Spg001869 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg001869 vs. NCBI nr
Match: XP_016899697.1 (PREDICTED: uncharacterized protein LOC107990618 [Cucumis melo] >KAA0053688.1 uncharacterized protein E6C27_scaffold135G00610 [Cucumis melo var. makuwa] >TYK13985.1 uncharacterized protein E5676_scaffold1119G00100 [Cucumis melo var. makuwa])

HSP 1 Score: 481.9 bits (1239), Expect = 4.7e-132
Identity = 264/352 (75.00%), Postives = 298/352 (84.66%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           MESHQILSKPKLGFSASF+EA KIL NHPKFISLIIFFSFPLLASLLAH  LLHPTFI L
Sbjct: 1   MESHQILSKPKLGFSASFKEALKILFNHPKFISLIIFFSFPLLASLLAHQILLHPTFIHL 60

Query: 61  LKLLDHEHPFNLYNIV-AISCINT-----NGTARNCRPSVNGSFKATLSRKFLISTLLFS 120
           LKLL H  PF   +IV  I C N+          N  P +  +FK  LS++FLISTLL +
Sbjct: 61  LKLLFHHDPFQPISIVRIIDCRNSTLCVLEPLDDNPSPPIK-TFKEILSQRFLISTLLLT 120

Query: 121 ALVFFLDLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLAS 180
           +++FFLDLLNTIATVSISAA+YGGN+QMGFKEMLV+V KMVAL+L+G++ TSLC +LLAS
Sbjct: 121 SIIFFLDLLNTIATVSISAAIYGGNSQMGFKEMLVQVIKMVALKLKGAIETSLCFILLAS 180

Query: 181 LTLLGFVALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNM 240
           +TLLG VAL TDF F++K   FM +DI ++SK +I T  FGSLFVVLL KYIEWSAVWNM
Sbjct: 181 VTLLGLVALSTDFFFITKDHHFMMLDIIFVSKFTIVTLFFGSLFVVLLGKYIEWSAVWNM 240

Query: 241 GIIVSILDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGV 300
           GI++SILDKN+GYIAIGVASYLSRGSR+LGFSLMMVFFVLKV FGLPCLY+LWNEGSCGV
Sbjct: 241 GIVISILDKNKGYIAIGVASYLSRGSRRLGFSLMMVFFVLKVAFGLPCLYSLWNEGSCGV 300

Query: 301 LGNVLFISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENN-GKAIKAVQQ 346
           LGNV+F+ LNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENN  KA +AVQQ
Sbjct: 301 LGNVVFVILNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENNEKKAFEAVQQ 351

BLAST of Spg001869 vs. NCBI nr
Match: KGN59645.1 (hypothetical protein Csa_002408 [Cucumis sativus])

HSP 1 Score: 481.1 bits (1237), Expect = 8.0e-132
Identity = 261/355 (73.52%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           ME+HQILSKPKLGFSASF+EA KIL NHPKFISLIIFFSFPLLASLLAH  LLHPTFI+L
Sbjct: 1   MENHQILSKPKLGFSASFKEALKILFNHPKFISLIIFFSFPLLASLLAHQILLHPTFIRL 60

Query: 61  LKLLDHEHPFNLYNIVAISCINTNGTARNC---------RPSVNGSFKATLSRKFLISTL 120
           LKLL H  PF+  +IV +  IN       C          P +  +FK TLS++FLISTL
Sbjct: 61  LKLLYHHDPFHPTSIVRLIKINCKSPNVYCALQPLDDNPSPPIK-TFKETLSQRFLISTL 120

Query: 121 LFSALVFFLDLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVL 180
           L ++++FFLDLLNTIATVSISAA+YGGN+QMGFKEMLV++RKMVAL+L+G++ TSLC +L
Sbjct: 121 LLTSIIFFLDLLNTIATVSISAAIYGGNSQMGFKEMLVQIRKMVALKLKGAIETSLCFIL 180

Query: 181 LASLTLLGFVALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAV 240
           LASLTLLG VAL  DF +++K   FM  DI ++SK +IFT  FGSLFVVLL KYIEWSAV
Sbjct: 181 LASLTLLGLVALSKDFFYITKDHPFMMSDIIFVSKFTIFTLFFGSLFVVLLGKYIEWSAV 240

Query: 241 WNMGIIVSILDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGS 300
           WNMGI++SILDKN+GYIAIGVASYLSRGSR+LGFSLMMVF VLKV FGLPCLYALWNEG+
Sbjct: 241 WNMGIVISILDKNKGYIAIGVASYLSRGSRRLGFSLMMVFLVLKVAFGLPCLYALWNEGN 300

Query: 301 CGVLGNVLFISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLEN-NGKAIKAVQQ 346
           CGVLGNV+F+ LNCVGNVVMWVVLMVYFYDCKREFLEKKVDLEN   KA +AVQQ
Sbjct: 301 CGVLGNVVFVILNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENTEKKAFEAVQQ 354

BLAST of Spg001869 vs. NCBI nr
Match: XP_022935545.1 (uncharacterized protein LOC111442386 [Cucurbita moschata])

HSP 1 Score: 471.5 bits (1212), Expect = 6.4e-129
Identity = 259/345 (75.07%), Postives = 289/345 (83.77%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           ME+HQILSKPKLGFSASFREA+KIL NHP FISLIIF S PLLASL AH+ LLHPTFIQL
Sbjct: 1   MENHQILSKPKLGFSASFREAFKILFNHPNFISLIIFLSLPLLASLSAHNILLHPTFIQL 60

Query: 61  LKLLDHEHPFNLYNIVAISCINTNGTARNCRPSVNGSFKATLSRKFLISTLLFSALVFFL 120
           L+ L H++P++L     I C   +GTA            +TLS  FLIS L+ SAL+FFL
Sbjct: 61  LRFLSHKNPYDLSLTSIIVCPILDGTA------CFSQLSSTLSHGFLISILVSSALIFFL 120

Query: 121 DLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLASLTLLGF 180
           DLLNTIA VSISAALYGGN+QMGFKEMLV+VRKMVAL+LRGSLATSLCSVLLASLTLLG 
Sbjct: 121 DLLNTIAIVSISAALYGGNSQMGFKEMLVQVRKMVALKLRGSLATSLCSVLLASLTLLGL 180

Query: 181 VALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNMGIIVSI 240
           VAL  DF  M+ G S MSI   ++ ++ IFT IFG+ FVVLL KY+EWSAVWNMGI++SI
Sbjct: 181 VALSKDFFLMATGSSHMSITNTFLLQLFIFTFIFGTPFVVLLGKYVEWSAVWNMGIVISI 240

Query: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGVLGNVLF 300
           LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKV+FG PCLYALWNE SCGV  NV+F
Sbjct: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVVFGFPCLYALWNERSCGVWENVMF 300

Query: 301 ISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENNGKAIKAVQQ 346
           +SLN VGNVVMWVVLMVYFYDCKRE LEKK+DLENNGK+I+AVQQ
Sbjct: 301 VSLNGVGNVVMWVVLMVYFYDCKRELLEKKIDLENNGKSIEAVQQ 339

BLAST of Spg001869 vs. NCBI nr
Match: XP_038904922.1 (uncharacterized protein LOC120091132 [Benincasa hispida])

HSP 1 Score: 471.5 bits (1212), Expect = 6.4e-129
Identity = 265/351 (75.50%), Postives = 297/351 (84.62%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           MESHQILSKPKLGFS SFREA KIL NHPKF+SLIIFFSFPLLASLLA+  LLHPTFI L
Sbjct: 1   MESHQILSKPKLGFSTSFREALKILFNHPKFVSLIIFFSFPLLASLLANQILLHPTFIHL 60

Query: 61  LKLLDHEHPFNLYNIVAISCINTNGTARNC----RPSVNGSFKATLSRKFLISTLLFSAL 120
           LKLL H    N  +I+ I  IN   +AR C     P    SFK T S+ FLISTLL   +
Sbjct: 61  LKLLYHHSLHNPPSILRI--INCKASARICISEAIPFPIKSFKETHSQSFLISTLLLPVI 120

Query: 121 VFFLDLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLASLT 180
           +FFLDLLNTIA VSISAA+YGGN+QMGFKEMLV+VRKMVAL+LRGS+ TSL SVLLASLT
Sbjct: 121 IFFLDLLNTIAIVSISAAIYGGNSQMGFKEMLVQVRKMVALKLRGSVETSLFSVLLASLT 180

Query: 181 LLGFVALPTDFLFMSKGISFMS-IDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNMG 240
           LLG VAL TD  + +KGISFMS IDI +I++++IFT  FGS+FVVLL KYIEWSAVW+MG
Sbjct: 181 LLGLVALSTDIFYRTKGISFMSIIDISFITELTIFTLFFGSIFVVLLGKYIEWSAVWHMG 240

Query: 241 IIVSILDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGVL 300
           I++SILDKN+GYIAIGVASYLSRGSRKLG SLMMVFFVLK++FG PCLYALWNEGSCGVL
Sbjct: 241 IVISILDKNKGYIAIGVASYLSRGSRKLGLSLMMVFFVLKMVFGFPCLYALWNEGSCGVL 300

Query: 301 GNVLFISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENN-GKAIKAVQQ 346
           GNV+F+SLNC+ NVVMWVVLMVYFYDCKREFL KKVDLENN  KAI+AV+Q
Sbjct: 301 GNVVFVSLNCMENVVMWVVLMVYFYDCKREFLGKKVDLENNEKKAIEAVKQ 349

BLAST of Spg001869 vs. NCBI nr
Match: KAG6581012.1 (hypothetical protein SDJN03_21014, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 462.6 bits (1189), Expect = 2.9e-126
Identity = 257/345 (74.49%), Postives = 286/345 (82.90%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           ME+HQILSKPKLGFSASFREA+KIL NHP FISLIIF S PLLASL AH+ LLHPTFIQL
Sbjct: 1   MENHQILSKPKLGFSASFREAFKILFNHPNFISLIIFISLPLLASLSAHNILLHPTFIQL 60

Query: 61  LKLLDHEHPFNLYNIVAISCINTNGTARNCRPSVNGSFKATLSRKFLISTLLFSALVFFL 120
           L+ L H++P++      I C   +GTA            +TLS  FLIS L+ SAL+FFL
Sbjct: 61  LRFLSHKNPYDPSLTSIIVCQILDGTA------CFSQLSSTLSHGFLISILVSSALIFFL 120

Query: 121 DLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLASLTLLGF 180
           DLLNTIA VSISAALYGGN+QMGFKEMLV+VRKMVALRL GSLATSL SVLLASLTLLG 
Sbjct: 121 DLLNTIAIVSISAALYGGNSQMGFKEMLVQVRKMVALRLIGSLATSLSSVLLASLTLLGL 180

Query: 181 VALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNMGIIVSI 240
           VAL  DF FM+ G S +SI   ++ ++ IFT IFG+ FVVLL KY+EWSAVWNMGI++SI
Sbjct: 181 VALSKDFFFMATGSSHISITNTFLLQLFIFTFIFGTPFVVLLGKYVEWSAVWNMGIVISI 240

Query: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGVLGNVLF 300
           LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKV+FG PCLYALWNE SCGV  NV+F
Sbjct: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVVFGFPCLYALWNERSCGVWENVVF 300

Query: 301 ISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENNGKAIKAVQQ 346
           +SLN VGNVVMWVVLMVYFYDCKRE LEKK+DLENNGK I+AVQQ
Sbjct: 301 VSLNGVGNVVMWVVLMVYFYDCKRELLEKKIDLENNGKPIEAVQQ 339

BLAST of Spg001869 vs. ExPASy TrEMBL
Match: A0A5D3CQZ0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119G00100 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.3e-132
Identity = 264/352 (75.00%), Postives = 298/352 (84.66%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           MESHQILSKPKLGFSASF+EA KIL NHPKFISLIIFFSFPLLASLLAH  LLHPTFI L
Sbjct: 1   MESHQILSKPKLGFSASFKEALKILFNHPKFISLIIFFSFPLLASLLAHQILLHPTFIHL 60

Query: 61  LKLLDHEHPFNLYNIV-AISCINT-----NGTARNCRPSVNGSFKATLSRKFLISTLLFS 120
           LKLL H  PF   +IV  I C N+          N  P +  +FK  LS++FLISTLL +
Sbjct: 61  LKLLFHHDPFQPISIVRIIDCRNSTLCVLEPLDDNPSPPIK-TFKEILSQRFLISTLLLT 120

Query: 121 ALVFFLDLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLAS 180
           +++FFLDLLNTIATVSISAA+YGGN+QMGFKEMLV+V KMVAL+L+G++ TSLC +LLAS
Sbjct: 121 SIIFFLDLLNTIATVSISAAIYGGNSQMGFKEMLVQVIKMVALKLKGAIETSLCFILLAS 180

Query: 181 LTLLGFVALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNM 240
           +TLLG VAL TDF F++K   FM +DI ++SK +I T  FGSLFVVLL KYIEWSAVWNM
Sbjct: 181 VTLLGLVALSTDFFFITKDHHFMMLDIIFVSKFTIVTLFFGSLFVVLLGKYIEWSAVWNM 240

Query: 241 GIIVSILDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGV 300
           GI++SILDKN+GYIAIGVASYLSRGSR+LGFSLMMVFFVLKV FGLPCLY+LWNEGSCGV
Sbjct: 241 GIVISILDKNKGYIAIGVASYLSRGSRRLGFSLMMVFFVLKVAFGLPCLYSLWNEGSCGV 300

Query: 301 LGNVLFISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENN-GKAIKAVQQ 346
           LGNV+F+ LNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENN  KA +AVQQ
Sbjct: 301 LGNVVFVILNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENNEKKAFEAVQQ 351

BLAST of Spg001869 vs. ExPASy TrEMBL
Match: A0A1S4DUN1 (uncharacterized protein LOC107990618 OS=Cucumis melo OX=3656 GN=LOC107990618 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.3e-132
Identity = 264/352 (75.00%), Postives = 298/352 (84.66%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           MESHQILSKPKLGFSASF+EA KIL NHPKFISLIIFFSFPLLASLLAH  LLHPTFI L
Sbjct: 1   MESHQILSKPKLGFSASFKEALKILFNHPKFISLIIFFSFPLLASLLAHQILLHPTFIHL 60

Query: 61  LKLLDHEHPFNLYNIV-AISCINT-----NGTARNCRPSVNGSFKATLSRKFLISTLLFS 120
           LKLL H  PF   +IV  I C N+          N  P +  +FK  LS++FLISTLL +
Sbjct: 61  LKLLFHHDPFQPISIVRIIDCRNSTLCVLEPLDDNPSPPIK-TFKEILSQRFLISTLLLT 120

Query: 121 ALVFFLDLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLAS 180
           +++FFLDLLNTIATVSISAA+YGGN+QMGFKEMLV+V KMVAL+L+G++ TSLC +LLAS
Sbjct: 121 SIIFFLDLLNTIATVSISAAIYGGNSQMGFKEMLVQVIKMVALKLKGAIETSLCFILLAS 180

Query: 181 LTLLGFVALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNM 240
           +TLLG VAL TDF F++K   FM +DI ++SK +I T  FGSLFVVLL KYIEWSAVWNM
Sbjct: 181 VTLLGLVALSTDFFFITKDHHFMMLDIIFVSKFTIVTLFFGSLFVVLLGKYIEWSAVWNM 240

Query: 241 GIIVSILDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGV 300
           GI++SILDKN+GYIAIGVASYLSRGSR+LGFSLMMVFFVLKV FGLPCLY+LWNEGSCGV
Sbjct: 241 GIVISILDKNKGYIAIGVASYLSRGSRRLGFSLMMVFFVLKVAFGLPCLYSLWNEGSCGV 300

Query: 301 LGNVLFISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENN-GKAIKAVQQ 346
           LGNV+F+ LNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENN  KA +AVQQ
Sbjct: 301 LGNVVFVILNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENNEKKAFEAVQQ 351

BLAST of Spg001869 vs. ExPASy TrEMBL
Match: A0A0A0LCA3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G835820 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.9e-132
Identity = 261/355 (73.52%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           ME+HQILSKPKLGFSASF+EA KIL NHPKFISLIIFFSFPLLASLLAH  LLHPTFI+L
Sbjct: 1   MENHQILSKPKLGFSASFKEALKILFNHPKFISLIIFFSFPLLASLLAHQILLHPTFIRL 60

Query: 61  LKLLDHEHPFNLYNIVAISCINTNGTARNC---------RPSVNGSFKATLSRKFLISTL 120
           LKLL H  PF+  +IV +  IN       C          P +  +FK TLS++FLISTL
Sbjct: 61  LKLLYHHDPFHPTSIVRLIKINCKSPNVYCALQPLDDNPSPPIK-TFKETLSQRFLISTL 120

Query: 121 LFSALVFFLDLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVL 180
           L ++++FFLDLLNTIATVSISAA+YGGN+QMGFKEMLV++RKMVAL+L+G++ TSLC +L
Sbjct: 121 LLTSIIFFLDLLNTIATVSISAAIYGGNSQMGFKEMLVQIRKMVALKLKGAIETSLCFIL 180

Query: 181 LASLTLLGFVALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAV 240
           LASLTLLG VAL  DF +++K   FM  DI ++SK +IFT  FGSLFVVLL KYIEWSAV
Sbjct: 181 LASLTLLGLVALSKDFFYITKDHPFMMSDIIFVSKFTIFTLFFGSLFVVLLGKYIEWSAV 240

Query: 241 WNMGIIVSILDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGS 300
           WNMGI++SILDKN+GYIAIGVASYLSRGSR+LGFSLMMVF VLKV FGLPCLYALWNEG+
Sbjct: 241 WNMGIVISILDKNKGYIAIGVASYLSRGSRRLGFSLMMVFLVLKVAFGLPCLYALWNEGN 300

Query: 301 CGVLGNVLFISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLEN-NGKAIKAVQQ 346
           CGVLGNV+F+ LNCVGNVVMWVVLMVYFYDCKREFLEKKVDLEN   KA +AVQQ
Sbjct: 301 CGVLGNVVFVILNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENTEKKAFEAVQQ 354

BLAST of Spg001869 vs. ExPASy TrEMBL
Match: A0A6J1FAX6 (uncharacterized protein LOC111442386 OS=Cucurbita moschata OX=3662 GN=LOC111442386 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 3.1e-129
Identity = 259/345 (75.07%), Postives = 289/345 (83.77%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           ME+HQILSKPKLGFSASFREA+KIL NHP FISLIIF S PLLASL AH+ LLHPTFIQL
Sbjct: 1   MENHQILSKPKLGFSASFREAFKILFNHPNFISLIIFLSLPLLASLSAHNILLHPTFIQL 60

Query: 61  LKLLDHEHPFNLYNIVAISCINTNGTARNCRPSVNGSFKATLSRKFLISTLLFSALVFFL 120
           L+ L H++P++L     I C   +GTA            +TLS  FLIS L+ SAL+FFL
Sbjct: 61  LRFLSHKNPYDLSLTSIIVCPILDGTA------CFSQLSSTLSHGFLISILVSSALIFFL 120

Query: 121 DLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLASLTLLGF 180
           DLLNTIA VSISAALYGGN+QMGFKEMLV+VRKMVAL+LRGSLATSLCSVLLASLTLLG 
Sbjct: 121 DLLNTIAIVSISAALYGGNSQMGFKEMLVQVRKMVALKLRGSLATSLCSVLLASLTLLGL 180

Query: 181 VALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNMGIIVSI 240
           VAL  DF  M+ G S MSI   ++ ++ IFT IFG+ FVVLL KY+EWSAVWNMGI++SI
Sbjct: 181 VALSKDFFLMATGSSHMSITNTFLLQLFIFTFIFGTPFVVLLGKYVEWSAVWNMGIVISI 240

Query: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGVLGNVLF 300
           LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKV+FG PCLYALWNE SCGV  NV+F
Sbjct: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVVFGFPCLYALWNERSCGVWENVMF 300

Query: 301 ISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENNGKAIKAVQQ 346
           +SLN VGNVVMWVVLMVYFYDCKRE LEKK+DLENNGK+I+AVQQ
Sbjct: 301 VSLNGVGNVVMWVVLMVYFYDCKRELLEKKIDLENNGKSIEAVQQ 339

BLAST of Spg001869 vs. ExPASy TrEMBL
Match: A0A6J1J592 (uncharacterized protein LOC111481871 OS=Cucurbita maxima OX=3661 GN=LOC111481871 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 1.0e-124
Identity = 252/345 (73.04%), Postives = 282/345 (81.74%), Query Frame = 0

Query: 1   MESHQILSKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPTFIQL 60
           ME+HQILSK KL FSASFREA+KIL NHP FISLIIF S PLLASL AH+ LLHPTFIQ 
Sbjct: 1   MENHQILSKSKLDFSASFREAFKILFNHPNFISLIIFLSLPLLASLSAHNILLHPTFIQF 60

Query: 61  LKLLDHEHPFNLYNIVAISCINTNGTARNCRPSVNGSFKATLSRKFLISTLLFSALVFFL 120
           L+ L H++P++      I C    GTA            +TLS  FLIS  + SAL+FFL
Sbjct: 61  LRFLSHKNPYDPSLTSIIMCQILEGTA------CFSQLSSTLSHGFLISIFVSSALIFFL 120

Query: 121 DLLNTIATVSISAALYGGNTQMGFKEMLVEVRKMVALRLRGSLATSLCSVLLASLTLLGF 180
           DLLNTIA VSISA+LYGGN+QMGFKEMLV+VRKMVAL+LRGSLATSLCSVLLASLTLLG 
Sbjct: 121 DLLNTIAIVSISASLYGGNSQMGFKEMLVQVRKMVALKLRGSLATSLCSVLLASLTLLGL 180

Query: 181 VALPTDFLFMSKGISFMSIDIFYISKVSIFTSIFGSLFVVLLAKYIEWSAVWNMGIIVSI 240
           VAL  DF  M+ G S MSI   ++ ++ IFT IFG+ FVVLL KY+EWSAVWNMGI++SI
Sbjct: 181 VALSKDFFLMATGSSHMSITNTFLLQLFIFTFIFGTPFVVLLGKYVEWSAVWNMGIVISI 240

Query: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCGVLGNVLF 300
           LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLK++FG PCLYALWNE SCGV  NV+F
Sbjct: 241 LDKNQGYIAIGVASYLSRGSRKLGFSLMMVFFVLKMVFGFPCLYALWNERSCGVWENVVF 300

Query: 301 ISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLENNGKAIKAVQQ 346
           +SLN VGNVVMWVVLMVYFYDCKRE LEKK+DLENNGK I+AVQQ
Sbjct: 301 VSLNGVGNVVMWVVLMVYFYDCKRELLEKKIDLENNGKPIEAVQQ 339

BLAST of Spg001869 vs. TAIR 10
Match: AT1G23840.1 (unknown protein; LOCATED IN: endomembrane system; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G23850.1); Has 53 Blast hits to 48 proteins in 5 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 53; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-21
Identity = 84/267 (31.46%), Postives = 138/267 (51.69%), Query Frame = 0

Query: 102 LSRKFLISTLLFSALVFF-----LDLLNTIATVSISAALYGGNTQ-MGFKEMLVEVRKMV 161
           LS   L+  L+ + L++F     LDLL T   V+ S+  Y    + +G   ++    K+ 
Sbjct: 71  LSENDLLPWLIQTTLLYFFPYTILDLLTTTTIVAASSIAYTSEEEPLGLLYLVGRSFKLC 130

Query: 162 ALRLRGSLATSLCSVLLASLTLLGFVALPTDFLFMSKGISFMSIDIFYISKV-------- 221
             ++ G L TSL  +LL++   LG  +  T +L+ +     +   IF+   V        
Sbjct: 131 QNKVGGCLITSLYVLLLSTSVFLGLFSGSTIYLYFAS--LTLEQQIFFNQAVVQDQRFLE 190

Query: 222 -------SIFTSIFGSLFVVLLAKYIEWSAVWNMGIIVSILDKNQ------GYIAIGVAS 281
                   +   I G++F+VL AK+ +WSA WN+ ++VS+L++ +      G  A+ +++
Sbjct: 191 QAVVLLDVVVVLIHGTVFIVLAAKFSKWSAGWNISMVVSVLEEEEDSKGIYGSSALSLSA 250

Query: 282 YLSRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNE--GSCGVLGNVLFISLNCVGNVVMW 340
           +  RG  K  F +M+VF V  ++  +PCLY   +E     GVL   L++SL CVGNVV W
Sbjct: 251 WYLRGQEKRDFWMMLVFLVGALVTRMPCLYYKCSESLSGNGVLYTGLYVSLICVGNVVKW 310

BLAST of Spg001869 vs. TAIR 10
Match: AT1G23850.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G23840.1); Has 47 Blast hits to 40 proteins in 5 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 47; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 91.3 bits (225), Expect = 1.7e-18
Identity = 77/264 (29.17%), Postives = 130/264 (49.24%), Query Frame = 0

Query: 107 LISTLLFSALVF-FLDLLNTIATVSISAALYGGNTQ-MGFKEMLVEVRKMVALRLRGSLA 166
           LI T L     +  +DL  T   VS S  ++    + + F +++    ++   RL G L 
Sbjct: 85  LIQTFLLYLFPYGLIDLFTTTTIVSASWTVHTSEEEPLRFGQLVRRTVEICQNRLEGCLI 144

Query: 167 TSLCSVLLASLTLLGFVALPTDFLFM------SKGISFMSIDI------FYISKV----- 226
           TSL  +LL++    GF+ + T++  +       +   + SI+I      +Y S       
Sbjct: 145 TSLYVLLLSTPVFFGFLFVATNYFHIISLTGSGENSYYYSINIEEDAEGYYRSSSVNSPV 204

Query: 227 -----SIFTSIFGSLFVVLLAKYIEWSAVWNMGIIVSILDKNQ------GYIAIGVASYL 286
                ++     G++F+ LLA + +WSA WNMG++VS+L++ +      G  A+ ++S  
Sbjct: 205 KMLFDAVLAMFHGAIFLGLLAMFSKWSAGWNMGLVVSVLEEEENGQSIYGTDALTLSSNY 264

Query: 287 SRGSRKLGFSLMMVFFVLKVLFGLPCLYALWNEGSCG--VLGNVLFISLNCVGNVVMWVV 339
            +G  K G  +M+VF V  +   +PC      E S G  VL    ++ L CVGN++ WV 
Sbjct: 265 GKGHEKRGLQVMLVFLVFAIAMRMPCFCFKCTESSNGNRVLYTSFYVGLICVGNMIKWVA 324

BLAST of Spg001869 vs. TAIR 10
Match: AT1G23830.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 12 plant structures; EXPRESSED DURING: 9 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G23840.1); Has 57 Blast hits to 52 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 57; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 90.5 bits (223), Expect = 2.8e-18
Identity = 98/359 (27.30%), Postives = 165/359 (45.96%), Query Frame = 0

Query: 8   SKPKLGFSASFREAWKILSNHPKFISLIIFFSFPLLASLLAHHTLLHPT------FIQLL 67
           S+ KL      + A K+L  +   +  +   S PL   L+     L  T      F+  L
Sbjct: 5   SEEKLSVIELLKRALKLLFGNINLLLFLCLCSLPLFFFLIFFELSLQTTVYLTSQFLWKL 64

Query: 68  KLLDHEHPFNLYNIVAISCINTNGTARNCRPSVNGSFKATLSRKFLISTLLFSALVFF-- 127
            +L  + P N  +++ IS                        +  LIS L+ + L++F  
Sbjct: 65  LILGEDLPEN--DLILIS----------------------EKKNDLISWLIQTFLLYFFP 124

Query: 128 ---LDLLNTIATVSISAALYGGNTQ-MGFKEMLVEVRKMVALRLRGSLATSLCSVLLASL 187
              LDLL T   V+ S+ +Y    + +G   ++    K+   R+ G L TSL  VLL S 
Sbjct: 125 YTILDLLTTTTIVAASSIVYTSKEEPLGLLYLVERSIKICQNRVGGCLITSL-YVLLWST 184

Query: 188 TLLGFVALPTDFLFMSKGISFMSIDI-------FYISKVSIFTSIFG-------SLFVVL 247
           ++  F  L     F+S   +++SI         F+     +F  +         +LF+VL
Sbjct: 185 SVFLFFFLFFFLQFLSGSTNYVSIPYLSREYKGFHYQPTGLFNVVVPLTLLMQCTLFIVL 244

Query: 248 LAKYIEWSAVWNMGIIVSILDKNQ------GYIAIGVASYLSRGSRKLGFSLMMVFFVLK 307
            AKY +WS+ WNMG++VS+L++++      G  A+ ++ +  +G  K    LM++F V  
Sbjct: 245 TAKYSKWSSGWNMGLVVSVLEEDEDGQGIYGGDALSLSGWYRKGHEKRDLWLMLMFLVFG 304

Query: 308 VLFGLPCLYALWNEGSCGVLGNVLFISLNCVGNVVMWVVLMVYFYDCKREFLEKKVDLE 335
           +   +PCLY+  +    GV+    ++ L CVGN++ WV  +  ++DCK   L KK D+E
Sbjct: 305 LATRMPCLYSKCSASGNGVMYTGFYVGLICVGNLLKWVTCLACYHDCKTMVLRKKRDVE 338