Spg002478 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002478
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	DUF4283 domain-containing protein
Location	scaffold6: 3307865 .. 3309971 (+)
RNA-Seq Expression	Spg002478
Synteny	Spg002478

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAACTGGCATGATATCATGCGAGCATTACAGCAGAACCTCAGCCTTCTCCTCAGTTAGCCCCCTTCAGCCAGACAAAGCCCTCCTTGCTTGTGAGGATGAAGAACAAGCTCGGACCTTAGCCAATATCAAAGGATGGTACAAGGTTGGAAAATACCAAGTCCATTTCTTCCCATGGAGTGCGGAAACTATGCTAGGCAAGCCAAAGGTTCCGTCGTATGGTGGAAGGATCAAAGTCAGAAACCTTCCCCTAGACAAATGGTCCATAGAAACTTTCAAGGAAATCGGAGATAAATGTGGGGGATACTTGGAAACTGCCAGCAAAACTCTATCTCGTATGGACATGATGGAGATAGGCATTAAGGTTAAAGAAAATAGCATGGGCTTCATCCCAACAGAAGTCCACATCCCTTTGTCCTCTTGCAACCCCATAGTGGTTAAAATCGACCCCTTTTTTGTTGAAGACTTCAACATAGGCTACATAGCCGGTATCCATGGCAGAATTCCAGCTCCTCTAGGAGAAAACAACAATCCACACGCCGTCGAAGATAAAGAAAATGACTATAGGGAAGAAAAGGACAATCCACGCGCCGCTGCTATAGTGGTGGAGGACAGCAAGAAAGAAAATCCCCATAGCTCATATAGGCGAGAAACCCAAAGCGCCCCATCAATGACTTGTTCACATCATCTGATCTGATCCCTGTCGAACTACCAGGTGCAGGCCCACATATATCTACATCTTCTAAGGAGCCCCAATCCCCAAAATCCACTCCCACAAGCCGAGAAAAGACCCACCCATTAGAAGCCCCCAATAAAGCCAAGCCCTTTGACCGAAGCTCGAAATCCACATCTGCATACCGACAAAATTCTCCCACCCATCAACCTCCTTTTAGACCTGCACAAAAAAGCCCAGACCCACCAAATGATACCCACCATGACACGAAAAAGCCCATCATTATTAACAATAAAGAAACCTTCCTTATCACGAGAATCATGCATTCCACCAATAATGATCTTCCCATATCAGATTCGGGAAGGAGTGTTGTCATCTCCCTGCACTACCGCTATGGAGGATTCCTCGTTACCGACCCCCTATAGAGCTATTATACAAACAGCTTCCCCACCAACCATTTGCAAACTTTTCGAGTCACAACATGAACAACAACCATATATCGAGGACCCCATCCCACTGAGTATTGAAGAACCAAATCCTCTAAAAGCTCAGCTAGCTATGGGTATAGAGGAAACAACTCTCATTGATATAGAAGTAGAAAAAGCGGATACAGAGGACATGGAAACAAGGGAAAATTGTGTGGACCCAGCCTTATATTTACCCATATTATTTCCATGGTTGACCGAACATGGTATGTGCATTATGCCAATGCCAAGCAGACACAAAACCACGAATTCCACAAAGAAGAAGACAAAATGGGTTAAAGAACTACCGAATTTGCACTCCACAGTTAATTATGAGAAGGCTCTAACAGGTTGCCTTAAGGACAGGTCGAGAACTTCCTCATGATTTTTATCTCTTGGAATGTTCGTGGACTAGGTTCTTGGGAAAAAAAAAGAAAAAAAAGAAAAGAAAAGAAAAAAGAGCCCTTATTAAGGATTATCTCACTTCCCAAAACCCATCATTGGTGATTTTTCAAGAAACAAAGCTTGCCAACATTGACGGAAAATTTATCAAATCTATTTGGAGCTTTAGGAACATTGCTTGGACCTCCATTGATACGGTTGGTGCTTCTGGTGGCATTGCTATCATATGGAATGATTACTCTTTCACTGTGTTGGAAGTTATTGAAGGTATATTCTCGTTATCCATCCACCTCTCTCTTGTTGATGGGTTCTCCTTTTGGATTATAGGGGTTTATGACCCAAATTCTTCTCGGGACAAGAAAGTATTCTGGAGAGAGTTGGCCGACCTTCAAGCTCTTTGTCTACCCAACTGGGTATTGGGAGATGATTTTAACGTTACCCGTTGGACTTGGGAGAAGTCTACCATCACAGCTCCTTCCCGAGTCATGAGGACATTTAACCGCTTTATTGAATCAGCTGCTTTACAGGACCATCCCCCTCTCCAATGGCAAATTTACTTGGTTTAG

mRNA sequence

ATGATAACTGGCATGATATCATGCGAGCATTACAGCAGAACCTCAGCCTTCTCCTCAGTTAGCCCCCTTCAGCCAGACAAAGCCCTCCTTGCTTGTGAGGATGAAGAACAAGCTCGGACCTTAGCCAATATCAAAGGATGGTACAAGGTTGGAAAATACCAAGTCCATTTCTTCCCATGGAGTGCGGAAACTATGCTAGGCAAGCCAAAGGTTCCGTCGTATGGTGGAAGGATCAAAGTCAGAAACCTTCCCCTAGACAAATGGTCCATAGAAACTTTCAAGGAAATCGGAGATAAATGTGGGGGATACTTGGAAACTGCCAGCAAAACTCTATCTCGTATGGACATGATGGAGATAGGCATTAAGGTTAAAGAAAATAGCATGGGCTTCATCCCAACAGAAGTCCACATCCCTTTGTCCTCTTGCAACCCCATAGTGGTTAAAATCGACCCCTTTTTTGTTGAAGACTTCAACATAGGCTACATAGCCGGTATCCATGGCAGAATTCCAGCTCCTCTAGGAGAAAACAACAATCCACACGCCGTCGAAGATAAAGAAAATGACTATAGGGAAGAAAAGGACAATCCACGCGCCGCTGCTATAATTCGGGAAGGAGTGTTGTCATCTCCCTGCACTACCGCTATGGAGGATTCCTCGTTACCGACCCCCTATAGAGCTATTATACAAACAGCTTCCCCACCAACCATTTGCAAACTTTTCGAGTCACAACATGAACAACAACCATATATCGAGGACCCCATCCCACTGAGTATTGAAGAACCAAATCCTCTAAAAGCTCAGCTAGCTATGGGTATAGAGGAAACAACTCTCATTGATATAGAAGTAGAAAAAGCGGATACAGAGGACATGGAAACAAGGGAAAATTGTGTGGACCCAGCCTTATATTTACCCATATTATTTCCATGGTTGACCGAACATGGTATGTGCATTATGCCAATGCCAAGCAGACACAAAACCACGAATTCCACAAAGAAGAAGACAAAATGGGTTAAAGAACTACCGAATTTGCACTCCACAGTTAATTATGAGAAGGCTCTAACAGGTTGCCTTAAGGACAGGAACATTGCTTGGACCTCCATTGATACGGTTGGTGCTTCTGGTGGCATTGCTATCATATGGAATGATTACTCTTTCACTGTGTTGGAAGTTATTGAAGGTATATTCTCGTTATCCATCCACCTCTCTCTTGTTGATGGGTTCTCCTTTTGGATTATAGGGGTTTATGACCCAAATTCTTCTCGGGACAAGAAAGTATTCTGGAGAGAGTTGGCCGACCTTCAAGCTCTTTGTCTACCCAACTGGGTATTGGGAGATGATTTTAACGTTACCCGTTGGACTTGGGAGAAGTCTACCATCACAGCTCCTTCCCGAGTCATGAGGACATTTAACCGCTTTATTGAATCAGCTGCTTTACAGGACCATCCCCCTCTCCAATGGCAAATTTACTTGGTTTAG

Coding sequence (CDS)

ATGATAACTGGCATGATATCATGCGAGCATTACAGCAGAACCTCAGCCTTCTCCTCAGTTAGCCCCCTTCAGCCAGACAAAGCCCTCCTTGCTTGTGAGGATGAAGAACAAGCTCGGACCTTAGCCAATATCAAAGGATGGTACAAGGTTGGAAAATACCAAGTCCATTTCTTCCCATGGAGTGCGGAAACTATGCTAGGCAAGCCAAAGGTTCCGTCGTATGGTGGAAGGATCAAAGTCAGAAACCTTCCCCTAGACAAATGGTCCATAGAAACTTTCAAGGAAATCGGAGATAAATGTGGGGGATACTTGGAAACTGCCAGCAAAACTCTATCTCGTATGGACATGATGGAGATAGGCATTAAGGTTAAAGAAAATAGCATGGGCTTCATCCCAACAGAAGTCCACATCCCTTTGTCCTCTTGCAACCCCATAGTGGTTAAAATCGACCCCTTTTTTGTTGAAGACTTCAACATAGGCTACATAGCCGGTATCCATGGCAGAATTCCAGCTCCTCTAGGAGAAAACAACAATCCACACGCCGTCGAAGATAAAGAAAATGACTATAGGGAAGAAAAGGACAATCCACGCGCCGCTGCTATAATTCGGGAAGGAGTGTTGTCATCTCCCTGCACTACCGCTATGGAGGATTCCTCGTTACCGACCCCCTATAGAGCTATTATACAAACAGCTTCCCCACCAACCATTTGCAAACTTTTCGAGTCACAACATGAACAACAACCATATATCGAGGACCCCATCCCACTGAGTATTGAAGAACCAAATCCTCTAAAAGCTCAGCTAGCTATGGGTATAGAGGAAACAACTCTCATTGATATAGAAGTAGAAAAAGCGGATACAGAGGACATGGAAACAAGGGAAAATTGTGTGGACCCAGCCTTATATTTACCCATATTATTTCCATGGTTGACCGAACATGGTATGTGCATTATGCCAATGCCAAGCAGACACAAAACCACGAATTCCACAAAGAAGAAGACAAAATGGGTTAAAGAACTACCGAATTTGCACTCCACAGTTAATTATGAGAAGGCTCTAACAGGTTGCCTTAAGGACAGGAACATTGCTTGGACCTCCATTGATACGGTTGGTGCTTCTGGTGGCATTGCTATCATATGGAATGATTACTCTTTCACTGTGTTGGAAGTTATTGAAGGTATATTCTCGTTATCCATCCACCTCTCTCTTGTTGATGGGTTCTCCTTTTGGATTATAGGGGTTTATGACCCAAATTCTTCTCGGGACAAGAAAGTATTCTGGAGAGAGTTGGCCGACCTTCAAGCTCTTTGTCTACCCAACTGGGTATTGGGAGATGATTTTAACGTTACCCGTTGGACTTGGGAGAAGTCTACCATCACAGCTCCTTCCCGAGTCATGAGGACATTTAACCGCTTTATTGAATCAGCTGCTTTACAGGACCATCCCCCTCTCCAATGGCAAATTTACTTGGTTTAG

Protein sequence

MITGMISCEHYSRTSAFSSVSPLQPDKALLACEDEEQARTLANIKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGGRIKVRNLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVHIPLSSCNPIVVKIDPFFVEDFNIGYIAGIHGRIPAPLGENNNPHAVEDKENDYREEKDNPRAAAIIREGVLSSPCTTAMEDSSLPTPYRAIIQTASPPTICKLFESQHEQQPYIEDPIPLSIEEPNPLKAQLAMGIEETTLIDIEVEKADTEDMETRENCVDPALYLPILFPWLTEHGMCIMPMPSRHKTTNSTKKKTKWVKELPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDYSFTVLEVIEGIFSLSIHLSLVDGFSFWIIGVYDPNSSRDKKVFWRELADLQALCLPNWVLGDDFNVTRWTWEKSTITAPSRVMRTFNRFIESAALQDHPPLQWQIYLV

Homology

[Close]

BLAST of Spg002478 vs. NCBI nr
Match: KAA0044449.1 (hypothetical protein E6C27_scaffold46G001820 [Cucumis melo var. makuwa])

HSP 1 Score: 149.1 bits (375), Expect = 1.0e-31
Identity = 140/570 (24.56%), Postives = 222/570 (38.95%), Query Frame = 0


Query: 22  PLQPDKALLACEDEEQARTLANIKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGGRIKVR 81
           P   DKALL  +D+E A+ L    GW  VG + V F  WS         +PSYGG  + R
Sbjct: 226 PFHADKALLFIKDKELAKLLCKNIGWTTVGPFYVKFEKWSKNAHADTKVIPSYGGWTRFR 285

Query: 82  NLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVHIPLSS 141
            +PL  W++ TF +IG+  GG+++ A ++++++++ E  IKVKEN  GF+P  + I    
Sbjct: 286 GIPLHIWNLNTFIQIGEAYGGFIDAAPESVNKLELTEALIKVKENYTGFLPAFIQIHDEE 345

Query: 142 CNPIVVKIDPFFVEDFNIGYIAGIHGRIPAPLGEN---NNPHAVE--------------- 201
            +  +++        +       IHG       EN    NP+A +               
Sbjct: 346 GHDFIIQTVTHPKGKWLRERNPSIHGSFTKTAAENFNEFNPYAEQYTFRRNLAVIAKPDL 405

Query: 202 ---------DKEND----------------------YREEKDNPRAAAIIREGVLSSPCT 261
                       ND                      Y  + DN  ++  I++    S  T
Sbjct: 406 PESSKKGSKQMNNDRKMGPTKTIKKFNKTVSFLNLNYEGDSDNSNSSEKIQKKDHRSEMT 465

Query: 262 TAMEDSSL----------PTPYRAIIQTASPPTICKLFESQHEQQPYIEDPIPLSIEEPN 321
              +   +          P   +  +  +SP     LF +       ++   P+S  +  
Sbjct: 466 EKKKGKQICCGEYNQKISPINTKRKVSFSSPKNETFLFSAHSAPTKTLKLGSPMSYAKME 525

Query: 322 PLKAQLAMGIE-------------ETTLIDIEVEKADTEDME---------------TRE 381
               +    I+             ET+    + +K   +  E               T  
Sbjct: 526 AKSKRPTQSIKKKVYRVKSRSMERETSQTSRQKDKGKIDPNEFELVVDLGHISLLSDTDF 585

Query: 382 NCVDPALYLP---------ILFPWLTEHGMCIMPMPSRHKTTN----------STKKK-T 441
           +C +   Y+P         I+   L     C      + K  N          S K+K T
Sbjct: 586 SCPESPSYIPSPTSPTESDIVKDSLASMMTCAHEDREKKKKENIREETEDDEVSFKRKLT 645

Query: 442 KWVKELPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDYSFTVLEVIEGI 485
            W+KE  NL    ++         DR I   SI     + G+  +  D       VI+G 
Sbjct: 646 DWLKE-NNLRLAADFNSQFNSVTNDRMI---SILNGPPNVGVENVSED-------VIDGA 705

[Close]

BLAST of Spg002478 vs. NCBI nr
Match: TYK08190.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 142.9 bits (359), Expect = 7.4e-30
Identity = 136/581 (23.41%), Postives = 231/581 (39.76%), Query Frame = 0


Query: 19  SVSPLQPDKALLACEDEEQARTLAN--IKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGG 78
           S  P Q DKA+L    +      +N    GW  VG YQV F  W +        +PSYGG
Sbjct: 210 SYKPFQADKAILFLNPDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFHSVIPSYGG 269

Query: 79  RIKVRNLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVH 138
            ++ R +PL  W+  TF+ IG  CGG+L+ A +T+    +++  IKV+ N +GF+P  + 
Sbjct: 270 WLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYIGFVPASIL 329

Query: 139 IPLSSC-NPIVVKIDPF---FVEDFNI-----------------GYIA------GIHGRI 198
           I  +   N IV  + P    ++ + N+                  ++A      G     
Sbjct: 330 ITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYTYNGFQAIP 389

Query: 199 PAPLG-------ENNNPHAV----EDKENDYREEKDNP-------------RAAAIIRE- 258
           P P          N++ H++    + K+N+  E + +P             +A  II + 
Sbjct: 390 PEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGKAILIINDQ 449

Query: 259 -----------------------GVLSSPCTT-------AMEDSSLPTPYRAIIQTASPP 318
                                  G+ S+   T       ++E S++   +          
Sbjct: 450 DHGHYSKRSKRISNRKVSFLSPGGIQSNSSNTEINTKGKSLEISTINDQFEKRWSPRQKT 509

Query: 319 TICKLFESQHEQQPYIEDPIPLSIEEPNPLKAQLAMGIEETTLIDIE--VEKADTEDMET 378
                +  + + Q   ED   LS++E      Q+ + ++   +  +E  ++  +   ++T
Sbjct: 510 KTKLTYRIKKDPQESTEDH-KLSLKETGEGSKQMNLSVDMGPISPLESMIQSENNHGLDT 569

Query: 379 REN--------CVDPALYLPILFPWLTEHGMCIMPMPSRHKTTNSTK------------- 438
             N          D A    +      + G       SR     ++K             
Sbjct: 570 FNNQTPDGNSKSTDSAEAKNLTVS--VKEGADQNKSASRSTAEGNSKDAKTGSELEIDRA 629

Query: 439 ---KKTKWVKE-----LPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDY 485
              K   W+KE      P   + V         + D+N+       +G  GGI ++W+D 
Sbjct: 630 FKEKLVIWLKENELKLSPKYTNDVPSSSYFPVIVSDQNMDIAGHGPLGDKGGILVLWDDT 689

[Close]

BLAST of Spg002478 vs. NCBI nr
Match: KAA0057507.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 142.9 bits (359), Expect = 7.4e-30
Identity = 136/581 (23.41%), Postives = 231/581 (39.76%), Query Frame = 0


Query: 19  SVSPLQPDKALLACEDEEQARTLAN--IKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGG 78
           S  P Q DKA+L    +      +N    GW  VG YQV F  W +        +PSYGG
Sbjct: 210 SYKPFQADKAILFLNPDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFHSVIPSYGG 269

Query: 79  RIKVRNLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVH 138
            ++ R +PL  W+  TF+ IG  CGG+L+ A +T+    +++  IKV+ N +GF+P  + 
Sbjct: 270 WLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYIGFVPASIL 329

Query: 139 IPLSSC-NPIVVKIDPF---FVEDFNI-----------------GYIA------GIHGRI 198
           I  +   N IV  + P    ++ + N+                  ++A      G     
Sbjct: 330 ITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYTYNGFQAIP 389

Query: 199 PAPLG-------ENNNPHAV----EDKENDYREEKDNP-------------RAAAIIRE- 258
           P P          N++ H++    + K+N+  E + +P             +A  II + 
Sbjct: 390 PEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGKAILIINDQ 449

Query: 259 -----------------------GVLSSPCTT-------AMEDSSLPTPYRAIIQTASPP 318
                                  G+ S+   T       ++E S++   +          
Sbjct: 450 DHGHYSKRSKRISNRKVSFLSPGGIQSNSSNTEINTKGKSLEISTINDQFEKRWSPRQKT 509

Query: 319 TICKLFESQHEQQPYIEDPIPLSIEEPNPLKAQLAMGIEETTLIDIE--VEKADTEDMET 378
                +  + + Q   ED   LS++E      Q+ + ++   +  +E  ++  +   ++T
Sbjct: 510 KTKLTYRIKKDPQESTEDH-KLSLKETGEGSKQMNLSVDMGPISPLESMIQSENNHGLDT 569

Query: 379 REN--------CVDPALYLPILFPWLTEHGMCIMPMPSRHKTTNSTK------------- 438
             N          D A    +      + G       SR     ++K             
Sbjct: 570 FNNQTPDGNSKSTDSAEAKNLTVS--VKEGADQNKSASRSTAEGNSKDAKTGSELEIDRA 629

Query: 439 ---KKTKWVKE-----LPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDY 485
              K   W+KE      P   + V         + D+N+       +G  GGI ++W+D 
Sbjct: 630 FKEKLVIWLKENELKLSPKYTNDVPSSSYFPVIVSDQNMDIAGHGPLGDKGGILVLWDDT 689

[Close]

BLAST of Spg002478 vs. NCBI nr
Match: KAA0056838.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYJ99341.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 139.4 bits (350), Expect = 8.1e-29
Identity = 136/581 (23.41%), Postives = 230/581 (39.59%), Query Frame = 0


Query: 19  SVSPLQPDKALLACEDEEQARTLAN--IKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGG 78
           S  P Q DKA+L    +      +N    GW  VG YQV F  W +        +PSYGG
Sbjct: 210 SYKPFQADKAILFLNSDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFHSVIPSYGG 269

Query: 79  RIKVRNLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVH 138
            ++ R +PL  W+  TF+ IG  CGG+L+ A +T+    +++  IKV+ N  GF+P  + 
Sbjct: 270 WLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYTGFVPASIL 329

Query: 139 IPLSSC-NPIVVKIDPF---FVEDFNI-----------------GYIA------GIHGRI 198
           I  +   N IV  + P    ++ + N+                  ++A      G     
Sbjct: 330 ITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYTYNGFQAIP 389

Query: 199 PAPLG-------ENNNPHAV----EDKENDYREEKDNP-------------RAAAIIRE- 258
           P P          N++ H++    + K+N+  E + +P             +A  II + 
Sbjct: 390 PEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGKAILIINDQ 449

Query: 259 -----------------------GVLSSPCTT-------AMEDSSLPTPYRAIIQTASPP 318
                                  G+ S+   T       ++E S++   +          
Sbjct: 450 NHGHYSKRSKRISNRKVSFLSPGGIQSNSSNTEINTKGKSLEISTINDQFEKRWSPRQKT 509

Query: 319 TICKLFESQHEQQPYIEDPIPLSIEEPNPLKAQLAMGIEETTLIDIE--VEKADTEDMET 378
                +  + + Q   ED   LS++E      Q+ + ++   +  +E  ++  +   ++T
Sbjct: 510 KTKLTYRIKKDPQESTEDH-NLSLKETGEGSKQMNLSVDMGPISPLESMIQSENNHGLDT 569

Query: 379 REN--------CVDPALYLPILFPWLTEHGMCIMPMPSRHKTTNSTK------------- 438
             N          D A    +      + G       SR     ++K             
Sbjct: 570 LNNQTPDGNSKSTDSAEAKNLTVS--VKEGADQNKSASRSTAEGNSKDAKTGSEMEIDRA 629

Query: 439 ---KKTKWVKE-----LPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDY 485
              K   W+KE      P   + V    +    + D+N+       +G  GGI ++W+D 
Sbjct: 630 FKEKLVIWLKENELKLSPKYTNDVPSSSSFPVIVSDQNMDIAGHGPLGDKGGILVLWDDT 689

[Close]

BLAST of Spg002478 vs. NCBI nr
Match: XP_022145142.1 (uncharacterized protein LOC111014657 [Momordica charantia])

HSP 1 Score: 134.4 bits (337), Expect = 2.6e-27
Identity = 58/120 (48.33%), Postives = 80/120 (66.67%), Query Frame = 0


Query: 361 NIAWTSIDTVGASGGIAIIWNDYSFTVLEVIEGIFSLSIHLSLVDGFSFWIIGVYDPNSS 420
           +IAW S+D  GASGGI ++W+  S + +EVI G FS+S+H  L D F++W+ GVY P   
Sbjct: 55  SIAWASLDASGASGGIILLWDQLSTSAVEVICGHFSISVHFKLADNFTWWLTGVYSPVKQ 114

Query: 421 RDKKVFWRELADLQALCLPNWVLGDDFNVTRWTWEKSTITAPSRVMRTFNRFIESAALQD 480
           + +K+FW+EL DL  LC P W+LG+DFN+ RW+ E S+   P   M  FN FI+ A L D
Sbjct: 115 KKRKLFWQELFDLNGLCGPIWLLGEDFNIYRWSHETSSANPPQTGMNKFNHFIDFAGLID 174

[Close]

BLAST of Spg002478 vs. ExPASy TrEMBL
Match: A0A5A7TTA1 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001820 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 5.0e-32
Identity = 140/570 (24.56%), Postives = 222/570 (38.95%), Query Frame = 0


Query: 22  PLQPDKALLACEDEEQARTLANIKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGGRIKVR 81
           P   DKALL  +D+E A+ L    GW  VG + V F  WS         +PSYGG  + R
Sbjct: 226 PFHADKALLFIKDKELAKLLCKNIGWTTVGPFYVKFEKWSKNAHADTKVIPSYGGWTRFR 285

Query: 82  NLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVHIPLSS 141
            +PL  W++ TF +IG+  GG+++ A ++++++++ E  IKVKEN  GF+P  + I    
Sbjct: 286 GIPLHIWNLNTFIQIGEAYGGFIDAAPESVNKLELTEALIKVKENYTGFLPAFIQIHDEE 345

Query: 142 CNPIVVKIDPFFVEDFNIGYIAGIHGRIPAPLGEN---NNPHAVE--------------- 201
            +  +++        +       IHG       EN    NP+A +               
Sbjct: 346 GHDFIIQTVTHPKGKWLRERNPSIHGSFTKTAAENFNEFNPYAEQYTFRRNLAVIAKPDL 405

Query: 202 ---------DKEND----------------------YREEKDNPRAAAIIREGVLSSPCT 261
                       ND                      Y  + DN  ++  I++    S  T
Sbjct: 406 PESSKKGSKQMNNDRKMGPTKTIKKFNKTVSFLNLNYEGDSDNSNSSEKIQKKDHRSEMT 465

Query: 262 TAMEDSSL----------PTPYRAIIQTASPPTICKLFESQHEQQPYIEDPIPLSIEEPN 321
              +   +          P   +  +  +SP     LF +       ++   P+S  +  
Sbjct: 466 EKKKGKQICCGEYNQKISPINTKRKVSFSSPKNETFLFSAHSAPTKTLKLGSPMSYAKME 525

Query: 322 PLKAQLAMGIE-------------ETTLIDIEVEKADTEDME---------------TRE 381
               +    I+             ET+    + +K   +  E               T  
Sbjct: 526 AKSKRPTQSIKKKVYRVKSRSMERETSQTSRQKDKGKIDPNEFELVVDLGHISLLSDTDF 585

Query: 382 NCVDPALYLP---------ILFPWLTEHGMCIMPMPSRHKTTN----------STKKK-T 441
           +C +   Y+P         I+   L     C      + K  N          S K+K T
Sbjct: 586 SCPESPSYIPSPTSPTESDIVKDSLASMMTCAHEDREKKKKENIREETEDDEVSFKRKLT 645

Query: 442 KWVKELPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDYSFTVLEVIEGI 485
            W+KE  NL    ++         DR I   SI     + G+  +  D       VI+G 
Sbjct: 646 DWLKE-NNLRLAADFNSQFNSVTNDRMI---SILNGPPNVGVENVSED-------VIDGA 705

[Close]

BLAST of Spg002478 vs. ExPASy TrEMBL
Match: A0A5A7US62 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G003960 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.6e-30
Identity = 136/581 (23.41%), Postives = 231/581 (39.76%), Query Frame = 0


Query: 19  SVSPLQPDKALLACEDEEQARTLAN--IKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGG 78
           S  P Q DKA+L    +      +N    GW  VG YQV F  W +        +PSYGG
Sbjct: 210 SYKPFQADKAILFLNPDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFHSVIPSYGG 269

Query: 79  RIKVRNLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVH 138
            ++ R +PL  W+  TF+ IG  CGG+L+ A +T+    +++  IKV+ N +GF+P  + 
Sbjct: 270 WLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYIGFVPASIL 329

Query: 139 IPLSSC-NPIVVKIDPF---FVEDFNI-----------------GYIA------GIHGRI 198
           I  +   N IV  + P    ++ + N+                  ++A      G     
Sbjct: 330 ITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYTYNGFQAIP 389

Query: 199 PAPLG-------ENNNPHAV----EDKENDYREEKDNP-------------RAAAIIRE- 258
           P P          N++ H++    + K+N+  E + +P             +A  II + 
Sbjct: 390 PEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGKAILIINDQ 449

Query: 259 -----------------------GVLSSPCTT-------AMEDSSLPTPYRAIIQTASPP 318
                                  G+ S+   T       ++E S++   +          
Sbjct: 450 DHGHYSKRSKRISNRKVSFLSPGGIQSNSSNTEINTKGKSLEISTINDQFEKRWSPRQKT 509

Query: 319 TICKLFESQHEQQPYIEDPIPLSIEEPNPLKAQLAMGIEETTLIDIE--VEKADTEDMET 378
                +  + + Q   ED   LS++E      Q+ + ++   +  +E  ++  +   ++T
Sbjct: 510 KTKLTYRIKKDPQESTEDH-KLSLKETGEGSKQMNLSVDMGPISPLESMIQSENNHGLDT 569

Query: 379 REN--------CVDPALYLPILFPWLTEHGMCIMPMPSRHKTTNSTK------------- 438
             N          D A    +      + G       SR     ++K             
Sbjct: 570 FNNQTPDGNSKSTDSAEAKNLTVS--VKEGADQNKSASRSTAEGNSKDAKTGSELEIDRA 629

Query: 439 ---KKTKWVKE-----LPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDY 485
              K   W+KE      P   + V         + D+N+       +G  GGI ++W+D 
Sbjct: 630 FKEKLVIWLKENELKLSPKYTNDVPSSSYFPVIVSDQNMDIAGHGPLGDKGGILVLWDDT 689

[Close]

BLAST of Spg002478 vs. ExPASy TrEMBL
Match: A0A5D3CA17 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00050 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.6e-30
Identity = 136/581 (23.41%), Postives = 231/581 (39.76%), Query Frame = 0


Query: 19  SVSPLQPDKALLACEDEEQARTLAN--IKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGG 78
           S  P Q DKA+L    +      +N    GW  VG YQV F  W +        +PSYGG
Sbjct: 210 SYKPFQADKAILFLNPDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFHSVIPSYGG 269

Query: 79  RIKVRNLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVH 138
            ++ R +PL  W+  TF+ IG  CGG+L+ A +T+    +++  IKV+ N +GF+P  + 
Sbjct: 270 WLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYIGFVPASIL 329

Query: 139 IPLSSC-NPIVVKIDPF---FVEDFNI-----------------GYIA------GIHGRI 198
           I  +   N IV  + P    ++ + N+                  ++A      G     
Sbjct: 330 ITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYTYNGFQAIP 389

Query: 199 PAPLG-------ENNNPHAV----EDKENDYREEKDNP-------------RAAAIIRE- 258
           P P          N++ H++    + K+N+  E + +P             +A  II + 
Sbjct: 390 PEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGKAILIINDQ 449

Query: 259 -----------------------GVLSSPCTT-------AMEDSSLPTPYRAIIQTASPP 318
                                  G+ S+   T       ++E S++   +          
Sbjct: 450 DHGHYSKRSKRISNRKVSFLSPGGIQSNSSNTEINTKGKSLEISTINDQFEKRWSPRQKT 509

Query: 319 TICKLFESQHEQQPYIEDPIPLSIEEPNPLKAQLAMGIEETTLIDIE--VEKADTEDMET 378
                +  + + Q   ED   LS++E      Q+ + ++   +  +E  ++  +   ++T
Sbjct: 510 KTKLTYRIKKDPQESTEDH-KLSLKETGEGSKQMNLSVDMGPISPLESMIQSENNHGLDT 569

Query: 379 REN--------CVDPALYLPILFPWLTEHGMCIMPMPSRHKTTNSTK------------- 438
             N          D A    +      + G       SR     ++K             
Sbjct: 570 FNNQTPDGNSKSTDSAEAKNLTVS--VKEGADQNKSASRSTAEGNSKDAKTGSELEIDRA 629

Query: 439 ---KKTKWVKE-----LPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDY 485
              K   W+KE      P   + V         + D+N+       +G  GGI ++W+D 
Sbjct: 630 FKEKLVIWLKENELKLSPKYTNDVPSSSYFPVIVSDQNMDIAGHGPLGDKGGILVLWDDT 689

[Close]

BLAST of Spg002478 vs. ExPASy TrEMBL
Match: A0A5D3BKT8 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005570 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.9e-29
Identity = 136/581 (23.41%), Postives = 230/581 (39.59%), Query Frame = 0


Query: 19  SVSPLQPDKALLACEDEEQARTLAN--IKGWYKVGKYQVHFFPWSAETMLGKPKVPSYGG 78
           S  P Q DKA+L    +      +N    GW  VG YQV F  W +        +PSYGG
Sbjct: 210 SYKPFQADKAILFLNSDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFHSVIPSYGG 269

Query: 79  RIKVRNLPLDKWSIETFKEIGDKCGGYLETASKTLSRMDMMEIGIKVKENSMGFIPTEVH 138
            ++ R +PL  W+  TF+ IG  CGG+L+ A +T+    +++  IKV+ N  GF+P  + 
Sbjct: 270 WLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYTGFVPASIL 329

Query: 139 IPLSSC-NPIVVKIDPF---FVEDFNI-----------------GYIA------GIHGRI 198
           I  +   N IV  + P    ++ + N+                  ++A      G     
Sbjct: 330 ITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYTYNGFQAIP 389

Query: 199 PAPLG-------ENNNPHAV----EDKENDYREEKDNP-------------RAAAIIRE- 258
           P P          N++ H++    + K+N+  E + +P             +A  II + 
Sbjct: 390 PEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGKAILIINDQ 449

Query: 259 -----------------------GVLSSPCTT-------AMEDSSLPTPYRAIIQTASPP 318
                                  G+ S+   T       ++E S++   +          
Sbjct: 450 NHGHYSKRSKRISNRKVSFLSPGGIQSNSSNTEINTKGKSLEISTINDQFEKRWSPRQKT 509

Query: 319 TICKLFESQHEQQPYIEDPIPLSIEEPNPLKAQLAMGIEETTLIDIE--VEKADTEDMET 378
                +  + + Q   ED   LS++E      Q+ + ++   +  +E  ++  +   ++T
Sbjct: 510 KTKLTYRIKKDPQESTEDH-NLSLKETGEGSKQMNLSVDMGPISPLESMIQSENNHGLDT 569

Query: 379 REN--------CVDPALYLPILFPWLTEHGMCIMPMPSRHKTTNSTK------------- 438
             N          D A    +      + G       SR     ++K             
Sbjct: 570 LNNQTPDGNSKSTDSAEAKNLTVS--VKEGADQNKSASRSTAEGNSKDAKTGSEMEIDRA 629

Query: 439 ---KKTKWVKE-----LPNLHSTVNYEKALTGCLKDRNIAWTSIDTVGASGGIAIIWNDY 485
              K   W+KE      P   + V    +    + D+N+       +G  GGI ++W+D 
Sbjct: 630 FKEKLVIWLKENELKLSPKYTNDVPSSSSFPVIVSDQNMDIAGHGPLGDKGGILVLWDDT 689

[Close]

BLAST of Spg002478 vs. ExPASy TrEMBL
Match: A0A6J1CVN2 (uncharacterized protein LOC111014657 OS=Momordica charantia OX=3673 GN=LOC111014657 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-27
Identity = 58/120 (48.33%), Postives = 80/120 (66.67%), Query Frame = 0


Query: 361 NIAWTSIDTVGASGGIAIIWNDYSFTVLEVIEGIFSLSIHLSLVDGFSFWIIGVYDPNSS 420
           +IAW S+D  GASGGI ++W+  S + +EVI G FS+S+H  L D F++W+ GVY P   
Sbjct: 55  SIAWASLDASGASGGIILLWDQLSTSAVEVICGHFSISVHFKLADNFTWWLTGVYSPVKQ 114

Query: 421 RDKKVFWRELADLQALCLPNWVLGDDFNVTRWTWEKSTITAPSRVMRTFNRFIESAALQD 480
           + +K+FW+EL DL  LC P W+LG+DFN+ RW+ E S+   P   M  FN FI+ A L D
Sbjct: 115 KKRKLFWQELFDLNGLCGPIWLLGEDFNIYRWSHETSSANPPQTGMNKFNHFIDFAGLID 174

The following BLAST results are available for this feature:

BLAST of Spg002478 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0044449.1	1.0e-31	24.56	hypothetical protein E6C27_scaffold46G001820 [Cucumis melo var. makuwa]	[more]
TYK08190.1	7.4e-30	23.41	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
KAA0057507.1	7.4e-30	23.41	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
KAA0056838.1	8.1e-29	23.41	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYJ993...	[more]
XP_022145142.1	2.6e-27	48.33	uncharacterized protein LOC111014657 [Momordica charantia]	[more]

BLAST of Spg002478 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg002478 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TTA1	5.0e-32	24.56	DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5A7US62	3.6e-30	23.41	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5D3CA17	3.6e-30	23.41	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5D3BKT8	3.9e-29	23.41	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1CVN2	1.3e-27	48.33	uncharacterized protein LOC111014657 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]

BLAST of Spg002478 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	GENE3D	3.60.10.10	Endonuclease/exonuclease/phosphatase	coord: 327..486 e-value: 2.2E-10 score: 42.7
IPR036691	Endonuclease/exonuclease/phosphatase superfamily	SUPERFAMILY	56219	DNase I-like	coord: 351..481
IPR025558	Domain of unknown function DUF4283	PFAM	PF14111	DUF4283	coord: 28..117 e-value: 4.0E-9 score: 36.2
IPR004808	AP endonuclease 1	PANTHER	PTHR22748	AP ENDONUCLEASE	coord: 356..455
None	No IPR available	PANTHER	PTHR22748:SF11	DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE CHLOROPLASTIC	coord: 356..455

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002478.1	Spg002478.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
molecular_function	GO:0004518	nuclease activity