Spg002377 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002377
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Membrane-associated kinase regulator 5
Location	scaffold6: 1083233 .. 1084311 (+)
RNA-Seq Expression	Spg002377
Synteny	Spg002377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTCTCGACTTCGTCAAGCTCCATAATCTCTACGCCCATTTCTCCTCCATTGTTGAAACTGCATATCCTCTGATTCGTTCTCATTTCTATGGCGACGATCAGGAAGAAGATTCCTTCTTCGATTTGGATTTAATCTCTCATTCTCATTCAGAAACCGGCAAATCTCCCGTCGAGAAGATCCTCACTCACTCCGGCGAAAATGAACTCTGTTTCGCTCAACGGAATCTTCTTCTCTCGCAATCCGATCTCATTTCGAAACGAAAAATCCTTCCGATTGAACTCAGTTCAAAGCCAGAGTCTCCGGTTTCCGTTTCGATTCTCAAATCGGCGCCTAAGCTCTCGATTTCCTTGTTTAAGAAGCCGAAGTTAATGGCGAAACAGAAGACGGACGAAACAGACTCTTCGTCATCGTCTCCAAAACTACAGAGCACAGAAAGCAAACGATTCGCATTCAAACTGAACAGAGTAAGCAGTTCAAGAAACAGTAATCGGATTGACGCATCTGAGCGTCAATCGAAGAGATTCTCTGGAGAAGGAATGCAGAAGTATCTGAAATTAATCAAGCCTCTATATGTGAAGGTTTCGCGGAAACATAACTACAGACCGTCGCCGGTATCTTCTCCGGCGACAGCAACGGCTACATCGATCGATGAGAAGCAGAGGAATATTGCGATAGGGATCCGAGTGGTGTGTAGGCGTCTAGGCAAGAGTAAATCATCATCATCAGCGACTGGAATGGCTGTATCGCCGACGAATAGGCGAGACGATTCTCTTTTGCAGCAAGACGACGGGATTGAGAGCGCAATTTTGCATTGCAAAAGGTCGTTCAGCGCAACAAGAGGCAAGATCCAAAACCACAAAAAACTCGAACGTCTTTTTTTTGCAATTTTTTTGTGCCCTAATTTAATTTGTTTGAAAATCTTTAGCATCTAACAATCATTTCAATCGAAATTTCAGATGATGTTGATGGAACTTGTGACGATAACCTATTCGGGACTGGCGATTTGCAGAAGGAGAAGGCGGGAAAAGATGTTTTATCAGAAGCCAAAGAAGGCGAACAAGAACGACATTGA

mRNA sequence

ATGGAAGCTCTCGACTTCGTCAAGCTCCATAATCTCTACGCCCATTTCTCCTCCATTGTTGAAACTGCATATCCTCTGATTCGTTCTCATTTCTATGGCGACGATCAGGAAGAAGATTCCTTCTTCGATTTGGATTTAATCTCTCATTCTCATTCAGAAACCGGCAAATCTCCCGTCGAGAAGATCCTCACTCACTCCGGCGAAAATGAACTCTGTTTCGCTCAACGGAATCTTCTTCTCTCGCAATCCGATCTCATTTCGAAACGAAAAATCCTTCCGATTGAACTCAGTTCAAAGCCAGAGTCTCCGGTTTCCGTTTCGATTCTCAAATCGGCGCCTAAGCTCTCGATTTCCTTGTTTAAGAAGCCGAAGTTAATGGCGAAACAGAAGACGGACGAAACAGACTCTTCGTCATCGTCTCCAAAACTACAGAGCACAGAAAGCAAACGATTCGCATTCAAACTGAACAGAGTAAGCAGTTCAAGAAACAGTAATCGGATTGACGCATCTGAGCGTCAATCGAAGAGATTCTCTGGAGAAGGAATGCAGAAGTATCTGAAATTAATCAAGCCTCTATATGTGAAGGTTTCGCGGAAACATAACTACAGACCGTCGCCGGTATCTTCTCCGGCGACAGCAACGGCTACATCGATCGATGAGAAGCAGAGGAATATTGCGATAGGGATCCGAGTGGTGTGTAGGCGTCTAGGCAAGAGTAAATCATCATCATCAGCGACTGGAATGGCTGTATCGCCGACGAATAGGCGAGACGATTCTCTTTTGCAGCAAGACGACGGGATTGAGAGCGCAATTTTGCATTGCAAAAGGTCGTTCAGCGCAACAAGAGGCAAGATCCAAAACCACAAAAAACTCGAACATGATGTTGATGGAACTTGTGACGATAACCTATTCGGGACTGGCGATTTGCAGAAGGAGAAGGCGGGAAAAGATGTTTTATCAGAAGCCAAAGAAGGCGAACAAGAACGACATTGA

Coding sequence (CDS)

ATGGAAGCTCTCGACTTCGTCAAGCTCCATAATCTCTACGCCCATTTCTCCTCCATTGTTGAAACTGCATATCCTCTGATTCGTTCTCATTTCTATGGCGACGATCAGGAAGAAGATTCCTTCTTCGATTTGGATTTAATCTCTCATTCTCATTCAGAAACCGGCAAATCTCCCGTCGAGAAGATCCTCACTCACTCCGGCGAAAATGAACTCTGTTTCGCTCAACGGAATCTTCTTCTCTCGCAATCCGATCTCATTTCGAAACGAAAAATCCTTCCGATTGAACTCAGTTCAAAGCCAGAGTCTCCGGTTTCCGTTTCGATTCTCAAATCGGCGCCTAAGCTCTCGATTTCCTTGTTTAAGAAGCCGAAGTTAATGGCGAAACAGAAGACGGACGAAACAGACTCTTCGTCATCGTCTCCAAAACTACAGAGCACAGAAAGCAAACGATTCGCATTCAAACTGAACAGAGTAAGCAGTTCAAGAAACAGTAATCGGATTGACGCATCTGAGCGTCAATCGAAGAGATTCTCTGGAGAAGGAATGCAGAAGTATCTGAAATTAATCAAGCCTCTATATGTGAAGGTTTCGCGGAAACATAACTACAGACCGTCGCCGGTATCTTCTCCGGCGACAGCAACGGCTACATCGATCGATGAGAAGCAGAGGAATATTGCGATAGGGATCCGAGTGGTGTGTAGGCGTCTAGGCAAGAGTAAATCATCATCATCAGCGACTGGAATGGCTGTATCGCCGACGAATAGGCGAGACGATTCTCTTTTGCAGCAAGACGACGGGATTGAGAGCGCAATTTTGCATTGCAAAAGGTCGTTCAGCGCAACAAGAGGCAAGATCCAAAACCACAAAAAACTCGAACATGATGTTGATGGAACTTGTGACGATAACCTATTCGGGACTGGCGATTTGCAGAAGGAGAAGGCGGGAAAAGATGTTTTATCAGAAGCCAAAGAAGGCGAACAAGAACGACATTGA

Protein sequence

MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVEKILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLFKKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGEQERH

Homology

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BLAST of Spg002377 vs. NCBI nr
Match: XP_038902999.1 (probable membrane-associated kinase regulator 5 [Benincasa hispida])

HSP 1 Score: 502.3 bits (1292), Expect = 3.2e-138
Identity = 278/328 (84.76%), Postives = 291/328 (88.72%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVKLHNLY+HFSSIVETAYPLIRSHFYGDDQ+EDSFFDLD IS   SET KSP E
Sbjct: 1   MEALDFVKLHNLYSHFSSIVETAYPLIRSHFYGDDQDEDSFFDLDFISPPPSETCKSPDE 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
             LTHSG+NELCF+QRNLLLSQSDLISK KILPIELSSKPESPVSVSILKSAP+LSISLF
Sbjct: 61  NNLTHSGQNELCFSQRNLLLSQSDLISKLKILPIELSSKPESPVSVSILKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKT+E +  S   KL STESKRFAFKLNRV+SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTEEKEPPSL--KLHSTESKRFAFKLNRVNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPA-TATATSIDEKQRNIAIGIRVVCRRLGKS 240
           GMQKYLKLIKPLYVKVSRK NYR SPVSSP+ T TATSIDEKQRNIAIGIRVVCRRLGKS
Sbjct: 181 GMQKYLKLIKPLYVKVSRKQNYRSSPVSSPSTTTTATSIDEKQRNIAIGIRVVCRRLGKS 240

Query: 241 KSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTC 300
           KSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR           D+DGTC
Sbjct: 241 KSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------DDIDGTC 300

Query: 301 DDNLFGTGDLQKEKAGKDVLSEAKEGEQ 328
           DDNLFG GDLQ +K GKD  SEAK+ EQ
Sbjct: 301 DDNLFGAGDLQNQKTGKDSFSEAKQDEQ 316

[Close]

BLAST of Spg002377 vs. NCBI nr
Match: KAG7014738.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 497.7 bits (1280), Expect = 7.9e-137
Identity = 279/328 (85.06%), Postives = 293/328 (89.33%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVKLHNLYAHFSSIVETAY L RS    D+ +EDSFFDLD +S   SETGKSP E
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYNLNRS----DNHDEDSFFDLDFVSLPTSETGKSPDE 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           K LT SGENELCF QRNLL SQSDLISKRKILPIELSSKPESPVSVSILKSAP+LSISLF
Sbjct: 61  KNLTRSGENELCFPQRNLLRSQSDLISKRKILPIELSSKPESPVSVSILKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKTDET+SSS   KLQ+TESKRFAFKLNR++SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTDETESSSL--KLQNTESKRFAFKLNRLNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
           G+QKYLKLIKPLYVKVSRK NYR SPVSSP     TSIDEKQRNIAIGIRVVCRRLGKSK
Sbjct: 181 GIQKYLKLIKPLYVKVSRKQNYRSSPVSSP----VTSIDEKQRNIAIGIRVVCRRLGKSK 240

Query: 241 -SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTC 300
            SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSA+RGKIQ  K   +DVDGTC
Sbjct: 241 SSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSASRGKIQKQKTRTYDVDGTC 300

Query: 301 DDNLFGTGDLQKEKAGKDVLSEAKEGEQ 328
           DDNLFGTGDLQK+KAG D  SEAK+GEQ
Sbjct: 301 DDNLFGTGDLQKQKAGTDAFSEAKQGEQ 318

[Close]

BLAST of Spg002377 vs. NCBI nr
Match: KAG6574213.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493.4 bits (1269), Expect = 1.5e-135
Identity = 274/326 (84.05%), Postives = 286/326 (87.73%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVKLHNLYAHFSSIVETAYPLIRS FYGDDQ+EDSFFDLD IS   S +G+SP +
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           + L+ SGE +LCF+QRNLLLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETDS  S  KLQSTESKRF FKLNRV+SSRNSNR DASERQSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDSPLSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GMQKYLKLIKPLYVKVSRK NY  S  SSP T TATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCD 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR           DVDGTCD
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------DDVDGTCD 300

Query: 301 DNLFGTGDLQKEKAGKDVLSEAKEGE 327
           DN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 DNQFGTGDLQKEKAGKDVFSEAKEGE 315

[Close]

BLAST of Spg002377 vs. NCBI nr
Match: XP_022945795.1 (probable membrane-associated kinase regulator 5 [Cucurbita moschata])

HSP 1 Score: 493.4 bits (1269), Expect = 1.5e-135
Identity = 274/326 (84.05%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVK HNLYAHFSSIVETAYPLIRS FYGDDQ+EDSFFDLD IS   S +G+SP +
Sbjct: 1   MEALDFVKFHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           + L+ SGENELCF+QRNLLLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETDS  S  KLQSTESKRF FKLNRV+SSRNSNR DASE QSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDSPLSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GMQKYLKLIKPLYVKVSRK NY  S  SSP T TATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCD 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR           DVDGTCD
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------DDVDGTCD 300

Query: 301 DNLFGTGDLQKEKAGKDVLSEAKEGE 327
           DN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 DNQFGTGDLQKEKAGKDVFSEAKEGE 315

[Close]

BLAST of Spg002377 vs. NCBI nr
Match: XP_023541341.1 (probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491.9 bits (1265), Expect = 4.3e-135
Identity = 274/326 (84.05%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVKLHNLYAHFSSIVETAYPLIRS  YGDDQ+EDSFFDLD IS   S +G+SP +
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRLYGDDQDEDSFFDLDFISPPSSGSGESPDQ 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
             L+ SGENELCF+QRNLLLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  SNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+T+ETDS SS  KLQSTESKRF FKLNRV+SSRNSNR DASERQSKRF GE
Sbjct: 121 KKPKLMAKQRTEETDSLSSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
            MQKYLKLIKPLYVKVSRK NY  S  SSP T TATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 AMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCD 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR           DVDGTCD
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------DDVDGTCD 300

Query: 301 DNLFGTGDLQKEKAGKDVLSEAKEGE 327
           DN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 DNQFGTGDLQKEKAGKDVFSEAKEGE 315

[Close]

BLAST of Spg002377 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.0e-17
Identity = 88/248 (35.48%), Postives = 131/248 (52.82%), Query Frame = 0


Query: 38  EDSFFDLDLISHSHSETGKSPVEKILTHSGENELCFAQRNLLLSQSDLISKRKILP-IEL 97
           EDSFF+L +   S S+  K+P EK    +      ++  N          K K+LP +++
Sbjct: 47  EDSFFELKI---SLSDF-KTPKEKQRLETTTTTTTYSVSN----------KSKVLPFVDI 106

Query: 98  SSKPESPVSVSILKSAPKLSISLFKK-PKLMAKQKTDETDSSSSSPKLQSTESKRFAFKL 157
           SSKP+     ++LKS  K     FKK  K    +K  E ++ +S       + +      
Sbjct: 107 SSKPQQS-PTTLLKSGQKFRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSF 166

Query: 158 NRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATA 217
            + +S   S + D        F     +++  LIKPLY K ++K +   S ++SP ++ A
Sbjct: 167 RKTASIARSQQTD-----DTMFDDSVSKRFFSLIKPLYTKSTKKQS--SSTITSPTSSPA 226

Query: 218 TSIDEKQR-NIAIGIRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHC 277
           T   EKQR NI  G+R V R+LGKS+S+S+A G  +SP NR D+SL  Q DGI+SAILHC
Sbjct: 227 TR--EKQRSNIPSGMRSVRRQLGKSRSASAAIG-GMSPANRIDESLQVQQDGIQSAILHC 269

Query: 278 KRSFSATR 283
           K+SF  +R
Sbjct: 287 KKSFHGSR 269

[Close]

BLAST of Spg002377 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 4.2e-16
Identity = 82/227 (36.12%), Postives = 118/227 (51.98%), Query Frame = 0


Query: 83  SDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLFKKPKLMAKQKTDETDSSSSSPK 142
           S+ + K+   P  LS  PES       KS   +++ + + P +    + + + +SSSS  
Sbjct: 185 SEDLDKQTHPPPPLSLSPESQ-----QKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSS 244

Query: 143 LQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNY 202
             S+ S          SS +  N  ++     KRF    MQKYLK +KPLY++VSR++  
Sbjct: 245 SSSSSS----------SSLKKQNGNESVVSDEKRF--VMMQKYLKKVKPLYIRVSRRYGE 304

Query: 203 R-------PSPVSSPATA------TATSIDEK---QRNIAIGIRVVCRRLGKSKSSSS-- 262
           +        +P S+ +TA       AT  + K     NI  G++VV + LGKS+SSSS  
Sbjct: 305 KLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTA 364

Query: 263 -------ATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGK 285
                  AT    S + RRDDSLLQQ D I+SAILHCKRSF+++R K
Sbjct: 365 TTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

[Close]

BLAST of Spg002377 vs. ExPASy TrEMBL
Match: A0A6J1G1Z9 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111449936 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 7.2e-136
Identity = 274/326 (84.05%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVK HNLYAHFSSIVETAYPLIRS FYGDDQ+EDSFFDLD IS   S +G+SP +
Sbjct: 1   MEALDFVKFHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           + L+ SGENELCF+QRNLLLSQSDLISKRKILPIELSSKPESPV VSI KSAP+LSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETDS  S  KLQSTESKRF FKLNRV+SSRNSNR DASE QSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDSPLSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GMQKYLKLIKPLYVKVSRK NY  S  SSP T TATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCD 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR           DVDGTCD
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------DDVDGTCD 300

Query: 301 DNLFGTGDLQKEKAGKDVLSEAKEGE 327
           DN FGTGDLQKEKAGKDV SEAKEGE
Sbjct: 301 DNQFGTGDLQKEKAGKDVFSEAKEGE 315

[Close]

BLAST of Spg002377 vs. ExPASy TrEMBL
Match: A0A6J1J4Q0 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111482618 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.8e-134
Identity = 271/325 (83.38%), Postives = 286/325 (88.00%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVKLHNLYAHFSSIVETAY L RSHF+GD+ +EDSFFDLD +S   SETGKSP E
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYNLNRSHFHGDNHDEDSFFDLDFVSLPTSETGKSPDE 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           K LT S ENELCF QRNLLLSQSDLISKRKILPIELSSKPESPVSVS+LKSAP+LSISLF
Sbjct: 61  KNLTRSSENELCFTQRNLLLSQSDLISKRKILPIELSSKPESPVSVSVLKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKTDET+ SS   KLQSTESKRFAFKLNR++SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTDETEPSSL--KLQSTESKRFAFKLNRLNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
           G+QKYLKLIKPLYVKVSRK NYR SPVSSP     TSIDEKQRNIAIGIRVVCRRLG+SK
Sbjct: 181 GIQKYLKLIKPLYVKVSRKQNYRSSPVSSP----VTSIDEKQRNIAIGIRVVCRRLGRSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCD 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSA+R           DVDGTCD
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSASR----------DDVDGTCD 300

Query: 301 DNLFGTGDLQKEKAGKDVLSEAKEG 326
           DNL GTGDLQK+K G D  SEAK+G
Sbjct: 301 DNLSGTGDLQKQKTGTDAFSEAKQG 309

[Close]

BLAST of Spg002377 vs. ExPASy TrEMBL
Match: A0A6J1HUJ8 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111467591 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.8e-134
Identity = 272/326 (83.44%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVKLHNLYA+FSSIVETAYPLIRS  YGDDQ+EDSFFDLD IS   S +G+SP  
Sbjct: 1   MEALDFVKLHNLYAYFSSIVETAYPLIRSRIYGDDQDEDSFFDLDFISPPSSGSGESPDR 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           + L+ SGENELCF+QRNLLLSQSDLISKRKILPIELSSKPESPV VSI KSA +LSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSASRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQ+TDETD+ SS  KLQSTESKRF FKLNRV+SSRNSNR DASERQSKRF GE
Sbjct: 121 KKPKLMAKQRTDETDTPSSL-KLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
           GM+KYLKLIKPLYVKVSRK NY  SPVSSP T TATS DEKQRNI+IGIRVVCRRLGKSK
Sbjct: 181 GMRKYLKLIKPLYVKVSRKPNYSSSPVSSPVTTTATSFDEKQRNISIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCD 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR           DVDGTCD
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------DDVDGTCD 300

Query: 301 DNLFGTGDLQKEKAGKDVLSEAKEGE 327
           DN FGT DLQKEKA KDV SEAKEGE
Sbjct: 301 DNQFGTDDLQKEKARKDVFSEAKEGE 315

[Close]

BLAST of Spg002377 vs. ExPASy TrEMBL
Match: A0A6J1EAG2 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111430826 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 7.5e-133
Identity = 273/327 (83.49%), Postives = 287/327 (87.77%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLISHSHSETGKSPVE 60
           MEALDFVKLHNLYAHFSSIVETAY L RS    D+ +EDSFFDLD +S   SETGKSP E
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYNLNRS----DNHDEDSFFDLDFVSLPTSETGKSPDE 60

Query: 61  KILTHSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLF 120
           K LT SGENELCF QRNLL SQSDLISKRKILPIELSSKPESPVSVSILKSAP+LSISLF
Sbjct: 61  KNLTRSGENELCFPQRNLLRSQSDLISKRKILPIELSSKPESPVSVSILKSAPRLSISLF 120

Query: 121 KKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGE 180
           KKPKLMAKQKTDET+S+S   KLQ+TESKRFAFKLNR++SSRN+NRIDASERQSKRFSGE
Sbjct: 121 KKPKLMAKQKTDETESASL--KLQNTESKRFAFKLNRLNSSRNNNRIDASERQSKRFSGE 180

Query: 181 GMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATATSIDEKQRNIAIGIRVVCRRLGKSK 240
           G+QKYLKLIKPLYVKVSRK NYR SPVSSP     TSIDEKQRNIAIGIRVVCRRLGKSK
Sbjct: 181 GIQKYLKLIKPLYVKVSRKQNYRSSPVSSP----VTSIDEKQRNIAIGIRVVCRRLGKSK 240

Query: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEHDVDGTCD 300
           SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSA+R           DVDGTCD
Sbjct: 241 SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSASR----------DDVDGTCD 300

Query: 301 DNLFGTGDLQKEKAGKDVLSEAKEGEQ 328
           DNLFGTGDLQK+KAG D  SEAK+GEQ
Sbjct: 301 DNLFGTGDLQKQKAGTDAFSEAKQGEQ 307

[Close]

BLAST of Spg002377 vs. ExPASy TrEMBL
Match: A0A1S3AYH4 (probable membrane-associated kinase regulator 2 OS=Cucumis melo OX=3656 GN=LOC103484123 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.1e-131
Identity = 278/334 (83.23%), Postives = 291/334 (87.13%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSHFYGDDQEEDSFFDLDLIS-HSHSETGKSPV 60
           MEALDFVKLHNLY+HFSSIVETAYPLIRSHFYGDDQ+EDSFFDLD IS    SETGKSP 
Sbjct: 1   MEALDFVKLHNLYSHFSSIVETAYPLIRSHFYGDDQDEDSFFDLDFISLPPPSETGKSPD 60

Query: 61  EKILT-HSGENELCFAQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPKLSIS 120
           EKILT HS +NEL F+QRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAP+LSIS
Sbjct: 61  EKILTVHSSQNELGFSQRNLLLSQSDLISKRKILPIELSSKPESPVSVSILKSAPRLSIS 120

Query: 121 LFKKPKLMAKQKTDETDSSSSSPKLQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFS 180
           LFKKPKLMAKQK DETDS S   KLQSTESKRFAFKLNRV+SSRN+NRIDA+ERQSKR  
Sbjct: 121 LFKKPKLMAKQKIDETDSPSH--KLQSTESKRFAFKLNRVNSSRNNNRIDATERQSKR-- 180

Query: 181 GEGMQKYLKLIKPLYVKVSRKHNYRPSPVSSPA--TATATSIDEKQRNIAIGIRVVCRRL 240
            EGMQKYLKLIKPLYVKVSRK NYR SP SSP+  T TATSIDEKQRNIAIGIRVVC+RL
Sbjct: 181 -EGMQKYLKLIKPLYVKVSRKQNYRSSPASSPSTTTTTATSIDEKQRNIAIGIRVVCKRL 240

Query: 241 GKSK---SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQNHKKLEH 300
           GKSK   SSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR           
Sbjct: 241 GKSKSSSSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR----------D 300

Query: 301 DVDGTCDDNLFGTGDLQKEKAGKDVLSEAKEGEQ 328
           DVDGTCDDN+F T DLQK+K GKD  SEAK+ EQ
Sbjct: 301 DVDGTCDDNVFVTEDLQKQKTGKDSFSEAKQDEQ 319

[Close]

BLAST of Spg002377 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 92.4 bits (228), Expect = 7.1e-19
Identity = 88/248 (35.48%), Postives = 131/248 (52.82%), Query Frame = 0


Query: 38  EDSFFDLDLISHSHSETGKSPVEKILTHSGENELCFAQRNLLLSQSDLISKRKILP-IEL 97
           EDSFF+L +   S S+  K+P EK    +      ++  N          K K+LP +++
Sbjct: 47  EDSFFELKI---SLSDF-KTPKEKQRLETTTTTTTYSVSN----------KSKVLPFVDI 106

Query: 98  SSKPESPVSVSILKSAPKLSISLFKK-PKLMAKQKTDETDSSSSSPKLQSTESKRFAFKL 157
           SSKP+     ++LKS  K     FKK  K    +K  E ++ +S       + +      
Sbjct: 107 SSKPQQS-PTTLLKSGQKFRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSF 166

Query: 158 NRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNYRPSPVSSPATATA 217
            + +S   S + D        F     +++  LIKPLY K ++K +   S ++SP ++ A
Sbjct: 167 RKTASIARSQQTD-----DTMFDDSVSKRFFSLIKPLYTKSTKKQS--SSTITSPTSSPA 226

Query: 218 TSIDEKQR-NIAIGIRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHC 277
           T   EKQR NI  G+R V R+LGKS+S+S+A G  +SP NR D+SL  Q DGI+SAILHC
Sbjct: 227 TR--EKQRSNIPSGMRSVRRQLGKSRSASAAIG-GMSPANRIDESLQVQQDGIQSAILHC 269

Query: 278 KRSFSATR 283
           K+SF  +R
Sbjct: 287 KKSFHGSR 269

[Close]

BLAST of Spg002377 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 87.0 bits (214), Expect = 3.0e-17
Identity = 82/227 (36.12%), Postives = 118/227 (51.98%), Query Frame = 0


Query: 83  SDLISKRKILPIELSSKPESPVSVSILKSAPKLSISLFKKPKLMAKQKTDETDSSSSSPK 142
           S+ + K+   P  LS  PES       KS   +++ + + P +    + + + +SSSS  
Sbjct: 185 SEDLDKQTHPPPPLSLSPESQ-----QKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSS 244

Query: 143 LQSTESKRFAFKLNRVSSSRNSNRIDASERQSKRFSGEGMQKYLKLIKPLYVKVSRKHNY 202
             S+ S          SS +  N  ++     KRF    MQKYLK +KPLY++VSR++  
Sbjct: 245 SSSSSS----------SSLKKQNGNESVVSDEKRF--VMMQKYLKKVKPLYIRVSRRYGE 304

Query: 203 R-------PSPVSSPATA------TATSIDEK---QRNIAIGIRVVCRRLGKSKSSSS-- 262
           +        +P S+ +TA       AT  + K     NI  G++VV + LGKS+SSSS  
Sbjct: 305 KLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTA 364

Query: 263 -------ATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGK 285
                  AT    S + RRDDSLLQQ D I+SAILHCKRSF+++R K
Sbjct: 365 TTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

The following BLAST results are available for this feature:

BLAST of Spg002377 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902999.1	3.2e-138	84.76	probable membrane-associated kinase regulator 5 [Benincasa hispida]	[more]
KAG7014738.1	7.9e-137	85.06	putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma...	[more]
KAG6574213.1	1.5e-135	84.05	putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma...	[more]
XP_022945795.1	1.5e-135	84.05	probable membrane-associated kinase regulator 5 [Cucurbita moschata]	[more]
XP_023541341.1	4.3e-135	84.05	probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg002377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX4	1.0e-17	35.48	Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ...	[more]
Q9SH58	4.2e-16	36.12	Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Spg002377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1Z9	7.2e-136	84.05	probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1J4Q0	1.8e-134	83.38	probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1HUJ8	6.8e-134	83.44	probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1EAG2	7.5e-133	83.49	probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A1S3AYH4	1.1e-131	83.23	probable membrane-associated kinase regulator 2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Spg002377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52870.1	7.1e-19	35.48	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G64080.1	3.0e-17	36.12	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 240..257
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 128..147
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 302..330
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 132..147
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 240..260
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 306..330
None	No IPR available	PANTHER	PTHR33929:SF4	MEMBRANE-ASSOCIATED KINASE REGULATOR 5-RELATED	coord: 1..282
IPR039619	Membrane-associated kinase regulator 2/5	PANTHER	PTHR33929	MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATED	coord: 1..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002377.1	Spg002377.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0016301	kinase activity