Spg002568 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002568
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	dihydrofolate synthetase isoform X1
Location	scaffold6: 898289 .. 903592 (-)
RNA-Seq Expression	Spg002568
Synteny	Spg002568

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTGAATTGCCTAAAGAAATTTTGAACTTTTTAATGGGCATCTGGAATTGAAAGGAAATGATTTTAAATTCCAAGATTTGAAAAAATGGAAATGATTTTAAAATCCTAATCTTGTTTGGGAACCTCGGAGATTTTGCTGGATTTTAAATCAGTAAAAATAACATTTATTGTTTGGATGTTCTAAAGAAATGAAAAGAAAATAGCAATTTTACAATTACACTGTATTATGTGATTTTCAATTTTCTCGTCGAGGATTGTTGCAAGTCCAAGAGTTCGAGCTGTAGCCACTCTTCGAGCTCGTAATCGCATTTCCTTCAAGTTCTTCTTTTTCCCTCAAGAAACAATCATGATTTCAGGATGAATATCTTCAAACATCATCACCACTGTCGCCCACAGATTCTCGGAAGGCTTCTCCTAAATTATTTCGCTGGTGAAAGGCCTTCTATCAATCGAATAATAGGGTCCAAACAATGCTTCGGCACTCACTCAGAAGATCAAGAGATGACGGATTTCATGGAATATTTAGATTCCCTGAAGAATTATGAGAAATTGGGTGTGCCCAGAGGCGCAGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGCTGATGGAGCGGCTGGGCAATCCACAATCGAAGTTTAAGGTTGGCTCATTATGATGATGAAGATTAAATTTTTAGTTTTCCACTTTTAGTGTTAAGTAAATCTTGCAGTTTTGGACTGACCCATTTCCTCTGCAATTCAACTGAGTTTAATTAGACTTGAAAGCGTATGGTAAATCAGGCTAGGCTACCGTAAGAGTTAGTCTTTTATTCATGTACTTAACATTCTTCCATTGGGTGTAAATTAACTTTTTTCTAGGAGCGGAGATTCGAACCTTTGAGAGATATAATGTTTTAACTAGTTGAGCTATCCTTGGACTAAATCCAAACATATATAATGTCTTAACTTGTTGGTTTATGCTCGGGCTAAATCCATGTGTGTAAATTAGTACGTGACCCTAGTGATTATCCATATTCAATATCAATGGGAAAGAACGACATTGAACCTAAAAGTTCGAGTTGGTAGGTTATAGTAAACTTAATTGCTTATTCATTCATATTCTTGTATTTGCTCCTAGAGAGAGACTAATGGTAGAATAATTATAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCCACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGGTACACCCTTGTTGCCCTGATTATCGAAATTTATGTACACTCTTACTTGGAAGTGATTTGAGAAGGCATAACTTTGTGTCCGTGCATTTCTTTATGTGCAGCCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGAAGATCTGGACAGATGGTCTCTGGAAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAATTTCTTGACCAGTCAGTTGAACTTGAAAATGGACATATTAGTCACTTTGAGGTATTAATTCATGGGGATCTGTTGTTGTGATTGAAAAATCTCCAAGAACCCAAATGGCTTATATGCTGCTTCTACGTGATTAATTTAAGCAAAATGATGTTCCAACTAGATTTTCACTCTGTAAATTTGTCTGATGATCTTTTATTTCCCGCTGGATTTGGACTTCATACATGTTTGGTATATCCTGCATCCTTTTAGGCTTGATTTCATCTAGGAAGGCATAAATTGTTCCTGTCAACGTGCTAAGCCTTTTGGACCCCTTAAAAGTTCATTGGACCTATGCAGTCTTTAATGTTTCCTTTAATCTTTTAAACGAGCTGATGAAATTCTCCCAGGTTCTTACTGCTATGGCATTCTCACTCTTTGCTCAAGAGAAAGTTGATGTTGCAGTTATTGAGGTTCGCTTTCTTTTAGACTAATTTTTTCTTTGCTAAGTATTTTTTATTTCTCTAGCAAAAGCTTTTAACGCCATTGGCAATATGTTCTTATTTTAACTGAAACAGGCTGGACTTGGTGGTGCACGAGATGCAACCAACATAATTTGCAGCTCCAGACTTGCTGCTGCAGTCATAACTTCAATTGGGGAGGAACACATGGCTGCGCTGGGTGGCTCTTTAGAAAGCATTGCAACGGCAAAGGCTGGAATAATTAAACATGGTTGTCCAGTTAGGTTGTTTTCTTGATGTAATTTTGATGCTGATTGACTTTGTTGATCCTTCGATATCTATTATCCATGCATTAGTTAAGTTTTTCCTTTTCTAATGAGTTGACATGATCTACAAGAAGAGAAGAGGAAACTTGGAGTCAGCCTGAACTTACATAAGACAATGGAGATCACAAACATGCCCTCCAGTTAGCCTTAATTCTTGTTTCAAGTAATAGTAGTTGTTGAAAGATTAAATTGCAGTTTTTGTCATAATTAACTTTAGGCATAAGAAAACTGAATTGCAGTCTTTCTTTCAAATTATATTTGCCATATAGGAAGGTATCAAATTGGTTTATTGCTGCAAACGACCTAAAAGCAACCTGCATGAAAAGTTCTTAGAGACTTCCAAAGTCCATTCTCTGGTCTTCGGCGTCTAGGGGCATGGAGTTTTCTCTTCTAAAGTTGATGTAAATGTATGTGTTTGTGTGTCGGTGTCTATGTCTGAGAGAGAAGCTTATTCTCCTACTTCTATTGTGATAATATTGTCCTTTTAATTTATGTGATTTCTCTTCTGAAATTTATTCCTAAAATGCTCCTTACATCTGATTTCAGACCATTCTGGGTGGTCCTTTCCTTCCAAGCGTTGAGTGCATCCTTCGCGATAAGGCATTATCCATGTCTTCGTCTGTATTATCAGCCTCAGATCCTGGAAATAAAAGTACCATAAAAGGTGTAAGCCTGCTCAATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAAGTATGGCTTATGTATTCTATTAAAGAAATATAAATTTCACTCTTGAACTTGAACTTCTATCAAGAAGAGTTCCTTAATCTCATTGTCAATGCAGTTCATAGAGTTATTGGATGTCAACCTCCGCATGCTTGGAAGTCATCAACTTCAGAATGCAGCCACCGCAACTTGTGTTATTCTTACTCTCCGAAATTTAGGTATAATATCATTTTGCATATTTGATTTGATATCCTACCTTCATTTAGAGAATAAACATGTGGCGTGGTGTGATAATTTCCATGGCACGTTTACTGAAGATAATAGCCAGGATACTGTTCGCATGCAAAAACTCTGCTGACACTTTATATTAGTCTCTGACATTAGTGGTTCTGCTAAAGTATAAGGGAAAGATGTAGTTACTTATTATTGCGTGCTTTAGATGATACATGATGCAAGTTAAATTGTAGTTGATTTCACTGAATACTCTCTTTGTATTTTCCTAATCTGTTAAATAATTCAGGTTGGAGAATTTCAGACGCATCAATTAGGAGTGGACTAGAGCAAACATTCTTGGTTGGTAGGAGTCATTTCTTATCAGCTGAAGAAGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTCGATGGAGGTGCATTCATATTTCTTTCTATTAACGTCAAGTTTGGTCTATTGGGACCTGGGCATTGTGCACTTGCCGCGTCTCCTCTACCCTATTGTACTTAAGACTTTCGATAATTTGCTGTTTAGTATGCTGGTACTCTTGTAGCTGTTATTGGTAGATGTTTCTAGTTTATGGATTACTGATTGCTTAAAGATTTGCATAATGTTCTAGATCTTCTTAGGGTTAATCTAATGGAATTTAGCAGATAAAATTTCAGTTCATGTCAGCTGATATATAGTCGAGTTTTCGTAGTTCTGTTCTGGTCTGGATGCTAGTTCCATGTCTTACATTCTTTTAGACAGCATTTCTGATTGTTGCTTGAATGTTTTCTCAGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGCCACCATTCAGATGACTTTTCCAGAGGCACGATTGGCTCTTGTGGTTGCAATGGCTAGTGATAAGGACCACATTGGTTTTGCCAGAGAATTTCTTAAAGGTATAGCCTTCCCCTTCACCGAGTTTGAAACTGAAGATGAAGAATATTGCTTGATTGTAAAATTTTCTATAGTCGTGAAAATTTTGGATTATATAGGGCTCGTTTGATAGCCACGTTGCTTTTAGATCATATGAGTGTGTGTAGAGATAGGTTATCTTAGATTGAAATAGATGACCAAAAGAAAGTGTGTAAAGAGAAGTTGGCAAGAGAAAATACATGTTTAAATAGAGTAAATACACTAGAACTGTTAGCTTTAGTTTTGTGTAATTTGGTTTTAGAAATTTGTGTTTTTTTTTTCTTAAATATTAGTTTGAGTTTCAAAATTTTTATTAATCAAAGCTTAAAAAACAAAAACAGAAACACAATTATCTTCTTTATATCTTGGGCAGGAGGCCTAAGAGAGGTCTGAAGTCCTTTTATCAAATTACAGGCTTCCTATTCTCTCTAATTCTGATTTATTTTTTTTTAACAGGACTCGAAAACTTTCATTGAAGTAGTGAAAAGAAATCGTTGTTTAGAGAAAAAGAATTAAACTAAACGAGGTCTATGAAAGCACCCTAATTAATACAAATATCCCGAGAAGCGTAATGAAAACACTTTAGAAAGAGAACACCGTCGAGAAGCTTTTAATATAGAAATATCTTGTTGTTTTTCTATATCTTCTCTAATGTTGTAAGAGGTAGCAATGAAATGCTGAAAGTCAGCAGTTAACTGAATTTCAGGTGGAAAATTGGAGTCCATTGTCTTAACTGAAGCCAATATTGCTGGAGCCAAATCAAGGACAACTTCAGCTGCTCTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGCATCCCTATTTCTCTAGAAACTAAAGATGCATCGGTTTCCTCCACGAGCAAACTCGAAAACAGGCCGATATTGACTACCGAGGCCTCGTTATTACATGCCATAAAAATCACTGCAGAGAATCTCAAGCAGAGAACGGAAGGGCAGCAACAAGGCCTAATCGTAGTAACTGGATCGCTGCATGCGGTTTCAATGGTGTTATCTTCTATTCATTCTTGAAGTCTGTACTTATGAAAGCACTTCATTTTGATGTGTACTTTAAGCAAAAGTTCATGAGTGTTACAGATTCTTTTGGGTAGAATATTTCCTATGAATTCCTTGATATGTAAGATTAATGGCAGAGAAAAGCAATGAAAGTTGGTTCCAATTTTGGCCAGAATCCATGATTGTTGG

mRNA sequence

ATGAATATCTTCAAACATCATCACCACTGTCGCCCACAGATTCTCGGAAGGCTTCTCCTAAATTATTTCGCTGGTGAAAGGCCTTCTATCAATCGAATAATAGGGTCCAAACAATGCTTCGGCACTCACTCAGAAGATCAAGAGATGACGGATTTCATGGAATATTTAGATTCCCTGAAGAATTATGAGAAATTGGGTGTGCCCAGAGGCGCAGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGCTGATGGAGCGGCTGGGCAATCCACAATCGAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCCACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGCCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGAAGATCTGGACAGATGGTCTCTGGAAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAATTTCTTGACCAGTCAGTTGAACTTGAAAATGGACATATTAGTCACTTTGAGGTTCTTACTGCTATGGCATTCTCACTCTTTGCTCAAGAGAAAGTTGATGTTGCAGTTATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACCAACATAATTTGCAGCTCCAGACTTGCTGCTGCAGTCATAACTTCAATTGGGGAGGAACACATGGCTGCGCTGGGTGGCTCTTTAGAAAGCATTGCAACGGCAAAGGCTGGAATAATTAAACATGGTTGTCCAACCATTCTGGGTGGTCCTTTCCTTCCAAGCGTTGAGTGCATCCTTCGCGATAAGGCATTATCCATGTCTTCGTCTGTATTATCAGCCTCAGATCCTGGAAATAAAAGTACCATAAAAGGTGTAAGCCTGCTCAATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCATAGAGTTATTGGATGTCAACCTCCGCATGCTTGGAAGTCATCAACTTCAGAATGCAGCCACCGCAACTTGTGTTATTCTTACTCTCCGAAATTTAGGTTGGAGAATTTCAGACGCATCAATTAGGAGTGGACTAGAGCAAACATTCTTGGTTGGTAGGAGTCATTTCTTATCAGCTGAAGAAGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTCGATGGAGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGCCACCATTCAGATGACTTTTCCAGAGGCACGATTGGCTCTTGTGGTTGCAATGGCTAGTGATAAGGACCACATTGGTTTTGCCAGAGAATTTCTTAAAGGTGGAAAATTGGAGTCCATTGTCTTAACTGAAGCCAATATTGCTGGAGCCAAATCAAGGACAACTTCAGCTGCTCTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGCATCCCTATTTCTCTAGAAACTAAAGATGCATCGGTTTCCTCCACGAGCAAACTCGAAAACAGGCCGATATTGACTACCGAGGCCTCGTTATTACATGCCATAAAAATCACTGCAGAGAATCTCAAGCAGAGAACGGAAGGGCAGCAACAAGGCCTAATCGTAGTAACTGGATCGCTGCATGCGGTTTCAATGGTGTTATCTTCTATTCATTCTTGA

Coding sequence (CDS)

ATGAATATCTTCAAACATCATCACCACTGTCGCCCACAGATTCTCGGAAGGCTTCTCCTAAATTATTTCGCTGGTGAAAGGCCTTCTATCAATCGAATAATAGGGTCCAAACAATGCTTCGGCACTCACTCAGAAGATCAAGAGATGACGGATTTCATGGAATATTTAGATTCCCTGAAGAATTATGAGAAATTGGGTGTGCCCAGAGGCGCAGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGGCTGATGGAGCGGCTGGGCAATCCACAATCGAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCCACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGCCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGAAGATCTGGACAGATGGTCTCTGGAAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAATTTCTTGACCAGTCAGTTGAACTTGAAAATGGACATATTAGTCACTTTGAGGTTCTTACTGCTATGGCATTCTCACTCTTTGCTCAAGAGAAAGTTGATGTTGCAGTTATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACCAACATAATTTGCAGCTCCAGACTTGCTGCTGCAGTCATAACTTCAATTGGGGAGGAACACATGGCTGCGCTGGGTGGCTCTTTAGAAAGCATTGCAACGGCAAAGGCTGGAATAATTAAACATGGTTGTCCAACCATTCTGGGTGGTCCTTTCCTTCCAAGCGTTGAGTGCATCCTTCGCGATAAGGCATTATCCATGTCTTCGTCTGTATTATCAGCCTCAGATCCTGGAAATAAAAGTACCATAAAAGGTGTAAGCCTGCTCAATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCATAGAGTTATTGGATGTCAACCTCCGCATGCTTGGAAGTCATCAACTTCAGAATGCAGCCACCGCAACTTGTGTTATTCTTACTCTCCGAAATTTAGGTTGGAGAATTTCAGACGCATCAATTAGGAGTGGACTAGAGCAAACATTCTTGGTTGGTAGGAGTCATTTCTTATCAGCTGAAGAAGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTCGATGGAGCCCACACCAAAGACTCTGCTAAAGCATTAGTGGCCACCATTCAGATGACTTTTCCAGAGGCACGATTGGCTCTTGTGGTTGCAATGGCTAGTGATAAGGACCACATTGGTTTTGCCAGAGAATTTCTTAAAGGTGGAAAATTGGAGTCCATTGTCTTAACTGAAGCCAATATTGCTGGAGCCAAATCAAGGACAACTTCAGCTGCTCTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGCATCCCTATTTCTCTAGAAACTAAAGATGCATCGGTTTCCTCCACGAGCAAACTCGAAAACAGGCCGATATTGACTACCGAGGCCTCGTTATTACATGCCATAAAAATCACTGCAGAGAATCTCAAGCAGAGAACGGAAGGGCAGCAACAAGGCCTAATCGTAGTAACTGGATCGCTGCATGCGGTTTCAATGGTGTTATCTTCTATTCATTCTTGA

Protein sequence

MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSLLNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLALVVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIPISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAVSMVLSSIHS

Homology

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BLAST of Spg002568 vs. NCBI nr
Match: XP_022141068.1 (dihydrofolate synthetase isoform X1 [Momordica charantia])

HSP 1 Score: 954.5 bits (2466), Expect = 3.9e-274
Identity = 494/549 (89.98%), Postives = 519/549 (94.54%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+H   RPQILG LLLN+  GE PSI+  IGSKQCF THSED E+T+FMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSG+MVSGKALNSLFKRNKEFLDQSVE ENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIKHGCPTILGGPFLPS+ECIL DKALSMSS V+SAS PG++STIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           L+GRLCQCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           ++GLEQTFLVGRSHFLSA+EAGVLGLPGATILLDGAHTKDSAKALV TIQMTFPEARLAL
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH+GFAREFL+GGKLESI+LTEANI G KSRTTSAA+LRDCWIQA+NELGIP
Sbjct: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VSSTSKLE+RPILTTE SLLHA+KI AE LKQRTEG + GL VVTGSLHAV
Sbjct: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEG-RAGLTVVTGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 548

[Close]

BLAST of Spg002568 vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 951.0 bits (2457), Expect = 4.4e-273
Identity = 488/549 (88.89%), Postives = 519/549 (94.54%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+HHH RPQI GRLLLNYF GE PSI+  IGSKQCFG HSEDQ+MT+FMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIKHGCPTILGGPF+P++ECILRDKALSMSS V+SASDPGN+STIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGRLCQCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLE+TFLVGRSHFLSA+EAG+LGLPG TILLDGAHTKDSAKALV TIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+GGKLESIVL+EANI G KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VSSTSKLENRP+LTTE SLL AIKI AE LKQR EG ++GL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIEG-RRGLVVVTGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 548

[Close]

BLAST of Spg002568 vs. NCBI nr
Match: KAG6576673.1 (Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 948.3 bits (2450), Expect = 2.8e-272
Identity = 485/549 (88.34%), Postives = 518/549 (94.35%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+HHH RPQI GRLLLNYF GE PSI+  IGSKQCFG HSEDQ+MT+FMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIKHGCPTILGGPF+P++ECILRDKALSMSS V+SASDPGN+STIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGRLCQCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLE+TFLVGRSHFLSA+EAG+LGLPG  ILLDGAHTKDSAKALV TIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+GGKLESIVL+EANI G KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VSSTSKLENRP+LTTE SLL AIKI AE LKQR +G ++GL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDG-RRGLVVVTGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 548

[Close]

BLAST of Spg002568 vs. NCBI nr
Match: XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])

HSP 1 Score: 947.6 bits (2448), Expect = 4.8e-272
Identity = 490/549 (89.25%), Postives = 512/549 (93.26%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           M IFK+HHHCRPQILG LLLN+F GE PSI+  IGSKQCF TH EDQ MT+FMEYLDSLK
Sbjct: 1   MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP+GAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYS GCYTSPHIETIRERISLGRSG+MVSGKALNSLFKRNKEF DQSVELENG ISH
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAF LFAQE VD+AVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIATAKAGIIK GCP ILGGPFLPS+E ILRDKALSMSS V+SAS PGN+STIKGVSL
Sbjct: 241 LESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVISASGPGNRSTIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNG +CQCCD+VIQID+EFIELLDVNLRMLG HQLQNAATATCVIL LRNLGWRISDAS+
Sbjct: 301 LNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCVILALRNLGWRISDASV 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLEQTFLVGRSHFL A+EAGVLGLPGATILLDGAHTKDSAKALV TIQM FPEARLAL
Sbjct: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDHIGFAREFL+GGKLESIVLTEA+I G KSRTTSA  LRDCWIQASNELGIP
Sbjct: 421 VVAMASDKDHIGFAREFLQGGKLESIVLTEAHIGGGKSRTTSAVFLRDCWIQASNELGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VS TSKLENRP+LTTE SLLHAIK+ AE LKQRTEG +QGL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSFTSKLENRPVLTTETSLLHAIKVAAEILKQRTEG-RQGLVVVTGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLS +HS
Sbjct: 541 SMVLSFLHS 548

[Close]

BLAST of Spg002568 vs. NCBI nr
Match: XP_022922898.1 (dihydrofolate synthetase isoform X1 [Cucurbita moschata])

HSP 1 Score: 945.7 bits (2443), Expect = 1.8e-271
Identity = 484/549 (88.16%), Postives = 518/549 (94.35%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+HHH RPQI GRLLLNYF GE PSI+  IGSKQCF  HSEDQ+MT+FMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIKHGCPTILGGPF+P++ECILRDKALSMSS V+SASDPGN+S+IKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGRLCQCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLE+TFLVGRSHFLSA+EAG+LGLPG  ILLDGAHTKDSAKALV TIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+GGKLESIVL+EANI G+KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VSSTSKLENRP+LTTE SLL AIKI AE LKQR +G ++GL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDG-RRGLVVVTGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 548

[Close]

BLAST of Spg002568 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 7.5e-159
Identity = 307/509 (60.31%), Postives = 383/509 (75.25%), Query Frame = 0


Query: 44  SEDQEMTDFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED E+ DF+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +G+ VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +E VD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPFLP +E ILR KA S+SSSV
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 LSASDPGNKSTIKGVSLLNG-RLCQCCDIVIQ---IDNEFIELLDVNLRMLGSHQLQNAA 343
           + AS+ G+ S+IKG+   NG  LCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCVILTLRNLG-WRISDASIRSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHT 403
           TATCV L LR+ G  R++D +IR GLE T L+GRS FL+ +EA  L LPGAT+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399

Query: 404 KDSAKALVATIQMTFPEARLALVVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKS 463
           K+SA+AL   I+  FPE RL  VVAMASDKDH+ FA+E L G K E+++LTEA+I G K 
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459

Query: 464 RTTSAALLRDCWIQASNELGIPISLETKDASVSSTSKLENRPILTTEASLLHAIKITAEN 523
           R+T +++L++ WI+A++ELG      + +AS + T              +L ++K+  + 
Sbjct: 460 RSTESSVLKESWIKAADELG----SRSMEASENKT--------------VLGSLKLAYKI 519

Query: 524 LKQRTEGQQQGLIVVTGSLHAVSMVLSSI 548
           L   T     G+++VTGSLH VS VL+S+
Sbjct: 520 LSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of Spg002568 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 180.3 bits (456), Expect = 6.1e-44
Identity = 122/356 (34.27%), Postives = 188/356 (52.81%), Query Frame = 0


Query: 81  LGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRM++LM RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQ-EKVDV 200
           RIS+  +G  +S +   +L  + K  ++   + E G  + FE++TA AF  FA+  KVD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 201 AVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPTI 260
            + E GLGG  D+TN++        VITSIG +HM  LG ++E IA  KAGIIK G P I
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIP-I 203

Query: 261 LGGPFLPSVECILRDKALSMSSSVLSASDP---GNKSTIKGVSLLNGRLCQCCDIVIQID 320
           +     P    ++R +A   ++   S  D     N+  +      + +  + C       
Sbjct: 204 VTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGEQFSFKTEEKC------- 263

Query: 321 NEFIELLDVNLRMLGSHQLQNAATATCVI--LTLRNLGWRISDASIRSGLEQTFLVGRSH 380
                  D+   ++G+HQ QNAA +      L   N+   ISD ++RSGL +    GR  
Sbjct: 264 -----YEDIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGRLE 323

Query: 381 FLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLALVVAMASDKDH 431
            +              + LDGAH ++  + L  T++  F  +R+++V +   DK +
Sbjct: 324 LVQEH---------PPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPY 351

[Close]

BLAST of Spg002568 vs. ExPASy Swiss-Prot
Match: D4GW05 (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=folCP PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 6.3e-33
Identity = 111/348 (31.90%), Postives = 168/348 (48.28%), Query Frame = 0


Query: 84  MRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERIS 143
           +RRL+  LG+P      + +AG+ GKGSTA  +  +LR  G  VG YTSPH + +RER+ 
Sbjct: 25  IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVRERVR 84

Query: 144 LGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIE 203
           +   G+ +   AL++     K +L +    +   ++ FE +TA+A   F +  VDVAV+E
Sbjct: 85  V--DGRKIPKSALSAFVAEAKPYLVERA-ADGEPLTFFETVTALALWYFDRAGVDVAVLE 144

Query: 204 AGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPTILGGP 263
            G+GG  DAT+ +      A+ +T++  EH A LG ++  IA  KA +     P + G  
Sbjct: 145 VGMGGELDATSAVDP---VASAVTNVSLEHTAVLGDTVAEIAKTKAAVAPADAPLVTG-- 204

Query: 264 FLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSLLNGRL-CQCCDIVIQIDNEFIEL 323
                  ++R++A      VL+  D    S         GR+  Q   + ++ D    E 
Sbjct: 205 TTGEALSVIREEA----GDVLTVGDADADSDADVRVSYGGRVNHQEAAVTVETD---AET 264

Query: 324 LDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLVGRSHFLSAEEAG 383
           LDV + +LG++Q +NA  A  +   +R     I   +I  GL      GR   +  E   
Sbjct: 265 LDVRIPLLGAYQARNAGIAVSLARQVRP---DIDAEAIHRGLRNAHWPGRFEVMGTE--- 324

Query: 384 VLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLALVVAMASDKDH 431
                  T++LDGAH  D A A VAT+   F    L LV     DKDH
Sbjct: 325 ------PTVVLDGAHNPD-ACAQVATVLDEFDYDDLHLVYGAMHDKDH 344

[Close]

BLAST of Spg002568 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 7.7e-31
Identity = 101/357 (28.29%), Postives = 173/357 (48.46%), Query Frame = 0


Query: 80  DLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIR 139
           D  R+  L+  LGNPQ + + IH+ GT GKGS A  ++++L A G +VG YTSP I    
Sbjct: 21  DHRRILTLLHALGNPQQQGRYIHVTGTNGKGSAANAIAHVLEASGLTVGLYTSPFIMRFN 80

Query: 140 ERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELE-NGHISHFEVLTAMAFSLFAQEKVD 199
           ERI +    + +   AL +     +  L++  + + + +++ FE +TA+ +  F Q +VD
Sbjct: 81  ERIMIDH--EPIPDAALVNAVAFVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVD 140

Query: 200 VAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPT 259
           VAVIE G+GG  D+TN+I       +V+T +  +H   LG ++ +IA  KAGIIK G P 
Sbjct: 141 VAVIEVGIGGDTDSTNVITP---VVSVLTEVALDHQKLLGHTITAIAKHKAGIIKRGIPV 200

Query: 260 ILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSLLNGRLCQCCDIVIQIDNE 319
           + G   +P    ++  K  +  S  L           +  S+   +L          D +
Sbjct: 201 VTGN-LVPDAAAVVAAKVATTGSQWLRFD--------RDFSVPKAKLHGWGQRFTYEDQD 260

Query: 320 FIELLDVNLRMLGSHQLQNAATA-TCVILTLRNLGWRISDASIRSGLEQTFLVGRSHFLS 379
              + D+ + ++G +Q +N A A     +  +   W ++  +IR GL  +    R   +S
Sbjct: 261 G-RISDLEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARLEKIS 320

Query: 380 AEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLALVVAMASDKDHIGFA 435
                        I++DGAH  D    L+  ++  F +  + ++  + +DKD+   A
Sbjct: 321 ---------DTPLIVIDGAHNPDGINGLITALKQLFSQP-ITVIAGILADKDYAAMA 352

[Close]

BLAST of Spg002568 vs. ExPASy Swiss-Prot
Match: I6Y0R5 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=folC PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.5e-29
Identity = 113/363 (31.13%), Postives = 172/363 (47.38%), Query Frame = 0


Query: 81  LGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           L R+  LM+ LG+PQ  + +IHIAGT GK S A  +  ++ A     G  TSPH+++  E
Sbjct: 47  LTRISALMDLLGSPQRSYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVE 106

Query: 141 RISLGRSGQMVSGKALNSLFKRNK---EFLDQSVELENGH----ISHFEVLTAMAFSLFA 200
           RIS+   G+ +S     + ++  +     +DQ  +   G     +S FEVLTAMAF+ FA
Sbjct: 107 RISI--DGKPISPAQYVATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAFAAFA 166

Query: 201 QEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIK 260
              VDVAV+E G+GG  DATN+I       AVIT I  +H+  LG  +  IA  KAGII 
Sbjct: 167 DAPVDVAVVEVGMGGRWDATNVI---NAPVAVITPISIDHVDYLGADIAGIAGEKAGIIT 226

Query: 261 H---GCPTILG--GPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSLLNGRLCQC 320
               G P  +   G  +P V  +L  +++   +SV  A +    + ++    + G+    
Sbjct: 227 RAPDGSPDTVAVIGRQVPKVMEVLLAESVRADASV--AREDSEFAVLRRQIAVGGQ---- 286

Query: 321 CDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLG--WRISDASIRSGLEQ 380
              V+Q+        D+ L + G HQ  NA  A   +      G   ++   ++R+G   
Sbjct: 287 ---VLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGAGAQRQLDGDAVRAGFAA 346

Query: 381 TFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLALVVAMAS 430
               GR   + +           T+ +D AH    A AL  T+   F    L  V+++  
Sbjct: 347 VTSPGRLERMRS---------APTVFIDAAHNPAGASALAQTLAHEFDFRFLVGVLSVLG 386

[Close]

BLAST of Spg002568 vs. ExPASy TrEMBL
Match: A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 494/549 (89.98%), Postives = 519/549 (94.54%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+H   RPQILG LLLN+  GE PSI+  IGSKQCF THSED E+T+FMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSG+MVSGKALNSLFKRNKEFLDQSVE ENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIKHGCPTILGGPFLPS+ECIL DKALSMSS V+SAS PG++STIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           L+GRLCQCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           ++GLEQTFLVGRSHFLSA+EAGVLGLPGATILLDGAHTKDSAKALV TIQMTFPEARLAL
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH+GFAREFL+GGKLESI+LTEANI G KSRTTSAA+LRDCWIQA+NELGIP
Sbjct: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VSSTSKLE+RPILTTE SLLHA+KI AE LKQRTEG + GL VVTGSLHAV
Sbjct: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEG-RAGLTVVTGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 548

[Close]

BLAST of Spg002568 vs. ExPASy TrEMBL
Match: A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 8.9e-272
Identity = 484/549 (88.16%), Postives = 518/549 (94.35%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+HHH RPQI GRLLLNYF GE PSI+  IGSKQCF  HSEDQ+MT+FMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIKHGCPTILGGPF+P++ECILRDKALSMSS V+SASDPGN+S+IKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGRLCQCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRIS+ASI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLE+TFLVGRSHFLSA+EAG+LGLPG  ILLDGAHTKDSAKALV TIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+GGKLESIVL+EANI G+KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VSSTSKLENRP+LTTE SLL AIKI AE LKQR +G ++GL+VVTGSLHAV
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDG-RRGLVVVTGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 548

[Close]

BLAST of Spg002568 vs. ExPASy TrEMBL
Match: A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 8.3e-270
Identity = 484/549 (88.16%), Postives = 511/549 (93.08%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+HHHCRPQILG LLLN+F GE PSI+   GSKQCF THSEDQ MT FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           R EGYSVGCYTSPHIETIRERISLGRSG MVSGKALNSLFKRNKE  DQSVELENGH+SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIATAKAGIIK GCPTILGGPFLP +E ILRDKALSMSS V+SASDPGN+STIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNG L QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLEQTFL+GRSHFL+A EA VLGLPGATILLDGAHTKDSAKAL+ TIQM FPEA+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDK+H+GFAREFL+GGKLES+VLTEA I G KSRTTSAA LRDCWIQASNELGIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA V  TSKL NRP+LTTE SLLHAIKI AE LKQRT+G +QGL+VV+GSLHAV
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKG-RQGLVVVSGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVL+S+HS
Sbjct: 541 SMVLASLHS 548

[Close]

BLAST of Spg002568 vs. ExPASy TrEMBL
Match: A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.5e-268
Identity = 481/549 (87.61%), Postives = 510/549 (92.90%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+HHHCRPQILG LLLN+F GE PSI+   GSKQCF THSEDQ MT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKE  DQSVELE+G ISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIK GCPTILGG FLP +E ILRDKALSMSS V++ SDPGN+STIKGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNG LCQCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLEQTFL+GRSHFL+A+EA VLGLPGATILLDGAHTKDSAKALV TIQM FPEARLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           V+AMASDK+H+ FAREFL+GGKLE +VLTEA+I G KSRTTSAA LRDCWIQAS ELGIP
Sbjct: 421 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
           ISLETKDA VS TSKLENRP+LTTE SLLHAIKI AE LKQRT+G QQ L+VV+GSLHAV
Sbjct: 481 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKG-QQSLVVVSGSLHAV 540

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 548

[Close]

BLAST of Spg002568 vs. ExPASy TrEMBL
Match: A0A6J1J6Z7 (dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.5e-258
Identity = 470/549 (85.61%), Postives = 502/549 (91.44%), Query Frame = 0


Query: 1   MNIFKHHHHCRPQILGRLLLNYFAGERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLK 60
           MN+FK+HHH R QILGRLLLNYF GE PSI+  IGSKQCFG HSEDQ+MT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHFRLQILGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRNKEFLDQSVELENGHISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE LDQSV+LENG ISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSILAAAVITSIGEEHMAALGGS 240

Query: 241 LESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSVLSASDPGNKSTIKGVSL 300
           LESIA AKAGIIKHGCPTILGGPF+P++ECILRDKALSMSS V+SASDPGN+STIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LNGRLCQCCDIVIQIDNEFIELLDVNLRMLGSHQLQNAATATCVILTLRNLGWRISDASI 360
           LNGRLCQCCD++IQ D+EFIEL DVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGRLCQCCDLIIQTDDEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 RSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHTKDSAKALVATIQMTFPEARLAL 420
           RSGLE+TFLVGRSHFLSA+EAG+LGLPG TILLDGAHTKDSAKALV TIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKSRTTSAALLRDCWIQASNELGIP 480
           VVAMASDKDH GFA EFL+ GKLESIVL+EANI G KSRTTSAALLRDCWIQASNE+GIP
Sbjct: 421 VVAMASDKDHNGFATEFLQCGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDASVSSTSKLENRPILTTEASLLHAIKITAENLKQRTEGQQQGLIVVTGSLHAV 540
                     + TS      +LTTE SLL AIKI AE LKQRTEG ++GL+VVTGSLHAV
Sbjct: 481 ----------TQTS------LLTTETSLLRAIKIAAEILKQRTEG-RRGLVVVTGSLHAV 532

Query: 541 SMVLSSIHS 550
           SMVLSS+HS
Sbjct: 541 SMVLSSLHS 532

[Close]

BLAST of Spg002568 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 562.0 bits (1447), Expect = 5.3e-160
Identity = 307/509 (60.31%), Postives = 383/509 (75.25%), Query Frame = 0


Query: 44  SEDQEMTDFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED E+ DF+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +G+ VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNIICSSRLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +E VD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIATAKAGIIKHGCPTILGGPFLPSVECILRDKALSMSSSV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPFLP +E ILR KA S+SSSV
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 LSASDPGNKSTIKGVSLLNG-RLCQCCDIVIQ---IDNEFIELLDVNLRMLGSHQLQNAA 343
           + AS+ G+ S+IKG+   NG  LCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCVILTLRNLG-WRISDASIRSGLEQTFLVGRSHFLSAEEAGVLGLPGATILLDGAHT 403
           TATCV L LR+ G  R++D +IR GLE T L+GRS FL+ +EA  L LPGAT+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399

Query: 404 KDSAKALVATIQMTFPEARLALVVAMASDKDHIGFAREFLKGGKLESIVLTEANIAGAKS 463
           K+SA+AL   I+  FPE RL  VVAMASDKDH+ FA+E L G K E+++LTEA+I G K 
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459

Query: 464 RTTSAALLRDCWIQASNELGIPISLETKDASVSSTSKLENRPILTTEASLLHAIKITAEN 523
           R+T +++L++ WI+A++ELG      + +AS + T              +L ++K+  + 
Sbjct: 460 RSTESSVLKESWIKAADELG----SRSMEASENKT--------------VLGSLKLAYKI 519

Query: 524 LKQRTEGQQQGLIVVTGSLHAVSMVLSSI 548
           L   T     G+++VTGSLH VS VL+S+
Sbjct: 520 LSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of Spg002568 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-27
Identity = 100/322 (31.06%), Postives = 151/322 (46.89%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E+VDV ++E GLGG  DATN+
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNV 175

Query: 216 ICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPTILGGPFLPSVECILRDK 275
           I   +     I+S+G +HM  LG +L  IA  KAGI K G P                  
Sbjct: 176 I--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP------------------ 235

Query: 276 ALSMSSSVLSASDPGNKSTIKGVSLLNGRLCQCCDIVIQIDNEFIELLDVNLR--MLGSH 335
                    + + P      + + +LN +  +     ++++ + +E LD + R  + G H
Sbjct: 236 -------AFTVAQPD-----EAMRVLNEKASK-----LEVNLQVVEPLDSSQRLGLQGEH 295

Query: 336 QLQNAATATCVILT-LRNLGWRISDA---------SIRSGLEQTFLVGRSHFLSAEEAGV 395
           Q  NA  A  +  T L+ +G    +             SGL   +L+GR+  +   E   
Sbjct: 296 QYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE--- 333

Query: 396 LGLPGATI-LLDGAHTKDSAKA 405
             LP   +  LDGAH+ +S +A
Sbjct: 356 --LPEEIVYYLDGAHSPESMEA 333

[Close]

BLAST of Spg002568 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 120.6 bits (301), Expect = 4.1e-27
Identity = 100/322 (31.06%), Postives = 150/322 (46.58%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E+VDV ++E GLGG  DATNI
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNI 175

Query: 216 ICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCPTILGGPFLPSVECILRDK 275
               +     I+S+G +HM  LG +L  IA  KAGI K G P                  
Sbjct: 176 ---QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP------------------ 235

Query: 276 ALSMSSSVLSASDPGNKSTIKGVSLLNGRLCQCCDIVIQIDNEFIELLDVNLR--MLGSH 335
                    + + P      + + +LN +  +     ++++ + +E LD + R  + G H
Sbjct: 236 -------AFTVAQPD-----EAMRVLNEKASK-----LEVNLQVVEPLDSSQRLGLQGEH 295

Query: 336 QLQNAATATCVILT-LRNLGWRISDA---------SIRSGLEQTFLVGRSHFLSAEEAGV 395
           Q  NA  A  +  T L+ +G    +             SGL   +L+GR+  +   E   
Sbjct: 296 QYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE--- 332

Query: 396 LGLPGATI-LLDGAHTKDSAKA 405
             LP   +  LDGAH+ +S +A
Sbjct: 356 --LPEEIVYYLDGAHSPESMEA 332

[Close]

BLAST of Spg002568 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 111.3 bits (277), Expect = 2.5e-24
Identity = 82/232 (35.34%), Postives = 116/232 (50.00%), Query Frame = 0


Query: 26  ERPSINRIIGSKQCFGTHSEDQEMTDFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMR 85
           E+ +IN  +GSK+    +       D ME L +L +    G       + D    +    
Sbjct: 62  EKAAIN--MGSKEDKADNPALSSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYL 121

Query: 86  RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLG 145
           ++++ L +   + K IH+AGTKGKGST  F   ILR  G+  G +TSPH+  +RER  + 
Sbjct: 122 KILD-LEDKIKELKVIHVAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRI- 181

Query: 146 RSGQMVSGKALNSLFKRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAG 205
             G  +S +     F    + L +           F+ LT +AF +F  EKVDVAVIE G
Sbjct: 182 -DGLDISEEKFLQYFWECWKLLKEKAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVG 241

Query: 206 LGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCP 258
           LGG  D+TN+I   +     I S+G +HM  LG +L  IA  KAGI K   P
Sbjct: 242 LGGKLDSTNVI--QKPVVCGIASLGMDHMDILGNTLADIAFHKAGIFKPQIP 286

[Close]

BLAST of Spg002568 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 111.3 bits (277), Expect = 2.5e-24
Identity = 67/165 (40.61%), Postives = 93/165 (56.36%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGQMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 170

Query: 157 NSLF----KRNKEFLDQSVELENGHISHFEVLTAMAFSLFAQEKVDVAVIEAGLGGARDA 216
              F     R KE  ++ + +     ++F  L  +AF +FA E+VD A++E GLGG  DA
Sbjct: 171 LGYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDA 230

Query: 217 TNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKHGCP 258
           TN +   +     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 231 TNAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 267

The following BLAST results are available for this feature:

BLAST of Spg002568 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141068.1	3.9e-274	89.98	dihydrofolate synthetase isoform X1 [Momordica charantia]	[more]
XP_023553540.1	4.4e-273	88.89	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]
KAG6576673.1	2.8e-272	88.34	Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038905573.1	4.8e-272	89.25	dihydrofolate synthetase [Benincasa hispida]	[more]
XP_022922898.1	1.8e-271	88.16	dihydrofolate synthetase isoform X1 [Cucurbita moschata]	[more]

BLAST of Spg002568 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	7.5e-159	60.31	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	6.1e-44	34.27	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
D4GW05	6.3e-33	31.90	Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha...	[more]
P15925	7.7e-31	28.29	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]
I6Y0R5	2.5e-29	31.13	Dihydrofolate synthase/folylpolyglutamate synthase OS=Mycobacterium tuberculosis...	[more]

BLAST of Spg002568 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJG1	1.9e-274	89.98	dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115...	[more]
A0A6J1E819	8.9e-272	88.16	dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A0A0L7A4	8.3e-270	88.16	Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454...	[more]
A0A1S3CNR7	4.5e-268	87.61	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3...	[more]
A0A6J1J6Z7	2.5e-258	85.61	dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 ...	[more]

BLAST of Spg002568 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	5.3e-160	60.31	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	1.4e-27	31.06	DHFS-FPGS homolog D	[more]
AT3G55630.2	4.1e-27	31.06	DHFS-FPGS homolog D	[more]
AT3G10160.1	2.5e-24	35.34	DHFS-FPGS homolog C	[more]
AT5G05980.1	2.5e-24	40.61	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 379..549 e-value: 1.5E-17 score: 66.1
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 385..547
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 81..545 e-value: 2.8E-92 score: 308.1
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..545
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 103..344 e-value: 2.9E-10 score: 40.4
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 39..368 e-value: 2.7E-86 score: 291.2
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 78..368
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..545
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 201..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002568.1	Spg002568.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006761	dihydrofolate biosynthetic process
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008841	dihydrofolate synthase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity
molecular_function	GO:0016874	ligase activity