Spg002474 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002474
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	trypsin inhibitor 1-like
Location	scaffold6: 4227963 .. 4228208 (+)
RNA-Seq Expression	Spg002474
Synteny	Spg002474

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAGAAAGTTGGGTTGGTGGCGATGGTGGGGATGTTGCTGATGGCGGCTTTGGCTGAGGCTTCCATCGCCGGCGACGGAATTCAGCTAGTGTCCGACGGAGTCAATGATTTTCCAAGAAAAATGATGAATCATGGCAGTCATCATAATAGAATTTGCCCTAGAATCCTTATGGAGTGTTCTTCTGACTCCGATTGCTTGGCTGAGTGCATCTGCCTTGAAAATGGCTTTTGTGGTTGA

mRNA sequence

ATGGAGTGGAAGAAAGTTGGGTTGGTGGCGATGGTGGGGATGTTGCTGATGGCGGCTTTGGCTGAGGCTTCCATCGCCGGCGACGGAATTCAGCTAGTGTCCGACGGAGTCAATGATTTTCCAAGAAAAATGATGAATCATGGCAGTCATCATAATAGAATTTGCCCTAGAATCCTTATGGAGTGTTCTTCTGACTCCGATTGCTTGGCTGAGTGCATCTGCCTTGAAAATGGCTTTTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAGAAAGTTGGGTTGGTGGCGATGGTGGGGATGTTGCTGATGGCGGCTTTGGCTGAGGCTTCCATCGCCGGCGACGGAATTCAGCTAGTGTCCGACGGAGTCAATGATTTTCCAAGAAAAATGATGAATCATGGCAGTCATCATAATAGAATTTGCCCTAGAATCCTTATGGAGTGTTCTTCTGACTCCGATTGCTTGGCTGAGTGCATCTGCCTTGAAAATGGCTTTTGTGGTTGA

Protein sequence

MEWKKVGLVAMVGMLLMAALAEASIAGDGIQLVSDGVNDFPRKMMNHGSHHNRICPRILMECSSDSDCLAECICLENGFCG

Homology

[Close]

BLAST of Spg002474 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 104.0 bits (258), Expect = 6.3e-19
Identity = 55/83 (66.27%), Postives = 61/83 (73.49%), Query Frame = 0


Query: 1   MEWKKVGLVAMVGMLLMAALAEASI--AGDGIQLVSDGVNDFPRKMMNHGSHHNRICPRI 60
           MEWK+  L+AMVG+LLMAA AE++    G+ IQLVSDGVNDFPRKMMN        CPRI
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALG----CPRI 142

Query: 61  LMECSSDSDCLAECICLENGFCG 82
           LMEC  DSDCL  CIC  NGFCG
Sbjct: 143 LMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Spg002474 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 104.0 bits (258), Expect = 6.3e-19
Identity = 55/83 (66.27%), Postives = 61/83 (73.49%), Query Frame = 0


Query: 1   MEWKKVGLVAMVGMLLMAALAEASI--AGDGIQLVSDGVNDFPRKMMNHGSHHNRICPRI 60
           MEWK+  L+AMVG+LLMAA AE++    G+ IQLVSDGVNDFPRKMMN        CPRI
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALG----CPRI 142

Query: 61  LMECSSDSDCLAECICLENGFCG 82
           LMEC  DSDCL  CIC  NGFCG
Sbjct: 143 LMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Spg002474 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 103.6 bits (257), Expect = 8.2e-19
Identity = 55/83 (66.27%), Postives = 61/83 (73.49%), Query Frame = 0


Query: 1  MEWKKVGLVAMVGMLLMAALAEASI--AGDGIQLVSDGVNDFPRKMMNHGSHHNRICPRI 60
          MEWK+  L+AMVG+LLMAA AE++    G+ IQLVSDGVNDFPRKMMN        CPRI
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQG----CPRI 60

Query: 61 LMECSSDSDCLAECICLENGFCG 82
          LMEC  DSDCL  CIC  NGFCG
Sbjct: 61 LMECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Spg002474 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 102.4 bits (254), Expect = 1.8e-18
Identity = 53/83 (63.86%), Postives = 61/83 (73.49%), Query Frame = 0


Query: 1   MEWKKVGLVAMVGMLLMAALAEASI--AGDGIQLVSDGVNDFPRKMMNHGSHHNRICPRI 60
           MEWK+  L+AMVG+LLMAA A+++    G+ IQLVSDGVNDFPRKMMN        CPRI
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALG----CPRI 144

Query: 61  LMECSSDSDCLAECICLENGFCG 82
           LMEC  DSDCL  C+C  NGFCG
Sbjct: 145 LMECEVDSDCLPGCVCRPNGFCG 163

[Close]

BLAST of Spg002474 vs. NCBI nr
Match: XP_031739937.1 (uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 90.5 bits (223), Expect = 7.2e-15
Identity = 45/84 (53.57%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 1  MEWKKVGLVAMVGMLLMAALAE---ASIAGDGIQLVSDGVNDFPRKMMNHGSHHNRICPR 60
          MEWKK+G++A+VGML+MAAL E   A+I  + ++ ++   N+ PRKMMN      R+CPR
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNK--NELPRKMMN---EETRMCPR 60

Query: 61 ILMECSSDSDCLAECICLENGFCG 82
          IL EC++D DC+ +CICL NGFCG
Sbjct: 61 ILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Spg002474 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 8.2e-14
Identity = 35/65 (53.85%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 17 MAALAEASIAGDGIQLVSDGVNDFPRKMMNHGSHHNRICPRILMECSSDSDCLAECICLE 76
          MA++AE+S   + I+L+SDG ND PRK+M+    H  ICPRILM C +D DC+ +C CL 
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMS--GRHGGICPRILMPCKTDDDCMLDCRCLS 60

Query: 77 NGFCG 82
          NG+CG
Sbjct: 61 NGYCG 63

[Close]

BLAST of Spg002474 vs. ExPASy Swiss-Prot
Match: Q9S8I2 (Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.0e-11
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 0


Query: 53 RICPRILMECSSDSDCLAECICLENGFCG 82
          RICPRILMECSSDSDCLAECICLENGFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLENGFCG 29

[Close]

BLAST of Spg002474 vs. ExPASy Swiss-Prot
Match: P25849 (Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-10
Identity = 28/29 (96.55%), Postives = 28/29 (96.55%), Query Frame = 0


Query: 53 RICPRILMECSSDSDCLAECICLENGFCG 82
          RICPRILMECSSDSDCLAECICLE GFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLEQGFCG 29

[Close]

BLAST of Spg002474 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.2e-10
Identity = 36/69 (52.17%), Postives = 42/69 (60.87%), Query Frame = 0


Query: 17 MAALAEASIAGDG----IQLVSDGVNDFPRKMMNHGSHHNRICPRILMECSSDSDCLAEC 76
          MAA  E++ AG G    IQLVSDGVN++  KMM         CPRILM C  + DCL  C
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVV----ACPRILMPCKVNDDCLRGC 60

Query: 77 ICLENGFCG 82
           CL NG+CG
Sbjct: 61 KCLSNGYCG 65

[Close]

BLAST of Spg002474 vs. ExPASy Swiss-Prot
Match: P35628 (Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 9.4e-10
Identity = 27/28 (96.43%), Postives = 27/28 (96.43%), Query Frame = 0


Query: 54 ICPRILMECSSDSDCLAECICLENGFCG 82
          ICPRILM CSSDSDCLAECICLENGFCG
Sbjct: 1  ICPRILMPCSSDSDCLAECICLENGFCG 28

[Close]

BLAST of Spg002474 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.0e-19
Identity = 55/83 (66.27%), Postives = 61/83 (73.49%), Query Frame = 0


Query: 1  MEWKKVGLVAMVGMLLMAALAEASI--AGDGIQLVSDGVNDFPRKMMNHGSHHNRICPRI 60
          MEWK+  L+AMVG+LLMAA AE++    G+ IQLVSDGVNDFPRKMMN        CPRI
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQG----CPRI 60

Query: 61 LMECSSDSDCLAECICLENGFCG 82
          LMEC  DSDCL  CIC  NGFCG
Sbjct: 61 LMECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Spg002474 vs. ExPASy TrEMBL
Match: A0A0A0KT01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.5e-15
Identity = 45/84 (53.57%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 1  MEWKKVGLVAMVGMLLMAALAE---ASIAGDGIQLVSDGVNDFPRKMMNHGSHHNRICPR 60
          MEWKK+G++A+VGML+MAAL E   A+I  + ++ ++   N+ PRKMMN      R+CPR
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNK--NELPRKMMN---EETRMCPR 60

Query: 61 ILMECSSDSDCLAECICLENGFCG 82
          IL EC++D DC+ +CICL NGFCG
Sbjct: 61 ILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Spg002474 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.7e-12
Identity = 45/84 (53.57%), Postives = 55/84 (65.48%), Query Frame = 0


Query: 1  MEWKKVGLVAMVGMLLMAALAEA---SIAGDGIQLVSDGVNDFPRKMMNHGSHHNRICPR 60
          MEWKK+ LVAM+GMLLMA   E+    +  + IQLVSDGVN++        S H   CPR
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNEY--------SGHPG-CPR 60

Query: 61 ILMECSSDSDCLAECICLENGFCG 82
          ILM+C +D DCLA C C  NG+CG
Sbjct: 61 ILMKCKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of Spg002474 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.0e-12
Identity = 43/84 (51.19%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 1  MEWKKVGLVAMVGMLLMAALAEA---SIAGDGIQLVSDGVNDFPRKMMNHGSHHNRICPR 60
          MEWKK+ LVAM+GMLLMA   E+    +  + IQLVSDGVN++ +   N G      CPR
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQ---NIGKDTAPGCPR 60

Query: 61 ILMECSSDSDCLAECICLENGFCG 82
          ILM+C +DSDC   C C   G+CG
Sbjct: 61 ILMKCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Spg002474 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.5e-10
Identity = 42/92 (45.65%), Postives = 57/92 (61.96%), Query Frame = 0


Query: 1  MEWKKVGLVAMVGMLLMAALAEASIA-----GDGIQLVSDG---VNDFPRKMMNHG---S 60
          M+ KK+ +VA+VGM++M A   +S +      D   LVSDG    N  PRK+M  G    
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60

Query: 61 HHNRICPRILMECSSDSDCLAECICLENGFCG 82
             R+CP+ILM+C  DSDCL +C+CL+ GFCG
Sbjct: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

The following BLAST results are available for this feature:

BLAST of Spg002474 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6576915.1	6.3e-19	66.27	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	6.3e-19	66.27	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022984819.1	8.2e-19	66.27	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
XP_023552845.1	1.8e-18	63.86	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]
XP_031739937.1	7.2e-15	53.57	uncharacterized protein LOC105435333 [Cucumis sativus]	[more]

BLAST of Spg002474 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34950	8.2e-14	53.85	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q9S8I2	5.0e-11	100.00	Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P25849	2.5e-10	96.55	Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q43667	3.2e-10	52.17	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
P35628	9.4e-10	96.43	Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of Spg002474 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBL8	4.0e-19	66.27	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A0A0KT01	3.5e-15	53.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
A0A5D3E0W0	2.7e-12	53.57	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]
A0A0A0KY34	8.0e-12	51.19	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A5D3E148	1.5e-10	45.65	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]

BLAST of Spg002474 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PRINTS	PR00293	SQUASHINHBTR	coord: 55..64 score: 73.0 coord: 65..74 score: 80.5
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 53..81 e-value: 3.7E-18 score: 65.2
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 55..74
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 55..81 e-value: 3.55704E-13 score: 55.4615
None	No IPR available	SMART	SM00286	PTI_1	coord: 53..81 e-value: 6.3E-14 score: 62.3
None	No IPR available	GENE3D	4.10.75.20		coord: 26..81 e-value: 1.1E-15 score: 59.1
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 50..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002474.1	Spg002474.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity