGTGGATTTACACACAGCCAATACTAGTCTTGCTCCATCAAGAACTGGAGATTTCGAGGGTATCAGCAATGTACATGAAGTTTCTCATCCCAGGATTATTTGCATATGGCTTACTGCAGAACATCCTGAGGTTTCTTCAGACACAATCTGTTGTCATGCCTCTGGTTTTCTTCTCTGCAGTGCCAATGTTCATCCACATAGCTATTGCATACGGTTTGGTTCACTGGACACGTCTCGGTTTCATCGGAGCTCCATTGGCTGCTTCAATCTCATTATCGATGTCATGCCTGATTTTGGCTATATATGTACTTAAAGCAAAGAGGTTTGAGGAATCTTGGAAAGGCTTCTCATTAGAAGCTTTTAGCTACAGTCTTTCAAGCTTAAAACTAGCTATTCCATCAGCAGCAATGTTGTGGTAAGCATCCTCATCTCTATGCCTTAAATGCAACTTATCGAATAACAACAGTTAATATTCGAAACCGCTACCACCTGTTCGATCAATAACCAACTCTGCAAATTTTAACTCACCCCAATATCTTGTTCATAACTCATGGACCTTTTGTGCCTTTCATTGTTCAAATTGGTTGAAGTTTGGAGTATTGGGCGGTCGAGGTTATGGTATTCTTAGCCGGGTTGATGCCAAACCCGGAAACGAGTACGTCACTCATAGCAATGTGGTAAGTAAATCTATTTTTCCATGTCCTTCTGATACAAATACTGTCGTTCCATTTTTCTTACTTGGTTTTCATTTTCTGATCCTCTAGTGACAACACACAAACCATTGCCTACATGGTCACTTGTGGTCTCAGTGCTGCAGCAAGGTTCGTTATTGCAAAGTTTGAATTATTGTTCTAGTTCCTGTGGTTTCAATAAGAGTTTCACTGTGCCCTATGGTTTAGATATGTCTCTTAAAACTTTCTTACTGTTACCAAATGTTAATAGTTTTCTTACCCGACAATCGTCTCTCTCTACAATGCTAGAAAAGGAAGTATCAGGTAAGAAAACTGTTAACATTTCGTAACAACGTAAACATTCCTTAAGGTTTAACAAAAACTATAGTGACTAGAGTGAAACTTTAGACCATGGGGTAATTTAACTAGCAAGAAGGAGAAAACCCTTAAAATTGTTGGGTTCTTTTCTTTTCCCCCTTAGTTTTATGAAAGTTTTCCTTCTAAAACAAATGACACTTTGTGCAGCACAAGGGTCTCAAATGAGTTGGGAGCAGGCAATGTTGACAGAGCCAAAACTGCTATGTTTGCAACTCTCAAGCTTTCTGTTCTCCTCCCCCTGCTTGCTGTTTTGGCACTTGCATTTGGACAGAACACCTGGGCTAGTTTCTTCAGCAACAGCATTACAATGATGGACGAGTTTTCTTCCATGGCACCGTTTCTCGCTGTCTCGATAACGTTGGACTCGGTGCAAGGCGCCATATCAGGTTCGGTACTAAAAAGTTTCATTCCCATTCAGCACTGCTCTTTTTCTCATAATTCTCATTCAACTGCAGGGGTGGCTAGAGGCTATGGTTGGCAGCACCTGGCTGTATACATAAACTTGTCCACGTTCTACTTCGTCGGTGTAACGATATCGATCCTTCTTGGGTTTAAGTTTAGATTATATGCTAAGGTGACAATCTTTACACACAATGTTCATGACTACATATTCAAAGACATTGGTATGTATCTTTGATCTTCATTTTTTGTTGATTTGATACTGATGCAGGGTTTATGGATCGGATACATCTGCGGTCTCTCGTCGCAAACCGCTTGTCTTCTTTCGGTCGTTTTGTGTGTAAACTGGATTAAAATGGATCAATCTGATAATGAAGCAAAAGATGTTCCATTTCTGGTTTAGTGGGAAGTTTTAGCAATGATAGTTTAGAAAGAAATAATTGAAAGGAAACATTCAAACCCCGTTTGATAACAATTTTGTTATTGGTTTTCTATTTTTGAAAATTAGGCTTATATTATTAATGCCTTCTTTGTTTAAATTTTCCCTTTTCAAAAAAACACTTGGAAAATCTTCCCTAAATTTTAAAACCAGAAAACAGGTTTTTGAAAACTTTTCTTTTGAGTTTTTAAAATTTGGTTGCTATATTTAAAATGATTTTTAAACTGGATTAGAAAATAAAGAAAAAGGTAAATAAATAAGCATAATTTATAGAGAACTAAAAACAAAAACATTATCAAACAG

mRNA sequence

ATGGAAGATTCAAGAGAGCCATTGTTGGAGCTTGGTTTTGCAGGAAAGGAAGAAAGATGGTGGAGGAGAGTTCTGGACATGGAGGAAACAAAGCTTCAACTTCAATTTTCCCTTCCAATGATTCTTACAAATGTATTCTATTACATGATACCCTTGGTCTCTGTAATGTTTGCTGGTCATCTTGGTGAACTTGAGCTTGCTGCTGCTACTCTTGCTAATACTTTGGCTAGTGTCACTGGCTTTGCTTTCATGCATTGGGATTCTGTGCTTCTTTTTAAGATAAAGTTGGCTGATTTGGTTGGACATGGCAGACTGACAGGACTGAGCGGTGCACTTGAAACGCTTTGCGGGCAAGCATTTGGCGGGAAGTTTTATGGGAAGATGGGGTTGTATCTTCAAGCCTCTTGCATCTTATCATTCTTTTGTTCCATCATTGTATCAGTTTTGTGGATTTACACACAGCCAATACTAGTCTTGCTCCATCAAGAACTGGAGATTTCGAGGGTATCAGCAATGTACATGAAGTTTCTCATCCCAGGATTATTTGCATATGGCTTACTGCAGAACATCCTGAGGTTTCTTCAGACACAATCTGTTGTCATGCCTCTGGTTTTCTTCTCTGCAGTGCCAATGTTCATCCACATAGCTATTGCATACGGTTTGGTTCACTGGACACGTCTCGGTTTCATCGGAGCTCCATTGGCTGCTTCAATCTCATTATCGATGTCATGCCTGATTTTGGCTATATATGTACTTAAAGCAAAGAGGTTTGAGGAATCTTGGAAAGGCTTCTCATTAGAAGCTTTTAGCTACAGTCTTTCAAGCTTAAAACTAGCTATTCCATCAGCAGCAATGTTGTGTTTGGAGTATTGGGCGGTCGAGGTTATGGTATTCTTAGCCGGGTTGATGCCAAACCCGGAAACGAGTACGTCACTCATAGCAATGTGTGACAACACACAAACCATTGCCTACATGGTCACTTGTGGTCTCAGTGCTGCAGCAAGCACAAGGGTCTCAAATGAGTTGGGAGCAGGCAATGTTGACAGAGCCAAAACTGCTATGTTTGCAACTCTCAAGCTTTCTGTTCTCCTCCCCCTGCTTGCTGTTTTGGCACTTGCATTTGGACAGAACACCTGGGCTAGTTTCTTCAGCAACAGCATTACAATGATGGACGAGTTTTCTTCCATGGCACCGTTTCTCGCTGTCTCGATAACGTTGGACTCGGTGCAAGGCGCCATATCAGGGGTGGCTAGAGGCTATGGTTGGCAGCACCTGGCTGTATACATAAACTTGTCCACGTTCTACTTCGTCGGTGTAACGATATCGATCCTTCTTGGGTTTAAGTTTAGATTATATGCTAAGGGTTTATGGATCGGATACATCTGCGGTCTCTCGTCGCAAACCGCTTGTCTTCTTTCGGTCGTTTTGTGTGTAAACTGGATTAAAATGGATCAATCTGATAATGAAGCAAAAGATGTTCCATTTCTGGTTTAG

Coding sequence (CDS)

ATGGAAGATTCAAGAGAGCCATTGTTGGAGCTTGGTTTTGCAGGAAAGGAAGAAAGATGGTGGAGGAGAGTTCTGGACATGGAGGAAACAAAGCTTCAACTTCAATTTTCCCTTCCAATGATTCTTACAAATGTATTCTATTACATGATACCCTTGGTCTCTGTAATGTTTGCTGGTCATCTTGGTGAACTTGAGCTTGCTGCTGCTACTCTTGCTAATACTTTGGCTAGTGTCACTGGCTTTGCTTTCATGCATTGGGATTCTGTGCTTCTTTTTAAGATAAAGTTGGCTGATTTGGTTGGACATGGCAGACTGACAGGACTGAGCGGTGCACTTGAAACGCTTTGCGGGCAAGCATTTGGCGGGAAGTTTTATGGGAAGATGGGGTTGTATCTTCAAGCCTCTTGCATCTTATCATTCTTTTGTTCCATCATTGTATCAGTTTTGTGGATTTACACACAGCCAATACTAGTCTTGCTCCATCAAGAACTGGAGATTTCGAGGGTATCAGCAATGTACATGAAGTTTCTCATCCCAGGATTATTTGCATATGGCTTACTGCAGAACATCCTGAGGTTTCTTCAGACACAATCTGTTGTCATGCCTCTGGTTTTCTTCTCTGCAGTGCCAATGTTCATCCACATAGCTATTGCATACGGTTTGGTTCACTGGACACGTCTCGGTTTCATCGGAGCTCCATTGGCTGCTTCAATCTCATTATCGATGTCATGCCTGATTTTGGCTATATATGTACTTAAAGCAAAGAGGTTTGAGGAATCTTGGAAAGGCTTCTCATTAGAAGCTTTTAGCTACAGTCTTTCAAGCTTAAAACTAGCTATTCCATCAGCAGCAATGTTGTGTTTGGAGTATTGGGCGGTCGAGGTTATGGTATTCTTAGCCGGGTTGATGCCAAACCCGGAAACGAGTACGTCACTCATAGCAATGTGTGACAACACACAAACCATTGCCTACATGGTCACTTGTGGTCTCAGTGCTGCAGCAAGCACAAGGGTCTCAAATGAGTTGGGAGCAGGCAATGTTGACAGAGCCAAAACTGCTATGTTTGCAACTCTCAAGCTTTCTGTTCTCCTCCCCCTGCTTGCTGTTTTGGCACTTGCATTTGGACAGAACACCTGGGCTAGTTTCTTCAGCAACAGCATTACAATGATGGACGAGTTTTCTTCCATGGCACCGTTTCTCGCTGTCTCGATAACGTTGGACTCGGTGCAAGGCGCCATATCAGGGGTGGCTAGAGGCTATGGTTGGCAGCACCTGGCTGTATACATAAACTTGTCCACGTTCTACTTCGTCGGTGTAACGATATCGATCCTTCTTGGGTTTAAGTTTAGATTATATGCTAAGGGTTTATGGATCGGATACATCTGCGGTCTCTCGTCGCAAACCGCTTGTCTTCTTTCGGTCGTTTTGTGTGTAAACTGGATTAAAATGGATCAATCTGATAATGAAGCAAAAGATGTTCCATTTCTGGTTTAG

Protein sequence

MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGHLGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAFGGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPGLFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISLSMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKLSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGYGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNWIKMDQSDNEAKDVPFLV
Homology
BLAST of Spg002701 vs. NCBI nr
Match: XP_038903838.1 (protein DETOXIFICATION 18-like [Benincasa hispida])

HSP 1 Score: 778.9 bits (2010), Expect = 2.7e-221
Identity = 416/497 (83.70%), Postives = 437/497 (87.93%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           M+D REPLLEL    KE RWW+RVL+MEE KLQL FSLP+ILTNVFYY+I LVSVMFAGH
Sbjct: 1   MDDCREPLLEL----KEGRWWKRVLEMEEAKLQLLFSLPLILTNVFYYLITLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
           LGELELAAATLANTLASVTGFAFM                     TGLSG+LETLCGQAF
Sbjct: 61  LGELELAAATLANTLASVTGFAFM---------------------TGLSGSLETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G KFY KMGLYLQ SCILSFFCSI++SV+WIYT+ ILVLLHQE  ISR+SAMYMKFLIPG
Sbjct: 121 GRKFYEKMGLYLQGSCILSFFCSIVISVMWIYTEQILVLLHQEPGISRLSAMYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPLVFFSA+PMFIHI  AYGLVHWT LGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSALPMFIHIPTAYGLVHWTHLGFNGAPLAVSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MS LILAIYVLKA +FE+SW+GFSLEAFSYSLSSLKLAIPSAAM+CLEYWAVEVMVFLA
Sbjct: 241 WMSFLILAIYVLKANKFEKSWQGFSLEAFSYSLSSLKLAIPSAAMVCLEYWAVEVMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKL 360
           GLMPNPETSTSLIAMCDNTQTIAYM+TCGLSAAASTRVSNELGAGN DRAKTAMFATLKL
Sbjct: 301 GLMPNPETSTSLIAMCDNTQTIAYMITCGLSAAASTRVSNELGAGNFDRAKTAMFATLKL 360

Query: 361 SVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGY 420
           SVLLPLL VLALAFG NTWASFF NSIT+MDEFSSM PFLA+SI LDSVQGAISGVARGY
Sbjct: 361 SVLLPLLVVLALAFGHNTWASFFINSITIMDEFSSMVPFLAISIMLDSVQGAISGVARGY 420

Query: 421 GWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNW 480
           GWQHLAVYINLSTFYF+GVTISILLGFK RLYAKGLWIGYICGLSSQT CLL V L   W
Sbjct: 421 GWQHLAVYINLSTFYFIGVTISILLGFKLRLYAKGLWIGYICGLSSQTGCLLLVALSTKW 472

Query: 481 IKMDQSDNEAKDVPFLV 498
           IKMDQSD E KD+P LV
Sbjct: 481 IKMDQSDYEVKDMPLLV 472

BLAST of Spg002701 vs. NCBI nr
Match: XP_022923003.1 (protein DETOXIFICATION 18-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 408/497 (82.09%), Postives = 439/497 (88.33%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           MED REPLLELGF  K+ RWW+R LDMEE KLQL FSLP+ILTNVFYYMI LVSVMFAGH
Sbjct: 1   MEDRREPLLELGFEAKQWRWWKRGLDMEEAKLQLLFSLPLILTNVFYYMISLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
            GELELAAATLA+TLASVTGFAFM                     TGLSGALETLCGQAF
Sbjct: 61  FGELELAAATLASTLASVTGFAFM---------------------TGLSGALETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YGKMGLYLQ SCILSFFCSIIVSVLWIYT+ ILVLLHQE EISR+SA YMKFLIPG
Sbjct: 121 GRKLYGKMGLYLQGSCILSFFCSIIVSVLWIYTERILVLLHQEPEISRISATYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPL+FFSAVPM IH+AIAY LVHWTRLGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLIFFSAVPMLIHVAIAYSLVHWTRLGFNGAPLATSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MSCLILAIYVLKAK+FE+SW+GFS EAF+YSLSSLKLA+PSAAM+CLEYWAVE+MVFLA
Sbjct: 241 WMSCLILAIYVLKAKKFEKSWQGFSSEAFTYSLSSLKLALPSAAMVCLEYWAVEIMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKL 360
           GLMPNPETSTSLIAMCDNT+TIAYM+TCGLSAAASTRVSNELGAGN+DRA+TAMF TLKL
Sbjct: 301 GLMPNPETSTSLIAMCDNTETIAYMITCGLSAAASTRVSNELGAGNLDRARTAMFVTLKL 360

Query: 361 SVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGY 420
           +VL+PLL VLALAFGQ+TWASFFSNS+T+ D FSSM P LA+SITLDSVQGAISGVARGY
Sbjct: 361 AVLVPLLVVLALAFGQSTWASFFSNSVTITDSFSSMVPLLAISITLDSVQGAISGVARGY 420

Query: 421 GWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNW 480
           GWQHLAVYINLSTFYFVGV+ISILLGFK RLYAKGLWIGYICGLSSQTACLL V L    
Sbjct: 421 GWQHLAVYINLSTFYFVGVSISILLGFKLRLYAKGLWIGYICGLSSQTACLLLVALYAKS 476

Query: 481 IKMDQSDNEAKDVPFLV 498
           I++D+SDNE K++  LV
Sbjct: 481 IEIDRSDNEVKNMALLV 476

BLAST of Spg002701 vs. NCBI nr
Match: XP_022984412.1 (protein DETOXIFICATION 18-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 772.7 bits (1994), Expect = 1.9e-219
Identity = 406/497 (81.69%), Postives = 438/497 (88.13%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           MED REPLLELGF  K+ RWW+R LDMEE KLQL FSLP+ILTNVFYYMI LVSVMFAGH
Sbjct: 1   MEDRREPLLELGFEAKQWRWWKRGLDMEEAKLQLLFSLPLILTNVFYYMISLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
           LGELELAAATLA+TLASVTGFAFM                     TGLSGALETLCGQAF
Sbjct: 61  LGELELAAATLASTLASVTGFAFM---------------------TGLSGALETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YGKMGLYLQ SCILSFFCSIIVSVLWIYT+ ILVLLHQE EISR+SA YMKFLIPG
Sbjct: 121 GRKLYGKMGLYLQGSCILSFFCSIIVSVLWIYTERILVLLHQEPEISRISATYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPL+FFSAVPM +H+AI YGLVHWTRLGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLIFFSAVPMLVHLAITYGLVHWTRLGFNGAPLATSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MSCLILAIYVLKAK+FE+SW+GFS EAF+YSLSSLKLA+PSAAM+CLEYWAVE+MVFLA
Sbjct: 241 WMSCLILAIYVLKAKKFEKSWQGFSSEAFTYSLSSLKLALPSAAMVCLEYWAVEIMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKL 360
           GLMPNPETSTSLIAMCDNT+TIAYM+TCGLSA  STRVSNELGAGN+DRA+TAMF TLKL
Sbjct: 301 GLMPNPETSTSLIAMCDNTETIAYMITCGLSATVSTRVSNELGAGNLDRARTAMFVTLKL 360

Query: 361 SVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGY 420
           +VL+PLL VLALAFGQ+TWASFFSNS+T+ D FSSM P LA+SITLDSVQGAISGVARGY
Sbjct: 361 AVLVPLLVVLALAFGQSTWASFFSNSVTITDSFSSMVPLLAISITLDSVQGAISGVARGY 420

Query: 421 GWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNW 480
           GWQHLAVYINLSTFY VGV+ISILLGFK RLY+KGLWIGYICGLSSQTACLL VVL    
Sbjct: 421 GWQHLAVYINLSTFYIVGVSISILLGFKLRLYSKGLWIGYICGLSSQTACLLLVVLYAKS 476

Query: 481 IKMDQSDNEAKDVPFLV 498
           I+MD+SDN+ KD+  LV
Sbjct: 481 IEMDRSDNKVKDIALLV 476

BLAST of Spg002701 vs. NCBI nr
Match: XP_023552679.1 (protein DETOXIFICATION 18-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 771.2 bits (1990), Expect = 5.6e-219
Identity = 409/497 (82.29%), Postives = 437/497 (87.93%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           MED REPLLELGF  K+ RWW+R LDMEE KLQL FSLP+ILTNVFYYMI LVSVMFAGH
Sbjct: 1   MEDRREPLLELGFEAKQWRWWKRGLDMEEAKLQLLFSLPLILTNVFYYMISLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
           LGELELAAATLA+TLASVTGFAFM                     TGLSGALETLCGQAF
Sbjct: 61  LGELELAAATLASTLASVTGFAFM---------------------TGLSGALETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YGKMGLYLQ SC LSFFCSIIVSVLWIYT+ ILVLLHQE EISR SA YMKFLIPG
Sbjct: 121 GRKLYGKMGLYLQGSCTLSFFCSIIVSVLWIYTERILVLLHQEPEISRRSATYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPL+FFSAVPM +H+AIAYGLVHWTRLGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLIFFSAVPMLVHVAIAYGLVHWTRLGFNGAPLATSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MSCLILAIYVLKAK+FE+SW+GFS EAF+YSLSSLKLA+PSAAM+CLEYWAVE+MVFLA
Sbjct: 241 WMSCLILAIYVLKAKKFEKSWQGFSSEAFTYSLSSLKLALPSAAMVCLEYWAVEIMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKL 360
           GLMPNPETSTSLIAMCDNT+TIAYM+TCGLSAAASTRVSNELGAGN+DRA+TAMF TLKL
Sbjct: 301 GLMPNPETSTSLIAMCDNTETIAYMITCGLSAAASTRVSNELGAGNLDRARTAMFVTLKL 360

Query: 361 SVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGY 420
           +VL+PLL VLALAFGQ+TWASFFSNS+T+ D FSSM P LA+SITLDSVQGAISGVARGY
Sbjct: 361 AVLVPLLVVLALAFGQSTWASFFSNSVTITDSFSSMVPLLAISITLDSVQGAISGVARGY 420

Query: 421 GWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNW 480
           GWQHLAVYINLSTFYFVGV+ISILLGFK RLYAKGLWIGYI GLSSQTACLL  VL    
Sbjct: 421 GWQHLAVYINLSTFYFVGVSISILLGFKLRLYAKGLWIGYISGLSSQTACLLFAVLYAKS 476

Query: 481 IKMDQSDNEAKDVPFLV 498
           IKMD SDNE K++  LV
Sbjct: 481 IKMDPSDNEVKNMALLV 476

BLAST of Spg002701 vs. NCBI nr
Match: XP_008456028.1 (PREDICTED: protein DETOXIFICATION 18-like [Cucumis melo])

HSP 1 Score: 769.6 bits (1986), Expect = 1.6e-218
Identity = 411/487 (84.39%), Postives = 432/487 (88.71%), Query Frame = 0

Query: 1   MEDSREPLLEL-GFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAG 60
           MED REPLLEL GF G ++RWW++VLDMEE+KLQL FSLP+ILTNVFYYMI LVSVMFAG
Sbjct: 1   MEDCREPLLELSGFEGNKKRWWKKVLDMEESKLQLLFSLPLILTNVFYYMITLVSVMFAG 60

Query: 61  HLGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQA 120
           HLGELELAAATLANTLASVTGFA M                     TGLSGALETLCGQA
Sbjct: 61  HLGELELAAATLANTLASVTGFALM---------------------TGLSGALETLCGQA 120

Query: 121 FGGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIP 180
           FG KFYGKMGLYLQ SCI SFFCSII+SVLWIYT+ ILVLLHQE EISR+SAMYMKFLIP
Sbjct: 121 FGRKFYGKMGLYLQGSCISSFFCSIILSVLWIYTEQILVLLHQEPEISRISAMYMKFLIP 180

Query: 181 GLFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASIS 240
           GLFAYGLLQNI+RFLQTQSVVMPLVFFSAVPMFIHI IAY LVH TRLGF GAPLAASIS
Sbjct: 181 GLFAYGLLQNIMRFLQTQSVVMPLVFFSAVPMFIHIPIAYALVHCTRLGFSGAPLAASIS 240

Query: 241 LSMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFL 300
           L ++CLILAIYVLKAK+FE+SW GFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFL
Sbjct: 241 LWVACLILAIYVLKAKKFEKSWNGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFL 300

Query: 301 AGLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLK 360
           AGLMPNPETSTSLIAMCDNTQTIAYM+TCGLSAA STRVSNELGAGN D+AKTAMFATLK
Sbjct: 301 AGLMPNPETSTSLIAMCDNTQTIAYMITCGLSAAVSTRVSNELGAGNFDKAKTAMFATLK 360

Query: 361 LSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARG 420
           LSVLLPLL VLALAFG NTW++FF NS T+ DEFSSM PFLA+SITLDSVQGAISGVARG
Sbjct: 361 LSVLLPLLVVLALAFGHNTWSNFFINSNTIRDEFSSMVPFLAISITLDSVQGAISGVARG 420

Query: 421 YGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVN 480
           YGWQHLAVYINLS FYF+GVTISILLGFK RLYAKGLWIGYICGLSSQT CLL V L   
Sbjct: 421 YGWQHLAVYINLSMFYFIGVTISILLGFKLRLYAKGLWIGYICGLSSQTGCLLLVALSAK 466

Query: 481 WIKMDQS 487
           WIKMDQS
Sbjct: 481 WIKMDQS 466

BLAST of Spg002701 vs. ExPASy Swiss-Prot
Match: Q9LUH3 (Protein DETOXIFICATION 18 OS=Arabidopsis thaliana OX=3702 GN=DTX18 PE=2 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 7.3e-137
Identity = 259/472 (54.87%), Postives = 342/472 (72.46%), Query Frame = 0

Query: 12  GFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGHLGELELAAATL 71
           G   K   + ++++D+EE K Q+ +SLPMI TN+FYY IPL SVMFA  LG+LELA ATL
Sbjct: 14  GGRDKSSTFVQKLIDVEEAKTQIIYSLPMIFTNLFYYCIPLTSVMFASQLGQLELAGATL 73

Query: 72  ANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAFGGKFYGKMGLY 131
           AN+ A+VTGFAFM                     TGLSGALETLCGQ FG K Y  +G++
Sbjct: 74  ANSWATVTGFAFM---------------------TGLSGALETLCGQGFGAKSYRMLGIH 133

Query: 132 LQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPGLFAYGLLQNIL 191
           LQ+SCI+S   +I++++LW +T+ + +LL Q+  IS+ +A+YMK+L PGL AYG LQNIL
Sbjct: 134 LQSSCIVSLVFTILITILWFFTESVFLLLRQDPSISKQAALYMKYLAPGLLAYGFLQNIL 193

Query: 192 RFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISLSMSCLILAIYV 251
           RF QTQ +V PLV FS +P+ I+I   Y LVH   LGFIGAP+A SISL ++ + L  YV
Sbjct: 194 RFCQTQCIVTPLVLFSFLPLVINIGTTYALVHLAGLGFIGAPIATSISLWIAFVSLGFYV 253

Query: 252 LKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTS 311
           + + +F+E+W GFS+E+F + + +L L+IPSAAM+CLEYWA E++VFLAGLM NPE +TS
Sbjct: 254 ICSDKFKETWTGFSMESFHHVVLNLTLSIPSAAMVCLEYWAFEILVFLAGLMRNPEITTS 313

Query: 312 LIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKLSVLLPLLAVLA 371
           L+A+C NT++I+YM+TCGLSAA STRVSNELGAGNV  AK A   ++KLS++L L  V+A
Sbjct: 314 LVAICVNTESISYMLTCGLSAATSTRVSNELGAGNVKGAKKATSVSVKLSLVLALGVVIA 373

Query: 372 LAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGYGWQHLAVYINL 431
           +  G + W   FSNS  + + F+S+  FLA SITLDS+QG +SGVARG GWQ LA  INL
Sbjct: 374 ILVGHDAWVGLFSNSHVIKEGFASLRFFLAASITLDSIQGVLSGVARGCGWQRLATVINL 433

Query: 432 STFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNWIKM 484
            TFY +G+ IS+L GFK +L+AKGLWIG ICG+  Q+A LL + +   W K+
Sbjct: 434 GTFYLIGMPISVLCGFKLKLHAKGLWIGLICGMFCQSASLLLMTIFRKWTKL 464

BLAST of Spg002701 vs. ExPASy Swiss-Prot
Match: Q9LUH2 (Protein DETOXIFICATION 19 OS=Arabidopsis thaliana OX=3702 GN=DTX19 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.1e-136
Identity = 264/486 (54.32%), Postives = 348/486 (71.60%), Query Frame = 0

Query: 7   PLLELGFAGKEERWWR--------RVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFA 66
           PLL+    G++ER  R        +V+D+EE K Q+ +SLPMILTNVFYY IP+ SVMFA
Sbjct: 9   PLLDDHVGGEDERGRRSRSSTLVQKVIDVEEAKAQMIYSLPMILTNVFYYCIPITSVMFA 68

Query: 67  GHLGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQ 126
            HLG+LELA ATLAN+ A+V+GFAFM                      GLSG+LETLCGQ
Sbjct: 69  SHLGQLELAGATLANSWATVSGFAFM---------------------VGLSGSLETLCGQ 128

Query: 127 AFGGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLI 186
            FG K Y  +G++LQ+SCI+S   SI++++ W +T+ I  LL Q+  IS+ +A+YMK+  
Sbjct: 129 GFGAKRYRMLGVHLQSSCIVSLVFSILITIFWFFTESIFGLLRQDPSISKQAALYMKYQA 188

Query: 187 PGLFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASI 246
           PGL AYG LQNILRF QTQS++ PLV FS VP+ I+IA AY LV+   LGFIGAP+A SI
Sbjct: 189 PGLLAYGFLQNILRFCQTQSIIAPLVIFSFVPLVINIATAYVLVYVAGLGFIGAPIATSI 248

Query: 247 SLSMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVF 306
           SL ++ L L  YV+ +++F+E+W GFSLE+F Y + +L L++PSAAM+CLEYWA E++VF
Sbjct: 249 SLWIAFLSLGTYVMCSEKFKETWTGFSLESFRYIVINLTLSLPSAAMVCLEYWAFEILVF 308

Query: 307 LAGLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATL 366
           LAG+MPNPE +TSL+A+C NT+ I+YM+T GLSAAASTRVSNELGAGNV  AK A   ++
Sbjct: 309 LAGVMPNPEINTSLVAICVNTEAISYMLTYGLSAAASTRVSNELGAGNVKGAKKATSVSV 368

Query: 367 KLSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVAR 426
           KLS++L L  V+ L  G + W   FS+S  + +EF+S+  FLA SITLDS+QG +SGVAR
Sbjct: 369 KLSLVLALGVVIVLLVGHDGWVGLFSDSYVIKEEFASLRFFLAASITLDSIQGVLSGVAR 428

Query: 427 GYGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCV 485
           G GWQ L   INL+TFY +G+ I+   GFK + YAKGLWIG ICG+  Q++ LL + +  
Sbjct: 429 GCGWQRLVTVINLATFYLIGMPIAAFCGFKLKFYAKGLWIGLICGIFCQSSSLLLMTIFR 473

BLAST of Spg002701 vs. ExPASy Swiss-Prot
Match: Q9C9U1 (Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.4e-87
Identity = 190/464 (40.95%), Postives = 272/464 (58.62%), Query Frame = 0

Query: 28  EETKLQLQFSLPMILTNVFYYMIPLVSVMFAGHLGELELAAATLANTLASVTGFAFMHWD 87
           EE K QL  S P+I  ++  Y + ++SVMF GHLG L L+AA++A + ASVTGF F    
Sbjct: 23  EEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTF---- 82

Query: 88  SVLLFKIKLADLVGHGRLTGLSGALETLCGQAFGGKFYGKMGLYLQASCILSFFCSIIVS 147
                            L G + ALETLCGQA+G K YGK+G+ +Q +  +    S+ +S
Sbjct: 83  -----------------LLGTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLS 142

Query: 148 VLWIYTQPILVLLHQELEISRVSAMYMKFLIPGLFAYGLLQNILRFLQTQSVVMPLVFFS 207
           ++W  T+ ILVL+HQ+  I+ V+  Y K++IP LFAYGLLQ I RFLQ Q+ V P+   S
Sbjct: 143 IIWANTEQILVLVHQDKSIASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCS 202

Query: 208 AVPMFIHIAIAYGLVHWTRLGFIGAPLAASISLSMSCLILAIYVLKAKRFEESWKGFSLE 267
            +   +H+ + +  V  T LG+ GA LA S+S   + ++L+ YV  +     SW GFS E
Sbjct: 203 GITTCLHLLLCWLFVLKTGLGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKE 262

Query: 268 AFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTSLIAMCDNTQTIAYMVT 327
           AF       K+A PSA M+CLE W+ E++V  +GL+PNP   TS++++C NT    + ++
Sbjct: 263 AFQELYDFSKIAFPSAVMVCLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQIS 322

Query: 328 CGLSAAASTRVSNELGAGNVDRAKTAMFATLKLSVLLPLLAVLALAFGQNTWASFFSNSI 387
            GL  AAS RVSNELGAGN   AK A++  + ++V   ++ V  L   +      FS+  
Sbjct: 323 VGLGGAASIRVSNELGAGNPQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDP 382

Query: 388 TMMDEFSSMAPFLAVSITLDSVQGAISGVARGYGWQHLAVYINLSTFYFVGVTISILLGF 447
            ++   +SM P +A    LD +Q  +SGVARG GWQ +   +NL ++Y VGV + +LLGF
Sbjct: 383 KIIAYAASMIPIVACGNFLDGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGF 442

Query: 448 KFRLYAKGLWIGYICGLSSQTACLLSVVLCVNWIKMDQSDNEAK 492
            F +  +GLW+G +  LS Q  CL  V +  NW      D EAK
Sbjct: 443 HFHIGGRGLWLGIVTALSVQVLCLSLVTIFTNW------DKEAK 459

BLAST of Spg002701 vs. ExPASy Swiss-Prot
Match: Q9FHB6 (Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.0e-86
Identity = 193/464 (41.59%), Postives = 275/464 (59.27%), Query Frame = 0

Query: 28  EETKLQLQFSLPMILTNVFYYMIPLVSVMFAGHLGELELAAATLANTLASVTGFAFMHWD 87
           EE K QL  S P+I  ++  + + ++SVMF GHLG L L+AA++A + ASVTGF+F    
Sbjct: 25  EEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFSF---- 84

Query: 88  SVLLFKIKLADLVGHGRLTGLSGALETLCGQAFGGKFYGKMGLYLQASCILSFFCSIIVS 147
                            L G + AL+TLCGQA+G K YG +G+ +Q +  +    SI +S
Sbjct: 85  -----------------LMGTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLS 144

Query: 148 VLWIYTQPILVLLHQELEISRVSAMYMKFLIPGLFAYGLLQNILRFLQTQSVVMPLVFFS 207
           ++W  T+ +LV   Q   I+ ++  Y KF+IP +FAYGLLQ   RFLQ Q+ V P+VF S
Sbjct: 145 IIWANTEHLLVFFGQNKSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCS 204

Query: 208 AVPMFIHIAIAYGLVHWTRLGFIGAPLAASISLSMSCLILAIYVLKAKRFEESWKGFSLE 267
            V   +H+ + + LV  + LGF GA LA SIS  ++ ++L  YV  +     +W GFS E
Sbjct: 205 GVTTSLHVLLCWVLVFKSGLGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKE 264

Query: 268 AFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTSLIAMCDNTQTIAYMVT 327
           A    L  L+LA+PSA M+CLE W+ E++V L+GL+PNP   TS++++C NT    +M+ 
Sbjct: 265 ALRDILPFLRLAVPSALMVCLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIP 324

Query: 328 CGLSAAASTRVSNELGAGNVDRAKTAMFATLKLSVLLPLLAVLALAFGQNTWASFFSNSI 387
            GLS AASTR+SNELGAGN   AK A+   + ++V   ++    L   +N W   +S+ +
Sbjct: 325 FGLSGAASTRISNELGAGNPKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSEL 384

Query: 388 TMMDEFSSMAPFLAVSITLDSVQGAISGVARGYGWQHLAVYINLSTFYFVGVTISILLGF 447
            ++   +SM P LA+   LDS+Q  +SGVARG GWQ +   INL ++Y VGV   +LL F
Sbjct: 385 EVVSYVASMMPILALGNFLDSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAF 444

Query: 448 KFRLYAKGLWIGYICGLSSQTACLLSVVLCVNWIKMDQSDNEAK 492
            F +  +GLW+G IC L  Q   L  V +  NW      D EAK
Sbjct: 445 HFHVGGRGLWLGIICALVVQVFGLGLVTIFTNW------DEEAK 461

BLAST of Spg002701 vs. ExPASy Swiss-Prot
Match: F4IHU9 (Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 7.9e-83
Identity = 188/481 (39.09%), Postives = 279/481 (58.00%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           M + RE +L     G++E+  R V   EE + QL  S P+I  ++  + + ++SVMF GH
Sbjct: 1   MREEREDMLSWPLIGEKEKRSRFV--KEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
           LG L L+AA++A + ASVTGF F                     L G + A++T+CGQ++
Sbjct: 61  LGSLPLSAASIATSFASVTGFTF---------------------LMGTASAMDTVCGQSY 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YG +G+ +Q + ++    S+ +S++W  T+  LV   Q+  I+ +S  Y +F+IP 
Sbjct: 121 GAKMYGMLGIQMQRAMLVLTLLSVPLSIVWANTEHFLVFFGQDKSIAHLSGSYARFMIPS 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           +FAYGLLQ + RFLQ Q+ V+P+V  S V   +H+ I + LV  + LGF GA +A +IS 
Sbjct: 181 IFAYGLLQCLNRFLQAQNNVIPVVICSGVTTSLHVIICWVLVLKSGLGFRGAAVANAISY 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLC-LEYWAVEVMVFL 300
            ++ ++L+ YV  +     +W GFS EA    +  +KL IPSA M+C LE W+ E++V  
Sbjct: 241 WLNVILLSCYVKFSPSCSLTWTGFSKEARRDIIPFMKLVIPSAFMVCSLEMWSFELLVLS 300

Query: 301 AGLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLK 360
           +GL+PNP   TS    C  T    +M+  GLS AASTRVSNELG+GN   AK A+   L 
Sbjct: 301 SGLLPNPVLETS----CPRT---VWMIPFGLSGAASTRVSNELGSGNPKGAKLAVRVVLS 360

Query: 361 LSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARG 420
            S++  +L    L   +  W   +S+   ++   +SM P LA+  +LDS Q  +SGVARG
Sbjct: 361 FSIVESILVGTVLILIRKIWGFAYSSDPEVVSHVASMLPILALGHSLDSFQTVLSGVARG 420

Query: 421 YGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVN 480
            GWQ +  ++NL ++Y VGV   +LLGF F +  +GLW+G IC L  Q  CL  +    N
Sbjct: 421 CGWQKIGAFVNLGSYYLVGVPFGLLLGFHFHVGGRGLWLGIICALIVQGVCLSLITFFTN 451

BLAST of Spg002701 vs. ExPASy TrEMBL
Match: A0A6J1E8D8 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430819 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.4e-220
Identity = 408/497 (82.09%), Postives = 439/497 (88.33%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           MED REPLLELGF  K+ RWW+R LDMEE KLQL FSLP+ILTNVFYYMI LVSVMFAGH
Sbjct: 1   MEDRREPLLELGFEAKQWRWWKRGLDMEEAKLQLLFSLPLILTNVFYYMISLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
            GELELAAATLA+TLASVTGFAFM                     TGLSGALETLCGQAF
Sbjct: 61  FGELELAAATLASTLASVTGFAFM---------------------TGLSGALETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YGKMGLYLQ SCILSFFCSIIVSVLWIYT+ ILVLLHQE EISR+SA YMKFLIPG
Sbjct: 121 GRKLYGKMGLYLQGSCILSFFCSIIVSVLWIYTERILVLLHQEPEISRISATYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPL+FFSAVPM IH+AIAY LVHWTRLGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLIFFSAVPMLIHVAIAYSLVHWTRLGFNGAPLATSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MSCLILAIYVLKAK+FE+SW+GFS EAF+YSLSSLKLA+PSAAM+CLEYWAVE+MVFLA
Sbjct: 241 WMSCLILAIYVLKAKKFEKSWQGFSSEAFTYSLSSLKLALPSAAMVCLEYWAVEIMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKL 360
           GLMPNPETSTSLIAMCDNT+TIAYM+TCGLSAAASTRVSNELGAGN+DRA+TAMF TLKL
Sbjct: 301 GLMPNPETSTSLIAMCDNTETIAYMITCGLSAAASTRVSNELGAGNLDRARTAMFVTLKL 360

Query: 361 SVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGY 420
           +VL+PLL VLALAFGQ+TWASFFSNS+T+ D FSSM P LA+SITLDSVQGAISGVARGY
Sbjct: 361 AVLVPLLVVLALAFGQSTWASFFSNSVTITDSFSSMVPLLAISITLDSVQGAISGVARGY 420

Query: 421 GWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNW 480
           GWQHLAVYINLSTFYFVGV+ISILLGFK RLYAKGLWIGYICGLSSQTACLL V L    
Sbjct: 421 GWQHLAVYINLSTFYFVGVSISILLGFKLRLYAKGLWIGYICGLSSQTACLLLVALYAKS 476

Query: 481 IKMDQSDNEAKDVPFLV 498
           I++D+SDNE K++  LV
Sbjct: 481 IEIDRSDNEVKNMALLV 476

BLAST of Spg002701 vs. ExPASy TrEMBL
Match: A0A6J1J241 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482720 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 9.3e-220
Identity = 406/497 (81.69%), Postives = 438/497 (88.13%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           MED REPLLELGF  K+ RWW+R LDMEE KLQL FSLP+ILTNVFYYMI LVSVMFAGH
Sbjct: 1   MEDRREPLLELGFEAKQWRWWKRGLDMEEAKLQLLFSLPLILTNVFYYMISLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
           LGELELAAATLA+TLASVTGFAFM                     TGLSGALETLCGQAF
Sbjct: 61  LGELELAAATLASTLASVTGFAFM---------------------TGLSGALETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YGKMGLYLQ SCILSFFCSIIVSVLWIYT+ ILVLLHQE EISR+SA YMKFLIPG
Sbjct: 121 GRKLYGKMGLYLQGSCILSFFCSIIVSVLWIYTERILVLLHQEPEISRISATYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPL+FFSAVPM +H+AI YGLVHWTRLGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLIFFSAVPMLVHLAITYGLVHWTRLGFNGAPLATSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MSCLILAIYVLKAK+FE+SW+GFS EAF+YSLSSLKLA+PSAAM+CLEYWAVE+MVFLA
Sbjct: 241 WMSCLILAIYVLKAKKFEKSWQGFSSEAFTYSLSSLKLALPSAAMVCLEYWAVEIMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKL 360
           GLMPNPETSTSLIAMCDNT+TIAYM+TCGLSA  STRVSNELGAGN+DRA+TAMF TLKL
Sbjct: 301 GLMPNPETSTSLIAMCDNTETIAYMITCGLSATVSTRVSNELGAGNLDRARTAMFVTLKL 360

Query: 361 SVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGY 420
           +VL+PLL VLALAFGQ+TWASFFSNS+T+ D FSSM P LA+SITLDSVQGAISGVARGY
Sbjct: 361 AVLVPLLVVLALAFGQSTWASFFSNSVTITDSFSSMVPLLAISITLDSVQGAISGVARGY 420

Query: 421 GWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNW 480
           GWQHLAVYINLSTFY VGV+ISILLGFK RLY+KGLWIGYICGLSSQTACLL VVL    
Sbjct: 421 GWQHLAVYINLSTFYIVGVSISILLGFKLRLYSKGLWIGYICGLSSQTACLLLVVLYAKS 476

Query: 481 IKMDQSDNEAKDVPFLV 498
           I+MD+SDN+ KD+  LV
Sbjct: 481 IEMDRSDNKVKDIALLV 476

BLAST of Spg002701 vs. ExPASy TrEMBL
Match: A0A1S3C2Z1 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103496079 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.9e-219
Identity = 411/487 (84.39%), Postives = 432/487 (88.71%), Query Frame = 0

Query: 1   MEDSREPLLEL-GFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAG 60
           MED REPLLEL GF G ++RWW++VLDMEE+KLQL FSLP+ILTNVFYYMI LVSVMFAG
Sbjct: 1   MEDCREPLLELSGFEGNKKRWWKKVLDMEESKLQLLFSLPLILTNVFYYMITLVSVMFAG 60

Query: 61  HLGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQA 120
           HLGELELAAATLANTLASVTGFA M                     TGLSGALETLCGQA
Sbjct: 61  HLGELELAAATLANTLASVTGFALM---------------------TGLSGALETLCGQA 120

Query: 121 FGGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIP 180
           FG KFYGKMGLYLQ SCI SFFCSII+SVLWIYT+ ILVLLHQE EISR+SAMYMKFLIP
Sbjct: 121 FGRKFYGKMGLYLQGSCISSFFCSIILSVLWIYTEQILVLLHQEPEISRISAMYMKFLIP 180

Query: 181 GLFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASIS 240
           GLFAYGLLQNI+RFLQTQSVVMPLVFFSAVPMFIHI IAY LVH TRLGF GAPLAASIS
Sbjct: 181 GLFAYGLLQNIMRFLQTQSVVMPLVFFSAVPMFIHIPIAYALVHCTRLGFSGAPLAASIS 240

Query: 241 LSMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFL 300
           L ++CLILAIYVLKAK+FE+SW GFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFL
Sbjct: 241 LWVACLILAIYVLKAKKFEKSWNGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFL 300

Query: 301 AGLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLK 360
           AGLMPNPETSTSLIAMCDNTQTIAYM+TCGLSAA STRVSNELGAGN D+AKTAMFATLK
Sbjct: 301 AGLMPNPETSTSLIAMCDNTQTIAYMITCGLSAAVSTRVSNELGAGNFDKAKTAMFATLK 360

Query: 361 LSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARG 420
           LSVLLPLL VLALAFG NTW++FF NS T+ DEFSSM PFLA+SITLDSVQGAISGVARG
Sbjct: 361 LSVLLPLLVVLALAFGHNTWSNFFINSNTIRDEFSSMVPFLAISITLDSVQGAISGVARG 420

Query: 421 YGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVN 480
           YGWQHLAVYINLS FYF+GVTISILLGFK RLYAKGLWIGYICGLSSQT CLL V L   
Sbjct: 421 YGWQHLAVYINLSMFYFIGVTISILLGFKLRLYAKGLWIGYICGLSSQTGCLLLVALSAK 466

Query: 481 WIKMDQS 487
           WIKMDQS
Sbjct: 481 WIKMDQS 466

BLAST of Spg002701 vs. ExPASy TrEMBL
Match: A0A6J1E4Y6 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430819 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.3e-218
Identity = 408/498 (81.93%), Postives = 439/498 (88.15%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           MED REPLLELGF  K+ RWW+R LDMEE KLQL FSLP+ILTNVFYYMI LVSVMFAGH
Sbjct: 1   MEDRREPLLELGFEAKQWRWWKRGLDMEEAKLQLLFSLPLILTNVFYYMISLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
            GELELAAATLA+TLASVTGFAFM                     TGLSGALETLCGQAF
Sbjct: 61  FGELELAAATLASTLASVTGFAFM---------------------TGLSGALETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YGKMGLYLQ SCILSFFCSIIVSVLWIYT+ ILVLLHQE EISR+SA YMKFLIPG
Sbjct: 121 GRKLYGKMGLYLQGSCILSFFCSIIVSVLWIYTERILVLLHQEPEISRISATYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPL+FFSAVPM IH+AIAY LVHWTRLGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLIFFSAVPMLIHVAIAYSLVHWTRLGFNGAPLATSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MSCLILAIYVLKAK+FE+SW+GFS EAF+YSLSSLKLA+PSAAM+CLEYWAVE+MVFLA
Sbjct: 241 WMSCLILAIYVLKAKKFEKSWQGFSSEAFTYSLSSLKLALPSAAMVCLEYWAVEIMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAA-STRVSNELGAGNVDRAKTAMFATLK 360
           GLMPNPETSTSLIAMCDNT+TIAYM+TCGLSAAA STRVSNELGAGN+DRA+TAMF TLK
Sbjct: 301 GLMPNPETSTSLIAMCDNTETIAYMITCGLSAAASSTRVSNELGAGNLDRARTAMFVTLK 360

Query: 361 LSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARG 420
           L+VL+PLL VLALAFGQ+TWASFFSNS+T+ D FSSM P LA+SITLDSVQGAISGVARG
Sbjct: 361 LAVLVPLLVVLALAFGQSTWASFFSNSVTITDSFSSMVPLLAISITLDSVQGAISGVARG 420

Query: 421 YGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVN 480
           YGWQHLAVYINLSTFYFVGV+ISILLGFK RLYAKGLWIGYICGLSSQTACLL V L   
Sbjct: 421 YGWQHLAVYINLSTFYFVGVSISILLGFKLRLYAKGLWIGYICGLSSQTACLLLVALYAK 477

Query: 481 WIKMDQSDNEAKDVPFLV 498
            I++D+SDNE K++  LV
Sbjct: 481 SIEIDRSDNEVKNMALLV 477

BLAST of Spg002701 vs. ExPASy TrEMBL
Match: A0A6J1J556 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482720 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.8e-218
Identity = 406/498 (81.53%), Postives = 439/498 (88.15%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           MED REPLLELGF  K+ RWW+R LDMEE KLQL FSLP+ILTNVFYYMI LVSVMFAGH
Sbjct: 1   MEDRREPLLELGFEAKQWRWWKRGLDMEEAKLQLLFSLPLILTNVFYYMISLVSVMFAGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
           LGELELAAATLA+TLASVTGFAFM                     TGLSGALETLCGQAF
Sbjct: 61  LGELELAAATLASTLASVTGFAFM---------------------TGLSGALETLCGQAF 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YGKMGLYLQ SCILSFFCSIIVSVLWIYT+ ILVLLHQE EISR+SA YMKFLIPG
Sbjct: 121 GRKLYGKMGLYLQGSCILSFFCSIIVSVLWIYTERILVLLHQEPEISRISATYMKFLIPG 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           LFAYGLLQNILRFLQTQSVVMPL+FFSAVPM +H+AI YGLVHWTRLGF GAPLA SISL
Sbjct: 181 LFAYGLLQNILRFLQTQSVVMPLIFFSAVPMLVHLAITYGLVHWTRLGFNGAPLATSISL 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLA 300
            MSCLILAIYVLKAK+FE+SW+GFS EAF+YSLSSLKLA+PSAAM+CLEYWAVE+MVFLA
Sbjct: 241 WMSCLILAIYVLKAKKFEKSWQGFSSEAFTYSLSSLKLALPSAAMVCLEYWAVEIMVFLA 300

Query: 301 GLMPNPETSTSLIAMCDNTQTIAYMVTCGLSA-AASTRVSNELGAGNVDRAKTAMFATLK 360
           GLMPNPETSTSLIAMCDNT+TIAYM+TCGLSA  +STRVSNELGAGN+DRA+TAMF TLK
Sbjct: 301 GLMPNPETSTSLIAMCDNTETIAYMITCGLSATVSSTRVSNELGAGNLDRARTAMFVTLK 360

Query: 361 LSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARG 420
           L+VL+PLL VLALAFGQ+TWASFFSNS+T+ D FSSM P LA+SITLDSVQGAISGVARG
Sbjct: 361 LAVLVPLLVVLALAFGQSTWASFFSNSVTITDSFSSMVPLLAISITLDSVQGAISGVARG 420

Query: 421 YGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVN 480
           YGWQHLAVYINLSTFY VGV+ISILLGFK RLY+KGLWIGYICGLSSQTACLL VVL   
Sbjct: 421 YGWQHLAVYINLSTFYIVGVSISILLGFKLRLYSKGLWIGYICGLSSQTACLLLVVLYAK 477

Query: 481 WIKMDQSDNEAKDVPFLV 498
            I+MD+SDN+ KD+  LV
Sbjct: 481 SIEMDRSDNKVKDIALLV 477

BLAST of Spg002701 vs. TAIR 10
Match: AT3G23550.1 (MATE efflux family protein )

HSP 1 Score: 488.8 bits (1257), Expect = 5.2e-138
Identity = 259/472 (54.87%), Postives = 342/472 (72.46%), Query Frame = 0

Query: 12  GFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGHLGELELAAATL 71
           G   K   + ++++D+EE K Q+ +SLPMI TN+FYY IPL SVMFA  LG+LELA ATL
Sbjct: 14  GGRDKSSTFVQKLIDVEEAKTQIIYSLPMIFTNLFYYCIPLTSVMFASQLGQLELAGATL 73

Query: 72  ANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAFGGKFYGKMGLY 131
           AN+ A+VTGFAFM                     TGLSGALETLCGQ FG K Y  +G++
Sbjct: 74  ANSWATVTGFAFM---------------------TGLSGALETLCGQGFGAKSYRMLGIH 133

Query: 132 LQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPGLFAYGLLQNIL 191
           LQ+SCI+S   +I++++LW +T+ + +LL Q+  IS+ +A+YMK+L PGL AYG LQNIL
Sbjct: 134 LQSSCIVSLVFTILITILWFFTESVFLLLRQDPSISKQAALYMKYLAPGLLAYGFLQNIL 193

Query: 192 RFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISLSMSCLILAIYV 251
           RF QTQ +V PLV FS +P+ I+I   Y LVH   LGFIGAP+A SISL ++ + L  YV
Sbjct: 194 RFCQTQCIVTPLVLFSFLPLVINIGTTYALVHLAGLGFIGAPIATSISLWIAFVSLGFYV 253

Query: 252 LKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTS 311
           + + +F+E+W GFS+E+F + + +L L+IPSAAM+CLEYWA E++VFLAGLM NPE +TS
Sbjct: 254 ICSDKFKETWTGFSMESFHHVVLNLTLSIPSAAMVCLEYWAFEILVFLAGLMRNPEITTS 313

Query: 312 LIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLKLSVLLPLLAVLA 371
           L+A+C NT++I+YM+TCGLSAA STRVSNELGAGNV  AK A   ++KLS++L L  V+A
Sbjct: 314 LVAICVNTESISYMLTCGLSAATSTRVSNELGAGNVKGAKKATSVSVKLSLVLALGVVIA 373

Query: 372 LAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARGYGWQHLAVYINL 431
           +  G + W   FSNS  + + F+S+  FLA SITLDS+QG +SGVARG GWQ LA  INL
Sbjct: 374 ILVGHDAWVGLFSNSHVIKEGFASLRFFLAASITLDSIQGVLSGVARGCGWQRLATVINL 433

Query: 432 STFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVNWIKM 484
            TFY +G+ IS+L GFK +L+AKGLWIG ICG+  Q+A LL + +   W K+
Sbjct: 434 GTFYLIGMPISVLCGFKLKLHAKGLWIGLICGMFCQSASLLLMTIFRKWTKL 464

BLAST of Spg002701 vs. TAIR 10
Match: AT3G23560.1 (MATE efflux family protein )

HSP 1 Score: 487.3 bits (1253), Expect = 1.5e-137
Identity = 264/486 (54.32%), Postives = 348/486 (71.60%), Query Frame = 0

Query: 7   PLLELGFAGKEERWWR--------RVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFA 66
           PLL+    G++ER  R        +V+D+EE K Q+ +SLPMILTNVFYY IP+ SVMFA
Sbjct: 9   PLLDDHVGGEDERGRRSRSSTLVQKVIDVEEAKAQMIYSLPMILTNVFYYCIPITSVMFA 68

Query: 67  GHLGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQ 126
            HLG+LELA ATLAN+ A+V+GFAFM                      GLSG+LETLCGQ
Sbjct: 69  SHLGQLELAGATLANSWATVSGFAFM---------------------VGLSGSLETLCGQ 128

Query: 127 AFGGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLI 186
            FG K Y  +G++LQ+SCI+S   SI++++ W +T+ I  LL Q+  IS+ +A+YMK+  
Sbjct: 129 GFGAKRYRMLGVHLQSSCIVSLVFSILITIFWFFTESIFGLLRQDPSISKQAALYMKYQA 188

Query: 187 PGLFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASI 246
           PGL AYG LQNILRF QTQS++ PLV FS VP+ I+IA AY LV+   LGFIGAP+A SI
Sbjct: 189 PGLLAYGFLQNILRFCQTQSIIAPLVIFSFVPLVINIATAYVLVYVAGLGFIGAPIATSI 248

Query: 247 SLSMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLCLEYWAVEVMVF 306
           SL ++ L L  YV+ +++F+E+W GFSLE+F Y + +L L++PSAAM+CLEYWA E++VF
Sbjct: 249 SLWIAFLSLGTYVMCSEKFKETWTGFSLESFRYIVINLTLSLPSAAMVCLEYWAFEILVF 308

Query: 307 LAGLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATL 366
           LAG+MPNPE +TSL+A+C NT+ I+YM+T GLSAAASTRVSNELGAGNV  AK A   ++
Sbjct: 309 LAGVMPNPEINTSLVAICVNTEAISYMLTYGLSAAASTRVSNELGAGNVKGAKKATSVSV 368

Query: 367 KLSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVAR 426
           KLS++L L  V+ L  G + W   FS+S  + +EF+S+  FLA SITLDS+QG +SGVAR
Sbjct: 369 KLSLVLALGVVIVLLVGHDGWVGLFSDSYVIKEEFASLRFFLAASITLDSIQGVLSGVAR 428

Query: 427 GYGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCV 485
           G GWQ L   INL+TFY +G+ I+   GFK + YAKGLWIG ICG+  Q++ LL + +  
Sbjct: 429 GCGWQRLVTVINLATFYLIGMPIAAFCGFKLKFYAKGLWIGLICGIFCQSSSLLLMTIFR 473

BLAST of Spg002701 vs. TAIR 10
Match: AT1G73700.1 (MATE efflux family protein )

HSP 1 Score: 325.1 bits (832), Expect = 9.9e-89
Identity = 190/464 (40.95%), Postives = 272/464 (58.62%), Query Frame = 0

Query: 28  EETKLQLQFSLPMILTNVFYYMIPLVSVMFAGHLGELELAAATLANTLASVTGFAFMHWD 87
           EE K QL  S P+I  ++  Y + ++SVMF GHLG L L+AA++A + ASVTGF F    
Sbjct: 23  EEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTF---- 82

Query: 88  SVLLFKIKLADLVGHGRLTGLSGALETLCGQAFGGKFYGKMGLYLQASCILSFFCSIIVS 147
                            L G + ALETLCGQA+G K YGK+G+ +Q +  +    S+ +S
Sbjct: 83  -----------------LLGTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLS 142

Query: 148 VLWIYTQPILVLLHQELEISRVSAMYMKFLIPGLFAYGLLQNILRFLQTQSVVMPLVFFS 207
           ++W  T+ ILVL+HQ+  I+ V+  Y K++IP LFAYGLLQ I RFLQ Q+ V P+   S
Sbjct: 143 IIWANTEQILVLVHQDKSIASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCS 202

Query: 208 AVPMFIHIAIAYGLVHWTRLGFIGAPLAASISLSMSCLILAIYVLKAKRFEESWKGFSLE 267
            +   +H+ + +  V  T LG+ GA LA S+S   + ++L+ YV  +     SW GFS E
Sbjct: 203 GITTCLHLLLCWLFVLKTGLGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKE 262

Query: 268 AFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTSLIAMCDNTQTIAYMVT 327
           AF       K+A PSA M+CLE W+ E++V  +GL+PNP   TS++++C NT    + ++
Sbjct: 263 AFQELYDFSKIAFPSAVMVCLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQIS 322

Query: 328 CGLSAAASTRVSNELGAGNVDRAKTAMFATLKLSVLLPLLAVLALAFGQNTWASFFSNSI 387
            GL  AAS RVSNELGAGN   AK A++  + ++V   ++ V  L   +      FS+  
Sbjct: 323 VGLGGAASIRVSNELGAGNPQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDP 382

Query: 388 TMMDEFSSMAPFLAVSITLDSVQGAISGVARGYGWQHLAVYINLSTFYFVGVTISILLGF 447
            ++   +SM P +A    LD +Q  +SGVARG GWQ +   +NL ++Y VGV + +LLGF
Sbjct: 383 KIIAYAASMIPIVACGNFLDGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGF 442

Query: 448 KFRLYAKGLWIGYICGLSSQTACLLSVVLCVNWIKMDQSDNEAK 492
            F +  +GLW+G +  LS Q  CL  V +  NW      D EAK
Sbjct: 443 HFHIGGRGLWLGIVTALSVQVLCLSLVTIFTNW------DKEAK 459

BLAST of Spg002701 vs. TAIR 10
Match: AT5G52450.1 (MATE efflux family protein )

HSP 1 Score: 321.2 bits (822), Expect = 1.4e-87
Identity = 193/464 (41.59%), Postives = 275/464 (59.27%), Query Frame = 0

Query: 28  EETKLQLQFSLPMILTNVFYYMIPLVSVMFAGHLGELELAAATLANTLASVTGFAFMHWD 87
           EE K QL  S P+I  ++  + + ++SVMF GHLG L L+AA++A + ASVTGF+F    
Sbjct: 25  EEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFSF---- 84

Query: 88  SVLLFKIKLADLVGHGRLTGLSGALETLCGQAFGGKFYGKMGLYLQASCILSFFCSIIVS 147
                            L G + AL+TLCGQA+G K YG +G+ +Q +  +    SI +S
Sbjct: 85  -----------------LMGTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLS 144

Query: 148 VLWIYTQPILVLLHQELEISRVSAMYMKFLIPGLFAYGLLQNILRFLQTQSVVMPLVFFS 207
           ++W  T+ +LV   Q   I+ ++  Y KF+IP +FAYGLLQ   RFLQ Q+ V P+VF S
Sbjct: 145 IIWANTEHLLVFFGQNKSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCS 204

Query: 208 AVPMFIHIAIAYGLVHWTRLGFIGAPLAASISLSMSCLILAIYVLKAKRFEESWKGFSLE 267
            V   +H+ + + LV  + LGF GA LA SIS  ++ ++L  YV  +     +W GFS E
Sbjct: 205 GVTTSLHVLLCWVLVFKSGLGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKE 264

Query: 268 AFSYSLSSLKLAIPSAAMLCLEYWAVEVMVFLAGLMPNPETSTSLIAMCDNTQTIAYMVT 327
           A    L  L+LA+PSA M+CLE W+ E++V L+GL+PNP   TS++++C NT    +M+ 
Sbjct: 265 ALRDILPFLRLAVPSALMVCLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIP 324

Query: 328 CGLSAAASTRVSNELGAGNVDRAKTAMFATLKLSVLLPLLAVLALAFGQNTWASFFSNSI 387
            GLS AASTR+SNELGAGN   AK A+   + ++V   ++    L   +N W   +S+ +
Sbjct: 325 FGLSGAASTRISNELGAGNPKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSEL 384

Query: 388 TMMDEFSSMAPFLAVSITLDSVQGAISGVARGYGWQHLAVYINLSTFYFVGVTISILLGF 447
            ++   +SM P LA+   LDS+Q  +SGVARG GWQ +   INL ++Y VGV   +LL F
Sbjct: 385 EVVSYVASMMPILALGNFLDSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAF 444

Query: 448 KFRLYAKGLWIGYICGLSSQTACLLSVVLCVNWIKMDQSDNEAK 492
            F +  +GLW+G IC L  Q   L  V +  NW      D EAK
Sbjct: 445 HFHVGGRGLWLGIICALVVQVFGLGLVTIFTNW------DEEAK 461

BLAST of Spg002701 vs. TAIR 10
Match: AT2G34360.1 (MATE efflux family protein )

HSP 1 Score: 309.3 bits (791), Expect = 5.6e-84
Identity = 188/481 (39.09%), Postives = 279/481 (58.00%), Query Frame = 0

Query: 1   MEDSREPLLELGFAGKEERWWRRVLDMEETKLQLQFSLPMILTNVFYYMIPLVSVMFAGH 60
           M + RE +L     G++E+  R V   EE + QL  S P+I  ++  + + ++SVMF GH
Sbjct: 1   MREEREDMLSWPLIGEKEKRSRFV--KEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGH 60

Query: 61  LGELELAAATLANTLASVTGFAFMHWDSVLLFKIKLADLVGHGRLTGLSGALETLCGQAF 120
           LG L L+AA++A + ASVTGF F                     L G + A++T+CGQ++
Sbjct: 61  LGSLPLSAASIATSFASVTGFTF---------------------LMGTASAMDTVCGQSY 120

Query: 121 GGKFYGKMGLYLQASCILSFFCSIIVSVLWIYTQPILVLLHQELEISRVSAMYMKFLIPG 180
           G K YG +G+ +Q + ++    S+ +S++W  T+  LV   Q+  I+ +S  Y +F+IP 
Sbjct: 121 GAKMYGMLGIQMQRAMLVLTLLSVPLSIVWANTEHFLVFFGQDKSIAHLSGSYARFMIPS 180

Query: 181 LFAYGLLQNILRFLQTQSVVMPLVFFSAVPMFIHIAIAYGLVHWTRLGFIGAPLAASISL 240
           +FAYGLLQ + RFLQ Q+ V+P+V  S V   +H+ I + LV  + LGF GA +A +IS 
Sbjct: 181 IFAYGLLQCLNRFLQAQNNVIPVVICSGVTTSLHVIICWVLVLKSGLGFRGAAVANAISY 240

Query: 241 SMSCLILAIYVLKAKRFEESWKGFSLEAFSYSLSSLKLAIPSAAMLC-LEYWAVEVMVFL 300
            ++ ++L+ YV  +     +W GFS EA    +  +KL IPSA M+C LE W+ E++V  
Sbjct: 241 WLNVILLSCYVKFSPSCSLTWTGFSKEARRDIIPFMKLVIPSAFMVCSLEMWSFELLVLS 300

Query: 301 AGLMPNPETSTSLIAMCDNTQTIAYMVTCGLSAAASTRVSNELGAGNVDRAKTAMFATLK 360
           +GL+PNP   TS    C  T    +M+  GLS AASTRVSNELG+GN   AK A+   L 
Sbjct: 301 SGLLPNPVLETS----CPRT---VWMIPFGLSGAASTRVSNELGSGNPKGAKLAVRVVLS 360

Query: 361 LSVLLPLLAVLALAFGQNTWASFFSNSITMMDEFSSMAPFLAVSITLDSVQGAISGVARG 420
            S++  +L    L   +  W   +S+   ++   +SM P LA+  +LDS Q  +SGVARG
Sbjct: 361 FSIVESILVGTVLILIRKIWGFAYSSDPEVVSHVASMLPILALGHSLDSFQTVLSGVARG 420

Query: 421 YGWQHLAVYINLSTFYFVGVTISILLGFKFRLYAKGLWIGYICGLSSQTACLLSVVLCVN 480
            GWQ +  ++NL ++Y VGV   +LLGF F +  +GLW+G IC L  Q  CL  +    N
Sbjct: 421 CGWQKIGAFVNLGSYYLVGVPFGLLLGFHFHVGGRGLWLGIICALIVQGVCLSLITFFTN 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903838.12.7e-22183.70protein DETOXIFICATION 18-like [Benincasa hispida][more]
XP_022923003.11.1e-21982.09protein DETOXIFICATION 18-like isoform X2 [Cucurbita moschata][more]
XP_022984412.11.9e-21981.69protein DETOXIFICATION 18-like isoform X2 [Cucurbita maxima][more]
XP_023552679.15.6e-21982.29protein DETOXIFICATION 18-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_008456028.11.6e-21884.39PREDICTED: protein DETOXIFICATION 18-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LUH37.3e-13754.87Protein DETOXIFICATION 18 OS=Arabidopsis thaliana OX=3702 GN=DTX18 PE=2 SV=1[more]
Q9LUH22.1e-13654.32Protein DETOXIFICATION 19 OS=Arabidopsis thaliana OX=3702 GN=DTX19 PE=2 SV=1[more]
Q9C9U11.4e-8740.95Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1[more]
Q9FHB62.0e-8641.59Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1[more]
F4IHU97.9e-8339.09Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E8D85.4e-22082.09Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430819 PE=3 SV=1[more]
A0A6J1J2419.3e-22081.69Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482720 PE=3 SV=1[more]
A0A1S3C2Z17.9e-21984.39Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103496079 PE=3 SV=1[more]
A0A6J1E4Y61.3e-21881.93Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430819 PE=3 SV=1[more]
A0A6J1J5561.8e-21881.53Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482720 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G23550.15.2e-13854.87MATE efflux family protein [more]
AT3G23560.11.5e-13754.32MATE efflux family protein [more]
AT1G73700.19.9e-8940.95MATE efflux family protein [more]
AT5G52450.11.4e-8741.59MATE efflux family protein [more]
AT2G34360.15.6e-8439.09MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 39..457
e-value: 3.9E-60
score: 201.8
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 281..441
e-value: 2.8E-23
score: 82.4
coord: 105..219
e-value: 1.4E-19
score: 70.3
coord: 39..84
e-value: 2.0E-6
score: 27.6
NoneNo IPR availablePANTHERPTHR11206:SF310PROTEIN DETOXIFICATIONcoord: 15..491
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 15..491
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 29..483
e-value: 1.14766E-130
score: 384.232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002701.1Spg002701.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity