Spg002604 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002604
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionD-3-phosphoglycerate dehydrogenase
Locationscaffold6: 4710112 .. 4713379 (+)
RNA-Seq ExpressionSpg002604
SyntenySpg002604
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCTCCGCTTCACCTTCACTCCGTACGCCGTCGCTCCGGAACTCGCTGCTTTCTTCTCTCTCTTCTAAGCTTCCTGTTTCTTCGGCCTTTTCTGTTAATCTTCGTTCTCGGAGTGTTGGCGCGCCTCGGTTTGTGGTGCTGGTCTCTGCGTCTCTGAATGCGAAGCCTACGGTTCTTGTTGCGGAGAAGCTTGGCGAAGCGGGTCTTGATCTTCTCAAGGAATTTGCGAATGTTGATTGCTCGTATAATCTGAGTCCTGAGGAGCTCTGCACCAAGATCTCGCTCTGTGATGCGCTTATTGTGCGGAGTGGGACTAAGGTCAGCCGCGAAGTGTTTGAATCCTCTGGAGGGAGGTTGAAGGTCGTTGGGAGGGCTGGTGTTGGCATTGATAATGTGGATCTGGCGGCCGCTACTGAGCATGGTTGCTTGGTGGTGAATGCTCCGACTGCAAACACGGTAGCTGCCGCTGAGCATGGAATTGCGCTCTTGACTGCAATGGCCAGAAATGTTGCCCAAGCCGATGCGTCTGTTAAAGCTGGTGCGTCTTTTTTTTTTTTTTGGTTTGTTTTTTATGATATAAAGATTTTGTCGAACCTTCATGTTCTCTAGCTGCTTGTTTGCTTTGGATCTGCTCGAAACCATCAAAAGGGGAAAAGCTTCAGAAAGATCTCGACCTTTTATCTTAATATTGGACGAGCATTTCGTTTAAATCTTTTTTAAAATAAATATTTTAATGTTTAAAGCGAATTTTTCCTAAAGAAATTCCTTCCCGATGTGTTGATTCTGGGATTATGTTTTTTTTCTTAAAAGTATCAATACACTGTGAAAGGTGGAAACCTGTCGGAGATTTTTGTGTTTAGAATTCCCATGCTGTGCCTGCTTGGTCTGGACTCTACTGGACCTTACGATTAGTAATTTTTCTCTGTTACAGAAGAATTAAGTAATTTTTCTCTGCATCGGACATTCTCTTTCCCTTTCGAGCCCGTCGATTATGTGATGCTCAGCTTTATTGGGTGTGATGTTGTATACAAGAGGCTTTTGTTAAACGAATTATTTATGATTTTAAATTTTCTCCTCCACCGTAGGGAAGTGGCAACGTAACAAATATGTCGGTGTATCACTTGTGGGGAAAACACTGGCTGTAATGGGATTCGGCAAAGTTGGATCTGAAGTTGCTCGTCGTGCAAAGGGACTTGGCATGCATGTAATTGCACATGACCCATATGCCCCAGCTGATCGTGCTCGTGCGATAGGCGTAGAGCTTGTGAGCTTTGATGAAGCCATTGCAACTGCTGATTTCATCTCTTTACATATGCCTTTAACTCCAGCCACGTCAAAAATTCTTAATGATGAAAATTTTGCAAAGATGAAGAAAGGAGTTAGGATTGTGAATGTTGCTCGTGGAGGAGTAATCGATGAAGAAGCTCTTGTGAGGGCGCTGGATGCTGGAATTGTTGCTCAGGTTCTTTTCCTTCCTACTACCTAAAAACTGAAGTTTCCTAATTATTATATAAAACGCAAGGATTGTATAACGTTTCTTTTTCTGATAGGCTGCACTTGATGTTTTCACCGAGGAACCACCTCCTAAGGATAGCAAGTTGGTTCAGCACGAGAATGTCACTGTAACTCCTCATCTCGGTGCCAGCACAATGGAGGCTCAGGTGTGTGTTTTAAATCAAAGCACTGAAAGCTTGTGTTTTCTTTTTATAATTTATAATGATCTATATCTATATAAATTTGATGTGCAGGAAGGAGTTGCTGTTGAAATAGCCGAAGCTGTTGTTGGAGCCTTGAAAGGGGAGCTTGCGGCTACTGCTGTTAATGCTCCGATGGTTCCTGCAGAGGTAGAAACCTTTAGATTCGTAATCTTCTATCTCTCTCATGTTTCGTATTTTATGATCACTTAAAACGTTTACCTGAAGTACTTCTGTTTATGCTGACCTTAGATGCTATTTGTCGTTATTGTTTTCTTGATTGTCTTGCTTTCCAAGTGGCTTTATCTGGTTTTCTGATTTTTCTTTTGAATGCTCATCATTTATATGATTTAGAAACCTTTGACATTTATCCCCAATTTTTAAAGCTGAATATAACTTCCCATGATATAGCAATGCCTCCCTATAAATGGATTACTTTCTTTTAATAATTTGTTTCTTTTCCGTACCGATTATAAATGTCTGCTGAGTGATTTGTGATTTGCATCATTATTCATCATACATTTTATTTTCAGTGAGCAATATCTCGAGTTTCTGATATATGAGGATTCTAAGAAAGTATTTTTTTTCTTCCCTGCAGGTGTTATCAGAGCTGAAACCATTTGTTGAACTCGCTGAGAAATTGGGAAGACTGGCTGTTCAATTGGTAGCTGGAGGAAGTGGTGTGAAAACTGTGAAAGTAACATATGCTTCTTCCAGAGCTCCTGATGATCTTGATACCAGACTTCTTCGTGCCATGATAACCAAGGGTCTAATTGAGCCCATTTCCAGTGTTTTCGTGAACTTGGTGAATGCTGACTTTACTGCCAAACAGAGAGGACTAAGAATAACTGAGGAGCGGGTCGTTCTGGATGGGTCACCTGAGAAGCCATTGGAATACATTCAGGTCCAGATTGCCAACGTGGAATCCAGATTTGCCAGCGCTATATCAGACTCCGGGGAGATAAAGGTGGAAGGGCTTGTGAAAGATGGAATCCCTCACCTCACAAAGGTCGGATCTTTCGAAGTCGATGTTAGTCTGGAGGGTAGCATCATACTCTGCAGGCAGGTTGACCAGCCAGGTATGATTGGAAAAGTTGGAAGCATTCTTGGAGAAGAAAATGTTAATGTCAGCTTCATGAGTGTTGGAAGGATTGCTCCTCGAAAGCAGGCTGTTATGGCCATTGGTGTTGATGAACCGCCCAGCAAGGAATCTTTGAAGAGGATTGGTGATGTTCCAGCAATTGAAGAGTTTGTTTACCTCAAGTTGTAGAATGTGGGCATGAGCTTGACCTTCCCCTTCTCTCTCTCTCTCTCTCTCTCCCCAACCCCAAAAAAGAAAAGAGAAAAAACTCTTGTCCTTGGTCTGTGTACCACCCTCTCGGGATTTGGGTACATTCCTACATGTTTGGCCCCAATTTTGACGGTTTTTATTTAAATAAGCTGGTTTGTTTTCTTTTCGAATTGAGAAATGTGCTTTACTAAGTTGTTTGAAGAATGATTCTATACTTTGATTGGATTCTCTTGTAGTTTCTGCAGCTTGTGGAACAATTAGAAAGAGTTTAAAATGA

mRNA sequence

ATGGCTGCCTCCGCTTCACCTTCACTCCGTACGCCGTCGCTCCGGAACTCGCTGCTTTCTTCTCTCTCTTCTAAGCTTCCTGTTTCTTCGGCCTTTTCTGTTAATCTTCGTTCTCGGAGTGTTGGCGCGCCTCGGTTTGTGGTGCTGGTCTCTGCGTCTCTGAATGCGAAGCCTACGGTTCTTGTTGCGGAGAAGCTTGGCGAAGCGGGTCTTGATCTTCTCAAGGAATTTGCGAATGTTGATTGCTCGTATAATCTGAGTCCTGAGGAGCTCTGCACCAAGATCTCGCTCTGTGATGCGCTTATTGTGCGGAGTGGGACTAAGGTCAGCCGCGAAGTGTTTGAATCCTCTGGAGGGAGGTTGAAGGTCGTTGGGAGGGCTGGTGTTGGCATTGATAATGTGGATCTGGCGGCCGCTACTGAGCATGGTTGCTTGGTGGTGAATGCTCCGACTGCAAACACGGTAGCTGCCGCTGAGCATGGAATTGCGCTCTTGACTGCAATGGCCAGAAATGTTGCCCAAGCCGATGCGTCTGTTAAAGCTGGGAAGTGGCAACGTAACAAATATGTCGGTGTATCACTTGTGGGGAAAACACTGGCTGTAATGGGATTCGGCAAAGTTGGATCTGAAGTTGCTCGTCGTGCAAAGGGACTTGGCATGCATGTAATTGCACATGACCCATATGCCCCAGCTGATCGTGCTCGTGCGATAGGCGTAGAGCTTGTGAGCTTTGATGAAGCCATTGCAACTGCTGATTTCATCTCTTTACATATGCCTTTAACTCCAGCCACGTCAAAAATTCTTAATGATGAAAATTTTGCAAAGATGAAGAAAGGAGTTAGGATTGTGAATGTTGCTCGTGGAGGAGTAATCGATGAAGAAGCTCTTGTGAGGGCGCTGGATGCTGGAATTGTTGCTCAGGCTGCACTTGATGTTTTCACCGAGGAACCACCTCCTAAGGATAGCAAGTTGGTTCAGCACGAGAATGTCACTGTAACTCCTCATCTCGGTGCCAGCACAATGGAGGCTCAGGAAGGAGTTGCTGTTGAAATAGCCGAAGCTGTTGTTGGAGCCTTGAAAGGGGAGCTTGCGGCTACTGCTGTTAATGCTCCGATGGTTCCTGCAGAGGTGTTATCAGAGCTGAAACCATTTGTTGAACTCGCTGAGAAATTGGGAAGACTGGCTGTTCAATTGGTAGCTGGAGGAAGTGGTGTGAAAACTGTGAAAGTAACATATGCTTCTTCCAGAGCTCCTGATGATCTTGATACCAGACTTCTTCGTGCCATGATAACCAAGGGTCTAATTGAGCCCATTTCCAGTGTTTTCGTGAACTTGGTGAATGCTGACTTTACTGCCAAACAGAGAGGACTAAGAATAACTGAGGAGCGGGTCGTTCTGGATGGGTCACCTGAGAAGCCATTGGAATACATTCAGGTCCAGATTGCCAACGTGGAATCCAGATTTGCCAGCGCTATATCAGACTCCGGGGAGATAAAGGTGGAAGGGCTTGTGAAAGATGGAATCCCTCACCTCACAAAGGTCGGATCTTTCGAAGTCGATGTTAGTCTGGAGGGTAGCATCATACTCTGCAGGCAGGTTGACCAGCCAGGTATGATTGGAAAAGTTGGAAGCATTCTTGGAGAAGAAAATGTTAATGTCAGCTTCATGAGTGTTGGAAGGATTGCTCCTCGAAAGCAGGCTGTTATGGCCATTGGTGTTGATGAACCGCCCAGCAAGGAATCTTTGAAGAGGATTGTTTCTGCAGCTTGTGGAACAATTAGAAAGAGTTTAAAATGA

Coding sequence (CDS)

ATGGCTGCCTCCGCTTCACCTTCACTCCGTACGCCGTCGCTCCGGAACTCGCTGCTTTCTTCTCTCTCTTCTAAGCTTCCTGTTTCTTCGGCCTTTTCTGTTAATCTTCGTTCTCGGAGTGTTGGCGCGCCTCGGTTTGTGGTGCTGGTCTCTGCGTCTCTGAATGCGAAGCCTACGGTTCTTGTTGCGGAGAAGCTTGGCGAAGCGGGTCTTGATCTTCTCAAGGAATTTGCGAATGTTGATTGCTCGTATAATCTGAGTCCTGAGGAGCTCTGCACCAAGATCTCGCTCTGTGATGCGCTTATTGTGCGGAGTGGGACTAAGGTCAGCCGCGAAGTGTTTGAATCCTCTGGAGGGAGGTTGAAGGTCGTTGGGAGGGCTGGTGTTGGCATTGATAATGTGGATCTGGCGGCCGCTACTGAGCATGGTTGCTTGGTGGTGAATGCTCCGACTGCAAACACGGTAGCTGCCGCTGAGCATGGAATTGCGCTCTTGACTGCAATGGCCAGAAATGTTGCCCAAGCCGATGCGTCTGTTAAAGCTGGGAAGTGGCAACGTAACAAATATGTCGGTGTATCACTTGTGGGGAAAACACTGGCTGTAATGGGATTCGGCAAAGTTGGATCTGAAGTTGCTCGTCGTGCAAAGGGACTTGGCATGCATGTAATTGCACATGACCCATATGCCCCAGCTGATCGTGCTCGTGCGATAGGCGTAGAGCTTGTGAGCTTTGATGAAGCCATTGCAACTGCTGATTTCATCTCTTTACATATGCCTTTAACTCCAGCCACGTCAAAAATTCTTAATGATGAAAATTTTGCAAAGATGAAGAAAGGAGTTAGGATTGTGAATGTTGCTCGTGGAGGAGTAATCGATGAAGAAGCTCTTGTGAGGGCGCTGGATGCTGGAATTGTTGCTCAGGCTGCACTTGATGTTTTCACCGAGGAACCACCTCCTAAGGATAGCAAGTTGGTTCAGCACGAGAATGTCACTGTAACTCCTCATCTCGGTGCCAGCACAATGGAGGCTCAGGAAGGAGTTGCTGTTGAAATAGCCGAAGCTGTTGTTGGAGCCTTGAAAGGGGAGCTTGCGGCTACTGCTGTTAATGCTCCGATGGTTCCTGCAGAGGTGTTATCAGAGCTGAAACCATTTGTTGAACTCGCTGAGAAATTGGGAAGACTGGCTGTTCAATTGGTAGCTGGAGGAAGTGGTGTGAAAACTGTGAAAGTAACATATGCTTCTTCCAGAGCTCCTGATGATCTTGATACCAGACTTCTTCGTGCCATGATAACCAAGGGTCTAATTGAGCCCATTTCCAGTGTTTTCGTGAACTTGGTGAATGCTGACTTTACTGCCAAACAGAGAGGACTAAGAATAACTGAGGAGCGGGTCGTTCTGGATGGGTCACCTGAGAAGCCATTGGAATACATTCAGGTCCAGATTGCCAACGTGGAATCCAGATTTGCCAGCGCTATATCAGACTCCGGGGAGATAAAGGTGGAAGGGCTTGTGAAAGATGGAATCCCTCACCTCACAAAGGTCGGATCTTTCGAAGTCGATGTTAGTCTGGAGGGTAGCATCATACTCTGCAGGCAGGTTGACCAGCCAGGTATGATTGGAAAAGTTGGAAGCATTCTTGGAGAAGAAAATGTTAATGTCAGCTTCATGAGTGTTGGAAGGATTGCTCCTCGAAAGCAGGCTGTTATGGCCATTGGTGTTGATGAACCGCCCAGCAAGGAATCTTTGAAGAGGATTGTTTCTGCAGCTTGTGGAACAATTAGAAAGAGTTTAAAATGA

Protein sequence

MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTVLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQIANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIGKVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRIVSAACGTIRKSLK
Homology
BLAST of Spg002604 vs. NCBI nr
Match: XP_008456163.1 (PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis melo] >KAA0037176.1 D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa] >TYK13897.1 D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.8e-307
Identity = 569/585 (97.26%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASP+LRTPSLR SLLSS SSKLP+ SAFSVNLR RSVGAPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRTSLLSSFSSKLPLPSAFSVNLRPRSVGAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV FDEAIATADF+SLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFDEAIATADFLSLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISDSGEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSKESLKRI
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRI 585

BLAST of Spg002604 vs. NCBI nr
Match: XP_038880544.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1063.9 bits (2750), Expect = 5.0e-307
Identity = 570/585 (97.44%), Postives = 580/585 (99.15%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASP+L  PSLRNS+LSSLSSKLPV SAFSVNLR RSVGAPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLPIPSLRNSVLSSLSSKLPVPSAFSVNLRPRSVGAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAIATADFISLHMPLTPATSKILND+NFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIATADFISLHMPLTPATSKILNDDNFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTEEPPPK+SKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTEEPPPKESKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISD+GEI+VEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDTGEIRVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSKESLKRI
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRI 585

BLAST of Spg002604 vs. NCBI nr
Match: XP_004140713.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis sativus] >KGN57501.1 hypothetical protein Csa_011422 [Cucumis sativus])

HSP 1 Score: 1063.5 bits (2749), Expect = 6.5e-307
Identity = 569/585 (97.26%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASP+LRTPSLRNSL SSLSSKLP+ SAFSVNLR RSV APRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRNSLFSSLSSKLPLPSAFSVNLRPRSVAAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV F+EAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFEEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISDSGEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSKESLKRI
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRI 585

BLAST of Spg002604 vs. NCBI nr
Match: XP_022942525.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.3e-304
Identity = 563/585 (96.24%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASPSLRT ++RNS+LSSLSSKLP+SSAFSVN R RSVG PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSTVRNSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAI TADFISLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISD+GEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSK++LKRI
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRI 585

BLAST of Spg002604 vs. NCBI nr
Match: XP_022979807.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1053.9 bits (2724), Expect = 5.2e-304
Identity = 563/585 (96.24%), Postives = 577/585 (98.63%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASPSLRT  LR+S+LSSLSSKLP+SSAFSVN R RSVG PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSKLRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAI TADFISLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISD+GEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSK++LKRI
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRI 585

BLAST of Spg002604 vs. ExPASy Swiss-Prot
Match: O49485 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH1 PE=1 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 8.2e-260
Identity = 484/587 (82.45%), Postives = 534/587 (90.97%), Query Frame = 0

Query: 2   AASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGA-PRFVVLVSASL--NAKP 61
           AAS+S ++ T SLRN    +LSS+ P+ SA SV   SR      R +VLVS S    +KP
Sbjct: 6   AASSSIAVATNSLRN---VTLSSRSPLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKP 65

Query: 62  TVLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSG 121
           T+LVAEKLG+AG+ LL++ ANVDCSYN++PEEL  KISLCDALIVRSGTKV REVFESS 
Sbjct: 66  TILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSH 125

Query: 122 GRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADAS 181
           GRLKVVGRAGVGIDNVDL+AATE GCLVVNAPTANT+AAAEHGIAL+ AMARNVAQADAS
Sbjct: 126 GRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADAS 185

Query: 182 VKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIG 241
           VKAG+W+RNKYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM VIAHDPYAPADRA AIG
Sbjct: 186 VKAGEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIG 245

Query: 242 VELVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVR 301
           V+LVSFDEA+ATADFISLHMPLTP TSKILNDE FAKMKKGVRIVNVARGGVIDE+ALVR
Sbjct: 246 VDLVSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVR 305

Query: 302 ALDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGA 361
           ALDAGIVAQAALDVFT+EPP KDSKLVQHE VTVTPHLGASTMEAQEGVA+EIAEAVVGA
Sbjct: 306 ALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGA 365

Query: 362 LKGELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAP 421
           L GELAATAVNAPMV AEVL+ELKP+V LAEKLGRLAVQLVAGGSGVK  K+TYAS+RA 
Sbjct: 366 LNGELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARAT 425

Query: 422 DDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQ 481
           DDLDTRLLRAMITKG+IEPIS V+VNLVNADFTAKQRGLR++EERV+LDGSPE PLE I 
Sbjct: 426 DDLDTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETIT 485

Query: 482 VQIANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGM 541
           VQ++NVES+FAS++S+SGE+KVEG VKDG+PHLTKVGSFEVDV+LEGSIILCRQVDQPGM
Sbjct: 486 VQLSNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGM 545

Query: 542 IGKVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           IG VGSILGE NVNV+FMSVGRIAPRKQA+MAIGVD+ PSKE+LK+I
Sbjct: 546 IGTVGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKI 589

BLAST of Spg002604 vs. ExPASy Swiss-Prot
Match: Q9LT69 (D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH3 PE=1 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 6.3e-252
Identity = 465/575 (80.87%), Postives = 515/575 (89.57%), Query Frame = 0

Query: 13  SLRNSLLSSLSSKLPV--SSAFSVNLRSRSVGAPRFVVLVSASLNAKPTVLVAEKLGEAG 72
           SL  S + S SS+L    SS F +  R R +     V   S+    KPT+LV EKLG+AG
Sbjct: 4   SLNLSSIFSSSSRLVTTPSSVFPIRQRRRII----LVTSSSSGGGGKPTILVTEKLGQAG 63

Query: 73  LDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGRLKVVGRAGVG 132
           +DLLK++ANVDCSY+LS EELCTKISLCDALIVRSGTKV R+VFESS GRLKVVGRAGVG
Sbjct: 64  IDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRAGVG 123

Query: 133 IDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVKAGKWQRNKYV 192
           IDNVDLAAATE+GCLVVNAPTANTVAAAEHGIALLTAMARN+AQADAS+KAGKW RNKYV
Sbjct: 124 IDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRNKYV 183

Query: 193 GVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEAIAT 252
           GVSLVGKTLAV+GFGKVGSEVARRA+GLGMHVI HDPYAPADRARAIGVELVSF+ AI+T
Sbjct: 184 GVSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVAIST 243

Query: 253 ADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQAAL 312
           ADFISLH+PLT ATSK++ND  FA MKKGVRIVNVARGGVIDEEAL+RALD+GIVAQAAL
Sbjct: 244 ADFISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQAAL 303

Query: 313 DVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATAVNA 372
           DVFT EPP KD+KLV HE+VT TPHLGASTMEAQEGV++E+AEAV+GAL+GELAATAVNA
Sbjct: 304 DVFTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATAVNA 363

Query: 373 PMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLRAMI 432
           PMVP EVL ELKP+V LAEKLGRLAVQLV GGSGV  VKVTYASSRAPDDLDTRLLRAM+
Sbjct: 364 PMVPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLRAMV 423

Query: 433 TKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQIANVESRFAS 492
            KG+IEPISSVF+NLVN+D+ AKQRG++I+EER+VLDGSPE P+EYI V+IANVESRFAS
Sbjct: 424 IKGIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESRFAS 483

Query: 493 AISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIGKVGSILGEEN 552
           A+S+SGEIKVEG VK G+P LTKVG F VDVSLEGS+ILCRQVDQPGMIGKV SILG+EN
Sbjct: 484 ALSESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILGDEN 543

Query: 553 VNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           VNVSFMSVGRIAP KQAVMAIGVDE PSKE+LK+I
Sbjct: 544 VNVSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKI 574

BLAST of Spg002604 vs. ExPASy Swiss-Prot
Match: O04130 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH2 PE=1 SV=2)

HSP 1 Score: 815.5 bits (2105), Expect = 4.1e-235
Identity = 418/529 (79.02%), Postives = 481/529 (90.93%), Query Frame = 0

Query: 57  KPTVLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFES 116
           KP +LV EKLGEAG++LL+EF +VDCSY+LSPE+L  K++  DALIVRSGTKV+REVFE+
Sbjct: 82  KPRILVTEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEA 141

Query: 117 SGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQAD 176
           + GRLKVVGRAGVGIDNVDL AATEHGCLVVNAPTANTVAAAEHGIALL +MARNVAQAD
Sbjct: 142 AKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQAD 201

Query: 177 ASVKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARA 236
           AS+KAGKW+R+KYVGVSLVGKTLAVMGFGKVG+EVARRAKGLGM VI+HDPYAPADRARA
Sbjct: 202 ASIKAGKWERSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRARA 261

Query: 237 IGVELVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEAL 296
           +GV+LVSFD+AI+TADF+SLHMPLTPAT K+ NDE F+KMKKGVR++NVARGGVIDE+AL
Sbjct: 262 LGVDLVSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDAL 321

Query: 297 VRALDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVV 356
           VRALDAGIVAQAALDVF EEPP KDS+L+QHENVTVTPHLGAST EAQEGVA+EIAEAV 
Sbjct: 322 VRALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVA 381

Query: 357 GALKGELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSR 416
           GALKGEL+ATAVNAPMV  EVLSEL P++ LAEKLGRLAVQL +GG GV++++V Y S+R
Sbjct: 382 GALKGELSATAVNAPMVAPEVLSELTPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSAR 441

Query: 417 APDDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEY 476
             DDLDTRLLRAMITKG+IEPIS  +VNLVNADF AKQ+GLRI+EER+V+D SPE P++ 
Sbjct: 442 DRDDLDTRLLRAMITKGIIEPISDSYVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDS 501

Query: 477 IQVQIANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQP 536
           IQVQI NVES FA A+SD+G+I +EG VK G+PHLT VGSF VDVSLEG++ILCRQVDQP
Sbjct: 502 IQVQILNVESNFAGAVSDAGDISIEGKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQP 561

Query: 537 GMIGKVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           GMIG+VG+ILGE+NVNV+FMSVGR   RKQA+MAIGVDE P  ++L+RI
Sbjct: 562 GMIGQVGNILGEQNVNVNFMSVGRTVLRKQAIMAIGVDEEPDNKTLERI 610

BLAST of Spg002604 vs. ExPASy Swiss-Prot
Match: Q58424 (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=serA PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.8e-105
Identity = 224/527 (42.50%), Postives = 325/527 (61.67%), Query Frame = 0

Query: 60  VLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGG 119
           +LV + L E  + +L+E   V+ +  L+ EEL  KI   D L+VRSGTKV+R+V E +  
Sbjct: 4   ILVTDPLHEDAIKILEEVGEVEVATGLTKEELLEKIKDADVLVVRSGTKVTRDVIEKA-E 63

Query: 120 RLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASV 179
           +LKV+GRAGVG+DN+D+ AATE G +VVNAP A++++ AE  + L+ A ARN+ QA AS+
Sbjct: 64  KLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASL 123

Query: 180 KAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGV 239
           K G+W R ++ G+ L GKTL V+G G++G +V +RAK  GM++I +DPY P + A ++GV
Sbjct: 124 KRGEWDRKRFKGIELYGKTLGVIGLGRIGQQVVKRAKAFGMNIIGYDPYIPKEVAESMGV 183

Query: 240 ELV-SFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVR 299
           ELV   +E    ADFI+LH+PLTP T  I+  E  A MKK   IVN ARGG+IDE+AL  
Sbjct: 184 ELVDDINELCKRADFITLHVPLTPKTRHIIGREQIALMKKNAIIVNCARGGLIDEKALYE 243

Query: 300 ALDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGA 359
           AL  G +  AALDVF EE PPKD+ L+  +NV  TPH GAST EAQ+     +AE +   
Sbjct: 244 ALKEGKIRAAALDVF-EEEPPKDNPLLTLDNVIGTPHQGASTEEAQKAAGTIVAEQIKKV 303

Query: 360 LKGELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAP 419
           L+GELA   VN P +P E L +LKP++ LAE LG + +Q++ G   V  V++ Y+   A 
Sbjct: 304 LRGELAENVVNMPNIPQEKLGKLKPYMLLAEMLGNIVMQVLDG--SVNRVELIYSGELAK 363

Query: 420 DDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQ 479
           +  D  L++    KGL+ PI    +NLVNA   AK R + + E       S EK    I+
Sbjct: 364 EKTD--LIKRAFLKGLLSPILLAGINLVNAPIIAKNRNINVVESST----SEEKYGNAIK 423

Query: 480 VQIANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGM 539
           +          +A SD  +  + G + +  P + +V  +EV    EG + + + +D+PG 
Sbjct: 424 I----------TAESDKKKFSIVGAIINNKPVILEVDGYEVSFIPEGVLAIIKHIDRPGT 483

Query: 540 IGKVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           IG+V   LG+  +N++ M VGR  P  ++VM + +D    +E +++I
Sbjct: 484 IGRVCITLGDYGINIASMQVGRKEPGGESVMLLNLDHTVPEEVIEKI 510

BLAST of Spg002604 vs. ExPASy Swiss-Prot
Match: O27051 (D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=serA PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.3e-102
Identity = 213/528 (40.34%), Postives = 323/528 (61.17%), Query Frame = 0

Query: 60  VLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGG 119
           VL+A+ + E G+  L+E A V  +  ++PEEL   I   DA++VRS TKV+REV E++  
Sbjct: 6   VLIADSINEKGISELEEVAEVVVNTTITPEELLDAIKDFDAIVVRSRTKVTREVIEAA-P 65

Query: 120 RLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASV 179
           RLK++ RAGVG+DNVD+ AAT+ G +V+NAP + ++  AEH I L+ A+AR +A AD SV
Sbjct: 66  RLKIIARAGVGVDNVDVKAATDRGIMVINAPESTSITVAEHSIGLMLALARKIAIADRSV 125

Query: 180 KAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGV 239
           K GKW++N+++G+ L GKTL ++G G++GS+V  R K  GM ++ +DPY   + A  +GV
Sbjct: 126 KEGKWEKNRFMGIELNGKTLGIIGMGRIGSQVVVRTKAFGMDIMVYDPYISKEAAEEMGV 185

Query: 240 ELVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRA 299
            +   +  +  +D +++H+PLTP T  +++++ F  MK    IVN ARGG+IDE+AL RA
Sbjct: 186 TVTDLETLLRESDIVTIHVPLTPETRHLISEDEFKLMKDTAFIVNCARGGIIDEDALYRA 245

Query: 300 LDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGAL 359
           L  G +A AALDVF EE PP+ S L++ ENV +TPH+GAST EAQ   A+ +A  +    
Sbjct: 246 LKDGEIAGAALDVF-EEEPPEGSPLLELENVVLTPHIGASTSEAQRDAAIIVANEIKTVF 305

Query: 360 KGELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPD 419
           +G      +N P++ +E    LKP++ELAEK+G +  Q + G   ++ + VTY    A  
Sbjct: 306 QGGAPRNVLNMPVMDSETYKSLKPYIELAEKMGAIIAQALPG--NIEKLDVTYCGELAEM 365

Query: 420 DLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITE-ERVVLDGSPEKPLEYIQ 479
             D  +L   + + ++ PI +  VNL+NA   AK+RG+ +TE  R   DG       Y  
Sbjct: 366 QFD--ILTRTMLQAILNPILTEPVNLINAPSIAKKRGIMVTEARRSESDG-------YRS 425

Query: 480 VQIANVESRFASAISDSGEIKVEGL-VKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPG 539
           + IA  E       SD G   VE   +K+  P +  +  + VDV  EG++I+ R  D PG
Sbjct: 426 IIIATAE-------SDRGGFSVEATHIKE--PTIIGINGYRVDVKPEGTMIIARYRDLPG 485

Query: 540 MIGKVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
            IG +G+ LG+  +N++ M VGR     +AVM + VD+    E ++ +
Sbjct: 486 TIGAIGTKLGQHGINIATMQVGRKEIGGEAVMVLKVDQSVPAEVIEEV 511

BLAST of Spg002604 vs. ExPASy TrEMBL
Match: A0A5D3CPP5 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G00870 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.9e-307
Identity = 569/585 (97.26%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASP+LRTPSLR SLLSS SSKLP+ SAFSVNLR RSVGAPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRTSLLSSFSSKLPLPSAFSVNLRPRSVGAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV FDEAIATADF+SLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFDEAIATADFLSLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISDSGEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSKESLKRI
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRI 585

BLAST of Spg002604 vs. ExPASy TrEMBL
Match: A0A1S3C2L0 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496183 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.9e-307
Identity = 569/585 (97.26%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASP+LRTPSLR SLLSS SSKLP+ SAFSVNLR RSVGAPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRTSLLSSFSSKLPLPSAFSVNLRPRSVGAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV FDEAIATADF+SLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFDEAIATADFLSLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISDSGEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSKESLKRI
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRI 585

BLAST of Spg002604 vs. ExPASy TrEMBL
Match: A0A0A0LBW6 (D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G199630 PE=3 SV=1)

HSP 1 Score: 1063.5 bits (2749), Expect = 3.2e-307
Identity = 569/585 (97.26%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASP+LRTPSLRNSL SSLSSKLP+ SAFSVNLR RSV APRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRNSLFSSLSSKLPLPSAFSVNLRPRSVAAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV F+EAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFEEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISDSGEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSKESLKRI
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRI 585

BLAST of Spg002604 vs. ExPASy TrEMBL
Match: A0A6J1FWG6 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111447534 PE=3 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.1e-304
Identity = 563/585 (96.24%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASPSLRT ++RNS+LSSLSSKLP+SSAFSVN R RSVG PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSTVRNSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAI TADFISLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISD+GEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSK++LKRI
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRI 585

BLAST of Spg002604 vs. ExPASy TrEMBL
Match: A0A6J1IPQ1 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479396 PE=3 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.5e-304
Identity = 563/585 (96.24%), Postives = 577/585 (98.63%), Query Frame = 0

Query: 1   MAASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGAPRFVVLVSASLNAKPTV 60
           MAASASPSLRT  LR+S+LSSLSSKLP+SSAFSVN R RSVG PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSKLRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAI TADFISLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFT EPPPKDSKL+QHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVL+ELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISD+GEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDE PSK++LKRI
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRI 585

BLAST of Spg002604 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 897.5 bits (2318), Expect = 5.8e-261
Identity = 484/587 (82.45%), Postives = 534/587 (90.97%), Query Frame = 0

Query: 2   AASASPSLRTPSLRNSLLSSLSSKLPVSSAFSVNLRSRSVGA-PRFVVLVSASL--NAKP 61
           AAS+S ++ T SLRN    +LSS+ P+ SA SV   SR      R +VLVS S    +KP
Sbjct: 6   AASSSIAVATNSLRN---VTLSSRSPLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKP 65

Query: 62  TVLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSG 121
           T+LVAEKLG+AG+ LL++ ANVDCSYN++PEEL  KISLCDALIVRSGTKV REVFESS 
Sbjct: 66  TILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSH 125

Query: 122 GRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADAS 181
           GRLKVVGRAGVGIDNVDL+AATE GCLVVNAPTANT+AAAEHGIAL+ AMARNVAQADAS
Sbjct: 126 GRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADAS 185

Query: 182 VKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIG 241
           VKAG+W+RNKYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM VIAHDPYAPADRA AIG
Sbjct: 186 VKAGEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIG 245

Query: 242 VELVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVR 301
           V+LVSFDEA+ATADFISLHMPLTP TSKILNDE FAKMKKGVRIVNVARGGVIDE+ALVR
Sbjct: 246 VDLVSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVR 305

Query: 302 ALDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGA 361
           ALDAGIVAQAALDVFT+EPP KDSKLVQHE VTVTPHLGASTMEAQEGVA+EIAEAVVGA
Sbjct: 306 ALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGA 365

Query: 362 LKGELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAP 421
           L GELAATAVNAPMV AEVL+ELKP+V LAEKLGRLAVQLVAGGSGVK  K+TYAS+RA 
Sbjct: 366 LNGELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARAT 425

Query: 422 DDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQ 481
           DDLDTRLLRAMITKG+IEPIS V+VNLVNADFTAKQRGLR++EERV+LDGSPE PLE I 
Sbjct: 426 DDLDTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETIT 485

Query: 482 VQIANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGM 541
           VQ++NVES+FAS++S+SGE+KVEG VKDG+PHLTKVGSFEVDV+LEGSIILCRQVDQPGM
Sbjct: 486 VQLSNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGM 545

Query: 542 IGKVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           IG VGSILGE NVNV+FMSVGRIAPRKQA+MAIGVD+ PSKE+LK+I
Sbjct: 546 IGTVGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKI 589

BLAST of Spg002604 vs. TAIR 10
Match: AT3G19480.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 871.3 bits (2250), Expect = 4.4e-253
Identity = 465/575 (80.87%), Postives = 515/575 (89.57%), Query Frame = 0

Query: 13  SLRNSLLSSLSSKLPV--SSAFSVNLRSRSVGAPRFVVLVSASLNAKPTVLVAEKLGEAG 72
           SL  S + S SS+L    SS F +  R R +     V   S+    KPT+LV EKLG+AG
Sbjct: 4   SLNLSSIFSSSSRLVTTPSSVFPIRQRRRII----LVTSSSSGGGGKPTILVTEKLGQAG 63

Query: 73  LDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGRLKVVGRAGVG 132
           +DLLK++ANVDCSY+LS EELCTKISLCDALIVRSGTKV R+VFESS GRLKVVGRAGVG
Sbjct: 64  IDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRAGVG 123

Query: 133 IDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVKAGKWQRNKYV 192
           IDNVDLAAATE+GCLVVNAPTANTVAAAEHGIALLTAMARN+AQADAS+KAGKW RNKYV
Sbjct: 124 IDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRNKYV 183

Query: 193 GVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEAIAT 252
           GVSLVGKTLAV+GFGKVGSEVARRA+GLGMHVI HDPYAPADRARAIGVELVSF+ AI+T
Sbjct: 184 GVSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVAIST 243

Query: 253 ADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQAAL 312
           ADFISLH+PLT ATSK++ND  FA MKKGVRIVNVARGGVIDEEAL+RALD+GIVAQAAL
Sbjct: 244 ADFISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQAAL 303

Query: 313 DVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATAVNA 372
           DVFT EPP KD+KLV HE+VT TPHLGASTMEAQEGV++E+AEAV+GAL+GELAATAVNA
Sbjct: 304 DVFTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATAVNA 363

Query: 373 PMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLRAMI 432
           PMVP EVL ELKP+V LAEKLGRLAVQLV GGSGV  VKVTYASSRAPDDLDTRLLRAM+
Sbjct: 364 PMVPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLRAMV 423

Query: 433 TKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEYIQVQIANVESRFAS 492
            KG+IEPISSVF+NLVN+D+ AKQRG++I+EER+VLDGSPE P+EYI V+IANVESRFAS
Sbjct: 424 IKGIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESRFAS 483

Query: 493 AISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIGKVGSILGEEN 552
           A+S+SGEIKVEG VK G+P LTKVG F VDVSLEGS+ILCRQVDQPGMIGKV SILG+EN
Sbjct: 484 ALSESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILGDEN 543

Query: 553 VNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           VNVSFMSVGRIAP KQAVMAIGVDE PSKE+LK+I
Sbjct: 544 VNVSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKI 574

BLAST of Spg002604 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 815.5 bits (2105), Expect = 2.9e-236
Identity = 418/529 (79.02%), Postives = 481/529 (90.93%), Query Frame = 0

Query: 57  KPTVLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFES 116
           KP +LV EKLGEAG++LL+EF +VDCSY+LSPE+L  K++  DALIVRSGTKV+REVFE+
Sbjct: 82  KPRILVTEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEA 141

Query: 117 SGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQAD 176
           + GRLKVVGRAGVGIDNVDL AATEHGCLVVNAPTANTVAAAEHGIALL +MARNVAQAD
Sbjct: 142 AKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQAD 201

Query: 177 ASVKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARA 236
           AS+KAGKW+R+KYVGVSLVGKTLAVMGFGKVG+EVARRAKGLGM VI+HDPYAPADRARA
Sbjct: 202 ASIKAGKWERSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRARA 261

Query: 237 IGVELVSFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEAL 296
           +GV+LVSFD+AI+TADF+SLHMPLTPAT K+ NDE F+KMKKGVR++NVARGGVIDE+AL
Sbjct: 262 LGVDLVSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDAL 321

Query: 297 VRALDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVV 356
           VRALDAGIVAQAALDVF EEPP KDS+L+QHENVTVTPHLGAST EAQEGVA+EIAEAV 
Sbjct: 322 VRALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVA 381

Query: 357 GALKGELAATAVNAPMVPAEVLSELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSR 416
           GALKGEL+ATAVNAPMV  EVLSEL P++ LAEKLGRLAVQL +GG GV++++V Y S+R
Sbjct: 382 GALKGELSATAVNAPMVAPEVLSELTPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSAR 441

Query: 417 APDDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVVLDGSPEKPLEY 476
             DDLDTRLLRAMITKG+IEPIS  +VNLVNADF AKQ+GLRI+EER+V+D SPE P++ 
Sbjct: 442 DRDDLDTRLLRAMITKGIIEPISDSYVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDS 501

Query: 477 IQVQIANVESRFASAISDSGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQP 536
           IQVQI NVES FA A+SD+G+I +EG VK G+PHLT VGSF VDVSLEG++ILCRQVDQP
Sbjct: 502 IQVQILNVESNFAGAVSDAGDISIEGKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQP 561

Query: 537 GMIGKVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEPPSKESLKRI 586
           GMIG+VG+ILGE+NVNV+FMSVGR   RKQA+MAIGVDE P  ++L+RI
Sbjct: 562 GMIGQVGNILGEQNVNVNFMSVGRTVLRKQAIMAIGVDEEPDNKTLERI 610

BLAST of Spg002604 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 795.0 bits (2052), Expect = 4.0e-230
Identity = 417/556 (75.00%), Postives = 481/556 (86.51%), Query Frame = 0

Query: 57  KPTVLVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFES 116
           KP +LV EKLGEAG++LL+EF +VDCSY+LSPE+L  K++  DALIVRSGTKV+REVFE+
Sbjct: 82  KPRILVTEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEA 141

Query: 117 SGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQAD 176
           + GRLKVVGRAGVGIDNVDL AATEHGCLVVNAPTANTVAAAEHGIALL +MARNVAQAD
Sbjct: 142 AKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQAD 201

Query: 177 ASVKAG-----------KW----------------QRNKYVGVSLVGKTLAVMGFGKVGS 236
           AS+KAG           +W                +R+KYVGVSLVGKTLAVMGFGKVG+
Sbjct: 202 ASIKAGTLNYLFLVLLLRWNCRQSKHQYTIETETEKRSKYVGVSLVGKTLAVMGFGKVGT 261

Query: 237 EVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEAIATADFISLHMPLTPATSKILN 296
           EVARRAKGLGM VI+HDPYAPADRARA+GV+LVSFD+AI+TADF+SLHMPLTPAT K+ N
Sbjct: 262 EVARRAKGLGMTVISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLTPATKKVFN 321

Query: 297 DENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQAALDVFTEEPPPKDSKLVQHEN 356
           DE F+KMKKGVR++NVARGGVIDE+ALVRALDAGIVAQAALDVF EEPP KDS+L+QHEN
Sbjct: 322 DETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRLIQHEN 381

Query: 357 VTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATAVNAPMVPAEVLSELKPFVELAE 416
           VTVTPHLGAST EAQEGVA+EIAEAV GALKGEL+ATAVNAPMV  EVLSEL P++ LAE
Sbjct: 382 VTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMVAPEVLSELTPYIVLAE 441

Query: 417 KLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLRAMITKGLIEPISSVFVNLVNAD 476
           KLGRLAVQL +GG GV++++V Y S+R  DDLDTRLLRAMITKG+IEPIS  +VNLVNAD
Sbjct: 442 KLGRLAVQLASGGKGVQSIRVVYRSARDRDDLDTRLLRAMITKGIIEPISDSYVNLVNAD 501

Query: 477 FTAKQRGLRITEERVVLDGSPEKPLEYIQVQIANVESRFASAISDSGEIKVEGLVKDGIP 536
           F AKQ+GLRI+EER+V+D SPE P++ IQVQI NVES FA A+SD+G+I +EG VK G+P
Sbjct: 502 FIAKQKGLRISEERMVVDSSPEYPVDSIQVQILNVESNFAGAVSDAGDISIEGKVKYGVP 561

Query: 537 HLTKVGSFEVDVSLEGSIILCRQVDQPGMIGKVGSILGEENVNVSFMSVGRIAPRKQAVM 586
           HLT VGSF VDVSLEG++ILCRQVDQPGMIG+VG+ILGE+NVNV+FMSVGR   RKQA+M
Sbjct: 562 HLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVNFMSVGRTVLRKQAIM 621

BLAST of Spg002604 vs. TAIR 10
Match: AT5G14780.1 (formate dehydrogenase )

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-25
Identity = 79/246 (32.11%), Postives = 126/246 (51.22%), Query Frame = 0

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LK++  AG+G D++DL AA   G  V     +N V+ AE  +  +  + RN       V 
Sbjct: 120 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 179

Query: 181 AGKWQ--RNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPA-DRARAI 240
            G+W      Y    L GKT+  +G G++G  + +R K  G +++ HD    A +  +  
Sbjct: 180 KGEWNVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAPELEKET 239

Query: 241 GVELV-SFDEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEAL 300
           G + V   +E +   D I ++MPLT  T  + N E   K+KKGV IVN ARG +++ +A+
Sbjct: 240 GAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQAV 299

Query: 301 VRALDAGIVAQAALDVFTEEPPPKDSKLVQHENVTVTPHLGASTMEAQEGVAVEIAEAVV 360
           V A+++G +   + DV+  +P PKD       N  +TPH   +T++AQ   A    + + 
Sbjct: 300 VDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAMTPHTSGTTIDAQLRYAAGTKDMLE 359

Query: 361 GALKGE 363
              KGE
Sbjct: 360 RYFKGE 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008456163.13.8e-30797.26PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis melo] >K... [more]
XP_038880544.15.0e-30797.44D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida][more]
XP_004140713.16.5e-30797.26D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis sativus] >KGN57501.... [more]
XP_022942525.12.3e-30496.24D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita moschata][more]
XP_022979807.15.2e-30496.24D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O494858.2e-26082.45D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9LT696.3e-25280.87D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
O041304.1e-23579.02D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q584241.8e-10542.50D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC... [more]
O270515.3e-10240.34D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (st... [more]
Match NameE-valueIdentityDescription
A0A5D3CPP51.9e-30797.26D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C2L01.9e-30797.26D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496183 PE=3 ... [more]
A0A0A0LBW63.2e-30797.26D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G199630 PE... [more]
A0A6J1FWG61.1e-30496.24D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111447534... [more]
A0A6J1IPQ12.5e-30496.24D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479396 P... [more]
Match NameE-valueIdentityDescription
AT4G34200.15.8e-26182.45D-3-phosphoglycerate dehydrogenase [more]
AT3G19480.14.4e-25380.87D-3-phosphoglycerate dehydrogenase [more]
AT1G17745.12.9e-23679.02D-3-phosphoglycerate dehydrogenase [more]
AT1G17745.24.0e-23075.00D-3-phosphoglycerate dehydrogenase [more]
AT5G14780.11.9e-2532.11formate dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015878S-adenosyl-L-homocysteine hydrolase, NAD binding domainSMARTSM00997AdoHcyase_NAD_2coord: 187..356
e-value: 8.1E-4
score: -30.2
NoneNo IPR availableGENE3D3.40.50.720coord: 62..362
e-value: 3.9E-111
score: 372.5
NoneNo IPR availableGENE3D3.30.70.260coord: 526..593
e-value: 9.8E-19
score: 69.1
NoneNo IPR availableGENE3D3.40.50.720coord: 153..335
e-value: 3.9E-111
score: 372.5
NoneNo IPR availablePANTHERPTHR42938FORMATE DEHYDROGENASE 1coord: 35..588
NoneNo IPR availablePANTHERPTHR42938:SF35D-3-PHOSPHOGLYCERATE DEHYDROGENASEcoord: 35..588
NoneNo IPR availableCDDcd04902ACT_3PGDH-xctcoord: 528..585
e-value: 6.59426E-20
score: 82.1325
NoneNo IPR availableCDDcd12173PGDH_4coord: 60..362
e-value: 2.67223E-164
score: 468.819
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 517..585
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 57..190
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 60..369
e-value: 8.6E-35
score: 120.3
IPR002912ACT domainPFAMPF01842ACTcoord: 527..585
e-value: 3.1E-7
score: 30.1
IPR002912ACT domainPROSITEPS51671ACTcoord: 527..598
score: 9.896772
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 162..337
e-value: 6.6E-64
score: 214.5
IPR029009Allosteric substrate binding domain superfamilyGENE3D3.30.1330.90coord: 373..525
e-value: 9.3E-31
score: 108.5
IPR029009Allosteric substrate binding domain superfamilySUPERFAMILY143548Serine metabolism enzymes domaincoord: 375..521
IPR006236D-3-phosphoglycerate dehydrogenaseTIGRFAMTIGR01327TIGR01327coord: 60..586
e-value: 2.2E-168
score: 559.3
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00670D_2_HYDROXYACID_DH_2coord: 247..269
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00671D_2_HYDROXYACID_DH_3coord: 276..292
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 199..226
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 156..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002604.1Spg002604.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006564 L-serine biosynthetic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0051287 NAD binding
molecular_function GO:0004617 phosphoglycerate dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor