Spg002030 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002030
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionBRO1 domain-containing protein
Locationscaffold10: 836532 .. 841009 (+)
RNA-Seq ExpressionSpg002030
SyntenySpg002030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGACTTCTACGTCTCCGCCATGTGAGATTATGTCTCGGCCTCTGCACCTTCCTATTCCCAGATTCTCTCTCTCTTTCTCTTTCTCTTTCTCTTTCTTTCTGTGCTTTTGATTCTTATCAGATCCTTTCTAATGCTCCCCAGCGCCACAACCTTCTCTGCTCTCTCTCCTCTCTGTAACCAGTAAGTCTTTTCTGTTTCTCTGACACTTTCTTTTTTATTTTTCCATCCAACTTGTGCTCTCTCTTATCTGTTTTTTCTATCTGTCTCCTTAATCATTCATTCCATTTCCAACGATTTCTTTTCTAATTTTTTAAACTATTTCTTCTATTGTTGTACTGCTAATCGTCTGATTCCTTGCCCCTTTTGTGTCTACTTCACATTCCTCACTCACTCCATCATAACCCACCTCTTGATTTGGTGCTCAATGCCTTCTTCCATGGTCAACTCTTGTAATCTGTTTAAATGGGGCTCAAATTCCATGTGGGGTTTCTTTGATTTTGTGTAGGATGTTAAGTTTGGGGCAGTTTCCCACCGAGAAGAATGGGTTGCACTTCTTCTGTTTACGCTGTTGGGAGGAAGAAGAAACTGAGCATCCCGGAGATGGTTGTGTTTGTTCCTTCCATGCGAATTCCAGTGCAATCTGATCTTCAGAGGCCTCTTAGAGGGCTTATTTCAAAAGATCTTGCTGATAGACTCTCTTCACTTCGCAATCAGATTGTGTTGGTAGCAGAAGATACAGGTCTGATTCTGGTCTTTAATCTCTTCTGCTTACTGTTACTGCGTTAATTCCTGTTTGGTTACTGGAAAAAGAAAAATTAGTTGTTCTTGTTTGGTTCTTGGCCATTTTGGACATCCCAGATTTTCGTTTCTGTTATTTATAAATATGATTTGTTTTGTAATGGTGACTCCAAATATTAATATCCTGCTAGTTTCTTTGGCAACTAAATGTTATAGGATGAAACGGTAGTTTTATGAGATTAGTTGAGGTGTGTTCAAGTTGGCAAGGACACTAACAGATATAGAAAATGTTTGTTTTGTAATGAAATGAATCCTGTTCTTACAGGTGGATCTGCTATTACGGAATTGCGACGCGCTCTCGAGGAGTATCTATCTGTTTTAATTGGTCTAACAAAGAGAGGTATCTTCATCTTTCTCATCCTACAGTTATACTCTGTCTCTGTCTCTGTCTCATGGATGATAGTCCCAATTTTATGCAGAAACCATTGATGATGGATTAATAGAGTTCAGATGGAGAAATTTAGAAAATGGACGACAAGTAAGATTGGCGCCTGCATTCATACTTTATGTTGTTTACTTATCCATAATCTGCTGTCATGTAATTGAGTTTCAAATAACAAACATGGTGGGCAGGAAATATGTGTAGCAAACTTCTGGTTTGAATTGCTATCTATCGTTCACTTAATGGCTATGCTGACTCTATCAGAAGCTGATTCCTTGATGATCCCCAAGGATCACTCTGGTTCTGGTTTGAGAGTAGTGTCTTCAGGTCATATTTACAAGCCTATTTTTCGATACTTATCATCTCGAAAACGACTATAACGGTCCACCTATTAACTAAATATGACTTAACTTTCAGATTCCAAGAGGGATGCCATTGATCTACTTCTTAAAGCATCGGGGTATCTCGATTGCTGCATCCGGGATATTCTGGTCTACATTCCATTTGATATTAAGTATGCGAGCTGCAGCCCTTTTAAATCTTTCCAAGTAGACTTGTGCTGGAGTATCTTTCTCTAACATTATCTTCATATGTATCCTAGGAAGCGATTGCCTAAAGATTTTCAACCTGGTGTGCTTGAAGCCATTTCAATTCAAACTTTGGGTCAGGTACAATGTCGTTGTCGTCCTATCGATATAGAGTGGACGTGTCTATCTCAAGTTTTTCAATCTATATCTTACATGAATGGTTTATGGTTTTTTGGTATCTATATCTTACATGAATGGAGGTATCTGCTTATAATCAGGGGACTGAAATCCAACTTGGTTTAGCTGTTGAAAGTCAAAATGCCACTTTATCTGTTAAGAGGAGATTGGCATGTGAGCAATTGATCTATTTCAGCCAGGTTTGAGTGACTTTATCTTGCATTACTGATTAGTCATCCTTTCCCCAAATGTGAAGAACATTGCCTAACTTTTGTTGTGGTATCAATCTCCCTAGTTTTGCCTTTCCTTGGAGTTTGATGTGTTGAGTATTTTGTTTGTCAGGCTTACCAGTGCCTATCGGAATGCGACACGGACCGAGGTTATGGGAAGAAGCACATGTCTTTCATCAAATGGAAATTTCTTGAAGCCAAGGTTCTTGCATAGTTGTTATCAAAATGTGCTATTGAACCTATGTTGTTTCTGCATTTCTCAAACATATTGTTGCATCAGGCTGCGGCTTACTACTACCACGGTCTGATCCTCGACAAGGGTTCGGAACCGTCGTGCCATGTCAGTGCTGTGTGCTGTTTTCTTGCTGCAGAAGAGCTTCTGCTTGAAAGCAAGAAGGCCTGCTTGAGCTTCTGCCTTGCATCCCCTGTGACCAGGTTGGGTTGCTTTCATACTTCTCAAGTAGAACATTCTCCTCTCCTTTTTGGATTCTGCTGCTCTAATTCTATCAGTTCAAAACTTGAATACTCAATTTTCATAATGCTTTTTTTTTTTTTTTTTTTTTAAATTTTGCAAGATCTCCTCCACTTTGGGGTGCTATGAAACATTTACATCAGAAAATCCCAGAGGTTGCTTCTAGGAAGTCTCAGATGTATGGATACCTCTTGGAACAAGAGAAGTAAGACATCTTTACTTATTACCTCCTTTTTGCAATTTGATACATATATAAACTTGTTCACTTTATCTTTAGTTATTTTTTGCTCAAATTTCAAAGTCTTTTAACTTATATAGTTTGGAGTTGAAACGTCTCGTTCTATGTAATTTTTTCTTTTTTTTTAATATGTGGGATGAGAGGATTTGAACCACAGTCCTCTCGGTCATTAGTACAAGTTCTATGCCAGTTGAGCTATGCTCATGTTGGCTCGTTCCATGTGATCTTGTGTCTTCTAGGGTGCAAGACATGTTAAAGTAGGATAGCCCTCTAAAACCTAAGCACGAAGTACTTAAAACAAAGTGAGGGTTTTGTTTTTTGTGAAGAGTAGAATAAAAAAGTTGTAAATATACATTTTATGTAAAAAAAAAAAAAGTATAGTAGTATAAGAAAATTTTAAGAAATGAGAGTTTTTTTTTTAAGGTTTAAATCCTATTTTAGTTCCTAAACTTTTAAGTTTATTCTATTTTAGTCCCCTAACTTTTAAAACATCCAATTTAGTCCCTAAACTTTCAAGTTTGTTATATATTGGTCCCGAGACTTTTAAAATGTTTATTTTAGTCCTTGAACTTTCAACAACAAATAATTAAAAAAAAAATCAACAACAACTATTTTGGTCTTCACTTTTATTTCAGTTTTAACAAATTAAAGACGTAGCTTTCGGTATGTGTATTAACATGTTAATATGAGCTTGATTTCAAGCCAAATAAGTTAGAGATAAATTTGAGCAAAGTGAATTTAAGGTAGAAAAGTAGTATGGAATAAACTTGAAAGTTTAAGGACCAAAATGGATATTTTGAAAGTTCAGGGGCCAAAATAGACCAAACTTGAAAGTTTGGGGACTAAAATGAGATTTAAACCTTTTTTAATAAGAGATTGGATAAAGAAATGAAAAGTTTTGAAAAATATTAAGTAATTTTTTAAAAACATTTTAAAAAATTAAGTAGGTTGAGACTAGGGTATGTGCTTTCTTATTCGTGCTAGGGAGCCTTGCTTAACAAGGCACCCCTAAGACGTCTTCTCAAAAAACATTTAGGCATGTGATTTGAAAACACCCATGTTAGTATTTGGATTATATAGAAAAATGTCGCAAAAACTTAGTTTATATATAGTTTGTGTTTGGTTTTTGAATTAGTGATTTTTAGAAAAAGATGTGACCTACTACGATACATGCACTTTTAAAACCTTGAATTTATCATGAAACTTCGTAGTCGAATAAACAAAGTCAAGTATCAAGATTTAGTTTGATGACCATAGTTCTTATTCTTCAAACAAATAGTACATATTTGAAAAAGGAAGCATAGGGTTAAGCCTAAAATAGTATTTAAATGTTATATTTTATGAAAATATAAGTTTGAATATAAAGTAATGAAACTTTGATGGACTGTGACAAACAGGGCCCTACAAGCATTACCTGAATTACCAGAATTTCAACTGTCACTAAGGCCAGAGGATTATCAACTGCCTGAAGTTGACTCAGCTTGGAGCTCTGAAAATTGGGAAGCACAGAATCAGACCTTAAAACAACACCTTAATGACAGTGATTATGAGACCATGTCTGAATGACTTCTTTTAATCTTTTCTGCTTTATTATTATTTTTTTCTTTTCTTCTGTAACCTAAAAATATATCAACATCACTGATTTTGGGTATA

mRNA sequence

ATGGGTTGCACTTCTTCTGTTTACGCTGTTGGGAGGAAGAAGAAACTGAGCATCCCGGAGATGGTTGTGTTTGTTCCTTCCATGCGAATTCCAGTGCAATCTGATCTTCAGAGGCCTCTTAGAGGGCTTATTTCAAAAGATCTTGCTGATAGACTCTCTTCACTTCGCAATCAGATTGTGTTGGTAGCAGAAGATACAGGTGGATCTGCTATTACGGAATTGCGACGCGCTCTCGAGGAGTATCTATCTGTTTTAATTGGTCTAACAAAGAGAGAAACCATTGATGATGGATTAATAGAGTTCAGATGGAGAAATTTAGAAAATGGACGACAAGAAATATGTGTAGCAAACTTCTGGTTTGAATTGCTATCTATCGTTCACTTAATGGCTATGCTGACTCTATCAGAAGCTGATTCCTTGATGATCCCCAAGGATCACTCTGGTTCTGGTTTGAGAGTAGTGTCTTCAGATTCCAAGAGGGATGCCATTGATCTACTTCTTAAAGCATCGGGGTATCTCGATTGCTGCATCCGGGATATTCTGGTCTACATTCCATTTGATATTAAGAAGCGATTGCCTAAAGATTTTCAACCTGGTGTGCTTGAAGCCATTTCAATTCAAACTTTGGGTCAGGGGACTGAAATCCAACTTGGTTTAGCTGTTGAAAGTCAAAATGCCACTTTATCTGTTAAGAGGAGATTGGCATGTGAGCAATTGATCTATTTCAGCCAGGCTTACCAGTGCCTATCGGAATGCGACACGGACCGAGGTTATGGGAAGAAGCACATGTCTTTCATCAAATGGAAATTTCTTGAAGCCAAGGCTGCGGCTTACTACTACCACGGTCTGATCCTCGACAAGGGTTCGGAACCGTCGTGCCATGTCAGTGCTGTGTGCTGTTTTCTTGCTGCAGAAGAGCTTCTGCTTGAAAGCAAGAAGGCCTGCTTGAGCTTCTGCCTTGCATCCCCTGTGACCAGGTTGGGTTGCTTTCATACTTCTCAAAAAATCCCAGAGGTTGCTTCTAGGAAGTCTCAGATGTATGGATACCTCTTGGAACAAGAGAAGGCCCTACAAGCATTACCTGAATTACCAGAATTTCAACTGTCACTAAGGCCAGAGGATTATCAACTGCCTGAAGTTGACTCAGCTTGGAGCTCTGAAAATTGGGAAGCACAGAATCAGACCTTAAAACAACACCTTAATGACAGTGATTATGAGACCATGTCTGAATGA

Coding sequence (CDS)

ATGGGTTGCACTTCTTCTGTTTACGCTGTTGGGAGGAAGAAGAAACTGAGCATCCCGGAGATGGTTGTGTTTGTTCCTTCCATGCGAATTCCAGTGCAATCTGATCTTCAGAGGCCTCTTAGAGGGCTTATTTCAAAAGATCTTGCTGATAGACTCTCTTCACTTCGCAATCAGATTGTGTTGGTAGCAGAAGATACAGGTGGATCTGCTATTACGGAATTGCGACGCGCTCTCGAGGAGTATCTATCTGTTTTAATTGGTCTAACAAAGAGAGAAACCATTGATGATGGATTAATAGAGTTCAGATGGAGAAATTTAGAAAATGGACGACAAGAAATATGTGTAGCAAACTTCTGGTTTGAATTGCTATCTATCGTTCACTTAATGGCTATGCTGACTCTATCAGAAGCTGATTCCTTGATGATCCCCAAGGATCACTCTGGTTCTGGTTTGAGAGTAGTGTCTTCAGATTCCAAGAGGGATGCCATTGATCTACTTCTTAAAGCATCGGGGTATCTCGATTGCTGCATCCGGGATATTCTGGTCTACATTCCATTTGATATTAAGAAGCGATTGCCTAAAGATTTTCAACCTGGTGTGCTTGAAGCCATTTCAATTCAAACTTTGGGTCAGGGGACTGAAATCCAACTTGGTTTAGCTGTTGAAAGTCAAAATGCCACTTTATCTGTTAAGAGGAGATTGGCATGTGAGCAATTGATCTATTTCAGCCAGGCTTACCAGTGCCTATCGGAATGCGACACGGACCGAGGTTATGGGAAGAAGCACATGTCTTTCATCAAATGGAAATTTCTTGAAGCCAAGGCTGCGGCTTACTACTACCACGGTCTGATCCTCGACAAGGGTTCGGAACCGTCGTGCCATGTCAGTGCTGTGTGCTGTTTTCTTGCTGCAGAAGAGCTTCTGCTTGAAAGCAAGAAGGCCTGCTTGAGCTTCTGCCTTGCATCCCCTGTGACCAGGTTGGGTTGCTTTCATACTTCTCAAAAAATCCCAGAGGTTGCTTCTAGGAAGTCTCAGATGTATGGATACCTCTTGGAACAAGAGAAGGCCCTACAAGCATTACCTGAATTACCAGAATTTCAACTGTCACTAAGGCCAGAGGATTATCAACTGCCTGAAGTTGACTCAGCTTGGAGCTCTGAAAATTGGGAAGCACAGAATCAGACCTTAAAACAACACCTTAATGACAGTGATTATGAGACCATGTCTGAATGA

Protein sequence

MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIVLVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWFELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDILVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLIYFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCCFLAAEELLLESKKACLSFCLASPVTRLGCFHTSQKIPEVASRKSQMYGYLLEQEKALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQNQTLKQHLNDSDYETMSE
Homology
BLAST of Spg002030 vs. NCBI nr
Match: XP_038877385.1 (BRO1 domain-containing protein BROX homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 745.3 bits (1923), Expect = 2.7e-211
Identity = 382/417 (91.61%), Postives = 395/417 (94.72%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLI  DL  RLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLIPNDLVHRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELRRALEEYLSVLIGLTK+ETIDDGLIEF+WRNLENGRQE CVANFWF
Sbjct: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKKETIDDGLIEFKWRNLENGRQETCVANFWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS+VHLMAMLTLSEADSLMIPKDH+GSGLRVVSSD KRDAIDLL+KASGYLDCCIRDI
Sbjct: 121 ELLSVVHLMAMLTLSEADSLMIPKDHTGSGLRVVSSDCKRDAIDLLIKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LVYIPFDIKKRLP DFQPG+LEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI
Sbjct: 181 LVYIPFDIKKRLPNDFQPGMLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECD DRGYGKKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCH+SAVCC
Sbjct: 241 YFSQAYQCLSECDMDRGYGKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHISAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELLLESKKACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLLESKKACLSFCLASPVTRSPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQNQT--LKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+DSAWSSEN EAQ+QT  LK+HLNDSDYET+SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPELDSAWSSENCEAQSQTLKLKEHLNDSDYETLSE 417

BLAST of Spg002030 vs. NCBI nr
Match: XP_022935442.1 (BRO1 domain-containing protein BROX homolog isoform X1 [Cucurbita moschata] >XP_022935443.1 BRO1 domain-containing protein BROX homolog isoform X1 [Cucurbita moschata] >KAG7017858.1 hypothetical protein SDJN02_19724 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 734.9 bits (1896), Expect = 3.7e-208
Identity = 377/416 (90.62%), Postives = 396/416 (95.19%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMV+FVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVMFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELRR LEEYLSVLI LTK+ETIDDGLIEF+WRNLE+GRQEICVANFWF
Sbjct: 61  LVAEDTGGSAITELRRVLEEYLSVLICLTKKETIDDGLIEFKWRNLEHGRQEICVANFWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS++HLMAMLTLSEADSLMIPKD +GSGLRVVSSD KR+AIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVLHLMAMLTLSEADSLMIPKDPTGSGLRVVSSDCKREAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LV+IPFDIKKRLPK FQPG LEA+SIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQL+
Sbjct: 181 LVHIPFDIKKRLPKSFQPGALEAMSIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLV 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECDTDRGY KKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDTDRGYEKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELLLESK+ACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLLESKRACLSFCLASPVTRSPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVD-SAWSSENWEAQNQTLKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+D SAWSSE+ EAQ+QTLK+HLNDSDYET+SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSSAWSSEHCEAQSQTLKEHLNDSDYETLSE 416

BLAST of Spg002030 vs. NCBI nr
Match: XP_038877386.1 (BRO1 domain-containing protein BROX homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 734.6 bits (1895), Expect = 4.8e-208
Identity = 379/417 (90.89%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLI  DL  RLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLIPNDLVHRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELRRALEEYLSVLIGLTK+   DDGLIEF+WRNLENGRQE CVANFWF
Sbjct: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKK---DDGLIEFKWRNLENGRQETCVANFWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS+VHLMAMLTLSEADSLMIPKDH+GSGLRVVSSD KRDAIDLL+KASGYLDCCIRDI
Sbjct: 121 ELLSVVHLMAMLTLSEADSLMIPKDHTGSGLRVVSSDCKRDAIDLLIKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LVYIPFDIKKRLP DFQPG+LEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI
Sbjct: 181 LVYIPFDIKKRLPNDFQPGMLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECD DRGYGKKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCH+SAVCC
Sbjct: 241 YFSQAYQCLSECDMDRGYGKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHISAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELLLESKKACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLLESKKACLSFCLASPVTRSPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQNQT--LKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+DSAWSSEN EAQ+QT  LK+HLNDSDYET+SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPELDSAWSSENCEAQSQTLKLKEHLNDSDYETLSE 414

BLAST of Spg002030 vs. NCBI nr
Match: XP_023527953.1 (BRO1 domain-containing protein BROX homolog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 734.6 bits (1895), Expect = 4.8e-208
Identity = 376/416 (90.38%), Postives = 396/416 (95.19%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMV+FVPS+RIPVQSDLQRPLRGLISKDLADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVMFVPSIRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELRR LEEYLSVLI LTK+ETIDDGLIEF+WRNLE+GRQEICVANFWF
Sbjct: 61  LVAEDTGGSAITELRRVLEEYLSVLICLTKKETIDDGLIEFKWRNLEHGRQEICVANFWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS++HLMAMLTLSEADSLMIPKD +GSGLRVVSSD KR+AIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVLHLMAMLTLSEADSLMIPKDPTGSGLRVVSSDCKREAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LV+IPFDIKKR+PK FQPG LEA+SIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQL+
Sbjct: 181 LVHIPFDIKKRMPKSFQPGALEAMSIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLV 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECDTDRGY KKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDTDRGYEKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELLLESK+ACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLLESKRACLSFCLASPVTRSPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVD-SAWSSENWEAQNQTLKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+D SAWSSEN EAQ+QTLK+HLNDSDYET+SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSSAWSSENCEAQSQTLKEHLNDSDYETLSE 416

BLAST of Spg002030 vs. NCBI nr
Match: KAA0060334.1 (BRO1 domain-containing protein BROX-like protein isoform X1 [Cucumis melo var. makuwa] >TYK22083.1 BRO1 domain-containing protein BROX-like protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 734.6 bits (1895), Expect = 4.8e-208
Identity = 378/417 (90.65%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIP+QSDLQRPLRGLI KDLADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPMQSDLQRPLRGLILKDLADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELR ALEEYLSVLIGLTK+ETIDDGLIEF+WRNLENGRQE C+AN WF
Sbjct: 61  LVAEDTGGSAITELRGALEEYLSVLIGLTKKETIDDGLIEFKWRNLENGRQETCIANLWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS+VHLMAMLTLSEADSLMIPKDHSGSG RVVSSDSKRDAIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVVHLMAMLTLSEADSLMIPKDHSGSGSRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LVYIPFDIKKRLP DF+PGVLEAISIQTLGQGTEIQLGLAVE+QNATLSVKRRLACEQLI
Sbjct: 181 LVYIPFDIKKRLPHDFKPGVLEAISIQTLGQGTEIQLGLAVETQNATLSVKRRLACEQLI 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECD DRGYGKKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDMDRGYGKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELL ESKKACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLGESKKACLSFCLASPVTRPPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQNQT--LKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+DSAWS +N+EA + T  LK+HLNDSDYET SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSAWSYQNFEAHSHTLKLKEHLNDSDYETSSE 417

BLAST of Spg002030 vs. ExPASy TrEMBL
Match: A0A6J1FAK0 (BRO1 domain-containing protein BROX homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442315 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.8e-208
Identity = 377/416 (90.62%), Postives = 396/416 (95.19%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMV+FVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVMFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELRR LEEYLSVLI LTK+ETIDDGLIEF+WRNLE+GRQEICVANFWF
Sbjct: 61  LVAEDTGGSAITELRRVLEEYLSVLICLTKKETIDDGLIEFKWRNLEHGRQEICVANFWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS++HLMAMLTLSEADSLMIPKD +GSGLRVVSSD KR+AIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVLHLMAMLTLSEADSLMIPKDPTGSGLRVVSSDCKREAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LV+IPFDIKKRLPK FQPG LEA+SIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQL+
Sbjct: 181 LVHIPFDIKKRLPKSFQPGALEAMSIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLV 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECDTDRGY KKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDTDRGYEKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELLLESK+ACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLLESKRACLSFCLASPVTRSPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVD-SAWSSENWEAQNQTLKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+D SAWSSE+ EAQ+QTLK+HLNDSDYET+SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSSAWSSEHCEAQSQTLKEHLNDSDYETLSE 416

BLAST of Spg002030 vs. ExPASy TrEMBL
Match: A0A5A7UZ61 (BRO1 domain-containing protein BROX-like protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00690 PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.3e-208
Identity = 378/417 (90.65%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIP+QSDLQRPLRGLI KDLADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPMQSDLQRPLRGLILKDLADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELR ALEEYLSVLIGLTK+ETIDDGLIEF+WRNLENGRQE C+AN WF
Sbjct: 61  LVAEDTGGSAITELRGALEEYLSVLIGLTKKETIDDGLIEFKWRNLENGRQETCIANLWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS+VHLMAMLTLSEADSLMIPKDHSGSG RVVSSDSKRDAIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVVHLMAMLTLSEADSLMIPKDHSGSGSRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LVYIPFDIKKRLP DF+PGVLEAISIQTLGQGTEIQLGLAVE+QNATLSVKRRLACEQLI
Sbjct: 181 LVYIPFDIKKRLPHDFKPGVLEAISIQTLGQGTEIQLGLAVETQNATLSVKRRLACEQLI 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECD DRGYGKKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDMDRGYGKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELL ESKKACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLGESKKACLSFCLASPVTRPPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQNQT--LKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+DSAWS +N+EA + T  LK+HLNDSDYET SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSAWSYQNFEAHSHTLKLKEHLNDSDYETSSE 417

BLAST of Spg002030 vs. ExPASy TrEMBL
Match: A0A1S3BNN0 (BRO1 domain-containing protein BROX homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491831 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.0e-208
Identity = 378/417 (90.65%), Postives = 391/417 (93.76%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIP+QSDLQRPLRGLI KDLADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPMQSDLQRPLRGLILKDLADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELR ALEEYLSVLIGLTK+ETIDDGLIEF+WRNLENGRQE C+AN WF
Sbjct: 61  LVAEDTGGSAITELRGALEEYLSVLIGLTKKETIDDGLIEFKWRNLENGRQETCIANLWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS+VHLMAMLTLSEADSLMIPKDHSGSG RVVSSDSKRDAIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVVHLMAMLTLSEADSLMIPKDHSGSGSRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LVYIPFDIKKRLP DF+PGVLEAISIQTLGQGTEIQLGLAVE+QNATLSVKRRLACEQLI
Sbjct: 181 LVYIPFDIKKRLPHDFKPGVLEAISIQTLGQGTEIQLGLAVETQNATLSVKRRLACEQLI 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECD DRGYGKKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDMDRGYGKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELL ESKKACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLGESKKACLSFCLASPVTRPPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQNQT--LKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+DSAWS +N+EA   T  LK+HLNDSDYET SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSAWSYQNFEAHTHTLKLKEHLNDSDYETSSE 417

BLAST of Spg002030 vs. ExPASy TrEMBL
Match: A0A0A0LBS0 (BRO1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G513980 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.0e-208
Identity = 377/417 (90.41%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIP+QSDLQRPLRGLI KD+ADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPMQSDLQRPLRGLILKDVADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELR ALEEYLSVLIGLTK+ETIDDGLIEF+WRNLENGRQE C+AN WF
Sbjct: 61  LVAEDTGGSAITELRGALEEYLSVLIGLTKKETIDDGLIEFKWRNLENGRQETCIANLWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS+VHLMAMLTLSEADSLMIPKDHSGSG RVVSSDSKRDAIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVVHLMAMLTLSEADSLMIPKDHSGSGSRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LVYIPFDIK+RLP DF+PGVLEAISIQTLGQGTEIQLGLAVE+QNATLSVKRRLACEQLI
Sbjct: 181 LVYIPFDIKRRLPNDFKPGVLEAISIQTLGQGTEIQLGLAVETQNATLSVKRRLACEQLI 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECD DRGYGKKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDMDRGYGKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELL ESKKACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLGESKKACLSFCLASPVTRPPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQNQT--LKQHLNDSDYETMSE 411
           ALQALPELPEFQLSLRPE+YQLPE+DSAWSS+N EA   T  LK+HLNDSDYET+SE
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSAWSSQNCEAYTHTLKLKEHLNDSDYETLSE 417

BLAST of Spg002030 vs. ExPASy TrEMBL
Match: A0A6J1J2A0 (BRO1 domain-containing protein BROX homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482058 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.5e-207
Identity = 375/413 (90.80%), Postives = 393/413 (95.16%), Query Frame = 0

Query: 1   MGCTSSVYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60
           MGCTSSVYAVGRKKKLSIPEMV+FVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV
Sbjct: 1   MGCTSSVYAVGRKKKLSIPEMVMFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQIV 60

Query: 61  LVAEDTGGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWRNLENGRQEICVANFWF 120
           LVAEDTGGSAITELRR LEEYLSVLI LTK+ETIDDGLIEF+WRNLE+GRQEICVANFWF
Sbjct: 61  LVAEDTGGSAITELRRVLEEYLSVLICLTKKETIDDGLIEFKWRNLEHGRQEICVANFWF 120

Query: 121 ELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAIDLLLKASGYLDCCIRDI 180
           ELLS++HLMAMLTLSEADSLMIPKD +GSGLRVVSSD KR+AIDLLLKASGYLDCCIRDI
Sbjct: 121 ELLSVLHLMAMLTLSEADSLMIPKDPTGSGLRVVSSDCKREAIDLLLKASGYLDCCIRDI 180

Query: 181 LVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLI 240
           LV+IPFDIKKRLPK FQPG LEA+SIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQL+
Sbjct: 181 LVHIPFDIKKRLPKSFQPGALEAMSIQTLGQGTEIQLGLAVESQNATLSVKRRLACEQLV 240

Query: 241 YFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEPSCHVSAVCC 300
           YFSQAYQCLSECDTDRGY KKHMSFIKWKFLE+KAAAYYYHGLILDKGSEPSCHVSAVCC
Sbjct: 241 YFSQAYQCLSECDTDRGYEKKHMSFIKWKFLESKAAAYYYHGLILDKGSEPSCHVSAVCC 300

Query: 301 FLAAEELLLESKKACLSFCLASPVTRL-----GCFHTSQKIPEVASRKSQMYGYLLEQEK 360
           FLAAEELLLESK+ACLSFCLASPVTR         H  QKIPEVASRKSQMYGYLLEQEK
Sbjct: 301 FLAAEELLLESKRACLSFCLASPVTRSPPLWGAMKHLHQKIPEVASRKSQMYGYLLEQEK 360

Query: 361 ALQALPELPEFQLSLRPEDYQLPEVD-SAWSSENWEAQNQTLKQHLNDSDYET 408
           ALQALPELPEFQLSLRPE+YQLPE+D S+WSSEN EAQ+QTLK+HLNDSDYET
Sbjct: 361 ALQALPELPEFQLSLRPEEYQLPEIDSSSWSSENCEAQSQTLKEHLNDSDYET 413

BLAST of Spg002030 vs. TAIR 10
Match: AT5G14020.1 (Endosomal targeting BRO1-like domain-containing protein )

HSP 1 Score: 486.1 bits (1250), Expect = 2.8e-137
Identity = 261/401 (65.09%), Postives = 310/401 (77.31%), Query Frame = 0

Query: 1   MGCTSSVYA-VGRK-KKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRNQ 60
           MGC  S  A VGRK KK  I E VVFV   R+PVQSDLQR L+G+  K   +RL+ LRNQ
Sbjct: 1   MGCAQSTIAVVGRKNKKRIIQESVVFVLQFRVPVQSDLQRQLKGVSPKTTINRLTCLRNQ 60

Query: 61  IVLVAEDTGGSAITELRRALEEYLSVLIGLTKRETID-DGLIEFRWRNLENGRQ-EICVA 120
           I LVAEDTGGSAI+ELR ALEEYLS+L GL K+     +G +E +WR L +GR+ EIC  
Sbjct: 61  IELVAEDTGGSAISELRTALEEYLSLLSGLIKKSKDGMEGCVELKWRTLGDGRRAEICCT 120

Query: 121 NFWFELLSIVHLMAMLTLSEADSLMIPKD----HSGSGLRVVSSDSKRDAIDLLLKASGY 180
           N W E+L ++H+MA L L+EA+SLMIPKD     SG+G+RVVS+D +RDA+DLLLKASGY
Sbjct: 121 NLWMEMLIVIHMMAALALTEANSLMIPKDCYASGSGNGVRVVSTDCRRDAVDLLLKASGY 180

Query: 181 LDCCIRDILVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQNATLSVKR 240
           L+ C+R+IL  +P DIK +LP D Q  VL+ +SIQ LGQGTEIQLGLAV+SQ ATLSVKR
Sbjct: 181 LEFCVREILTQLPHDIKSKLPDDMQESVLQTLSIQALGQGTEIQLGLAVDSQKATLSVKR 240

Query: 241 RLACEQLIYFSQAYQCLSECD-TDRGYGKKHMSFIKWKFLEAKAAAYYYHGLILDKGSEP 300
           R+ACEQ+IYF+QAY CLS C+    G  KK + FI WKFLEAKAAAYYYHGL+ DKGSEP
Sbjct: 241 RIACEQVIYFTQAYHCLSSCEAVSHGCAKKLLRFIYWKFLEAKAAAYYYHGLVTDKGSEP 300

Query: 301 SCHVSAVCCFLAAEELLLESKKACLSFCLASPVTRLGCF-----HTSQKIPEVASRKSQM 360
           +CH+SAVCCFLAA E+L ESKKACLSFCLA PVTR         H SQKIPEVA RKSQ 
Sbjct: 301 ACHLSAVCCFLAAAEILGESKKACLSFCLAPPVTRAPPMWGVMKHLSQKIPEVAFRKSQT 360

Query: 361 YGYLL-EQEKALQALPELPEFQLSLRPEDYQLPEVDSAWSS 387
           YGYLL E+EKA+Q LPELP+FQLSLRP+D++LPE++   SS
Sbjct: 361 YGYLLEEEEKAMQCLPELPDFQLSLRPDDFELPEIEDVSSS 401

BLAST of Spg002030 vs. TAIR 10
Match: AT1G73390.1 (Endosomal targeting BRO1-like domain-containing protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.9e-86
Identity = 169/414 (40.82%), Postives = 257/414 (62.08%), Query Frame = 0

Query: 1   MGCTSS---VYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRN 60
           MGC +S        R+K  SI ++ V+VP +RIP   +  + L   + K L +RL++LR 
Sbjct: 1   MGCFASRPNDTGGNRRKPTSIGDVSVYVPGLRIPKPVEFSQSLGDQLPKTLVERLTALRT 60

Query: 61  QIVLVAEDT-------------GGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWR 120
           +IV++A                GGS + +L  ALE+Y+ VL+GLTK  +     ++F W 
Sbjct: 61  RIVVMANQEGPTITRTRRKTQHGGSTLADLHHALEDYIPVLLGLTKDGSHLQFKVQFNWV 120

Query: 121 NLENGRQEICVANFWFELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAID 180
           N E+  +E  ++N W+E+LS++HLMAML +S+A+ L++P+  S      +S +++R +ID
Sbjct: 121 NQEDEEEETAMSNVWYEILSVLHLMAMLQMSQANLLLLPRGSSDGYHPKISEENRRASID 180

Query: 181 LLLKASGYLDCCIRDILVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQ 240
           + LKA+GYLDC ++ +L +   + ++ LP D   G L A+ +Q LGQG +IQLG+A++S 
Sbjct: 181 IFLKAAGYLDCAVKHVLPHFSTEQRRSLPIDLAEGALRALCLQALGQGVDIQLGMAIDSA 240

Query: 241 NATLSVKRRLACEQLIYFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLI 300
            ATL+VKRRL+CE + Y+ QA   L       G+G+KHM F+KWK++EAKAAAYYYHGLI
Sbjct: 241 KATLAVKRRLSCEMVKYWQQAQDNLMNLPLANGWGEKHMLFVKWKYVEAKAAAYYYHGLI 300

Query: 301 LDKGSEPSCHVSAVCCFLAAEELLLESKKACLSFCLASPVTRLGCF-----HTSQKIPEV 360
           LD+G+    H  AV    AA+E L ESKKA  +F  +SP +R         + S+KIP+ 
Sbjct: 301 LDEGNTEKSHGMAVAALQAADECLKESKKASEAFNTSSPTSRTPSLFGTMKYLSEKIPKE 360

Query: 361 ASRKSQMYGYLLEQEKALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQN 394
            S K ++   L   EK ++  P LP+F L+L+P++YQLP VD++WS  +   +N
Sbjct: 361 TSSKVRINRDLYSYEKIMETAPTLPDFALALKPDEYQLPSVDASWSEASLRTKN 414

BLAST of Spg002030 vs. TAIR 10
Match: AT1G73390.2 (Endosomal targeting BRO1-like domain-containing protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.9e-86
Identity = 169/414 (40.82%), Postives = 257/414 (62.08%), Query Frame = 0

Query: 1   MGCTSS---VYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRN 60
           MGC +S        R+K  SI ++ V+VP +RIP   +  + L   + K L +RL++LR 
Sbjct: 1   MGCFASRPNDTGGNRRKPTSIGDVSVYVPGLRIPKPVEFSQSLGDQLPKTLVERLTALRT 60

Query: 61  QIVLVAEDT-------------GGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWR 120
           +IV++A                GGS + +L  ALE+Y+ VL+GLTK  +     ++F W 
Sbjct: 61  RIVVMANQEGPTITRTRRKTQHGGSTLADLHHALEDYIPVLLGLTKDGSHLQFKVQFNWV 120

Query: 121 NLENGRQEICVANFWFELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAID 180
           N E+  +E  ++N W+E+LS++HLMAML +S+A+ L++P+  S      +S +++R +ID
Sbjct: 121 NQEDEEEETAMSNVWYEILSVLHLMAMLQMSQANLLLLPRGSSDGYHPKISEENRRASID 180

Query: 181 LLLKASGYLDCCIRDILVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQ 240
           + LKA+GYLDC ++ +L +   + ++ LP D   G L A+ +Q LGQG +IQLG+A++S 
Sbjct: 181 IFLKAAGYLDCAVKHVLPHFSTEQRRSLPIDLAEGALRALCLQALGQGVDIQLGMAIDSA 240

Query: 241 NATLSVKRRLACEQLIYFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLI 300
            ATL+VKRRL+CE + Y+ QA   L       G+G+KHM F+KWK++EAKAAAYYYHGLI
Sbjct: 241 KATLAVKRRLSCEMVKYWQQAQDNLMNLPLANGWGEKHMLFVKWKYVEAKAAAYYYHGLI 300

Query: 301 LDKGSEPSCHVSAVCCFLAAEELLLESKKACLSFCLASPVTRLGCF-----HTSQKIPEV 360
           LD+G+    H  AV    AA+E L ESKKA  +F  +SP +R         + S+KIP+ 
Sbjct: 301 LDEGNTEKSHGMAVAALQAADECLKESKKASEAFNTSSPTSRTPSLFGTMKYLSEKIPKE 360

Query: 361 ASRKSQMYGYLLEQEKALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQN 394
            S K ++   L   EK ++  P LP+F L+L+P++YQLP VD++WS  +   +N
Sbjct: 361 TSSKVRINRDLYSYEKIMETAPTLPDFALALKPDEYQLPSVDASWSEASLRTKN 414

BLAST of Spg002030 vs. TAIR 10
Match: AT1G73390.3 (Endosomal targeting BRO1-like domain-containing protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.9e-86
Identity = 169/414 (40.82%), Postives = 257/414 (62.08%), Query Frame = 0

Query: 1   MGCTSS---VYAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRN 60
           MGC +S        R+K  SI ++ V+VP +RIP   +  + L   + K L +RL++LR 
Sbjct: 1   MGCFASRPNDTGGNRRKPTSIGDVSVYVPGLRIPKPVEFSQSLGDQLPKTLVERLTALRT 60

Query: 61  QIVLVAEDT-------------GGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWR 120
           +IV++A                GGS + +L  ALE+Y+ VL+GLTK  +     ++F W 
Sbjct: 61  RIVVMANQEGPTITRTRRKTQHGGSTLADLHHALEDYIPVLLGLTKDGSHLQFKVQFNWV 120

Query: 121 NLENGRQEICVANFWFELLSIVHLMAMLTLSEADSLMIPKDHSGSGLRVVSSDSKRDAID 180
           N E+  +E  ++N W+E+LS++HLMAML +S+A+ L++P+  S      +S +++R +ID
Sbjct: 121 NQEDEEEETAMSNVWYEILSVLHLMAMLQMSQANLLLLPRGSSDGYHPKISEENRRASID 180

Query: 181 LLLKASGYLDCCIRDILVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVESQ 240
           + LKA+GYLDC ++ +L +   + ++ LP D   G L A+ +Q LGQG +IQLG+A++S 
Sbjct: 181 IFLKAAGYLDCAVKHVLPHFSTEQRRSLPIDLAEGALRALCLQALGQGVDIQLGMAIDSA 240

Query: 241 NATLSVKRRLACEQLIYFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGLI 300
            ATL+VKRRL+CE + Y+ QA   L       G+G+KHM F+KWK++EAKAAAYYYHGLI
Sbjct: 241 KATLAVKRRLSCEMVKYWQQAQDNLMNLPLANGWGEKHMLFVKWKYVEAKAAAYYYHGLI 300

Query: 301 LDKGSEPSCHVSAVCCFLAAEELLLESKKACLSFCLASPVTRLGCF-----HTSQKIPEV 360
           LD+G+    H  AV    AA+E L ESKKA  +F  +SP +R         + S+KIP+ 
Sbjct: 301 LDEGNTEKSHGMAVAALQAADECLKESKKASEAFNTSSPTSRTPSLFGTMKYLSEKIPKE 360

Query: 361 ASRKSQMYGYLLEQEKALQALPELPEFQLSLRPEDYQLPEVDSAWSSENWEAQN 394
            S K ++   L   EK ++  P LP+F L+L+P++YQLP VD++WS  +   +N
Sbjct: 361 TSSKVRINRDLYSYEKIMETAPTLPDFALALKPDEYQLPSVDASWSEASLRTKN 414

BLAST of Spg002030 vs. TAIR 10
Match: AT1G17940.1 (Endosomal targeting BRO1-like domain-containing protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.6e-79
Identity = 164/405 (40.49%), Postives = 250/405 (61.73%), Query Frame = 0

Query: 1   MGCTSSV---YAVGRKKKLSIPEMVVFVPSMRIPVQSDLQRPLRGLISKDLADRLSSLRN 60
           MGC +S        R+K  SI E+ V+VP +RIP   D  + L   + K L +RL++LR 
Sbjct: 1   MGCFASTPKDSGGNRRKPTSIGEVSVYVPGLRIPRAVDFLQSLGDQLPKTLVERLTALRT 60

Query: 61  QIVLVAEDT-------------GGSAITELRRALEEYLSVLIGLTKRETIDDGLIEFRWR 120
           +IV++A                GGS + +L +AL +YL V++GLTK  +     ++F W 
Sbjct: 61  RIVVMANQEGPTITRTRRKTQHGGSTLLDLHQALLDYLPVVLGLTKDGSHLQFKVQFNWV 120

Query: 121 NLENGRQEICVANFWFELLSIVHLMAMLTLSEADSLMIPKDHS-GSGLRVVSSDSKRDAI 180
           N E+  +E  ++N W+E+LS++HLMAML +S+A+ L++P+  S G     VS +++R +I
Sbjct: 121 NQEDEEEETEMSNVWYEVLSVLHLMAMLQMSQANLLLLPRGSSDGHNHPKVSEENRRTSI 180

Query: 181 DLLLKASGYLDCCIRDILVYIPFDIKKRLPKDFQPGVLEAISIQTLGQGTEIQLGLAVES 240
           D+ LKA+GYLDC ++ +L     + ++ LP D   G L A+ +Q LGQG +IQLG+A++S
Sbjct: 181 DIFLKAAGYLDCAVKHVLPQFSAEQRRSLPVDLAEGTLRALCLQALGQGVDIQLGMAIDS 240

Query: 241 QNATLSVKRRLACEQLIYFSQAYQCLSECDTDRGYGKKHMSFIKWKFLEAKAAAYYYHGL 300
             ATL+VKRRL+CE + Y+ QA   L       G+G+KH  F+KWK++EAKAAAYYYHGL
Sbjct: 241 SKATLAVKRRLSCEMVKYWQQAQDNLMNLPLANGWGEKHGLFVKWKYVEAKAAAYYYHGL 300

Query: 301 ILDKGSEPSCHVSAVCCFLAAEELLLESKKACLSFCLASPVTR----LGCF-HTSQKIPE 360
           ILD+G+    H  AV    AA+E   ESKKA  +F  ++P +R     G   + ++KIP+
Sbjct: 301 ILDEGNTEKSHGMAVAALQAADECFKESKKASEAFNASAPTSRTPPLFGTMKYLTEKIPK 360

Query: 361 VASRKSQMYGYLLEQEKALQALPELPEFQLSLRPEDYQLPEVDSA 384
             S K ++   L   EK ++  P LP+F L+L+P++YQLP  +++
Sbjct: 361 DTSSKVRINRDLYSYEKIMETAPTLPDFALALKPDEYQLPLAEAS 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877385.12.7e-21191.61BRO1 domain-containing protein BROX homolog isoform X1 [Benincasa hispida][more]
XP_022935442.13.7e-20890.63BRO1 domain-containing protein BROX homolog isoform X1 [Cucurbita moschata] >XP_... [more]
XP_038877386.14.8e-20890.89BRO1 domain-containing protein BROX homolog isoform X2 [Benincasa hispida][more]
XP_023527953.14.8e-20890.38BRO1 domain-containing protein BROX homolog isoform X1 [Cucurbita pepo subsp. pe... [more]
KAA0060334.14.8e-20890.65BRO1 domain-containing protein BROX-like protein isoform X1 [Cucumis melo var. m... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FAK01.8e-20890.63BRO1 domain-containing protein BROX homolog isoform X1 OS=Cucurbita moschata OX=... [more]
A0A5A7UZ612.3e-20890.65BRO1 domain-containing protein BROX-like protein isoform X1 OS=Cucumis melo var.... [more]
A0A1S3BNN03.0e-20890.65BRO1 domain-containing protein BROX homolog isoform X1 OS=Cucumis melo OX=3656 G... [more]
A0A0A0LBS03.0e-20890.41BRO1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G513980 PE=3 S... [more]
A0A6J1J2A01.5e-20790.80BRO1 domain-containing protein BROX homolog isoform X1 OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT5G14020.12.8e-13765.09Endosomal targeting BRO1-like domain-containing protein [more]
AT1G73390.14.9e-8640.82Endosomal targeting BRO1-like domain-containing protein [more]
AT1G73390.24.9e-8640.82Endosomal targeting BRO1-like domain-containing protein [more]
AT1G73390.34.9e-8640.82Endosomal targeting BRO1-like domain-containing protein [more]
AT1G17940.12.6e-7940.49Endosomal targeting BRO1-like domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004328BRO1 domainSMARTSM01041BRO1_2coord: 22..396
e-value: 3.0E-31
score: 119.8
IPR004328BRO1 domainPFAMPF03097BRO1coord: 49..311
e-value: 2.4E-11
score: 43.1
IPR004328BRO1 domainPROSITEPS51180BRO1coord: 6..410
score: 12.149065
IPR038499BRO1 domain superfamilyGENE3D1.25.40.280alix/aip1 like domainscoord: 41..393
e-value: 1.6E-38
score: 134.6
NoneNo IPR availablePANTHERPTHR23032:SF2ENDOSOMAL TARGETING BRO1-LIKE DOMAIN-CONTAINING PROTEINcoord: 1..407
NoneNo IPR availableCDDcd09034BRO1_Alix_likecoord: 14..373
e-value: 4.50211E-80
score: 248.418
IPR038898BRO1 domain-containing protein BROXPANTHERPTHR23032UNCHARACTERIZEDcoord: 1..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002030.1Spg002030.1mRNA