Spg002406 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg002406 vs. NCBI nr
Match: XP_022140910.1 (U-box domain-containing protein 32 isoform X1 [Momordica charantia])

HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 650/885 (73.45%), Postives = 704/885 (79.55%), Query Frame = 0

Query: 10  GTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVAQ 69
           GTAEEIGQ+L FDVE TIFVAVG +VD+TK+TL+WAV NF GKNFCLLHVH P       
Sbjct: 3   GTAEEIGQQLVFDVEHTIFVAVGTNVDETKTTLIWAVHNFAGKNFCLLHVHPPP------ 62

Query: 70  SEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKAL 129
                                                                       
Sbjct: 63  ------------------------------------------------------------ 122

Query: 130 ISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQA 189
                       G  + NE PSSNTLKE +AKAFEEP RQKP E LNQYV ILAKLGVQA
Sbjct: 123 -----------PGVGLRNEGPSSNTLKEQIAKAFEEPGRQKPFEPLNQYVAILAKLGVQA 182

Query: 190 QKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVC 249
           QKVW E NSVERGIVEIIAQYSIKWLVMGLDAE  N         +R TGLKS KAF+VC
Sbjct: 183 QKVWIETNSVERGIVEIIAQYSIKWLVMGLDAERYNL--------KRSTGLKSNKAFFVC 242

Query: 250 QQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLED 309
           +QAP+CCHIW VC G LIYSREGRMGRF+P LLQN +I  +QSN+L+PESVT +L+FL  
Sbjct: 243 EQAPMCCHIWLVCRGHLIYSREGRMGRFHPPLLQNLDIGTNQSNNLKPESVTCQLSFL-- 302

Query: 310 SRITLLQNFADAQEKEDAFDRI-SRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVK-GG 369
                     D QEKE+AFD + SRFRS+GLVDPKCSNNG++ TSRT LLLKNEGVK  G
Sbjct: 303 ----------DTQEKENAFDGVLSRFRSQGLVDPKCSNNGILGTSRTILLLKNEGVKERG 362

Query: 370 QPVSQSRGLQEEAVLNVKSIKEFEGVRV--KEDAMGANFENKSLVSSCMEEVKKRKEMEE 429
           +PV Q  GLQ EA++NVK IKEF GV+   +EDA GA F+ KS+ SSCMEEVK+ KEMEE
Sbjct: 363 RPVIQGPGLQ-EAMINVKPIKEFAGVKAWEEEDAAGAKFKAKSIESSCMEEVKEGKEMEE 422

Query: 430 ILVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLIS 489
           +L REK E+E+MNKE DELLKELHDVQEQ  LL RKASEY+CDV+ LE+K+F AVDLL+S
Sbjct: 423 LLEREKLEVEKMNKERDELLKELHDVQEQTLLLGRKASEYQCDVKKLEEKLFAAVDLLVS 482

Query: 490 FKEKRDKLQIEHEGAMNKLRQLKNMVKREPTC--SVEMPTFSFMEIIEATRNFDPSWKIG 549
           FKEKRDKLQIEHEGAMNKLRQLKNMVK EP    S EMPTFSFMEIIEATRNFDPSWKIG
Sbjct: 483 FKEKRDKLQIEHEGAMNKLRQLKNMVKGEPPYFRSAEMPTFSFMEIIEATRNFDPSWKIG 542

Query: 550 EGRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRL 609
           EGRHGSVYKG LRHMDVA+KM PSYGSHSQS+FQYEVEVLSRVRHPNLVSIIGACPESRL
Sbjct: 543 EGRHGSVYKGLLRHMDVAIKMFPSYGSHSQSKFQYEVEVLSRVRHPNLVSIIGACPESRL 602

Query: 610 IVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKI 669
           IVYE+L NSSLE  LACKDHNRPLPWQIRIRVAADICSALIFLHYS+PCIVHGDIKPSKI
Sbjct: 603 IVYENLKNSSLEDQLACKDHNRPLPWQIRIRVAADICSALIFLHYSKPCIVHGDIKPSKI 662

Query: 670 LLDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYS 729
           LLDANF+AKLGGLGISRLIP+EE AF+S  AY+MSKENNPYIDPEYL+TGRFTPESD+YS
Sbjct: 663 LLDANFIAKLGGLGISRLIPREENAFNSAFAYDMSKENNPYIDPEYLQTGRFTPESDIYS 722

Query: 730 LGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKE 789
            GVT LRILTGRAPSGIVKDVKCALEN+KI  +LDSSAGDWPHDLAEQLALVALRCCE++
Sbjct: 723 FGVTLLRILTGRAPSGIVKDVKCALENNKIGAILDSSAGDWPHDLAEQLALVALRCCERD 782

Query: 790 KSDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTY 849
           KS+R DLVSELWSVLEPMRSIAS SFSS KKHSRVPA FTCPIFQEIMKDPLIAADGFTY
Sbjct: 783 KSNRLDLVSELWSVLEPMRSIASTSFSSMKKHSRVPAQFTCPIFQEIMKDPLIAADGFTY 789

Query: 850 EADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           EADAIRGWF SGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 843 EADAIRGWFGSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 789

BLAST of Spg002406 vs. NCBI nr
Match: XP_038892802.1 (U-box domain-containing protein 32-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 649/884 (73.42%), Postives = 701/884 (79.30%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MG+A+ IGQ LQFDVE+TIFVAVG DVDD ++TL+WAVQNF GK+FCLLHVH        
Sbjct: 1   MGSADVIGQLLQFDVEDTIFVAVGTDVDDAEATLIWAVQNFTGKSFCLLHVHH------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     LAP+              
Sbjct: 61  ------------------------------------------LAPK-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                             NEEPSS TLKEH+ KAFEEPERQKP ELLNQYVLILAKLGVQ
Sbjct: 121 ------------------NEEPSSYTLKEHIDKAFEEPERQKPFELLNQYVLILAKLGVQ 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYV 248
           AQKVW E N+VERGIVEIIAQY IKWLVMGLD E         +  +R  GLKSKKAFYV
Sbjct: 181 AQKVWIETNNVERGIVEIIAQYGIKWLVMGLDTE--------RYNMKRSIGLKSKKAFYV 240

Query: 249 CQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLE 308
            QQAPICC IWFVC GRLIYSR+GRMGRFNPL L+N EI I+ +N LRPESVT K     
Sbjct: 241 SQQAPICCDIWFVCRGRLIYSRQGRMGRFNPLPLENLEIGINHTNRLRPESVTCK----- 300

Query: 309 DSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQ 368
                   NFADAQEKE+A DRISRFR + LVD  CSNNG + TSRT LLLKNEGVKGG 
Sbjct: 301 --------NFADAQEKENACDRISRFRYQDLVDKNCSNNGDLGTSRTTLLLKNEGVKGGH 360

Query: 369 PVSQSRGLQEEAVLNVKSIKEFEGVR--VKEDAMGANFENKSLVSSCMEEVKKRKEMEEI 428
           PVSQS GLQEE ++NVKSI++FE V+  V+ED +GA  + KS  SSCMEEVKKR+EMEE+
Sbjct: 361 PVSQS-GLQEEGLMNVKSIQDFEEVKAWVEEDVVGAELKAKSSESSCMEEVKKRREMEEL 420

Query: 429 LVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISF 488
           L REKKE+ERMNKEHDELLKEL  VQEQKSLLERKASEY+C+VE+LEKKMF A+DLL+SF
Sbjct: 421 LEREKKEVERMNKEHDELLKELQHVQEQKSLLERKASEYQCEVEELEKKMFAAIDLLVSF 480

Query: 489 KEKRDKLQIEHEGAMNKLRQLKNMVKREPT--CSVEMPTFSFMEIIEATRNFDPSWKIGE 548
           KEKRDKLQIEHEGA++KLR+LKN+VK+EP+   S EMPTFSFMEIIEATRNFDPSWKIGE
Sbjct: 481 KEKRDKLQIEHEGAVDKLRKLKNIVKKEPSRYRSAEMPTFSFMEIIEATRNFDPSWKIGE 540

Query: 549 GRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLI 608
           GRHG+VYKG LRHMDVALKM PSYGSHSQS FQYEVEVLSRVRHPNLVSIIGACPESRLI
Sbjct: 541 GRHGNVYKGLLRHMDVALKMFPSYGSHSQSTFQYEVEVLSRVRHPNLVSIIGACPESRLI 600

Query: 609 VYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKIL 668
           VYE+L N SLE HL+ K+HNRPL WQIRIR+AADICSALIFLHYS PCIVHGDIKPSKIL
Sbjct: 601 VYENLKNGSLEDHLSYKNHNRPLSWQIRIRIAADICSALIFLHYSNPCIVHGDIKPSKIL 660

Query: 669 LDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYSL 728
           LDANF+AKLGGLGISRLIPQEEK     S  NMSKE+NPYIDPEYLETGRF PESDVYSL
Sbjct: 661 LDANFIAKLGGLGISRLIPQEEKV---ASVCNMSKEDNPYIDPEYLETGRFAPESDVYSL 720

Query: 729 GVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKEK 788
           GV  LRILT RAP GIVKDVKCALEND+I  +LDSSAGDWPHDLAEQLALVALRCCEKEK
Sbjct: 721 GVILLRILTARAPPGIVKDVKCALENDEIGAILDSSAGDWPHDLAEQLALVALRCCEKEK 778

Query: 789 SDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYE 848
            DRPDLVSELWSVLEPMRSIASAS SS KKH+RVPAHFTCPIFQEIMKDPLIAADGFTYE
Sbjct: 781 LDRPDLVSELWSVLEPMRSIASASCSSLKKHARVPAHFTCPIFQEIMKDPLIAADGFTYE 778

Query: 849 ADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           ADAIRGWFKSGH+TSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 ADAIRGWFKSGHNTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 778

BLAST of Spg002406 vs. NCBI nr
Match: XP_038892784.1 (U-box domain-containing protein 32-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 651/895 (72.74%), Postives = 705/895 (78.77%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MG+A+ IGQ LQFDVE+TIFVAVG DVDD ++TL+WAVQNF GK+FCLLHVH        
Sbjct: 1   MGSADVIGQLLQFDVEDTIFVAVGTDVDDAEATLIWAVQNFTGKSFCLLHVHH------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     LAP+              
Sbjct: 61  ------------------------------------------LAPK-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                             NEEPSS TLKEH+ KAFEEPERQKP ELLNQYVLILAKLGVQ
Sbjct: 121 ------------------NEEPSSYTLKEHIDKAFEEPERQKPFELLNQYVLILAKLGVQ 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMH----FR-------ERRP 248
           AQKVW E N+VERGIVEIIAQY IKWLVMGLD E  N     +    F+       ++R 
Sbjct: 181 AQKVWIETNNVERGIVEIIAQYGIKWLVMGLDTERYNMNSTTNGGGRFKHPTFWCLDQRS 240

Query: 249 TGLKSKKAFYVCQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRP 308
            GLKSKKAFYV QQAPICC IWFVC GRLIYSR+GRMGRFNPL L+N EI I+ +N LRP
Sbjct: 241 IGLKSKKAFYVSQQAPICCDIWFVCRGRLIYSRQGRMGRFNPLPLENLEIGINHTNRLRP 300

Query: 309 ESVTFKLNFLEDSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRL 368
           ESVT K             NFADAQEKE+A DRISRFR + LVD  CSNNG + TSRT L
Sbjct: 301 ESVTCK-------------NFADAQEKENACDRISRFRYQDLVDKNCSNNGDLGTSRTTL 360

Query: 369 LLKNEGVKGGQPVSQSRGLQEEAVLNVKSIKEFEGVR--VKEDAMGANFENKSLVSSCME 428
           LLKNEGVKGG PVSQS GLQEE ++NVKSI++FE V+  V+ED +GA  + KS  SSCME
Sbjct: 361 LLKNEGVKGGHPVSQS-GLQEEGLMNVKSIQDFEEVKAWVEEDVVGAELKAKSSESSCME 420

Query: 429 EVKKRKEMEEILVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKK 488
           EVKKR+EMEE+L REKKE+ERMNKEHDELLKEL  VQEQKSLLERKASEY+C+VE+LEKK
Sbjct: 421 EVKKRREMEELLEREKKEVERMNKEHDELLKELQHVQEQKSLLERKASEYQCEVEELEKK 480

Query: 489 MFEAVDLLISFKEKRDKLQIEHEGAMNKLRQLKNMVKREPT--CSVEMPTFSFMEIIEAT 548
           MF A+DLL+SFKEKRDKLQIEHEGA++KLR+LKN+VK+EP+   S EMPTFSFMEIIEAT
Sbjct: 481 MFAAIDLLVSFKEKRDKLQIEHEGAVDKLRKLKNIVKKEPSRYRSAEMPTFSFMEIIEAT 540

Query: 549 RNFDPSWKIGEGRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVS 608
           RNFDPSWKIGEGRHG+VYKG LRHMDVALKM PSYGSHSQS FQYEVEVLSRVRHPNLVS
Sbjct: 541 RNFDPSWKIGEGRHGNVYKGLLRHMDVALKMFPSYGSHSQSTFQYEVEVLSRVRHPNLVS 600

Query: 609 IIGACPESRLIVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCI 668
           IIGACPESRLIVYE+L N SLE HL+ K+HNRPL WQIRIR+AADICSALIFLHYS PCI
Sbjct: 601 IIGACPESRLIVYENLKNGSLEDHLSYKNHNRPLSWQIRIRIAADICSALIFLHYSNPCI 660

Query: 669 VHGDIKPSKILLDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETG 728
           VHGDIKPSKILLDANF+AKLGGLGISRLIPQEEK     S  NMSKE+NPYIDPEYLETG
Sbjct: 661 VHGDIKPSKILLDANFIAKLGGLGISRLIPQEEKV---ASVCNMSKEDNPYIDPEYLETG 720

Query: 729 RFTPESDVYSLGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLA 788
           RF PESDVYSLGV  LRILT RAP GIVKDVKCALEND+I  +LDSSAGDWPHDLAEQLA
Sbjct: 721 RFAPESDVYSLGVILLRILTARAPPGIVKDVKCALENDEIGAILDSSAGDWPHDLAEQLA 780

Query: 789 LVALRCCEKEKSDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKD 848
           LVALRCCEKEK DRPDLVSELWSVLEPMRSIASAS SS KKH+RVPAHFTCPIFQEIMKD
Sbjct: 781 LVALRCCEKEKLDRPDLVSELWSVLEPMRSIASASCSSLKKHARVPAHFTCPIFQEIMKD 797

Query: 849 PLIAADGFTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           PLIAADGFTYEADAIRGWFKSGH+TSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 PLIAADGFTYEADAIRGWFKSGHNTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 797

BLAST of Spg002406 vs. NCBI nr
Match: XP_038892792.1 (U-box domain-containing protein 32-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 646/895 (72.18%), Postives = 701/895 (78.32%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MG+A+ IGQ LQFDVE+TIFVAVG DVDD ++TL+WAVQNF GK+FCLLHVH        
Sbjct: 1   MGSADVIGQLLQFDVEDTIFVAVGTDVDDAEATLIWAVQNFTGKSFCLLHVHH------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     LAP+              
Sbjct: 61  ------------------------------------------LAPK-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                             NEEPSS TLKEH+ KAFEEPERQKP ELLNQYVLILAKLGVQ
Sbjct: 121 ------------------NEEPSSYTLKEHIDKAFEEPERQKPFELLNQYVLILAKLGVQ 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMH----FR-------ERRP 248
           AQKVW E N+VERGIVEIIAQY IKWLVMGLD E  N     +    F+       ++R 
Sbjct: 181 AQKVWIETNNVERGIVEIIAQYGIKWLVMGLDTERYNMNSTTNGGGRFKHPTFWCLDQRS 240

Query: 249 TGLKSKKAFYVCQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRP 308
            GLKSKKAFYV QQAPICC IWFVC GRLIYSR+GRMGRFNPL L+N EI I+ +N LRP
Sbjct: 241 IGLKSKKAFYVSQQAPICCDIWFVCRGRLIYSRQGRMGRFNPLPLENLEIGINHTNRLRP 300

Query: 309 ESVTFKLNFLEDSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRL 368
           ESVT+                  AQEKE+A DRISRFR + LVD  CSNNG + TSRT L
Sbjct: 301 ESVTY------------------AQEKENACDRISRFRYQDLVDKNCSNNGDLGTSRTTL 360

Query: 369 LLKNEGVKGGQPVSQSRGLQEEAVLNVKSIKEFEGVR--VKEDAMGANFENKSLVSSCME 428
           LLKNEGVKGG PVSQS GLQEE ++NVKSI++FE V+  V+ED +GA  + KS  SSCME
Sbjct: 361 LLKNEGVKGGHPVSQS-GLQEEGLMNVKSIQDFEEVKAWVEEDVVGAELKAKSSESSCME 420

Query: 429 EVKKRKEMEEILVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKK 488
           EVKKR+EMEE+L REKKE+ERMNKEHDELLKEL  VQEQKSLLERKASEY+C+VE+LEKK
Sbjct: 421 EVKKRREMEELLEREKKEVERMNKEHDELLKELQHVQEQKSLLERKASEYQCEVEELEKK 480

Query: 489 MFEAVDLLISFKEKRDKLQIEHEGAMNKLRQLKNMVKREPT--CSVEMPTFSFMEIIEAT 548
           MF A+DLL+SFKEKRDKLQIEHEGA++KLR+LKN+VK+EP+   S EMPTFSFMEIIEAT
Sbjct: 481 MFAAIDLLVSFKEKRDKLQIEHEGAVDKLRKLKNIVKKEPSRYRSAEMPTFSFMEIIEAT 540

Query: 549 RNFDPSWKIGEGRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVS 608
           RNFDPSWKIGEGRHG+VYKG LRHMDVALKM PSYGSHSQS FQYEVEVLSRVRHPNLVS
Sbjct: 541 RNFDPSWKIGEGRHGNVYKGLLRHMDVALKMFPSYGSHSQSTFQYEVEVLSRVRHPNLVS 600

Query: 609 IIGACPESRLIVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCI 668
           IIGACPESRLIVYE+L N SLE HL+ K+HNRPL WQIRIR+AADICSALIFLHYS PCI
Sbjct: 601 IIGACPESRLIVYENLKNGSLEDHLSYKNHNRPLSWQIRIRIAADICSALIFLHYSNPCI 660

Query: 669 VHGDIKPSKILLDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETG 728
           VHGDIKPSKILLDANF+AKLGGLGISRLIPQEEK     S  NMSKE+NPYIDPEYLETG
Sbjct: 661 VHGDIKPSKILLDANFIAKLGGLGISRLIPQEEKV---ASVCNMSKEDNPYIDPEYLETG 720

Query: 729 RFTPESDVYSLGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLA 788
           RF PESDVYSLGV  LRILT RAP GIVKDVKCALEND+I  +LDSSAGDWPHDLAEQLA
Sbjct: 721 RFAPESDVYSLGVILLRILTARAPPGIVKDVKCALENDEIGAILDSSAGDWPHDLAEQLA 780

Query: 789 LVALRCCEKEKSDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKD 848
           LVALRCCEKEK DRPDLVSELWSVLEPMRSIASAS SS KKH+RVPAHFTCPIFQEIMKD
Sbjct: 781 LVALRCCEKEKLDRPDLVSELWSVLEPMRSIASASCSSLKKHARVPAHFTCPIFQEIMKD 792

Query: 849 PLIAADGFTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           PLIAADGFTYEADAIRGWFKSGH+TSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 PLIAADGFTYEADAIRGWFKSGHNTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 792

BLAST of Spg002406 vs. NCBI nr
Match: XP_011651168.1 (U-box domain-containing protein 32 isoform X1 [Cucumis sativus] >KAE8650460.1 hypothetical protein Csa_011578 [Cucumis sativus])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 639/884 (72.29%), Postives = 695/884 (78.62%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MG+A EIG   QFDVE+TIFVAVG DVDD K+TL+WAVQNF GK+FCLLHVH        
Sbjct: 1   MGSAVEIGLPFQFDVEDTIFVAVGTDVDDAKATLIWAVQNFAGKSFCLLHVHH------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     L P+              
Sbjct: 61  ------------------------------------------LPPK-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                             NEEPSS TLKEHVA+AFEE ERQKP ELLNQYVLILAKLGVQ
Sbjct: 121 ------------------NEEPSSYTLKEHVAQAFEELERQKPFELLNQYVLILAKLGVQ 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYV 248
           AQKVW E N+VERGIVEII+QYSIKWLVMGLD E  N         +R TGLKSKKAFYV
Sbjct: 181 AQKVWIETNNVERGIVEIISQYSIKWLVMGLDTEGYNM--------KRSTGLKSKKAFYV 240

Query: 249 CQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLE 308
            QQAPICCHIWFVC GRLIYSRE RMGR     L+NSEI I+ +NHLRPESVT K     
Sbjct: 241 SQQAPICCHIWFVCRGRLIYSREARMGR-----LKNSEIGINYTNHLRPESVTCK----- 300

Query: 309 DSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQ 368
                   NFADAQEKE A DRISRFR +GLVD K SNNG + TSRT LLLKNEGVK G 
Sbjct: 301 --------NFADAQEKECAGDRISRFRYQGLVDQKLSNNGNLGTSRTTLLLKNEGVKEGH 360

Query: 369 PVSQSRGLQEEAVLNVKSIKEFEGVR--VKEDAMGANFENKSLVSSCMEEVKKRKEMEEI 428
           PVSQS GLQ EA +NVKSIK+FEGV+  V++DA+GA F+ + L +SCMEEVKKRKEMEE+
Sbjct: 361 PVSQS-GLQ-EASMNVKSIKDFEGVKAWVEKDAVGAEFKAELLENSCMEEVKKRKEMEEL 420

Query: 429 LVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISF 488
           L +EK+E+ER+NKE  ELLKEL  V EQKS+L+RKASEY+CD+E+LEKKMF AVDLL+SF
Sbjct: 421 LEKEKREVERINKERAELLKELQHVDEQKSVLDRKASEYQCDMEELEKKMFAAVDLLVSF 480

Query: 489 KEKRDKLQIEHEGAMNKLRQLKNMVKREPT--CSVEMPTFSFMEIIEATRNFDPSWKIGE 548
           K+KRDKL IE EGAM+KLR+LKN+VKREP+   + EMP FSF+EIIEATRNFDPSWKIGE
Sbjct: 481 KDKRDKLLIEREGAMDKLRKLKNIVKREPSRYRNAEMPMFSFVEIIEATRNFDPSWKIGE 540

Query: 549 GRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLI 608
           GRHGSVYKG LRHMDVALKM PSYGSHSQS FQYEVEVLSRVRHPNLVSIIGACPESRLI
Sbjct: 541 GRHGSVYKGLLRHMDVALKMFPSYGSHSQSAFQYEVEVLSRVRHPNLVSIIGACPESRLI 600

Query: 609 VYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKIL 668
           VYE+L N SLE HLACK+HN PLPWQ RIR+AADICSALIFLHYS+PCIVHGDIKPSKIL
Sbjct: 601 VYENLKNGSLEDHLACKNHNCPLPWQTRIRIAADICSALIFLHYSDPCIVHGDIKPSKIL 660

Query: 669 LDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYSL 728
           LD NF+AKLGGLGISRLIPQEEKAF+S S  N+SKENNPYIDPE LETG+FTPESDVYS+
Sbjct: 661 LDTNFIAKLGGLGISRLIPQEEKAFNSASMCNISKENNPYIDPECLETGKFTPESDVYSI 720

Query: 729 GVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKEK 788
           GV  LRILTGR P GIV+DVKCA+END I  VLDSSAGDWPHDLAEQLALVALRCC+KEK
Sbjct: 721 GVILLRILTGRTPPGIVEDVKCAIENDNIVVVLDSSAGDWPHDLAEQLALVALRCCKKEK 774

Query: 789 SDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYE 848
            +RPDLVSELW VLEPMRSIASAS SSSKKH RVPAHFTCPIFQEIMKDPLIAADGFTYE
Sbjct: 781 LERPDLVSELWCVLEPMRSIASASCSSSKKH-RVPAHFTCPIFQEIMKDPLIAADGFTYE 774

Query: 849 ADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           ADAIRGWFKSGH+TSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 ADAIRGWFKSGHNTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 774

BLAST of Spg002406 vs. ExPASy Swiss-Prot
Match: Q94A51 (U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 7.3e-164
Identity = 356/919 (38.74%), Postives = 518/919 (56.37%), Query Frame = 0

Query: 16  GQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVAQSEFCSY 75
           G++L  DV+ETIFVAV  DV+ +K+T+LWA +NF GK  CLL+VH+ A            
Sbjct: 6   GEELVLDVDETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTAR----------- 65

Query: 76  GISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKALISYLPL 135
                            + W                                        
Sbjct: 66  ----------------AASW---------------------------------------- 125

Query: 136 NQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQAQKVWTE 195
              + VGG          + K+H  K  E  E+ K  EL+N Y+ +L++  +Q  K+   
Sbjct: 126 THKKLVGG----------SFKKHDVKVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIA 185

Query: 196 MNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVCQQAPIC 255
             ++E  IVE+IA++ IKWLVMG  A SD      H+   + T LKSKKA +VC++AP  
Sbjct: 186 GQNIEECIVELIARHKIKWLVMG--AASDK-----HY-SWKMTDLKSKKAIFVCKKAPDS 245

Query: 256 CHIWFVCSGRLIYSREGRMGRFN-----PLLLQNSEIVIDQSNHLRPESVTFKLNFLEDS 315
           CHIWF+C G LI++R       N     PL+  +S+    +S  L    +  +L +    
Sbjct: 246 CHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQLDSDNETRKSEKLESSYMRRRLRYWR-- 305

Query: 316 RITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQPV 375
             +LL+     Q+ E    ++ R + E    P  S+    S              G +PV
Sbjct: 306 --SLLE-----QDGEKDTGQLEREKVEPRAPPLFSSGSSSSFGEP---------VGPEPV 365

Query: 376 SQSRGLQEEAVLNVKSIKEFE------------------------------GVRVKEDA- 435
           S    L +   LN  +++E E                              G + +EDA 
Sbjct: 366 SPE--LVDSDTLNTSNVEEKEREGDVARKVHRYDKAMHDIGQSDRTVYGEAGKKWEEDAS 425

Query: 436 -MGANFENKSLVSSCMEEVKKRKEMEEILVREKKELERMNKEHDELLKELHDVQEQKSLL 495
              A  + K+L   C++E  +RK +EE+L +EK E++ + ++++  +KEL  VQ +   L
Sbjct: 426 TTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMVIEQNNGFMKELQMVQGRNLKL 485

Query: 496 ERKASEYRCDVEDLEK----KMFEAVDLLISFKEKRDKLQIEHEGAMNKLRQLKNMVKRE 555
           E +  +    ++DLEK    K   A++LL SF++KRD+++I+HE A+ ++  L+ +VK E
Sbjct: 486 ESQMRK----LQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHENAVKEVNALRRLVKGE 545

Query: 556 --PTCSVEMPTFSFMEIIEATRNFDPSWKIGEGRHGSVYKGFLRHMDVALKMLPSYGSHS 615
              +   EM  +SFMEI EAT  FDPSWK+GEG++GSVYKG L+H+ VA+KMLPSYGS +
Sbjct: 546 TGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQHLQVAVKMLPSYGSLN 605

Query: 616 QSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVYEHLTNSSLESHLACKDHNRPLPWQIR 675
              F+  VE+LSRVRHPNLV+++GACPESR ++Y+++ N SLE   + +++   L W+ R
Sbjct: 606 HFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESR 665

Query: 676 IRVAADICSALIFLHYSEPCIVHGDIKPSKILLDANFVAKLGGLGISRLIPQEEKAFSSD 735
           IR+A++ICSAL+FLH + PCI+HG++KPSKILLD+N V K+   GIS+LIP         
Sbjct: 666 IRIASEICSALLFLHSNIPCIIHGNLKPSKILLDSNLVTKINDYGISQLIP--------- 725

Query: 736 SAYNMSKENNPYIDPEYLETGRFTPESDVYSLGVTFLRILTGRAPSGIVKDVKCALENDK 795
              +   +++P++DP Y  +   T ESD+Y+ G+  L++LT R  SGI++DVKCALEND 
Sbjct: 726 --IDGLDKSDPHVDPHYFVSREMTLESDIYAFGIILLQLLTRRPVSGILRDVKCALENDN 785

Query: 796 ISTVLDSSAGDWPHDLAEQLALVALRCCEKEKSDRPDLVSELWSV----LEPMRSIASAS 855
           IS VLD+SAGDWP    ++LA VA+RCC+K   +RPDL   L  +       + S  ++S
Sbjct: 786 ISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDLAVVLRFIDRMKAPEVPSSETSS 804

Query: 856 FSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYEADAIRGWFKSGHDTSPMTNLKLEH 888
           +++     R P+H+ CPIFQE+MKDPLIAADGFTYEA+AIR W  +GHDTSPMTNLK+E 
Sbjct: 846 YANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMED 804

BLAST of Spg002406 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 523.9 bits (1348), Expect = 3.6e-147
Identity = 327/899 (36.37%), Postives = 483/899 (53.73%), Query Frame = 0

Query: 23  VEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVAQSEFCSYGISFFGH 82
           ++E IFVAV   V  +KSTL+WA+QN  GK  CL+HVHQP+                   
Sbjct: 43  IDEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPS------------------- 102

Query: 83  VNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKALISYLPLNQSRCVG 142
                                                             +PL  ++   
Sbjct: 103 ------------------------------------------------QMIPLMGAKFPV 162

Query: 143 GAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQAQKVWTEMNSVERG 202
           GA++ EE           + F E ER+K   +L+ Y+ I  + GV+A+K++ EM S+E G
Sbjct: 163 GAVKEEE----------VRVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENG 222

Query: 203 IVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVCQQAPICCHIWFVC 262
           IV++I++  I+ LVMG  A+        H+  RR T LKS+KA +V ++AP  C IWF C
Sbjct: 223 IVQLISELGIRKLVMGAAAD-------RHY-SRRMTDLKSRKAIFVRREAPTLCQIWFTC 282

Query: 263 SGRLIYSREGRMGRFNPL------------LLQNSEIVIDQSNHLRPESVTFKLNFL--- 322
            G LI++RE  M                  LLQ       +  H+     T  +  L   
Sbjct: 283 KGYLIHTREATMDDTESEYASPRPSISASDLLQTFSTPESEHQHISRVQSTDSVQQLVSN 342

Query: 323 -----EDSRITLLQNFADAQEKEDAFDRI---SRFRSEGLVDPKCSNNGVMSTSRTRLLL 382
                +  R++      D +E+E     +   +   S G   P    +GV  +       
Sbjct: 343 GSSTEQSGRVSDGSLNTDEEERESDGSEVTGSATVMSSGHSSPSSFPDGVDDSF------ 402

Query: 383 KNEGVKGGQPVSQSRGLQEEAVLNVKSIKEFEGVRVKEDAMGANFENKSLVSSCMEEVKK 442
               VK  +  S++   ++EA       +     + +++A+ A    K   S+  EE+K+
Sbjct: 403 ---NVKIRKATSEAHSSKQEAF-----AETLRRQKAEKNALDAIRRAKQSESAYSEELKR 462

Query: 443 RKEMEEILVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEA 502
           RK+ E  + +EK+    +  E + +++EL     QK++LE + ++    +E L +K+  A
Sbjct: 463 RKDTEIAVAKEKERFITIKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIA 522

Query: 503 VDLLISFKEKRDKLQIEHEGAMNKLRQLKNMVKREPTCSVEMP----TFSFMEIIEATRN 562
           V LL   +++R++LQ E + A+ +  +L++  +   T ++++P     FSF EI EAT +
Sbjct: 523 VKLLQKLRDEREELQTERDRALREAEELRSHAE---TSTLQLPQYFTDFSFSEIEEATNH 582

Query: 563 FDPSWKIGEGRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSII 622
           FD + KIGEG +GS+Y G LRH  VA+KML    S     +Q EV+VLS++RHPN++++I
Sbjct: 583 FDSTLKIGEGGYGSIYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLI 642

Query: 623 GACPESRLIVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEP-CIV 682
           GACPE   +VYE+L   SLE  L CKD++ PL WQ R+R+A +IC+AL+FLH ++   +V
Sbjct: 643 GACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLV 702

Query: 683 HGDIKPSKILLDANFVAKLGGLGISRLI-PQEEKAFSSDSAYNMSKENNPYIDPEYLETG 742
           HGD+KP+ ILLD+N V+KL   G   L+ P   K+  +D    ++     Y+DPE   +G
Sbjct: 703 HGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVA-----YLDPEASSSG 762

Query: 743 RFTPESDVYSLGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLA 802
             TP+SDVYS G+  LR+LTGR    I  +VK AL+N  ++ +LD  AGDWP   AEQLA
Sbjct: 763 ELTPKSDVYSFGIILLRLLTGRPALRISNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLA 822

Query: 803 LVALRCCEKEKSDRPDLVSELWSVLEPMRSIASASFS---SSKKHSRVPAHFTCPIFQEI 862
            +ALRCCE    +RPDL +E+W VLEPMR+ +  S S      +H   P +F CPIFQE+
Sbjct: 823 RLALRCCETVSENRPDLGTEVWRVLEPMRASSGGSSSFHLGRNEHRIAPPYFICPIFQEV 834

Query: 863 MKDPLIAADGFTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEW-QHQL 889
           M+DP +AADGFTYEA+AIR W  S HDTSPMTN+KL H +L+ N+AL +AIQEW QH L
Sbjct: 883 MQDPHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEWLQHHL 834

BLAST of Spg002406 vs. ExPASy Swiss-Prot
Match: Q5WA76 (U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB70 PE=1 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.0e-109
Identity = 237/527 (44.97%), Postives = 324/527 (61.48%), Query Frame = 0

Query: 374 RGLQEEAVLNVKSIKEFEGVRVKEDAMGANFENKSLVSSCMEEVKKRKEMEEILVREKKE 433
           + + E AVL  K   +    R++ + +    E+  L      +V++RKE EE L R ++E
Sbjct: 301 KSMDEAAVLK-KEASDERLKRIESERLARTLEDLYL-----SQVQQRKETEESLSRVQQE 360

Query: 434 LERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISFKEKRDKL 493
            E++  + DE+  EL  V EQ   L  + S+ R           E  + L+S   + D+L
Sbjct: 361 FEQLKIQQDEVTVELQRVNEQNENLLGQLSDSR-----------EHFEWLLS---EHDQL 420

Query: 494 QIEHEGAMNKLRQLKNMVKREPTCSVEMPT----FSFMEIIEATRNFDPSWKIGEGRHGS 553
             E + A+ ++ +L+   KR    SV +      FS  E+  AT NF  S KIGEG  G 
Sbjct: 421 LRERDNAVREVEELRQ--KRGQMLSVLVTAMHCEFSSSEVESATENFSNSLKIGEGGFGC 480

Query: 554 VYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVYEHL 613
           VYKG LR+M VA+K+L       QS+F+ EV +LSRVRHP+LV+++GAC ES  +VYE L
Sbjct: 481 VYKGILRNMTVAIKVLRPDSLQGQSQFEQEVSILSRVRHPHLVTLLGACSESSTLVYEFL 540

Query: 614 TNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEP-CIVHGDIKPSKILLDAN 673
            N SLE  L C D  + L WQ RIR+ A+ICSALIFLH ++P  +VHGD+KP+ ILL  N
Sbjct: 541 PNGSLEDFLMCSDKRQTLTWQARIRIIAEICSALIFLHKNKPHPVVHGDLKPANILLGVN 600

Query: 674 FVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNP-----YIDPEYLETGRFTPESDVYS 733
            V+KL   GISRL+ Q     SS +   + +  +P     Y+DPE+L TG  TP+SDVYS
Sbjct: 601 LVSKLSDFGISRLLIQ-----SSTNNTTLYRTMHPVGTPLYMDPEFLSTGELTPQSDVYS 660

Query: 734 LGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKE 793
            G+  LR+LTG+ P GI   V+ A+E   +++V+D+S G+WPH   EQLA +ALRC E  
Sbjct: 661 FGIVVLRLLTGKPPVGIKNIVEDAMEKGDLNSVIDTSVGEWPHLHIEQLAYLALRCTELS 720

Query: 794 KSDRPDLVSELWSVLEPMRSIASASFSSSKK---HSRVPAHFTCPIFQEIMKDPLIAADG 853
           +  RPDL  E+W+++E +R  A +S SSS+     +  P++F CPI Q+IM DP IAADG
Sbjct: 721 RRCRPDLSGEVWAIVEAIRDAALSSPSSSRSAQDQNSPPSYFICPISQDIMDDPHIAADG 780

Query: 854 FTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQ 888
           FTYEA+AIR W  +GHDTSPMTNL LEH  L+PN AL +AIQEW  Q
Sbjct: 781 FTYEAEAIRSWLCNGHDTSPMTNLLLEHEELIPNRALRSAIQEWLQQ 800

BLAST of Spg002406 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 3.4e-84
Identity = 186/517 (35.98%), Postives = 302/517 (58.41%), Query Frame = 0

Query: 388 KEFEGVRVKEDAMGANFENKSLVSSCMEEVKKRKEMEEILVREKKELERMNKEHDELLKE 447
           ++F+  +    +M +NFE  ++V+         ++    + + + EL   ++ +   + +
Sbjct: 310 RDFDERKDAMSSMSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMY--AVAQ 369

Query: 448 LHDVQEQKSLLERKASEYRC---DVEDLEKKMFEAVDLLISFKEKRDKLQIEHEGAMNKL 507
           +  +   + L E K  E      + + + KK  E  +     +E+R+  Q   E  M   
Sbjct: 370 VETLDASRKLNELKFEELTLLEHETKGIAKKETEKFEQ--KRREEREAAQ-RREAEMKAT 429

Query: 508 RQLKNMVKREPTCSV----EMPTFSFMEIIEATRNFDPSWKIGEGRHGSVYKGFLRHMDV 567
            + K   K E +  V    +   F++ EII AT +F    KIG G +G VYK  L H   
Sbjct: 430 HEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIA 489

Query: 568 ALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVYEHLTNSSLESHLAC 627
           A+K+L S  S    +F  E+E+LS++RHP+LV ++GACP+   +VYE++ N SLE  L  
Sbjct: 490 AVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQ 549

Query: 628 KDHNRPLPWQIRIRVAADICSALIFLHYSEPC-IVHGDIKPSKILLDANFVAKLGGLGIS 687
            + ++P+PW +R+R+A ++ SAL+FLH S+P  I+H D+KP+ ILL+ NFV+K+G +G+S
Sbjct: 550 VNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLS 609

Query: 688 RLIPQEEKAFSSDSAYNMSKENNP-----YIDPEYLETGRFTPESDVYSLGVTFLRILTG 747
            +I   + A    + + M K+ +P     YIDPEY  TGR +P+SDVY+ G+  L++LTG
Sbjct: 610 TMI---QAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTG 669

Query: 748 RAPSGIVKDVKCALEN---DKISTVLDSSAGDWPHDLAEQLALVALRCCEKEKSDRPDLV 807
           +    +   V+ A+EN   D++  +LD  AG+WP +   QLA +AL+C E    DRPDL 
Sbjct: 670 QQAMALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLE 729

Query: 808 SELWSVLEPMRSIA-SASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYEADAIRG 867
            ++  VLE ++ +A  A  S S   S+ P+HF CP+ +++MK+P IAADG+TY+  AI  
Sbjct: 730 DQILPVLESLKKVADKARNSLSAAPSQPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEE 789

Query: 868 WFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQ 888
           W ++ H TSP+TN  L++ NL+PN+ L  AI EW+++
Sbjct: 790 WMEN-HRTSPVTNSPLQNVNLLPNHTLYAAIVEWRNR 817

BLAST of Spg002406 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 302.4 bits (773), Expect = 1.7e-80
Identity = 226/768 (29.43%), Postives = 367/768 (47.79%), Query Frame = 0

Query: 152 SNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQAQKVWTEMNSVERGIVEIIAQYS 211
           S T K+     +++   +K  E+L     +     VQ   +  E + +   I + +  + 
Sbjct: 52  STTRKDLTTSVYKKDVDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHG 111

Query: 212 IKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVCQQAPICCHIWFVCSGRLIYSRE 271
           I  LV+G    S + +     +       +S  +  +    P  C +  +  G+L+  R+
Sbjct: 112 ISELVIG---ASSSIIFSWKLK-------RSNLSSRIADATPRFCSVHVISKGKLLNVRK 171

Query: 272 GRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNF----LEDSRITLLQNF-------AD 331
             M     +    SE      +H    S T    F    L   RI  L            
Sbjct: 172 SDMDTETSIADDRSESRFSSDSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIG 231

Query: 332 AQEKEDAFDRISRFRSEGLVDPKCSN-NGVMSTSRTRLLLKNEGVKGGQPVSQSRGLQEE 391
            Q  E      +R  S  + + K  N  G   TS + +        GG  +S  R  Q E
Sbjct: 232 KQNNEPHHHHHNRAGSLDVDESKLLNQKGFYRTSSSGI------GYGGSDISSWRSSQME 291

Query: 392 AVLNVKSIKEFEGVRVKEDAMGANFENKSLVSSCMEEVKKRKEMEEILVREKKELERMNK 451
                 S   +         +  +FE + L              +  ++   K+++ +N+
Sbjct: 292 ---EASSSSTYSDPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQ 351

Query: 452 EHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISFKEKRDKLQIEHEG 511
              E    L ++  ++   +      R   ED E +     + +    E+R + +   E 
Sbjct: 352 RRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEE 411

Query: 512 AMNKLRQLKNMVKREPTCSVEMPTFSFMEIIEATRNFDPSWKIGEGRHGSVYKGFLRHMD 571
              + ++L++ ++  P    +   F + EI+EAT +F    KIG G +GSVY+  L H  
Sbjct: 412 VRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTT 471

Query: 572 VALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVYEHLTNSSLESHLA 631
           VA+K+L S  S    +F  E+E+LS++RHP+L+ ++GACPE   +VYE++ N SLE  L 
Sbjct: 472 VAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLM 531

Query: 632 CKDHN------RPLPWQIRIRVAADICSALIFLHYSEP-CIVHGDIKPSKILLDANFVAK 691
            +  N       PL W  R R+A +I SAL FLH +EP  IVH D+KP+ ILLD N V+K
Sbjct: 532 KRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSK 591

Query: 692 LGGLGISRLIPQEEKAFSSDSAYNMSKENNP-----YIDPEYLETGRFTPESDVYSLGVT 751
           +G +G+S+++           A  +  E  P     YIDPEY  TG  TPESD+Y+ G+ 
Sbjct: 592 IGDVGLSKMV-----NLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGII 651

Query: 752 FLRILTGRAPSGIVKDVKCAL--ENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKEKS 811
            L+++T R+  G+   ++ AL  +  K + +LD +AGDWP   A+++ ++ LRC E  K 
Sbjct: 652 LLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKR 711

Query: 812 DRPDLVSELWSVLEPMRSIASAS---FSSS--KKHSRVPAHFTCPIFQEIMKDPLIAADG 871
           DRPDL  E+  VLE ++ +AS +   F+ +    H   P HF CPI +++M++P +A+DG
Sbjct: 712 DRPDLGKEILPVLERLKEVASIARNMFADNLIDHHHNAPTHFYCPITKDVMENPCVASDG 771

Query: 872 FTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           +TYE  AI+ W +  H  SPMT+L     +L+PN++LL+AI+EW+ QL
Sbjct: 772 YTYEKRAIKEWLQKNH-KSPMTDLPFPSDSLLPNHSLLSAIKEWRSQL 794

BLAST of Spg002406 vs. ExPASy TrEMBL
Match: A0A6J1CGH6 (U-box domain-containing protein 32 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011460 PE=4 SV=1)

HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 650/885 (73.45%), Postives = 704/885 (79.55%), Query Frame = 0

Query: 10  GTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVAQ 69
           GTAEEIGQ+L FDVE TIFVAVG +VD+TK+TL+WAV NF GKNFCLLHVH P       
Sbjct: 3   GTAEEIGQQLVFDVEHTIFVAVGTNVDETKTTLIWAVHNFAGKNFCLLHVHPPP------ 62

Query: 70  SEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKAL 129
                                                                       
Sbjct: 63  ------------------------------------------------------------ 122

Query: 130 ISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQA 189
                       G  + NE PSSNTLKE +AKAFEEP RQKP E LNQYV ILAKLGVQA
Sbjct: 123 -----------PGVGLRNEGPSSNTLKEQIAKAFEEPGRQKPFEPLNQYVAILAKLGVQA 182

Query: 190 QKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVC 249
           QKVW E NSVERGIVEIIAQYSIKWLVMGLDAE  N         +R TGLKS KAF+VC
Sbjct: 183 QKVWIETNSVERGIVEIIAQYSIKWLVMGLDAERYNL--------KRSTGLKSNKAFFVC 242

Query: 250 QQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLED 309
           +QAP+CCHIW VC G LIYSREGRMGRF+P LLQN +I  +QSN+L+PESVT +L+FL  
Sbjct: 243 EQAPMCCHIWLVCRGHLIYSREGRMGRFHPPLLQNLDIGTNQSNNLKPESVTCQLSFL-- 302

Query: 310 SRITLLQNFADAQEKEDAFDRI-SRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVK-GG 369
                     D QEKE+AFD + SRFRS+GLVDPKCSNNG++ TSRT LLLKNEGVK  G
Sbjct: 303 ----------DTQEKENAFDGVLSRFRSQGLVDPKCSNNGILGTSRTILLLKNEGVKERG 362

Query: 370 QPVSQSRGLQEEAVLNVKSIKEFEGVRV--KEDAMGANFENKSLVSSCMEEVKKRKEMEE 429
           +PV Q  GLQ EA++NVK IKEF GV+   +EDA GA F+ KS+ SSCMEEVK+ KEMEE
Sbjct: 363 RPVIQGPGLQ-EAMINVKPIKEFAGVKAWEEEDAAGAKFKAKSIESSCMEEVKEGKEMEE 422

Query: 430 ILVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLIS 489
           +L REK E+E+MNKE DELLKELHDVQEQ  LL RKASEY+CDV+ LE+K+F AVDLL+S
Sbjct: 423 LLEREKLEVEKMNKERDELLKELHDVQEQTLLLGRKASEYQCDVKKLEEKLFAAVDLLVS 482

Query: 490 FKEKRDKLQIEHEGAMNKLRQLKNMVKREPTC--SVEMPTFSFMEIIEATRNFDPSWKIG 549
           FKEKRDKLQIEHEGAMNKLRQLKNMVK EP    S EMPTFSFMEIIEATRNFDPSWKIG
Sbjct: 483 FKEKRDKLQIEHEGAMNKLRQLKNMVKGEPPYFRSAEMPTFSFMEIIEATRNFDPSWKIG 542

Query: 550 EGRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRL 609
           EGRHGSVYKG LRHMDVA+KM PSYGSHSQS+FQYEVEVLSRVRHPNLVSIIGACPESRL
Sbjct: 543 EGRHGSVYKGLLRHMDVAIKMFPSYGSHSQSKFQYEVEVLSRVRHPNLVSIIGACPESRL 602

Query: 610 IVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKI 669
           IVYE+L NSSLE  LACKDHNRPLPWQIRIRVAADICSALIFLHYS+PCIVHGDIKPSKI
Sbjct: 603 IVYENLKNSSLEDQLACKDHNRPLPWQIRIRVAADICSALIFLHYSKPCIVHGDIKPSKI 662

Query: 670 LLDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYS 729
           LLDANF+AKLGGLGISRLIP+EE AF+S  AY+MSKENNPYIDPEYL+TGRFTPESD+YS
Sbjct: 663 LLDANFIAKLGGLGISRLIPREENAFNSAFAYDMSKENNPYIDPEYLQTGRFTPESDIYS 722

Query: 730 LGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKE 789
            GVT LRILTGRAPSGIVKDVKCALEN+KI  +LDSSAGDWPHDLAEQLALVALRCCE++
Sbjct: 723 FGVTLLRILTGRAPSGIVKDVKCALENNKIGAILDSSAGDWPHDLAEQLALVALRCCERD 782

Query: 790 KSDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTY 849
           KS+R DLVSELWSVLEPMRSIAS SFSS KKHSRVPA FTCPIFQEIMKDPLIAADGFTY
Sbjct: 783 KSNRLDLVSELWSVLEPMRSIASTSFSSMKKHSRVPAQFTCPIFQEIMKDPLIAADGFTY 789

Query: 850 EADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           EADAIRGWF SGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 843 EADAIRGWFGSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 789

BLAST of Spg002406 vs. ExPASy TrEMBL
Match: A0A1S3AZ09 (U-box domain-containing protein 32 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484094 PE=4 SV=1)

HSP 1 Score: 1192.9 bits (3085), Expect = 0.0e+00
Identity = 636/884 (71.95%), Postives = 693/884 (78.39%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MG+A+EIG  LQFDVE+TIFVAVG DVDD K+TL+WAVQNF GK FCLLHV+        
Sbjct: 1   MGSADEIGLPLQFDVEDTIFVAVGTDVDDAKATLVWAVQNFAGKIFCLLHVYH------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     L P+              
Sbjct: 61  ------------------------------------------LPPK-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                             NEEPSS TLKEHV +AFEEPERQKP ELLNQYVLILAKLGVQ
Sbjct: 121 ------------------NEEPSSYTLKEHVGQAFEEPERQKPFELLNQYVLILAKLGVQ 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYV 248
           AQKVW E N+VERGIVEIIAQYSIKWLVMGLD E         +  +R  GLKSKKAFYV
Sbjct: 181 AQKVWIETNNVERGIVEIIAQYSIKWLVMGLDTE--------RYNMKRSIGLKSKKAFYV 240

Query: 249 CQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLE 308
            QQAPI CHIWFVC GRLIYSREGRMGR      ++SE+ I+ ++HL+PESVT K     
Sbjct: 241 SQQAPIWCHIWFVCRGRLIYSREGRMGR-----SKSSEVGINYTDHLKPESVTCK----- 300

Query: 309 DSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQ 368
                   NFADAQEKE+A DRISRFR +GLVD K  NNG + TSRT LLLKNEGVK G 
Sbjct: 301 --------NFADAQEKENAGDRISRFRYQGLVDQKLYNNGDLGTSRTTLLLKNEGVKEGH 360

Query: 369 PVSQSRGLQEEAVLNVKSIKEFEGVR--VKEDAMGANFENKSLVSSCMEEVKKRKEMEEI 428
           PVSQS GLQ EA +N KSIK+FEGV+  V++D +GA F+ +SL SSCMEEVKKRKEMEE+
Sbjct: 361 PVSQS-GLQ-EASMNGKSIKDFEGVKAWVEKDTVGAEFKAESLESSCMEEVKKRKEMEEL 420

Query: 429 LVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISF 488
           L +EKKE+ERMNKE D LL+EL  V+EQKS+LERKASEY+ D+E+LEKKMF AVDLL+SF
Sbjct: 421 LEKEKKEVERMNKERDGLLEELQHVEEQKSVLERKASEYQSDMEELEKKMFAAVDLLVSF 480

Query: 489 KEKRDKLQIEHEGAMNKLRQLKNMVKREPT--CSVEMPTFSFMEIIEATRNFDPSWKIGE 548
           KEKRDKL IE EGAM+KLR+LKN+VKREP+   + EMP FSF+EIIEATRNFDPSWKIGE
Sbjct: 481 KEKRDKLLIEREGAMDKLRKLKNIVKREPSRYHNAEMPMFSFVEIIEATRNFDPSWKIGE 540

Query: 549 GRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLI 608
           GRHGSVYKG LRHMDVALKM PSYGSHSQS FQYEVEVLSRVRHPNLVSIIGACPESRLI
Sbjct: 541 GRHGSVYKGLLRHMDVALKMFPSYGSHSQSAFQYEVEVLSRVRHPNLVSIIGACPESRLI 600

Query: 609 VYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKIL 668
           VYE+L N SLE HLACK+HN PLPWQ RIR+AADICSALIFLHYS+PCIVHGDIKPSKIL
Sbjct: 601 VYENLKNGSLEDHLACKNHNCPLPWQTRIRIAADICSALIFLHYSDPCIVHGDIKPSKIL 660

Query: 669 LDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYSL 728
           LD NF+AKLGGLGISRLIPQEEKAF+S S  N+SKENNPYIDPEYLETG+FT ESDVYS+
Sbjct: 661 LDTNFIAKLGGLGISRLIPQEEKAFNSASVCNISKENNPYIDPEYLETGKFTSESDVYSV 720

Query: 729 GVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKEK 788
           GV  LRILTGR P GIVKDVKCA+END I  VLDSSAGDWPHDLAEQLALVALRCCEKEK
Sbjct: 721 GVILLRILTGRTPPGIVKDVKCAIENDNIVVVLDSSAGDWPHDLAEQLALVALRCCEKEK 775

Query: 789 SDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYE 848
            DRPDLVSELW VLEPMRSIASAS SS KKHSRVPAHFTCPIFQEIMKDPLIAADGFTYE
Sbjct: 781 LDRPDLVSELWCVLEPMRSIASASCSSLKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYE 775

Query: 849 ADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           ADAIRGWF+SGH+TSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 ADAIRGWFQSGHNTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 775

BLAST of Spg002406 vs. ExPASy TrEMBL
Match: A0A1S3AYB6 (U-box domain-containing protein 32 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484094 PE=4 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 636/885 (71.86%), Postives = 693/885 (78.31%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MG+A+EIG  LQFDVE+TIFVAVG DVDD K+TL+WAVQNF GK FCLLHV+        
Sbjct: 1   MGSADEIGLPLQFDVEDTIFVAVGTDVDDAKATLVWAVQNFAGKIFCLLHVYH------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     L P+              
Sbjct: 61  ------------------------------------------LPPK-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                             NEEPSS TLKEHV +AFEEPERQKP ELLNQYVLILAKLGVQ
Sbjct: 121 ------------------NEEPSSYTLKEHVGQAFEEPERQKPFELLNQYVLILAKLGVQ 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYV 248
           AQKVW E N+VERGIVEIIAQYSIKWLVMGLD E         +  +R  GLKSKKAFYV
Sbjct: 181 AQKVWIETNNVERGIVEIIAQYSIKWLVMGLDTE--------RYNMKRSIGLKSKKAFYV 240

Query: 249 CQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLE 308
            QQAPI CHIWFVC GRLIYSREGRMGR      ++SE+ I+ ++HL+PESVT K     
Sbjct: 241 SQQAPIWCHIWFVCRGRLIYSREGRMGR-----SKSSEVGINYTDHLKPESVTCK----- 300

Query: 309 DSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQ 368
                   NFADAQEKE+A DRISRFR +GLVD K  NNG + TSRT LLLKNEGVK G 
Sbjct: 301 --------NFADAQEKENAGDRISRFRYQGLVDQKLYNNGDLGTSRTTLLLKNEGVKEGH 360

Query: 369 PVSQSRGLQEEAVLNVKSIKEFEGVR--VKEDAMGANFENKSLVSSCMEEVKKRKEMEEI 428
           PVSQS GLQ EA +N KSIK+FEGV+  V++D +GA F+ +SL SSCMEEVKKRKEMEE+
Sbjct: 361 PVSQS-GLQ-EASMNGKSIKDFEGVKAWVEKDTVGAEFKAESLESSCMEEVKKRKEMEEL 420

Query: 429 LVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISF 488
           L +EKKE+ERMNKE D LL+EL  V+EQKS+LERKASEY+ D+E+LEKKMF AVDLL+SF
Sbjct: 421 LEKEKKEVERMNKERDGLLEELQHVEEQKSVLERKASEYQSDMEELEKKMFAAVDLLVSF 480

Query: 489 KEKRDKLQIEHEGAMNKLRQLKNMVKREPT--CSVEMPTFSFMEIIEATRNFDPSWKIGE 548
           KEKRDKL IE EGAM+KLR+LKN+VKREP+   + EMP FSF+EIIEATRNFDPSWKIGE
Sbjct: 481 KEKRDKLLIEREGAMDKLRKLKNIVKREPSRYHNAEMPMFSFVEIIEATRNFDPSWKIGE 540

Query: 549 GRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYE-VEVLSRVRHPNLVSIIGACPESRL 608
           GRHGSVYKG LRHMDVALKM PSYGSHSQS FQYE VEVLSRVRHPNLVSIIGACPESRL
Sbjct: 541 GRHGSVYKGLLRHMDVALKMFPSYGSHSQSAFQYEQVEVLSRVRHPNLVSIIGACPESRL 600

Query: 609 IVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKI 668
           IVYE+L N SLE HLACK+HN PLPWQ RIR+AADICSALIFLHYS+PCIVHGDIKPSKI
Sbjct: 601 IVYENLKNGSLEDHLACKNHNCPLPWQTRIRIAADICSALIFLHYSDPCIVHGDIKPSKI 660

Query: 669 LLDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYS 728
           LLD NF+AKLGGLGISRLIPQEEKAF+S S  N+SKENNPYIDPEYLETG+FT ESDVYS
Sbjct: 661 LLDTNFIAKLGGLGISRLIPQEEKAFNSASVCNISKENNPYIDPEYLETGKFTSESDVYS 720

Query: 729 LGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKE 788
           +GV  LRILTGR P GIVKDVKCA+END I  VLDSSAGDWPHDLAEQLALVALRCCEKE
Sbjct: 721 VGVILLRILTGRTPPGIVKDVKCAIENDNIVVVLDSSAGDWPHDLAEQLALVALRCCEKE 776

Query: 789 KSDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTY 848
           K DRPDLVSELW VLEPMRSIASAS SS KKHSRVPAHFTCPIFQEIMKDPLIAADGFTY
Sbjct: 781 KLDRPDLVSELWCVLEPMRSIASASCSSLKKHSRVPAHFTCPIFQEIMKDPLIAADGFTY 776

Query: 849 EADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           EADAIRGWF+SGH+TSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 EADAIRGWFQSGHNTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 776

BLAST of Spg002406 vs. ExPASy TrEMBL
Match: A0A1S3AXZ2 (U-box domain-containing protein 32 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484094 PE=4 SV=1)

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 631/885 (71.30%), Postives = 689/885 (77.85%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MG+A+EIG  LQFDVE+TIFVAVG DVDD K+TL+WAVQNF GK FCLLHV+        
Sbjct: 1   MGSADEIGLPLQFDVEDTIFVAVGTDVDDAKATLVWAVQNFAGKIFCLLHVYH------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     L P+              
Sbjct: 61  ------------------------------------------LPPK-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                             NEEPSS TLKEHV +AFEEPERQKP ELLNQYVLILAKLGVQ
Sbjct: 121 ------------------NEEPSSYTLKEHVGQAFEEPERQKPFELLNQYVLILAKLGVQ 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYV 248
           AQKVW E N+VERGIVEIIAQYSIKWLVMGLD E         +  +R  GLKSKKAFYV
Sbjct: 181 AQKVWIETNNVERGIVEIIAQYSIKWLVMGLDTE--------RYNMKRSIGLKSKKAFYV 240

Query: 249 CQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLE 308
            QQAPI CHIWFVC GRLIYSREGRMGR      ++SE+ I+ ++HL+PESVT+      
Sbjct: 241 SQQAPIWCHIWFVCRGRLIYSREGRMGR-----SKSSEVGINYTDHLKPESVTY------ 300

Query: 309 DSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQ 368
                       AQEKE+A DRISRFR +GLVD K  NNG + TSRT LLLKNEGVK G 
Sbjct: 301 ------------AQEKENAGDRISRFRYQGLVDQKLYNNGDLGTSRTTLLLKNEGVKEGH 360

Query: 369 PVSQSRGLQEEAVLNVKSIKEFEGVR--VKEDAMGANFENKSLVSSCMEEVKKRKEMEEI 428
           PVSQS GLQ EA +N KSIK+FEGV+  V++D +GA F+ +SL SSCMEEVKKRKEMEE+
Sbjct: 361 PVSQS-GLQ-EASMNGKSIKDFEGVKAWVEKDTVGAEFKAESLESSCMEEVKKRKEMEEL 420

Query: 429 LVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISF 488
           L +EKKE+ERMNKE D LL+EL  V+EQKS+LERKASEY+ D+E+LEKKMF AVDLL+SF
Sbjct: 421 LEKEKKEVERMNKERDGLLEELQHVEEQKSVLERKASEYQSDMEELEKKMFAAVDLLVSF 480

Query: 489 KEKRDKLQIEHEGAMNKLRQLKNMVKREPT--CSVEMPTFSFMEIIEATRNFDPSWKIGE 548
           KEKRDKL IE EGAM+KLR+LKN+VKREP+   + EMP FSF+EIIEATRNFDPSWKIGE
Sbjct: 481 KEKRDKLLIEREGAMDKLRKLKNIVKREPSRYHNAEMPMFSFVEIIEATRNFDPSWKIGE 540

Query: 549 GRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYE-VEVLSRVRHPNLVSIIGACPESRL 608
           GRHGSVYKG LRHMDVALKM PSYGSHSQS FQYE VEVLSRVRHPNLVSIIGACPESRL
Sbjct: 541 GRHGSVYKGLLRHMDVALKMFPSYGSHSQSAFQYEQVEVLSRVRHPNLVSIIGACPESRL 600

Query: 609 IVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKI 668
           IVYE+L N SLE HLACK+HN PLPWQ RIR+AADICSALIFLHYS+PCIVHGDIKPSKI
Sbjct: 601 IVYENLKNGSLEDHLACKNHNCPLPWQTRIRIAADICSALIFLHYSDPCIVHGDIKPSKI 660

Query: 669 LLDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYS 728
           LLD NF+AKLGGLGISRLIPQEEKAF+S S  N+SKENNPYIDPEYLETG+FT ESDVYS
Sbjct: 661 LLDTNFIAKLGGLGISRLIPQEEKAFNSASVCNISKENNPYIDPEYLETGKFTSESDVYS 720

Query: 729 LGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKE 788
           +GV  LRILTGR P GIVKDVKCA+END I  VLDSSAGDWPHDLAEQLALVALRCCEKE
Sbjct: 721 VGVILLRILTGRTPPGIVKDVKCAIENDNIVVVLDSSAGDWPHDLAEQLALVALRCCEKE 771

Query: 789 KSDRPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTY 848
           K DRPDLVSELW VLEPMRSIASAS SS KKHSRVPAHFTCPIFQEIMKDPLIAADGFTY
Sbjct: 781 KLDRPDLVSELWCVLEPMRSIASASCSSLKKHSRVPAHFTCPIFQEIMKDPLIAADGFTY 771

Query: 849 EADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           EADAIRGWF+SGH+TSPMTNLKLEHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 EADAIRGWFQSGHNTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 771

BLAST of Spg002406 vs. ExPASy TrEMBL
Match: A0A6J1EAK1 (U-box domain-containing protein 32-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430841 PE=4 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 630/882 (71.43%), Postives = 679/882 (76.98%), Query Frame = 0

Query: 9   MGTAEEIGQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVA 68
           MGTAEE  Q LQFDVE+TIFVAVG D DD K+TLLWAVQNF GK+FCLLHVHQ       
Sbjct: 1   MGTAEETVQHLQFDVEDTIFVAVGTDADDAKATLLWAVQNFAGKSFCLLHVHQ------- 60

Query: 69  QSEFCSYGISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKA 128
                                                     L PR              
Sbjct: 61  ------------------------------------------LTPR-------------- 120

Query: 129 LISYLPLNQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQ 188
                          + +NE+PSSNTLKEHVAKAFEE ERQK  ELLNQYVLILAKLGV+
Sbjct: 121 -------------ASSPKNEDPSSNTLKEHVAKAFEELERQKSFELLNQYVLILAKLGVR 180

Query: 189 AQKVWTEMNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYV 248
           A+K+  E NSVERGI+EIIAQYS+KWLVMGLDAE  N         +  TGLKSKKAF+V
Sbjct: 181 AEKILIETNSVERGILEIIAQYSVKWLVMGLDAERYNL--------KMSTGLKSKKAFFV 240

Query: 249 CQQAPICCHIWFVCSGRLIYSREGRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNFLE 308
            QQAP+CCHIWFVC GRL+YSREG  GRFN L L N+EI I+ +NHLRPESVT KLN L 
Sbjct: 241 SQQAPLCCHIWFVCRGRLVYSREGGPGRFNKLQLPNTEIGINHTNHLRPESVTCKLNLL- 300

Query: 309 DSRITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQ 368
                      DAQE+E+AFD ISRF  EG VDP   N G   TSRT LLLKNE      
Sbjct: 301 -----------DAQEQENAFDSISRFIYEGPVDPMFENLG---TSRTTLLLKNE------ 360

Query: 369 PVSQSRGLQEEAVLNVKSIKEFEGVRVKEDAMGANFENKSLVSSCMEEVKKRKEMEEILV 428
                 GLQE A +NV  IKEFE     E+A+GA F+ KSL SSC+EEVK+RKEMEEIL 
Sbjct: 361 ----VNGLQEAASMNVTPIKEFE-----EEAVGAEFKAKSLESSCVEEVKRRKEMEEILA 420

Query: 429 REKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISFKE 488
             KKE+ERMNKEHD+LLKELH V EQKSLLERKASE RCDVE+LEKKMF AVDLL+SFKE
Sbjct: 421 SSKKEVERMNKEHDKLLKELHRVHEQKSLLERKASESRCDVEELEKKMFAAVDLLVSFKE 480

Query: 489 KRDKLQIEHEGAMNKLRQLKNMVKREPTCS--VEMPTFSFMEIIEATRNFDPSWKIGEGR 548
           KRDKLQIE E A N+LR+LK MV+REP+C+   EMPTFSFMEIIEATRNFDPSWKIGEGR
Sbjct: 481 KRDKLQIEQEDATNELRKLKKMVEREPSCNRRAEMPTFSFMEIIEATRNFDPSWKIGEGR 540

Query: 549 HGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVY 608
           HGSVYKG LRHMDVALKM PSYGSHSQS FQYEVE+LSRVRHPNLVSIIGACPESRLIVY
Sbjct: 541 HGSVYKGVLRHMDVALKMFPSYGSHSQSTFQYEVELLSRVRHPNLVSIIGACPESRLIVY 600

Query: 609 EHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEPCIVHGDIKPSKILLD 668
           E++ NSSLE HLACK  NRPLPWQIRIRVAADIC ALIFLHYSEPCIVHGDIKPSKILLD
Sbjct: 601 ENMKNSSLEDHLACK--NRPLPWQIRIRVAADICLALIFLHYSEPCIVHGDIKPSKILLD 660

Query: 669 ANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENNPYIDPEYLETGRFTPESDVYSLGV 728
           ANF+AKL GLGISRLIPQE+KAF S S Y++SKENN YIDPEYLETGR TPESDVYSLGV
Sbjct: 661 ANFIAKLSGLGISRLIPQEQKAFDSASVYSLSKENNSYIDPEYLETGRLTPESDVYSLGV 720

Query: 729 TFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKEKSD 788
           T LRILTGRAP  IV DVKCALEND+I  +LDSSAGDWPHDLAEQLALVALRCCEKEKS+
Sbjct: 721 TLLRILTGRAPPSIVNDVKCALENDQIGAILDSSAGDWPHDLAEQLALVALRCCEKEKSN 766

Query: 789 RPDLVSELWSVLEPMRSIASASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYEAD 848
           RPDLVSELWSVLEPMRSIASAS  + KKHSRVPAHF CPIFQEIMKDPLIAADGFTYEAD
Sbjct: 781 RPDLVSELWSVLEPMRSIASASCPNLKKHSRVPAHFACPIFQEIMKDPLIAADGFTYEAD 766

Query: 849 AIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           AIRGWF+SGH+TSPMTNLK+EHCNLVPNYALLNAIQEWQHQL
Sbjct: 841 AIRGWFESGHNTSPMTNLKVEHCNLVPNYALLNAIQEWQHQL 766

BLAST of Spg002406 vs. TAIR 10
Match: AT3G49060.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 579.3 bits (1492), Expect = 5.2e-165
Identity = 356/919 (38.74%), Postives = 518/919 (56.37%), Query Frame = 0

Query: 16  GQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVAQSEFCSY 75
           G++L  DV+ETIFVAV  DV+ +K+T+LWA +NF GK  CLL+VH+ A            
Sbjct: 6   GEELVLDVDETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTAR----------- 65

Query: 76  GISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKALISYLPL 135
                            + W                                        
Sbjct: 66  ----------------AASW---------------------------------------- 125

Query: 136 NQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQAQKVWTE 195
              + VGG          + K+H  K  E  E+ K  EL+N Y+ +L++  +Q  K+   
Sbjct: 126 THKKLVGG----------SFKKHDVKVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIA 185

Query: 196 MNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVCQQAPIC 255
             ++E  IVE+IA++ IKWLVMG  A SD      H+   + T LKSKKA +VC++AP  
Sbjct: 186 GQNIEECIVELIARHKIKWLVMG--AASDK-----HY-SWKMTDLKSKKAIFVCKKAPDS 245

Query: 256 CHIWFVCSGRLIYSREGRMGRFN-----PLLLQNSEIVIDQSNHLRPESVTFKLNFLEDS 315
           CHIWF+C G LI++R       N     PL+  +S+    +S  L    +  +L +    
Sbjct: 246 CHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQLDSDNETRKSEKLESSYMRRRLRYWR-- 305

Query: 316 RITLLQNFADAQEKEDAFDRISRFRSEGLVDPKCSNNGVMSTSRTRLLLKNEGVKGGQPV 375
             +LL+     Q+ E    ++ R + E    P  S+    S              G +PV
Sbjct: 306 --SLLE-----QDGEKDTGQLEREKVEPRAPPLFSSGSSSSFGEP---------VGPEPV 365

Query: 376 SQSRGLQEEAVLNVKSIKEFE------------------------------GVRVKEDA- 435
           S    L +   LN  +++E E                              G + +EDA 
Sbjct: 366 SPE--LVDSDTLNTSNVEEKEREGDVARKVHRYDKAMHDIGQSDRTVYGEAGKKWEEDAS 425

Query: 436 -MGANFENKSLVSSCMEEVKKRKEMEEILVREKKELERMNKEHDELLKELHDVQEQKSLL 495
              A  + K+L   C++E  +RK +EE+L +EK E++ + ++++  +KEL  VQ +   L
Sbjct: 426 TTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMVIEQNNGFMKELQMVQGRNLKL 485

Query: 496 ERKASEYRCDVEDLEK----KMFEAVDLLISFKEKRDKLQIEHEGAMNKLRQLKNMVKRE 555
           E +  +    ++DLEK    K   A++LL SF++KRD+++I+HE A+ ++  L+ +VK E
Sbjct: 486 ESQMRK----LQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHENAVKEVNALRRLVKGE 545

Query: 556 --PTCSVEMPTFSFMEIIEATRNFDPSWKIGEGRHGSVYKGFLRHMDVALKMLPSYGSHS 615
              +   EM  +SFMEI EAT  FDPSWK+GEG++GSVYKG L+H+ VA+KMLPSYGS +
Sbjct: 546 TGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQHLQVAVKMLPSYGSLN 605

Query: 616 QSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVYEHLTNSSLESHLACKDHNRPLPWQIR 675
              F+  VE+LSRVRHPNLV+++GACPESR ++Y+++ N SLE   + +++   L W+ R
Sbjct: 606 HFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESR 665

Query: 676 IRVAADICSALIFLHYSEPCIVHGDIKPSKILLDANFVAKLGGLGISRLIPQEEKAFSSD 735
           IR+A++ICSAL+FLH + PCI+HG++KPSKILLD+N V K+   GIS+LIP         
Sbjct: 666 IRIASEICSALLFLHSNIPCIIHGNLKPSKILLDSNLVTKINDYGISQLIP--------- 725

Query: 736 SAYNMSKENNPYIDPEYLETGRFTPESDVYSLGVTFLRILTGRAPSGIVKDVKCALENDK 795
              +   +++P++DP Y  +   T ESD+Y+ G+  L++LT R  SGI++DVKCALEND 
Sbjct: 726 --IDGLDKSDPHVDPHYFVSREMTLESDIYAFGIILLQLLTRRPVSGILRDVKCALENDN 785

Query: 796 ISTVLDSSAGDWPHDLAEQLALVALRCCEKEKSDRPDLVSELWSV----LEPMRSIASAS 855
           IS VLD+SAGDWP    ++LA VA+RCC+K   +RPDL   L  +       + S  ++S
Sbjct: 786 ISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDLAVVLRFIDRMKAPEVPSSETSS 804

Query: 856 FSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYEADAIRGWFKSGHDTSPMTNLKLEH 888
           +++     R P+H+ CPIFQE+MKDPLIAADGFTYEA+AIR W  +GHDTSPMTNLK+E 
Sbjct: 846 YANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMED 804

BLAST of Spg002406 vs. TAIR 10
Match: AT3G49060.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 559.3 bits (1440), Expect = 5.6e-159
Identity = 346/909 (38.06%), Postives = 510/909 (56.11%), Query Frame = 0

Query: 16  GQKLQFDVEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVAQSEFCSY 75
           G++L  DV+ETIFVAV  DV+ +K+T+LWA +NF GK  CLL+VH+ A            
Sbjct: 6   GEELVLDVDETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTAR----------- 65

Query: 76  GISFFGHVNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKALISYLPL 135
                            + W                                        
Sbjct: 66  ----------------AASW---------------------------------------- 125

Query: 136 NQSRCVGGAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQAQKVWTE 195
              + VGG          + K+H  K  E  E+ K  EL+N Y+ +L++  +Q  K+   
Sbjct: 126 THKKLVGG----------SFKKHDVKVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIA 185

Query: 196 MNSVERGIVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVCQQAPIC 255
             ++E  IVE+IA++ IKWLVMG  A SD      H+   + T LKSKKA +VC++AP  
Sbjct: 186 GQNIEECIVELIARHKIKWLVMG--AASDK-----HY-SWKMTDLKSKKAIFVCKKAPDS 245

Query: 256 CHIWFVCSGRLIYSREGRMGRFN-----PLLLQNSEIVIDQSNHLRPESVTFKLNFLED- 315
           CHIWF+C G LI++R       N     PL+  +S+    +S  L    +  +L +    
Sbjct: 246 CHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQLDSDNETRKSEKLESSYMRRRLRYWRSL 305

Query: 316 -SRITLLQNFADAQ------EKEDAFDRISRFRSEGL------------VDPKCSNNGVM 375
             ++ LLQN AD +      E+E    R     S G             V P+  ++  +
Sbjct: 306 LEQVNLLQNCADGEKDTGQLEREKVEPRAPPLFSSGSSSSFGEPVGPEPVSPELVDSDTL 365

Query: 376 STSRTRLLLKNEGVKGGQPVSQSRGLQEEAVLNVKSIKEFEGVRVKEDA--MGANFENKS 435
           +TS      +   V   + V +      +   + +++    G + +EDA    A  + K+
Sbjct: 366 NTSNVEEKEREGDV--ARKVHRYDKAMHDIGQSDRTVYGEAGKKWEEDASTTEALCKAKA 425

Query: 436 LVSSCMEEVKKRKEMEEILVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCD 495
           L   C++E  +RK +EE+L +EK E++ + ++++  +KEL  VQ +   LE +  +    
Sbjct: 426 LEGLCIKESSQRKRLEELLEKEKLEVKMVIEQNNGFMKELQMVQGRNLKLESQMRK---- 485

Query: 496 VEDLEK----KMFEAVDLLISFKEKRDKLQIEHEGAMNKLRQLKNMVKRE--PTCSVEMP 555
           ++DLEK    K   A++LL SF++KRD+++I+HE A+ ++  L+ +VK E   +   EM 
Sbjct: 486 LQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHENAVKEVNALRRLVKGETGESSGSEML 545

Query: 556 TFSFMEIIEATRNFDPSWKIGEGRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEV 615
            +SFMEI EAT  FDPSWK+GEG++GSVYKG L+H+ VA                  VE+
Sbjct: 546 DYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQHLQVA------------------VEI 605

Query: 616 LSRVRHPNLVSIIGACPESRLIVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSA 675
           LSRVRHPNLV+++GACPESR ++Y+++ N SLE   + +++   L W+ RIR+A++ICSA
Sbjct: 606 LSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESRIRIASEICSA 665

Query: 676 LIFLHYSEPCIVHGDIKPSKILLDANFVAKLGGLGISRLIPQEEKAFSSDSAYNMSKENN 735
           L+FLH + PCI+HG++KPSKILLD+N V K+   GIS+LIP            +   +++
Sbjct: 666 LLFLHSNIPCIIHGNLKPSKILLDSNLVTKINDYGISQLIP-----------IDGLDKSD 725

Query: 736 PYIDPEYLETGRFTPESDVYSLGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAG 795
           P++DP Y  +   T ESD+Y+ G+  L++LT R  SGI++DVKCALEND IS VLD+SAG
Sbjct: 726 PHVDPHYFVSREMTLESDIYAFGIILLQLLTRRPVSGILRDVKCALENDNISAVLDNSAG 785

Query: 796 DWPHDLAEQLALVALRCCEKEKSDRPDLVSELWSV----LEPMRSIASASFSSSKKHSRV 855
           DWP    ++LA VA+RCC+K   +RPDL   L  +       + S  ++S+++     R 
Sbjct: 786 DWPVARGKKLANVAIRCCKKNPMNRPDLAVVLRFIDRMKAPEVPSSETSSYANQNVPRRP 794

Query: 856 PAHFTCPIFQEIMKDPLIAADGFTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALL 888
           P+H+ CPIFQE+MKDPLIAADGFTYEA+AIR W  +GHDTSPMTNLK+E CNL+PN+AL 
Sbjct: 846 PSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALH 794

BLAST of Spg002406 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 523.9 bits (1348), Expect = 2.6e-148
Identity = 327/899 (36.37%), Postives = 483/899 (53.73%), Query Frame = 0

Query: 23  VEETIFVAVGNDVDDTKSTLLWAVQNFHGKNFCLLHVHQPAHEVVAQSEFCSYGISFFGH 82
           ++E IFVAV   V  +KSTL+WA+QN  GK  CL+HVHQP+                   
Sbjct: 43  IDEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPS------------------- 102

Query: 83  VNTASEVYALSQWLETDSDRRAPRQGATLAPRFEEARRSEEGAAKALISYLPLNQSRCVG 142
                                                             +PL  ++   
Sbjct: 103 ------------------------------------------------QMIPLMGAKFPV 162

Query: 143 GAIENEEPSSNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQAQKVWTEMNSVERG 202
           GA++ EE           + F E ER+K   +L+ Y+ I  + GV+A+K++ EM S+E G
Sbjct: 163 GAVKEEE----------VRVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENG 222

Query: 203 IVEIIAQYSIKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVCQQAPICCHIWFVC 262
           IV++I++  I+ LVMG  A+        H+  RR T LKS+KA +V ++AP  C IWF C
Sbjct: 223 IVQLISELGIRKLVMGAAAD-------RHY-SRRMTDLKSRKAIFVRREAPTLCQIWFTC 282

Query: 263 SGRLIYSREGRMGRFNPL------------LLQNSEIVIDQSNHLRPESVTFKLNFL--- 322
            G LI++RE  M                  LLQ       +  H+     T  +  L   
Sbjct: 283 KGYLIHTREATMDDTESEYASPRPSISASDLLQTFSTPESEHQHISRVQSTDSVQQLVSN 342

Query: 323 -----EDSRITLLQNFADAQEKEDAFDRI---SRFRSEGLVDPKCSNNGVMSTSRTRLLL 382
                +  R++      D +E+E     +   +   S G   P    +GV  +       
Sbjct: 343 GSSTEQSGRVSDGSLNTDEEERESDGSEVTGSATVMSSGHSSPSSFPDGVDDSF------ 402

Query: 383 KNEGVKGGQPVSQSRGLQEEAVLNVKSIKEFEGVRVKEDAMGANFENKSLVSSCMEEVKK 442
               VK  +  S++   ++EA       +     + +++A+ A    K   S+  EE+K+
Sbjct: 403 ---NVKIRKATSEAHSSKQEAF-----AETLRRQKAEKNALDAIRRAKQSESAYSEELKR 462

Query: 443 RKEMEEILVREKKELERMNKEHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEA 502
           RK+ E  + +EK+    +  E + +++EL     QK++LE + ++    +E L +K+  A
Sbjct: 463 RKDTEIAVAKEKERFITIKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIA 522

Query: 503 VDLLISFKEKRDKLQIEHEGAMNKLRQLKNMVKREPTCSVEMP----TFSFMEIIEATRN 562
           V LL   +++R++LQ E + A+ +  +L++  +   T ++++P     FSF EI EAT +
Sbjct: 523 VKLLQKLRDEREELQTERDRALREAEELRSHAE---TSTLQLPQYFTDFSFSEIEEATNH 582

Query: 563 FDPSWKIGEGRHGSVYKGFLRHMDVALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSII 622
           FD + KIGEG +GS+Y G LRH  VA+KML    S     +Q EV+VLS++RHPN++++I
Sbjct: 583 FDSTLKIGEGGYGSIYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLI 642

Query: 623 GACPESRLIVYEHLTNSSLESHLACKDHNRPLPWQIRIRVAADICSALIFLHYSEP-CIV 682
           GACPE   +VYE+L   SLE  L CKD++ PL WQ R+R+A +IC+AL+FLH ++   +V
Sbjct: 643 GACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLV 702

Query: 683 HGDIKPSKILLDANFVAKLGGLGISRLI-PQEEKAFSSDSAYNMSKENNPYIDPEYLETG 742
           HGD+KP+ ILLD+N V+KL   G   L+ P   K+  +D    ++     Y+DPE   +G
Sbjct: 703 HGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVA-----YLDPEASSSG 762

Query: 743 RFTPESDVYSLGVTFLRILTGRAPSGIVKDVKCALENDKISTVLDSSAGDWPHDLAEQLA 802
             TP+SDVYS G+  LR+LTGR    I  +VK AL+N  ++ +LD  AGDWP   AEQLA
Sbjct: 763 ELTPKSDVYSFGIILLRLLTGRPALRISNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLA 822

Query: 803 LVALRCCEKEKSDRPDLVSELWSVLEPMRSIASASFS---SSKKHSRVPAHFTCPIFQEI 862
            +ALRCCE    +RPDL +E+W VLEPMR+ +  S S      +H   P +F CPIFQE+
Sbjct: 823 RLALRCCETVSENRPDLGTEVWRVLEPMRASSGGSSSFHLGRNEHRIAPPYFICPIFQEV 834

Query: 863 MKDPLIAADGFTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEW-QHQL 889
           M+DP +AADGFTYEA+AIR W  S HDTSPMTN+KL H +L+ N+AL +AIQEW QH L
Sbjct: 883 MQDPHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEWLQHHL 834

BLAST of Spg002406 vs. TAIR 10
Match: AT5G51270.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 314.7 bits (805), Expect = 2.4e-85
Identity = 186/517 (35.98%), Postives = 302/517 (58.41%), Query Frame = 0

Query: 388 KEFEGVRVKEDAMGANFENKSLVSSCMEEVKKRKEMEEILVREKKELERMNKEHDELLKE 447
           ++F+  +    +M +NFE  ++V+         ++    + + + EL   ++ +   + +
Sbjct: 310 RDFDERKDAMSSMSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMY--AVAQ 369

Query: 448 LHDVQEQKSLLERKASEYRC---DVEDLEKKMFEAVDLLISFKEKRDKLQIEHEGAMNKL 507
           +  +   + L E K  E      + + + KK  E  +     +E+R+  Q   E  M   
Sbjct: 370 VETLDASRKLNELKFEELTLLEHETKGIAKKETEKFEQ--KRREEREAAQ-RREAEMKAT 429

Query: 508 RQLKNMVKREPTCSV----EMPTFSFMEIIEATRNFDPSWKIGEGRHGSVYKGFLRHMDV 567
            + K   K E +  V    +   F++ EII AT +F    KIG G +G VYK  L H   
Sbjct: 430 HEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIA 489

Query: 568 ALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVYEHLTNSSLESHLAC 627
           A+K+L S  S    +F  E+E+LS++RHP+LV ++GACP+   +VYE++ N SLE  L  
Sbjct: 490 AVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQ 549

Query: 628 KDHNRPLPWQIRIRVAADICSALIFLHYSEPC-IVHGDIKPSKILLDANFVAKLGGLGIS 687
            + ++P+PW +R+R+A ++ SAL+FLH S+P  I+H D+KP+ ILL+ NFV+K+G +G+S
Sbjct: 550 VNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLS 609

Query: 688 RLIPQEEKAFSSDSAYNMSKENNP-----YIDPEYLETGRFTPESDVYSLGVTFLRILTG 747
            +I   + A    + + M K+ +P     YIDPEY  TGR +P+SDVY+ G+  L++LTG
Sbjct: 610 TMI---QAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTG 669

Query: 748 RAPSGIVKDVKCALEN---DKISTVLDSSAGDWPHDLAEQLALVALRCCEKEKSDRPDLV 807
           +    +   V+ A+EN   D++  +LD  AG+WP +   QLA +AL+C E    DRPDL 
Sbjct: 670 QQAMALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLE 729

Query: 808 SELWSVLEPMRSIA-SASFSSSKKHSRVPAHFTCPIFQEIMKDPLIAADGFTYEADAIRG 867
            ++  VLE ++ +A  A  S S   S+ P+HF CP+ +++MK+P IAADG+TY+  AI  
Sbjct: 730 DQILPVLESLKKVADKARNSLSAAPSQPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEE 789

Query: 868 WFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQ 888
           W ++ H TSP+TN  L++ NL+PN+ L  AI EW+++
Sbjct: 790 WMEN-HRTSPVTNSPLQNVNLLPNHTLYAAIVEWRNR 817

BLAST of Spg002406 vs. TAIR 10
Match: AT5G61560.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 302.4 bits (773), Expect = 1.2e-81
Identity = 226/768 (29.43%), Postives = 367/768 (47.79%), Query Frame = 0

Query: 152 SNTLKEHVAKAFEEPERQKPLELLNQYVLILAKLGVQAQKVWTEMNSVERGIVEIIAQYS 211
           S T K+     +++   +K  E+L     +     VQ   +  E + +   I + +  + 
Sbjct: 52  STTRKDLTTSVYKKDVDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHG 111

Query: 212 IKWLVMGLDAESDNALHRMHFRERRPTGLKSKKAFYVCQQAPICCHIWFVCSGRLIYSRE 271
           I  LV+G    S + +     +       +S  +  +    P  C +  +  G+L+  R+
Sbjct: 112 ISELVIG---ASSSIIFSWKLK-------RSNLSSRIADATPRFCSVHVISKGKLLNVRK 171

Query: 272 GRMGRFNPLLLQNSEIVIDQSNHLRPESVTFKLNF----LEDSRITLLQNF-------AD 331
             M     +    SE      +H    S T    F    L   RI  L            
Sbjct: 172 SDMDTETSIADDRSESRFSSDSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIG 231

Query: 332 AQEKEDAFDRISRFRSEGLVDPKCSN-NGVMSTSRTRLLLKNEGVKGGQPVSQSRGLQEE 391
            Q  E      +R  S  + + K  N  G   TS + +        GG  +S  R  Q E
Sbjct: 232 KQNNEPHHHHHNRAGSLDVDESKLLNQKGFYRTSSSGI------GYGGSDISSWRSSQME 291

Query: 392 AVLNVKSIKEFEGVRVKEDAMGANFENKSLVSSCMEEVKKRKEMEEILVREKKELERMNK 451
                 S   +         +  +FE + L              +  ++   K+++ +N+
Sbjct: 292 ---EASSSSTYSDPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQ 351

Query: 452 EHDELLKELHDVQEQKSLLERKASEYRCDVEDLEKKMFEAVDLLISFKEKRDKLQIEHEG 511
              E    L ++  ++   +      R   ED E +     + +    E+R + +   E 
Sbjct: 352 RRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEE 411

Query: 512 AMNKLRQLKNMVKREPTCSVEMPTFSFMEIIEATRNFDPSWKIGEGRHGSVYKGFLRHMD 571
              + ++L++ ++  P    +   F + EI+EAT +F    KIG G +GSVY+  L H  
Sbjct: 412 VRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTT 471

Query: 572 VALKMLPSYGSHSQSRFQYEVEVLSRVRHPNLVSIIGACPESRLIVYEHLTNSSLESHLA 631
           VA+K+L S  S    +F  E+E+LS++RHP+L+ ++GACPE   +VYE++ N SLE  L 
Sbjct: 472 VAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLM 531

Query: 632 CKDHN------RPLPWQIRIRVAADICSALIFLHYSEP-CIVHGDIKPSKILLDANFVAK 691
            +  N       PL W  R R+A +I SAL FLH +EP  IVH D+KP+ ILLD N V+K
Sbjct: 532 KRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSK 591

Query: 692 LGGLGISRLIPQEEKAFSSDSAYNMSKENNP-----YIDPEYLETGRFTPESDVYSLGVT 751
           +G +G+S+++           A  +  E  P     YIDPEY  TG  TPESD+Y+ G+ 
Sbjct: 592 IGDVGLSKMV-----NLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGII 651

Query: 752 FLRILTGRAPSGIVKDVKCAL--ENDKISTVLDSSAGDWPHDLAEQLALVALRCCEKEKS 811
            L+++T R+  G+   ++ AL  +  K + +LD +AGDWP   A+++ ++ LRC E  K 
Sbjct: 652 LLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKR 711

Query: 812 DRPDLVSELWSVLEPMRSIASAS---FSSS--KKHSRVPAHFTCPIFQEIMKDPLIAADG 871
           DRPDL  E+  VLE ++ +AS +   F+ +    H   P HF CPI +++M++P +A+DG
Sbjct: 712 DRPDLGKEILPVLERLKEVASIARNMFADNLIDHHHNAPTHFYCPITKDVMENPCVASDG 771

Query: 872 FTYEADAIRGWFKSGHDTSPMTNLKLEHCNLVPNYALLNAIQEWQHQL 889
           +TYE  AI+ W +  H  SPMT+L     +L+PN++LL+AI+EW+ QL
Sbjct: 772 YTYEKRAIKEWLQKNH-KSPMTDLPFPSDSLLPNHSLLSAIKEWRSQL 794