Spg002527 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002527
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Prolyl-tRNA synthetase
Location	scaffold6: 1340762 .. 1345645 (-)
RNA-Seq Expression	Spg002527
Synteny	Spg002527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAGTGTCTCTGAGACTACCTTCGCTCTCCTCTTTGTTTTCCCAAGCCTCACCTCGCTCCTCAAGCTTCACCTTCTCCAGGCGAGCTCTGGGGATTTCATGGCGCCAGCAACGCCCGTCCCGGTTGCCGCTTCTTGCTTCTGGATTCTCCGCGCGAAGTTCGTCCACTGGGACCGAAATCCGTGTCGACAACTCGAAGAATCGAGTTCCGGAAGGAGCCATCACGCCTCGGTCTCAGGACTTCAATGCTTGGTATTTGGATGTCATTGCTAATGCTGAGCTTGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCTTATGGCTACGCAATTTGGGAAGCAATACAGGTGGTGGAAGCTATATTTTCTGTTTATTTTGAGTTGAACATTTGAGCAGTGTATTTGAGCTTTTCTTGTTGTGTGTATAATCGGATGATTGTTCTTTCTGTAGGAATATTTGAATGTTAAGTTCAAGGAGACCGGTCATCAGAATATGTACTTTCCACAGGTGCGGTTCTGGCGTTTTCAAGAGTCATAATATAAATGAATATATGCATGCTAGAAATGTGAAATGTTGAAATCTTTGATTTTGACTCCAGTTTATACCATACTCGTTCATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCGGAGTTAGCTCTTGTAACAATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTACGTAGACTCAGCAAAGCTATTGGCCCTTTTCCAGTCTTTCATTACTCATTAGAGAAGATATTAACTTTATTATGTGTGATACTTTGCATCGGCTAAATTGGAAATGGATATTAGTCCTCCATAACGGTGACCTGAGGTTTGGTTTTGATTGTTGGGTTGCCTGAGGCTTTGGCAGGTTCGACCTACTAGTGAAACCATTGTGAATCACATGTTTACCCAGTGGATTCATAGTTACCGTGATCTTCCTTTGATGATTAATCAGGTTCGATGATTTTCTACATTATGAAAAATTCTCCCATTATCTTCGGCCTTTATGTTATCCTTATTCTATATCTTTTCTTTTGTGTATTTGGTGTAGTGGGCGAATGTTACTAGATGGGAGATGCGCACGAAACCGTTTGTAAGAACTCTTGAATTTCTCTGGCAGGAGGGTCATACTGCTCATGTCAGTCCAGAAGAGGCAGAAAAAGAGGTAGTTATCTGATGCATCCAAAAGACTACTTTATCTTGTTTCCTTTTTTAGTCGCATACTTTTCAGTTAAGAACTTTGATGCGGCTTTGCAGGCTATTCAGATGATTGACATATATACAAAATTTGCTTATGAGCAAGCGGCAATACCTGTTATTGCAGGTCGAAAATCAAAGGTGGAGACATTTGCTGGTGCAGACAAGACCTATACAATAGAGGCCATGATGGGGGATCGAAAAGCACTGCAGGCAGGAACCAGCCACAACCTTGGGCAAAACTTTTCTCGTGCTTTTGGAACACAGGTATTTTGACAAATCAACTAAATTTCTTCCGACTTACATGCAGTCATGCACTTGTTATAATAGTTGAGATATCATCTTTATTTCATTTTAGCACATGCATGATTCTGGTTCCTGTTGCAATTTGGCCTCAAATTGATTTCCAAATTCTGTTTAGTTTGTTAATATGCTTTGTAAAGGCCCTTTTGAAACATTCAACCAAAACATTATTTGATATGTCTGACATTATATTGTGCTGTCGATGTGAATTGAGTTTCTGAAGCATTTACTTGAGTAGTTCACAGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCATGGGCAATTAGTACGCGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTAGCTCCCATACAGGTATTGATATTAAATTATAATTTTTATTTTGTTGAGTATCACTATCAGTTATTTCTATAGCTTAGAAGCCGTGTGAAATTGCCCTAAAAAAACAGCACTGCGAGATAAAAATCTCATGCACAGTTTTGAATGAGAGAAATGTGAGGAATCCTCTTTTCGATTTTGACTCTTCCACTCGACTAGTTGCTTTTCTTTTCATTACTTTGAAAGTAGCTGCTTCAGTCACTTGAATTTTTCGGTTTCGTCCGGAAATTTATATAGAATCCTTGAAAAAGACCCCATATATTTCTAATGGCAATTACCTGAGTTTGACTTGACGTAAAAGTGACTAGAGATTAGGTGCATAACATAGTTATCTTATGTCCAAACTCTTTGAAAACTTGAGAATAGTAAGAAGAATTTCTGATAATGCTGGCATGTTTCTGTTTTTTGAGCCCACCATTCATAGTATTCGTTTTTAACAAATATCTTTAAATATGCCTGACTTACAAGTTACATCTAGGTTGTAATCATACCCATCTGGAAGAAGGATGGTGATAAAGCAGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGGTAGTCTTCAAACTGCAGGGATAAAGGTTAAACTTGATGAGTCAGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGTTGGTGCTTATTTTTGATGATTAAGATCAATCTTTGACAATTCTCTTCTCCTCAGTTTAGGAAAATATTGTCTAAAAGATAAATACTAATGTTAAAAATTTCAGCATATGTTAACTGGTTAGTTGCCAATAGATGTTCATATTCTCTCTTTCATGATTTCTGTATCTTTTATGATTACTTTCACATCTTGGAATCTAGTTTATGAAAAGAATACTCGTCTGTAGTGTACCTGTAGGTTGGTTGGTTGGCCGGCATTTTTTTTTCAGGATGTATGCATATTGGTTATTGATAGTTTTGAATGTTCCAATTCCCTTTTTAGCTCCATGATTTTCACCTAGATTTTTCCTTTCACTCTCTCCCTCTCTCCCTCTCCCCCTCTCTCCCTCTCCCTTGTTGCTATTAACTTGAAATCAAATGTCTATAACTAGACTTGGATTTGTAGGGCGTTCCTTTGAGGATTGAGATTGGTCCTAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGACATTCCTGGAAAGCAAGGGAAAGTTTTTGGGATATCCATGGAACCTTCAATTTTAGAAGCGTATGTAAAAGACACGTTGGATAAGATCCAGTCATCCCTTCTTGAACAAGCAAAAACTTTTCGTGACAGGTGCTTGCTTATTTTCTTATGTTGGGATGTTTCTTGAAAAGTATGTAAATCTATGTTTAGCTTTAGTTACATTACGTTTATTCTTTGAAATTCAAGCAATTGAATGTTGAATAGCATGCTTCTTGCAAATTGTAGTACAATGTTACATACTAAGTAGTTAAATGTAAATACATTCTGTTGAATTGTGAGCTTCAAGAAAATTGTTCCCATCTACTCACCCTTATTTTCTTCCTGTCCTCGCCCCTTGCTTCCCCATGATTCTTCTTTCACCAGATTATGTATGAAAAAATTCTTTAATTGTTTCATTATATTTGAAAGGAAAGAAGGGCTGGGGAATGAATGGTGTGCTTCACAGTTCTCATTTTAATAGTTTTCGTGGTAAATTATATTCTTGATCAAAATTTGAAGTTGTAAATGGTGGCTTAATGCATTTGCATTCTCTGCATTTTTCCATCTAAATGAAATAAAAATCAAGGTTAATCTGACAGGACGATTAATTTTGTATAATAATGGATAAGCTCCAAAGCTATCACAGGATTGAAATGTTTTTAATATACTCTTGATGATATTTTATCTTAGTATATGAAAAACCTGCATTTCACGCCCCTTTCATAAGATATACCGTATTTTGTTAACAACCTCCTTTTTGCAGTAACATTGTGGATGTGACTTCGTATAATGAACTTAAAGAGGCAATCTCCCAAGGGAAATGGGCTAGGGGTCCTTGGTCAGCTAGGTAGGCTTATTTTCATTTGTTGGGATTTGTAGATAAAATTTTATTTGTATGTTTGTGTATAATAGCTTGCTTCTGTATGCTCTTGATCATTTTTGCAGTGACAAAGATGAATTGAGAGTTAAAGAAGAAACAGGAGCGACAATTAGATGCTTTCCTTTTGAACAGCCACAAGGGCCTAAAACATGCTTGATGACTGGTAACGCTGCTGAGGAAGTTGCCATTTTTGCGAAGTCTTACTGAGATATGATTTCAAGGCATGACTTAAAACCTCCAAGCCTTATTTATTTGGACCTTTAATCACATTCGTGCACTATCTTAAAGGTCTTGTTAAATTTTCCTCGAAAGGGAGCCTCCTATATATCAACTCTTCTAAGGCAAAGGCTCTGGCTAGCTGCTTGGCATTGGCATTTACCATCTGGAGTTTGACGTGTTTTTGTTCTCTGCCTGCCTAATTATTTTGTTTGGTGGCTCAATCCTTCATTTTTGGAGCACTGTTTAAGCGATGGATTTATGTATGAATTAAATAGATTGACAATCTCGTTAAAGCATAAGTAATTTTGTCTTCGTTTCATGTTTCATACACATGGGCTTGCTGTTTCGATTATACAATGTTGGTCAAAATGTACTGGTATTTTGTCTAATATTTAACCCCGTGGTGATTTTGTGATTTCTTCTGTCCATGGAAACAATTTTAGCATTAGAATACAAAAGTTCTTTGTTTGTATTCTCACTTGAATTTGGCGCAAATGTAACTTCTAGAATGAATTTTTGGGTTGAAATGATTCTGGCTCTCTTGAATGGACACAGGAACAGACAAGACAAGAACATGAATTAATTAGAAACTTCATATTTCGTACATGGTCATTTACGATAATGATACAGGTTCAAACTTTAAATTATGCAATGAACTTCAAGTTTTTTTTTTTTTTTTTTTTT

mRNA sequence

ATGGTAGTGTCTCTGAGACTACCTTCGCTCTCCTCTTTGTTTTCCCAAGCCTCACCTCGCTCCTCAAGCTTCACCTTCTCCAGGCGAGCTCTGGGGATTTCATGGCGCCAGCAACGCCCGTCCCGGTTGCCGCTTCTTGCTTCTGGATTCTCCGCGCGAAGTTCGTCCACTGGGACCGAAATCCGTGTCGACAACTCGAAGAATCGAGTTCCGGAAGGAGCCATCACGCCTCGGTCTCAGGACTTCAATGCTTGGTATTTGGATGTCATTGCTAATGCTGAGCTTGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCTTATGGCTACGCAATTTGGGAAGCAATACAGGTGGTGGAAGCTATATTTTCTGAATATTTGAATGTTAAGTTCAAGGAGACCGGTCATCAGAATATGTACTTTCCACAGTTTATACCATACTCGTTCATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCGGAGTTAGCTCTTGTAACAATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTACGTAGACTCAGCAAAGCTATTGGCCCTTTTCCAGTTCGACCTACTAGTGAAACCATTGTGAATCACATGTTTACCCAGTGGATTCATAGTTACCGTGATCTTCCTTTGATGATTAATCAGTGGGCGAATGTTACTAGATGGGAGATGCGCACGAAACCGTTTGTAAGAACTCTTGAATTTCTCTGGCAGGAGGGTCATACTGCTCATGTCAGTCCAGAAGAGGCAGAAAAAGAGGCTATTCAGATGATTGACATATATACAAAATTTGCTTATGAGCAAGCGGCAATACCTGTTATTGCAGGTCGAAAATCAAAGGTGGAGACATTTGCTGGTGCAGACAAGACCTATACAATAGAGGCCATGATGGGGGATCGAAAAGCACTGCAGGCAGGAACCAGCCACAACCTTGGGCAAAACTTTTCTCGTGCTTTTGGAACACAGTTCACAGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCATGGGCAATTAGTACGCGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTAGCTCCCATACAGGTTGTAATCATACCCATCTGGAAGAAGGATGGTGATAAAGCAGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGGTAGTCTTCAAACTGCAGGGATAAAGGTTAAACTTGATGAGTCAGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGGCGTTCCTTTGAGGATTGAGATTGGTCCTAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGACATTCCTGGAAAGCAAGGGAAAGTTTTTGGGATATCCATGGAACCTTCAATTTTAGAAGCGTATGTAAAAGACACGTTGGATAAGATCCAGTCATCCCTTCTTGAACAAGCAAAAACTTTTCGTGACAGTAACATTGTGGATGTGACTTCGTATAATGAACTTAAAGAGGCAATCTCCCAAGGGAAATGGGCTAGGGGTCCTTGGTCAGCTAGTGACAAAGATGAATTGAGAGTTAAAGAAGAAACAGGAGCGACAATTAGATGCTTTCCTTTTGAACAGCCACAAGGGCCTAAAACATGCTTGATGACTGGTAACGCTGCTGAGGAAGTTGCCATTTTTGCGAAGTCTTACTGA

Coding sequence (CDS)

ATGGTAGTGTCTCTGAGACTACCTTCGCTCTCCTCTTTGTTTTCCCAAGCCTCACCTCGCTCCTCAAGCTTCACCTTCTCCAGGCGAGCTCTGGGGATTTCATGGCGCCAGCAACGCCCGTCCCGGTTGCCGCTTCTTGCTTCTGGATTCTCCGCGCGAAGTTCGTCCACTGGGACCGAAATCCGTGTCGACAACTCGAAGAATCGAGTTCCGGAAGGAGCCATCACGCCTCGGTCTCAGGACTTCAATGCTTGGTATTTGGATGTCATTGCTAATGCTGAGCTTGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCTTATGGCTACGCAATTTGGGAAGCAATACAGGTGGTGGAAGCTATATTTTCTGAATATTTGAATGTTAAGTTCAAGGAGACCGGTCATCAGAATATGTACTTTCCACAGTTTATACCATACTCGTTCATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCGGAGTTAGCTCTTGTAACAATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTACGTAGACTCAGCAAAGCTATTGGCCCTTTTCCAGTTCGACCTACTAGTGAAACCATTGTGAATCACATGTTTACCCAGTGGATTCATAGTTACCGTGATCTTCCTTTGATGATTAATCAGTGGGCGAATGTTACTAGATGGGAGATGCGCACGAAACCGTTTGTAAGAACTCTTGAATTTCTCTGGCAGGAGGGTCATACTGCTCATGTCAGTCCAGAAGAGGCAGAAAAAGAGGCTATTCAGATGATTGACATATATACAAAATTTGCTTATGAGCAAGCGGCAATACCTGTTATTGCAGGTCGAAAATCAAAGGTGGAGACATTTGCTGGTGCAGACAAGACCTATACAATAGAGGCCATGATGGGGGATCGAAAAGCACTGCAGGCAGGAACCAGCCACAACCTTGGGCAAAACTTTTCTCGTGCTTTTGGAACACAGTTCACAGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCATGGGCAATTAGTACGCGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTAGCTCCCATACAGGTTGTAATCATACCCATCTGGAAGAAGGATGGTGATAAAGCAGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGGTAGTCTTCAAACTGCAGGGATAAAGGTTAAACTTGATGAGTCAGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGGCGTTCCTTTGAGGATTGAGATTGGTCCTAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGACATTCCTGGAAAGCAAGGGAAAGTTTTTGGGATATCCATGGAACCTTCAATTTTAGAAGCGTATGTAAAAGACACGTTGGATAAGATCCAGTCATCCCTTCTTGAACAAGCAAAAACTTTTCGTGACAGTAACATTGTGGATGTGACTTCGTATAATGAACTTAAAGAGGCAATCTCCCAAGGGAAATGGGCTAGGGGTCCTTGGTCAGCTAGTGACAAAGATGAATTGAGAGTTAAAGAAGAAACAGGAGCGACAATTAGATGCTTTCCTTTTGAACAGCCACAAGGGCCTAAAACATGCTTGATGACTGGTAACGCTGCTGAGGAAGTTGCCATTTTTGCGAAGTCTTACTGA

Protein sequence

MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVVEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATIRCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY

Homology

[Close]

BLAST of Spg002527 vs. NCBI nr
Match: XP_038878181.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.6e-302
Identity = 525/571 (91.94%), Postives = 540/571 (94.57%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTE 60
           MVVSLRLP +SSL S AS RSS+FTFSRRALGISWRQQRP RLPLL+SGFSARSSSTGTE
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNFTFSRRALGISWRQQRPLRLPLLSSGFSARSSSTGTE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV
Sbjct: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120

Query: 121 VEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180
                  +LN KFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK
Sbjct: 121 -------FLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180

Query: 181 LVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240
           LV            VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RT
Sbjct: 181 LV------------VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRT 240

Query: 241 LEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300
           LEFLWQEGHTAH SPEEAEKEAIQMID+YTKF+YEQAAIPVIAGRKSKVETFAGADKTYT
Sbjct: 241 LEFLWQEGHTAHASPEEAEKEAIQMIDVYTKFSYEQAAIPVIAGRKSKVETFAGADKTYT 300

Query: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360
           IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH
Sbjct: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360

Query: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTP 420
           GDDGGLMLPPKLAPIQVVIIPIWKKDGDK EVLSAASSVK SLQTAGIKVKLDESEQRTP
Sbjct: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKVEVLSAASSVKESLQTAGIKVKLDESEQRTP 420

Query: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480
           GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPS+LEAYVK TL
Sbjct: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSVLEAYVKGTL 480

Query: 481 DKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540
           D+IQSSLL+ AKTFRDSNIVDV+SY+ELKEAIS+GKWARGPWSASD+DELRVKEETGATI
Sbjct: 481 DEIQSSLLQLAKTFRDSNIVDVSSYDELKEAISEGKWARGPWSASDQDELRVKEETGATI 540

Query: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           RCFPFEQPQGPKTCLMTGN AEEVAIFAKSY
Sbjct: 541 RCFPFEQPQGPKTCLMTGNPAEEVAIFAKSY 552

[Close]

BLAST of Spg002527 vs. NCBI nr
Match: XP_023541480.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.3e-300
Identity = 523/571 (91.59%), Postives = 538/571 (94.22%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTE 60
           MVVSLRLPSLSSL S ASP    FTFSRRALGISWRQQRP RLPLLA GFS RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISWRQQRPFRLPLLAPGFSVRSSSTGTE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDNSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQ- 120

Query: 121 VEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180
                 EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK
Sbjct: 121 ------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180

Query: 181 LVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240
           LV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRT
Sbjct: 181 LV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRT 240

Query: 241 LEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300
           LEFLWQEGHTAH SPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT
Sbjct: 241 LEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300

Query: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360
           IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH
Sbjct: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360

Query: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTP 420
           GDDGGLMLPP+LAPIQVVIIPIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD SEQRTP
Sbjct: 361 GDDGGLMLPPRLAPIQVVIIPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTP 420

Query: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480
           GWKFNFWEMKGVPLRIEIGPKDVA+QSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL
Sbjct: 421 GWKFNFWEMKGVPLRIEIGPKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480

Query: 481 DKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540
           D+IQSSLLEQAKTFRDSN+VDV+SYNELKEAISQGKWARGPWSASDKDEL+VKEETGATI
Sbjct: 481 DEIQSSLLEQAKTFRDSNVVDVSSYNELKEAISQGKWARGPWSASDKDELKVKEETGATI 540

Query: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY
Sbjct: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 548

[Close]

BLAST of Spg002527 vs. NCBI nr
Match: XP_022945172.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.7e-299
Identity = 521/571 (91.24%), Postives = 538/571 (94.22%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTE 60
           MVVSLRLPSLSSL S ASP    FTFSRRALGISWRQQRP RLPLLA G S RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISWRQQRPFRLPLLAPGLSVRSSSTGTE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDNSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQ- 120

Query: 121 VEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180
                 EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK
Sbjct: 121 ------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180

Query: 181 LVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240
           LV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRT
Sbjct: 181 LV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRT 240

Query: 241 LEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300
           LEFLWQEGHTAH SPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT
Sbjct: 241 LEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300

Query: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360
           IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH
Sbjct: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360

Query: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTP 420
           GDDGGLMLPP+LAPIQVVI+PIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD+SEQRTP
Sbjct: 361 GDDGGLMLPPRLAPIQVVIVPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDDSEQRTP 420

Query: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480
           GWKFNFWEMKGVPLRIEIGPKDVA+QSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL
Sbjct: 421 GWKFNFWEMKGVPLRIEIGPKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480

Query: 481 DKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540
           D+IQSSLLEQAKTFRDSN+VDV+SYNELKEAISQGKWARGPWSASDKDEL+VKEETGATI
Sbjct: 481 DEIQSSLLEQAKTFRDSNVVDVSSYNELKEAISQGKWARGPWSASDKDELKVKEETGATI 540

Query: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY
Sbjct: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 548

[Close]

BLAST of Spg002527 vs. NCBI nr
Match: XP_022968367.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 1026.2 bits (2652), Expect = 1.1e-295
Identity = 518/571 (90.72%), Postives = 534/571 (93.52%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTE 60
           MVVSLRLPSLSSL S ASP    FTFSRRALGIS RQQRP RLPLLA  FS RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISLRQQRPFRLPLLAPRFSVRSSSTGTE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDNSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQ- 120

Query: 121 VEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180
                 EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK
Sbjct: 121 ------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180

Query: 181 LVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240
           LV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRT
Sbjct: 181 LV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRT 240

Query: 241 LEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300
           LEFLWQEGHTAH SPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSK+ETFAGADKTYT
Sbjct: 241 LEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKMETFAGADKTYT 300

Query: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360
           IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH
Sbjct: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360

Query: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTP 420
           GDDGGLMLPP+LAPIQVVIIPIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD SEQRTP
Sbjct: 361 GDDGGLMLPPRLAPIQVVIIPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTP 420

Query: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480
           GWKFNFWEMKGVPLRIEIGPKDVA+QSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL
Sbjct: 421 GWKFNFWEMKGVPLRIEIGPKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480

Query: 481 DKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540
           D+IQSSLLEQAKTFRDSN+VDV+SYNELKEAISQGKWARGPWSASDKDEL+VKEETGATI
Sbjct: 481 DEIQSSLLEQAKTFRDSNVVDVSSYNELKEAISQGKWARGPWSASDKDELKVKEETGATI 540

Query: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           RCFPFEQ  GPKTCLMTGNAAEEVAIFAKSY
Sbjct: 541 RCFPFEQTPGPKTCLMTGNAAEEVAIFAKSY 548

[Close]

BLAST of Spg002527 vs. NCBI nr
Match: XP_004149956.3 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >XP_031738676.1 proline--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN57582.2 hypothetical protein Csa_011595 [Cucumis sativus])

HSP 1 Score: 1014.2 bits (2621), Expect = 4.4e-292
Identity = 510/574 (88.85%), Postives = 531/574 (92.51%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQR---PSRLPLLASGFSARSSST 60
           MVVSLRLP +SSL S AS RSS+  FSRRALGISWR QR   P R PLL+S FS RSSST
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNLAFSRRALGISWRHQRQRPPLRFPLLSSPFSPRSSST 60

Query: 61  GTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120
            TEI +D+SKNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA
Sbjct: 61  NTEIHIDSSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120

Query: 121 IQVVEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKEL 180
           IQ       EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKEL
Sbjct: 121 IQ-------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKEL 180

Query: 181 EEKLVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF 240
           EEKLV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPF
Sbjct: 181 EEKLV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPF 240

Query: 241 VRTLEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADK 300
           VRTLEFLWQEGHTAH SPEEAEKEAIQMIDIYTKF+YEQAAIPVIAGRKSKVETFAGADK
Sbjct: 241 VRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADK 300

Query: 301 TYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGII 360
           TYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGII
Sbjct: 301 TYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGII 360

Query: 361 MTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQ 420
           MTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVL+AASSVK  LQTAG+KVKLDES+Q
Sbjct: 361 MTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQ 420

Query: 421 RTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVK 480
           +T GWKFNFWEMKGVPLRIEIGPKDVAS+SVVISRRDIPGKQGKVFGISMEPS+LEAYVK
Sbjct: 421 KTAGWKFNFWEMKGVPLRIEIGPKDVASKSVVISRRDIPGKQGKVFGISMEPSVLEAYVK 480

Query: 481 DTLDKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETG 540
           + LD+IQSSLL+QA+TFRDSNIVDV+SYNELKEAIS+GKWARGPWSASD+DELRVKEETG
Sbjct: 481 ERLDEIQSSLLQQARTFRDSNIVDVSSYNELKEAISEGKWARGPWSASDQDELRVKEETG 540

Query: 541 ATIRCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           ATIRCFPFEQPQGPKTCLMTGN AEEVAIFAKSY
Sbjct: 541 ATIRCFPFEQPQGPKTCLMTGNPAEEVAIFAKSY 555

[Close]

BLAST of Spg002527 vs. ExPASy Swiss-Prot
Match: Q9FYR6 (Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA6 PE=2 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 7.1e-253
Identity = 442/569 (77.68%), Postives = 486/569 (85.41%), Query Frame = 0


Query: 4   SLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTEIRV 63
           SLRLPSL+SL   A+ R  +    RR + +   + RP       SGF+   S T +    
Sbjct: 5   SLRLPSLTSLLFPATTRYPATL--RRTVCL---RNRP------LSGFATAPSGTASPETK 64

Query: 64  DNSKNRV-PEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVVE 123
            +  +R+  + A+TPRSQDFNAWYLDVIA+AELADYGPVRGTMVIRPYGYAIWEAIQ   
Sbjct: 65  SSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQ--- 124

Query: 124 AIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLV 183
               +YLNVKFKETGH NMYFPQFIPYSFIEKEASHVEGFSPELALVT+GGGKELEEKLV
Sbjct: 125 ----DYLNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLV 184

Query: 184 VRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLE 243
                       VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLE
Sbjct: 185 ------------VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLE 244

Query: 244 FLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIE 303
           FLWQEGHTAH +PEEAEKEA QMI+IYT+FA+EQ AIPVI GRKSK+ETFAGAD TYTIE
Sbjct: 245 FLWQEGHTAHATPEEAEKEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIE 304

Query: 304 AMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGD 363
           AMMGDRKALQAGTSHNLGQNFSRAFGTQF DENG+RQHVWQTSWA+STRFVGGIIMTHGD
Sbjct: 305 AMMGDRKALQAGTSHNLGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGD 364

Query: 364 DGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTPGW 423
           D GLMLPPK+APIQVVI+PIWKKD +K  VLSAASSVK +LQTAG++VKLD+++QRTPGW
Sbjct: 365 DTGLMLPPKIAPIQVVIVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGW 424

Query: 424 KFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDK 483
           KFNFWEMKG+PLRIEIGP+DV+S SVV+SRRD+PGK GKVFGISMEPS L AYVK+ LD+
Sbjct: 425 KFNFWEMKGIPLRIEIGPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDE 484

Query: 484 IQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATIRC 543
           IQ+SLLE+A +FRDSNIVDV SY ELK+AIS GKWARGPWSASD DE RVKEETGATIRC
Sbjct: 485 IQTSLLEKALSFRDSNIVDVNSYAELKDAISSGKWARGPWSASDADEQRVKEETGATIRC 543

Query: 544 FPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           FPFEQ QG KTCLMTGN AEEVAIFAKSY
Sbjct: 545 FPFEQTQGTKTCLMTGNPAEEVAIFAKSY 543

[Close]

BLAST of Spg002527 vs. ExPASy Swiss-Prot
Match: Q1CXV1 (Proline--tRNA ligase OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=proS PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 7.5e-146
Identity = 257/499 (51.50%), Postives = 349/499 (69.94%), Query Frame = 0


Query: 75  ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVVEAIFSEYLNVKFK 134
           +TPR + F+ WY+D++  A+LADY  V+G MVIRP GYA+WE +Q V       L+  FK
Sbjct: 11  LTPREKGFSEWYVDLVQKAKLADYSDVKGCMVIRPNGYALWENMQRV-------LDKMFK 70

Query: 135 ETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRRLSKAIGPFP 194
           + GH+N YFP  IP S+++KEA HVEGF+P+LA+VT  GG +LEE            P+ 
Sbjct: 71  DLGHKNAYFPLLIPESYLKKEAEHVEGFNPQLAVVTHAGGSKLEE------------PYV 130

Query: 195 VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHVS 254
           +RPTSETI+N  F +WI SYRDLPL++NQWANV RWEMRT+ F+RT EFLWQEGHT H +
Sbjct: 131 IRPTSETIINRSFAKWIQSYRDLPLLLNQWANVMRWEMRTRLFLRTTEFLWQEGHTCHET 190

Query: 255 PEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAG 314
            E+AEKE  QM+++Y  FA +  A+PV+ G+KS+ E FAGA +TY+IEAMM D+KALQAG
Sbjct: 191 EEDAEKETRQMLEVYRTFAEDYMAMPVMTGQKSESERFAGALRTYSIEAMMQDKKALQAG 250

Query: 315 TSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAP 374
           TSHNLGQNF++AF TQF   +G+  HVWQTSW +STR +GG+IMTH DD GL++PPKLA 
Sbjct: 251 TSHNLGQNFAKAFDTQFQGRDGKMHHVWQTSWGVSTRLIGGLIMTHSDDAGLIVPPKLAA 310

Query: 375 IQVVIIPIWKK--DGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTPGWKFNFWEMKGV 434
             VVIIPI+ K  D +KA+VL   +++   L+ AG+ V LD+ + ++PG+K+N  E+ G 
Sbjct: 311 THVVIIPIFGKASDAEKAQVLEKTNALAADLRKAGLGVVLDDDDTKSPGFKYNEHELIGT 370

Query: 435 PLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDKIQSSLLEQAK 494
            LRIE+GPKD+A  S V+ RRD+  K+     +S++ ++ +A  +  LD +Q  L  +AK
Sbjct: 371 CLRIELGPKDLAKNSCVMVRRDVRQKE----FVSLDEAVSKA--QAMLDAMQKDLFTKAK 430

Query: 495 TFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATIRCFPFEQPQGPK 554
           +FRDS+  +V SY ELKE   QG +    W+   K E R+KEETG T RC PF+  Q P 
Sbjct: 431 SFRDSHTFEVNSYEELKEKADQG-FLLAHWNLDPKVEARIKEETGLTTRCRPFDLKQEPG 483

Query: 555 TCLMTGNAAEEVAIFAKSY 572
            C++TG  +    +F+K+Y
Sbjct: 491 KCVVTGEPSPGRIVFSKAY 483

[Close]

BLAST of Spg002527 vs. ExPASy Swiss-Prot
Match: A9W9Q2 (Proline--tRNA ligase OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) OX=324602 GN=proS PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.7e-145
Identity = 252/504 (50.00%), Postives = 353/504 (70.04%), Query Frame = 0


Query: 70  VPEGAITPRSQDFNAWYLDVIANAELADYGP-VRGTMVIRPYGYAIWEAIQVVEAIFSEY 129
           +P+  IT RS D+N WYLD++  A+LA+    VRG +V++ +G+AIWE +Q         
Sbjct: 1   MPKEVITQRSVDYNQWYLDIVREADLAEVAEVVRGCIVVKAHGWAIWELMQ-------RA 60

Query: 130 LNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRRLSK 189
           L+ + K TGH N+ FP  IP SFI KEA HVEGF+PE+A VT  GG+EL E         
Sbjct: 61  LDDRIKATGHANVQFPLLIPKSFIMKEAEHVEGFAPEVAEVTRAGGEELAE--------- 120

Query: 190 AIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEG 249
              P+ +RPTSETI+ + +++WI SYRDLPL+ NQWANV RWEMRT+PF+RT EF WQEG
Sbjct: 121 ---PYIIRPTSETIIGYFYSKWIRSYRDLPLLYNQWANVMRWEMRTRPFLRTAEFWWQEG 180

Query: 250 HTAHVSPEEAEKEAIQMI-DIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGD 309
           HTAH +  EAE+E ++++ D+Y  F  ++ A+PVI G K++ E F GA ++Y IEAMM D
Sbjct: 181 HTAHATEAEAEEETLRILHDVYADFVEKEMAVPVIRGLKTEKEKFPGALRSYCIEAMMQD 240

Query: 310 RKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLM 369
            +ALQAGTSHNLGQNF+RAF   FTD+N   Q+ W TSW +STR +G +IMTH DD GL+
Sbjct: 241 GRALQAGTSHNLGQNFARAFDITFTDQNNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLV 300

Query: 370 LPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTPGWKFNFW 429
           LPP+LAPIQVV++PI+K D +++ V++A   +  + +   ++ K+D+ +  +PG+KFN W
Sbjct: 301 LPPRLAPIQVVVVPIYKNDEERSLVMAAVEQMTAAWK-GRLRFKVDDRDNYSPGYKFNEW 360

Query: 430 EMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDKIQSSL 489
           E+KGVP+RIE+GPKDVA ++V ++RRDIPGK GK F   +  + L   ++  L+++Q++L
Sbjct: 361 ELKGVPVRIEVGPKDVAKETVALARRDIPGKAGKSF---VPQAGLTERIEALLNEMQTAL 420

Query: 490 LEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATIRCFPFEQ 549
            ++A  FR+++  DVTSY+ELKE I +G +AR  W+    DE R++EET ATIRC P EQ
Sbjct: 421 FQRALAFREAHTADVTSYDELKEQIERG-FARAYWAGDTADEKRIQEETRATIRCIPLEQ 480

Query: 550 PQGPKTCLMTGNAAEEVAIFAKSY 572
           P     C+ TG   +   IFA++Y
Sbjct: 481 PGSVGRCVYTGRETDRQVIFARAY 480

[Close]

BLAST of Spg002527 vs. ExPASy Swiss-Prot
Match: B9LC55 (Proline--tRNA ligase OS=Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) OX=480224 GN=proS PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.7e-145
Identity = 252/504 (50.00%), Postives = 353/504 (70.04%), Query Frame = 0


Query: 70  VPEGAITPRSQDFNAWYLDVIANAELADYGP-VRGTMVIRPYGYAIWEAIQVVEAIFSEY 129
           +P+  IT RS D+N WYLD++  A+LA+    VRG +V++ +G+AIWE +Q         
Sbjct: 1   MPKEVITQRSVDYNQWYLDIVREADLAEVAEVVRGCIVVKAHGWAIWELMQ-------RA 60

Query: 130 LNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRRLSK 189
           L+ + K TGH N+ FP  IP SFI KEA HVEGF+PE+A VT  GG+EL E         
Sbjct: 61  LDDRIKATGHANVQFPLLIPKSFIMKEAEHVEGFAPEVAEVTRAGGEELAE--------- 120

Query: 190 AIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEG 249
              P+ +RPTSETI+ + +++WI SYRDLPL+ NQWANV RWEMRT+PF+RT EF WQEG
Sbjct: 121 ---PYIIRPTSETIIGYFYSKWIRSYRDLPLLYNQWANVMRWEMRTRPFLRTAEFWWQEG 180

Query: 250 HTAHVSPEEAEKEAIQMI-DIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGD 309
           HTAH +  EAE+E ++++ D+Y  F  ++ A+PVI G K++ E F GA ++Y IEAMM D
Sbjct: 181 HTAHATEAEAEEETLRILHDVYADFVEKEMAVPVIRGLKTEKEKFPGALRSYCIEAMMQD 240

Query: 310 RKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLM 369
            +ALQAGTSHNLGQNF+RAF   FTD+N   Q+ W TSW +STR +G +IMTH DD GL+
Sbjct: 241 GRALQAGTSHNLGQNFARAFDITFTDQNNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLV 300

Query: 370 LPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTPGWKFNFW 429
           LPP+LAPIQVV++PI+K D +++ V++A   +  + +   ++ K+D+ +  +PG+KFN W
Sbjct: 301 LPPRLAPIQVVVVPIYKNDEERSLVMAAVEQMTAAWK-GRLRFKVDDRDNYSPGYKFNEW 360

Query: 430 EMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDKIQSSL 489
           E+KGVP+RIE+GPKDVA ++V ++RRDIPGK GK F   +  + L   ++  L+++Q++L
Sbjct: 361 ELKGVPVRIEVGPKDVAKETVALARRDIPGKAGKSF---VPQAGLTERIEALLNEMQTAL 420

Query: 490 LEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATIRCFPFEQ 549
            ++A  FR+++  DVTSY+ELKE I +G +AR  W+    DE R++EET ATIRC P EQ
Sbjct: 421 FQRALAFREAHTADVTSYDELKEQIERG-FARAYWAGDTADEKRIQEETRATIRCIPLEQ 480

Query: 550 PQGPKTCLMTGNAAEEVAIFAKSY 572
           P     C+ TG   +   IFA++Y
Sbjct: 481 PGSVGRCVYTGRETDRQVIFARAY 480

[Close]

BLAST of Spg002527 vs. ExPASy Swiss-Prot
Match: A7NKX0 (Proline--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=383372 GN=proS PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 4.9e-145
Identity = 249/504 (49.40%), Postives = 350/504 (69.44%), Query Frame = 0


Query: 70  VPEGAITPRSQDFNAWYLDVIANAELADYGP-VRGTMVIRPYGYAIWEAIQVVEAIFSEY 129
           +P+  ITPR+QD++ WYLD++  A+LADY   V+G +V +P GYA+WEAIQ         
Sbjct: 1   MPKEGITPRAQDYSQWYLDIVQQADLADYAEVVKGCIVFKPTGYALWEAIQ-------RG 60

Query: 130 LNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRRLSK 189
           L+ + K TGH N YFP  IP SF+ KEA HVEGF+PE+A VT  GG++L E         
Sbjct: 61  LDDRIKATGHVNAYFPLLIPKSFLMKEAEHVEGFAPEVAEVTRAGGEDLAE--------- 120

Query: 190 AIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEG 249
              P+ +RPTSETI+ + +++W+ SYRDLPL+INQWANV RWEMRT+PF+RT EFLWQEG
Sbjct: 121 ---PYVIRPTSETIIGYFYSKWVRSYRDLPLLINQWANVMRWEMRTRPFLRTTEFLWQEG 180

Query: 250 HTAHVSPEEAEKEAIQMI-DIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGD 309
           HT H + E+AE+E + ++ ++Y  F  +  AIPVI G KS+ E F GA ++Y IEAMM D
Sbjct: 181 HTVHATEEDAERETLLILHEVYADFVEKDMAIPVIRGLKSEKEKFPGALRSYCIEAMMQD 240

Query: 310 RKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLM 369
            +ALQAGTSHNLGQNF+RAF   +TD++   Q+ W TSW +STR +G +IMTH DD GL+
Sbjct: 241 GRALQAGTSHNLGQNFARAFDITYTDQHNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLV 300

Query: 370 LPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTPGWKFNFW 429
           +PP+LAP QVV++PI++ D +++ V+ A   +    +   ++ K+D+ +  TPG+KFN W
Sbjct: 301 IPPRLAPTQVVVVPIYRNDAERSVVMEAVQRMTAEWKGL-LRFKVDDRDNLTPGFKFNEW 360

Query: 430 EMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDKIQSSL 489
           E+KGVP+R+EIGPKD+   SV I+RRD PG++GK F   +    L A +   L++IQ +L
Sbjct: 361 ELKGVPIRVEIGPKDIEKGSVAIARRDQPGREGKSF---VPQEGLTARLAALLEEIQQAL 420

Query: 490 LEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATIRCFPFEQ 549
             +A  FR+++  DVT+Y ELK+ + +G +AR  W+ + +DE R++EET ATIRC P +Q
Sbjct: 421 YRRALAFRETHTADVTTYEELKQQVERG-FARCYWAGTMEDEKRIQEETRATIRCIPLDQ 480

Query: 550 PQGPKTCLMTGNAAEEVAIFAKSY 572
           PQ    C+ TG    +  IFA++Y
Sbjct: 481 PQQAGRCIYTGKETTQQVIFARAY 480

[Close]

BLAST of Spg002527 vs. ExPASy TrEMBL
Match: A0A6J1G067 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111449493 PE=3 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 8.0e-300
Identity = 521/571 (91.24%), Postives = 538/571 (94.22%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTE 60
           MVVSLRLPSLSSL S ASP    FTFSRRALGISWRQQRP RLPLLA G S RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISWRQQRPFRLPLLAPGLSVRSSSTGTE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDNSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQ- 120

Query: 121 VEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180
                 EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK
Sbjct: 121 ------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180

Query: 181 LVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240
           LV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRT
Sbjct: 181 LV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRT 240

Query: 241 LEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300
           LEFLWQEGHTAH SPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT
Sbjct: 241 LEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300

Query: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360
           IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH
Sbjct: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360

Query: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTP 420
           GDDGGLMLPP+LAPIQVVI+PIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD+SEQRTP
Sbjct: 361 GDDGGLMLPPRLAPIQVVIVPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDDSEQRTP 420

Query: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480
           GWKFNFWEMKGVPLRIEIGPKDVA+QSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL
Sbjct: 421 GWKFNFWEMKGVPLRIEIGPKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480

Query: 481 DKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540
           D+IQSSLLEQAKTFRDSN+VDV+SYNELKEAISQGKWARGPWSASDKDEL+VKEETGATI
Sbjct: 481 DEIQSSLLEQAKTFRDSNVVDVSSYNELKEAISQGKWARGPWSASDKDELKVKEETGATI 540

Query: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY
Sbjct: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 548

[Close]

BLAST of Spg002527 vs. ExPASy TrEMBL
Match: A0A6J1HTC6 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111467631 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.4e-296
Identity = 518/571 (90.72%), Postives = 534/571 (93.52%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTE 60
           MVVSLRLPSLSSL S ASP    FTFSRRALGIS RQQRP RLPLLA  FS RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISLRQQRPFRLPLLAPRFSVRSSSTGTE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDNSKNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQ- 120

Query: 121 VEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180
                 EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK
Sbjct: 121 ------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180

Query: 181 LVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240
           LV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRT
Sbjct: 181 LV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRT 240

Query: 241 LEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300
           LEFLWQEGHTAH SPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSK+ETFAGADKTYT
Sbjct: 241 LEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKMETFAGADKTYT 300

Query: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360
           IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH
Sbjct: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360

Query: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTP 420
           GDDGGLMLPP+LAPIQVVIIPIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD SEQRTP
Sbjct: 361 GDDGGLMLPPRLAPIQVVIIPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTP 420

Query: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480
           GWKFNFWEMKGVPLRIEIGPKDVA+QSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL
Sbjct: 421 GWKFNFWEMKGVPLRIEIGPKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480

Query: 481 DKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540
           D+IQSSLLEQAKTFRDSN+VDV+SYNELKEAISQGKWARGPWSASDKDEL+VKEETGATI
Sbjct: 481 DEIQSSLLEQAKTFRDSNVVDVSSYNELKEAISQGKWARGPWSASDKDELKVKEETGATI 540

Query: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           RCFPFEQ  GPKTCLMTGNAAEEVAIFAKSY
Sbjct: 541 RCFPFEQTPGPKTCLMTGNAAEEVAIFAKSY 548

[Close]

BLAST of Spg002527 vs. ExPASy TrEMBL
Match: A0A6J1C8A3 (Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.7e-289
Identity = 507/571 (88.79%), Postives = 527/571 (92.29%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTE 60
           MVVSLRLPSLS+LFS AS  ++S TF RRA      QQR   LP LA+G+SA+SSST TE
Sbjct: 1   MVVSLRLPSLSALFSPASTTTTSTTFFRRAPVTLRPQQRLFHLPRLAAGYSAQSSSTETE 60

Query: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           IR DNSKNRVPEGAITPRS+DFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IRADNSKNRVPEGAITPRSKDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQA 120

Query: 121 VEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180
                  YLNVKFKETGH NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK
Sbjct: 121 -------YLNVKFKETGHDNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEK 180

Query: 181 LVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240
           LV            VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT
Sbjct: 181 LV------------VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRT 240

Query: 241 LEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYT 300
           LEFLWQEGHTAH +PEEAEKEAIQMIDIYTKFAYEQAA+PVI GRKSKVETFAGADKTYT
Sbjct: 241 LEFLWQEGHTAHANPEEAEKEAIQMIDIYTKFAYEQAAMPVIPGRKSKVETFAGADKTYT 300

Query: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTH 360
           IEAMMGDRKALQAGTSHNLGQNFSRAFGTQF DENGQRQHVWQTSWAISTRFVGGIIMTH
Sbjct: 301 IEAMMGDRKALQAGTSHNLGQNFSRAFGTQFADENGQRQHVWQTSWAISTRFVGGIIMTH 360

Query: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTP 420
           GDDGGLMLPPKLAPIQVVIIPIWKKD D+AEVLSA+SSVK SLQTAGIKVKLD+S+QRTP
Sbjct: 361 GDDGGLMLPPKLAPIQVVIIPIWKKDDDRAEVLSASSSVKESLQTAGIKVKLDDSDQRTP 420

Query: 421 GWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480
           GWKFNFWEMKGVPLRIEIGP+DV+S+SVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL
Sbjct: 421 GWKFNFWEMKGVPLRIEIGPRDVSSRSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTL 480

Query: 481 DKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540
           D+IQSSLL+QAKTFRDSNIVDV+SYNELKEAISQGKWARGPWSASDKDELRVKEETGATI
Sbjct: 481 DEIQSSLLQQAKTFRDSNIVDVSSYNELKEAISQGKWARGPWSASDKDELRVKEETGATI 540

Query: 541 RCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           RCFPFEQPQG KTCLMTGNAAE+VAIFAKSY
Sbjct: 541 RCFPFEQPQGRKTCLMTGNAAEDVAIFAKSY 552

[Close]

BLAST of Spg002527 vs. ExPASy TrEMBL
Match: A0A1S3C2J3 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496137 PE=3 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 4.7e-276
Identity = 475/522 (91.00%), Postives = 493/522 (94.44%), Query Frame = 0


Query: 50  FSARSSSTGTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRP 109
           FS RSSST TEIR ++SKNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRP
Sbjct: 42  FSPRSSSTNTEIRTESSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRP 101

Query: 110 YGYAIWEAIQVVEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALV 169
           YGYAIWEAIQ       EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALV
Sbjct: 102 YGYAIWEAIQ-------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALV 161

Query: 170 TIGGGKELEEKLVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTR 229
           TIGGGKELEEKLV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTR
Sbjct: 162 TIGGGKELEEKLV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTR 221

Query: 230 WEMRTKPFVRTLEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKV 289
           WEMRTKPFVRTLEFLWQEGHTAH SPEEAEKEAIQMIDIYTKF+YEQAAIPVIAGRKSKV
Sbjct: 222 WEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKV 281

Query: 290 ETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAIS 349
           ETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAIS
Sbjct: 282 ETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAIS 341

Query: 350 TRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIK 409
           TRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVL+AASSVK  LQTAG+K
Sbjct: 342 TRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLNAASSVKEFLQTAGLK 401

Query: 410 VKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEP 469
           VKLDES+Q+T GWKFNFWEMKGVPLRIEIGPKDVAS+SVVISRRDIPGKQGKVFGISMEP
Sbjct: 402 VKLDESDQKTAGWKFNFWEMKGVPLRIEIGPKDVASKSVVISRRDIPGKQGKVFGISMEP 461

Query: 470 SILEAYVKDTLDKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDE 529
           S+LEAYVK+TLD+IQSSLL+QA+TFRDSNIVDV+SYNELKEAIS+GKWARGPWSASD+DE
Sbjct: 462 SVLEAYVKETLDEIQSSLLQQARTFRDSNIVDVSSYNELKEAISEGKWARGPWSASDQDE 521

Query: 530 LRVKEETGATIRCFPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           LRVKEETGATIRCFPFEQPQGPKTCLMTGN AEEVAIFAKSY
Sbjct: 522 LRVKEETGATIRCFPFEQPQGPKTCLMTGNPAEEVAIFAKSY 544

[Close]

BLAST of Spg002527 vs. ExPASy TrEMBL
Match: A0A0A0L6I6 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G213510 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.6e-271
Identity = 478/542 (88.19%), Postives = 499/542 (92.07%), Query Frame = 0


Query: 1   MVVSLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQR---PSRLPLLASGFSARSSST 60
           MVVSLRLP +SSL S AS RSS+  FSRRALGISWR QR   P R PLL+S FS RSSST
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNLAFSRRALGISWRHQRQRPPLRFPLLSSPFSPRSSST 60

Query: 61  GTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120
            TEI +D+SKNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA
Sbjct: 61  NTEIHIDSSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120

Query: 121 IQVVEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKEL 180
           IQ       EYLNVKFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKEL
Sbjct: 121 IQ-------EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKEL 180

Query: 181 EEKLVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF 240
           EEKLV            VRPTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPF
Sbjct: 181 EEKLV------------VRPTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPF 240

Query: 241 VRTLEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADK 300
           VRTLEFLWQEGHTAH SPEEAEKEAIQMIDIYTKF+YEQAAIPVIAGRKSKVETFAGADK
Sbjct: 241 VRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADK 300

Query: 301 TYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGII 360
           TYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGII
Sbjct: 301 TYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGII 360

Query: 361 MTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQ 420
           MTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVL+AASSVK  LQTAG+KVKLDES+Q
Sbjct: 361 MTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQ 420

Query: 421 RTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVK 480
           +T GWKFNFWEMKGVPLRIEIGPKDVAS+SVVISRRDIPGKQGKVFGISMEPS+LEAYVK
Sbjct: 421 KTAGWKFNFWEMKGVPLRIEIGPKDVASKSVVISRRDIPGKQGKVFGISMEPSVLEAYVK 480

Query: 481 DTLDKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETG 540
           + LD+IQSSLL+QA+TFRDSNIVDV+SYNELKEAIS+GKWARGPWSASD+DELRVKEET 
Sbjct: 481 ERLDEIQSSLLQQARTFRDSNIVDVSSYNELKEAISEGKWARGPWSASDQDELRVKEETR 523

[Close]

BLAST of Spg002527 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 874.4 bits (2258), Expect = 5.0e-254
Identity = 442/569 (77.68%), Postives = 486/569 (85.41%), Query Frame = 0


Query: 4   SLRLPSLSSLFSQASPRSSSFTFSRRALGISWRQQRPSRLPLLASGFSARSSSTGTEIRV 63
           SLRLPSL+SL   A+ R  +    RR + +   + RP       SGF+   S T +    
Sbjct: 5   SLRLPSLTSLLFPATTRYPATL--RRTVCL---RNRP------LSGFATAPSGTASPETK 64

Query: 64  DNSKNRV-PEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVVE 123
            +  +R+  + A+TPRSQDFNAWYLDVIA+AELADYGPVRGTMVIRPYGYAIWEAIQ   
Sbjct: 65  SSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQ--- 124

Query: 124 AIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLV 183
               +YLNVKFKETGH NMYFPQFIPYSFIEKEASHVEGFSPELALVT+GGGKELEEKLV
Sbjct: 125 ----DYLNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLV 184

Query: 184 VRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLE 243
                       VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLE
Sbjct: 185 ------------VRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLE 244

Query: 244 FLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIE 303
           FLWQEGHTAH +PEEAEKEA QMI+IYT+FA+EQ AIPVI GRKSK+ETFAGAD TYTIE
Sbjct: 245 FLWQEGHTAHATPEEAEKEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIE 304

Query: 304 AMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGD 363
           AMMGDRKALQAGTSHNLGQNFSRAFGTQF DENG+RQHVWQTSWA+STRFVGGIIMTHGD
Sbjct: 305 AMMGDRKALQAGTSHNLGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGD 364

Query: 364 DGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESEQRTPGW 423
           D GLMLPPK+APIQVVI+PIWKKD +K  VLSAASSVK +LQTAG++VKLD+++QRTPGW
Sbjct: 365 DTGLMLPPKIAPIQVVIVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGW 424

Query: 424 KFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDK 483
           KFNFWEMKG+PLRIEIGP+DV+S SVV+SRRD+PGK GKVFGISMEPS L AYVK+ LD+
Sbjct: 425 KFNFWEMKGIPLRIEIGPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDE 484

Query: 484 IQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEETGATIRC 543
           IQ+SLLE+A +FRDSNIVDV SY ELK+AIS GKWARGPWSASD DE RVKEETGATIRC
Sbjct: 485 IQTSLLEKALSFRDSNIVDVNSYAELKDAISSGKWARGPWSASDADEQRVKEETGATIRC 543

Query: 544 FPFEQPQGPKTCLMTGNAAEEVAIFAKSY 572
           FPFEQ QG KTCLMTGN AEEVAIFAKSY
Sbjct: 545 FPFEQTQGTKTCLMTGNPAEEVAIFAKSY 543

[Close]

BLAST of Spg002527 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 3.9e-105
Identity = 203/529 (38.37%), Postives = 315/529 (59.55%), Query Frame = 0


Query: 52  ARSSSTGTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYG 111
           A++SS+G + +  + K     G    + ++F  WY +V    ++ +Y  + G  ++RP+ 
Sbjct: 30  AKASSSGQKKK--DVKKETGLGLSVKKDENFGEWYSEV-CKQDMIEYYDISGCYILRPWS 89

Query: 112 YAIWEAIQVVEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTI 171
            AIWE +Q+       + + + K+   +N YFP F+    +EKE  H+EGF+PE+A VT 
Sbjct: 90  MAIWEIMQI-------FFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTK 149

Query: 172 GGGKELEEKLVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWE 231
            G  +LE             P  +RPTSET++   +++WI  +RDLPL +NQW NV RWE
Sbjct: 150 SGKSDLEV------------PIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWE 209

Query: 232 M-RTKPFVRTLEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVE 291
                PF+R+ EFLWQEGHTA  +  EA++E +Q++++Y +   E  A+PV+ G KS+ E
Sbjct: 210 FSNPTPFIRSREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENE 269

Query: 292 TFAGADKTYTIEAMMGDR-KALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAIS 351
            FAG   T ++EA + +  + +Q  TSH LGQNF++ F   F +E  + + VWQ SWA S
Sbjct: 270 KFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYS 329

Query: 352 TRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIK 411
           TR +G +IMTHGDD GL+LPPK+A +QVV+IP+  KD +   +  A ++   +L  AGI+
Sbjct: 330 TRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIR 389

Query: 412 VKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEP 471
            + D  +  +PGWK++ WEMKGVPLRIEIGP+D+ +  V   RRD   K+    G  +E 
Sbjct: 390 AEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVE- 449

Query: 472 SILEAYVKDTLDKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDE 531
                +VK+ L+KIQ ++ E AK  R++ + +V +++E  +A+++ K    PW   ++ E
Sbjct: 450 -----HVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVE 509

Query: 532 ----LRVKEETGAT-IRCFPFEQPQGPK--TCLMTGNAAEEVAIFAKSY 572
                R K ETGA    C PF+QP+ P+   C  +G  A++   + +SY
Sbjct: 510 RDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]

BLAST of Spg002527 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 3.9e-105
Identity = 203/529 (38.37%), Postives = 315/529 (59.55%), Query Frame = 0


Query: 52  ARSSSTGTEIRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYG 111
           A++SS+G + +  + K     G    + ++F  WY +V    ++ +Y  + G  ++RP+ 
Sbjct: 30  AKASSSGQKKK--DVKKETGLGLSVKKDENFGEWYSEV-CKQDMIEYYDISGCYILRPWS 89

Query: 112 YAIWEAIQVVEAIFSEYLNVKFKETGHQNMYFPQFIPYSFIEKEASHVEGFSPELALVTI 171
            AIWE +Q+       + + + K+   +N YFP F+    +EKE  H+EGF+PE+A VT 
Sbjct: 90  MAIWEIMQI-------FFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTK 149

Query: 172 GGGKELEEKLVVRRLSKAIGPFPVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWE 231
            G  +LE             P  +RPTSET++   +++WI  +RDLPL +NQW NV RWE
Sbjct: 150 SGKSDLEV------------PIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWE 209

Query: 232 M-RTKPFVRTLEFLWQEGHTAHVSPEEAEKEAIQMIDIYTKFAYEQAAIPVIAGRKSKVE 291
                PF+R+ EFLWQEGHTA  +  EA++E +Q++++Y +   E  A+PV+ G KS+ E
Sbjct: 210 FSNPTPFIRSREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENE 269

Query: 292 TFAGADKTYTIEAMMGDR-KALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAIS 351
            FAG   T ++EA + +  + +Q  TSH LGQNF++ F   F +E  + + VWQ SWA S
Sbjct: 270 KFAGGLYTTSVEAFIPNTGRGVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYS 329

Query: 352 TRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKGSLQTAGIK 411
           TR +G +IMTHGDD GL+LPPK+A +QVV+IP+  KD +   +  A ++   +L  AGI+
Sbjct: 330 TRTIGVMIMTHGDDKGLVLPPKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIR 389

Query: 412 VKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEP 471
            + D  +  +PGWK++ WEMKGVPLRIEIGP+D+ +  V   RRD   K+    G  +E 
Sbjct: 390 AEEDLRDNYSPGWKYSDWEMKGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVE- 449

Query: 472 SILEAYVKDTLDKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDE 531
                +VK+ L+KIQ ++ E AK  R++ + +V +++E  +A+++ K    PW   ++ E
Sbjct: 450 -----HVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVE 509

Query: 532 ----LRVKEETGAT-IRCFPFEQPQGPK--TCLMTGNAAEEVAIFAKSY 572
                R K ETGA    C PF+QP+ P+   C  +G  A++   + +SY
Sbjct: 510 RDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASGKPAKKWTYWGRSY 530

[Close]

BLAST of Spg002527 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 134.8 bits (338), Expect = 2.2e-31
Identity = 87/227 (38.33%), Postives = 123/227 (54.19%), Query Frame = 0


Query: 358 MTHGDDGGLMLPPKLAPIQVVIIPI-WKKDGDKAEVLSAASSVKGSLQTAGIKVKLDESE 417
           MTHGDD GL+ PPK+AP+QVV+I +  K   D  E+  A  +V+ +L  AGI+ + D  +
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 418 QRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSILEAYV 477
             + GWK+   E+ GVPLRIE GP+D+A+  V I  RD   K     G  +E       V
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIE------QV 208

Query: 478 KDTLDKIQSSLLEQAKTFRDSNIVDVTSYNELKEAISQGKWARGPWSASDKDELRVKEET 537
           KD L+KIQS+L + AK   +     V +++E  EA+SQ K    PW    + E  VK  T
Sbjct: 209 KDLLEKIQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDKVEVEKDVKRRT 268

Query: 538 ----------GATIRCFPFEQPQ-GPKT-CLMTGNAAEEVAIFAKSY 572
                     GA   C P EQP+ G +T C  +G  A++ + + +SY
Sbjct: 269 RGDETGGGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309

The following BLAST results are available for this feature:

BLAST of Spg002527 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878181.1	1.6e-302	91.94	proline--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]	[more]
XP_023541480.1	4.3e-300	91.59	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo]	[more]
XP_022945172.1	1.7e-299	91.24	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata]	[more]
XP_022968367.1	1.1e-295	90.72	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima]	[more]
XP_004149956.3	4.4e-292	88.85	proline--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >XP_03173867...	[more]

BLAST of Spg002527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYR6	7.1e-253	77.68	Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q1CXV1	7.5e-146	51.50	Proline--tRNA ligase OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=proS PE=...	[more]
A9W9Q2	3.7e-145	50.00	Proline--tRNA ligase OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / ...	[more]
B9LC55	3.7e-145	50.00	Proline--tRNA ligase OS=Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / ...	[more]
A7NKX0	4.9e-145	49.40	Proline--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=38...	[more]

BLAST of Spg002527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G067	8.0e-300	91.24	Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111449493 PE=3 SV=1	[more]
A0A6J1HTC6	5.4e-296	90.72	Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111467631 PE=3 SV=1	[more]
A0A6J1C8A3	3.7e-289	88.79	Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1	[more]
A0A1S3C2J3	4.7e-276	91.00	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496137 PE=3 SV=1	[more]
A0A0A0L6I6	1.6e-271	88.19	Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G213510 PE=3 SV=1	[more]

BLAST of Spg002527 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52520.1	5.0e-254	77.68	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.1	3.9e-105	38.37	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.2	3.9e-105	38.37	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	2.2e-31	38.33	tRNA synthetase-related / tRNA ligase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016061	Proline-tRNA ligase, class II, C-terminal	SMART	SM00946	ProRS_C_1_2	coord: 505..571 e-value: 1.6E-26 score: 104.1
IPR016061	Proline-tRNA ligase, class II, C-terminal	PFAM	PF09180	ProRS-C_1	coord: 505..571 e-value: 5.4E-18 score: 65.1
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 76..369 e-value: 7.7E-101 score: 339.2
None	No IPR available	PANTHER	PTHR43382:SF7	BNAC09G28510D PROTEIN	coord: 2..571
None	No IPR available	CDD	cd00862	ProRS_anticodon_zinc	coord: 365..571 e-value: 2.26214E-83 score: 256.841
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 365..497
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 81..363
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 370..496 e-value: 3.3E-34 score: 119.2
IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	PFAM	PF00587	tRNA-synt_2b	coord: 195..357 e-value: 1.7E-18 score: 67.2
IPR017449	Prolyl-tRNA synthetase, class II	GENE3D	3.30.110.30		coord: 500..571 e-value: 1.5E-20 score: 74.8
IPR017449	Prolyl-tRNA synthetase, class II	SUPERFAMILY	64586	C-terminal domain of ProRS	coord: 499..571
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 376..455 e-value: 5.6E-16 score: 58.5
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	TIGRFAM	TIGR00408	TIGR00408	coord: 76..571 e-value: 8.2E-156 score: 517.5
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	PANTHER	PTHR43382	PROLYL-TRNA SYNTHETASE	coord: 2..571
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	HAMAP	MF_01571	Pro_tRNA_synth_type3	coord: 75..571 score: 41.282742
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 133..369 score: 19.45228
IPR033721	Prolyl-tRNA synthetase, catalytic domain	CDD	cd00778	ProRS_core_arch_euk	coord: 81..359 e-value: 2.02705E-163 score: 463.991

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002527.1	Spg002527.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009553	embryo sac development
biological_process	GO:0048481	plant ovule development
biological_process	GO:0006433	prolyl-tRNA aminoacylation
biological_process	GO:0010109	regulation of photosynthesis
biological_process	GO:0048316	seed development
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding