Spg002488 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002488
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSpermidine synthase
Locationscaffold6: 784422 .. 788702 (+)
RNA-Seq ExpressionSpg002488
SyntenySpg002488
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGAGTGGAACGAAAGAAGAAGAAGACGGGAGTGAAGAAAGAAAAAAAAAAAGAACTTCCTTTTGAGTCGAATTCGTACTCACTATCGAAAGCTGTAAAACCCAATTTTGATTTTGTGGTTTTGTTTTGTTTGTGGTCAAATTTAGTGTTCATTTCCATCTGCAACTCGAATCCCACTGCGCATTCATCTATATAAACCAACGCCATTTTTGGGCAAACCAAGCTCCAGCCCTTGTGACCAAGAGGGACTGAAATTTCTCACAACGGACTTCTGGGGTCTTTGATTTTTCTCTTCACGTTCGCTTCGCCATTCTTCGACCTTTCACTGTAATCTTTCAATCCCTTTTTCTCTTTCGTTTTCAATCTGTTCTTATTTTTCTTTCTCTGTTCTTTACTAGTTTCTGTGTTCGTCCTCTCTCTTCTGATGTTCTGTACGTATGTTTCTGAAATGGGGTTTGAAAAACTTGATCAGTGATGTTGGGGTTTTGGGTTTTATTCGTTTTCCGTTTCCACCAGAAGGATCTCGAAGTGGTCTGTCACAGGAGGCTCTGGTTTTTGTTTTTCTTTTAATTCTCGTGTCATTTCTTAACCCTGCAGATTTTCAGGAAAAGAACAAATGAAGGAAGAGTTTGAGGCACTTTTTTAGAGCTAAAAAATTAAACATAGATGGAAATATTTGGATTTGGCCTCTTTTATTAGTCGGTGTGTGTAAGATAGAGATACACGTTTCCTTATTTTGTCCCTTTCTGGTTTGGCAAATTTGTTTTCCATCTTTAATCTCTTGTTGAACAACCTACTCATAAACAATAGTTTATGGCTGATATGCTGGTGAAACTGAAACTGAAACTGATAGATAGTGGGGAATCTGAACTTCTTTTTCATTTTAAAATAAATTTCTCTTATTATTTTGCACGTTTAGTCTAGTTGAGCTACGTGAGTTAATAGAAGGTTTTTGATGAACAGTTCTGGCTTATGGAAATAGTACTGCTCTGCTCGTGCTAGTATAGTTATGTCCCTGATTTCATTTCTGAGGATTTTTCTATTCCCCATGACTTTCATGTGCTTTTTCCTTTTTCATTCGAAGGGTCTGAAACTCTATTCTGATGCATTTTAGGCATATCATTTGCTTATTTCTCATTACTGCCTTTGTGAAACTCCATCCTTTGTCCTTGTCATTTTCTTCCCCATCTTTTCATGTGAATTGGTGAGTTTTCTGGTCCTAGCAATTTCAAACCTGGAAGAACTCGAAAGTTACTGAGAGATCCTTTATTTTCTCTATTTCTAGATTTAACATAATTCTGATAGCATTCTTCACTCGGGTAATTTTTCAGTTTTTTGTTGATCCTAAGCTTTGCATTATACACTATAGAAAAAGATGCGTGGCTGTGTTTATTGAGTTGTGATCTGCGTTTCTGTAAATTTCTTGCTTCTTTTAGTAGAAGGCTGCTGCCTCTGCTAACTGGATGGATTGCATTATGGCTTGTGCTGTTCCTTCCTTCTTTGTTTTTACAGCTGATTATTCTTCGATCTTATTTTATGGGAATTCCTTGGTAAAGATTTTTGTCTTTTCTTGATGGTGTTTAACAAAGCATATTAGTTGGTGGCATGTATTTGTCCAACAGCTAAGAAACTTATAACAAAGTTATATGTTCTATAATTTTATTTGTTCACTGCTGTTTTCAATTTCTTCCTCTGAAATCATGAATCTGCAGAAAGTAAAGACGCAAAGGCTTTCAAAATGTATGATCTATTATTTTGCCACAACTTATGTACGGTAGAAGTGCATGAGTTACTTGAAAATTTTTCTTGTACGCATATTTCCTTGCTCCATCTTTCTGATGATGCGTTATTCTTGATGTTCCAGAGGCTGGAGAAAGAAGAGAAAAAGATAATACATGGAGGACGTCACAGGAAGAAGTTTGGAATGCCAGAAGATTATGGATGGGAAGGCGACTAATGGGAATGGTTTAGAGAAGGCCATTCCTTCTTGTTGCTTGAAGGCTAAGACTTCAGGCCCTGAGCTTGAGGCTCAATGCCATTCAACTGTTGTTTCTGGCTGGTTTTCAGACTTTCAGTCAGGCTCTGGTCATTCTCATTCTCCTTTTCTTTAATCTTCTTCTGAAGCTACTGGCTCTGTTTTAGACACCATGGCTTATCATGTGGCTGATTAACACCACTTGCACTTGTTTCTTTTGCAGATGAAGCTGGCAAGGAAGTTTATTTCAACAACCCAATGTGGCCGGGTGAGTAGCTTTCTTATATTTCATGTTAATGCTCTCCTTTTCATGCTGATTCTGAAAAAGGAATTGAATGGTCATAAAAACAGTTCATGACAATTTTTGCCACATCGAAACTCTGTTAATGTCCATTTGATTGCATTAGTGTTATGTAAGTGACGCATCAAATTTCTGCATTAAAGCGGATGTAATTGTCTCCCTCTCAGGAGAAGCACATGCTCTGCAAGTAAAGAATATACTATTCAAAGGAAAATCAGAGTACCAAGAGGTTATGGTTTTTGAGGTACAATCCGCAGACCCGATGTCTTTCTTTTGCAAATTGTTTGCTTGGTTTCTTGACTAATACCTGATCTGTTTGTCTGTATTTGTTTACAGTCTGCATCATATGGGAAAGTCCTTGTTCTTGATGGCATTGTTCAACTGACAGAGAAAGATGAATGTGCCTACCAAGAAATGATAGCGCACCTTCCTCTTTGTTCAATCCCATCTCCAAAAACCGTTAGTTCTCTCAAAAAATTGCTTCTGCACCTATCTAACTCTTATTGAGAAATTTTGTTTTCTCCTCCAACTTTTGCATAATTAAGACATAAAATTGCACTATATAGGTTCTGGTTGTTGGCGGAGGTGATGGGGGAGTTCTGAGGGAAATTTCTCGTCACGACTCAGTGGAGCACATTGACATTTGTGAGATAGATAAGATGGTCATTGATGTAAGTACATAGAGAATTGTTCATTAGACGACATGTTGCGAATTGTATGATAAGTTCATAATGAAATAACTTTCTGTAGTGGTATGTTTCAAGAGTGTAATGTAATGACTTGTTTCCCTGCTTGTATCTGTATGCTAGGTGTCAAAAAAGTTTTTTCCCCAACTAGCTGTTGGATTTGAGGACCCCCGTGTCCACCTTCATGTTGGTGATGGTACGTTGAAAGTCGAGACATCGTGTTGCTTATTTGTTTCTTTTGCCCATCTCTGTATAATTTGTTTAATCTTTGTAATATCAGCTGTTGAATTCCTTCGGAATGCAAAGCCAGGGAAGTATGATGCCATCATTGTTGATTCATCTGATCCTGTGGGTAAGTTTTTGAATTGTAGCAGATAGTAGGATCAAAAGAAATGGTGCAACTCTGCAAGCTACATACATATCTTTTAGTTTAACAATTAGAACACCTTAAAATTATGTTCTCTATCAATAAAACTTGTGCTTGTAAACTCATCTTCTAACCGGGGTTCCTCGTAATGTAGGTCCTGCCCAAGAGCTGGTGGAGATGCCATTCTTTCGGACAATAGCCCGAGCATTACGACCGGGTGGCGTCCTCTGTAACATGGCTGAAAGTATGTGGCTGCATACACATCTTATTCAGGACATGATATTGATTTGTCGCCAAACGTTCAAGGATGTTCACTATGCATGGACCAGTGTTCCAACATATCCAAGTGGTGTCATAGGGTTTCTGCTTTGCTCAACTGAAGGGCCAACAGTTGATTTCAAGAACCCTGTGAATCCCATTGAGAAGTTGGGACATCCCAAGTCTAAAAGAGAACTGAAATTCTACAACTCCGAGGTAACAACACGAAAACTCTCTAAATGGCGACGCCATTCTCTCTGTATGTTTCGAATTCGATACGATGTCGATGTTAGAGATGCATCCATTGACATTACGAATTAAAACTAGTGTCATGTTCTATGTTCATACTATTAATGGTTGCATTCCAACAGATGCACTCGGCTGCATTCGCGCTGCCATCATTTCTGAAGAAGGAGGTGAAAGCGCTTAGCGACGCCCCGGCATCAGGGAAAAAAGGATGCATTTCATCCTAAGTATATGGCAGTTTGAAATGCTTTTTACCAAGGTGGTGATGGATATTATTGTTCAGTGTATACATAAAAGTATTGGAGTGGCTGAGACATCTCTAGTGTGTACTGAGGGAATTGTTAATGACTACTATTTAATAAATTCAGGTTTGGTATCCTATTACAAGAAAAATGTTTGGTTCTTTTTGTTGTTTTTTCATGGCTGATTGGTATGCCAATTAGCTGAT

mRNA sequence

ATGGAGGACGTCACAGGAAGAAGTTTGGAATGCCAGAAGATTATGGATGGGAAGGCGACTAATGGGAATGGTTTAGAGAAGGCCATTCCTTCTTGTTGCTTGAAGGCTAAGACTTCAGGCCCTGAGCTTGAGGCTCAATGCCATTCAACTGTTGTTTCTGGCTGGTTTTCAGACTTTCAGTCAGGCTCTGATGAAGCTGGCAAGGAAGTTTATTTCAACAACCCAATGTGGCCGGCGGATGTAATTGTCTCCCTCTCAGGAGAAGCACATGCTCTGCAAGTAAAGAATATACTATTCAAAGGAAAATCAGAGTACCAAGAGGTTATGGTTTTTGAGTCTGCATCATATGGGAAAGTCCTTGTTCTTGATGGCATTGTTCAACTGACAGAGAAAGATGAATGTGCCTACCAAGAAATGATAGCGCACCTTCCTCTTTGTTCAATCCCATCTCCAAAAACCGTTCTGGTTGTTGGCGGAGGTGATGGGGGAGTTCTGAGGGAAATTTCTCGTCACGACTCAGTGGAGCACATTGACATTTGTGAGATAGATAAGATGGTCATTGATGTGTCAAAAAAGTTTTTTCCCCAACTAGCTGTTGGATTTGAGGACCCCCGTGTCCACCTTCATGTTGGTGATGCTGTTGAATTCCTTCGGAATGCAAAGCCAGGGAAGTATGATGCCATCATTGTTGATTCATCTGATCCTGTGGGTCCTGCCCAAGAGCTGGTGGAGATGCCATTCTTTCGGACAATAGCCCGAGCATTACGACCGGGTGGCGTCCTCTGTAACATGGCTGAAAGTATGTGGCTGCATACACATCTTATTCAGGACATGATATTGATTTGTCGCCAAACGTTCAAGGATGTTCACTATGCATGGACCAGTGTTCCAACATATCCAAGTGGTGTCATAGGGTTTCTGCTTTGCTCAACTGAAGGGCCAACAGTTGATTTCAAGAACCCTGTGAATCCCATTGAGAAGTTGGGACATCCCAAGTCTAAAAGAGAACTGAAATTCTACAACTCCGAGATGCACTCGGCTGCATTCGCGCTGCCATCATTTCTGAAGAAGGAGGTGAAAGCGCTTAGCGACGCCCCGGCATCAGGGAAAAAAGGATGCATTTCATCCTAA

Coding sequence (CDS)

ATGGAGGACGTCACAGGAAGAAGTTTGGAATGCCAGAAGATTATGGATGGGAAGGCGACTAATGGGAATGGTTTAGAGAAGGCCATTCCTTCTTGTTGCTTGAAGGCTAAGACTTCAGGCCCTGAGCTTGAGGCTCAATGCCATTCAACTGTTGTTTCTGGCTGGTTTTCAGACTTTCAGTCAGGCTCTGATGAAGCTGGCAAGGAAGTTTATTTCAACAACCCAATGTGGCCGGCGGATGTAATTGTCTCCCTCTCAGGAGAAGCACATGCTCTGCAAGTAAAGAATATACTATTCAAAGGAAAATCAGAGTACCAAGAGGTTATGGTTTTTGAGTCTGCATCATATGGGAAAGTCCTTGTTCTTGATGGCATTGTTCAACTGACAGAGAAAGATGAATGTGCCTACCAAGAAATGATAGCGCACCTTCCTCTTTGTTCAATCCCATCTCCAAAAACCGTTCTGGTTGTTGGCGGAGGTGATGGGGGAGTTCTGAGGGAAATTTCTCGTCACGACTCAGTGGAGCACATTGACATTTGTGAGATAGATAAGATGGTCATTGATGTGTCAAAAAAGTTTTTTCCCCAACTAGCTGTTGGATTTGAGGACCCCCGTGTCCACCTTCATGTTGGTGATGCTGTTGAATTCCTTCGGAATGCAAAGCCAGGGAAGTATGATGCCATCATTGTTGATTCATCTGATCCTGTGGGTCCTGCCCAAGAGCTGGTGGAGATGCCATTCTTTCGGACAATAGCCCGAGCATTACGACCGGGTGGCGTCCTCTGTAACATGGCTGAAAGTATGTGGCTGCATACACATCTTATTCAGGACATGATATTGATTTGTCGCCAAACGTTCAAGGATGTTCACTATGCATGGACCAGTGTTCCAACATATCCAAGTGGTGTCATAGGGTTTCTGCTTTGCTCAACTGAAGGGCCAACAGTTGATTTCAAGAACCCTGTGAATCCCATTGAGAAGTTGGGACATCCCAAGTCTAAAAGAGAACTGAAATTCTACAACTCCGAGATGCACTCGGCTGCATTCGCGCTGCCATCATTTCTGAAGAAGGAGGTGAAAGCGCTTAGCGACGCCCCGGCATCAGGGAAAAAAGGATGCATTTCATCCTAA

Protein sequence

MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVKALSDAPASGKKGCISS
Homology
BLAST of Spg002488 vs. NCBI nr
Match: XP_038878719.1 (spermine synthase-like [Benincasa hispida])

HSP 1 Score: 701.4 bits (1809), Expect = 4.1e-198
Identity = 345/376 (91.76%), Postives = 355/376 (94.41%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDVTGRSLECQKI DGK TNGNG EKAIPSCC KAKTS PELEAQCHSTVVSGWFS+FQ
Sbjct: 1   MEDVTGRSLECQKIKDGKVTNGNGSEKAIPSCCSKAKTSDPELEAQCHSTVVSGWFSEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
           SGSD+A K+VYFNNPMWP        GEAHALQV+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  SGSDKACKKVYFNNPMWP--------GEAHALQVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPK+VLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKSVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EIDKMVIDVSKK+FPQLAVGFEDPRV LHVGDAVEFLRNAK GKYDAIIVDSSDPVGPAQ
Sbjct: 181 EIDKMVIDVSKKYFPQLAVGFEDPRVLLHVGDAVEFLRNAKQGKYDAIIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFFRTIA+ALRPGGVLCNMAESMWLHTHLIQDMI ICRQTFKDV YAWTSVPTYP
Sbjct: 241 ELVEMPFFRTIAQALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVCYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVK 360
           SGVIGFLLCSTEGP VDF+NPVNPIEKLGH KSKRELKFYNSEMHSAAFALPSFLK+EV+
Sbjct: 301 SGVIGFLLCSTEGPKVDFRNPVNPIEKLGHLKSKRELKFYNSEMHSAAFALPSFLKREVQ 360

Query: 361 ALSDAPASGKKGCISS 377
           ALSDAPASGK GCISS
Sbjct: 361 ALSDAPASGKNGCISS 368

BLAST of Spg002488 vs. NCBI nr
Match: XP_004150312.1 (spermine synthase [Cucumis sativus] >KGN58277.1 hypothetical protein Csa_017516 [Cucumis sativus])

HSP 1 Score: 701.0 bits (1808), Expect = 5.4e-198
Identity = 340/376 (90.43%), Postives = 354/376 (94.15%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDVTG+SLECQKIMDGK TNGNG EK IPSCCLKAKTS PELEAQCHSTVVSGWFS+FQ
Sbjct: 1   MEDVTGKSLECQKIMDGKVTNGNGREKVIPSCCLKAKTSDPELEAQCHSTVVSGWFSEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
           SGSD+A K++YFNNPMWP        GEAHAL+V+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  SGSDKACKKLYFNNPMWP--------GEAHALEVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPK+VLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKSVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EID+MVIDVSKK FPQLAVGFEDPRVHLHVGDAVEFLRN K GKYDAIIVDSSDPVGPAQ
Sbjct: 181 EIDQMVIDVSKKCFPQLAVGFEDPRVHLHVGDAVEFLRNGKQGKYDAIIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFFR IA+ALRPGGVLCNMAESMWLHTHLIQDMI ICRQTFKDVHYAWTSVPTYP
Sbjct: 241 ELVEMPFFRMIAQALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVK 360
           SGVIGFLLCSTEGP VDF NPVNPIE +GHPKSKRELKFYNSEMHSAAFALPSFLK+EV+
Sbjct: 301 SGVIGFLLCSTEGPKVDFSNPVNPIENVGHPKSKRELKFYNSEMHSAAFALPSFLKREVQ 360

Query: 361 ALSDAPASGKKGCISS 377
           AL+DAPASGK GCISS
Sbjct: 361 ALNDAPASGKNGCISS 368

BLAST of Spg002488 vs. NCBI nr
Match: XP_022141267.1 (spermine synthase-like [Momordica charantia])

HSP 1 Score: 698.7 bits (1802), Expect = 2.7e-197
Identity = 345/378 (91.27%), Postives = 354/378 (93.65%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDV GRSLECQKIMDGKATNGNGL+ AIPSCCLKAKTS PELEAQCHSTVVSGWFS+FQ
Sbjct: 1   MEDVAGRSLECQKIMDGKATNGNGLDGAIPSCCLKAKTSDPELEAQCHSTVVSGWFSEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
           SGSD+A K VYFNNPMWP        GEAHALQV+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  SGSDKASKNVYFNNPMWP--------GEAHALQVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNA+ GKYDAIIVDSSDPVGPAQ
Sbjct: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNARQGKYDAIIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFF+TIARALR GGVLCNMAESMWLHTHLIQDMI ICR+TFKDVHYAWTSVPTYP
Sbjct: 241 ELVEMPFFQTIARALRSGGVLCNMAESMWLHTHLIQDMISICRETFKDVHYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKL--GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           SGVIGFLLCSTEGPTVDF+NPVNPIE L   H KSKRELKFYNSEMHSAAFALPSFLK+E
Sbjct: 301 SGVIGFLLCSTEGPTVDFRNPVNPIENLVGQHLKSKRELKFYNSEMHSAAFALPSFLKRE 360

Query: 361 VKALSDAPASGKKGCISS 377
           VKALSDAP SGK G ISS
Sbjct: 361 VKALSDAPVSGKNGWISS 370

BLAST of Spg002488 vs. NCBI nr
Match: XP_022944858.1 (spermine synthase-like [Cucurbita moschata])

HSP 1 Score: 696.0 bits (1795), Expect = 1.7e-196
Identity = 342/376 (90.96%), Postives = 353/376 (93.88%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDVTGRSLECQ IMDGKATNGNGL+KAIPSCCLKAK S PELEAQCHSTVVSGW S+FQ
Sbjct: 1   MEDVTGRSLECQTIMDGKATNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
           SGSD+A K VYFNNPMWP        GEAH+LQV+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  SGSDKASKFVYFNNPMWP--------GEAHSLQVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIP PKTVLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPFPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EID+MVI+VSK+FFPQLAVGFEDPRVHLHVGDAVEFLRNAK GKYDA+IVDSSDPVGPAQ
Sbjct: 181 EIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFF+TIARALRPGGVLCNMAESMWLHTHLIQDMI ICRQTFKDVHYAWTSVPTYP
Sbjct: 241 ELVEMPFFQTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVK 360
           SGVIGFLLCSTEGPTVDF+NPVNPIEKLGH KSKRELKFYNSEMH AAFALPSFLK EVK
Sbjct: 301 SGVIGFLLCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVK 360

Query: 361 ALSDAPASGKKGCISS 377
           ALSDA AS KKG ISS
Sbjct: 361 ALSDASASLKKGSISS 368

BLAST of Spg002488 vs. NCBI nr
Match: KAG7013301.1 (Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 693.7 bits (1789), Expect = 8.6e-196
Identity = 343/377 (90.98%), Postives = 354/377 (93.90%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIM-DGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDF 60
           MEDVTGRSLECQ IM DGKATNGNGL+KAIPSCCLKAK S PELEAQCHSTVVSGW S+F
Sbjct: 1   MEDVTGRSLECQTIMDDGKATNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEF 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
           QSGSD+A K VYFNNPMWP        GEAH+LQV+NILFKGKSEYQEVMVFESASYGKV
Sbjct: 61  QSGSDKASKFVYFNNPMWP--------GEAHSLQVENILFKGKSEYQEVMVFESASYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEID+MVI+VSK+FFPQLAVGFEDPRVHLHVGDAVEFLRNAK GKYDA+IVDSSDPVGPA
Sbjct: 181 CEIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
           QELVEMPFF+TIARALRPGGVLCNMAESMWLHTHLIQDMI ICRQTFKDVHYAWTSVPTY
Sbjct: 241 QELVEMPFFQTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEV 360
           PSGVIGFLLCSTEGPTVDF+NPVNPIEKLGH KSKRELKFYNSEMH AAFALPSFLK EV
Sbjct: 301 PSGVIGFLLCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEV 360

Query: 361 KALSDAPASGKKGCISS 377
           KALSDA AS KKG ISS
Sbjct: 361 KALSDASASLKKGSISS 369

BLAST of Spg002488 vs. ExPASy Swiss-Prot
Match: Q94BN2 (Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 2.2e-162
Identity = 279/364 (76.65%), Postives = 308/364 (84.62%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSD-F 60
           ME   G  L CQ  MDGKA+NGNGLEK +PSCCLKA    PE +A+CHSTVVSGWFS+  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
                + GK VYFNNPMWP        GEAH+L+V+ +LFK KS++QEV+VFESA+YGKV
Sbjct: 61  PRSGKKGGKAVYFNNPMWP--------GEAHSLKVEKVLFKDKSDFQEVLVFESATYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEIDKMVIDVSKKFFP+LAVGF+DPRV LH+GDA EFLR +  GKYDAIIVDSSDPVGPA
Sbjct: 181 CEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
             LVE PFF T+ARAL+PGGVLCNMAESMWLHTHLI+DMI ICRQTFK VHYAW+SVPTY
Sbjct: 241 LALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKL-GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           PSGVIGF+LCSTEGP VDFKNP+NPIEKL G    KRELKFYNS+MH AAFALP+FL++E
Sbjct: 301 PSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRRE 356

Query: 361 VKAL 363
           V +L
Sbjct: 361 VASL 356

BLAST of Spg002488 vs. ExPASy Swiss-Prot
Match: O82147 (Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.0e-122
Identity = 210/313 (67.09%), Postives = 253/313 (80.83%), Query Frame = 0

Query: 49  STVVSGWFSDFQSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEV 108
           S+V+ GWFS+               +PMWP        GEAH+L+V+ ILF+GKS+YQ V
Sbjct: 21  SSVIPGWFSEI--------------SPMWP--------GEAHSLKVEKILFQGKSDYQNV 80

Query: 109 MVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREI 168
           MVF+S++YGKVLVLDG++QLTE+DECAYQEMIAHLPLCSIPSPK VLV+GGGDGGVLRE+
Sbjct: 81  MVFQSSTYGKVLVLDGVIQLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREV 140

Query: 169 SRHDSVEHIDICEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAI 228
           +RH SVE IDICEIDKMV+DVSK+FFP +AVGFEDPRV LH+GD V FL+    G YDAI
Sbjct: 141 ARHLSVEQIDICEIDKMVVDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAI 200

Query: 229 IVDSSDPVGPAQELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFK- 288
           IVDSSDP+GPAQEL E PFF ++A+ALRPGGV+C  AES+WLH H+I+D++  CRQ FK 
Sbjct: 201 IVDSSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKG 260

Query: 289 DVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFKNPVNPIE-KLGHPKSKRELKFYNSEMHS 348
            V+YAWT+VPTYPSGVIGF+LCSTEGP VDFK+P+NPI+   G  K+ + LKFYNSE+HS
Sbjct: 261 SVNYAWTTVPTYPSGVIGFMLCSTEGPPVDFKHPINPIDANDGRSKTMKPLKFYNSEIHS 311

Query: 349 AAFALPSFLKKEV 360
           AAF LPSF KK +
Sbjct: 321 AAFCLPSFAKKVI 311

BLAST of Spg002488 vs. ExPASy Swiss-Prot
Match: Q9ZUB3 (Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 2.2e-122
Identity = 209/354 (59.04%), Postives = 271/354 (76.55%), Query Frame = 0

Query: 15  MDGKATNGNGLEKAIP------SCCLKAKTSGPELEAQCHSTVVSGWFSDFQSGSDEAGK 74
           MD K T+   L++         +  ++ +    + E  C STV+ GWFS+          
Sbjct: 1   MDAKETSATDLKRPREEDDNGGAATMETENGDQKKEPACFSTVIPGWFSEM--------- 60

Query: 75  EVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 134
                +PMWP        GEAH+L+V+ +LF+GKS+YQ+V+VF+SA+YGKVLVLDG++QL
Sbjct: 61  -----SPMWP--------GEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQL 120

Query: 135 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDKMVID 194
           TE+DECAYQEMI HLPLCSIP+PK VLV+GGGDGGVLRE++RH S+E ID+CEIDKMV+D
Sbjct: 121 TERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVD 180

Query: 195 VSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQELVEMPFF 254
           VSK+FFP +A+G+EDPRV+L +GD V FL+NA  G YDA+IVDSSDP+GPA+EL E PFF
Sbjct: 181 VSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPIGPAKELFEKPFF 240

Query: 255 RTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFK-DVHYAWTSVPTYPSGVIGFL 314
           +++ARALRPGGV+C  AES+WLH  +I+D++  CR+ FK  V+YAWTSVPTYPSGVIGF+
Sbjct: 241 QSVARALRPGGVVCTQAESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFM 300

Query: 315 LCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVKA 362
           LCSTEGP VDFK+P+NPI++    KS   LKFYN+E+HSAAF LPSF KK +++
Sbjct: 301 LCSTEGPDVDFKHPLNPIDE-SSSKSNGPLKFYNAEIHSAAFCLPSFAKKVIES 331

BLAST of Spg002488 vs. ExPASy Swiss-Prot
Match: Q96557 (Spermidine synthase 2 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.9e-122
Identity = 203/318 (63.84%), Postives = 255/318 (80.19%), Query Frame = 0

Query: 44  EAQCHSTVVSGWFSDFQSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKS 103
           E+ C S+V+ GWFS+               +P+WP        GEAH+L+V+ ILF+GKS
Sbjct: 18  ESPCISSVLPGWFSEI--------------SPLWP--------GEAHSLKVEKILFQGKS 77

Query: 104 EYQEVMVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGG 163
           +YQ+VMVF+S +YGKVLVLDG++QLTE+DECAYQEMI HLPLCSIP+PK VLV+GGGDGG
Sbjct: 78  DYQDVMVFQSTTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGG 137

Query: 164 VLREISRHDSVEHIDICEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPG 223
           VLRE+SRH SVE IDICEIDKMV++V+K+FFP +AVG+EDPRV+LH+GD V FL+N   G
Sbjct: 138 VLREVSRHSSVEQIDICEIDKMVVEVAKQFFPDVAVGYEDPRVNLHIGDGVAFLKNVPAG 197

Query: 224 KYDAIIVDSSDPVGPAQELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICR 283
            YDA+IVDSSDP+GPAQEL E PFF TIARALRPGGV+   AES+WLH H+I++++  CR
Sbjct: 198 TYDAVIVDSSDPIGPAQELFEKPFFETIARALRPGGVVSTQAESIWLHMHIIEEIVANCR 257

Query: 284 QTFK-DVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNS 343
           + FK  V+YAWT+VPTYPSG+IGF+LCSTEGP VDFKNP+NPI+   H ++   LKFYNS
Sbjct: 258 EIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFKNPINPIDDESHGQTIGPLKFYNS 313

Query: 344 EMHSAAFALPSFLKKEVK 361
           E+H A+F LPSF K+ ++
Sbjct: 318 EIHQASFCLPSFAKRVIE 313

BLAST of Spg002488 vs. ExPASy Swiss-Prot
Match: Q96556 (Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 5.0e-122
Identity = 204/315 (64.76%), Postives = 254/315 (80.63%), Query Frame = 0

Query: 49  STVVSGWFSDFQSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEV 108
           S+++ GWFS+               +P+WP        GEAH+L+V+ ILF+GKS+YQ V
Sbjct: 13  SSILPGWFSEI--------------SPLWP--------GEAHSLKVEKILFQGKSDYQNV 72

Query: 109 MVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREI 168
           MVF+S++YGKVL+LDG++QLTE+DECAYQEMI HLPLCSIP+PK VLV+GGGDGGVLRE+
Sbjct: 73  MVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREV 132

Query: 169 SRHDSVEHIDICEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAI 228
           SRH SVE IDICEIDKMVIDVSK+FFP +A+G+EDPRV LHVGD V FL+    G YDA+
Sbjct: 133 SRHSSVEQIDICEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKFVAEGTYDAV 192

Query: 229 IVDSSDPVGPAQELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFK- 288
           IVDSSDP+GPAQEL E PFF ++ARALRPGGV+C  AES+WLH H+I+D++  CRQ FK 
Sbjct: 193 IVDSSDPIGPAQELFEKPFFESVARALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKG 252

Query: 289 DVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFKNPVNPIE-KLGHPKSKRELKFYNSEMHS 348
            V+YAWT+VPTYPSGVIGF+LCSTEGP VDFKNP+NP++    H K++  LKFYNSE+HS
Sbjct: 253 SVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKNPINPVDADDSHTKTRGPLKFYNSEIHS 305

Query: 349 AAFALPSFLKKEVKA 362
           A+F LPSF K+ +++
Sbjct: 313 ASFCLPSFAKRVIES 305

BLAST of Spg002488 vs. ExPASy TrEMBL
Match: A0A0A0LC47 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G604620 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.6e-198
Identity = 340/376 (90.43%), Postives = 354/376 (94.15%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDVTG+SLECQKIMDGK TNGNG EK IPSCCLKAKTS PELEAQCHSTVVSGWFS+FQ
Sbjct: 1   MEDVTGKSLECQKIMDGKVTNGNGREKVIPSCCLKAKTSDPELEAQCHSTVVSGWFSEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
           SGSD+A K++YFNNPMWP        GEAHAL+V+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  SGSDKACKKLYFNNPMWP--------GEAHALEVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPK+VLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKSVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EID+MVIDVSKK FPQLAVGFEDPRVHLHVGDAVEFLRN K GKYDAIIVDSSDPVGPAQ
Sbjct: 181 EIDQMVIDVSKKCFPQLAVGFEDPRVHLHVGDAVEFLRNGKQGKYDAIIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFFR IA+ALRPGGVLCNMAESMWLHTHLIQDMI ICRQTFKDVHYAWTSVPTYP
Sbjct: 241 ELVEMPFFRMIAQALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVK 360
           SGVIGFLLCSTEGP VDF NPVNPIE +GHPKSKRELKFYNSEMHSAAFALPSFLK+EV+
Sbjct: 301 SGVIGFLLCSTEGPKVDFSNPVNPIENVGHPKSKRELKFYNSEMHSAAFALPSFLKREVQ 360

Query: 361 ALSDAPASGKKGCISS 377
           AL+DAPASGK GCISS
Sbjct: 361 ALNDAPASGKNGCISS 368

BLAST of Spg002488 vs. ExPASy TrEMBL
Match: A0A6J1CJE6 (spermine synthase-like OS=Momordica charantia OX=3673 GN=LOC111011708 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.3e-197
Identity = 345/378 (91.27%), Postives = 354/378 (93.65%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDV GRSLECQKIMDGKATNGNGL+ AIPSCCLKAKTS PELEAQCHSTVVSGWFS+FQ
Sbjct: 1   MEDVAGRSLECQKIMDGKATNGNGLDGAIPSCCLKAKTSDPELEAQCHSTVVSGWFSEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
           SGSD+A K VYFNNPMWP        GEAHALQV+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  SGSDKASKNVYFNNPMWP--------GEAHALQVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNA+ GKYDAIIVDSSDPVGPAQ
Sbjct: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNARQGKYDAIIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFF+TIARALR GGVLCNMAESMWLHTHLIQDMI ICR+TFKDVHYAWTSVPTYP
Sbjct: 241 ELVEMPFFQTIARALRSGGVLCNMAESMWLHTHLIQDMISICRETFKDVHYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKL--GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           SGVIGFLLCSTEGPTVDF+NPVNPIE L   H KSKRELKFYNSEMHSAAFALPSFLK+E
Sbjct: 301 SGVIGFLLCSTEGPTVDFRNPVNPIENLVGQHLKSKRELKFYNSEMHSAAFALPSFLKRE 360

Query: 361 VKALSDAPASGKKGCISS 377
           VKALSDAP SGK G ISS
Sbjct: 361 VKALSDAPVSGKNGWISS 370

BLAST of Spg002488 vs. ExPASy TrEMBL
Match: A0A6J1FZ77 (spermine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111449258 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.4e-197
Identity = 342/376 (90.96%), Postives = 353/376 (93.88%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDVTGRSLECQ IMDGKATNGNGL+KAIPSCCLKAK S PELEAQCHSTVVSGW S+FQ
Sbjct: 1   MEDVTGRSLECQTIMDGKATNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
           SGSD+A K VYFNNPMWP        GEAH+LQV+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  SGSDKASKFVYFNNPMWP--------GEAHSLQVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIP PKTVLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPFPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EID+MVI+VSK+FFPQLAVGFEDPRVHLHVGDAVEFLRNAK GKYDA+IVDSSDPVGPAQ
Sbjct: 181 EIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFF+TIARALRPGGVLCNMAESMWLHTHLIQDMI ICRQTFKDVHYAWTSVPTYP
Sbjct: 241 ELVEMPFFQTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVK 360
           SGVIGFLLCSTEGPTVDF+NPVNPIEKLGH KSKRELKFYNSEMH AAFALPSFLK EVK
Sbjct: 301 SGVIGFLLCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVK 360

Query: 361 ALSDAPASGKKGCISS 377
           ALSDA AS KKG ISS
Sbjct: 361 ALSDASASLKKGSISS 368

BLAST of Spg002488 vs. ExPASy TrEMBL
Match: A0A6J1HVE7 (spermine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111467782 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.7e-195
Identity = 340/376 (90.43%), Postives = 350/376 (93.09%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDFQ 60
           MEDVTGRS ECQ IMDGK TNGNGL+KAIPSCCLKAK S PELEAQCHSTVVSGW S+FQ
Sbjct: 1   MEDVTGRSFECQTIMDGKTTNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKVL 120
            GSD+A K VYFNNPMWP        GEAH+L V+NILFKGKSEYQEVMVFESASYGKVL
Sbjct: 61  PGSDKASKFVYFNNPMWP--------GEAHSLLVENILFKGKSEYQEVMVFESASYGKVL 120

Query: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180
           VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC
Sbjct: 121 VLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDIC 180

Query: 181 EIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPAQ 240
           EID+MVI+VSK+FFPQLAVGFEDPRVHLHVGDAVEFLRNAK GKYDAIIVDSSDPVGPAQ
Sbjct: 181 EIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAIIVDSSDPVGPAQ 240

Query: 241 ELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTYP 300
           ELVEMPFF+TIARALRPGGVLCNMAESMWLHTHLIQDMI ICRQTFKDVHYAWTSVPTYP
Sbjct: 241 ELVEMPFFQTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYP 300

Query: 301 SGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEVK 360
           SGVIGFLLCSTEGPTVDF+NPVNPIEKLGH KSKRELKFYNSEMH AAFALPSFLK EVK
Sbjct: 301 SGVIGFLLCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVK 360

Query: 361 ALSDAPASGKKGCISS 377
           ALSDA AS KKG ISS
Sbjct: 361 ALSDASASLKKGSISS 368

BLAST of Spg002488 vs. ExPASy TrEMBL
Match: A0A5D3BTV3 (Spermine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00270 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.5e-195
Identity = 340/377 (90.19%), Postives = 354/377 (93.90%), Query Frame = 0

Query: 1   MEDVTGRSL-ECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSDF 60
           MEDVTG+SL ECQK+MDGK TNGNG E  IPSCCLKAKTS P+LEAQCHSTVVSGWFS+F
Sbjct: 1   MEDVTGKSLVECQKVMDGKVTNGNGKE-VIPSCCLKAKTSDPKLEAQCHSTVVSGWFSEF 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
           QSGSD+A K++YFNNPMWP        GEAHAL+V+NILFKGKSEYQEVMVFESASYGKV
Sbjct: 61  QSGSDKACKKLYFNNPMWP--------GEAHALEVENILFKGKSEYQEVMVFESASYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPK+VLVVGGGDGGVLREISRHDSVEHIDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKSVLVVGGGDGGVLREISRHDSVEHIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEID+MVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAK GKYDAIIVDSSDPVGPA
Sbjct: 181 CEIDQMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAIIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
           QELVEMPFFR  A+ALRPGGVLCNMAESMWLHTHLIQDMI IC QTFKDVHYAWTSVPTY
Sbjct: 241 QELVEMPFFRMTAQALRPGGVLCNMAESMWLHTHLIQDMISICCQTFKDVHYAWTSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKLGHPKSKRELKFYNSEMHSAAFALPSFLKKEV 360
           PSGVIGFLLCSTEGP VDF+NPVNPIEKLGH KSKRELKFYNSEMHSAAFALPSFLK+EV
Sbjct: 301 PSGVIGFLLCSTEGPKVDFRNPVNPIEKLGHLKSKRELKFYNSEMHSAAFALPSFLKREV 360

Query: 361 KALSDAPASGKKGCISS 377
           KALSDAPASGK GCISS
Sbjct: 361 KALSDAPASGKNGCISS 368

BLAST of Spg002488 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 573.2 bits (1476), Expect = 1.6e-163
Identity = 279/364 (76.65%), Postives = 308/364 (84.62%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSD-F 60
           ME   G  L CQ  MDGKA+NGNGLEK +PSCCLKA    PE +A+CHSTVVSGWFS+  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
                + GK VYFNNPMWP        GEAH+L+V+ +LFK KS++QEV+VFESA+YGKV
Sbjct: 61  PRSGKKGGKAVYFNNPMWP--------GEAHSLKVEKVLFKDKSDFQEVLVFESATYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEIDKMVIDVSKKFFP+LAVGF+DPRV LH+GDA EFLR +  GKYDAIIVDSSDPVGPA
Sbjct: 181 CEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
             LVE PFF T+ARAL+PGGVLCNMAESMWLHTHLI+DMI ICRQTFK VHYAW+SVPTY
Sbjct: 241 LALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKL-GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           PSGVIGF+LCSTEGP VDFKNP+NPIEKL G    KRELKFYNS+MH AAFALP+FL++E
Sbjct: 301 PSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRRE 356

Query: 361 VKAL 363
           V +L
Sbjct: 361 VASL 356

BLAST of Spg002488 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 573.2 bits (1476), Expect = 1.6e-163
Identity = 279/364 (76.65%), Postives = 308/364 (84.62%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSD-F 60
           ME   G  L CQ  MDGKA+NGNGLEK +PSCCLKA    PE +A+CHSTVVSGWFS+  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
                + GK VYFNNPMWP        GEAH+L+V+ +LFK KS++QEV+VFESA+YGKV
Sbjct: 61  PRSGKKGGKAVYFNNPMWP--------GEAHSLKVEKVLFKDKSDFQEVLVFESATYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEIDKMVIDVSKKFFP+LAVGF+DPRV LH+GDA EFLR +  GKYDAIIVDSSDPVGPA
Sbjct: 181 CEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
             LVE PFF T+ARAL+PGGVLCNMAESMWLHTHLI+DMI ICRQTFK VHYAW+SVPTY
Sbjct: 241 LALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKL-GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           PSGVIGF+LCSTEGP VDFKNP+NPIEKL G    KRELKFYNS+MH AAFALP+FL++E
Sbjct: 301 PSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRRE 356

Query: 361 VKAL 363
           V +L
Sbjct: 361 VASL 356

BLAST of Spg002488 vs. TAIR 10
Match: AT5G53120.3 (spermidine synthase 3 )

HSP 1 Score: 573.2 bits (1476), Expect = 1.6e-163
Identity = 279/364 (76.65%), Postives = 308/364 (84.62%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSD-F 60
           ME   G  L CQ  MDGKA+NGNGLEK +PSCCLKA    PE +A+CHSTVVSGWFS+  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
                + GK VYFNNPMWP        GEAH+L+V+ +LFK KS++QEV+VFESA+YGKV
Sbjct: 61  PRSGKKGGKAVYFNNPMWP--------GEAHSLKVEKVLFKDKSDFQEVLVFESATYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEIDKMVIDVSKKFFP+LAVGF+DPRV LH+GDA EFLR +  GKYDAIIVDSSDPVGPA
Sbjct: 181 CEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
             LVE PFF T+ARAL+PGGVLCNMAESMWLHTHLI+DMI ICRQTFK VHYAW+SVPTY
Sbjct: 241 LALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKL-GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           PSGVIGF+LCSTEGP VDFKNP+NPIEKL G    KRELKFYNS+MH AAFALP+FL++E
Sbjct: 301 PSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRRE 356

Query: 361 VKAL 363
           V +L
Sbjct: 361 VASL 356

BLAST of Spg002488 vs. TAIR 10
Match: AT5G53120.4 (spermidine synthase 3 )

HSP 1 Score: 573.2 bits (1476), Expect = 1.6e-163
Identity = 279/364 (76.65%), Postives = 308/364 (84.62%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSD-F 60
           ME   G  L CQ  MDGKA+NGNGLEK +PSCCLKA    PE +A+CHSTVVSGWFS+  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
                + GK VYFNNPMWP        GEAH+L+V+ +LFK KS++QEV+VFESA+YGKV
Sbjct: 61  PRSGKKGGKAVYFNNPMWP--------GEAHSLKVEKVLFKDKSDFQEVLVFESATYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEIDKMVIDVSKKFFP+LAVGF+DPRV LH+GDA EFLR +  GKYDAIIVDSSDPVGPA
Sbjct: 181 CEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
             LVE PFF T+ARAL+PGGVLCNMAESMWLHTHLI+DMI ICRQTFK VHYAW+SVPTY
Sbjct: 241 LALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKL-GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           PSGVIGF+LCSTEGP VDFKNP+NPIEKL G    KRELKFYNS+MH AAFALP+FL++E
Sbjct: 301 PSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRRE 356

Query: 361 VKAL 363
           V +L
Sbjct: 361 VASL 356

BLAST of Spg002488 vs. TAIR 10
Match: AT5G53120.5 (spermidine synthase 3 )

HSP 1 Score: 573.2 bits (1476), Expect = 1.6e-163
Identity = 279/364 (76.65%), Postives = 308/364 (84.62%), Query Frame = 0

Query: 1   MEDVTGRSLECQKIMDGKATNGNGLEKAIPSCCLKAKTSGPELEAQCHSTVVSGWFSD-F 60
           ME   G  L CQ  MDGKA+NGNGLEK +PSCCLKA    PE +A+CHSTVVSGWFS+  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDEAGKEVYFNNPMWPADVIVSLSGEAHALQVKNILFKGKSEYQEVMVFESASYGKV 120
                + GK VYFNNPMWP        GEAH+L+V+ +LFK KS++QEV+VFESA+YGKV
Sbjct: 61  PRSGKKGGKAVYFNNPMWP--------GEAHSLKVEKVLFKDKSDFQEVLVFESATYGKV 120

Query: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDI 180
           LVLDGIVQLTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDI
Sbjct: 121 LVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDI 180

Query: 181 CEIDKMVIDVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKPGKYDAIIVDSSDPVGPA 240
           CEIDKMVIDVSKKFFP+LAVGF+DPRV LH+GDA EFLR +  GKYDAIIVDSSDPVGPA
Sbjct: 181 CEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPA 240

Query: 241 QELVEMPFFRTIARALRPGGVLCNMAESMWLHTHLIQDMILICRQTFKDVHYAWTSVPTY 300
             LVE PFF T+ARAL+PGGVLCNMAESMWLHTHLI+DMI ICRQTFK VHYAW+SVPTY
Sbjct: 241 LALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTY 300

Query: 301 PSGVIGFLLCSTEGPTVDFKNPVNPIEKL-GHPKSKRELKFYNSEMHSAAFALPSFLKKE 360
           PSGVIGF+LCSTEGP VDFKNP+NPIEKL G    KRELKFYNS+MH AAFALP+FL++E
Sbjct: 301 PSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRRE 356

Query: 361 VKAL 363
           V +L
Sbjct: 361 VASL 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878719.14.1e-19891.76spermine synthase-like [Benincasa hispida][more]
XP_004150312.15.4e-19890.43spermine synthase [Cucumis sativus] >KGN58277.1 hypothetical protein Csa_017516 ... [more]
XP_022141267.12.7e-19791.27spermine synthase-like [Momordica charantia][more]
XP_022944858.11.7e-19690.96spermine synthase-like [Cucurbita moschata][more]
KAG7013301.18.6e-19690.98Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q94BN22.2e-16276.65Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1[more]
O821471.0e-12267.09Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1[more]
Q9ZUB32.2e-12259.04Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1[more]
Q965572.9e-12263.84Spermidine synthase 2 OS=Datura stramonium OX=4076 PE=2 SV=1[more]
Q965565.0e-12264.76Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LC472.6e-19890.43PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G604620 PE=3 S... [more]
A0A6J1CJE61.3e-19791.27spermine synthase-like OS=Momordica charantia OX=3673 GN=LOC111011708 PE=3 SV=1[more]
A0A6J1FZ778.4e-19790.96spermine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111449258 PE=3 SV=1[more]
A0A6J1HVE72.7e-19590.43spermine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111467782 PE=3 SV=1[more]
A0A5D3BTV33.5e-19590.19Spermine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
Match NameE-valueIdentityDescription
AT5G53120.11.6e-16376.65spermidine synthase 3 [more]
AT5G53120.21.6e-16376.65spermidine synthase 3 [more]
AT5G53120.31.6e-16376.65spermidine synthase 3 [more]
AT5G53120.41.6e-16376.65spermidine synthase 3 [more]
AT5G53120.51.6e-16376.65spermidine synthase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR030668Spermidine/spermine synthase, eukaryotesPIRSFPIRSF000502Spermidine_synthcoord: 42..364
e-value: 6.4E-135
score: 446.3
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 46..127
e-value: 3.2E-25
score: 90.3
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 77..130
e-value: 3.0E-19
score: 68.7
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 133..320
e-value: 5.9E-65
score: 218.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 128..363
e-value: 6.1E-95
score: 319.1
NoneNo IPR availablePANTHERPTHR11558:SF25SPERMINE SYNTHASEcoord: 1..368
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 175..261
e-value: 3.55737E-10
score: 54.7434
IPR001045Spermidine/spermine synthasesTIGRFAMTIGR00417TIGR00417coord: 87..354
e-value: 4.2E-93
score: 309.5
IPR001045Spermidine/spermine synthasesPANTHERPTHR11558SPERMIDINE/SPERMINE SYNTHASEcoord: 1..368
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 54..360
score: 34.613232
IPR030373Polyamine biosynthesis domain, conserved sitePROSITEPS01330PABS_1coord: 154..167
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 80..311
score: 92.860977
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 84..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002488.1Spg002488.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0006596 polyamine biosynthetic process
biological_process GO:0006595 polyamine metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0004766 spermidine synthase activity
molecular_function GO:0003824 catalytic activity