Spg001757 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg001757 vs. NCBI nr
Match: KAG6581060.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741.1 bits (1912), Expect = 6.1e-210
Identity = 353/380 (92.89%), Postives = 365/380 (96.05%), Query Frame = 0

Query: 112 QFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 171
           +FP M + AFLVLQV      ALA+PDCDFPV+FNFGDSNSDTGAISAAFEPIRWPYGDV
Sbjct: 2   EFPAMVVFAFLVLQVTF----ALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDV 61

Query: 172 FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 231
           FFNKPSGRDSDGRLI+DFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY
Sbjct: 62  FFNKPSGRDSDGRLIVDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 121

Query: 232 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLS 291
           EYGISPFFLDMQVTQFEQFKARSNDLYNQAK+PSDR+KLTRPEDFSKALYTFDIGQNDL+
Sbjct: 122 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLA 181

Query: 292 VGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP 351
           +GFRKLSIDQL AALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFY LNPAP
Sbjct: 182 MGFRKLSIDQLRAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAP 241

Query: 352 GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGF 411
           GILDEHGCIKAQNDISME NNQLKAKL+KLRAELPEAAITYVD YAAKYALISNGKTQGF
Sbjct: 242 GILDEHGCIKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGF 301

Query: 412 PDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANH 471
           P+PLKVCCGYHVRYDHVWCGTKA INGSLV GDACENRAQYVSWDGVHYSEAANHFVANH
Sbjct: 302 PNPLKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANH 361

Query: 472 ILNGSLSSPPIPITQACHRS 492
           ILNGSLSSPPIPIT+ACHRS
Sbjct: 362 ILNGSLSSPPIPITKACHRS 377

BLAST of Spg001757 vs. NCBI nr
Match: XP_038906270.1 (GDSL esterase/lipase At5g14450 [Benincasa hispida])

HSP 1 Score: 740.3 bits (1910), Expect = 1.0e-209
Identity = 353/397 (88.92%), Postives = 373/397 (93.95%), Query Frame = 0

Query: 94  NLKHSLSGTTLLPQFWQWQFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSD 153
           NL   +S TT      + +FP + +S FLVL V +GFS ALA+PDCDFPVIFNFGDSNSD
Sbjct: 16  NLLPEISCTT------KMEFPAIVVSPFLVLHVFLGFSLALASPDCDFPVIFNFGDSNSD 75

Query: 154 TGAISAAFEPIRWPYGDVFFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHG 213
           TGAISAAFEPIRWPYGDVFFNKPSGRDSDGRLIIDFIAE+LRLPYLSAYLNSLGANFRHG
Sbjct: 76  TGAISAAFEPIRWPYGDVFFNKPSGRDSDGRLIIDFIAEKLRLPYLSAYLNSLGANFRHG 135

Query: 214 ANFATGGSTVRKPNETIYEYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRP 273
           ANFATGGSTV+KPNETIYEYGISPFFLDMQVTQFEQFKARSNDLYNQAK+PSDRDKLTRP
Sbjct: 136 ANFATGGSTVQKPNETIYEYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDRDKLTRP 195

Query: 274 EDFSKALYTFDIGQNDLSVGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNT 333
           ED+SKALYTFDIGQNDL+VGFRKLSIDQL AALPDIVNQFASA+QR+YKLGGRSFWIHNT
Sbjct: 196 EDYSKALYTFDIGQNDLAVGFRKLSIDQLRAALPDIVNQFASALQRIYKLGGRSFWIHNT 255

Query: 334 GPFGCLPVNQFYILNPAPGILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYV 393
           GPFGCLPVNQFY LNP PGILD+HGCIKAQNDISME NNQLKAKLDKLR+ELPEAAITYV
Sbjct: 256 GPFGCLPVNQFYNLNPPPGILDQHGCIKAQNDISMELNNQLKAKLDKLRSELPEAAITYV 315

Query: 394 DAYAAKYALISNGKTQGFPDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYV 453
           D YAAKYALISNGKTQGFP+PLKVCCGYHVRYDHVWCGTKA INGSL+ GDACENRAQYV
Sbjct: 316 DVYAAKYALISNGKTQGFPEPLKVCCGYHVRYDHVWCGTKATINGSLMFGDACENRAQYV 375

Query: 454 SWDGVHYSEAANHFVANHILNGSLSSPPIPITQACHR 491
           SWDGVHYSEAANHFVANHILNGSLSSPPIPIT+AC+R
Sbjct: 376 SWDGVHYSEAANHFVANHILNGSLSSPPIPITKACYR 406

BLAST of Spg001757 vs. NCBI nr
Match: XP_022982673.1 (GDSL esterase/lipase At5g14450-like [Cucurbita maxima])

HSP 1 Score: 736.9 bits (1901), Expect = 1.2e-208
Identity = 350/380 (92.11%), Postives = 364/380 (95.79%), Query Frame = 0

Query: 112 QFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 171
           +FP M + AFLVLQV      ALA PDCDFPV+FNFGDSNSDTGAISAAFEPIRWPYGDV
Sbjct: 2   EFPAMVVFAFLVLQVTF----ALALPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDV 61

Query: 172 FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 231
           FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY
Sbjct: 62  FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 121

Query: 232 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLS 291
           EYGISPFFLDMQVTQFEQFKARSNDLYNQAK+PSDR+KLTRPEDFSKALYTFDIGQNDL+
Sbjct: 122 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLA 181

Query: 292 VGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP 351
           +GFRKLSIDQL AALP+IVNQFASAVQR+YKLGGRSFWIHNTGPFGCLPVNQFY LNPAP
Sbjct: 182 MGFRKLSIDQLRAALPEIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAP 241

Query: 352 GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGF 411
           GILDEHGCIKAQND+SME NNQLKAKL+KLRAELP+AAITYVD YAAKYALISNGKTQGF
Sbjct: 242 GILDEHGCIKAQNDVSMELNNQLKAKLEKLRAELPDAAITYVDVYAAKYALISNGKTQGF 301

Query: 412 PDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANH 471
           P+PLKVCCGYHVRYDHVWCGTKA INGSLV GDACENRAQYVSWDGVHYSEAANHFVANH
Sbjct: 302 PNPLKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANH 361

Query: 472 ILNGSLSSPPIPITQACHRS 492
           ILNGSLSSPPIPIT+ACHRS
Sbjct: 362 ILNGSLSSPPIPITKACHRS 377

BLAST of Spg001757 vs. NCBI nr
Match: XP_022935096.1 (GDSL esterase/lipase At5g14450-like [Cucurbita moschata])

HSP 1 Score: 734.9 bits (1896), Expect = 4.4e-208
Identity = 351/376 (93.35%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 116 MFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNK 175
           M + AFLVLQV      ALA+PDCDFPV+FNFGDSNSDTGAISAAFEPIRWPYGDVFFNK
Sbjct: 1   MVVFAFLVLQVTF----ALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFNK 60

Query: 176 PSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGI 235
           PSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGI
Sbjct: 61  PSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGI 120

Query: 236 SPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFR 295
           SPFFLDMQVTQFEQFKARSNDLYNQAK+PSDR+KLTRPEDFSKALYTFDIGQNDL++GFR
Sbjct: 121 SPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGFR 180

Query: 296 KLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGILD 355
           KLSIDQL AALPDIVNQFASAVQR+YKLGGRSFWIHNTGPFGCLPVNQFY LNPAPGILD
Sbjct: 181 KLSIDQLRAALPDIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAPGILD 240

Query: 356 EHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPL 415
           EHGCIKAQNDISME NNQLKAKL+KLRAELPEAAITYVD YAAKYALISNGKTQGFP+PL
Sbjct: 241 EHGCIKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGFPNPL 300

Query: 416 KVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANHILNG 475
           KVCCGYHVRYDHVWCGTKA INGSLV GDACENRAQYVSWDGVHYSEAANHFVANHILNG
Sbjct: 301 KVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILNG 360

Query: 476 SLSSPPIPITQACHRS 492
           SLSSPPIPIT+ACHRS
Sbjct: 361 SLSSPPIPITKACHRS 372

BLAST of Spg001757 vs. NCBI nr
Match: XP_023526085.1 (GDSL esterase/lipase At5g14450-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731.5 bits (1887), Expect = 4.8e-207
Identity = 349/380 (91.84%), Postives = 362/380 (95.26%), Query Frame = 0

Query: 112 QFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 171
           +FP   + A LVLQV      ALA+PDCDFPV+FNFGDSNSDTGAISAAFEPIRWPYGDV
Sbjct: 2   EFPAKVVFALLVLQVTF----ALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDV 61

Query: 172 FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 231
           FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTV+KPNETIY
Sbjct: 62  FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVQKPNETIY 121

Query: 232 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLS 291
           EYGISPFFLDMQVTQFEQFKARSNDLYNQAK+PSDR+KLTRPEDFSKALYTFDIGQNDL+
Sbjct: 122 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLA 181

Query: 292 VGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP 351
           +GFRKLSIDQL AALPDIVNQFASAVQR+YKL GRSFWIHNTGPFGCLPVNQFY LNPAP
Sbjct: 182 MGFRKLSIDQLRAALPDIVNQFASAVQRIYKLEGRSFWIHNTGPFGCLPVNQFYTLNPAP 241

Query: 352 GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGF 411
           GILDEHGCIKAQNDISME NNQLKAKL+KLRAELPEAAITYVD YAAKYALISNGKTQGF
Sbjct: 242 GILDEHGCIKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGF 301

Query: 412 PDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANH 471
           P+PLKVCCGYHVRYDHVWCGTKA INGSLV GDACENRAQYVSWDGVHYSEAANHFVANH
Sbjct: 302 PNPLKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANH 361

Query: 472 ILNGSLSSPPIPITQACHRS 492
           ILNGSLSSPPIPIT+ACHRS
Sbjct: 362 ILNGSLSSPPIPITKACHRS 377

BLAST of Spg001757 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 5.7e-150
Identity = 241/354 (68.08%), Postives = 288/354 (81.36%), Query Frame = 0

Query: 137 PDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGRDSDGRLIIDFIAERLRL 196
           P C FP I+NFGDSNSDTG ISAAFEPIR PYG  FF++P+GRDSDGRL IDFIAERL L
Sbjct: 35  PTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDGRLTIDFIAERLGL 94

Query: 197 PYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFFLDMQVTQFEQFKARSND 256
           PYLSAYLNSLG+NFRHGANFATGGST+R+ NETI++YGISPF LDMQ+ QF+QFKARS  
Sbjct: 95  PYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQIAQFDQFKARSAL 154

Query: 257 LYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFRKLSIDQLHAALPDIVNQFASA 316
           L+ Q K   DR+KL R E+F+KALYTFDIGQNDLSVGFR +S+DQL A +PDIVN  ASA
Sbjct: 155 LFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSVDQLKATIPDIVNHLASA 214

Query: 317 VQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGILDEHGCIKAQNDISMEFNNQLKA 376
           V+ +Y+ GGR+FW+HNTGPFGCLPVN FY+  PAPG LD+ GC+KAQN+++MEFN +LK 
Sbjct: 215 VRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVKAQNEMAMEFNRKLKE 274

Query: 377 KLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGYHVRYDHVWCGTKAMI 436
            +  LR EL +AAITYVD Y AKY ++SN K  GF +PLKVCCGYH +YDH+WCG K  +
Sbjct: 275 TVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGYHEKYDHIWCGNKGKV 334

Query: 437 NGSLVSGDACENRAQYVSWDGVHYSEAANHFVANHILNGSLSSPPIPITQACHR 491
           N + + G +C N    VSWDGVHY+EAAN  VA+  LNG L+ PP+PIT+AC+R
Sbjct: 335 NNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPPVPITRACYR 388

BLAST of Spg001757 vs. ExPASy Swiss-Prot
Match: Q9LII9 (GDSL esterase/lipase At3g27950 OS=Arabidopsis thaliana OX=3702 GN=At3g27950 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 4.1e-100
Identity = 186/369 (50.41%), Postives = 245/369 (66.40%), Query Frame = 0

Query: 123 VLQVAVGFSPALAA--PDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGRD 182
           ++ + +GF+  L+A    C+FP +FNFGDSNSDTGAISAA   +  P G  FF + +GR 
Sbjct: 10  IIVLLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGRH 69

Query: 183 SDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFFL 242
           SDGRLIIDFI E L LPYL+ YL+S+GAN+RHGANFATGGS +R P    +    SPF L
Sbjct: 70  SDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIR-PTLACF----SPFHL 129

Query: 243 DMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFRKLSID 302
             QV+QF  FK R+  LYNQ     +R  L+    FSKALYT DIGQNDL++GF+ ++ +
Sbjct: 130 GTQVSQFIHFKTRTLSLYNQTNGKFNR--LSHTNYFSKALYTLDIGQNDLAIGFQNMTEE 189

Query: 303 QLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGI-LDEHGC 362
           QL A +P I+  F  A++ LYK G R F IHNTGP GCLP    Y+L   P I  D +GC
Sbjct: 190 QLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLP----YLLKAFPAIPRDPYGC 249

Query: 363 IKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCC 422
           +K  N++++EFN QLK K+ +L+ ELP +  TYVD Y+AKY LI+  K  GF DP   CC
Sbjct: 250 LKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYCC 309

Query: 423 GYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANHILNGSLSS 482
              +    + CG    +NG+ +   +C+NR  ++SWDG+HY+E AN  VAN IL+GS+S 
Sbjct: 310 VGAIGRG-MGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISD 366

Query: 483 PPIPITQAC 489
           PP+P  +AC
Sbjct: 370 PPLPTQKAC 366

BLAST of Spg001757 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 5.9e-99
Identity = 181/376 (48.14%), Postives = 247/376 (65.69%), Query Frame = 0

Query: 123 VLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGRDSD 182
           VL  +    P   +P C+FP IFNFGDSNSDTG +SA+F    +P G  FF+ PSGR SD
Sbjct: 11  VLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGRFSD 70

Query: 183 GRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFFLDM 242
           GRLIIDFIAE L LPYL+A+L+S+G+NF HGANFAT GSTVR PN TI + G+SP  LD+
Sbjct: 71  GRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPISLDV 130

Query: 243 QVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFR-KLSIDQ 302
           Q+ QF  F  RS  + N  +    +  L + E FS+ALYTFDIGQNDL+ G +  ++ DQ
Sbjct: 131 QLVQFSDFITRSQLIRN--RGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNMTSDQ 190

Query: 303 LHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILN--PAPGI-LDEHG 362
           + A +PD+ +Q ++ ++++Y  GGR FWIHNT P GCLP    Y+L+  P P   +D HG
Sbjct: 191 IKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLP----YVLDRFPVPASQIDNHG 250

Query: 363 CIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVC 422
           C   +N+I+  +N++LK ++ +LR EL EAA TYVD Y+ K  LI+  K  GF  PL  C
Sbjct: 251 CAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVAC 310

Query: 423 CGYHVRYDH---VWCGTKAMING-SLVSGDACENRAQYVSWDGVHYSEAANHFVANHILN 482
           CG+  +Y+    + CG K MI G  +V   +C + +  VSWDG+H++E  N ++   I +
Sbjct: 311 CGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIND 370

Query: 483 GSLSSPPIPITQACHR 491
           G+ S PP+P+  AC R
Sbjct: 371 GAFSDPPLPVKSACTR 380

BLAST of Spg001757 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.6e-96
Identity = 190/377 (50.40%), Postives = 241/377 (63.93%), Query Frame = 0

Query: 122 LVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGRDS 181
           L L +A+  S A A  DC FP +FNFGDSNSDTG +S+ F     P G  FF  P+GR  
Sbjct: 21  LALALALRPSDAGAGGDCHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGMPAGRYC 80

Query: 182 DGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFFLD 241
           DGRL+IDFIAE L L +LSAYLNS+G+NF  GANFAT GS++R+ N +++  G SP  LD
Sbjct: 81  DGRLVIDFIAESLGLTHLSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGFSPISLD 140

Query: 242 MQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDL-SVGFRKLSID 301
           +Q  +FEQF  RS  +YN  K    R+ L R E FS+ALYTFDIGQND+ S  F   + +
Sbjct: 141 VQFWEFEQFINRSQLVYNN-KGGIYREILPRAEYFSQALYTFDIGQNDITSSYFVNNTTE 200

Query: 302 QLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGI---LDEH 361
           ++ A +PD++ +  S +Q +Y  GGR FWIHNTGP GCLP    Y L   P +    D  
Sbjct: 201 EVEAIIPDLMERLTSIIQSVYSRGGRYFWIHNTGPLGCLP----YALLHRPDLAIPADGT 260

Query: 362 GCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPLKV 421
           GC    N ++  FN +LK  +  LR   P+AA TYVD Y AKY LIS     GF DPL  
Sbjct: 261 GCSVTYNKVAQLFNLRLKETVASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLLT 320

Query: 422 CCGY-HVRYD---HVWCGTKAMING-SLVSGDACENRAQYVSWDGVHYSEAANHFVANHI 481
           CCGY   RY+    V CG K  +NG S+V G +CEN ++ VSWDGVH++EAAN FV + I
Sbjct: 321 CCGYGGGRYNLDLSVGCGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQI 380

Query: 482 LNGSLSSPPIPITQACH 490
           + G+LS PP+ + QACH
Sbjct: 381 VAGALSDPPVALRQACH 392

BLAST of Spg001757 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 4.0e-95
Identity = 181/386 (46.89%), Postives = 249/386 (64.51%), Query Frame = 0

Query: 114 PVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFF 173
           P++ LS  L +      S A A+  CDFP IFNFGDSNSDTG  +AAF P+  PYG+ FF
Sbjct: 10  PIITLSFLLCM-----LSLAYASETCDFPAIFNFGDSNSDTGGKAAAFYPLNPPYGETFF 69

Query: 174 NKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEY 233
           ++ +GR SDGRLIIDFIAE   LPYLS YL+SLG+NF+HGA+FAT GST++ P   I  +
Sbjct: 70  HRSTGRYSDGRLIIDFIAESFNLPYLSPYLSSLGSNFKHGADFATAGSTIKLPTTIIPAH 129

Query: 234 -GISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPED--FSKALYTFDIGQNDL 293
            G SPF+LD+Q +QF QF  RS  +    ++         PE+  F KALYTFDIGQNDL
Sbjct: 130 GGFSPFYLDVQYSQFRQFIPRSQFI----RETGGIFAELVPEEYYFEKALYTFDIGQNDL 189

Query: 294 SVGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPA 353
           + GF  L++++++A +PD+VN F++ V+++Y LG R+FWIHNTGP GCL     +IL   
Sbjct: 190 TEGFLNLTVEEVNATVPDLVNSFSANVKKIYDLGARTFWIHNTGPIGCLS----FILTYF 249

Query: 354 P-GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQ 413
           P    D  GC KA N+++  FN++LK  + +LR +LP A   +VD Y+ KY+L S  +  
Sbjct: 250 PWAEKDSAGCAKAYNEVAQHFNHKLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPEKH 309

Query: 414 GFPDPLKVCCGYHVRYDH---VWCG-TKAMINGSLVSGDACENRAQYVSWDGVHYSEAAN 473
           GF  PL  CCGY  +Y+      CG T    +G+ +   +C   +  V+WDG HY+EAAN
Sbjct: 310 GFEFPLITCCGYGGKYNFSVTAPCGDTVTADDGTKIVVGSCACPSVRVNWDGAHYTEAAN 369

Query: 474 HFVANHILNGSLSSPPIPITQACHRS 492
            +  + I  G+ S PP+P+  ACH++
Sbjct: 370 EYFFDQISTGAFSDPPVPLNMACHKT 382

BLAST of Spg001757 vs. ExPASy TrEMBL
Match: A0A6J1J5G0 (GDSL esterase/lipase At5g14450-like OS=Cucurbita maxima OX=3661 GN=LOC111481477 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.6e-209
Identity = 350/380 (92.11%), Postives = 364/380 (95.79%), Query Frame = 0

Query: 112 QFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 171
           +FP M + AFLVLQV      ALA PDCDFPV+FNFGDSNSDTGAISAAFEPIRWPYGDV
Sbjct: 2   EFPAMVVFAFLVLQVTF----ALALPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDV 61

Query: 172 FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 231
           FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY
Sbjct: 62  FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 121

Query: 232 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLS 291
           EYGISPFFLDMQVTQFEQFKARSNDLYNQAK+PSDR+KLTRPEDFSKALYTFDIGQNDL+
Sbjct: 122 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLA 181

Query: 292 VGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP 351
           +GFRKLSIDQL AALP+IVNQFASAVQR+YKLGGRSFWIHNTGPFGCLPVNQFY LNPAP
Sbjct: 182 MGFRKLSIDQLRAALPEIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAP 241

Query: 352 GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGF 411
           GILDEHGCIKAQND+SME NNQLKAKL+KLRAELP+AAITYVD YAAKYALISNGKTQGF
Sbjct: 242 GILDEHGCIKAQNDVSMELNNQLKAKLEKLRAELPDAAITYVDVYAAKYALISNGKTQGF 301

Query: 412 PDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANH 471
           P+PLKVCCGYHVRYDHVWCGTKA INGSLV GDACENRAQYVSWDGVHYSEAANHFVANH
Sbjct: 302 PNPLKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANH 361

Query: 472 ILNGSLSSPPIPITQACHRS 492
           ILNGSLSSPPIPIT+ACHRS
Sbjct: 362 ILNGSLSSPPIPITKACHRS 377

BLAST of Spg001757 vs. ExPASy TrEMBL
Match: A0A6J1F3K8 (GDSL esterase/lipase At5g14450-like OS=Cucurbita moschata OX=3662 GN=LOC111442066 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 2.1e-208
Identity = 351/376 (93.35%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 116 MFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNK 175
           M + AFLVLQV      ALA+PDCDFPV+FNFGDSNSDTGAISAAFEPIRWPYGDVFFNK
Sbjct: 1   MVVFAFLVLQVTF----ALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFNK 60

Query: 176 PSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGI 235
           PSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGI
Sbjct: 61  PSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGI 120

Query: 236 SPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFR 295
           SPFFLDMQVTQFEQFKARSNDLYNQAK+PSDR+KLTRPEDFSKALYTFDIGQNDL++GFR
Sbjct: 121 SPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGFR 180

Query: 296 KLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGILD 355
           KLSIDQL AALPDIVNQFASAVQR+YKLGGRSFWIHNTGPFGCLPVNQFY LNPAPGILD
Sbjct: 181 KLSIDQLRAALPDIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAPGILD 240

Query: 356 EHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPL 415
           EHGCIKAQNDISME NNQLKAKL+KLRAELPEAAITYVD YAAKYALISNGKTQGFP+PL
Sbjct: 241 EHGCIKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGFPNPL 300

Query: 416 KVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANHILNG 475
           KVCCGYHVRYDHVWCGTKA INGSLV GDACENRAQYVSWDGVHYSEAANHFVANHILNG
Sbjct: 301 KVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILNG 360

Query: 476 SLSSPPIPITQACHRS 492
           SLSSPPIPIT+ACHRS
Sbjct: 361 SLSSPPIPITKACHRS 372

BLAST of Spg001757 vs. ExPASy TrEMBL
Match: A0A5D3D5A1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00280 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 9.9e-206
Identity = 346/380 (91.05%), Postives = 360/380 (94.74%), Query Frame = 0

Query: 112 QFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 171
           +F    LSAFLV  V V    ALA+PDC+FPVIFNFGDSNSDTGAISAAFEPIRWPYGDV
Sbjct: 2   EFSATALSAFLVFHVFV----ALASPDCNFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 61

Query: 172 FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 231
           FFNKPSGRDSDGRLIIDFIAE+LRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY
Sbjct: 62  FFNKPSGRDSDGRLIIDFIAEKLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 121

Query: 232 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLS 291
           EYGISPFFLDMQVTQFEQFKARSNDLYNQAK+P DR+KLTRPED+SKALYTFDIGQNDL+
Sbjct: 122 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPYDREKLTRPEDYSKALYTFDIGQNDLA 181

Query: 292 VGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP 351
           VGFRKLSIDQL AALPDIVNQFASA+QR+YKLGGRSFWIHNTGPFGCLPVNQFY LNP P
Sbjct: 182 VGFRKLSIDQLRAALPDIVNQFASAIQRIYKLGGRSFWIHNTGPFGCLPVNQFYNLNPPP 241

Query: 352 GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGF 411
           GILDEHGCIKAQNDISME NNQLKAKLDKLRAELP+AAITYVD YAAKYALISNGKTQGF
Sbjct: 242 GILDEHGCIKAQNDISMELNNQLKAKLDKLRAELPDAAITYVDVYAAKYALISNGKTQGF 301

Query: 412 PDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANH 471
           P+PLKVCCGYHVRYDHVWCGTKA ING LV GDACENRAQYVSWDGVHYSEAANHFVANH
Sbjct: 302 PEPLKVCCGYHVRYDHVWCGTKAPINGRLVFGDACENRAQYVSWDGVHYSEAANHFVANH 361

Query: 472 ILNGSLSSPPIPITQACHRS 492
           ILNG+LSSPPIPITQACHR+
Sbjct: 362 ILNGALSSPPIPITQACHRN 377

BLAST of Spg001757 vs. ExPASy TrEMBL
Match: A0A1S3CMP9 (GDSL esterase/lipase At5g14450-like OS=Cucumis melo OX=3656 GN=LOC103502685 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 9.9e-206
Identity = 346/380 (91.05%), Postives = 360/380 (94.74%), Query Frame = 0

Query: 112 QFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 171
           +F    LSAFLV  V V    ALA+PDC+FPVIFNFGDSNSDTGAISAAFEPIRWPYGDV
Sbjct: 2   EFSATALSAFLVFHVFV----ALASPDCNFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 61

Query: 172 FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 231
           FFNKPSGRDSDGRLIIDFIAE+LRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY
Sbjct: 62  FFNKPSGRDSDGRLIIDFIAEKLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 121

Query: 232 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLS 291
           EYGISPFFLDMQVTQFEQFKARSNDLYNQAK+P DR+KLTRPED+SKALYTFDIGQNDL+
Sbjct: 122 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPYDREKLTRPEDYSKALYTFDIGQNDLA 181

Query: 292 VGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP 351
           VGFRKLSIDQL AALPDIVNQFASA+QR+YKLGGRSFWIHNTGPFGCLPVNQFY LNP P
Sbjct: 182 VGFRKLSIDQLRAALPDIVNQFASAIQRIYKLGGRSFWIHNTGPFGCLPVNQFYNLNPPP 241

Query: 352 GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGF 411
           GILDEHGCIKAQNDISME NNQLKAKLDKLRAELP+AAITYVD YAAKYALISNGKTQGF
Sbjct: 242 GILDEHGCIKAQNDISMELNNQLKAKLDKLRAELPDAAITYVDVYAAKYALISNGKTQGF 301

Query: 412 PDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANH 471
           P+PLKVCCGYHVRYDHVWCGTKA ING LV GDACENRAQYVSWDGVHYSEAANHFVANH
Sbjct: 302 PEPLKVCCGYHVRYDHVWCGTKAPINGRLVFGDACENRAQYVSWDGVHYSEAANHFVANH 361

Query: 472 ILNGSLSSPPIPITQACHRS 492
           ILNG+LSSPPIPITQACHR+
Sbjct: 362 ILNGALSSPPIPITQACHRN 377

BLAST of Spg001757 vs. ExPASy TrEMBL
Match: A0A0A0LI33 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G840420 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.2e-205
Identity = 346/380 (91.05%), Postives = 360/380 (94.74%), Query Frame = 0

Query: 112 QFPVMFLSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 171
           +F    LSAFLV  V V    ALAAPDC+FPVIFNFGDSNSDTGAISAAFEPIRWPYGDV
Sbjct: 2   EFTATALSAFLVFHVFV----ALAAPDCNFPVIFNFGDSNSDTGAISAAFEPIRWPYGDV 61

Query: 172 FFNKPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 231
           FFNKPSGRDSDGRLIIDFIAE+LRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY
Sbjct: 62  FFNKPSGRDSDGRLIIDFIAEKLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIY 121

Query: 232 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLS 291
           EYGISPFFLDMQVTQFEQFKARSNDLYNQAK+P DR+KLTRPED+SKALYTFDIGQNDL+
Sbjct: 122 EYGISPFFLDMQVTQFEQFKARSNDLYNQAKNPYDREKLTRPEDYSKALYTFDIGQNDLA 181

Query: 292 VGFRKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP 351
           VGFRKLSIDQL AALPDI NQFASA+QR+YKLGGRSFWIHNTGPFGCLPVNQFY LNP P
Sbjct: 182 VGFRKLSIDQLRAALPDIANQFASAIQRIYKLGGRSFWIHNTGPFGCLPVNQFYNLNPPP 241

Query: 352 GILDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGF 411
           GILDEHGCIKAQNDISME NNQLKAKLDKLRAELP+AAITYVD YAAKYALISNGKTQGF
Sbjct: 242 GILDEHGCIKAQNDISMELNNQLKAKLDKLRAELPDAAITYVDVYAAKYALISNGKTQGF 301

Query: 412 PDPLKVCCGYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANH 471
           P+PLKVCCGYHVRYDHVWCGTKA INGSLV GDACENRAQYVSWDGVHYSEAANHFVA+H
Sbjct: 302 PEPLKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVASH 361

Query: 472 ILNGSLSSPPIPITQACHRS 492
           IL+GSLSSPPIPITQACHR+
Sbjct: 362 ILSGSLSSPPIPITQACHRN 377

BLAST of Spg001757 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 532.3 bits (1370), Expect = 4.0e-151
Identity = 241/354 (68.08%), Postives = 288/354 (81.36%), Query Frame = 0

Query: 137 PDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGRDSDGRLIIDFIAERLRL 196
           P C FP I+NFGDSNSDTG ISAAFEPIR PYG  FF++P+GRDSDGRL IDFIAERL L
Sbjct: 35  PTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDGRLTIDFIAERLGL 94

Query: 197 PYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFFLDMQVTQFEQFKARSND 256
           PYLSAYLNSLG+NFRHGANFATGGST+R+ NETI++YGISPF LDMQ+ QF+QFKARS  
Sbjct: 95  PYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQIAQFDQFKARSAL 154

Query: 257 LYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFRKLSIDQLHAALPDIVNQFASA 316
           L+ Q K   DR+KL R E+F+KALYTFDIGQNDLSVGFR +S+DQL A +PDIVN  ASA
Sbjct: 155 LFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSVDQLKATIPDIVNHLASA 214

Query: 317 VQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGILDEHGCIKAQNDISMEFNNQLKA 376
           V+ +Y+ GGR+FW+HNTGPFGCLPVN FY+  PAPG LD+ GC+KAQN+++MEFN +LK 
Sbjct: 215 VRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVKAQNEMAMEFNRKLKE 274

Query: 377 KLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGYHVRYDHVWCGTKAMI 436
            +  LR EL +AAITYVD Y AKY ++SN K  GF +PLKVCCGYH +YDH+WCG K  +
Sbjct: 275 TVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGYHEKYDHIWCGNKGKV 334

Query: 437 NGSLVSGDACENRAQYVSWDGVHYSEAANHFVANHILNGSLSSPPIPITQACHR 491
           N + + G +C N    VSWDGVHY+EAAN  VA+  LNG L+ PP+PIT+AC+R
Sbjct: 335 NNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPPVPITRACYR 388

BLAST of Spg001757 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-100
Identity = 185/369 (50.14%), Postives = 242/369 (65.58%), Query Frame = 0

Query: 123 VLQVAVGFSPALAA--PDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGRD 182
           ++ + +GF+  L+A    C+FP +FNFGDSNSDTGAISAA   +  P G  FF + +GR 
Sbjct: 10  IIVLLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGRH 69

Query: 183 SDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFFL 242
           SDGRLIIDFI E L LPYL+ YL+S+GAN+RHGANFATGGS +R P    +    SPF L
Sbjct: 70  SDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIR-PTLACF----SPFHL 129

Query: 243 DMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFRKLSID 302
             QV+QF  FK R+  LYNQ              DFSKALYT DIGQNDL++GF+ ++ +
Sbjct: 130 GTQVSQFIHFKTRTLSLYNQT------------NDFSKALYTLDIGQNDLAIGFQNMTEE 189

Query: 303 QLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGI-LDEHGC 362
           QL A +P I+  F  A++ LYK G R F IHNTGP GCLP    Y+L   P I  D +GC
Sbjct: 190 QLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLP----YLLKAFPAIPRDPYGC 249

Query: 363 IKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCC 422
           +K  N++++EFN QLK K+ +L+ ELP +  TYVD Y+AKY LI+  K  GF DP   CC
Sbjct: 250 LKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYCC 309

Query: 423 GYHVRYDHVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVANHILNGSLSS 482
              +    + CG    +NG+ +   +C+NR  ++SWDG+HY+E AN  VAN IL+GS+S 
Sbjct: 310 VGAIGRG-MGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISD 356

Query: 483 PPIPITQAC 489
           PP+P  +AC
Sbjct: 370 PPLPTQKAC 356

BLAST of Spg001757 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 362.8 bits (930), Expect = 4.2e-100
Identity = 181/376 (48.14%), Postives = 247/376 (65.69%), Query Frame = 0

Query: 123 VLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGRDSD 182
           VL  +    P   +P C+FP IFNFGDSNSDTG +SA+F    +P G  FF+ PSGR SD
Sbjct: 11  VLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGRFSD 70

Query: 183 GRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFFLDM 242
           GRLIIDFIAE L LPYL+A+L+S+G+NF HGANFAT GSTVR PN TI + G+SP  LD+
Sbjct: 71  GRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPISLDV 130

Query: 243 QVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVGFR-KLSIDQ 302
           Q+ QF  F  RS  + N  +    +  L + E FS+ALYTFDIGQNDL+ G +  ++ DQ
Sbjct: 131 QLVQFSDFITRSQLIRN--RGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNMTSDQ 190

Query: 303 LHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILN--PAPGI-LDEHG 362
           + A +PD+ +Q ++ ++++Y  GGR FWIHNT P GCLP    Y+L+  P P   +D HG
Sbjct: 191 IKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLP----YVLDRFPVPASQIDNHG 250

Query: 363 CIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVC 422
           C   +N+I+  +N++LK ++ +LR EL EAA TYVD Y+ K  LI+  K  GF  PL  C
Sbjct: 251 CAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVAC 310

Query: 423 CGYHVRYDH---VWCGTKAMING-SLVSGDACENRAQYVSWDGVHYSEAANHFVANHILN 482
           CG+  +Y+    + CG K MI G  +V   +C + +  VSWDG+H++E  N ++   I +
Sbjct: 311 CGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIND 370

Query: 483 GSLSSPPIPITQACHR 491
           G+ S PP+P+  AC R
Sbjct: 371 GAFSDPPLPVKSACTR 380

BLAST of Spg001757 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 332.8 bits (852), Expect = 4.7e-91
Identity = 171/381 (44.88%), Postives = 236/381 (61.94%), Query Frame = 0

Query: 118 LSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPS 177
           LS+   L +    SP+  A  C FP IFNFGDSNSDTG +SAAF     P+G  FF  P+
Sbjct: 5   LSSLFALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPA 64

Query: 178 GRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISP 237
           GR  DGRL+IDFIAE L LPYLSA+L+S+G+NF HGANFAT GS +R  N T+ + G SP
Sbjct: 65  GRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSP 124

Query: 238 FFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPEDFSKALYTFDIGQNDLSVG-FRK 297
           F LD+Q  QF  F  RS  +  +++    +  L   + FSKALYTFDIGQNDL+ G F  
Sbjct: 125 FSLDVQFVQFYNFHNRSQTV--RSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFAN 184

Query: 298 LSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAP---GI 357
            +++Q+   +P+I++QF +A++ +Y  GGR FWIHNTGP GCL     Y++   P     
Sbjct: 185 KTVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLA----YVIERFPNKASD 244

Query: 358 LDEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPD 417
            D HGC+   N ++ +FN+ LK  + +LR+ L EAAITYVD Y+ K+ L  + +  GF  
Sbjct: 245 FDSHGCVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKG 304

Query: 418 PLKVCCGYHVRYDH---VWCGTKAMINGSLV-SGDACENRAQYVSWDGVHYSEAANHFVA 477
            L  CCG+  +Y++   + CG K ++ G  V  G  C+   + V WDGVH+++AAN F+ 
Sbjct: 305 SLVSCCGHGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIF 364

Query: 478 NHILNGSLSSPPIPITQACHR 491
           + I  G        +++AC R
Sbjct: 365 DKIAPG--------LSKACKR 371

BLAST of Spg001757 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 8.0e-83
Identity = 161/371 (43.40%), Postives = 229/371 (61.73%), Query Frame = 0

Query: 118 LSAFLVLQVAVGFSPALAAPDCDFPVIFNFGDSNSDTG-AISAAFEPIRWPYGDVFFNKP 177
           LS  L + +++ F P+  +    +P I NFGDSNSDTG  ISA  E +  PYG  +FN P
Sbjct: 8   LSIVLFILISL-FLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLP 67

Query: 178 SGRDSDGRLIIDFIAERLRLPYLSAYLNSLG-ANFRHGANFATGGSTVRKPNETIYEYGI 237
           SGR  DGRLI+DF+ + + LP+L+ YL+SLG  NF+ G NFA  GST+   N T     +
Sbjct: 68  SGRYCDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPT----SV 127

Query: 238 SPFFLDMQVTQFEQFKARSNDLYNQAKDPSDRDKLTRPED-FSKALYTFDIGQNDLSVGF 297
           SPF  D+Q++QF +FK+R+ +L   +K     +K   P D +SK LY  DIGQND++  F
Sbjct: 128 SPFSFDLQISQFIRFKSRAIELL--SKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAF 187

Query: 298 RKLSIDQLHAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYILNPAPGIL 357
              ++DQ+ A++P I+  F + ++RLY+ GGR+ WIHNTGP GCL  N           L
Sbjct: 188 YSKTLDQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQN-IAKFGTDSTKL 247

Query: 358 DEHGCIKAQNDISMEFNNQLKAKLDKLRAELPEAAITYVDAYAAKYALISNGKTQGFPDP 417
           DE GC+ + N  +  FN QL A  +K +A+ P+A +TYVD ++ K  LI+N    GF  P
Sbjct: 248 DEFGCVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKP 307

Query: 418 LKVCCGY---HVRYD-HVWCGTKAMINGSLVSGDACENRAQYVSWDGVHYSEAANHFVAN 477
           L  CCG     + YD  + CG   +++G  V+  AC + ++Y++WDG+HY+EAAN FV++
Sbjct: 308 LMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSS 367

Query: 478 HILNGSLSSPP 482
            IL G  S PP
Sbjct: 368 QILTGKYSDPP 370