Spg002127 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002127
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase
Locationscaffold2: 26028780 .. 26030889 (+)
RNA-Seq ExpressionSpg002127
SyntenySpg002127
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAGCTTAATACCCACCACCGCCAAAGTGATCTCAGCCCATTTTTCAAGCCATTTTGCTCATCTTTTTCCACCCAAAATTTCAGAGAACTTGAGGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTTGAGGTTGAAGAAGATAAGAAAAGCTCATGCAAAGCCAACCATAGCAGAATCTGGGTGCAAAACTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACAGAAGGGAATAAGAGGTGAGATTTTATTTTCTTGAAAGTTAGTTCATTTTCTAACAAAATTCATGAAGGAAGCTTGAGCTAAATCGTATGAGAAGTTAATGGATTTTTGAATGGAAAACAGAGCACAGGATTTTCGAGCAAACCAGGAGGATCTCTGGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTCGAATCAGAAGCTATATTTGGTATGGTTGGAAAGCTTATTAAATTTCCTACAACGTCCATGAAGATTTTGTGAGCCAAAAATGACTTTAAGTAGGGTTAATTTCAGGAGTTAGATAAAGGGTAGCAGAGAACCTCGAACCTGTGTTGTCTGTGTTCGGGGCAATTCTGGGCAAATCGGTTGGGAATCGCATTTTCATTTCTTCAGGTAAGTTGTAGAGTGTTCAAAAATGAAGAGTTTGATGTGTATTTCATTAGTTTTGGTTAAGTATTGAGGAAGTTATGGGTGTTGGAAGTTAGGGTATAGAATCTGGAAATCTGGAAAGTAGAATGTGAATGTGATTCTTAATCCAATTGTTTAAAAAATCATGAGCATGGAAAGACAAAGCTATCTATGGTTCTAATGCTCATTTTTGGTTATTTGACAGGCCAAGAGGGAATAGGTCGAGTCTACAAACTGGAGAACTAGATAAGACAGTGAGTGACAAAATGAATTTCGAAAATTGTTTCCAATGGTTGTTCTTGTTTAGACGTTTTATACGCTTTTCTTTGAATGATACATGATTTCTATGAACGTTTTCAAGCATAAGTTTTGAGCTCAGATTTTCTAATGAGGTTTATATACGAGCACGTGGCTAATGACTTTATGAAAAGTAGCTGAAACGATATCTTTTAAAGTAAAGTATGGATTAACAAGTACTTTTAAACGATTTCCAGTTTTTCACGAGCAAGCTGAGTAAGCTTAAGTTTTACGAAAGATAACGATAAGCAGACATGTTTTAATGTTTTCATGTGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGTGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAATGTTTGATGTACTTAATATGCCTTGCTAGATTTTTAATGACAAACTTGCGAGATGTTTTGGAAAGCCTTTATTATTACACGTTTATGTTAAACGCTTTAATTGAGCATATTTATACGTTTAAAGGTTTATCACGTGTTTTGACTTCCGGATTTAGAGTTTTAAAGTTAGCCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTTCCACTTTCAGGTAGAGATCGAGCTCCCGGTGCTTGATACACTGCCAGCGTCTGCTGAAAGTTCCAGAGCAAGCTCTAGTACGTGGGTGGAGTTGTATATTGAGTATGTTCATATTGTAAATGTTTTGTAAGGATTGGATAATATCGTCCTAGAGGTCGTGGTTGTATAAACCCTAGTTGTATATGTTTTGGTCGTGATATCTTGTCAGGGCGGCACCTCGTTTTTGTTGAAAGTTTTTTTCAGGGATTCCGCTGTGTTCTGTTTCATGTAGAATGGTTTATATCTATGATTAGTATGTTTATCAGGTTCAACGTTCAGGAGGGTCAGCGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGGCTAAGCTAGCAGGTGGTCCAGGAGGGGGTGTGACAATTTTATACCGCAAGCGTACGGGTCGTCACAAGTAA

mRNA sequence

ATGTTAGTAGAGATCGAGCTCCCGGTGCTTGATACACTGCCAGCGTCTGCTGAAAGTTCCAGAGCAAGCTCTAGTACGTGGGTGGAGTTGTATATTGAGTTCAACGTTCAGGAGGGTCAGCGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGGCTAAGCTAGCAGGTGGTCCAGGAGGGGGTGTGACAATTTTATACCGCAAGCGTACGGGTCGTCACAAGTAA

Coding sequence (CDS)

ATGTTAGTAGAGATCGAGCTCCCGGTGCTTGATACACTGCCAGCGTCTGCTGAAAGTTCCAGAGCAAGCTCTAGTACGTGGGTGGAGTTGTATATTGAGTTCAACGTTCAGGAGGGTCAGCGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGGCTAAGCTAGCAGGTGGTCCAGGAGGGGGTGTGACAATTTTATACCGCAAGCGTACGGGTCGTCACAAGTAA

Protein sequence

MLVEIELPVLDTLPASAESSRASSSTWVELYIEFNVQEGQRVSLEEDDVRWLHAIFRAKLAGGPGGGVTILYRKRTGRHK
Homology
BLAST of Spg002127 vs. NCBI nr
Match: TYK27998.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 89.7 bits (221), Expect = 1.2e-14
Identity = 46/69 (66.67%), Postives = 55/69 (79.71%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIEFNVQEGQRVS--LEEDDVRWLHAIFRAK 61
           +VEIELPV DTLP SAESS ++SSTW+ELY+EF   E   ++  + EDDV WLHA+FRAK
Sbjct: 33  VVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVREDDVCWLHAVFRAK 92

Query: 62  LAGGPGGGV 69
            AGGPGGGV
Sbjct: 93  TAGGPGGGV 101

BLAST of Spg002127 vs. NCBI nr
Match: KAA0066851.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 89.7 bits (221), Expect = 1.2e-14
Identity = 46/69 (66.67%), Postives = 55/69 (79.71%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIEFNVQEGQRVS--LEEDDVRWLHAIFRAK 61
           +VEIELPV DTLP SAESS ++SSTW+ELY+EF   E   ++  + EDDV WLHA+FRAK
Sbjct: 33  VVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVREDDVCWLHAVFRAK 92

Query: 62  LAGGPGGGV 69
            AGGPGGGV
Sbjct: 93  TAGGPGGGV 101

BLAST of Spg002127 vs. NCBI nr
Match: KAA0037581.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 88.2 bits (217), Expect = 3.5e-14
Identity = 51/95 (53.68%), Postives = 60/95 (63.16%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIE-FNVQEGQRVSLEE-------------- 61
           LVEIELPV D LP SAESSR++SSTW+ELY E  +V+   +V L+               
Sbjct: 146 LVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILWSAWIV 205

Query: 62  --DDVRWLHAIFRAKLAGGPGGGVTILYRKRTGRH 80
             DDV WLHAIFRAK AGGPGGGVT  Y+ +   H
Sbjct: 206 RGDDVCWLHAIFRAKTAGGPGGGVTTWYQSQESCH 240

BLAST of Spg002127 vs. NCBI nr
Match: TYK03091.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 88.2 bits (217), Expect = 3.5e-14
Identity = 51/95 (53.68%), Postives = 60/95 (63.16%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIE-FNVQEGQRVSLEE-------------- 61
           LVEIELPV D LP SAESSR++SSTW+ELY E  +V+   +V L+               
Sbjct: 146 LVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILWSAWIV 205

Query: 62  --DDVRWLHAIFRAKLAGGPGGGVTILYRKRTGRH 80
             DDV WLHAIFRAK AGGPGGGVT  Y+ +   H
Sbjct: 206 RGDDVCWLHAIFRAKTAGGPGGGVTTWYQSQESCH 240

BLAST of Spg002127 vs. NCBI nr
Match: KAA0062409.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 84.3 bits (207), Expect = 5.1e-13
Identity = 47/91 (51.65%), Postives = 54/91 (59.34%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIE---------------------FNVQEGQ 61
           +VEIELPV DTLP SAESS ++SSTW+ELY E                     F+V  G 
Sbjct: 93  VVEIELPVPDTLPTSAESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGS 152

Query: 62  RVSLEEDDVRWLHAIFRAKLAGGPGGGVTIL 72
              +  DDV WLHA+FRAK  GGPGGGVT L
Sbjct: 153 TGIVRGDDVCWLHAVFRAKTVGGPGGGVTTL 183

BLAST of Spg002127 vs. ExPASy TrEMBL
Match: A0A5D3DX39 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G002140 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.8e-15
Identity = 46/69 (66.67%), Postives = 55/69 (79.71%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIEFNVQEGQRVS--LEEDDVRWLHAIFRAK 61
           +VEIELPV DTLP SAESS ++SSTW+ELY+EF   E   ++  + EDDV WLHA+FRAK
Sbjct: 33  VVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVREDDVCWLHAVFRAK 92

Query: 62  LAGGPGGGV 69
            AGGPGGGV
Sbjct: 93  TAGGPGGGV 101

BLAST of Spg002127 vs. ExPASy TrEMBL
Match: A0A5A7VMG9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G002220 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.8e-15
Identity = 46/69 (66.67%), Postives = 55/69 (79.71%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIEFNVQEGQRVS--LEEDDVRWLHAIFRAK 61
           +VEIELPV DTLP SAESS ++SSTW+ELY+EF   E   ++  + EDDV WLHA+FRAK
Sbjct: 33  VVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVREDDVCWLHAVFRAK 92

Query: 62  LAGGPGGGV 69
            AGGPGGGV
Sbjct: 93  TAGGPGGGV 101

BLAST of Spg002127 vs. ExPASy TrEMBL
Match: A0A5D3BTP3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00020 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.7e-14
Identity = 51/95 (53.68%), Postives = 60/95 (63.16%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIE-FNVQEGQRVSLEE-------------- 61
           LVEIELPV D LP SAESSR++SSTW+ELY E  +V+   +V L+               
Sbjct: 146 LVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILWSAWIV 205

Query: 62  --DDVRWLHAIFRAKLAGGPGGGVTILYRKRTGRH 80
             DDV WLHAIFRAK AGGPGGGVT  Y+ +   H
Sbjct: 206 RGDDVCWLHAIFRAKTAGGPGGGVTTWYQSQESCH 240

BLAST of Spg002127 vs. ExPASy TrEMBL
Match: A0A5A7T7M6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002250 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.7e-14
Identity = 51/95 (53.68%), Postives = 60/95 (63.16%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIE-FNVQEGQRVSLEE-------------- 61
           LVEIELPV D LP SAESSR++SSTW+ELY E  +V+   +V L+               
Sbjct: 146 LVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILWSAWIV 205

Query: 62  --DDVRWLHAIFRAKLAGGPGGGVTILYRKRTGRH 80
             DDV WLHAIFRAK AGGPGGGVT  Y+ +   H
Sbjct: 206 RGDDVCWLHAIFRAKTAGGPGGGVTTWYQSQESCH 240

BLAST of Spg002127 vs. ExPASy TrEMBL
Match: A0A5A7V9N3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002110 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.5e-13
Identity = 47/91 (51.65%), Postives = 54/91 (59.34%), Query Frame = 0

Query: 2   LVEIELPVLDTLPASAESSRASSSTWVELYIE---------------------FNVQEGQ 61
           +VEIELPV DTLP SAESS ++SSTW+ELY E                     F+V  G 
Sbjct: 93  VVEIELPVPDTLPTSAESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGS 152

Query: 62  RVSLEEDDVRWLHAIFRAKLAGGPGGGVTIL 72
              +  DDV WLHA+FRAK  GGPGGGVT L
Sbjct: 153 TGIVRGDDVCWLHAVFRAKTVGGPGGGVTTL 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK27998.11.2e-1466.67reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0066851.11.2e-1466.67reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0037581.13.5e-1453.68reverse transcriptase [Cucumis melo var. makuwa][more]
TYK03091.13.5e-1453.68reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0062409.15.1e-1351.65reverse transcriptase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3DX395.8e-1566.67Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... [more]
A0A5A7VMG95.8e-1566.67Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5D3BTP31.7e-1453.68Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37... [more]
A0A5A7T7M61.7e-1453.68Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5A7V9N32.5e-1351.65Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold15... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002127.1Spg002127.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity