Spg002471 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002471
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	scaffold6: 2221575 .. 2222162 (+)
RNA-Seq Expression	Spg002471
Synteny	Spg002471

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAATCCGCCTCCCTCCTCCTTCTCCTCTCCATTTTCACCATCGCCGCCACCACCAGCGCTGCCCGCAAAGGCGGAGCTTCCAATTTCATCAGATCCAGGTGCAGCGCCACAACTTACCCTGACCTCTGCTACCAATCCCTCTCAAATTTCGCCTCAACAATCCAGCAAAACCCTCGCCAATTAGTCCAAACCGCTCTCGTCGTCAGCCTCTCCCGCGCTCAATCCGCCCGATCCTTCGTCTGGAAACTGACCAAGTTCAGCGGATTGAAGCCGAGAGAGTACGCCGCCCTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTGGATCGGCTGAGCAAATCCGTGGAAGAACTGAAGCGCGTCAGCGGATCGAAGCAGAAGGACTTCCTGTGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCCGCCATGACCGACGAGAACACTTGCTCCGATGGATTCGCCGGCACGGCGCTGAATGGGAGAGTCAAGTCCTCGGTTAGGGCTCGCATTGTGGATGTTACTCGCGTGATAAGCAATGCGTTGGCTCTGATTAACGAGTATGCTGAAAATCAAAGCTAG

mRNA sequence

ATGGCGAAATCCGCCTCCCTCCTCCTTCTCCTCTCCATTTTCACCATCGCCGCCACCACCAGCGCTGCCCGCAAAGGCGGAGCTTCCAATTTCATCAGATCCAGGTGCAGCGCCACAACTTACCCTGACCTCTGCTACCAATCCCTCTCAAATTTCGCCTCAACAATCCAGCAAAACCCTCGCCAATTAGTCCAAACCGCTCTCGTCGTCAGCCTCTCCCGCGCTCAATCCGCCCGATCCTTCGTCTGGAAACTGACCAAGTTCAGCGGATTGAAGCCGAGAGAGTACGCCGCCCTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTGGATCGGCTGAGCAAATCCGTGGAAGAACTGAAGCGCGTCAGCGGATCGAAGCAGAAGGACTTCCTGTGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCCGCCATGACCGACGAGAACACTTGCTCCGATGGATTCGCCGGCACGGCGCTGAATGGGAGAGTCAAGTCCTCGGTTAGGGCTCGCATTGTGGATGTTACTCGCGTGATAAGCAATGCGTTGGCTCTGATTAACGAGTATGCTGAAAATCAAAGCTAG

Coding sequence (CDS)

ATGGCGAAATCCGCCTCCCTCCTCCTTCTCCTCTCCATTTTCACCATCGCCGCCACCACCAGCGCTGCCCGCAAAGGCGGAGCTTCCAATTTCATCAGATCCAGGTGCAGCGCCACAACTTACCCTGACCTCTGCTACCAATCCCTCTCAAATTTCGCCTCAACAATCCAGCAAAACCCTCGCCAATTAGTCCAAACCGCTCTCGTCGTCAGCCTCTCCCGCGCTCAATCCGCCCGATCCTTCGTCTGGAAACTGACCAAGTTCAGCGGATTGAAGCCGAGAGAGTACGCCGCCCTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTGGATCGGCTGAGCAAATCCGTGGAAGAACTGAAGCGCGTCAGCGGATCGAAGCAGAAGGACTTCCTGTGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCCGCCATGACCGACGAGAACACTTGCTCCGATGGATTCGCCGGCACGGCGCTGAATGGGAGAGTCAAGTCCTCGGTTAGGGCTCGCATTGTGGATGTTACTCGCGTGATAAGCAATGCGTTGGCTCTGATTAACGAGTATGCTGAAAATCAAAGCTAG

Protein sequence

MAKSASLLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEELKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISNALALINEYAENQS

Homology

[Close]

BLAST of Spg002471 vs. NCBI nr
Match: XP_022922994.1 (21 kDa protein-like [Cucurbita moschata] >XP_022985513.1 21 kDa protein-like [Cucurbita maxima] >KAG7014820.1 hypothetical protein SDJN02_22449, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.0 bits (863), Expect = 1.1e-88
Identity = 175/195 (89.74%), Postives = 187/195 (95.90%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNP 60
           MAKSASLLLLLSI TIAATTSAAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQNP
Sbjct: 1   MAKSASLLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEEL 120
           RQLVQTALVVSLSRAQS RSFVWK+TKFSGLK REYAAL+DCMEEVGDTVDRLSKSVEEL
Sbjct: 61  RQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEEL 120

Query: 121 KRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVI 180
           KRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RVI
Sbjct: 121 KRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVI 180

Query: 181 SNALALINEYAENQS 196
           SNAL+LIN+YAEN+S
Sbjct: 181 SNALSLINKYAENES 195

[Close]

BLAST of Spg002471 vs. NCBI nr
Match: XP_038882396.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 333.6 bits (854), Expect = 1.2e-87
Identity = 176/196 (89.80%), Postives = 185/196 (94.39%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTIAAT-TSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAK ASLLLLLSI TIAAT T+ AR GGASNFI+S CS  TYPDLC QSLSNFASTIQQN
Sbjct: 1   MAKPASLLLLLSILTIAATATATARNGGASNFIKSECSVATYPDLCVQSLSNFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL+VSLSRAQS RSFVWKLTKFSGLKPRE+AALKDCMEEV DTVDRL+KSVEE
Sbjct: 61  PRQLVQTALIVSLSRAQSTRSFVWKLTKFSGLKPREHAALKDCMEEVSDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGR+KSSVRARIVDVTRV
Sbjct: 121 LKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRIKSSVRARIVDVTRV 180

Query: 181 ISNALALINEYAENQS 196
           ISNAL+LIN+YAENQS
Sbjct: 181 ISNALSLINKYAENQS 196

[Close]

BLAST of Spg002471 vs. NCBI nr
Match: XP_023551839.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 332.4 bits (851), Expect = 2.6e-87
Identity = 175/196 (89.29%), Postives = 187/196 (95.41%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTI-AATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKSASLLLLLSI TI AATTSAAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQN
Sbjct: 1   MAKSASLLLLLSILTIAAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKFSGLK REYAAL+DCMEEVGDTVDRLSKSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALALINEYAENQS 196
           ISNAL+LIN+YAEN+S
Sbjct: 181 ISNALSLINKYAENES 196

[Close]

BLAST of Spg002471 vs. NCBI nr
Match: XP_008439100.1 (PREDICTED: 21 kDa protein-like [Cucumis melo] >KAA0067679.1 21 kDa protein-like [Cucumis melo var. makuwa] >TYK23683.1 21 kDa protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 313.9 bits (803), Expect = 9.6e-82
Identity = 169/196 (86.22%), Postives = 181/196 (92.35%), Query Frame = 0


Query: 1   MAKSAS-LLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKS S LLLLLSI TI+A   +A  GGASNFI+S+CSA TYPDLC QSLS+FASTIQ+N
Sbjct: 1   MAKSLSLLLLLLSILTISA---SAFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLSRAQS RSFVWKLTKFSGLKPRE AALKDCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAG+ALNGR+KSSVR RIVDVTRV
Sbjct: 121 LKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALALINEYAENQS 196
           ISNAL+LIN+YAE QS
Sbjct: 181 ISNALSLINKYAETQS 193

[Close]

BLAST of Spg002471 vs. NCBI nr
Match: XP_022140842.1 (21 kDa protein-like [Momordica charantia])

HSP 1 Score: 312.0 bits (798), Expect = 3.6e-81
Identity = 161/194 (82.99%), Postives = 179/194 (92.27%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNP 60
           MA+SA LL+LLSI ++AAT+ AA   GASNFI+S+CSA TYPDLC QSLSNFASTIQQNP
Sbjct: 1   MARSAFLLVLLSILSVAATSGAAGNAGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEEL 120
           RQLVQTAL VSLSRAQSARS VWKLTKF GL+PRE+AALKDC+EEV DTVDRLSKSVEEL
Sbjct: 61  RQLVQTALFVSLSRAQSARSHVWKLTKFEGLRPREHAALKDCLEEVSDTVDRLSKSVEEL 120

Query: 121 KRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVI 180
           KRV G+ +KDFLWHISNVETWVSAAMTDENTCSDGF+G A+NGR+KSSVRARIVDV+RVI
Sbjct: 121 KRVHGAHEKDFLWHISNVETWVSAAMTDENTCSDGFSGKAMNGRIKSSVRARIVDVSRVI 180

Query: 181 SNALALINEYAENQ 195
           SNALALI++YAENQ
Sbjct: 181 SNALALISKYAENQ 194

[Close]

BLAST of Spg002471 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-49
Identity = 103/191 (53.93%), Postives = 141/191 (73.82%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNP 60
           M  S S L+  ++  I A  SAA    A+ FI++ C+ TTYP +C QSLS +A TIQ NP
Sbjct: 1   MKLSKSTLVFSALLVILAAASAA---PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEEL 120
           ++L  TAL VSL+R Q A++F+ +L KF GLK R+YAA+ DC+EEV D++DR+S+S +E+
Sbjct: 61  QELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEM 120

Query: 121 KRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVI 180
           K +S +K  DF + +SNVETWVSAA+TDE TC DGFAG  ++G++K SVRA++V V RV 
Sbjct: 121 KNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVT 180

Query: 181 SNALALINEYA 192
           SNALAL+N +A
Sbjct: 181 SNALALVNNFA 188

[Close]

BLAST of Spg002471 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 2.3e-46
Identity = 98/190 (51.58%), Postives = 139/190 (73.16%), Query Frame = 0


Query: 8   LLLLSIFTIAATTSAARKG----GASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQL 67
           L LLS   + A++SA R       A NFI++ C ATTYP +C  SL+ +A++IQ +PR+L
Sbjct: 11  LFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRL 70

Query: 68  VQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEELKRV 127
            +TAL V++++AQS + FVW+L +F+ LK RE  A+KDC+EE+ D VDRL+ S+ E+K  
Sbjct: 71  AETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMC 130

Query: 128 SGSKQKDFLW-HISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISN 187
             +K +D  W H+SN +TW SAA+T+ NTCSDGFAG  ++GRVK+SVRARI+++ R  SN
Sbjct: 131 GSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSN 190

Query: 188 ALALINEYAE 193
           ALALIN +A+
Sbjct: 191 ALALINAFAK 200

[Close]

BLAST of Spg002471 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.6e-42
Identity = 91/198 (45.96%), Postives = 134/198 (67.68%), Query Frame = 0


Query: 1   MAKSASLLLLLSIF------TIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFAS 60
           MA++  L L+L +        + A        G + FI++ C  T+YPD C+QSLS++AS
Sbjct: 1   MARNFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYAS 60

Query: 61  TIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLS 120
            I++ PR+L +TAL VS++RA+SA+++V ++T + G+  R++ A+ DC+EE+GDTVDRLS
Sbjct: 61  EIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLS 120

Query: 121 KSVEELKRV-SGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARI 180
            S++ELK +  G   +DF + +SNV TW SAA+TDE  C DGF G A+ G +KS +R  I
Sbjct: 121 NSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHI 180

Query: 181 VDVTRVISNALALINEYA 192
           V V    SNALALIN++A
Sbjct: 181 VSVAEETSNALALINDFA 198

[Close]

BLAST of Spg002471 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.9e-41
Identity = 93/192 (48.44%), Postives = 136/192 (70.83%), Query Frame = 0


Query: 7   LLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQ-LVQ 66
           L++LLSI  + ++++   +  +  FI S C  T YP LC  +LS +A+ I+ N  Q L Q
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 67  TALVVSLSRAQSARSFVWKLTKFS-GLKPREYAALKDCMEEVGDTVDRLSKSVEELKRVS 126
           TAL++SL+RA+S   FV KLTK +   K REY A+KDC+E +G++VDRL++SV+EL R  
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 127 GS-KQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISNA 186
            +   +DF+W +SNV+TWVSAA+TDE TC DGF+  A+ G+VK  +R ++V V +V SNA
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

Query: 187 LALINEYAENQS 196
           LAL+N++AE +S
Sbjct: 189 LALVNQFAEKRS 200

[Close]

BLAST of Spg002471 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 7.2e-32
Identity = 81/198 (40.91%), Postives = 120/198 (60.61%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTI--AATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQ 60
           MA + +L L+   F +   A+T   R  GA + + S C   +YP LC ++LS+++     
Sbjct: 1   MAPTQNLFLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTIT 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVE 120
           N R L Q A+ +SLS AQSA   +  +    G K +E AAL DC+E +GD+VD LS+++ 
Sbjct: 61  NRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLG 120

Query: 121 ELK--RVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDV 180
            LK  RVSG   K+F W +SN +TW SAA+TD++TC DGF G   +G +K+ V+  +  V
Sbjct: 121 VLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMD-DGEIKTEVKQWMTKV 180

Query: 181 TRVISNALALINEYAENQ 195
            RV SNAL ++N+  E +
Sbjct: 181 ARVTSNALYMVNQLDETR 197

[Close]

BLAST of Spg002471 vs. ExPASy TrEMBL
Match: A0A6J1JDT9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483500 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 5.1e-89
Identity = 175/195 (89.74%), Postives = 187/195 (95.90%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNP 60
           MAKSASLLLLLSI TIAATTSAAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQNP
Sbjct: 1   MAKSASLLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEEL 120
           RQLVQTALVVSLSRAQS RSFVWK+TKFSGLK REYAAL+DCMEEVGDTVDRLSKSVEEL
Sbjct: 61  RQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEEL 120

Query: 121 KRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVI 180
           KRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RVI
Sbjct: 121 KRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVI 180

Query: 181 SNALALINEYAENQS 196
           SNAL+LIN+YAEN+S
Sbjct: 181 SNALSLINKYAENES 195

[Close]

BLAST of Spg002471 vs. ExPASy TrEMBL
Match: A0A6J1E526 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430812 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 5.1e-89
Identity = 175/195 (89.74%), Postives = 187/195 (95.90%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNP 60
           MAKSASLLLLLSI TIAATTSAAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQNP
Sbjct: 1   MAKSASLLLLLSILTIAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEEL 120
           RQLVQTALVVSLSRAQS RSFVWK+TKFSGLK REYAAL+DCMEEVGDTVDRLSKSVEEL
Sbjct: 61  RQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEEL 120

Query: 121 KRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVI 180
           KRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RVI
Sbjct: 121 KRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRVI 180

Query: 181 SNALALINEYAENQS 196
           SNAL+LIN+YAEN+S
Sbjct: 181 SNALSLINKYAENES 195

[Close]

BLAST of Spg002471 vs. ExPASy TrEMBL
Match: A0A5A7VRA6 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00490 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.6e-82
Identity = 169/196 (86.22%), Postives = 181/196 (92.35%), Query Frame = 0


Query: 1   MAKSAS-LLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKS S LLLLLSI TI+A   +A  GGASNFI+S+CSA TYPDLC QSLS+FASTIQ+N
Sbjct: 1   MAKSLSLLLLLLSILTISA---SAFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLSRAQS RSFVWKLTKFSGLKPRE AALKDCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAG+ALNGR+KSSVR RIVDVTRV
Sbjct: 121 LKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALALINEYAENQS 196
           ISNAL+LIN+YAE QS
Sbjct: 181 ISNALSLINKYAETQS 193

[Close]

BLAST of Spg002471 vs. ExPASy TrEMBL
Match: A0A1S3AXX1 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483991 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.6e-82
Identity = 169/196 (86.22%), Postives = 181/196 (92.35%), Query Frame = 0


Query: 1   MAKSAS-LLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKS S LLLLLSI TI+A   +A  GGASNFI+S+CSA TYPDLC QSLS+FASTIQ+N
Sbjct: 1   MAKSLSLLLLLLSILTISA---SAFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQRN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLSRAQS RSFVWKLTKFSGLKPRE AALKDCMEEVGDTVDRL+KSVEE
Sbjct: 61  PRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAG+ALNGR+KSSVR RIVDVTRV
Sbjct: 121 LKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTRV 180

Query: 181 ISNALALINEYAENQS 196
           ISNAL+LIN+YAE QS
Sbjct: 181 ISNALSLINKYAETQS 193

[Close]

BLAST of Spg002471 vs. ExPASy TrEMBL
Match: A0A6J1CI81 (21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011412 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.8e-81
Identity = 161/194 (82.99%), Postives = 179/194 (92.27%), Query Frame = 0


Query: 1   MAKSASLLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNP 60
           MA+SA LL+LLSI ++AAT+ AA   GASNFI+S+CSA TYPDLC QSLSNFASTIQQNP
Sbjct: 1   MARSAFLLVLLSILSVAATSGAAGNAGASNFIKSKCSAATYPDLCVQSLSNFASTIQQNP 60

Query: 61  RQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEEL 120
           RQLVQTAL VSLSRAQSARS VWKLTKF GL+PRE+AALKDC+EEV DTVDRLSKSVEEL
Sbjct: 61  RQLVQTALFVSLSRAQSARSHVWKLTKFEGLRPREHAALKDCLEEVSDTVDRLSKSVEEL 120

Query: 121 KRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVI 180
           KRV G+ +KDFLWHISNVETWVSAAMTDENTCSDGF+G A+NGR+KSSVRARIVDV+RVI
Sbjct: 121 KRVHGAHEKDFLWHISNVETWVSAAMTDENTCSDGFSGKAMNGRIKSSVRARIVDVSRVI 180

Query: 181 SNALALINEYAENQ 195
           SNALALI++YAENQ
Sbjct: 181 SNALALISKYAENQ 194

[Close]

BLAST of Spg002471 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 194.9 bits (494), Expect = 6.0e-50
Identity = 104/187 (55.61%), Postives = 141/187 (75.40%), Query Frame = 0


Query: 7   LLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQLVQT 66
           L L L+  T A+ T A++K  A NFI+S C  TTYP LC  SLS +A+ IQ +P++L +T
Sbjct: 16  LSLELTAATAASQTGASKK--AINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAET 75

Query: 67  ALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEELKRVSGS 126
           A+ V+LSRAQS + FV +LT+  GLK RE  A+KDC+EE+ DTVDRL+KSV+ELK    +
Sbjct: 76  AIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSA 135

Query: 127 KQKD-FLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISNALA 186
           K +D F +H+SN +TW SAA+TDENTCSDGF+G  ++GR+K+SVRARI++V    SNAL+
Sbjct: 136 KDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALS 195

Query: 187 LINEYAE 193
           LIN +A+
Sbjct: 196 LINAFAK 200

[Close]

BLAST of Spg002471 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 1.6e-47
Identity = 98/190 (51.58%), Postives = 139/190 (73.16%), Query Frame = 0


Query: 8   LLLLSIFTIAATTSAARKG----GASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQL 67
           L LLS   + A++SA R       A NFI++ C ATTYP +C  SL+ +A++IQ +PR+L
Sbjct: 11  LFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRL 70

Query: 68  VQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEELKRV 127
            +TAL V++++AQS + FVW+L +F+ LK RE  A+KDC+EE+ D VDRL+ S+ E+K  
Sbjct: 71  AETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMC 130

Query: 128 SGSKQKDFLW-HISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISN 187
             +K +D  W H+SN +TW SAA+T+ NTCSDGFAG  ++GRVK+SVRARI+++ R  SN
Sbjct: 131 GSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSN 190

Query: 188 ALALINEYAE 193
           ALALIN +A+
Sbjct: 191 ALALINAFAK 200

[Close]

BLAST of Spg002471 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 173.3 bits (438), Expect = 1.9e-43
Identity = 96/186 (51.61%), Postives = 126/186 (67.74%), Query Frame = 0


Query: 8   LLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQLVQTA 67
           L LL   TIA  +S+     + NFI S C  T Y  LC + L+ FA  I++N  QL QTA
Sbjct: 16  LPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTA 75

Query: 68  LVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLSKSVEELKRV--SG 127
           L V+L R QS   +V KLTK   +K REY A+KDC+E +GD ++ L++S+ ELK+V  SG
Sbjct: 76  LAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSG 135

Query: 128 SKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISNALA 187
             + +FLW +SNVETWVSAA+TDE TC DGF G  ++G VKS++R R+V V RV SNALA
Sbjct: 136 RDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALA 195

Query: 188 LINEYA 192
           L+N +A
Sbjct: 196 LVNRFA 201

[Close]

BLAST of Spg002471 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.9e-43
Identity = 91/198 (45.96%), Postives = 134/198 (67.68%), Query Frame = 0


Query: 1   MAKSASLLLLLSIF------TIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFAS 60
           MA++  L L+L +        + A        G + FI++ C  T+YPD C+QSLS++AS
Sbjct: 1   MARNFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYAS 60

Query: 61  TIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFSGLKPREYAALKDCMEEVGDTVDRLS 120
            I++ PR+L +TAL VS++RA+SA+++V ++T + G+  R++ A+ DC+EE+GDTVDRLS
Sbjct: 61  EIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLS 120

Query: 121 KSVEELKRV-SGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARI 180
            S++ELK +  G   +DF + +SNV TW SAA+TDE  C DGF G A+ G +KS +R  I
Sbjct: 121 NSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHI 180

Query: 181 VDVTRVISNALALINEYA 192
           V V    SNALALIN++A
Sbjct: 181 VSVAEETSNALALINDFA 198

[Close]

BLAST of Spg002471 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 2.1e-42
Identity = 93/192 (48.44%), Postives = 136/192 (70.83%), Query Frame = 0


Query: 7   LLLLLSIFTIAATTSAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQ-LVQ 66
           L++LLSI  + ++++   +  +  FI S C  T YP LC  +LS +A+ I+ N  Q L Q
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 67  TALVVSLSRAQSARSFVWKLTKFS-GLKPREYAALKDCMEEVGDTVDRLSKSVEELKRVS 126
           TAL++SL+RA+S   FV KLTK +   K REY A+KDC+E +G++VDRL++SV+EL R  
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 127 GS-KQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISNA 186
            +   +DF+W +SNV+TWVSAA+TDE TC DGF+  A+ G+VK  +R ++V V +V SNA
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

Query: 187 LALINEYAENQS 196
           LAL+N++AE +S
Sbjct: 189 LALVNQFAEKRS 200

The following BLAST results are available for this feature:

BLAST of Spg002471 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922994.1	1.1e-88	89.74	21 kDa protein-like [Cucurbita moschata] >XP_022985513.1 21 kDa protein-like [Cu...	[more]
XP_038882396.1	1.2e-87	89.80	21 kDa protein-like [Benincasa hispida]	[more]
XP_023551839.1	2.6e-87	89.29	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_008439100.1	9.6e-82	86.22	PREDICTED: 21 kDa protein-like [Cucumis melo] >KAA0067679.1 21 kDa protein-like ...	[more]
XP_022140842.1	3.6e-81	82.99	21 kDa protein-like [Momordica charantia]	[more]

BLAST of Spg002471 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	1.7e-49	53.93	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9STY5	2.3e-46	51.58	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SB37	2.6e-42	45.96	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI72	2.9e-41	48.44	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q84WE4	7.2e-32	40.91	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]

BLAST of Spg002471 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDT9	5.1e-89	89.74	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483500 PE=4 SV=1	[more]
A0A6J1E526	5.1e-89	89.74	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430812 PE=4 SV=1	[more]
A0A5A7VRA6	4.6e-82	86.22	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G...	[more]
A0A1S3AXX1	4.6e-82	86.22	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483991 PE=4 SV=1	[more]
A0A6J1CI81	1.8e-81	82.99	21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011412 PE=4 SV=1	[more]

BLAST of Spg002471 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62350.1	6.0e-50	55.61	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	1.6e-47	51.58	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	1.9e-43	51.61	pectin methylesterase inhibitor 1	[more]
AT4G25260.1	1.9e-43	45.96	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	2.1e-42	48.44	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 103..123
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 8..193
None	No IPR available	PANTHER	PTHR31080:SF87	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 8..193
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 36..191 e-value: 6.34488E-50 score: 157.215
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..186 e-value: 1.5E-55 score: 200.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..186 e-value: 1.4E-38 score: 132.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..190 e-value: 3.4E-43 score: 145.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..190 e-value: 2.0E-43 score: 150.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 29..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002471.1	Spg002471.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0009641	shade avoidance
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity