Spg002423 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg002423 vs. NCBI nr
Match: XP_022945852.1 (cytochrome P450 714C2-like [Cucurbita moschata])

HSP 1 Score: 886.7 bits (2290), Expect = 1.0e-253
Identity = 433/512 (84.57%), Postives = 468/512 (91.41%), Query Frame = 0

Query: 34  VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
           V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMK+  KA+AAING + 
Sbjct: 14  VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73

Query: 94  ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
           +  S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74  SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133

Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
           GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193

Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
           SSLVNSA  EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253

Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
           PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSDLLSRLIE +++   GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313

Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
           KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373

Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
            MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433

Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
           WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493

Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

BLAST of Spg002423 vs. NCBI nr
Match: KAG6574181.1 (Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 886.3 bits (2289), Expect = 1.3e-253
Identity = 433/512 (84.57%), Postives = 468/512 (91.41%), Query Frame = 0

Query: 34  VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
           V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMK+  KA+AAING + 
Sbjct: 14  VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73

Query: 94  ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
           +  S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74  SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133

Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
           GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193

Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
           SSLVNSA  EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAKASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253

Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
           PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSDLLSRLIE +++   GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313

Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
           KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373

Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
            MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433

Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
           WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493

Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

BLAST of Spg002423 vs. NCBI nr
Match: XP_022968014.1 (cytochrome P450 714C2-like [Cucurbita maxima])

HSP 1 Score: 881.3 bits (2276), Expect = 4.2e-252
Identity = 434/512 (84.77%), Postives = 467/512 (91.21%), Query Frame = 0

Query: 34  VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
           V IMVLFVKVV+SWW+LP+LAHQKLKRNGF GP P+FPLGNIAEMK+  KA+AAING S 
Sbjct: 14  VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPTFPLGNIAEMKRIRKASAAINGDSH 73

Query: 94  ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
           +  S+ VSHDIHS VFPHFAQWQASYGKKFVYWLGTEPFLYIADP F+K+IS AVM KGW
Sbjct: 74  SFPSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVMAKGW 133

Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
           GKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMTKFMVESTT+MLN+W
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNEW 193

Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
           SSLVNSA  EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNSTYVGV 253

Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
           PFS LF CP RTLAAKRLGA IDRLFLS++SERK S+  +SSSDLLSRLIE Q++    G
Sbjct: 254 PFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSDLLSRLIEGQKSTGDVG 313

Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
           KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373

Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
            MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMHRDPDL 433

Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
           WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLILSRFS 493

Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

BLAST of Spg002423 vs. NCBI nr
Match: XP_023542056.1 (cytochrome P450 714C2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 878.6 bits (2269), Expect = 2.7e-251
Identity = 431/512 (84.18%), Postives = 465/512 (90.82%), Query Frame = 0

Query: 34  VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
           V +MVLF+KVV+SWW+LP+LA QKLKRNGF GP PSFPLGNIAEMK+  KA+AAING   
Sbjct: 14  VLVMVLFLKVVVSWWVLPVLALQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDPH 73

Query: 94  ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
           +  S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74  SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133

Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
           GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193

Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
           SSLVNSA  EIDVER ITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVERGITATAGDIIAKASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253

Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
           PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSDLLSRLIE +++   GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313

Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
           KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373

Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
            MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433

Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
           WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493

Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

BLAST of Spg002423 vs. NCBI nr
Match: XP_022141037.1 (cytokinin hydroxylase [Momordica charantia])

HSP 1 Score: 875.9 bits (2262), Expect = 1.8e-250
Identity = 435/523 (83.17%), Postives = 475/523 (90.82%), Query Frame = 0

Query: 33  RVSIMVLFVKVVISWWILPILAHQKLKRNGFE-GPSPSFPLGNIAEMKKTMK------AA 92
           RVS++VL V+VV SWWILP+LAH+KLKRNGF  GP P FPLGNI E+K TMK      AA
Sbjct: 13  RVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 72

Query: 93  AAINGGSFA-SSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI 152
           AAINGG+ A SSS+N+SHDIHS VFP+FAQWQ SYGKKFVYWLGTEPFLYIA+PQFVK +
Sbjct: 73  AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 132

Query: 153 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVE 212
           SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMT+ MV 
Sbjct: 133 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVG 192

Query: 213 STTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTL 272
           STT+ML++W+  ++S  PE+DVEREITATAGDIIA+ASFGI +  GRDAFN LR LQFTL
Sbjct: 193 STTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTL 252

Query: 273 FQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIE 332
           F+NS+YVGVPFS +FL P RTL AKRLG+EIDRLFLS+ISERKQS + +S+SDLLSRLIE
Sbjct: 253 FRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD-ASASDLLSRLIE 312

Query: 333 SQRNGS--GGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEI 392
           SQRN S    GK  LTTRELVDECKTFFFGGHETTALAISWT+LLLATRPEWQ ILREEI
Sbjct: 313 SQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEI 372

Query: 393 KEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWI 452
           KEVIGD+EIDFAMLS L+KMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD TIPKGTNIWI
Sbjct: 373 KEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWI 432

Query: 453 DVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYK 512
           D+V+MHHDP+LWG+DVNEFKPERFEKDSIHGGC HKMGYLPFGFGGRMCIGRNLSSMEYK
Sbjct: 433 DIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYK 492

Query: 513 IVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           IVLTLI+SRFSLS+SPSYSHSPAILLSLRPAHGLPLI+KPL P
Sbjct: 493 IVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534

BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 4.7e-89
Identity = 185/518 (35.71%), Postives = 283/518 (54.63%), Query Frame = 0

Query: 34  VSIMVLFVKV----VISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAIN 93
           V +M L ++V    +  +++ P    + ++R G  GP P    GNI ++ K +  +A   
Sbjct: 12  VIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSA--- 71

Query: 94  GGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI---SE 153
               ++  +++ H+I   + PH+  W   YGK+F+ W GTEP L + + + +K++     
Sbjct: 72  ----SNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHN 131

Query: 154 AVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVEST 213
            V GK W    + +   +   G GL+M  G+ W   RH+  PAF    LK   K MVE T
Sbjct: 132 PVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 191

Query: 214 TQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQ 273
             M  +   L      E+++  E+     DII++  FG S   G++ F+ L  LQ    Q
Sbjct: 192 KMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 251

Query: 274 NSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQ 333
            + ++  P S  FL        K L  E++RL + +I  RK S E   SS     L+   
Sbjct: 252 ATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLL 311

Query: 334 RNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVI 393
            N     K  L  + ++DECKTFFF GHETT+L ++WTL+LLA  P WQ+ +R+E+++V 
Sbjct: 312 LNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVC 371

Query: 394 GDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 453
           G   +     LS L  +  V NE LRLYP A  + R A  DIK+GD+ IPKG +IWI V+
Sbjct: 372 GQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVL 431

Query: 454 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 513
           ++HH   LWGED NEF PERF   S      H   ++PF  G R CIG+  + ME KI+L
Sbjct: 432 AIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFAMMEAKIIL 491

Query: 514 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
            +++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 492 AMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 8.1e-89
Identity = 182/511 (35.62%), Postives = 277/511 (54.21%), Query Frame = 0

Query: 37  MVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASS 96
           ++LF  V I  W+ P    QKL+  G  GP PSF  GNI EM++  + A + +    A S
Sbjct: 20  ILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQE-AGS 79

Query: 97  SNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKP 156
           ++  S +  + +FP+F  W   YG  ++Y  G+   L + DP  VK+++        GKP
Sbjct: 80  TDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANC-KSLDLGKP 139

Query: 157 AVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSL 216
              + +R  + G G++ + GD W+  R ++ P      +K M   M+E+   MLN W + 
Sbjct: 140 CYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNE 199

Query: 217 V--NSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVP 276
           V       EI V+  +   + D+I++A FG S   G++ F  +R LQ  + + S  +GVP
Sbjct: 200 VEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGVP 259

Query: 277 FSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGK 336
            S  +L          L + I  L L++  + +     S + DLL  +I+    GS  G 
Sbjct: 260 GS-RYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVNKDLLHSIIQ----GSKDGP 319

Query: 337 MRLTTRE--LVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEID 396
               T E  +VD CK  +F GHETT+   +W L+LLA+  EWQ+  R E  ++   + +D
Sbjct: 320 FASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLD 379

Query: 397 FAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPT 456
           F +L  L+K+  V  E LRLYP A  + R+A  D+K+G + IPKGTNIWI +   H DP+
Sbjct: 380 FDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPS 439

Query: 457 LWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRF 516
           +WG   ++F P+RF  + I G C     Y+PFG G R C G+NL+ +E K+VL+L+LS+F
Sbjct: 440 VWGPSADKFDPDRF-ANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKF 499

Query: 517 SLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
              LSP+Y H PA  L++ P  G+PLI + L
Sbjct: 500 EFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.1e-88
Identity = 184/527 (34.91%), Postives = 284/527 (53.89%), Query Frame = 0

Query: 35  SIMVLFVKVVI--------SWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAA 94
           S++V+FV  ++         +W+ P    + +++ G  GP P    GNI E+       +
Sbjct: 8   SLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI-------S 67

Query: 95  AINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI-- 154
           A+   S +   +++ HDI   + PH+  W   YGK+F+ W GT+P L + + + +K++  
Sbjct: 68  AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 127

Query: 155 -SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMV 214
               V G+ W +    +N      G GL+M  G DW   RH+  PAF    LK   + MV
Sbjct: 128 KHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMV 187

Query: 215 ESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFT 274
           E T++++ +    V     E+++  E+     DII++  FG S   G++ FN L  LQ  
Sbjct: 188 ECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRR 247

Query: 275 LFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS----DLL 334
             Q + ++  P S  FL        K L  E++RL + +I  R+   E   SS    DLL
Sbjct: 248 CAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 307

Query: 335 SRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILR 394
             L+                + ++DECKTFFF GHETTAL ++WT +LLA  P WQ  +R
Sbjct: 308 GLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVR 367

Query: 395 EEIKEVIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGT 454
           EE++EV G   +     LS L  +  V NE LRLYP A  + R A  D+K+GD+TIPKG 
Sbjct: 368 EEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGL 427

Query: 455 NIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMCIGRNL 514
           +IWI V+++HH   LWG+D N+F PERF      GG     G  ++PF  G R CIG+  
Sbjct: 428 SIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCIGQQF 487

Query: 515 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
           + ME KI+L  ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 488 ALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 3.9e-83
Identity = 176/504 (34.92%), Postives = 269/504 (53.37%), Query Frame = 0

Query: 48  WILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSS--NNVSHDIH 107
           W++P     K +     GP PSFP GN+A+M++   AA A    +  S S    + HD  
Sbjct: 32  WLVPQKVLAKFEDQKIGGPRPSFPYGNLADMREAAAAAKAARASARRSGSGGGGIVHDYR 91

Query: 108 SIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAVFRNDREP 167
             V P++ +W+  YG  F Y +G   FL+++ P  V+ I+  V     GK +  +   EP
Sbjct: 92  PAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYLKATHEP 151

Query: 168 MFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLV--NSAQPE 227
           +FG G++ + G+ W+  R I+ P F    +K M   MV+S   +L  W   V  N    +
Sbjct: 152 LFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDRNGGITD 211

Query: 228 IDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPFSGLFLCPA 287
           I ++ +I A + D+I++  FG S   G++ F  +R LQ  +  +   V    +GL   P+
Sbjct: 212 IKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAV--SKPNVLAEMTGLRFFPS 271

Query: 288 -RTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGKMRLTTREL 347
            R   A  L  ++ +L L ++      +E+    +LLS ++ S      G  +      +
Sbjct: 272 MRNKQAWELHKQVRKLILEIV------KESGEDRNLLSAILHSASTSRVG--IAEAENFI 331

Query: 348 VDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDFAMLSGLRKM 407
           VD CK+ +F GHE+TA+  +W L+LL   PEWQN +REE+ EV   + +D   L  ++ +
Sbjct: 332 VDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRSLQKMKNL 391

Query: 408 GWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFK 467
             V  E LRLYP+   + RQA  ++K+G V IPKG NI+I V +MH DP LWG DV EF 
Sbjct: 392 TMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWGPDVKEFN 451

Query: 468 PERFE--KDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFSLSLSPSY 527
           PERF   +  +H        YLPFG G R C+G+  +  E KI+++LI+S+F L LSP Y
Sbjct: 452 PERFSDVRPQLH-------SYLPFGAGARTCLGQGFAMAELKILISLIVSKFVLKLSPHY 511

Query: 528 SHSPAILLSLRPAHGLPLIVKPLQ 545
            HSP + L + P  G+ L +  +Q
Sbjct: 512 QHSPTLKLIVEPELGVDLTLTKVQ 517

BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match: Q7XHW5 (Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714B1 PE=1 SV=2)

HSP 1 Score: 307.0 bits (785), Expect = 4.3e-82
Identity = 170/511 (33.27%), Postives = 273/511 (53.42%), Query Frame = 0

Query: 48  WILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSSNNVSHDIHSI 107
           W+ P      L+R G  GP+PSFP GN+A+M+    AAA            ++ HD    
Sbjct: 27  WLAPERLRAHLRRQGIGGPTPSFPYGNLADMRSHAAAAAGGKATGEGRQEGDIVHDYRQA 86

Query: 108 VFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAVFRNDREPMF 167
           VFP +  W+  YG  F Y +G   FL+++ P  V+++S  V     GK +  +   +P+F
Sbjct: 87  VFPFYENWRKQYGPVFTYSVGNMVFLHVSRPDIVRELSLCV-SLDLGKSSYMKATHQPLF 146

Query: 168 GDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVNSA---QPEI 227
           G+G++ + G+ W   R ++ P F P  +K M   MV+S   +++ W   ++ +     ++
Sbjct: 147 GEGILKSNGNAWAHQRKLIAPEFFPDKVKGMVDLMVDSAQVLVSSWEDRIDRSGGNALDL 206

Query: 228 DVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPFSGL-FLCP- 287
            ++ +I A + D+I++  FG S   G+  F+ +R LQ T+      +    +GL FL P 
Sbjct: 207 MIDDDIRAYSADVISRTCFGSSYVKGKQIFDMIRELQKTVSTKKQNLLAEMTGLSFLFPK 266

Query: 288 ARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGKMRLTTRE- 347
           A   AA RL   +  L L ++ E  + +      +LLS ++ S R G GGG       E 
Sbjct: 267 ASGRAAWRLNGRVRALILDLVGENGEED----GGNLLSAMLRSARGGGGGGGEVAAAAED 326

Query: 348 -LVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVI---GDKEIDFAMLS 407
            +VD CK  +F G+E+TA+  +W L+LLA  PEWQ+ +R+E++      G +  DF  L 
Sbjct: 327 FVVDNCKNIYFAGYESTAVTAAWCLMLLALHPEWQDRVRDEVQAACCGGGGRSPDFPALQ 386

Query: 408 GLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGED 467
            ++ +  V  E LRLYP+   + RQA  ++ +G V +P+G NI++ V ++H D  LWG  
Sbjct: 387 KMKNLTMVIQETLRLYPAGAVVSRQALRELSLGGVRVPRGVNIYVPVSTLHLDAELWGGG 446

Query: 468 VN--EFKPERF--EKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 527
               EF P RF   +  +H        YLPFG G R C+G+  +  E K++L+L+L RF 
Sbjct: 447 AGAAEFDPARFADARPPLH-------AYLPFGAGARTCLGQTFAMAELKVLLSLVLCRFE 506

Query: 528 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQ 545
           ++LSP Y HSPA  L +   HG+ L++K ++
Sbjct: 507 VALSPEYVHSPAHKLIVEAEHGVRLVLKKVR 525

BLAST of Spg002423 vs. ExPASy TrEMBL
Match: A0A6J1G224 (cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.8e-254
Identity = 433/512 (84.57%), Postives = 468/512 (91.41%), Query Frame = 0

Query: 34  VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
           V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMK+  KA+AAING + 
Sbjct: 14  VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73

Query: 94  ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
           +  S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74  SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133

Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
           GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193

Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
           SSLVNSA  EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253

Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
           PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++  SSSDLLSRLIE +++   GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313

Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
           KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373

Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
            MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433

Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
           WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493

Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

BLAST of Spg002423 vs. ExPASy TrEMBL
Match: A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 2.0e-252
Identity = 434/512 (84.77%), Postives = 467/512 (91.21%), Query Frame = 0

Query: 34  VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
           V IMVLFVKVV+SWW+LP+LAHQKLKRNGF GP P+FPLGNIAEMK+  KA+AAING S 
Sbjct: 14  VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPTFPLGNIAEMKRIRKASAAINGDSH 73

Query: 94  ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
           +  S+ VSHDIHS VFPHFAQWQASYGKKFVYWLGTEPFLYIADP F+K+IS AVM KGW
Sbjct: 74  SFPSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVMAKGW 133

Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
           GKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMTKFMVESTT+MLN+W
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNEW 193

Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
           SSLVNSA  EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNSTYVGV 253

Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
           PFS LF CP RTLAAKRLGA IDRLFLS++SERK S+  +SSSDLLSRLIE Q++    G
Sbjct: 254 PFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSDLLSRLIEGQKSTGDVG 313

Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
           KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373

Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
            MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMHRDPDL 433

Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
           WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLILSRFS 493

Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

BLAST of Spg002423 vs. ExPASy TrEMBL
Match: A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 8.5e-251
Identity = 435/523 (83.17%), Postives = 475/523 (90.82%), Query Frame = 0

Query: 33  RVSIMVLFVKVVISWWILPILAHQKLKRNGFE-GPSPSFPLGNIAEMKKTMK------AA 92
           RVS++VL V+VV SWWILP+LAH+KLKRNGF  GP P FPLGNI E+K TMK      AA
Sbjct: 13  RVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 72

Query: 93  AAINGGSFA-SSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI 152
           AAINGG+ A SSS+N+SHDIHS VFP+FAQWQ SYGKKFVYWLGTEPFLYIA+PQFVK +
Sbjct: 73  AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 132

Query: 153 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVE 212
           SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMT+ MV 
Sbjct: 133 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVG 192

Query: 213 STTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTL 272
           STT+ML++W+  ++S  PE+DVEREITATAGDIIA+ASFGI +  GRDAFN LR LQFTL
Sbjct: 193 STTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTL 252

Query: 273 FQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIE 332
           F+NS+YVGVPFS +FL P RTL AKRLG+EIDRLFLS+ISERKQS + +S+SDLLSRLIE
Sbjct: 253 FRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD-ASASDLLSRLIE 312

Query: 333 SQRNGS--GGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEI 392
           SQRN S    GK  LTTRELVDECKTFFFGGHETTALAISWT+LLLATRPEWQ ILREEI
Sbjct: 313 SQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEI 372

Query: 393 KEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWI 452
           KEVIGD+EIDFAMLS L+KMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD TIPKGTNIWI
Sbjct: 373 KEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWI 432

Query: 453 DVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYK 512
           D+V+MHHDP+LWG+DVNEFKPERFEKDSIHGGC HKMGYLPFGFGGRMCIGRNLSSMEYK
Sbjct: 433 DIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYK 492

Query: 513 IVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           IVLTLI+SRFSLS+SPSYSHSPAILLSLRPAHGLPLI+KPL P
Sbjct: 493 IVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534

BLAST of Spg002423 vs. ExPASy TrEMBL
Match: A0A1S3AXX2 (cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 1.0e-243
Identity = 422/514 (82.10%), Postives = 458/514 (89.11%), Query Frame = 0

Query: 33  RVSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGS 92
           RVSIMVLF KVVIS WILPILAH+KLKRNGFEGPSPSFPLGNI EMKK MKAAAA    +
Sbjct: 16  RVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVT 75

Query: 93  FASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKG 152
             SSSNN+SHDIHS +FPHFAQWQ SYGKKFVYWLGTEPFLYIADPQ VKKISEAV+GK 
Sbjct: 76  NGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKS 135

Query: 153 WGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNK 212
           WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKAMTKFMVESTT+ML +
Sbjct: 136 WGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEE 195

Query: 213 WSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNSLRALQFTLFQNSTYV 272
           W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+LRALQ TLF+NS+YV
Sbjct: 196 WRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNSSYV 255

Query: 273 GVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSG 332
           GVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S   S  SDLLSRLI S+  G  
Sbjct: 256 GVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGE 315

Query: 333 GGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEI 392
                L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ ILREEIKEV+GDK+I
Sbjct: 316 A----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDI 375

Query: 393 DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDP 452
           DF+MLS L+KMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPKGTNIWIDVV+MHHDP
Sbjct: 376 DFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDP 435

Query: 453 TLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSR 512
           TLWG+DVN+F P RF+ D+IHGGC HKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILS 
Sbjct: 436 TLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILST 495

Query: 513 FSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 496 FSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 525

BLAST of Spg002423 vs. ExPASy TrEMBL
Match: A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 9.2e-221
Identity = 382/469 (81.45%), Postives = 417/469 (88.91%), Query Frame = 0

Query: 78  MKKTMKAAAAINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIAD 137
           MKK MKAAAA    +  SSSNN+SHDIHS +FPHFAQWQ SYGKKFVYWLGTEPFLYIAD
Sbjct: 1   MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 60

Query: 138 PQFVKKISEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKA 197
           PQ VKKISEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKA
Sbjct: 61  PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 120

Query: 198 MTKFMVESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNS 257
           MTKFMVESTT+ML +W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180

Query: 258 LRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS 317
           LRALQ TLF+NS+YVGVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S   S  S
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240

Query: 318 DLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQN 377
           DLLSRLI S+  G       L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ 
Sbjct: 241 DLLSRLIGSEYTGGEA----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQR 300

Query: 378 ILREEIKEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPK 437
           ILREEIKEV+GDK+IDF+MLS L+KMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPK
Sbjct: 301 ILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPK 360

Query: 438 GTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNL 497
           GTNIWIDVV+MHHDPTLWG+DVN+F P RF+ D+IHGGCNHKMGYLPFGFGGRMCIGRNL
Sbjct: 361 GTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNL 420

Query: 498 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
           SSMEYKIVLTLILS FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 421 SSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

BLAST of Spg002423 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 634.8 bits (1636), Expect = 6.4e-182
Identity = 303/512 (59.18%), Postives = 385/512 (75.20%), Query Frame = 0

Query: 34  VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
           V I ++F+K+ +  WI P+ A +KL+ NGF GP+PSFP GN+ +MKK   A+  ++    
Sbjct: 19  VVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDN--- 78

Query: 94  ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
           + SS  ++HDIHSI  PHFA+WQ  YGK FVYWLG EPF+Y+ADP+F+  +S+ V+GK W
Sbjct: 79  SKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSW 138

Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
           GKP VF+ DREPMFG GLVM EGDDW RHRHI+TPAF P NLK MT  MVES + ML++W
Sbjct: 139 GKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRW 198

Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
              +NS  PE D+E EI  TAG+IIA+ SFG++   G     +LRA+QF LF ++ YVGV
Sbjct: 199 GIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRYVGV 258

Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQS--EENSSSSDLLSRLIESQRNGSG 333
           PFS + L   +T+ AK LG EID L LS I++RK S  E +    DLL  L+++ + G+ 
Sbjct: 259 PFSNI-LSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLGMLLKADQKGN- 318

Query: 334 GGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEI 393
                 T +ELVDECKTFFF GHETTALA++WT +LLA  PEWQ+ +REEI+EVIGD +I
Sbjct: 319 -----FTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREVIGDSKI 378

Query: 394 DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDP 453
           ++  L+GL+KM WV NEVLRLYP APN QRQA+ DI+V    IP GTNIWIDVV+MHHD 
Sbjct: 379 EYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDV 438

Query: 454 TLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSR 513
            LWG+DVNEFKPERF+  ++HGGC +KMGY+PFGFGGRMCIGRNL++MEYKIVL+L+LSR
Sbjct: 439 ELWGDDVNEFKPERFD-GNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSR 498

Query: 514 FSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
           F +S+SP Y HSP  +LSLRP +GLPLI++PL
Sbjct: 499 FEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

BLAST of Spg002423 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 330.1 bits (845), Expect = 3.4e-90
Identity = 185/518 (35.71%), Postives = 283/518 (54.63%), Query Frame = 0

Query: 34  VSIMVLFVKV----VISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAIN 93
           V +M L ++V    +  +++ P    + ++R G  GP P    GNI ++ K +  +A   
Sbjct: 12  VIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSA--- 71

Query: 94  GGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI---SE 153
               ++  +++ H+I   + PH+  W   YGK+F+ W GTEP L + + + +K++     
Sbjct: 72  ----SNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHN 131

Query: 154 AVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVEST 213
            V GK W    + +   +   G GL+M  G+ W   RH+  PAF    LK   K MVE T
Sbjct: 132 PVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 191

Query: 214 TQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQ 273
             M  +   L      E+++  E+     DII++  FG S   G++ F+ L  LQ    Q
Sbjct: 192 KMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 251

Query: 274 NSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQ 333
            + ++  P S  FL        K L  E++RL + +I  RK S E   SS     L+   
Sbjct: 252 ATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLL 311

Query: 334 RNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVI 393
            N     K  L  + ++DECKTFFF GHETT+L ++WTL+LLA  P WQ+ +R+E+++V 
Sbjct: 312 LNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVC 371

Query: 394 GDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 453
           G   +     LS L  +  V NE LRLYP A  + R A  DIK+GD+ IPKG +IWI V+
Sbjct: 372 GQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVL 431

Query: 454 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 513
           ++HH   LWGED NEF PERF   S      H   ++PF  G R CIG+  + ME KI+L
Sbjct: 432 AIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFAMMEAKIIL 491

Query: 514 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
            +++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 492 AMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of Spg002423 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 327.4 bits (838), Expect = 2.2e-89
Identity = 184/527 (34.91%), Postives = 284/527 (53.89%), Query Frame = 0

Query: 35  SIMVLFVKVVI--------SWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAA 94
           S++V+FV  ++         +W+ P    + +++ G  GP P    GNI E+       +
Sbjct: 8   SLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI-------S 67

Query: 95  AINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI-- 154
           A+   S +   +++ HDI   + PH+  W   YGK+F+ W GT+P L + + + +K++  
Sbjct: 68  AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 127

Query: 155 -SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMV 214
               V G+ W +    +N      G GL+M  G DW   RH+  PAF    LK   + MV
Sbjct: 128 KHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMV 187

Query: 215 ESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFT 274
           E T++++ +    V     E+++  E+     DII++  FG S   G++ FN L  LQ  
Sbjct: 188 ECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRR 247

Query: 275 LFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS----DLL 334
             Q + ++  P S  FL        K L  E++RL + +I  R+   E   SS    DLL
Sbjct: 248 CAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 307

Query: 335 SRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILR 394
             L+                + ++DECKTFFF GHETTAL ++WT +LLA  P WQ  +R
Sbjct: 308 GLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVR 367

Query: 395 EEIKEVIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGT 454
           EE++EV G   +     LS L  +  V NE LRLYP A  + R A  D+K+GD+TIPKG 
Sbjct: 368 EEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGL 427

Query: 455 NIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMCIGRNL 514
           +IWI V+++HH   LWG+D N+F PERF      GG     G  ++PF  G R CIG+  
Sbjct: 428 SIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCIGQQF 487

Query: 515 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
           + ME KI+L  ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 488 ALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Spg002423 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 306.6 bits (784), Expect = 4.0e-83
Identity = 172/515 (33.40%), Postives = 273/515 (53.01%), Query Frame = 0

Query: 39  LFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSSN 98
           ++ + V+  W +     + LK  G +GP PS   GN++EM++    A         S  N
Sbjct: 27  VYGRAVVEQWRM----RRSLKLQGVKGPPPSIFNGNVSEMQRIQSEA------KHCSGDN 86

Query: 99  NVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAV 158
            +SHD  S +FPHF  W+  YG+ + Y  G +  LYI  P+ VK++S+       G+   
Sbjct: 87  IISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQ-TNTLNLGRITH 146

Query: 159 FRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVN 218
                 P+ G+G++ + G  W   R I+   F    +K M   MVES   MLNKW  +V 
Sbjct: 147 ITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVK 206

Query: 219 SAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPF 278
                  +I V+ ++   + D+IA+A FG S   G+  F+ +R L   + + S  V   F
Sbjct: 207 RGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS--VLFRF 266

Query: 279 SGLFLCPARTLAAKRLG-AEIDRLFLSV-------ISERKQSEENSSSSDLLSRLIESQR 338
           +G          +K+ G  +ID L + +       + ER+   +++   DL+  ++E   
Sbjct: 267 NGF---TDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAM 326

Query: 339 NGSGGGKMRLTT--RELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEV 398
               G     +   R +VD CK+ +F GH++TA+++SW L+LLA  P WQ  +R+EI   
Sbjct: 327 RSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSS 386

Query: 399 IGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 458
             +   D   +  L+ +  V  E +RLYP AP + R+A  DI++GD+ +PKG  IW  + 
Sbjct: 387 CKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIP 446

Query: 459 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 518
           ++H DP +WG D N+FKPERF  + I   C +   Y+PFG G R C+G+N   ME K+++
Sbjct: 447 ALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLV 506

Query: 519 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 541
           +LI+S+FS +LSP+Y HSP+  L + P HG+ + V
Sbjct: 507 SLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVIRV 524

BLAST of Spg002423 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 305.1 bits (780), Expect = 1.2e-82
Identity = 167/511 (32.68%), Postives = 272/511 (53.23%), Query Frame = 0

Query: 39  LFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSSN 98
           ++ KV+   W +     +KL   G +GP PS   GN+ EM+K    +  ++     S  N
Sbjct: 27  VYGKVMAEQWRM----RRKLTMQGVKGPPPSLFRGNVPEMQKIQ--SQIMSNSKHYSGDN 86

Query: 99  NVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAV 158
            ++HD  S +FP+   W+  YG+ + Y  G +  LY+  P+ VK++++A      GK + 
Sbjct: 87  IIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQA-NTLNLGKVSY 146

Query: 159 FRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVN 218
                + + G G++ + G  W   R I+ P F    +K M   +VES   ML+KW  ++ 
Sbjct: 147 VTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMK 206

Query: 219 SAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV-P 278
                  +I V+ ++ A + D+I++A FG S   G++ F+ LR LQ  +  N+    +  
Sbjct: 207 REGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNG 266

Query: 279 FSGLFLCPARTLAAK--RLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGG 338
           F+ +     +    K   L   I+ L    + ER++        DL+  ++E  R+   G
Sbjct: 267 FTDVVFGTKKHGNGKIDELERHIESLIWETVKERERECVGDHKKDLMQLILEGARSSCDG 326

Query: 339 G---KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDK 398
               K +     +VD CK+ +F GHET+A+A+SW L+LLA  P WQ  +R+E+     + 
Sbjct: 327 NLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEVFLHCKNG 386

Query: 399 EIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHH 458
             D   +S L+ +  V  E LRLYP A  + R+A  D K+G++ +PKG  IW  + ++H 
Sbjct: 387 IPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHR 446

Query: 459 DPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLIL 518
           DP +WG D NEF PERF  + +   C H   ++PFG G R+C+G+N   ME K++++LI+
Sbjct: 447 DPEIWGADANEFNPERF-SEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIV 506

Query: 519 SRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 541
           SRFS +LSP+Y HSP   + + P HG+ + V
Sbjct: 507 SRFSFTLSPTYQHSPVFRMLVEPQHGVVIRV 529