Spg002016 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002016
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncation/H(+) antiporter 15-like
Locationscaffold10: 1062876 .. 1065750 (-)
RNA-Seq ExpressionSpg002016
SyntenySpg002016
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCCCTTCTCCTCCATTTCCCTGAATCCTTCTTCCATTTCTTCATTTTTCGCAAATGGGTTCGATTGTAATGGAACCCGGCGACATCGCCGCCTTCAAGAACTCGACCTCCATCTGTACATTCGCCCGTCGAATCCACAGCGGCGGCGTTTTCGCCGGAGCCAACCCTCTGGAGTTCTCAGTTCCTCTTCTCTTCTTGCAGCTCGGAATCTGCGCTGGAACCATCATCTTGTTCTCTCGCCTTCTCAAACCCTTCGGCCAACCCCTCATCGTCTCGCAGATTCTGGTAATCTCTCCTCTTTTCTTCAACCCCTTTATTCCAAATTTCATTCTTCAGCTTCCTTATTCGTTCTAGTTGTGAATTTCATCAGGGCGCTTTGGTTCTTGGCTCTTCGGGTCTGAGCCACTTGGAGAAATTTAGAGAGGCGATTTTCCCTTTGAGGGGATTCATCATACTGGATGTGGTCTCTGCGCTTGGCCATGTCTTTTACTTCTTTCTGATTGGAGTGCAAACGGATATCTCCATTGTGAAGAGGATTGACAAGAAAGCATTTGGTATTGGGTTTTGCTCTGTGATTTCGCCTTCCATTCTCACTTTCATTTTCCCTTTCACCTTGATTAATATTGTTGACCCTCAAACTCTTCGATCCCTTGCAATTATTGGTAGAGCAGAGGTTTTCATCAACTTCACGATGGTTGCTTCCCTTCTTTCTGAGCTTCATTTGATAAACTCTAAGTTTGGGAGAATTGCCTTGTCATCTTCCATGGTTTCCAATTTCGTTAGCTTTTTTCTCCTGAAACTAGGAGCTCTCTTGACAACTGAGGCTAGATATAAGGTCTTGTCGACACTTTATTCTTTTGTAGTTATTGTAGTTGTCATCATTTTCGTTATTCGACCTGCCATTTTGTGGATGATAAAGCAGCATCCACTGGGACAGCCATTAAAGGAGGGCTTTGTGATTACATTGCTTTTAGTGGTGCTAGCAGCTGCTTTTTGTAGCAAGGCCTTTGGTTTGCATATCTATTTTGGTCCTCTTTTACTTGGGGTTACAATACCTTCAGGGCCTCCCCTTGGATCAGCATTGGTGGAGAGGCTTGATTTCATCACCTCCTGGCTTTTCATGCCCATCTTCTTTGTCAAAATAGGCTTGGTTGTCGATATCTATGCCATCGAACTCATAAATTTCTTATGCACCTCGTTCATTATCTTTCTCAGTGCATTGGGGAAGTTTTTGGGTGCCTTGTTGGTTTCAATCTACTTCAAACTACCTATGAGAGACGCCGTATCGCTCGGCCTCGTCTTGAATAGCCAAGGAGCTTTCGAGCTTGGTATGTATAAAATGATGAAGAAGGACAAGGTAAAAACAGGCAAATTTGTTACTGATCATCATTATCTAATTACATCTAGTCCTTTCTCACAAACTCACTGCCATTTTGTTTCCTAAATTCTTGCAGGCGATAAATGATGAAACATTTGTAGTTGCCTGCATATTCTTGTTGGTTCTGGCTGCAGTTATAGCTCCCATACTAAGATATCTCTTTGATCCTTCTCGGAGGTATGTAGTTTACAAGAGAAGAACTGTGATGCACTCTAGACCAGAGTTTGATCTGTGTGTATTAGTCTGCATTCATGACCAAGAAGATGTTCCAAGTGCCATTAACCTACTTGACGCCTTGAATCCGACGAGACGAAGCCATCTCGATGTGTACATGCTTCATCTTGTCGAGCTTCTCGGTCGTGCTACCCCGCAACTCATTCACCACAAGCTTACGAAAGTAAGGACTTCAAGGCATTGCCCTTCTGAGCATATTGTCAATGCCTTCAAATACTTTGGACAGCGCAACCGTGAAATTGTTAAAATTTATCCCTTCACTGCAATATCACCTCCTGCATCTATGCACGATGATGTTTGTTCCCTTGCACATGAAAAGAAGACTTCCTTGATTCTTGTTCCTTTCCACAAAAGATTTCATTCCAACGGCGTGATGTCGTTGTCCAAAAGCAAAATGAAAATGGTTAACCTTCATATCCTCGACAAGGCGCCCTGCTCGGTCGCCCTTGTCGTCGAGCGTGGAATTTTGAACGTCTCGAGGTCTATTGCTACCAATTTGCATCCCTTTCAGGTTGTTGTGGTCTTCATAGGTGGACCAGACGACCGTGAGGCAATGTTCATCGGAGCAAGGATGGTTGAACATCCCAATATCAACTTCACAATGATTCGACTGCTGGAGGACATGGATATCCCAAGCGATAACGTTAACGAGAGGATGCTCGATAATGAGGCTGTGTTCGAGTTTCGACAAATCATGGCCAACAACTATAGGGTGAGGTACATAGAAGAGGTGGTTAAAGACAGCACTGGAACGGTCTCGATACTTCGTTCGATGGGGAACAATTTCGATCTCGTAATGGTCGGAAGACGACATAGCCCGTGTTCTGTGATGGTTCAAGGCTTGGTATTTTGGGATGAGCAAACAGAACTTGGGATAATTGGGGAGGTATTGGCCTCTTCAGATTTCATGGGCAATACCATGATCTTGGTTGTGCAACAACACACAAATGTGGTAGATGAAGACCAATGGAATCATCAAGAAACTATCATTTCCATTGATAATACTTAGAAGATGATAATGCAAAAGAGTTGCCCATCCAAAGCAAATCATGAGAGTTTTGATTTTTCTCCTCCCCTTGACTAACCTTTTCTTTTTTAATTCAAAGTTGGTGAGGTTATTTTTGCTCCCCCTCTCTCTTTCTTCCTTTTTACTTTTAGCTTCTCAATAGTGTAAATTCCCCAATCAAAAACATTTCCATATGTTAACTAATTAAGTACATTATCTATATTTGGTCAATTTGACTTTTAGTGATGAGCTTTGAA

mRNA sequence

ATGGGTTCGATTGTAATGGAACCCGGCGACATCGCCGCCTTCAAGAACTCGACCTCCATCTGTACATTCGCCCGTCGAATCCACAGCGGCGGCGTTTTCGCCGGAGCCAACCCTCTGGAGTTCTCAGTTCCTCTTCTCTTCTTGCAGCTCGGAATCTGCGCTGGAACCATCATCTTGTTCTCTCGCCTTCTCAAACCCTTCGGCCAACCCCTCATCGTCTCGCAGATTCTGGGCGCTTTGGTTCTTGGCTCTTCGGGTCTGAGCCACTTGGAGAAATTTAGAGAGGCGATTTTCCCTTTGAGGGGATTCATCATACTGGATGTGGTCTCTGCGCTTGGCCATGTCTTTTACTTCTTTCTGATTGGAGTGCAAACGGATATCTCCATTGTGAAGAGGATTGACAAGAAAGCATTTGGTATTGGGTTTTGCTCTGTGATTTCGCCTTCCATTCTCACTTTCATTTTCCCTTTCACCTTGATTAATATTGTTGACCCTCAAACTCTTCGATCCCTTGCAATTATTGGTAGAGCAGAGGTTTTCATCAACTTCACGATGGTTGCTTCCCTTCTTTCTGAGCTTCATTTGATAAACTCTAAGTTTGGGAGAATTGCCTTGTCATCTTCCATGGTTTCCAATTTCGTTAGCTTTTTTCTCCTGAAACTAGGAGCTCTCTTGACAACTGAGGCTAGATATAAGGTCTTGTCGACACTTTATTCTTTTGTAGTTATTGTAGTTGTCATCATTTTCGTTATTCGACCTGCCATTTTGTGGATGATAAAGCAGCATCCACTGGGACAGCCATTAAAGGAGGGCTTTGTGATTACATTGCTTTTAGTGGTGCTAGCAGCTGCTTTTTGTAGCAAGGCCTTTGGTTTGCATATCTATTTTGGTCCTCTTTTACTTGGGGTTACAATACCTTCAGGGCCTCCCCTTGGATCAGCATTGGTGGAGAGGCTTGATTTCATCACCTCCTGGCTTTTCATGCCCATCTTCTTTGTCAAAATAGGCTTGGTTGTCGATATCTATGCCATCGAACTCATAAATTTCTTATGCACCTCGTTCATTATCTTTCTCAGTGCATTGGGGAAGTTTTTGGGTGCCTTGTTGGTTTCAATCTACTTCAAACTACCTATGAGAGACGCCGTATCGCTCGGCCTCGTCTTGAATAGCCAAGGAGCTTTCGAGCTTGGTATGTATAAAATGATGAAGAAGGACAAGGCGATAAATGATGAAACATTTGTAGTTGCCTGCATATTCTTGTTGGTTCTGGCTGCAGTTATAGCTCCCATACTAAGATATCTCTTTGATCCTTCTCGGAGGTATGTAGTTTACAAGAGAAGAACTGTGATGCACTCTAGACCAGAGTTTGATCTGTGTGTATTAGTCTGCATTCATGACCAAGAAGATGTTCCAAGTGCCATTAACCTACTTGACGCCTTGAATCCGACGAGACGAAGCCATCTCGATGTGTACATGCTTCATCTTGTCGAGCTTCTCGGTCGTGCTACCCCGCAACTCATTCACCACAAGCTTACGAAAGTAAGGACTTCAAGGCATTGCCCTTCTGAGCATATTGTCAATGCCTTCAAATACTTTGGACAGCGCAACCGTGAAATTGTTAAAATTTATCCCTTCACTGCAATATCACCTCCTGCATCTATGCACGATGATGTTTGTTCCCTTGCACATGAAAAGAAGACTTCCTTGATTCTTGTTCCTTTCCACAAAAGATTTCATTCCAACGGCGTGATGTCGTTGTCCAAAAGCAAAATGAAAATGGTTAACCTTCATATCCTCGACAAGGCGCCCTGCTCGGTCGCCCTTGTCGTCGAGCGTGGAATTTTGAACGTCTCGAGGTCTATTGCTACCAATTTGCATCCCTTTCAGGTTGTTGTGGTCTTCATAGGTGGACCAGACGACCGTGAGGCAATGTTCATCGGAGCAAGGATGGTTGAACATCCCAATATCAACTTCACAATGATTCGACTGCTGGAGGACATGGATATCCCAAGCGATAACGTTAACGAGAGGATGCTCGATAATGAGGCTGTGTTCGAGTTTCGACAAATCATGGCCAACAACTATAGGGTGAGGTACATAGAAGAGGTGGTTAAAGACAGCACTGGAACGGTCTCGATACTTCGTTCGATGGGGAACAATTTCGATCTCGTAATGGTCGGAAGACGACATAGCCCGTGTTCTGTGATGGTTCAAGGCTTGGTATTTTGGGATGAGCAAACAGAACTTGGGATAATTGGGGAGGTATTGGCCTCTTCAGATTTCATGGGCAATACCATGATCTTGGTTGTGCAACAACACACAAATGTGGTAGATGAAGACCAATGGAATCATCAAGAAACTATCATTTCCATTGATAATACTTAG

Coding sequence (CDS)

ATGGGTTCGATTGTAATGGAACCCGGCGACATCGCCGCCTTCAAGAACTCGACCTCCATCTGTACATTCGCCCGTCGAATCCACAGCGGCGGCGTTTTCGCCGGAGCCAACCCTCTGGAGTTCTCAGTTCCTCTTCTCTTCTTGCAGCTCGGAATCTGCGCTGGAACCATCATCTTGTTCTCTCGCCTTCTCAAACCCTTCGGCCAACCCCTCATCGTCTCGCAGATTCTGGGCGCTTTGGTTCTTGGCTCTTCGGGTCTGAGCCACTTGGAGAAATTTAGAGAGGCGATTTTCCCTTTGAGGGGATTCATCATACTGGATGTGGTCTCTGCGCTTGGCCATGTCTTTTACTTCTTTCTGATTGGAGTGCAAACGGATATCTCCATTGTGAAGAGGATTGACAAGAAAGCATTTGGTATTGGGTTTTGCTCTGTGATTTCGCCTTCCATTCTCACTTTCATTTTCCCTTTCACCTTGATTAATATTGTTGACCCTCAAACTCTTCGATCCCTTGCAATTATTGGTAGAGCAGAGGTTTTCATCAACTTCACGATGGTTGCTTCCCTTCTTTCTGAGCTTCATTTGATAAACTCTAAGTTTGGGAGAATTGCCTTGTCATCTTCCATGGTTTCCAATTTCGTTAGCTTTTTTCTCCTGAAACTAGGAGCTCTCTTGACAACTGAGGCTAGATATAAGGTCTTGTCGACACTTTATTCTTTTGTAGTTATTGTAGTTGTCATCATTTTCGTTATTCGACCTGCCATTTTGTGGATGATAAAGCAGCATCCACTGGGACAGCCATTAAAGGAGGGCTTTGTGATTACATTGCTTTTAGTGGTGCTAGCAGCTGCTTTTTGTAGCAAGGCCTTTGGTTTGCATATCTATTTTGGTCCTCTTTTACTTGGGGTTACAATACCTTCAGGGCCTCCCCTTGGATCAGCATTGGTGGAGAGGCTTGATTTCATCACCTCCTGGCTTTTCATGCCCATCTTCTTTGTCAAAATAGGCTTGGTTGTCGATATCTATGCCATCGAACTCATAAATTTCTTATGCACCTCGTTCATTATCTTTCTCAGTGCATTGGGGAAGTTTTTGGGTGCCTTGTTGGTTTCAATCTACTTCAAACTACCTATGAGAGACGCCGTATCGCTCGGCCTCGTCTTGAATAGCCAAGGAGCTTTCGAGCTTGGTATGTATAAAATGATGAAGAAGGACAAGGCGATAAATGATGAAACATTTGTAGTTGCCTGCATATTCTTGTTGGTTCTGGCTGCAGTTATAGCTCCCATACTAAGATATCTCTTTGATCCTTCTCGGAGGTATGTAGTTTACAAGAGAAGAACTGTGATGCACTCTAGACCAGAGTTTGATCTGTGTGTATTAGTCTGCATTCATGACCAAGAAGATGTTCCAAGTGCCATTAACCTACTTGACGCCTTGAATCCGACGAGACGAAGCCATCTCGATGTGTACATGCTTCATCTTGTCGAGCTTCTCGGTCGTGCTACCCCGCAACTCATTCACCACAAGCTTACGAAAGTAAGGACTTCAAGGCATTGCCCTTCTGAGCATATTGTCAATGCCTTCAAATACTTTGGACAGCGCAACCGTGAAATTGTTAAAATTTATCCCTTCACTGCAATATCACCTCCTGCATCTATGCACGATGATGTTTGTTCCCTTGCACATGAAAAGAAGACTTCCTTGATTCTTGTTCCTTTCCACAAAAGATTTCATTCCAACGGCGTGATGTCGTTGTCCAAAAGCAAAATGAAAATGGTTAACCTTCATATCCTCGACAAGGCGCCCTGCTCGGTCGCCCTTGTCGTCGAGCGTGGAATTTTGAACGTCTCGAGGTCTATTGCTACCAATTTGCATCCCTTTCAGGTTGTTGTGGTCTTCATAGGTGGACCAGACGACCGTGAGGCAATGTTCATCGGAGCAAGGATGGTTGAACATCCCAATATCAACTTCACAATGATTCGACTGCTGGAGGACATGGATATCCCAAGCGATAACGTTAACGAGAGGATGCTCGATAATGAGGCTGTGTTCGAGTTTCGACAAATCATGGCCAACAACTATAGGGTGAGGTACATAGAAGAGGTGGTTAAAGACAGCACTGGAACGGTCTCGATACTTCGTTCGATGGGGAACAATTTCGATCTCGTAATGGTCGGAAGACGACATAGCCCGTGTTCTGTGATGGTTCAAGGCTTGGTATTTTGGGATGAGCAAACAGAACTTGGGATAATTGGGGAGGTATTGGCCTCTTCAGATTTCATGGGCAATACCATGATCTTGGTTGTGCAACAACACACAAATGTGGTAGATGAAGACCAATGGAATCATCAAGAAACTATCATTTCCATTGATAATACTTAG

Protein sequence

MGSIVMEPGDIAAFKNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRSLAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVNAFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVVQQHTNVVDEDQWNHQETIISIDNT
Homology
BLAST of Spg002016 vs. NCBI nr
Match: XP_022983542.1 (cation/H(+) antiporter 15-like [Cucurbita maxima])

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 581/806 (72.08%), Postives = 679/806 (84.24%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T ICTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGCDSHNTLKNITRICTFADRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGHVF+FFLIGVQTDIS VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFFFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALLT   E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLTPQDE 240

Query: 241 ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGL V+IYAI+LIN
Sbjct: 301 ALGLHIYTGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLAVNIYAIKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F++ALGKFLGALL+S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVNALGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I+PI+R+L DPSRR++V+KRRTVMHSRPEFDLCVLVCIHDQE
Sbjct: 421 NDEALVVMCVWVMVVVAIISPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR C S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+  REI+ IYPFTAISP  +MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKREILAIYPFTAISPSTTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+V+VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIVMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDST 720
           RMV HPNIN T IRL E  ++PS +V ER LDNE+V EFRQ +A+N RV+YIEEVVKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEVVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMIL 780
           GT+SILRS+G +FDLV+VGR+H+PC  +VQGLV W+EQTELG IGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGGDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETIISIDNT 795
           VVQQH  V++ED+  +QE II +DNT
Sbjct: 781 VVQQHRRVINEDEELNQEDIIPMDNT 806

BLAST of Spg002016 vs. NCBI nr
Match: XP_023528659.1 (cation/H(+) antiporter 15-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 577/806 (71.59%), Postives = 675/806 (83.75%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T ICTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGHNTLKNLTKICTFANRIHSPGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTII FS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIIFFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGHVFYFFLIGVQTDIS VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTIKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALL    E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQNE 240

Query: 241 ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+G+PLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPIIVWMIKKNPIGKPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+ A+GKFLGALL+S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVGAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLCVLVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LIHHK  +VRTSR C S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLAVYILHLVELLGRAHLELIHHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP A+MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSATMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCS+ALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSIALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDST 720
           RMV HPNIN T IRL E  ++PS +V ER LDN++V EFRQ +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNKSVDEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMIL 780
           GT+SILRSMG++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSMGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETIISIDNT 795
           VVQQH  VV+ED+  +QE II +DNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Spg002016 vs. NCBI nr
Match: XP_022934362.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 578/806 (71.71%), Postives = 677/806 (84.00%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T ICTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGHVFYFFLIGVQTDIS VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+SA+GKFLGALL+S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR C S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDST 720
           RMV HPNIN T IRL E  ++PS +V ER LDNE+V EFRQ +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMIL 780
           GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETIISIDNT 795
           VVQQH  VV+ED+  +QE II +DNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Spg002016 vs. NCBI nr
Match: KAG6581108.1 (Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 575/806 (71.34%), Postives = 677/806 (84.00%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T ICTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGHVFYFFLIGVQTDIS VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E  INF MVASL+SELHLINS+FGR++LS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESLINFPMVASLVSELHLINSEFGRLSLSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+SA+GKFLGALL+S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFLVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVNA 540
           DVPSAINLLDALNPTRRSHL V++LHLVELLGRA  +LI+HK  +VRTSR C S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVHILHLVELLGRAHVELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDST 720
           RMV HPNIN T IRL E  ++PS +V ER LDNE+V EFRQ +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMIL 780
           GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETIISIDNT 795
           VVQQH  VV+ED+  +QE II +DNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Spg002016 vs. NCBI nr
Match: KAG7017840.1 (Cation/H(+) antiporter 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 575/818 (70.29%), Postives = 677/818 (82.76%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T ICTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGHVFYFFLIGVQTDIS VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E  INF MVASL+SELHLINS+FGR++LS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESLINFPMVASLVSELHLINSEFGRLSLSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDK-- 420
           FLC SFI+F+SA+GKFLGALL+S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K  
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKEL 420

Query: 421 ----------AINDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEF 480
                      INDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEF
Sbjct: 421 IASLFLESTQLINDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFLVHKRRTVMHSRPEF 480

Query: 481 DLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRT 540
           DLC LVCIHDQEDVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRT
Sbjct: 481 DLCALVCIHDQEDVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRT 540

Query: 541 SRHCPSEHIVNAFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHK 600
           SR C S  IVNAFKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+
Sbjct: 541 SRSCSSIPIVNAFKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQ 600

Query: 601 RFHSNGVMSLSKSKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIG 660
           RFHSNGV+SLS+ K KMVN HIL+ APCSVALVVERGIL   +SIA+N HPFQ+ +VFIG
Sbjct: 601 RFHSNGVLSLSQYKTKMVNHHILENAPCSVALVVERGILKTPKSIASNFHPFQIAMVFIG 660

Query: 661 GPDDREAMFIGARMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYR 720
           GPDDREAMFIGARMV HPNIN T IRL E  ++PS +V ER LDNE+V EFRQ +A+N R
Sbjct: 661 GPDDREAMFIGARMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNR 720

Query: 721 VRYIEEVVKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVL 780
           V+YIEE+VKD  GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGEVL
Sbjct: 721 VKYIEEMVKDGIGTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVL 780

Query: 781 ASSDFMGNTMILVVQQHTNVVDEDQWNHQETIISIDNT 795
           ASSDF+GN  ILVVQQH  VV+ED+  +QE II +DNT
Sbjct: 781 ASSDFIGNATILVVQQHRRVVNEDEELNQEDIIPMDNT 818

BLAST of Spg002016 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.3e-140
Identity = 296/805 (36.77%), Postives = 475/805 (59.01%), Query Frame = 0

Query: 16  NSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQ 75
           +++ IC     I + GV+ G NPL+FS+PL  LQL +       F  +LKPF QP ++S+
Sbjct: 10  DASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISE 69

Query: 76  ILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDK 135
           ILG +VLG S L    KF   IFP R  ++L+ ++ +G +++ FL+GV+ DI +V++  K
Sbjct: 70  ILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGK 129

Query: 136 KAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS-LAIIGRAEVFINFTMVASLLSELH 195
           +A  I    ++ P ++   F F++    D     + +  +G A     F ++A +L+EL 
Sbjct: 130 RALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELK 189

Query: 196 LINSKFGRIALSSSMVSNFVSFFLLKLGALL--TTEARYKVLSTLYSFVVIVVVIIFVIR 255
           LIN++ GRI++S+++V++  ++ LL L   L  + +  +  L  + S  V + V +FV+R
Sbjct: 190 LINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVR 249

Query: 256 PAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLG 315
           P I W+I++ P G+   E  +  +L  V+ + F + A G H  FG  + G+ IP+G PLG
Sbjct: 250 PGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLG 309

Query: 316 SALVERLDFITSWLFMPIFFVKIGLVVDIYAIE-LINFLCTSFIIFLSALGKFLGALLVS 375
             L+E+L+   S L +P+FF   GL  +I AI+    +L    +IFL+  GK +G ++V+
Sbjct: 310 LTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVA 369

Query: 376 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 435
            +  +P+R+ ++LGL+LN++G  E+ +  + K  K ++DETF    +  LV+  VI PI+
Sbjct: 370 FFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIV 429

Query: 436 RYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDVY 495
             L+ P ++ V YKRRT+  ++P+ +L VLVC+H   +VP+ INLL+A +PT+RS + +Y
Sbjct: 430 TILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIY 489

Query: 496 MLHLVELLGRATPQLIHHKLTK----VRTSRHCPSEHIVNAFKYFGQRNREIVKIYPFTA 555
           +LHLVEL GRA+  LI H   K            S+HI+NAF+ + +++   V + P TA
Sbjct: 490 VLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTA 549

Query: 556 ISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLHILDKAPCSVA 615
           ISP ++MH+DVCSLA +K+ S I++PFHK+   +G M  +    ++VN ++L+ +PCSV 
Sbjct: 550 ISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVG 609

Query: 616 LVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMIRLLEDM 675
           ++V+RG LN +  + +N    QV V+F GGPDDREA+    RM +HP I  T++R + D 
Sbjct: 610 ILVDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDE 669

Query: 676 D----------------IPS-DNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGT 735
           D                IP  D+  +R LD++ +  FR   A    + YIE++V +   T
Sbjct: 670 DEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEET 729

Query: 736 VSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVV 795
           V+ +RSM ++ DL +VGR     S +  GL  W E  ELG IG++LASSDF     +LVV
Sbjct: 730 VAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVV 789

BLAST of Spg002016 vs. ExPASy Swiss-Prot
Match: Q9LMJ1 (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 3.3e-115
Identity = 252/776 (32.47%), Postives = 438/776 (56.44%), Query Frame = 0

Query: 20  ICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S GVF G++PL++++PL+ LQ+ +   T  L  RLLKP  Q +I +Q+L  
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 80  LVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDKKAFG 139
           ++LG S       + +   P+ G I L  +S LG   + FL+G++ D SI+++   KA  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 140 IGFCSVISP---SILTFIFPFTLINIVDPQTLRSLAIIGRAEVFINFTMVASLLSELHLI 199
           IG  S   P     LT +F     N+  P  +  ++ +       +F +  ++L+EL+++
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNL-PPDVVHCISTVISLNAMTSFPVTTTVLAELNIL 206

Query: 200 NSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFV---VIVVVIIFVIRP 259
           NS  GR+A + S+V    S+ +  +  +   +     L++++SFV    +++VI FV RP
Sbjct: 207 NSDLGRLATNCSIVCEAFSWIVALVFRMFLRDG---TLASVWSFVWVTALILVIFFVCRP 266

Query: 260 AILWMIKQHPLGQPLKEGFV--ITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPL 319
           AI+W+ ++  +    K G +    +++V+L  +  S+  G+H  FG   LGV++P GPPL
Sbjct: 267 AIIWLTERRSISID-KAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPL 326

Query: 320 GSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSALGKFLGALLVS 379
           G+ L  +L+   + L +P F    GL  + + I   +      +I ++   KFLG    S
Sbjct: 327 GTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAAS 386

Query: 380 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 439
            Y  + + DA SL L++  QG  E+    M K +K +N E F +  I LL++  +   ++
Sbjct: 387 AYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLV 446

Query: 440 RYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDV 499
             L+DPS+RY    +RT++ +R       +L+C+++ E+VPS +NLL+A  P+R S + V
Sbjct: 447 VCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISV 506

Query: 500 YMLHLVELLGRATPQLI-HHKLTKVRTSRHCPSEHIVNAFKYFGQRNREIVKIYPFTAIS 559
           + LHLVEL GRA   L+ HH++ K+  +    S HIVN F+ F Q+N+  +    FTA +
Sbjct: 507 FTLHLVELKGRAHAVLVPHHQMNKLDPNT-VQSTHIVNGFQRFEQQNQGTLMAQHFTAAA 566

Query: 560 PPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLHILDKAPCSVALV 619
           P +S++DD+C+LA +KK +LI++PFHK++  +G +      ++ +NL++L+KAPCSV + 
Sbjct: 567 PFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIF 626

Query: 620 VERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMIRLLEDMDI 679
           ++RG     RS+  +     V V+FI G DD EA+    R+ EHP ++ TMI       +
Sbjct: 627 IDRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSL 686

Query: 680 PSD---NVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDLVMV 739
             +   +V   + ++  + +F+    +  ++ Y EE+V+D   T  ++ S+G++FDLV+V
Sbjct: 687 QQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVV 746

Query: 740 GRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVVQQHTNVVDEDQW 783
           GR H   S ++ GL  W E  ELG+IG++ ASSDF  + +++  Q+  ++  ++ +
Sbjct: 747 GRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDNSY 796

BLAST of Spg002016 vs. ExPASy Swiss-Prot
Match: O22920 (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 8.4e-111
Identity = 255/779 (32.73%), Postives = 432/779 (55.46%), Query Frame = 0

Query: 20  ICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S G+F  +NPL++++PLL LQ+ +   T  L  R+L+P  Q +I +Q+L  
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 80  LVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDKKAFG 139
           +VLG S L H   +     P  G II+  +S +G V + FL+G++ D SI+++   KA  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 140 IGFCSVISPSILTFIFPFTLINIV------------DPQTLRSLAIIGRAEVFINFTMVA 199
           IG  S        + FPF+L N+             D  +  S AI        +F +  
Sbjct: 149 IGTAS--------YAFPFSLGNLTIMFISKTMGLPSDVISCTSSAI--SLSSMTSFPVTT 208

Query: 200 SLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFVVIVVVI 259
           ++L+EL+++NS+ GR+A   SMV    S+F+     L T +     L  L   + +++VI
Sbjct: 209 TVLAELNILNSELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTSLYALSMIIGLLLVI 268

Query: 260 IFVIRPAILWMIKQHPLGQPLKEGF-VITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIP 319
            FV RP I+W+ ++       K+      +LL++  A+   +A G+H  FG   LGV++P
Sbjct: 269 YFVFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLP 328

Query: 320 SGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAI---ELINFLCTSFIIFLSALGK 379
            GPPLG+ L  +L+   S LF+P F    GL  + + I      + +    I+ ++   K
Sbjct: 329 DGPPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCK 388

Query: 380 FLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVL 439
           FLG    S Y +  + DA+ L  ++  QG  E+    + K  + ++ E F +  I +L +
Sbjct: 389 FLGTAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFV 448

Query: 440 AAVIAPILRYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNP 499
             +   ++ YL+DPS+RY    +RT++++R     L +L+ +++ E+VPS +NLL+A  P
Sbjct: 449 TGISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYP 508

Query: 500 TRRSHLDVYMLHLVELLGRATPQLI-HHKLTKVRTSRHCPSEHIVNAFKYFGQRNREIVK 559
           TR + +  + LHLVEL GRA   L  HH++ K+  +    S HIVNAF+ F Q+ +  + 
Sbjct: 509 TRFNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNT-AQSTHIVNAFQRFEQKYQGALM 568

Query: 560 IYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLHILDK 619
              FTA +P +S+++D+C+LA +KK +LI++PFHK++  +G +      ++ +NL++LD 
Sbjct: 569 AQHFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDA 628

Query: 620 APCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMI 679
           APCSVA+ ++RG     RS+        V ++FIGG DD EA+ +  RM E P++N TMI
Sbjct: 629 APCSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMI 688

Query: 680 RLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNF 739
                  +  ++ ++ M +   + +F+   AN  ++ Y+EE+V+D   T  ++ S+G+ +
Sbjct: 689 HFRHKSALQDEDYSD-MSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAY 748

Query: 740 DLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVVQQHTNVVDED 781
           D+V+VGR H   S ++ GL  W E  ELG+IG++L S DF  + +++  QQ  +++  D
Sbjct: 749 DMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGDDLLAMD 795

BLAST of Spg002016 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.6e-108
Identity = 252/752 (33.51%), Postives = 422/752 (56.12%), Query Frame = 0

Query: 64  LKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGF--IILDVVSALGHVFYFFLI 123
           L+  G     S +L  ++L  S L      R   F    +  I+  + +A  ++ ++FL+
Sbjct: 76  LRRLGMIRFTSHMLTGVLLSKSFLKENSAARR-FFSTEDYKEIVFSLTAACSYMMFWFLM 135

Query: 124 GVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIV----DPQTLRSL--AIIG 183
           GV+ D  +++   +KA  IG  SV+  +++  +  F  +  V       TL SL   +I 
Sbjct: 136 GVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIY 195

Query: 184 RAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFL--------------LKL 243
             +   +F +V +LL EL L NS+ GR+A+SS+++S+F +  L               +L
Sbjct: 196 SIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTRL 255

Query: 244 GALLTTE--ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLV 303
           G++   +  A  + L      V+ V + I+V RP + ++IKQ P G+P+K  ++ T++++
Sbjct: 256 GSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVM 315

Query: 304 VLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVV 363
           V  +A  +      I+ GP +LG+ +P GPPLGSA++++ +      F+P F       +
Sbjct: 316 VSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTEI 375

Query: 364 DIYAI-------ELINFLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQG 423
           DI A+        +I  + TSF++      KF+   + ++++ +PM D  +L L+++ +G
Sbjct: 376 DISALFGWEGLNGIILIMVTSFVV------KFIFTTVPALFYGMPMEDCFALSLIMSFKG 435

Query: 424 AFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHS 483
            FELG Y +  +  ++  ETF VAC+++ + +A+I PILRYL+DPSR Y  Y++R + H 
Sbjct: 436 IFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHL 495

Query: 484 RPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLT 543
           +P  +L +L CI+  +D+   INLL+A+ P+R S +  Y+LHL+EL+G+A P  I HKL 
Sbjct: 496 KPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQ 555

Query: 544 KVRTSRHCPSEHIVNAFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILV 603
             RT     S +++ +F+ F +     V +  +TA+S P +MH D+C LA    TSLIL+
Sbjct: 556 TRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILL 615

Query: 604 PFHKRFHSNGVMSLSKSKM-KMVNLHILDKAPCSVALVVER---GILNVSRSIAT----- 663
           PFH+ + ++G   +S + M + +N  +LD APCSV + V R   G  N+S    T     
Sbjct: 616 PFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTV 675

Query: 664 -NLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMIRLL-EDMDIPSDNVNERMLDN 723
            NL  + + ++F+GG DDREA+ +  RM   P IN T++RL+  D     + V ++MLD+
Sbjct: 676 PNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKMLDD 735

Query: 724 EAVFEFRQIMANN-YRVRYIEEVVKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGL 773
           E +   R + +N    + Y E+ ++D+  T S+LRSM ++FD+ +VGR +   SV  +GL
Sbjct: 736 ELL---RDVKSNTLVDIFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTEGL 795

BLAST of Spg002016 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.3e-103
Identity = 250/790 (31.65%), Postives = 427/790 (54.05%), Query Frame = 0

Query: 16  NSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQ 75
           NST  C    +  S GVF G NP++F++PL  LQ+ I      + + LL+P  QP ++++
Sbjct: 4   NSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAE 63

Query: 76  ILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDK 135
           ++G ++LG S L   + F +A+FP +   +L+ ++ LG +F+ FL G++ D   ++R  K
Sbjct: 64  VIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGK 123

Query: 136 KAFGIGFCSVISPSIL----TFIFPFTLINIVDPQTLRSLAIIGRAEVFINFTMVASLLS 195
           KA GI    +  P  L    +F+   T+   V+      L  +G A     F ++A +L+
Sbjct: 124 KALGIALAGITLPFALGIGSSFVLKATISKGVNSTAF--LVFMGVALSITAFPVLARILA 183

Query: 196 ELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFV---VIVVVII 255
           EL L+ ++ GR+A+S++ V++  ++ LL L A+  + +    L +L+ F+     V+   
Sbjct: 184 ELKLLTTEIGRLAMSAAAVNDVAAWILLAL-AIALSGSNTSPLVSLWVFLSGCAFVIGAS 243

Query: 256 FVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSG 315
           F+I P   W+ ++   G+P++E ++   L VVL   F + A G+H  FG  ++GV IP  
Sbjct: 244 FIIPPIFRWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKE 303

Query: 316 PPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSA-LGKFLGA 375
            P   ALVE+++ + S LF+P++FV  GL  ++  I+         ++  +A  GK LG 
Sbjct: 304 GPFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILGT 363

Query: 376 LLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVI 435
           L VS+ FK+PMR+A++LG ++N++G  EL +  + K  K +ND+TF +  +  L    + 
Sbjct: 364 LGVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFIT 423

Query: 436 APILRYLFDPSRRYV---VYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTR 495
            P++  ++ P+RR      YK R V        L +L C H    +PS INLL+A     
Sbjct: 424 TPVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIE 483

Query: 496 RSH-LDVYMLHLVELLGRATPQLIHHKLTK------VRTSRHCPSEHIVNAFKYFGQRNR 555
           +   L VY LHL EL  R++  L+ HK+ K       R   +  ++ +V AF+ F Q +R
Sbjct: 484 KGEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSR 543

Query: 556 EIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLH 615
             V + P TAIS  + +H+D+C+ A  KK +++++PFHK    +G +  ++   + VN  
Sbjct: 544 --VNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRR 603

Query: 616 ILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNIN 675
           +L +APCSV + V+RG+   S+  A ++  + VVV+F GGPDDREA+  G RM EHP I 
Sbjct: 604 VLLQAPCSVGIFVDRGLGGSSQVSAQDV-SYSVVVLFFGGPDDREALAYGLRMAEHPGIV 663

Query: 676 FTMIRL---------LEDMDIPSDN-----VNERMLDNEAVFEFRQIMANNYRVRYIEEV 735
            T+ R          + ++++ ++N     V     D E + E R+I + +  V+++E+ 
Sbjct: 664 LTVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQ 723

Query: 736 VKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMG 774
           ++++   V          +L +VGR   P   +   +    E  ELG +G +L S +   
Sbjct: 724 IENAAVDVRSAIEEVRRSNLFLVGR--MPGGEIALAIRENSECPELGPVGSLLISPESST 783

BLAST of Spg002016 vs. ExPASy TrEMBL
Match: A0A6J1IZN7 (cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111482115 PE=4 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 581/806 (72.08%), Postives = 679/806 (84.24%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T ICTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGCDSHNTLKNITRICTFADRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGHVF+FFLIGVQTDIS VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFFFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALLT   E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLTPQDE 240

Query: 241 ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGL V+IYAI+LIN
Sbjct: 301 ALGLHIYTGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLAVNIYAIKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F++ALGKFLGALL+S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVNALGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I+PI+R+L DPSRR++V+KRRTVMHSRPEFDLCVLVCIHDQE
Sbjct: 421 NDEALVVMCVWVMVVVAIISPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR C S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+  REI+ IYPFTAISP  +MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKREILAIYPFTAISPSTTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+V+VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIVMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDST 720
           RMV HPNIN T IRL E  ++PS +V ER LDNE+V EFRQ +A+N RV+YIEEVVKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEVVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMIL 780
           GT+SILRS+G +FDLV+VGR+H+PC  +VQGLV W+EQTELG IGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGGDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETIISIDNT 795
           VVQQH  V++ED+  +QE II +DNT
Sbjct: 781 VVQQHRRVINEDEELNQEDIIPMDNT 806

BLAST of Spg002016 vs. ExPASy TrEMBL
Match: A0A6J1F2I8 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111441553 PE=4 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 578/806 (71.71%), Postives = 677/806 (84.00%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAF----------KNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSI+MEP D+AA+          KN T ICTFA RIHS GVF+G NPLEFSVPLLFLQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           G+ AGTIILFS LLKP GQPLIVSQILG +VLGSSGL HL +FRE IFPLRGF++LDV+S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGHVFYFFLIGVQTDIS VK+IDKKAF IG CSVI   IL+ I+  TL+NIVD QT++ 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLT--TE 240
           L  IG  E FINF MVASL+SELHLINS+FGR ALS++M SNF S  L  LGALL   +E
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            +Y+ +STLY+ +++V VI F IRP I+WMIK++P+GQPLKE FV+TLLL+VL  AFC +
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELIN 360
           A GLHIY GPL LGVTIPSGPP+GSALV+RLDFITSW+FMPIFFVKIGLVV+IYA +LIN
Sbjct: 301 ASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAI 420
           FLC SFI+F+SA+GKFLGALL+S YFKL MRDAVSLGL+LN QGA ELG++K+M+K+K I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQE 480
           NDE  VV C++++V+ A+I PI+R+L DPSRR++V+KRRTVMHSRPEFDLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVNA 540
           DVPSAINLLDALNPTRRSHL VY+LHLVELLGRA  +LI+HK  +VRTSR C S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSK 600
           FKYFG+   EI+ IYPFTAISP ++MHD VCSLA +KKTSLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGA 660
            K KMVN HIL+ APCSVALVVERGIL   +SIATN HPFQ+ +VFIGGPDDREAMFIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDST 720
           RMV HPNIN T IRL E  ++PS +V ER LDNE+V EFRQ +A+N RV+YIEE+VKD  
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMIL 780
           GT+SILRS+G++FDLV+VGR+H+PC  +VQGLV W+EQTELG IGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTNVVDEDQWNHQETIISIDNT 795
           VVQQH  VV+ED+  +QE II +DNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Spg002016 vs. ExPASy TrEMBL
Match: A0A5D3DF05 (Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00110 PE=4 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 3.1e-302
Identity = 549/801 (68.54%), Postives = 640/801 (79.90%), Query Frame = 0

Query: 1   MGSIVMEPGDIA----------AFKNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSIVMEP D+           AFKN ++ICTFA RIH  GVF GANPLEFSV LL  QL
Sbjct: 1   MGSIVMEPEDVVAYMNGETGHNAFKNFSTICTFANRIHCSGVFNGANPLEFSVSLLLFQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           GIC+GTIILFS+LLKP G PLIVSQILG LVLGSSGLSHLEKF+E +FPLRGF+ LDVVS
Sbjct: 61  GICSGTIILFSQLLKPLGLPLIVSQILGGLVLGSSGLSHLEKFKEKVFPLRGFVCLDVVS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGH+FYFFLIG+QTDISI+K ID KAFGIG CS I   +L   +   L NIVD + ++ 
Sbjct: 121 ALGHIFYFFLIGLQTDISILKEIDIKAFGIGSCSTIFAMLLISTYSLFLSNIVDVKKIQH 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEAR 240
           +      E FI++ MV SLL ELHLINSKFGRI+LS+SM S+ +   L  L A+ +T   
Sbjct: 181 IFEFASLESFISYPMVVSLLYELHLINSKFGRISLSASMASSLLKICLTILAAIRSTNGE 240

Query: 241 YK--VLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            K  V + ++S VV++++II+VIRPA LWM K++P+GQPLKE FVITLLL VL  AFC +
Sbjct: 241 TKDLVSTKIFSIVVLILLIIYVIRPATLWMAKENPIGQPLKEYFVITLLLGVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVD-IYAIELI 360
            FGL IYFG  LLG  IPS PP+GS LV+RL+FITSW+FMPIFFV+IGLV+D IY I L+
Sbjct: 301 TFGLRIYFGSFLLGFIIPSDPPIGSTLVDRLEFITSWIFMPIFFVRIGLVIDNIYTITLV 360

Query: 361 NFLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKA 420
           N L  SFIIF+SALGKFLG+L++S+Y+K PMRDA+SLGL+LNSQGA EL M+K+MKKDK 
Sbjct: 361 NLLSMSFIIFISALGKFLGSLIISMYYKFPMRDAISLGLILNSQGALELSMFKIMKKDKL 420

Query: 421 INDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQ 480
           I+DE FVV CI ++V+ A+I PI+RYL  PS+RY+V+KRRTVMHSRPEFDLCVLVCIHDQ
Sbjct: 421 IDDEAFVVGCISIMVIVAIITPIIRYLLRPSKRYIVHKRRTVMHSRPEFDLCVLVCIHDQ 480

Query: 481 EDVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVN 540
           EDVPS INLLDALNPTRRSHL VYMLHLVELLGRA P+LIHHK   VR+ R   S+ IVN
Sbjct: 481 EDVPSVINLLDALNPTRRSHLIVYMLHLVELLGRAQPKLIHHKHKMVRSLRSSSSKPIVN 540

Query: 541 AFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLS 600
           AFKYF      IV I  FTAISP  +MHDDVCSLA +K TSLIL PFHKR+HSNG++S S
Sbjct: 541 AFKYFEDSKSNIVAINLFTAISPSTTMHDDVCSLALDKSTSLILAPFHKRYHSNGMVSFS 600

Query: 601 KSKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIG 660
           K K+K+VN HILDKAPCSV L+VERG+L VS+SI TNL  FQ+VV+FIGGPDDREAMFIG
Sbjct: 601 KHKLKIVNHHILDKAPCSVGLIVERGLLRVSKSIETNLQSFQIVVIFIGGPDDREAMFIG 660

Query: 661 ARMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDS 720
           ARMV H NIN TMIRLL++ ++P D+  E+ LD+EAV EFRQI+ANNYRVRY EEVVKD 
Sbjct: 661 ARMVGHVNINLTMIRLLDNENVPKDDFKEKRLDDEAVAEFRQIIANNYRVRYKEEVVKDG 720

Query: 721 TGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMI 780
           T T+SILRSMG+NFDL+MVGRRHSP    VQGLV WDE+TELG IGEVLASSDFMGN  I
Sbjct: 721 TETISILRSMGSNFDLIMVGRRHSPFLSPVQGLVLWDERTELGAIGEVLASSDFMGNATI 780

Query: 781 LVVQQHTNVVDEDQWNHQETI 789
           LVVQQHT V +ED  N  ETI
Sbjct: 781 LVVQQHTKVANEDHENPPETI 801

BLAST of Spg002016 vs. ExPASy TrEMBL
Match: A0A1S3BPK0 (cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103491816 PE=4 SV=1)

HSP 1 Score: 1042.3 bits (2694), Expect = 1.0e-300
Identity = 548/801 (68.41%), Postives = 637/801 (79.53%), Query Frame = 0

Query: 1   MGSIVMEPGDIA----------AFKNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQL 60
           MGSIVMEP D+           AFKN ++ICTFA RIH  GVF GANPLEFSV LL  QL
Sbjct: 1   MGSIVMEPEDVVAYMNGETGHNAFKNFSTICTFANRIHCSGVFNGANPLEFSVSLLLFQL 60

Query: 61  GICAGTIILFSRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVS 120
           GIC+GTIILFS+LLKP G PLIVSQILG LVLGSSGLSHLEKF+E +FPLRGF+ LDVVS
Sbjct: 61  GICSGTIILFSQLLKPLGLPLIVSQILGGLVLGSSGLSHLEKFKEKVFPLRGFVCLDVVS 120

Query: 121 ALGHVFYFFLIGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS 180
           ALGH+FYFFLIG+QTDISI+K ID KAFGIG CS I   +L   +   L NIVD + ++ 
Sbjct: 121 ALGHIFYFFLIGLQTDISILKEIDIKAFGIGSCSTIFAMLLISTYSLFLSNIVDVKKIQH 180

Query: 181 LAIIGRAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEAR 240
           +      E FI++ MV SLL ELHLINSKFGRI+LS+SM S+ +   L  L A+ +T   
Sbjct: 181 IFEFASLESFISYPMVVSLLYELHLINSKFGRISLSASMASSLLKICLTILAAIRSTNGE 240

Query: 241 YK--VLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSK 300
            K  V +  +S VV++++II+VIRPA LWM K++P+GQPLKE FVITLLL VL  AFC +
Sbjct: 241 TKDLVSTKNFSIVVLILLIIYVIRPATLWMAKENPIGQPLKEYFVITLLLGVLVVAFCCQ 300

Query: 301 AFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVD-IYAIELI 360
            FGL IYFG  LLG  IPS PP+GS LV+RL+FITSW+FMPIFFV+IGLV+D IY I L+
Sbjct: 301 TFGLRIYFGSFLLGFIIPSDPPIGSTLVDRLEFITSWIFMPIFFVRIGLVIDNIYTITLV 360

Query: 361 NFLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKA 420
           N L  SFIIF+SALGKFLG+L++S+Y+K PMRDA+SLGL+LNSQGA EL M+K+MKKDK 
Sbjct: 361 NLLSMSFIIFISALGKFLGSLIISMYYKFPMRDAISLGLILNSQGALELSMFKIMKKDKL 420

Query: 421 INDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQ 480
           I+DE FVV CI ++V+ A+I PI+RYL  PS+RY+V+KRRTVMHSRPEFDLCVLVCIHDQ
Sbjct: 421 IDDEAFVVGCISIMVIVAIITPIIRYLLRPSKRYIVHKRRTVMHSRPEFDLCVLVCIHDQ 480

Query: 481 EDVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRTSRHCPSEHIVN 540
           EDVPS INLLDALNPTRRSHL VYMLHLVELLGRA P+LIHHK   VR+ R   SE IVN
Sbjct: 481 EDVPSVINLLDALNPTRRSHLIVYMLHLVELLGRAQPKLIHHKHKMVRSLRSSSSEPIVN 540

Query: 541 AFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLS 600
           AFKYF      IV I  FTAISP  +MHDDVCSLA +K TSLIL PFHKR+HSNG++S S
Sbjct: 541 AFKYFEDSKSNIVAINLFTAISPSTTMHDDVCSLALDKSTSLILAPFHKRYHSNGMVSFS 600

Query: 601 KSKMKMVNLHILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIG 660
           K K+K+VN HILDKAPCSV L+VERG+L VS+SI TNL  FQ+VV+FIGG DDRE MFIG
Sbjct: 601 KHKLKIVNHHILDKAPCSVGLIVERGLLRVSKSIETNLQSFQIVVIFIGGADDRETMFIG 660

Query: 661 ARMVEHPNINFTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDS 720
           ARMV H NIN TMIRLL++ ++P D+  E+ LD+EAV EFRQI+ANNYRVRY EEVVKD 
Sbjct: 661 ARMVGHVNINLTMIRLLDNENVPKDDFKEKRLDDEAVAEFRQIIANNYRVRYKEEVVKDG 720

Query: 721 TGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMI 780
           T T+SILRSMG+NFDL+MVGRRHSP    VQGLV WDE+TELG IGEVLASSDFMGN  I
Sbjct: 721 TETISILRSMGSNFDLIMVGRRHSPFLSPVQGLVLWDERTELGAIGEVLASSDFMGNATI 780

Query: 781 LVVQQHTNVVDEDQWNHQETI 789
           LVVQQHT V +ED  N  ETI
Sbjct: 781 LVVQQHTRVANEDHENPPETI 801

BLAST of Spg002016 vs. ExPASy TrEMBL
Match: A0A6J1EN94 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111435106 PE=4 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 1.3e-300
Identity = 529/783 (67.56%), Postives = 638/783 (81.48%), Query Frame = 0

Query: 1   MGSIVMEPGDIAAFKNSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILF 60
           MGSIVMEPGD + F N +SIC    RIHS  VF G NPLEFSVPLL LQLGIC+GTII  
Sbjct: 1   MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 61  SRLLKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFL 120
            +LLK   QPLIVSQIL  LVLG  G   LEKFRE IFP+RG I LD +SALG VFYFFL
Sbjct: 61  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 121 IGVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRSLAIIGRAEVF 180
           IGVQTD+SI+K IDK+AFGIG CSVI P+IL+ ++  TL+N VD  T+++L ++G AE F
Sbjct: 121 IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 181 INFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTE--ARYKVLSTLY 240
           I+F MVASLL+ELHLINSKFG+IAL+SSM SN    F  K+G LL  E   R   LST+Y
Sbjct: 181 IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 241 SFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGP 300
            F+V+ V+I+   +P ILWM K  P+GQPLKE FVITL L VL  AFCS+A G+HIYFGP
Sbjct: 241 GFIVLAVIIL-SFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGP 300

Query: 301 LLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFL 360
           +LLG+TIP GPP+GSA+VE+LD I SW+ MPIFF KIGL+  I+ I+L+NFLC SFII +
Sbjct: 301 ILLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIV 360

Query: 361 SALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACI 420
           +A GKFLGAL++SIY+KLP RD V+LGL+LNSQGA E+G++K+MKKDK +++ETFVV CI
Sbjct: 361 AAFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMKKDKVLDEETFVVMCI 420

Query: 421 FLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLD 480
            ++++AAVI PILRYLFDPSRR+V  K+RTVMH RPEFDL VLVCIHDQ+DVPSAIN+L+
Sbjct: 421 CVMLVAAVITPILRYLFDPSRRFVTEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILE 480

Query: 481 ALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLTKVRT-SRHCPSEHIVNAFKYFGQRNR 540
           AL PT RSHL VYMLHLV LLGRA PQLIHH++TK+RT SR   SE IVNAF YFGQ N 
Sbjct: 481 ALKPTHRSHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYVSESIVNAFNYFGQSNS 540

Query: 541 EIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLH 600
           ++V I+PFTA+SP  +M++DVCSLA +K+TSLIL+PFHKRFHSNG+MSLSK KMKM+N H
Sbjct: 541 DVVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQH 600

Query: 601 ILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNIN 660
           ILDKAPCSVAL+VERGI+ +SRSI+TNL+ FQ+ +VFIGGPDDREA+FIG RM+ HP IN
Sbjct: 601 ILDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYIN 660

Query: 661 FTMIRLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGTVSILRSM 720
            T+IRLLED + PSD   E  L+NEAV ++ ++M NN+RVRY+EEV KD TGTV+ILR++
Sbjct: 661 VTLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRTL 720

Query: 721 GNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVVQQHTNVV 780
            NNFDL++VGRRHSP S +VQGL+ W+EQTELG IGEVLASSDF+GN  I VVQQH  VV
Sbjct: 721 DNNFDLILVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVV 780

BLAST of Spg002016 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 501.9 bits (1291), Expect = 9.4e-142
Identity = 296/805 (36.77%), Postives = 475/805 (59.01%), Query Frame = 0

Query: 16  NSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQ 75
           +++ IC     I + GV+ G NPL+FS+PL  LQL +       F  +LKPF QP ++S+
Sbjct: 10  DASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISE 69

Query: 76  ILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDK 135
           ILG +VLG S L    KF   IFP R  ++L+ ++ +G +++ FL+GV+ DI +V++  K
Sbjct: 70  ILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGK 129

Query: 136 KAFGIGFCSVISPSILTFIFPFTLINIVDPQTLRS-LAIIGRAEVFINFTMVASLLSELH 195
           +A  I    ++ P ++   F F++    D     + +  +G A     F ++A +L+EL 
Sbjct: 130 RALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELK 189

Query: 196 LINSKFGRIALSSSMVSNFVSFFLLKLGALL--TTEARYKVLSTLYSFVVIVVVIIFVIR 255
           LIN++ GRI++S+++V++  ++ LL L   L  + +  +  L  + S  V + V +FV+R
Sbjct: 190 LINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVR 249

Query: 256 PAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLG 315
           P I W+I++ P G+   E  +  +L  V+ + F + A G H  FG  + G+ IP+G PLG
Sbjct: 250 PGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLG 309

Query: 316 SALVERLDFITSWLFMPIFFVKIGLVVDIYAIE-LINFLCTSFIIFLSALGKFLGALLVS 375
             L+E+L+   S L +P+FF   GL  +I AI+    +L    +IFL+  GK +G ++V+
Sbjct: 310 LTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVA 369

Query: 376 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 435
            +  +P+R+ ++LGL+LN++G  E+ +  + K  K ++DETF    +  LV+  VI PI+
Sbjct: 370 FFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIV 429

Query: 436 RYLFDPSRRYVVYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDVY 495
             L+ P ++ V YKRRT+  ++P+ +L VLVC+H   +VP+ INLL+A +PT+RS + +Y
Sbjct: 430 TILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIY 489

Query: 496 MLHLVELLGRATPQLIHHKLTK----VRTSRHCPSEHIVNAFKYFGQRNREIVKIYPFTA 555
           +LHLVEL GRA+  LI H   K            S+HI+NAF+ + +++   V + P TA
Sbjct: 490 VLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENY-EQHAAFVAVQPLTA 549

Query: 556 ISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLHILDKAPCSVA 615
           ISP ++MH+DVCSLA +K+ S I++PFHK+   +G M  +    ++VN ++L+ +PCSV 
Sbjct: 550 ISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVG 609

Query: 616 LVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMIRLLEDM 675
           ++V+RG LN +  + +N    QV V+F GGPDDREA+    RM +HP I  T++R + D 
Sbjct: 610 ILVDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDE 669

Query: 676 D----------------IPS-DNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGT 735
           D                IP  D+  +R LD++ +  FR   A    + YIE++V +   T
Sbjct: 670 DEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEET 729

Query: 736 VSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVV 795
           V+ +RSM ++ DL +VGR     S +  GL  W E  ELG IG++LASSDF     +LVV
Sbjct: 730 VAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVV 789

BLAST of Spg002016 vs. TAIR 10
Match: AT1G06970.1 (cation/hydrogen exchanger 14 )

HSP 1 Score: 417.5 bits (1072), Expect = 2.3e-116
Identity = 252/776 (32.47%), Postives = 438/776 (56.44%), Query Frame = 0

Query: 20  ICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S GVF G++PL++++PL+ LQ+ +   T  L  RLLKP  Q +I +Q+L  
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 80  LVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDKKAFG 139
           ++LG S       + +   P+ G I L  +S LG   + FL+G++ D SI+++   KA  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 140 IGFCSVISP---SILTFIFPFTLINIVDPQTLRSLAIIGRAEVFINFTMVASLLSELHLI 199
           IG  S   P     LT +F     N+  P  +  ++ +       +F +  ++L+EL+++
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNL-PPDVVHCISTVISLNAMTSFPVTTTVLAELNIL 206

Query: 200 NSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFV---VIVVVIIFVIRP 259
           NS  GR+A + S+V    S+ +  +  +   +     L++++SFV    +++VI FV RP
Sbjct: 207 NSDLGRLATNCSIVCEAFSWIVALVFRMFLRDG---TLASVWSFVWVTALILVIFFVCRP 266

Query: 260 AILWMIKQHPLGQPLKEGFV--ITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPL 319
           AI+W+ ++  +    K G +    +++V+L  +  S+  G+H  FG   LGV++P GPPL
Sbjct: 267 AIIWLTERRSISID-KAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPL 326

Query: 320 GSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSALGKFLGALLVS 379
           G+ L  +L+   + L +P F    GL  + + I   +      +I ++   KFLG    S
Sbjct: 327 GTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAAS 386

Query: 380 IYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPIL 439
            Y  + + DA SL L++  QG  E+    M K +K +N E F +  I LL++  +   ++
Sbjct: 387 AYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLV 446

Query: 440 RYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDV 499
             L+DPS+RY    +RT++ +R       +L+C+++ E+VPS +NLL+A  P+R S + V
Sbjct: 447 VCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISV 506

Query: 500 YMLHLVELLGRATPQLI-HHKLTKVRTSRHCPSEHIVNAFKYFGQRNREIVKIYPFTAIS 559
           + LHLVEL GRA   L+ HH++ K+  +    S HIVN F+ F Q+N+  +    FTA +
Sbjct: 507 FTLHLVELKGRAHAVLVPHHQMNKLDPNT-VQSTHIVNGFQRFEQQNQGTLMAQHFTAAA 566

Query: 560 PPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLHILDKAPCSVALV 619
           P +S++DD+C+LA +KK +LI++PFHK++  +G +      ++ +NL++L+KAPCSV + 
Sbjct: 567 PFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIF 626

Query: 620 VERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMIRLLEDMDI 679
           ++RG     RS+  +     V V+FI G DD EA+    R+ EHP ++ TMI       +
Sbjct: 627 IDRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSL 686

Query: 680 PSD---NVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNFDLVMV 739
             +   +V   + ++  + +F+    +  ++ Y EE+V+D   T  ++ S+G++FDLV+V
Sbjct: 687 QQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVV 746

Query: 740 GRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVVQQHTNVVDEDQW 783
           GR H   S ++ GL  W E  ELG+IG++ ASSDF  + +++  Q+  ++  ++ +
Sbjct: 747 GRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDNSY 796

BLAST of Spg002016 vs. TAIR 10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 402.9 bits (1034), Expect = 5.9e-112
Identity = 255/779 (32.73%), Postives = 432/779 (55.46%), Query Frame = 0

Query: 20  ICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQILGA 79
           +C     + S G+F  +NPL++++PLL LQ+ +   T  L  R+L+P  Q +I +Q+L  
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 80  LVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDKKAFG 139
           +VLG S L H   +     P  G II+  +S +G V + FL+G++ D SI+++   KA  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 140 IGFCSVISPSILTFIFPFTLINIV------------DPQTLRSLAIIGRAEVFINFTMVA 199
           IG  S        + FPF+L N+             D  +  S AI        +F +  
Sbjct: 149 IGTAS--------YAFPFSLGNLTIMFISKTMGLPSDVISCTSSAI--SLSSMTSFPVTT 208

Query: 200 SLLSELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFVVIVVVI 259
           ++L+EL+++NS+ GR+A   SMV    S+F+     L T +     L  L   + +++VI
Sbjct: 209 TVLAELNILNSELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTSLYALSMIIGLLLVI 268

Query: 260 IFVIRPAILWMIKQHPLGQPLKEGF-VITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIP 319
            FV RP I+W+ ++       K+      +LL++  A+   +A G+H  FG   LGV++P
Sbjct: 269 YFVFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLP 328

Query: 320 SGPPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAI---ELINFLCTSFIIFLSALGK 379
            GPPLG+ L  +L+   S LF+P F    GL  + + I      + +    I+ ++   K
Sbjct: 329 DGPPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCK 388

Query: 380 FLGALLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVL 439
           FLG    S Y +  + DA+ L  ++  QG  E+    + K  + ++ E F +  I +L +
Sbjct: 389 FLGTAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFV 448

Query: 440 AAVIAPILRYLFDPSRRYVVYKRRTVMHSRP-EFDLCVLVCIHDQEDVPSAINLLDALNP 499
             +   ++ YL+DPS+RY    +RT++++R     L +L+ +++ E+VPS +NLL+A  P
Sbjct: 449 TGISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYP 508

Query: 500 TRRSHLDVYMLHLVELLGRATPQLI-HHKLTKVRTSRHCPSEHIVNAFKYFGQRNREIVK 559
           TR + +  + LHLVEL GRA   L  HH++ K+  +    S HIVNAF+ F Q+ +  + 
Sbjct: 509 TRFNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNT-AQSTHIVNAFQRFEQKYQGALM 568

Query: 560 IYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLHILDK 619
              FTA +P +S+++D+C+LA +KK +LI++PFHK++  +G +      ++ +NL++LD 
Sbjct: 569 AQHFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDA 628

Query: 620 APCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMI 679
           APCSVA+ ++RG     RS+        V ++FIGG DD EA+ +  RM E P++N TMI
Sbjct: 629 APCSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMI 688

Query: 680 RLLEDMDIPSDNVNERMLDNEAVFEFRQIMANNYRVRYIEEVVKDSTGTVSILRSMGNNF 739
                  +  ++ ++ M +   + +F+   AN  ++ Y+EE+V+D   T  ++ S+G+ +
Sbjct: 689 HFRHKSALQDEDYSD-MSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAY 748

Query: 740 DLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMGNTMILVVQQHTNVVDED 781
           D+V+VGR H   S ++ GL  W E  ELG+IG++L S DF  + +++  QQ  +++  D
Sbjct: 749 DMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGDDLLAMD 795

BLAST of Spg002016 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 393.3 bits (1009), Expect = 4.7e-109
Identity = 252/752 (33.51%), Postives = 422/752 (56.12%), Query Frame = 0

Query: 64  LKPFGQPLIVSQILGALVLGSSGLSHLEKFREAIFPLRGF--IILDVVSALGHVFYFFLI 123
           L+  G     S +L  ++L  S L      R   F    +  I+  + +A  ++ ++FL+
Sbjct: 76  LRRLGMIRFTSHMLTGVLLSKSFLKENSAARR-FFSTEDYKEIVFSLTAACSYMMFWFLM 135

Query: 124 GVQTDISIVKRIDKKAFGIGFCSVISPSILTFIFPFTLINIV----DPQTLRSL--AIIG 183
           GV+ D  +++   +KA  IG  SV+  +++  +  F  +  V       TL SL   +I 
Sbjct: 136 GVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIY 195

Query: 184 RAEVFINFTMVASLLSELHLINSKFGRIALSSSMVSNFVSFFL--------------LKL 243
             +   +F +V +LL EL L NS+ GR+A+SS+++S+F +  L               +L
Sbjct: 196 SIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTRL 255

Query: 244 GALLTTE--ARYKVLSTLYSFVVIVVVIIFVIRPAILWMIKQHPLGQPLKEGFVITLLLV 303
           G++   +  A  + L      V+ V + I+V RP + ++IKQ P G+P+K  ++ T++++
Sbjct: 256 GSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVM 315

Query: 304 VLAAAFCSKAFGLHIYFGPLLLGVTIPSGPPLGSALVERLDFITSWLFMPIFFVKIGLVV 363
           V  +A  +      I+ GP +LG+ +P GPPLGSA++++ +      F+P F       +
Sbjct: 316 VSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTEI 375

Query: 364 DIYAI-------ELINFLCTSFIIFLSALGKFLGALLVSIYFKLPMRDAVSLGLVLNSQG 423
           DI A+        +I  + TSF++      KF+   + ++++ +PM D  +L L+++ +G
Sbjct: 376 DISALFGWEGLNGIILIMVTSFVV------KFIFTTVPALFYGMPMEDCFALSLIMSFKG 435

Query: 424 AFELGMYKMMKKDKAINDETFVVACIFLLVLAAVIAPILRYLFDPSRRYVVYKRRTVMHS 483
            FELG Y +  +  ++  ETF VAC+++ + +A+I PILRYL+DPSR Y  Y++R + H 
Sbjct: 436 IFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHL 495

Query: 484 RPEFDLCVLVCIHDQEDVPSAINLLDALNPTRRSHLDVYMLHLVELLGRATPQLIHHKLT 543
           +P  +L +L CI+  +D+   INLL+A+ P+R S +  Y+LHL+EL+G+A P  I HKL 
Sbjct: 496 KPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQ 555

Query: 544 KVRTSRHCPSEHIVNAFKYFGQRNREIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILV 603
             RT     S +++ +F+ F +     V +  +TA+S P +MH D+C LA    TSLIL+
Sbjct: 556 TRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILL 615

Query: 604 PFHKRFHSNGVMSLSKSKM-KMVNLHILDKAPCSVALVVER---GILNVSRSIAT----- 663
           PFH+ + ++G   +S + M + +N  +LD APCSV + V R   G  N+S    T     
Sbjct: 616 PFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTV 675

Query: 664 -NLHPFQVVVVFIGGPDDREAMFIGARMVEHPNINFTMIRLL-EDMDIPSDNVNERMLDN 723
            NL  + + ++F+GG DDREA+ +  RM   P IN T++RL+  D     + V ++MLD+
Sbjct: 676 PNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKMLDD 735

Query: 724 EAVFEFRQIMANN-YRVRYIEEVVKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGL 773
           E +   R + +N    + Y E+ ++D+  T S+LRSM ++FD+ +VGR +   SV  +GL
Sbjct: 736 ELL---RDVKSNTLVDIFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTEGL 795

BLAST of Spg002016 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 379.0 bits (972), Expect = 9.2e-105
Identity = 250/790 (31.65%), Postives = 427/790 (54.05%), Query Frame = 0

Query: 16  NSTSICTFARRIHSGGVFAGANPLEFSVPLLFLQLGICAGTIILFSRLLKPFGQPLIVSQ 75
           NST  C    +  S GVF G NP++F++PL  LQ+ I      + + LL+P  QP ++++
Sbjct: 4   NSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAE 63

Query: 76  ILGALVLGSSGLSHLEKFREAIFPLRGFIILDVVSALGHVFYFFLIGVQTDISIVKRIDK 135
           ++G ++LG S L   + F +A+FP +   +L+ ++ LG +F+ FL G++ D   ++R  K
Sbjct: 64  VIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGK 123

Query: 136 KAFGIGFCSVISPSIL----TFIFPFTLINIVDPQTLRSLAIIGRAEVFINFTMVASLLS 195
           KA GI    +  P  L    +F+   T+   V+      L  +G A     F ++A +L+
Sbjct: 124 KALGIALAGITLPFALGIGSSFVLKATISKGVNSTAF--LVFMGVALSITAFPVLARILA 183

Query: 196 ELHLINSKFGRIALSSSMVSNFVSFFLLKLGALLTTEARYKVLSTLYSFV---VIVVVII 255
           EL L+ ++ GR+A+S++ V++  ++ LL L A+  + +    L +L+ F+     V+   
Sbjct: 184 ELKLLTTEIGRLAMSAAAVNDVAAWILLAL-AIALSGSNTSPLVSLWVFLSGCAFVIGAS 243

Query: 256 FVIRPAILWMIKQHPLGQPLKEGFVITLLLVVLAAAFCSKAFGLHIYFGPLLLGVTIPSG 315
           F+I P   W+ ++   G+P++E ++   L VVL   F + A G+H  FG  ++GV IP  
Sbjct: 244 FIIPPIFRWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKE 303

Query: 316 PPLGSALVERLDFITSWLFMPIFFVKIGLVVDIYAIELINFLCTSFIIFLSA-LGKFLGA 375
            P   ALVE+++ + S LF+P++FV  GL  ++  I+         ++  +A  GK LG 
Sbjct: 304 GPFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILGT 363

Query: 376 LLVSIYFKLPMRDAVSLGLVLNSQGAFELGMYKMMKKDKAINDETFVVACIFLLVLAAVI 435
           L VS+ FK+PMR+A++LG ++N++G  EL +  + K  K +ND+TF +  +  L    + 
Sbjct: 364 LGVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFIT 423

Query: 436 APILRYLFDPSRRYV---VYKRRTVMHSRPEFDLCVLVCIHDQEDVPSAINLLDALNPTR 495
            P++  ++ P+RR      YK R V        L +L C H    +PS INLL+A     
Sbjct: 424 TPVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIE 483

Query: 496 RSH-LDVYMLHLVELLGRATPQLIHHKLTK------VRTSRHCPSEHIVNAFKYFGQRNR 555
           +   L VY LHL EL  R++  L+ HK+ K       R   +  ++ +V AF+ F Q +R
Sbjct: 484 KGEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSR 543

Query: 556 EIVKIYPFTAISPPASMHDDVCSLAHEKKTSLILVPFHKRFHSNGVMSLSKSKMKMVNLH 615
             V + P TAIS  + +H+D+C+ A  KK +++++PFHK    +G +  ++   + VN  
Sbjct: 544 --VNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRR 603

Query: 616 ILDKAPCSVALVVERGILNVSRSIATNLHPFQVVVVFIGGPDDREAMFIGARMVEHPNIN 675
           +L +APCSV + V+RG+   S+  A ++  + VVV+F GGPDDREA+  G RM EHP I 
Sbjct: 604 VLLQAPCSVGIFVDRGLGGSSQVSAQDV-SYSVVVLFFGGPDDREALAYGLRMAEHPGIV 663

Query: 676 FTMIRL---------LEDMDIPSDN-----VNERMLDNEAVFEFRQIMANNYRVRYIEEV 735
            T+ R          + ++++ ++N     V     D E + E R+I + +  V+++E+ 
Sbjct: 664 LTVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQ 723

Query: 736 VKDSTGTVSILRSMGNNFDLVMVGRRHSPCSVMVQGLVFWDEQTELGIIGEVLASSDFMG 774
           ++++   V          +L +VGR   P   +   +    E  ELG +G +L S +   
Sbjct: 724 IENAAVDVRSAIEEVRRSNLFLVGR--MPGGEIALAIRENSECPELGPVGSLLISPESST 783

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022983542.10.0e+0072.08cation/H(+) antiporter 15-like [Cucurbita maxima][more]
XP_023528659.10.0e+0071.59cation/H(+) antiporter 15-like [Cucurbita pepo subsp. pepo][more]
XP_022934362.10.0e+0071.71cation/H(+) antiporter 15-like [Cucurbita moschata][more]
KAG6581108.10.0e+0071.34Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7017840.10.0e+0070.29Cation/H(+) antiporter 15 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9SIT51.3e-14036.77Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9LMJ13.3e-11532.47Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1[more]
O229208.4e-11132.73Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1[more]
Q9FFB86.6e-10833.51Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9FFR91.3e-10331.65Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IZN70.0e+0072.08cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111482115 PE=4 ... [more]
A0A6J1F2I80.0e+0071.71cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111441553 PE=... [more]
A0A5D3DF053.1e-30268.54Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BPK01.0e-30068.41cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103491816 PE=4 SV=1[more]
A0A6J1EN941.3e-30067.56cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111435106 PE=... [more]
Match NameE-valueIdentityDescription
AT2G13620.19.4e-14236.77cation/hydrogen exchanger 15 [more]
AT1G06970.12.3e-11632.47cation/hydrogen exchanger 14 [more]
AT2G30240.15.9e-11232.73Cation/hydrogen exchanger family protein [more]
AT5G22900.14.7e-10933.51cation/H+ exchanger 3 [more]
AT5G41610.19.2e-10531.65cation/H+ exchanger 18 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 56..431
e-value: 1.2E-30
score: 106.6
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 45..441
e-value: 5.4E-51
score: 175.6
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 11..782
NoneNo IPR availablePANTHERPTHR32468:SF114CATION/H(+) ANTIPORTER 15-LIKE ISOFORM X1coord: 11..782

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002016.1Spg002016.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity