Spg002699 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002699
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionVID27 domain-containing protein
Locationscaffold6: 4921069 .. 4924100 (+)
RNA-Seq ExpressionSpg002699
SyntenySpg002699
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGCCCTAAAATTTGGCTCCCAAAGCTTTGAATTCTCAAAAAAGTTTGAATCATCGCGATCAAATTTCTTGAAATTCCTCCTGATTTTCTCAAATCCATCCCGAATTTCGATTCTCGTTTTCCCCATTTGCAGATCACACAGACCGAAATGGGTACTGGCCACAGCCGCGAGGATGTTGAACTTTCAGACTCCGATGAATACAATGAAGAAGAGGAAGAGGATGATGAAGAGGAAGAATACGAAGACGCTGAGAAAGAATTGAAGCCGCAATCGACCAAATTGACTGGTACTCCATCTGCAATCGATGATGTTGATGCCAAGCTCAAAGCTCTTAAGCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCTAATTCCAAGAACGCCGTGAAACTTTACCTTCATATCGGTGGAAACACCCCCCGAGCAAAGTGGATCGTCTCTGAGAAACTCACTTTCTACGCGTTTCTCAAGACGGCGAACGTCGACGGAGATAATAACGACGATGAGGAGGAAGATGACGGTGAGGGTTACAAAGGCAATTCGTCGGGTGGGAGAGGCCAGTTACGTTGGGTACTCAAGGTGGGGGCGAAGGTCAGAGCTCTGGTTTCGACAGAAATGCAATTGAAGATGTTTGGCGATCAGCGGCGCGTCGATTTTGTTAACAAAGGCGTGTGGGCATTGAAGTTCCCGAGCGATGAACAGTACCGGAATTTCGTGACTGAATTCCAAGATTGTATGTTTGAGAATGTTTATGGGCTTCAAGCGACTGATGAGAACAAGGTCAAGATTTACGGGAAGGAGTTCATTGGGTGGTTGAAGCCGGAGGTCGCCGACGATTCAATCTGGGAAAACGCCGATATTGAGTTTGGGAAGAGCCCGGGGCCGTCAACTCCGTCAGTGAGGACGAAACAGGACTTGATAGAGGAGTTTGAGGAGGCTGCTAATGGAGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAACGATTCGGGCGTTCAGGTTTACAAGAATCTAAGCCATGGAATTCATGGGAAGGGAGTCTCATTGAAGTTTGGGTCAGGAAATTCTCCAAACATCGGTAGATCAACACCGAAGAAAGGTCTTCTCATGAAGGCTGAGACAAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAGCCCCATGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAAATAGTTACAGAATGGAAGTTTGAAAAGGATGGTACCGATATTACAATGAGGGACATCACAAATGACACCAAAGGGTCGCAATTGGATCCTTCAGAGGCGACATTTTTGGGGTTGGATGATAACAGGCTTTGTCAATGGGACATGAGAGACCGTAGAGGAATGGTTCAGAACATTGGAGCTGCTGATAATTCGCCGGTTCTGAACTGGGCACAGGGGCATCAGTTTTCAAGAGGGACGAACTTCCAATGCTTTGCTACAACAGGGGATGGATCCATTGTTGTTGGATCGGTTGATGGGAAGATTAGGCTGTATTCGAAAACGTCAATGAGACAGGCAAAGACAGCCTTTCCGGGGCTTGGTTCGCCGATTACTCATGTGGATGTTACATATGATGGGAAGTGGATTTTGGGGACGACTGATACTTATTTGATACTCATCTGCACGTTATTCACTGATAAAGATGGCAACACAAAAACTGGGTTCAGTGGTCGTATGGGCAACAAGATTCCAGCTCCGCGGTTGCTAAAGTTGACTCCTCTTGATTCCCATTTGGCTGGAACAGACAATACCTTTCATGGTGGCCACTTTTCTTGGGTAAGAGAAACTTGTTCTCATGTTCTAACTTTGAATACAATACTTGTATCTTGCTCCCTGTTTTGCGGTGGTTTGGTTATTCCTGTTGCTTAAGGTTTTATTATTGTTTTTTTGGCAAAGTATACAATTTCGTTACTAATTGGTTTATTTTTCAGTCAAATTCTTATTGTTTTTGACTAGAACGATGCGTTTTTAATGAGTGGACATTGAATCAAACATTTATCTTCTTCAACTTAGCCAACAAGACTTGAAGGGATGTTGAGGTAGAAAATGTGGACCTATGAATAATGAAGAAGATGATGGTAGCTTTGAAGGGGGTAGAAGAGTGGGTGTCTTTAAAGGCATAATACTTGTTTTGGTTTATTTTCATGTCATGTTGGTTATATTCTATCATCGCTTCTCTTGTTCCTACATTCCCTCATCTCTTAGAAATGGTTTATGTTACTTTTCTGTAGCCTTGTTTGCAAGGTTCATACACACACGTGCTTATGATCTTTTAACGACAATGATTTTCAGGTCACCGAGAGTGGGAAACAAGAGCGTCACCTCGTCGCAACAGTGGGGAAGTTCAGTGTGATATGGGACTTCCATCGGGTGAAGAACAGTGCACATGACTGCTACCGGAATCAACAAGGACTCAAAAGCTGTTACTGCTACAAGATTGTGCTAAAGGATGAATCCATCGTCGAGAGCCGATTCATGCACGACAAGTTTGCTGTCTCCGATTCTCCAGAAGCTCCACTGGTTGTGGCTACCCCCATGAAAGTCAGCTCCATCAGCCTCTCCGGAAAGAGATGAGAAGCTATTGTCGTCGATGGTGTGCCTTTTCATCTCCATTGATTGTTGTGTACTGCTGCTGCTTTCTTTGTTTATCCTCTTCATATGTAATTAGGAATAGTTTTCTTCCTTGTTTCTGGTCATATTTTGTTTGTATGTGCCTTCAAATTGGTTTATTCGTTATCACACTGAAAAATTTGTGTGTTTGAGTGTGTTGTGTAAATAGCTAAATGATGCAAAAGTAGAATTGAGTTTTAGGATTTGTTTGTGCTTCTTGTGTGTTTGCGTGTCTGTAATTTTCAACATGTGAAAGCCCTCCATACTTAGTATCAAACACGACATTGATGAAGATCATCCCACGAATTTGTTGTTTGAAGATAATCTAAGGTTCGGATCATGTTTAGTACTTGAAATTTTAACCTATCTTAAGTTAATAATA

mRNA sequence

ATGGGTACTGGCCACAGCCGCGAGGATGTTGAACTTTCAGACTCCGATGAATACAATGAAGAAGAGGAAGAGGATGATGAAGAGGAAGAATACGAAGACGCTGAGAAAGAATTGAAGCCGCAATCGACCAAATTGACTGGTACTCCATCTGCAATCGATGATGTTGATGCCAAGCTCAAAGCTCTTAAGCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCTAATTCCAAGAACGCCGTGAAACTTTACCTTCATATCGGTGGAAACACCCCCCGAGCAAAGTGGATCGTCTCTGAGAAACTCACTTTCTACGCGTTTCTCAAGACGGCGAACGTCGACGGAGATAATAACGACGATGAGGAGGAAGATGACGGTGAGGGTTACAAAGGCAATTCGTCGGGTGGGAGAGGCCAGTTACGTTGGGTACTCAAGGTGGGGGCGAAGGTCAGAGCTCTGGTTTCGACAGAAATGCAATTGAAGATGTTTGGCGATCAGCGGCGCGTCGATTTTGTTAACAAAGGCGTGTGGGCATTGAAGTTCCCGAGCGATGAACAGTACCGGAATTTCGTGACTGAATTCCAAGATTGTATGTTTGAGAATGTTTATGGGCTTCAAGCGACTGATGAGAACAAGGTCAAGATTTACGGGAAGGAGTTCATTGGGTGGTTGAAGCCGGAGGTCGCCGACGATTCAATCTGGGAAAACGCCGATATTGAGTTTGGGAAGAGCCCGGGGCCGTCAACTCCGTCAGTGAGGACGAAACAGGACTTGATAGAGGAGTTTGAGGAGGCTGCTAATGGAGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAACGATTCGGGCGTTCAGGTTTACAAGAATCTAAGCCATGGAATTCATGGGAAGGGAGTCTCATTGAAGTTTGGGTCAGGAAATTCTCCAAACATCGGTAGATCAACACCGAAGAAAGGTCTTCTCATGAAGGCTGAGACAAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAGCCCCATGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAAATAGTTACAGAATGGAAGTTTGAAAAGGATGGTACCGATATTACAATGAGGGACATCACAAATGACACCAAAGGGTCGCAATTGGATCCTTCAGAGGCGACATTTTTGGGGTTGGATGATAACAGGCTTTGTCAATGGGACATGAGAGACCGTAGAGGAATGGTTCAGAACATTGGAGCTGCTGATAATTCGCCGGTTCTGAACTGGGCACAGGGGCATCAGTTTTCAAGAGGGACGAACTTCCAATGCTTTGCTACAACAGGGGATGGATCCATTGTTGTTGGATCGGTTGATGGGAAGATTAGGCTGTATTCGAAAACGTCAATGAGACAGGCAAAGACAGCCTTTCCGGGGCTTGGTTCGCCGATTACTCATGTGGATGTTACATATGATGGGAAGTGGATTTTGGGGACGACTGATACTTATTTGATACTCATCTGCACGTTATTCACTGATAAAGATGGCAACACAAAAACTGGGTTCAGTGGTCGTATGGGCAACAAGATTCCAGCTCCGCGGTTGCTAAAGTTGACTCCTCTTGATTCCCATTTGGCTGGAACAGACAATACCTTTCATGGTGGCCACTTTTCTTGGGTCACCGAGAGTGGGAAACAAGAGCGTCACCTCGTCGCAACAGTGGGGAAGTTCAGTGTGATATGGGACTTCCATCGGGTGAAGAACAGTGCACATGACTGCTACCGGAATCAACAAGGACTCAAAAGCTGTTACTGCTACAAGATTGTGCTAAAGGATGAATCCATCGTCGAGAGCCGATTCATGCACGACAAGTTTGCTGTCTCCGATTCTCCAGAAGCTCCACTGGTTGTGGCTACCCCCATGAAAGTCAGCTCCATCAGCCTCTCCGGAAAGAGATGA

Coding sequence (CDS)

ATGGGTACTGGCCACAGCCGCGAGGATGTTGAACTTTCAGACTCCGATGAATACAATGAAGAAGAGGAAGAGGATGATGAAGAGGAAGAATACGAAGACGCTGAGAAAGAATTGAAGCCGCAATCGACCAAATTGACTGGTACTCCATCTGCAATCGATGATGTTGATGCCAAGCTCAAAGCTCTTAAGCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCTAATTCCAAGAACGCCGTGAAACTTTACCTTCATATCGGTGGAAACACCCCCCGAGCAAAGTGGATCGTCTCTGAGAAACTCACTTTCTACGCGTTTCTCAAGACGGCGAACGTCGACGGAGATAATAACGACGATGAGGAGGAAGATGACGGTGAGGGTTACAAAGGCAATTCGTCGGGTGGGAGAGGCCAGTTACGTTGGGTACTCAAGGTGGGGGCGAAGGTCAGAGCTCTGGTTTCGACAGAAATGCAATTGAAGATGTTTGGCGATCAGCGGCGCGTCGATTTTGTTAACAAAGGCGTGTGGGCATTGAAGTTCCCGAGCGATGAACAGTACCGGAATTTCGTGACTGAATTCCAAGATTGTATGTTTGAGAATGTTTATGGGCTTCAAGCGACTGATGAGAACAAGGTCAAGATTTACGGGAAGGAGTTCATTGGGTGGTTGAAGCCGGAGGTCGCCGACGATTCAATCTGGGAAAACGCCGATATTGAGTTTGGGAAGAGCCCGGGGCCGTCAACTCCGTCAGTGAGGACGAAACAGGACTTGATAGAGGAGTTTGAGGAGGCTGCTAATGGAGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAACGATTCGGGCGTTCAGGTTTACAAGAATCTAAGCCATGGAATTCATGGGAAGGGAGTCTCATTGAAGTTTGGGTCAGGAAATTCTCCAAACATCGGTAGATCAACACCGAAGAAAGGTCTTCTCATGAAGGCTGAGACAAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAGCCCCATGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAAATAGTTACAGAATGGAAGTTTGAAAAGGATGGTACCGATATTACAATGAGGGACATCACAAATGACACCAAAGGGTCGCAATTGGATCCTTCAGAGGCGACATTTTTGGGGTTGGATGATAACAGGCTTTGTCAATGGGACATGAGAGACCGTAGAGGAATGGTTCAGAACATTGGAGCTGCTGATAATTCGCCGGTTCTGAACTGGGCACAGGGGCATCAGTTTTCAAGAGGGACGAACTTCCAATGCTTTGCTACAACAGGGGATGGATCCATTGTTGTTGGATCGGTTGATGGGAAGATTAGGCTGTATTCGAAAACGTCAATGAGACAGGCAAAGACAGCCTTTCCGGGGCTTGGTTCGCCGATTACTCATGTGGATGTTACATATGATGGGAAGTGGATTTTGGGGACGACTGATACTTATTTGATACTCATCTGCACGTTATTCACTGATAAAGATGGCAACACAAAAACTGGGTTCAGTGGTCGTATGGGCAACAAGATTCCAGCTCCGCGGTTGCTAAAGTTGACTCCTCTTGATTCCCATTTGGCTGGAACAGACAATACCTTTCATGGTGGCCACTTTTCTTGGGTCACCGAGAGTGGGAAACAAGAGCGTCACCTCGTCGCAACAGTGGGGAAGTTCAGTGTGATATGGGACTTCCATCGGGTGAAGAACAGTGCACATGACTGCTACCGGAATCAACAAGGACTCAAAAGCTGTTACTGCTACAAGATTGTGCTAAAGGATGAATCCATCGTCGAGAGCCGATTCATGCACGACAAGTTTGCTGTCTCCGATTCTCCAGAAGCTCCACTGGTTGTGGCTACCCCCATGAAAGTCAGCTCCATCAGCCTCTCCGGAAAGAGATGA

Protein sequence

MGTGHSREDVELSDSDEYNEEEEEDDEEEEYEDAEKELKPQSTKLTGTPSAIDDVDAKLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDNNDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVWALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWENADIEFGKSPGPSTPSVRTKQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNIGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR
Homology
BLAST of Spg002699 vs. NCBI nr
Match: XP_038895945.1 (protein CYPRO4 [Benincasa hispida])

HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 606/657 (92.24%), Postives = 624/657 (94.98%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEE---EDDEEEEYEDAEKELKPQS----TKLTGTPSAID 60
           MGT  SRED +LSDSDEY EEEE   E++EEEEY+DA+KELKPQS     K TGT SAID
Sbjct: 1   MGTAQSREDAKLSDSDEYEEEEEEEVEEEEEEEYKDADKELKPQSISTTAKSTGTSSAID 60

Query: 61  DVDAKLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANV 120
           DVDAKLKALKLKYGSSSPSQ PN KNAVKLYLHIGGNTPRAKWIVSEKLT YAFLKTANV
Sbjct: 61  DVDAKLKALKLKYGSSSPSQTPNFKNAVKLYLHIGGNTPRAKWIVSEKLTSYAFLKTANV 120

Query: 121 DGDNNDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVN 180
           DG N+DDEEED+ EGYK NSS GRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVN
Sbjct: 121 DGGNDDDEEEDNDEGYKANSSAGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVN 180

Query: 181 KGVWALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSI 240
           KGVWALKFP+DE+YRNFVTEFQDC+FENVYGLQATDENKVKIYGKEFIGWLKPEVADDSI
Sbjct: 181 KGVWALKFPTDERYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSI 240

Query: 241 WENADIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSGVQVYK 300
           WENADIEFGKSP   + SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDSGVQVY+
Sbjct: 241 WENADIEFGKSP---SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYR 300

Query: 301 NLSHGIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLD 360
           NLSHGIHG GVSLKFG+G+SPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGL+QLD
Sbjct: 301 NLSHGIHGTGVSLKFGAGHSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLEQLD 360

Query: 361 IETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMV 420
           IETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMV
Sbjct: 361 IETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMV 420

Query: 421 QNIGA-ADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKT 480
           QNIG  ADNS VLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKT
Sbjct: 421 QNIGGPADNSTVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKT 480

Query: 481 AFPGLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRL 540
           AFPGLGSPITHVDVTYDGKWILGT D+YLILICTLFTDKDGNTKTGFSGRMGN+IPAPRL
Sbjct: 481 AFPGLGSPITHVDVTYDGKWILGTADSYLILICTLFTDKDGNTKTGFSGRMGNRIPAPRL 540

Query: 541 LKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYR 600
           LKLTPLDSHLAGTDN FHGGHFSWVTESGKQERHLVATVGKFSVIWDFH VKNSAHDCYR
Sbjct: 541 LKLTPLDSHLAGTDNKFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHHVKNSAHDCYR 600

Query: 601 NQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           NQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 NQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 654

BLAST of Spg002699 vs. NCBI nr
Match: XP_022149230.1 (protein CYPRO4 [Momordica charantia])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 597/652 (91.56%), Postives = 624/652 (95.71%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEEEDDEE-EEYEDAEKELKPQS---TKLTGTPSAIDDVD 60
           MGTG SREDVELSDSDEY EEEEE++E+ +EYEDAEKEL+PQS    K TG P  IDDV+
Sbjct: 1   MGTGQSREDVELSDSDEYKEEEEEEEEDXKEYEDAEKELRPQSISGAKSTGNPPPIDDVE 60

Query: 61  AKLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD 120
           AKL+ALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAF+KTANVDGD
Sbjct: 61  AKLQALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFVKTANVDGD 120

Query: 121 NNDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGV 180
           N++D+EEDD +G K NSS GRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFV+KGV
Sbjct: 121 NDEDDEEDDDDGCKANSSAGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVDKGV 180

Query: 181 WALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWEN 240
           WALKFPSDEQYRNFVT+FQDC+FENVYG++AT+ENKVKIYGKEFIGWLKPEVADDS+WE 
Sbjct: 181 WALKFPSDEQYRNFVTDFQDCLFENVYGIKATEENKVKIYGKEFIGWLKPEVADDSVWEE 240

Query: 241 ADIEFGKSPGPSTPSVRTKQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSH 300
           AD++FGKSP PS P VR KQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSH
Sbjct: 241 ADLDFGKSPEPSAP-VRAKQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSH 300

Query: 301 GIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360
           GIHGKGVSLKF S NSPN  +STPKK LLM+AETNMLLMSPLKEGKPHASGLQQLDIETG
Sbjct: 301 GIHGKGVSLKFNSRNSPNTAQSTPKKALLMRAETNMLLMSPLKEGKPHASGLQQLDIETG 360

Query: 361 KIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNIG 420
           KIVTEWKFEKDGTDITMRDITND KGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMVQNIG
Sbjct: 361 KIVTEWKFEKDGTDITMRDITNDNKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIG 420

Query: 421 AADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGL 480
            ADNSPVLNW QGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGL
Sbjct: 421 -ADNSPVLNWTQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGL 480

Query: 481 GSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLTP 540
           G+PITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGN+IPAPRLLKLTP
Sbjct: 481 GAPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNRIPAPRLLKLTP 540

Query: 541 LDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQGL 600
           LDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFH+VKNSAHDCYRNQQGL
Sbjct: 541 LDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGL 600

Query: 601 KSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           KSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 KSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 650

BLAST of Spg002699 vs. NCBI nr
Match: XP_023552532.1 (protein CYPRO4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 600/653 (91.88%), Postives = 625/653 (95.71%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEEEDDEEEEYEDAEKELKPQ---STKLTGTPSAIDDVDA 60
           MGT  SRED ELSDSDEY EEEEEDDEEEEYEDA++ LKPQ   S K TGT SA+DDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDN 120
           KLKALKLKYGSSSPSQIPN+KNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD 
Sbjct: 61  KLKALKLKYGSSSPSQIPNAKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD- 120

Query: 121 NDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180
           +DD++ED+ +GYK NSSGGRGQLRW+LKVG KVRALVSTEMQLKMFGDQRR+DFVNKGVW
Sbjct: 121 SDDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRIDFVNKGVW 180

Query: 181 ALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWENA 240
           ALKFPSDEQYR+FVT+FQD +FENVYGL+AT+ENKVKIYGKEFIGWLKPEVADDSIWENA
Sbjct: 181 ALKFPSDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENA 240

Query: 241 DIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSGVQVYKNLSH 300
           DIEFGKSP   + SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDSGVQVY+NLSH
Sbjct: 241 DIEFGKSP---SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSH 300

Query: 301 GIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360
           GIHGKG+SLKFG+GNSPN  RSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG
Sbjct: 301 GIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360

Query: 361 KIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNI- 420
           K+VTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMVQNI 
Sbjct: 361 KVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIG 420

Query: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480
           GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG
Sbjct: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480

Query: 481 LGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLT 540
           LGSPITHVDVT+DGKWILGTTDTYLILIC LF DKDGNTKTGFSGRMGNKIPAPRLLKLT
Sbjct: 481 LGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLT 540

Query: 541 PLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQG 600
           PLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNSAHDCYRNQQG
Sbjct: 541 PLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQG 600

Query: 601 LKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           LKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 LKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Spg002699 vs. NCBI nr
Match: XP_022984689.1 (protein CYPRO4-like [Cucurbita maxima])

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 601/653 (92.04%), Postives = 624/653 (95.56%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEEEDDEEEEYEDAEKELKPQ---STKLTGTPSAIDDVDA 60
           MGT  SRED ELSDSDEY EEEEEDDEEEEYEDA++ LKPQ   S K TGT SA+DDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDN 120
           KLKALKLKYGSSSPSQIPN+ NAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD 
Sbjct: 61  KLKALKLKYGSSSPSQIPNASNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD- 120

Query: 121 NDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180
           +DD++EDD + YK NSSGGRGQLRW+LKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW
Sbjct: 121 SDDDQEDDYDAYKANSSGGRGQLRWILKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180

Query: 181 ALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWENA 240
           ALKFPSDEQYR+FVT+FQD +FENVYGL+AT+ENKVKIYGKEFIGWLKPEVADDSIWENA
Sbjct: 181 ALKFPSDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENA 240

Query: 241 DIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSGVQVYKNLSH 300
           DIEFGKSP   + SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDSGVQVY+NLSH
Sbjct: 241 DIEFGKSP---SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSH 300

Query: 301 GIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360
           GIHGKG+SLKFG+GNSPN  RSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG
Sbjct: 301 GIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360

Query: 361 KIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNI- 420
           K+VTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMVQNI 
Sbjct: 361 KVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIG 420

Query: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480
           GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG
Sbjct: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480

Query: 481 LGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLT 540
           LGSPITHVDVT+DGKWILGTTDTYLILIC LF DKDGNTKTGFSGRMGNKIPAPRLLKLT
Sbjct: 481 LGSPITHVDVTHDGKWILGTTDTYLILICVLFIDKDGNTKTGFSGRMGNKIPAPRLLKLT 540

Query: 541 PLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQG 600
           PLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNSAHDCYRNQQG
Sbjct: 541 PLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQG 600

Query: 601 LKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           LKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 LKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Spg002699 vs. NCBI nr
Match: KAG6576955.1 (Protein CYPRO4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 598/653 (91.58%), Postives = 624/653 (95.56%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEEEDDEEEEYEDAEKELKPQ---STKLTGTPSAIDDVDA 60
           MGT  SRED ELSDSDEY EEEEEDDEEEEYEDA++ LKPQ   S K TGT SA+DDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDN 120
           KLKALKLKYGSSSPSQIPN+ NAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKT+NVDGD 
Sbjct: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGD- 120

Query: 121 NDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180
           +DD++ED+ +GYK NSSGGRGQLRW+LKVG KVRALVSTEMQLKMFGDQRRVDFVNKGVW
Sbjct: 121 SDDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180

Query: 181 ALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWENA 240
           ALKFP+DEQYR+FVT+FQD +FENVYGL+AT+ENKVKIYGKEFIGWLKPEVADDSIWENA
Sbjct: 181 ALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENA 240

Query: 241 DIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSGVQVYKNLSH 300
           DIEFGKSP   + SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDSGVQVY+NLSH
Sbjct: 241 DIEFGKSP---SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSH 300

Query: 301 GIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360
           GIHGKG+SLKFG+GNSPN  RSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG
Sbjct: 301 GIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360

Query: 361 KIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNI- 420
           K+VTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMVQNI 
Sbjct: 361 KVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIG 420

Query: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480
           GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG
Sbjct: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480

Query: 481 LGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLT 540
           LGSPITHVDVT+DGKWILGTTDTYLILIC LF DKDGNTKTGFSGRMGNKIPAPRLLKLT
Sbjct: 481 LGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLT 540

Query: 541 PLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQG 600
           PLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNSAHDCYRNQQG
Sbjct: 541 PLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQG 600

Query: 601 LKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           LKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 LKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Spg002699 vs. ExPASy Swiss-Prot
Match: P40781 (Protein CYPRO4 OS=Cynara cardunculus OX=4265 GN=CYPRO4 PE=2 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 4.3e-230
Identity = 389/494 (78.74%), Postives = 428/494 (86.64%), Query Frame = 0

Query: 162 MFGDQRRVDFVNKGVWALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFI 221
           MFGDQRRVDFV+ GVWALKF  DE YR FVT FQ C+FENVYGL+A+DENKVK+YGK+FI
Sbjct: 1   MFGDQRRVDFVDSGVWALKFMRDEDYREFVTRFQSCLFENVYGLKASDENKVKVYGKDFI 60

Query: 222 GWLKPEVADDSIWENADIEFGKSPGPSTPSVRTKQDLIEEFEEAAN--GVQSLTLGALDN 281
           GW+KP+VADDS+WE  D E  +SP   TP VR   DL+EEFEEAA+  G+QS+ LGALDN
Sbjct: 61  GWVKPDVADDSMWEKDDDELWRSPSKMTP-VRGSNDLLEEFEEAASDGGIQSVALGALDN 120

Query: 282 SFLVNDSGVQVYKNLSHGIHGKGVSLKF-------GSGNSPNIGRSTPKKGLLMKAETNM 341
           SFLVNDSGVQV KN SHGIHGKGV +KF       G  +S      TP+K LLM+ ETNM
Sbjct: 121 SFLVNDSGVQVVKNFSHGIHGKGVYVKFDNGGKRVGGSSSGGYSNLTPQKALLMRGETNM 180

Query: 342 LLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATF 401
           LLMSP K GKPH++G+ QLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TF
Sbjct: 181 LLMSPAKAGKPHSTGVNQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTF 240

Query: 402 LGLDDNRLCQWDMRDRRGMVQNIGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVG 461
           LGLDDNRL QWDMRDRRGMVQNI  +D SPVL+W QGHQFSRGTNFQ FATTGDGSIVVG
Sbjct: 241 LGLDDNRLSQWDMRDRRGMVQNIAHSD-SPVLHWTQGHQFSRGTNFQSFATTGDGSIVVG 300

Query: 462 SVDGKIRLYSKTSMRQAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDG 521
           S+DGKIRLYS TSMR AKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILIC+LFTDKDG
Sbjct: 301 SLDGKIRLYSTTSMRMAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICSLFTDKDG 360

Query: 522 NTKTGFSGRMGNKIPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGK 581
            TKTGFSGRMGNKIPAPRLLKLTP+DSH AG +N FHGG FSWVTESGKQERHLVATVGK
Sbjct: 361 KTKTGFSGRMGNKIPAPRLLKLTPVDSHTAGVNNKFHGGRFSWVTESGKQERHLVATVGK 420

Query: 582 FSVIWDFHRVKNSAHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKF-AVSDSPEAPL 641
           FSVIWDF RVKNS H+CYRNQ+GLKSCYCYK++ KDESI+ES FM+DK+ AV DSPEAPL
Sbjct: 421 FSVIWDFQRVKNSGHECYRNQEGLKSCYCYKLMTKDESIIESLFMNDKYAAVGDSPEAPL 480

Query: 642 VVATPMKVSSISLS 646
           V+ATP K++S S+S
Sbjct: 481 VMATPKKITSFSMS 492

BLAST of Spg002699 vs. ExPASy Swiss-Prot
Match: Q555V7 (VID27-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274813 PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 2.5e-28
Identity = 95/360 (26.39%), Postives = 162/360 (45.00%), Query Frame = 0

Query: 255 KQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHGKGVSLKFGSGNSPN 314
           K+ + ++F ++ N   SL +G  D S++V  S + V+     GI            +  N
Sbjct: 579 KKQISKDFSKSKN--SSLIVGYKDKSYVVRGSTIGVFNTDDDGIKFNTAITNIKQKDGKN 638

Query: 315 IGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGTDITMR 374
               +PKK +L + +  +L+++P K+     S + ++D+    IV EW            
Sbjct: 639 F---SPKKMMLQQQDQTLLMLNPEKK-----SNVYKMDLNRPDIVEEWDMNWRNQPTLTN 698

Query: 375 DITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNIGAADNSPVLNWAQGHQFSR 434
            + +  K    + +   F+G + N +   D R+ +  V                  +F  
Sbjct: 699 SVVSQYKNDD-ETNNQLFVGYNGNNMFLVDPRESKNKVT----------------VKFHG 758

Query: 435 GTN----FQCFATTGDGSIVVGSVDGKIRLYSKTSMRQ----------------AKTAFP 494
           G+N      C ATTG G I  G+  G+I+L+SKT   Q                ++T  P
Sbjct: 759 GSNPKSVTTCAATTGSGQIAFGTSKGEIKLFSKTQFDQNKRSVTSSDPLGAIARSRTTLP 818

Query: 495 GLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKL 554
           G+G PI  +DVT DGKWI+ T   Y+++I      KDG+  +GF  R+G + P+P+ L L
Sbjct: 819 GIGDPIVGIDVTKDGKWIVATCKQYIMVIPASL--KDGS--SGFEDRLGARRPSPKRLIL 878

Query: 555 TPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQ 595
            P D    G    F    F+ V +  + E  ++ + G F + W+F ++K +  D Y+ +Q
Sbjct: 879 KPQDIKRMGGVVNFTPAKFNIVGDE-QSETSILTSTGSFLITWNFRKIKQNILDVYQIKQ 906

BLAST of Spg002699 vs. ExPASy Swiss-Prot
Match: Q1MTR3 (Vacuolar import and degradation protein 27 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vid27 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.6e-27
Identity = 126/467 (26.98%), Postives = 214/467 (45.82%), Query Frame = 0

Query: 142 WVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVWALKFPSDEQYRNFVTEFQDCMFEN 201
           W+L+  ++VR     E+ ++   +      +N+  W LK   D++ R++V E     F  
Sbjct: 326 WLLRFDSQVRMEQFQELLMRALWES-----LNQQRW-LKI--DDEQRDYVME----TFHE 385

Query: 202 VYGLQATDENKVKIYGKEFIGWLKPEVADD---SIWENADIEFGKSPGPSTPSVRTKQDL 261
              L+  D    +   ++ +   + E  +D   S +E++  +F           R +++ 
Sbjct: 386 DEELE--DSEDEEFARQQLLSRKEEEEEEDEEASDFEDSFADFSDGEADDLDESRWRKEA 445

Query: 262 IEEFEE-AANGVQSLTLGALDNSFLVNDSGVQVYKNLSHGIHGKGVSLKFGSGN--SPNI 321
            +E     A G ++      D S++V ++ + V+K++      KG+  +    N  +P  
Sbjct: 446 AKEHNSLLAVGYKN------DRSYVVRNNKIGVFKHVDE----KGLKFQTALNNLSTPKG 505

Query: 322 GRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGTDITMRD 381
               P K +L   ++++L  +   E  PH+  L  +DIE GKIV EWK      D+ +  
Sbjct: 506 KSLRPSKLMLHNQDSSILFQT---ENAPHS--LYHMDIEYGKIVDEWKVH---DDVPLVT 565

Query: 382 ITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNIGAADNSPVLNWAQGHQFSRG 441
            T D K +Q+  +E T +GL +N + + D R             N  V    Q  Q++  
Sbjct: 566 FTPDNKFAQM-TAEQTLIGLSNNSIFRIDPR----------VEGNKLVAE--QFKQYATK 625

Query: 442 TNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSPITHVDVTYDGKWILGT 501
            +F   ATT +G I V S  G IRL+ +  +  AKTA P LG  I  VDVT  G ++L T
Sbjct: 626 NDFSSAATTENGYIAVASNKGDIRLFDRIGV-NAKTALPALGEAIIGVDVTASGDFVLAT 685

Query: 502 TDTYLILICTLFTDKDGNTKTGFSGRMG-------NKIPAPRLLKLTPLDSHLAGTDNTF 561
             TY++LI T    K+G     ++GR+G       +K P P+ L+L+P   H+A      
Sbjct: 686 CKTYILLIDTRI--KEGR----YAGRLGFERNFAKDKKPKPKRLQLSP--QHIAMMQREL 738

Query: 562 HGG----HFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYR 592
            GG       + T    +E  +V+++G F + W+  RVK    D Y+
Sbjct: 746 KGGASFTPAKFNTGIDAKETTIVSSIGPFLISWNLDRVKRGFTDSYK 738

BLAST of Spg002699 vs. ExPASy Swiss-Prot
Match: P40157 (Vacuolar import and degradation protein 27 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VID27 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 8.9e-18
Identity = 102/373 (27.35%), Postives = 157/373 (42.09%), Query Frame = 0

Query: 260 EEFEE----AANGVQSLTLGALDN-SFLVNDSGVQVYKNLSHGIHGKGVSLKFGSG--NS 319
           E FEE     + G  SLT+   +N S++  D+ + V+K        +  SL+F +   N 
Sbjct: 407 EAFEEPRRATSKGNSSLTVAFRNNRSYVTRDNRIGVFKT-----DDEDDSLEFVAAIKNI 466

Query: 320 PNIGRST--PKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKIVTEWKFEKDGTD 379
            N+G  +  P K +L   + N++L     E K     L ++DIE GK++ EW       D
Sbjct: 467 SNLGGKSIDPHKPMLYMEDRNLILTDGENENK-----LYKMDIERGKVIEEW----STGD 526

Query: 380 ITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNIGAADNSPVLNWAQGH 439
             +       K  Q+ P E T +G+    + + D R      +N  A D S         
Sbjct: 527 KNVVQYGPTKKFDQMTP-EQTIVGVSQKGVFKIDPRING---KNKIAVDES--------K 586

Query: 440 QFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSPITHVDVTYDGK 499
            +    NF    TT  G I +GS  G I+LY +  +R AKTA P LG  I  +  + DGK
Sbjct: 587 DYVGKYNFSSIGTTESGYIAIGSEKGDIKLYDRLGIR-AKTAIPSLGQAIKFITTSADGK 646

Query: 500 WILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPR-----LLKLTPLDSH--LAG 559
           W+L T ++ L+L+     D       GF        PA       +LK+ P  S   L  
Sbjct: 647 WLLATCESTLLLMDLKIKDGKNAGNIGFL----KSFPASENVKTYVLKIRPEHSASILTY 706

Query: 560 TDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQGLKSCYCYK 617
           T          + T  G+QE+ +V + G +++ W    +         NQ G  + Y Y+
Sbjct: 707 TKKPIRFTKAYFNTGIGQQEQTIVTSTGPYAISWSLKGI--------LNQDGSNN-YPYR 739

BLAST of Spg002699 vs. ExPASy TrEMBL
Match: A0A6J1D560 (protein CYPRO4 OS=Momordica charantia OX=3673 GN=LOC111017701 PE=4 SV=1)

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 597/652 (91.56%), Postives = 624/652 (95.71%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEEEDDEE-EEYEDAEKELKPQS---TKLTGTPSAIDDVD 60
           MGTG SREDVELSDSDEY EEEEE++E+ +EYEDAEKEL+PQS    K TG P  IDDV+
Sbjct: 1   MGTGQSREDVELSDSDEYKEEEEEEEEDXKEYEDAEKELRPQSISGAKSTGNPPPIDDVE 60

Query: 61  AKLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD 120
           AKL+ALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAF+KTANVDGD
Sbjct: 61  AKLQALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFVKTANVDGD 120

Query: 121 NNDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGV 180
           N++D+EEDD +G K NSS GRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFV+KGV
Sbjct: 121 NDEDDEEDDDDGCKANSSAGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVDKGV 180

Query: 181 WALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWEN 240
           WALKFPSDEQYRNFVT+FQDC+FENVYG++AT+ENKVKIYGKEFIGWLKPEVADDS+WE 
Sbjct: 181 WALKFPSDEQYRNFVTDFQDCLFENVYGIKATEENKVKIYGKEFIGWLKPEVADDSVWEE 240

Query: 241 ADIEFGKSPGPSTPSVRTKQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSH 300
           AD++FGKSP PS P VR KQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSH
Sbjct: 241 ADLDFGKSPEPSAP-VRAKQDLIEEFEEAANGVQSLTLGALDNSFLVNDSGVQVYKNLSH 300

Query: 301 GIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360
           GIHGKGVSLKF S NSPN  +STPKK LLM+AETNMLLMSPLKEGKPHASGLQQLDIETG
Sbjct: 301 GIHGKGVSLKFNSRNSPNTAQSTPKKALLMRAETNMLLMSPLKEGKPHASGLQQLDIETG 360

Query: 361 KIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNIG 420
           KIVTEWKFEKDGTDITMRDITND KGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMVQNIG
Sbjct: 361 KIVTEWKFEKDGTDITMRDITNDNKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIG 420

Query: 421 AADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGL 480
            ADNSPVLNW QGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGL
Sbjct: 421 -ADNSPVLNWTQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGL 480

Query: 481 GSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLTP 540
           G+PITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGN+IPAPRLLKLTP
Sbjct: 481 GAPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNRIPAPRLLKLTP 540

Query: 541 LDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQGL 600
           LDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFH+VKNSAHDCYRNQQGL
Sbjct: 541 LDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGL 600

Query: 601 KSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           KSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 KSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 650

BLAST of Spg002699 vs. ExPASy TrEMBL
Match: A0A6J1JB96 (protein CYPRO4-like OS=Cucurbita maxima OX=3661 GN=LOC111482893 PE=4 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 601/653 (92.04%), Postives = 624/653 (95.56%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEEEDDEEEEYEDAEKELKPQ---STKLTGTPSAIDDVDA 60
           MGT  SRED ELSDSDEY EEEEEDDEEEEYEDA++ LKPQ   S K TGT SA+DDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDN 120
           KLKALKLKYGSSSPSQIPN+ NAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD 
Sbjct: 61  KLKALKLKYGSSSPSQIPNASNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGD- 120

Query: 121 NDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180
           +DD++EDD + YK NSSGGRGQLRW+LKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW
Sbjct: 121 SDDDQEDDYDAYKANSSGGRGQLRWILKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180

Query: 181 ALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWENA 240
           ALKFPSDEQYR+FVT+FQD +FENVYGL+AT+ENKVKIYGKEFIGWLKPEVADDSIWENA
Sbjct: 181 ALKFPSDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENA 240

Query: 241 DIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSGVQVYKNLSH 300
           DIEFGKSP   + SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDSGVQVY+NLSH
Sbjct: 241 DIEFGKSP---SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSH 300

Query: 301 GIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360
           GIHGKG+SLKFG+GNSPN  RSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG
Sbjct: 301 GIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360

Query: 361 KIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNI- 420
           K+VTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMVQNI 
Sbjct: 361 KVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIG 420

Query: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480
           GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG
Sbjct: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480

Query: 481 LGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLT 540
           LGSPITHVDVT+DGKWILGTTDTYLILIC LF DKDGNTKTGFSGRMGNKIPAPRLLKLT
Sbjct: 481 LGSPITHVDVTHDGKWILGTTDTYLILICVLFIDKDGNTKTGFSGRMGNKIPAPRLLKLT 540

Query: 541 PLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQG 600
           PLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNSAHDCYRNQQG
Sbjct: 541 PLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQG 600

Query: 601 LKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           LKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 LKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Spg002699 vs. ExPASy TrEMBL
Match: A0A6J1E9T2 (protein CYPRO4-like OS=Cucurbita moschata OX=3662 GN=LOC111430675 PE=4 SV=1)

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 598/653 (91.58%), Postives = 623/653 (95.41%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEEEDDEEEEYEDAEKELKPQ---STKLTGTPSAIDDVDA 60
           MGT  SRED ELSDSDEY EEEEEDDEEEEYEDA++ LKPQ   S K TGT SA+DDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDN 120
           KLKALKLKYGSSSPSQIPN+ NAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKT+NVDGD 
Sbjct: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGD- 120

Query: 121 NDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180
           +DD++ED+ +GYK NSSGGRGQLRW+LKVG KVRALVSTEMQLKMFGDQRRVDFVNKGVW
Sbjct: 121 SDDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVW 180

Query: 181 ALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADDSIWENA 240
           ALKFP+DEQYR+FVT+FQD +FENVYGL+AT+ENKVKIYGKEFIGWLKPEVADDSIWENA
Sbjct: 181 ALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENA 240

Query: 241 DIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSGVQVYKNLSH 300
           DIEFGKSPG    SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDSGVQVY+NLSH
Sbjct: 241 DIEFGKSPG---SSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSH 300

Query: 301 GIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETG 360
           GIHGKG+SLKFG+GNSPN  RSTPKKGLL KAETNMLLMSPLKEGKPHASGLQQLDIETG
Sbjct: 301 GIHGKGISLKFGAGNSPNSSRSTPKKGLLTKAETNMLLMSPLKEGKPHASGLQQLDIETG 360

Query: 361 KIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRGMVQNI- 420
           K+VTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMRDRRGMVQNI 
Sbjct: 361 KVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIG 420

Query: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480
           GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG
Sbjct: 421 GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPG 480

Query: 481 LGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAPRLLKLT 540
           LGSPITHVDVT+DGKWILGTTDTYLILIC LF DKDGNTKTGFSGRMGNKIPAPRLLKLT
Sbjct: 481 LGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLT 540

Query: 541 PLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDCYRNQQG 600
           PLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNSAHDCYRNQQG
Sbjct: 541 PLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQG 600

Query: 601 LKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           LKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 LKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Spg002699 vs. ExPASy TrEMBL
Match: A0A0A0LAA1 (VID27 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G207380 PE=4 SV=1)

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 601/659 (91.20%), Postives = 624/659 (94.69%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEE-EDDEEEEYEDAEKELKPQS----TKLTGTPSAIDDV 60
           MG+G SREDVELSDSD+Y EEEE +DD+EEEYEDA+KELKP S     K TGT SAIDDV
Sbjct: 1   MGSGQSREDVELSDSDDYKEEEEDDDDDEEEYEDADKELKPHSISTTAKSTGTSSAIDDV 60

Query: 61  DAKLKALKLKYGSS----SPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTA 120
           DAKLKALKLKYGSS    SPSQ PNSKNAVKLYLHIGGNTPRAKWIVSEK TFY FLKTA
Sbjct: 61  DAKLKALKLKYGSSSSSPSPSQTPNSKNAVKLYLHIGGNTPRAKWIVSEKFTFYVFLKTA 120

Query: 121 NVDGDNNDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRRVDF 180
           NVDG N+DDEEEDD EG+  NSSGGR   RWVLKVGAKVRALVSTEMQLKMFG+QRRVDF
Sbjct: 121 NVDGHNDDDEEEDDDEGHNANSSGGR---RWVLKVGAKVRALVSTEMQLKMFGEQRRVDF 180

Query: 181 VNKGVWALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEVADD 240
           VNKGVWALKFPS EQYRNFVTEFQDC+FENVYGLQATDENKVKIYGKEFIGWLKPEVADD
Sbjct: 181 VNKGVWALKFPSGEQYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLKPEVADD 240

Query: 241 SIWENADIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSGVQV 300
           SIWENADI+F KSP   + SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDSGVQV
Sbjct: 241 SIWENADIDFEKSP---SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQV 300

Query: 301 YKNLSHGIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQ 360
           Y+NLSHGIHGKGVSLKFG+G+SPNIGRSTPKKGLL+KAETNMLLMSPLKEGKPH +GL+Q
Sbjct: 301 YRNLSHGIHGKGVSLKFGAGHSPNIGRSTPKKGLLVKAETNMLLMSPLKEGKPHTTGLEQ 360

Query: 361 LDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMRDRRG 420
           LDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMRDRRG
Sbjct: 361 LDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRG 420

Query: 421 MVQNI-GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQA 480
           MVQNI G+ADNS VLNWAQGHQFSRGTNFQCFATTGDGSIVVGS+DGKIRLYSKTSMRQA
Sbjct: 421 MVQNISGSADNSMVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSIDGKIRLYSKTSMRQA 480

Query: 481 KTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNKIPAP 540
           KTAFPGLGSPITHVDVTYDGKWILGTTD+YLILICTLFTDKDGNTKTGFSGRMGN+IPAP
Sbjct: 481 KTAFPGLGSPITHVDVTYDGKWILGTTDSYLILICTLFTDKDGNTKTGFSGRMGNRIPAP 540

Query: 541 RLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSAHDC 600
           RLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNS+HDC
Sbjct: 541 RLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSSHDC 600

Query: 601 YRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 653

BLAST of Spg002699 vs. ExPASy TrEMBL
Match: A0A5D3DQK8 (Protein CYPRO4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1441G00020 PE=4 SV=1)

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 602/663 (90.80%), Postives = 625/663 (94.27%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSDEYNEEEE---EDDEEEEYEDAEKELKPQS----TKLTGTPSAID 60
           MG+G SREDVELSDSD+Y EEEE   +DD+EEEY+DA+KELKP S     K TGT SAID
Sbjct: 1   MGSGQSREDVELSDSDDYKEEEEDDDDDDDEEEYQDADKELKPHSVSTTAKSTGTSSAID 60

Query: 61  DVDAKLKALKLKYGSS------SPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAF 120
           DVDAKLKALKLKYGSS      SPS+ PNSKNAVKLYLHIGGNTPRAKWIVSEKL+FYAF
Sbjct: 61  DVDAKLKALKLKYGSSSSSPSPSPSKTPNSKNAVKLYLHIGGNTPRAKWIVSEKLSFYAF 120

Query: 121 LKTANVDGDNNDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQR 180
           LKTANVDG N+DDEEEDD +GY  NSSGGR   RWVLKVGAKVRALVSTEMQLKMFGDQR
Sbjct: 121 LKTANVDGHNDDDEEEDDDQGYNANSSGGR---RWVLKVGAKVRALVSTEMQLKMFGDQR 180

Query: 181 RVDFVNKGVWALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPE 240
           RVDFVNKGVWALKFPS EQYRNFVTEFQDC+FENVYGLQATDENKVKIYGKEFIGWLKPE
Sbjct: 181 RVDFVNKGVWALKFPSGEQYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLKPE 240

Query: 241 VADDSIWENADIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDS 300
           VADDSIWENADIEF KSP   + SVRTKQDLIEEFEEAAN GVQSLTLGALDNSFLVNDS
Sbjct: 241 VADDSIWENADIEFEKSP---SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDS 300

Query: 301 GVQVYKNLSHGIHGKGVSLKFGSGNSPNIGRSTPKKGLLMKAETNMLLMSPLKEGKPHAS 360
           GVQVY+NLSHGIHGKGVSLKFG+G+SPN+ RSTPKKGLLMKAETNMLLMSPLKEGKPH +
Sbjct: 301 GVQVYRNLSHGIHGKGVSLKFGAGHSPNVWRSTPKKGLLMKAETNMLLMSPLKEGKPHTT 360

Query: 361 GLQQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWDMR 420
           GL+QLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWDMR
Sbjct: 361 GLEQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMR 420

Query: 421 DRRGMVQNI-GAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTS 480
           DRRGMVQNI G+ADNS VLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTS
Sbjct: 421 DRRGMVQNISGSADNSTVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTS 480

Query: 481 MRQAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGNK 540
           MRQAKTAFPGLGSPITHVDVTYDGKWILGTTD+YLILICTLFTDKDGNTKTGFSGRMGN+
Sbjct: 481 MRQAKTAFPGLGSPITHVDVTYDGKWILGTTDSYLILICTLFTDKDGNTKTGFSGRMGNR 540

Query: 541 IPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNS 600
           IPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNS
Sbjct: 541 IPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNS 600

Query: 601 AHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLS 649
           +HDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLS
Sbjct: 601 SHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLS 657

BLAST of Spg002699 vs. TAIR 10
Match: AT3G19240.1 (Vacuolar import/degradation, Vid27-related protein )

HSP 1 Score: 916.4 bits (2367), Expect = 1.3e-266
Identity = 474/666 (71.17%), Postives = 548/666 (82.28%), Query Frame = 0

Query: 1   MGTGHSREDVELSDSD-EYNEEEEEDDEEEEYEDAEKE-------LKPQSTKLTGTP--S 60
           MGT  SRED  +S+SD E + +  E+++E++Y+DA+K+        +P  +  +  P  S
Sbjct: 1   MGTSQSRED-RISESDTESDSDYYEEEDEDQYDDAKKQGSSSVSGTRPSDSSSSSGPTDS 60

Query: 61  AIDDVDAKLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKT 120
           +  D++ KLKALKLKY SSS S  P  KNAVKLY HIGGNTP+AKWIVS+K+T Y F+KT
Sbjct: 61  SSLDIEQKLKALKLKYPSSS-SVTPKMKNAVKLYRHIGGNTPKAKWIVSDKMTSYKFVKT 120

Query: 121 ANVDGDNNDDEE--EDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFGDQRR 180
           ++VDG++ DD +  E+ GEG         G+  W L VG KV+A VST+MQLKMFGDQRR
Sbjct: 121 SSVDGEDIDDYDDCEESGEG---------GESFWFLGVGTKVKARVSTDMQLKMFGDQRR 180

Query: 181 VDFVNKGVWALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWLKPEV 240
           VDFV+ GVWALKF +DE YR FVT FQD +FENVY ++A++EN+VK+YGK+FIGW  PE 
Sbjct: 181 VDFVSNGVWALKFLTDEDYRKFVTRFQDYLFENVYKIRASEENRVKVYGKDFIGWANPEA 240

Query: 241 ADDSIWENADIEFGKSPGPSTPSVRTKQDLIEEFEEAAN-GVQSLTLGALDNSFLVNDSG 300
           ADDS+WE+A+      P       +   DL EEFEE AN GVQSLTLGALDNSFLVND G
Sbjct: 241 ADDSMWEDAE----APPEEEETQGKRNTDLTEEFEEVANGGVQSLTLGALDNSFLVNDYG 300

Query: 301 VQVYKNLSHGIHGKGVSLKFGSGNS---PNIGRSTPKKGLLMKAETNMLLMSPLKEGKPH 360
           VQVY+N+  GIHGKGV ++F SGNS       ++TP K LLM+AETNM+LMSP K+GKP+
Sbjct: 301 VQVYRNMERGIHGKGVCVRFDSGNSKFGSGSSQTTPNKALLMRAETNMMLMSPAKQGKPN 360

Query: 361 ASGLQQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNRLCQWD 420
           ++G++QLDIE+GKIVTEWKFEKDGT+ITMRDITNDTKGSQLDPSE+TFLGLDDNRLCQWD
Sbjct: 361 STGVKQLDIESGKIVTEWKFEKDGTEITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWD 420

Query: 421 MRDRRGMVQNIGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKT 480
           MRDRRG+VQNI    +SP+L W QGHQFSRGTNFQCFATTGDGSIVVGS+DGKIRLYSKT
Sbjct: 421 MRDRRGIVQNI----DSPILEWTQGHQFSRGTNFQCFATTGDGSIVVGSLDGKIRLYSKT 480

Query: 481 SMRQAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFSGRMGN 540
           SMR AKTAFPGLGSPITHVDV+YDGKWILGTTDTYL+LICTLFTDK+G TKTGFSGRMGN
Sbjct: 481 SMRMAKTAFPGLGSPITHVDVSYDGKWILGTTDTYLVLICTLFTDKNGLTKTGFSGRMGN 540

Query: 541 KIPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKN 600
           KIPAPRLLKLTPLDSHLAG DN FHGGHFSWVTESGKQERH+VATVGKFSVIWD  RVKN
Sbjct: 541 KIPAPRLLKLTPLDSHLAGKDNKFHGGHFSWVTESGKQERHIVATVGKFSVIWDLERVKN 600

Query: 601 SAHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVS--DSPEAPLVVATPMKVSSI 649
           SAH+CYRNQQGLKSCYCYKI+LKDESIVESRFMHD F+ S   SPEAPLVVATP+KVSSI
Sbjct: 601 SAHECYRNQQGLKSCYCYKILLKDESIVESRFMHDNFSFSGNKSPEAPLVVATPLKVSSI 647

BLAST of Spg002699 vs. TAIR 10
Match: AT4G33400.1 (Vacuolar import/degradation, Vid27-related protein )

HSP 1 Score: 815.8 bits (2106), Expect = 2.4e-236
Identity = 419/669 (62.63%), Postives = 509/669 (76.08%), Query Frame = 0

Query: 1   MGTGHSREDVEL--SDSDEYNEEE---EEDDEEEEYEDAE-----------KELKPQSTK 60
           MG  HS ED+E+  SD DEY E E   E ++EEE ++D+            + L+P+S  
Sbjct: 1   MGASHSHEDLEICSSDEDEYEEYEEGREGEEEEETFQDSRDDTLGISSSGGRRLRPKSPS 60

Query: 61  LTGTPSAIDDVDAKLKALKLKYGSSSPSQIPNSKNAVKLYLHIGGNTPRAKWIVSEKLTF 120
                S++DDV+AKL+ALKLKY  +  +  P+++N+ +L+ +I GNTP+AKW+ +EKLT 
Sbjct: 61  -----SSLDDVEAKLQALKLKYPLTQSA--PSTQNSARLFRYINGNTPKAKWVTAEKLTA 120

Query: 121 YAFLKTANVDGDNNDDEEEDDGEGYKGNSSGGRGQLRWVLKVGAKVRALVSTEMQLKMFG 180
           Y F+KT   D D+ DD+E  D E              W+LKVG+K+R  VS EMQLK + 
Sbjct: 121 YCFVKTNKGDEDDEDDDENGDVENE-----------WWILKVGSKIREKVSDEMQLKAYK 180

Query: 181 DQRRVDFVNKGVWALKFPSDEQYRNFVTEFQDCMFENVYGLQATDENKVKIYGKEFIGWL 240
           DQRRVDFV K VWA+KF S E +  FV+ + +C+FEN +G++  + NK KIYGK+FIGW 
Sbjct: 181 DQRRVDFVAKAVWAMKFASSEDFSVFVSSYNNCLFENNHGVEFNEANKAKIYGKDFIGWA 240

Query: 241 KPEVADDSIWENADIEFGKSP-GPSTPSVRTKQDLIEEFEEA-ANGVQSLTLGALDNSFL 300
            PE ADDS+WE+AD    +SP G S    R  QDL E FEEA + G+ SL LGALDNSFL
Sbjct: 241 NPEAADDSMWEDADDILLQSPGGGSATPARDTQDLTEAFEEATSEGIHSLALGALDNSFL 300

Query: 301 VNDSGVQVYKNLSHGIHGKGVSLKFGSGNSPNIGR---STPKKGLLMKAETNMLLMSPLK 360
           V DSG+QV+KN+  GI GKGV + F     P  GR   S PKK LLM+AETNMLLMSP+ 
Sbjct: 301 VGDSGIQVFKNMRQGIQGKGVCVNF----EPGYGRTHSSAPKKALLMRAETNMLLMSPMS 360

Query: 361 EGKPHASGLQQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSEATFLGLDDNR 420
           +  PH+ G+ QLDIETGKI++EWKFEKDG DI+M DITND KG+QLDPS +TFLGLD+NR
Sbjct: 361 Q-TPHSRGIHQLDIETGKIISEWKFEKDGVDISMSDITNDGKGAQLDPSASTFLGLDNNR 420

Query: 421 LCQWDMRDRRGMVQNIGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIR 480
           LC+WDMRDR GMVQ++  A N+PVLNWAQGHQFSRGTNFQCFATTGDGSIVVGS+DGKIR
Sbjct: 421 LCRWDMRDRYGMVQDLATA-NTPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSLDGKIR 480

Query: 481 LYSKTSMRQAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICTLFTDKDGNTKTGFS 540
           LYS  +MRQAKTAFPGLG+P+THVD T+DGKWI+GTTDTYLI+ICTLFTDK G TKTGF 
Sbjct: 481 LYSSNTMRQAKTAFPGLGAPVTHVDATFDGKWIVGTTDTYLIVICTLFTDKSGKTKTGFE 540

Query: 541 GRMGNKIPAPRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDF 600
           GRMGNKI APRLLKL PLD+HLAG+DN F    FSWVTE GKQERH+VATVGKFSVIW+F
Sbjct: 541 GRMGNKIAAPRLLKLRPLDAHLAGSDNKFRNAQFSWVTEDGKQERHVVATVGKFSVIWNF 600

Query: 601 HRVKNSAHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKV 649
            +VKN +H+CY +Q+GLK CYCYKIVL++ESIV+SRFM+D FA+S SPEAPLV+ATPMKV
Sbjct: 601 QQVKNGSHECYHDQEGLKKCYCYKIVLRNESIVDSRFMNDNFAISGSPEAPLVIATPMKV 645

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895945.10.0e+0092.24protein CYPRO4 [Benincasa hispida][more]
XP_022149230.10.0e+0091.56protein CYPRO4 [Momordica charantia][more]
XP_023552532.10.0e+0091.88protein CYPRO4-like [Cucurbita pepo subsp. pepo][more]
XP_022984689.10.0e+0092.04protein CYPRO4-like [Cucurbita maxima][more]
KAG6576955.10.0e+0091.58Protein CYPRO4, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
P407814.3e-23078.74Protein CYPRO4 OS=Cynara cardunculus OX=4265 GN=CYPRO4 PE=2 SV=1[more]
Q555V72.5e-2826.39VID27-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274813 PE=3 SV=... [more]
Q1MTR33.6e-2726.98Vacuolar import and degradation protein 27 OS=Schizosaccharomyces pombe (strain ... [more]
P401578.9e-1827.35Vacuolar import and degradation protein 27 OS=Saccharomyces cerevisiae (strain A... [more]
Match NameE-valueIdentityDescription
A0A6J1D5600.0e+0091.56protein CYPRO4 OS=Momordica charantia OX=3673 GN=LOC111017701 PE=4 SV=1[more]
A0A6J1JB960.0e+0092.04protein CYPRO4-like OS=Cucurbita maxima OX=3661 GN=LOC111482893 PE=4 SV=1[more]
A0A6J1E9T20.0e+0091.58protein CYPRO4-like OS=Cucurbita moschata OX=3662 GN=LOC111430675 PE=4 SV=1[more]
A0A0A0LAA10.0e+0091.20VID27 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G207380 PE=4 ... [more]
A0A5D3DQK80.0e+0090.80Protein CYPRO4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1441G0002... [more]
Match NameE-valueIdentityDescription
AT3G19240.11.3e-26671.17Vacuolar import/degradation, Vid27-related protein [more]
AT4G33400.12.4e-23662.63Vacuolar import/degradation, Vid27-related protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 17..37
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..51
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 113..136
NoneNo IPR availablePANTHERPTHR31913:SF7DEM PROTEINcoord: 1..648
NoneNo IPR availableSUPERFAMILY101908Putative isomerase YbhEcoord: 348..546
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 344..526
e-value: 2.6E-8
score: 35.2
IPR013863Vacuolar import/degradation Vid27, C-terminalPFAMPF08553VID27coord: 272..591
e-value: 1.3E-42
score: 146.2
IPR040458Vacuolar import/degradation Vid27PANTHERPTHR31913VACUOLAR IMPORT AND DEGRADATION PROTEIN 27coord: 1..648

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002699.1Spg002699.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding